AT3G21490

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0011957
Physical Location & Seq
Forward (+)
7573065 .. 7573669
605 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G21490.1

Sequence Viewer

Length: 411 bp
ATGACGAAAGATAAGAAGAAAAAGGACAATGTCAGATATATGGATGTCGAATTCAATGTATCGATGCATTGCAACGACTGCGAGAGAAAAATCGCTAGAGTTATTTCCAAATTCAAAGGAGTTGAAACGTTTGTGACGGATATGATTAACCACAAGGTTGTGGTCAGGGGAAAGATTGATCCGAACAAGTTGTTGAAGAAGCTTAAGAAGAAGACTGGCAAAAGAGTGAAGATTGTAGTTAAGGAAGAGAAAGGTGAAGAATCTAGTAAGGAGAACGAAAATGTTCTCGAGATTGATATGGAATCGATTTGTTTAGGGGATCAGTCGATGTTCGGGTTTTGTGATTGGGAGATGGAGAAATTTATGGTGTTTAGTGATGAAAACGTCAAAGCCATTTGTTCTATTTCTTAG

Protein Analysis

136

Amino Acids

15.81

Weight (kDa)

8.74

Isoelectric Point (pI)

27.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 18 - 74 4e-14 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011957)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G21490
fragaria_vesca FvH4_3g29750 FvH4_4g16960 FvH4_4g16960 FvH4_4g16960 FvH4_4g16960
malus_domestica MD16G1177100.v1.1
prunus_persica Prupe.1G144000_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0420551
rosa_laevigata RLG00000007752
rosa_multiflora Rmu_sc0002248.1_g000057
rosa_roxburghii Rroxscaffold_5G00363170
rosa_rugosa Rorug04G0164800 Rorug04G0164900 Rorug04G0165000
rosa_samantha Rh4AG226400 Rh4CG241200 Rh4DG224700
rosa_wichuraiana Rw4G019550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 128
AclWI GGATC 2 cut(s) 173, 327
AcsI RAATTY 3 cut(s) 50, 110, 359
AflII CTTAAG 1 cut(s) 203
AgsI TTSAA 4 cut(s) 55, 115, 125, 196
AluBI AGCT 1 cut(s) 202
AluI AGCT 1 cut(s) 202
AlwI GGATC 2 cut(s) 173, 327
Ama87I CYCGRG 1 cut(s) 287
ApoI RAATTY 3 cut(s) 50, 110, 359
Asp700I GAANNNNTTC 1 cut(s) 282
AsuHPI GGTGA 1 cut(s) 266
AvaI CYCGRG 1 cut(s) 287
BbsI GAAGAC 1 cut(s) 218
BccI CCATC 1 cut(s) 346
BfaI CTAG 2 cut(s) 96, 264
BfrI CTTAAG 1 cut(s) 203
BmeT110I CYCGRG 1 cut(s) 287
BmsI GCATC 1 cut(s) 54
BpiI GAAGAC 1 cut(s) 218
Bsa29I ATCGAT 2 cut(s) 62, 305
Bse1I ACTGG 1 cut(s) 220
Bse3DI GCAATG 1 cut(s) 67
BseCI ATCGAT 2 cut(s) 62, 305
BseGI GGATG 1 cut(s) 49
BseMI GCAATG 1 cut(s) 67
BseNI ACTGG 1 cut(s) 220
BshVI ATCGAT 2 cut(s) 62, 305
BsiHKCI CYCGRG 1 cut(s) 287
BsoBI CYCGRG 1 cut(s) 287
Bsp143I GATC 2 cut(s) 178, 319
BspDI ATCGAT 2 cut(s) 62, 305
BspPI GGATC 2 cut(s) 173, 327
BspTI CTTAAG 1 cut(s) 203
BsrDI GCAATG 1 cut(s) 67
BsrI ACTGG 1 cut(s) 220
BssMI GATC 2 cut(s) 178, 319
Bst6I CTCTTC 1 cut(s) 240
BstAFI CTTAAG 1 cut(s) 203
BstAPI GCANNNNNTGC 1 cut(s) 78
BstDEI CTNAG 1 cut(s) 408
BstF5I GGATG 1 cut(s) 49
BstKTI GATC 2 cut(s) 181, 322
BstMBI GATC 2 cut(s) 178, 319
BstMWI GCNNNNNNNGC 1 cut(s) 78
BstV2I GAAGAC 1 cut(s) 218
Bsu15I ATCGAT 2 cut(s) 62, 305
BsuTUI ATCGAT 2 cut(s) 62, 305
BtsCI GGATG 1 cut(s) 49
ClaI ATCGAT 2 cut(s) 62, 305
CviJI RGCY 2 cut(s) 202, 392
CviKI_1 RGCY 2 cut(s) 202, 392
DdeI CTNAG 1 cut(s) 408
DpnI GATC 2 cut(s) 180, 321
DpnII GATC 2 cut(s) 178, 319
Eam1104I CTCTTC 1 cut(s) 240
EarI CTCTTC 1 cut(s) 240
Eco88I CYCGRG 1 cut(s) 287
EcoRI GAATTC 1 cut(s) 50
EcoT22I ATGCAT 1 cut(s) 69
FaiI YATR 5 cut(s) 39, 41, 143, 299, 365
FokI GGATG 1 cut(s) 56
FspBI CTAG 2 cut(s) 96, 264
HindIII AAGCTT 1 cut(s) 200
HinfI GANTC 2 cut(s) 260, 302
HphI GGTGA 1 cut(s) 266
Hpy188I TCNGA 2 cut(s) 35, 183
Hpy188III TCNNGA 2 cut(s) 287, 289
HpyCH4IV ACGT 2 cut(s) 128, 384
HpyCH4V TGCA 2 cut(s) 67, 72
HpyF10VI GCNNNNNNNGC 1 cut(s) 78
HpyF3I CTNAG 1 cut(s) 408
HpySE526I ACGT 2 cut(s) 128, 384
Kzo9I GATC 2 cut(s) 178, 319
LpnPI CCDG 2 cut(s) 151, 201
LweI GCATC 1 cut(s) 54
MaeI CTAG 2 cut(s) 96, 264
MaeII ACGT 2 cut(s) 128, 384
MaeIII GTNAC 1 cut(s) 133
MalI GATC 2 cut(s) 180, 321
MboI GATC 2 cut(s) 178, 319
MboII GAAGA 7 cut(s) 28, 208, 220, 223, 241, 257, 269
MluCI AATT 3 cut(s) 50, 110, 359
Mph1103I ATGCAT 1 cut(s) 69
MroXI GAANNNNTTC 1 cut(s) 282
MseI TTAA 3 cut(s) 147, 204, 240
MspCI CTTAAG 1 cut(s) 203
MwoI GCNNNNNNNGC 1 cut(s) 78
NdeII GATC 2 cut(s) 178, 319
NmuCI GTSAC 1 cut(s) 133
NsiI ATGCAT 1 cut(s) 69
PaeR7I CTCGAG 1 cut(s) 287
PdmI GAANNNNTTC 1 cut(s) 282
PfeI GAWTC 2 cut(s) 260, 302
PflFI GACNNNGTC 1 cut(s) 29
Psp1406I AACGTT 1 cut(s) 128
PsyI GACNNNGTC 1 cut(s) 29
SaqAI TTAA 3 cut(s) 147, 204, 240
Sau3AI GATC 2 cut(s) 178, 319
SetI ASST 5 cut(s) 131, 159, 204, 256, 387
SfaNI GCATC 1 cut(s) 54
Sfr274I CTCGAG 1 cut(s) 287
SlaI CTCGAG 1 cut(s) 287
SmlI CTYRAG 2 cut(s) 203, 287
SmoI CTYRAG 2 cut(s) 203, 287
Sse9I AATT 3 cut(s) 50, 110, 359
SspMI CTAG 2 cut(s) 96, 264
TaiI ACGT 2 cut(s) 131, 387
TaqI TCGA 5 cut(s) 48, 62, 288, 305, 326
TasI AATT 3 cut(s) 50, 110, 359
TfiI GAWTC 2 cut(s) 260, 302
Tru1I TTAA 3 cut(s) 147, 204, 240
Tru9I TTAA 3 cut(s) 147, 204, 240
TseFI GTSAC 1 cut(s) 133
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 1 cut(s) 393
TspGWI ACGGA 1 cut(s) 152
Tth111I GACNNNGTC 1 cut(s) 29
Vha464I CTTAAG 1 cut(s) 203
XapI RAATTY 3 cut(s) 50, 110, 359
XhoI CTCGAG 1 cut(s) 287
XmnI GAANNNNTTC 1 cut(s) 282
XspI CTAG 2 cut(s) 96, 264
Zsp2I ATGCAT 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.