Rw4G019550

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0011957
Physical Location & Seq
Reverse (-)
42936265 .. 42938443
2179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G019550.1

Sequence Viewer

Length: 423 bp
ATGAGGTGTCTAGTCTTGTATTGTGTTACGTACAATAATAAGAAGAAAAATGAGCAAGAAACCAAAGTAGTCGTTGCAGAATTCAAAGTCTCAATGCACTGCAATGCATGTGAAAGAATAGTTGCCAAAACACTATTGAAAATCAAAGGGGTGGAGCAGTTTGCAACAGACAGGAACAACCACAAGGTTGTGGTAACGGGGAAGATTGATCCAGAAAAGATTTTGAAGAAACTAAGGAAAAAGACGGGGAAGAAAGTAGAGATTATTGATGACAAGGAAGAAAAACCAAAAGACGCGTCTGACGATGGAAATTGGGCAAGACCATTAATTGTTCATCCACTCATGTTTGATTGCTGCAAGGAGAGCGATCAGTTACTTATGATGTTTAGTGATGAGAATCCAAATGCTTGTTCCATAATGTAA

Protein Analysis

140

Amino Acids

16.07

Weight (kDa)

8.79

Isoelectric Point (pI)

21.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 28 - 85 1.4e-13 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011957)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G21490
fragaria_vesca FvH4_3g29750 FvH4_4g16960 FvH4_4g16960 FvH4_4g16960 FvH4_4g16960
malus_domestica MD16G1177100.v1.1
prunus_persica Prupe.1G144000_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0420551
rosa_laevigata RLG00000007752
rosa_multiflora Rmu_sc0002248.1_g000057
rosa_roxburghii Rroxscaffold_5G00363170
rosa_rugosa Rorug04G0164800 Rorug04G0164900 Rorug04G0165000
rosa_samantha Rh4AG226400 Rh4CG241200 Rh4DG224700
rosa_wichuraiana Rw4G019550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 296
AclWI GGATC 1 cut(s) 203
AcsI RAATTY 1 cut(s) 80
AfaI GTAC 1 cut(s) 32
AflIII ACRYGT 1 cut(s) 294
AgsI TTSAA 3 cut(s) 85, 139, 226
AjuI GAANNNNNNNTTGG 1 cut(s) 394
Alw26I GTCTC 1 cut(s) 94
AlwI GGATC 1 cut(s) 203
ApeKI GCWGC 1 cut(s) 354
ApoI RAATTY 1 cut(s) 80
ArsI GACNNNNNNTTYG 2 cut(s) 281, 313
AseI ATTAAT 1 cut(s) 326
BbvI GCAGC 1 cut(s) 341
BccI CCATC 1 cut(s) 299
BcoDI GTCTC 1 cut(s) 94
BfaI CTAG 1 cut(s) 11
BisI GCNGC 1 cut(s) 355
BlsI GCNGC 1 cut(s) 356
BsaAI YACGTR 1 cut(s) 30
BsaBI GATNNNNATC 1 cut(s) 396
Bse3DI GCAATG 1 cut(s) 109
Bse8I GATNNNNATC 1 cut(s) 396
BseGI GGATG 1 cut(s) 334
BseJI GATNNNNATC 1 cut(s) 396
BseMI GCAATG 1 cut(s) 109
BseXI GCAGC 1 cut(s) 341
Bsh1236I CGCG 1 cut(s) 296
BsmAI GTCTC 1 cut(s) 94
Bsp143I GATC 2 cut(s) 208, 367
BspFNI CGCG 1 cut(s) 296
BspPI GGATC 1 cut(s) 203
BsrDI GCAATG 1 cut(s) 109
BssMI GATC 2 cut(s) 208, 367
BstBAI YACGTR 1 cut(s) 30
BstDEI CTNAG 1 cut(s) 233
BstF5I GGATG 1 cut(s) 334
BstFNI CGCG 1 cut(s) 296
BstKTI GATC 2 cut(s) 211, 370
BstMAI GTCTC 1 cut(s) 94
BstMBI GATC 2 cut(s) 208, 367
BstMWI GCNNNNNNNGC 1 cut(s) 363
BstNSI RCATGY 1 cut(s) 111
BstSNI TACGTA 1 cut(s) 30
BstUI CGCG 1 cut(s) 296
BstV1I GCAGC 1 cut(s) 341
BtsCI GGATG 1 cut(s) 334
BtsI GCAGTG 1 cut(s) 97
BtsIMutI CAGTG 1 cut(s) 97
CseI GACGC 2 cut(s) 285, 302
Csp6I GTAC 1 cut(s) 31
CviAII CATG 2 cut(s) 108, 343
CviQI GTAC 1 cut(s) 31
DdeI CTNAG 1 cut(s) 233
DpnI GATC 2 cut(s) 210, 369
DpnII GATC 2 cut(s) 208, 367
Eco105I TACGTA 1 cut(s) 30
EcoRI GAATTC 1 cut(s) 80
EcoT22I ATGCAT 1 cut(s) 109
FaeI CATG 2 cut(s) 111, 346
FaiI YATR 4 cut(s) 109, 344, 380, 416
FatI CATG 2 cut(s) 107, 342
Fnu4HI GCNGC 1 cut(s) 355
FokI GGATG 1 cut(s) 321
Fsp4HI GCNGC 1 cut(s) 355
FspBI CTAG 1 cut(s) 11
GluI GCNGC 1 cut(s) 355
HgaI GACGC 2 cut(s) 285, 302
Hin1II CATG 2 cut(s) 111, 346
HinfI GANTC 1 cut(s) 397
Hpy188I TCNGA 1 cut(s) 301
Hpy188III TCNNGA 1 cut(s) 212
HpyCH4IV ACGT 1 cut(s) 29
HpyCH4V TGCA 6 cut(s) 77, 97, 102, 107, 164, 357
HpyF10VI GCNNNNNNNGC 1 cut(s) 363
HpyF3I CTNAG 1 cut(s) 233
HpySE526I ACGT 1 cut(s) 29
Hsp92II CATG 2 cut(s) 111, 346
Kzo9I GATC 2 cut(s) 208, 367
LmnI GCTCC 1 cut(s) 154
LpnPI CCDG 2 cut(s) 157, 225
Lsp1109I GCAGC 1 cut(s) 341
MaeI CTAG 1 cut(s) 11
MaeII ACGT 1 cut(s) 29
MaeIII GTNAC 3 cut(s) 25, 193, 372
MalI GATC 2 cut(s) 210, 369
MboI GATC 2 cut(s) 208, 367
MboII GAAGA 5 cut(s) 55, 214, 238, 262, 290
MluCI AATT 3 cut(s) 80, 310, 327
MluI ACGCGT 1 cut(s) 294
Mph1103I ATGCAT 1 cut(s) 109
MseI TTAA 1 cut(s) 326
MslI CAYNNNNRTG 1 cut(s) 102
MvnI CGCG 1 cut(s) 296
MwoI GCNNNNNNNGC 1 cut(s) 363
NdeII GATC 2 cut(s) 208, 367
NlaIII CATG 2 cut(s) 111, 346
NsiI ATGCAT 1 cut(s) 109
NspI RCATGY 1 cut(s) 111
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PfeI GAWTC 1 cut(s) 397
PkrI GCNGC 1 cut(s) 356
Ppu21I YACGTR 1 cut(s) 30
PshBI ATTAAT 1 cut(s) 326
RsaI GTAC 1 cut(s) 32
RsaNI GTAC 1 cut(s) 31
RseI CAYNNNNRTG 1 cut(s) 102
SaqAI TTAA 1 cut(s) 326
SatI GCNGC 1 cut(s) 355
Sau3AI GATC 2 cut(s) 208, 367
SetI ASST 3 cut(s) 8, 32, 189
SmiMI CAYNNNNRTG 1 cut(s) 102
SnaBI TACGTA 1 cut(s) 30
Sse9I AATT 3 cut(s) 80, 310, 327
SspMI CTAG 1 cut(s) 11
TaiI ACGT 1 cut(s) 32
TasI AATT 3 cut(s) 80, 310, 327
TfiI GAWTC 1 cut(s) 397
Tru1I TTAA 1 cut(s) 326
Tru9I TTAA 1 cut(s) 326
TscAI CASTG 1 cut(s) 104
TseI GCWGC 1 cut(s) 354
TspDTI ATGAA 1 cut(s) 323
TspRI CASTG 1 cut(s) 104
VspI ATTAAT 1 cut(s) 326
XapI RAATTY 1 cut(s) 80
XceI RCATGY 1 cut(s) 111
XspI CTAG 1 cut(s) 11
Zsp2I ATGCAT 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.