RLG00000007752

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0011957
Physical Location & Seq
Forward (+)
20654988 .. 20656171
1184 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007752

Sequence Viewer

Length: 435 bp
ATGGAGAGCTTATTATGTAAGCGATTATCTTTCTTAGAGGAGGGTATCCTCGACGGGGAACGTTGTTATCAAGAGATGGTAGTCGTTGCAGAATTCAAAGTCTCAATGCACTGCAATGCATGTGAAAGAATAGTTGCCAAAACCCTATTGAAAATCAAAGGGGTGGAGCAGTTTGCAACAGACATGAACAACCACAAGGTTGTGGTAACGGGGAAGATTGATCCAGAAAAGATTTTGAAGAAACTAAGGAAAAAGACGGGGAAGAAAGTAGAGATTATTGATGACAAGGAAGAAAAACCAAAAGACGCGTCTGATGAAGGAAATTGGGCAAGACCATTAATTGTTCATCCACTCATGTTTGATTGCTGCAAGGAAAGCGATCAGTTACTTATGATGTTTAGTGATGAGAATCCAAATGCTTGTTCCATAATGTAA

Protein Analysis

145

Amino Acids

16.45

Weight (kDa)

5.97

Isoelectric Point (pI)

25.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 32 - 89 4.5e-14 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011957)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G21490
fragaria_vesca FvH4_3g29750 FvH4_4g16960 FvH4_4g16960 FvH4_4g16960 FvH4_4g16960
malus_domestica MD16G1177100.v1.1
prunus_persica Prupe.1G144000_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0420551
rosa_laevigata RLG00000007752
rosa_multiflora Rmu_sc0002248.1_g000057
rosa_roxburghii Rroxscaffold_5G00363170
rosa_rugosa Rorug04G0164800 Rorug04G0164900 Rorug04G0165000
rosa_samantha Rh4AG226400 Rh4CG241200 Rh4DG224700
rosa_wichuraiana Rw4G019550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 308
AclI AACGTT 1 cut(s) 61
AclWI GGATC 1 cut(s) 215
AcsI RAATTY 1 cut(s) 92
AfiI CCNNNNNNNGG 1 cut(s) 55
AflIII ACRYGT 1 cut(s) 306
AgsI TTSAA 3 cut(s) 97, 151, 238
AjuI GAANNNNNNNTTGG 1 cut(s) 406
AluBI AGCT 1 cut(s) 9
AluI AGCT 1 cut(s) 9
Alw26I GTCTC 1 cut(s) 106
AlwI GGATC 1 cut(s) 215
ApeKI GCWGC 1 cut(s) 366
ApoI RAATTY 1 cut(s) 92
ArsI GACNNNNNNTTYG 2 cut(s) 293, 325
AseI ATTAAT 1 cut(s) 338
BbvI GCAGC 1 cut(s) 353
BccI CCATC 1 cut(s) 70
BciVI GTATCC 1 cut(s) 56
BcoDI GTCTC 1 cut(s) 106
BfuI GTATCC 1 cut(s) 56
BisI GCNGC 1 cut(s) 367
BlsI GCNGC 1 cut(s) 368
BsaBI GATNNNNATC 1 cut(s) 408
Bsc4I CCNNNNNNNGG 1 cut(s) 55
Bse3DI GCAATG 1 cut(s) 121
Bse8I GATNNNNATC 1 cut(s) 408
BseGI GGATG 1 cut(s) 346
BseJI GATNNNNATC 1 cut(s) 408
BseLI CCNNNNNNNGG 1 cut(s) 55
BseMI GCAATG 1 cut(s) 121
BseRI GAGGAG 1 cut(s) 53
BseXI GCAGC 1 cut(s) 353
Bsh1236I CGCG 1 cut(s) 308
BslI CCNNNNNNNGG 1 cut(s) 55
BsmAI GTCTC 1 cut(s) 106
Bsp143I GATC 2 cut(s) 220, 379
BspFNI CGCG 1 cut(s) 308
BspPI GGATC 1 cut(s) 215
BsrDI GCAATG 1 cut(s) 121
BssMI GATC 2 cut(s) 220, 379
BstDEI CTNAG 2 cut(s) 34, 245
BstF5I GGATG 1 cut(s) 346
BstFNI CGCG 1 cut(s) 308
BstKTI GATC 2 cut(s) 223, 382
BstMAI GTCTC 1 cut(s) 106
BstMBI GATC 2 cut(s) 220, 379
BstMWI GCNNNNNNNGC 1 cut(s) 375
BstNSI RCATGY 1 cut(s) 123
BstUI CGCG 1 cut(s) 308
BstV1I GCAGC 1 cut(s) 353
BsuI GTATCC 1 cut(s) 56
BtsCI GGATG 1 cut(s) 346
BtsI GCAGTG 1 cut(s) 109
BtsIMutI CAGTG 1 cut(s) 109
CseI GACGC 2 cut(s) 297, 314
CviAII CATG 3 cut(s) 120, 184, 355
CviJI RGCY 1 cut(s) 9
CviKI_1 RGCY 1 cut(s) 9
DdeI CTNAG 2 cut(s) 34, 245
DpnI GATC 2 cut(s) 222, 381
DpnII GATC 2 cut(s) 220, 379
EcoRI GAATTC 1 cut(s) 92
EcoT22I ATGCAT 1 cut(s) 121
FaeI CATG 3 cut(s) 123, 187, 358
FaiI YATR 6 cut(s) 16, 121, 185, 356, 392, 428
FatI CATG 3 cut(s) 119, 183, 354
Fnu4HI GCNGC 1 cut(s) 367
FokI GGATG 1 cut(s) 333
Fsp4HI GCNGC 1 cut(s) 367
GluI GCNGC 1 cut(s) 367
HgaI GACGC 2 cut(s) 297, 314
Hin1II CATG 3 cut(s) 123, 187, 358
HinfI GANTC 1 cut(s) 409
Hpy188I TCNGA 1 cut(s) 313
Hpy188III TCNNGA 2 cut(s) 71, 224
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 1 cut(s) 311
HpyCH4IV ACGT 1 cut(s) 61
HpyCH4V TGCA 6 cut(s) 89, 109, 114, 119, 176, 369
HpyF10VI GCNNNNNNNGC 1 cut(s) 375
HpyF3I CTNAG 2 cut(s) 34, 245
HpySE526I ACGT 1 cut(s) 61
Hsp92II CATG 3 cut(s) 123, 187, 358
Kzo9I GATC 2 cut(s) 220, 379
LmnI GCTCC 1 cut(s) 166
LpnPI CCDG 1 cut(s) 237
Lsp1109I GCAGC 1 cut(s) 353
MaeII ACGT 1 cut(s) 61
MaeIII GTNAC 2 cut(s) 205, 384
MalI GATC 2 cut(s) 222, 381
MboI GATC 2 cut(s) 220, 379
MboII GAAGA 4 cut(s) 226, 250, 274, 302
MluCI AATT 3 cut(s) 92, 322, 339
MluI ACGCGT 1 cut(s) 306
MnlI CCTC 3 cut(s) 31, 34, 59
Mph1103I ATGCAT 1 cut(s) 121
MseI TTAA 1 cut(s) 338
MslI CAYNNNNRTG 1 cut(s) 114
MvnI CGCG 1 cut(s) 308
MwoI GCNNNNNNNGC 1 cut(s) 375
NdeII GATC 2 cut(s) 220, 379
NlaIII CATG 3 cut(s) 123, 187, 358
NsiI ATGCAT 1 cut(s) 121
NspI RCATGY 1 cut(s) 123
PfeI GAWTC 1 cut(s) 409
PkrI GCNGC 1 cut(s) 368
PshBI ATTAAT 1 cut(s) 338
Psp1406I AACGTT 1 cut(s) 61
RseI CAYNNNNRTG 1 cut(s) 114
SaqAI TTAA 1 cut(s) 338
SatI GCNGC 1 cut(s) 367
Sau3AI GATC 2 cut(s) 220, 379
SetI ASST 3 cut(s) 11, 64, 201
SmiMI CAYNNNNRTG 1 cut(s) 114
Sse9I AATT 3 cut(s) 92, 322, 339
TaiI ACGT 1 cut(s) 64
TaqI TCGA 1 cut(s) 51
TasI AATT 3 cut(s) 92, 322, 339
TfiI GAWTC 1 cut(s) 409
Tru1I TTAA 1 cut(s) 338
Tru9I TTAA 1 cut(s) 338
TscAI CASTG 1 cut(s) 116
TseI GCWGC 1 cut(s) 366
TspDTI ATGAA 3 cut(s) 200, 330, 335
TspRI CASTG 1 cut(s) 116
VspI ATTAAT 1 cut(s) 338
XapI RAATTY 1 cut(s) 92
XceI RCATGY 1 cut(s) 123
Zsp2I ATGCAT 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.