AT3G21650

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0006454
Physical Location & Seq
Reverse (-)
7621569 .. 7624133
2565 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G21650.1

Sequence Viewer

Length: 1641 bp
ATGATCAAACAGATATTTGGGAAATTACCTAGAAAGCCTTCCAAATCATTGCAGAATGACTCCAATGGTGAAGGAGGTGTTAATAATTCTTATTATGCTTCGAATTCGAGTACTACTAGTATCTCTAAACCTTCTTCAACATCTTCGAAATCATCATCAGCCTCGGGTTCGCGTGTAGCTAATGGAACTCTTGCTCCTAACTCAATGAGCTCTAATAGGAATACTAATCAAGGGAAGAAACCATTGGGAGGAGATGCTGTTGTACAAGCTGGACCGTTTCCGTCTTCTGGTGGGGTTTATGAGGCTTTGCCTAGCTTTAGAGATGTTCCTATATCGGAGAAACCGAATCTCTTTATCGGGAAGTTGAGTATGTGCTGTGTTGTTTTTGACTTTAGTGATCCGTCTAAGAACCTTAAGGAGAAAGAGATTAAGAGGCAGACATTGCTTGAGCTTGTTGATTATGTTGCATCAGTTGGTTTTAAGTTTAACGATGTTTCGATGCAAGAGTTAACGAAGATGGTAGCGGTTAATCTGTTTAGAACTTTTCCTTCTGCGAATCACGAGAGTAAAATTCTTGAAATACATGATATGGATGATGAAGAACCTTCTTTGGAGCCAGCTTGGCCTCATGTTCAAGTTGTGTATGAGATTCTTCTCAGATTCGTGGCTTCTCCCATGACTGATGCAAAGCTTGCCAAGAGATATATTGACCATTCTTTTGTCTTGAAGCTCTTAGACTTGTTTGATTCTGAAGATCAAAGAGAGAGGGAATATCTAAAAACTATTCTGCATCGGGTGTACGGGAAGTTCATGGTGCATCGACCTTACATCAGAAAGGCGATAAACAATATCTTCTACAGATTCATATCCGAGACTGAAAAGCATAATGGCATTGCGGAGTTGCTAGAGATTCTTGGAAGTATAATTAATGGTTTTGCTTTGCCTTTAAAAGAAGAGCACAAGCTCTTCCTTTTGCGAGCCTTGATTCCTCTCCACAAGCCTAAATGTTCATCAGTCTATCACCAACAGCTTTCGTATTGCATTGTTCAGTTTGTAGAAAAGGACTTCAAGCTCGCTGATACCGTTATTAGAGGTCTTTTAAAATATTGGCCTGTGACTAACAGCTCAAAGGAAGTTATGTTTCTTGGAGAGTTAGAAGAAGTCTTGGAAGCAACTCAAGCCGCTGAGTTTCAACGTTGTATGGTTCCATTATCCCGACAAATTGCTCGATGCCTCAACAGTTCACATTTCCAGGTTGCTGAAAGAGCATTGTTTCTATGGAACAACGATCACATAAGAAACCTGATCACTCAGAACCATAAAGTGATAATGCCTATAGTCTTCCCAGCTCTTGAGAGAAACACGCGTGGACATTGGAACCAAGCAGTTCAAAGTCTGACTATAAACGTGAGGAAAGTATTATGCGAGATTGACCAAGTTCTTTTCGACGAGTGTTTAGCCAAATTCCAAGTAGAAGAAGTGAATAAAACAGAGGTTAAAGCGAAACGGGAAAGGACATGGCAACGGTTAGAAGATTTAGCTACTTCAAAGACCGTTGTAACCAACGAGGCAGTACTGGTTCCAAGATTTGTGTCCTCAGTCAATCTTACTACAAGCAGCTCTGAGTCCACAGGGTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000096 GO:0000902 GO:0000904 GO:0002682 GO:0002683 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006082 GO:0006139 GO:0006520 GO:0006534 GO:0006555 GO:0006575 GO:0006725 GO:0006732 GO:0006790 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0009058 GO:0009066 GO:0009069 GO:0009116 GO:0009119 GO:0009653 GO:0009759 GO:0009888 GO:0009966 GO:0009968 GO:0009987 GO:0010026 GO:0010090 GO:0010646 GO:0010648 GO:0016043 GO:0016143 GO:0016144 GO:0017144 GO:0018130 GO:0019222 GO:0019438 GO:0019748 GO:0019752 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0023051 GO:0023057 GO:0030154 GO:0031323 GO:0031347 GO:0031348 GO:0032502 GO:0032989 GO:0033353 GO:0034641 GO:0042278 GO:0042343 GO:0042430 GO:0042435 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0045088 GO:0045824 GO:0046128 GO:0046439 GO:0046483 GO:0046498 GO:0046500 GO:0048468 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048856 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051186 GO:0055086 GO:0065007 GO:0071704 GO:0071840 GO:0072521 GO:0080134 GO:0080183 GO:0090342 GO:0090558 GO:0090626 GO:0097159 GO:1900457 GO:1900458 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901657 GO:1901659 GO:2000377
KEGG Pathways
Metabolic & Signaling
ko03015 ko04071 ko04113 ko04114 ko04151 ko04152 ko04261 ko04728 ko05165 map03015 map04071 map04113 map04114 map04151 map04152 map04261 map04728 map05165
Pfam Domains
Protein Families
B56

Protein Analysis

546

Amino Acids

61.73

Weight (kDa)

8.69

Isoelectric Point (pI)

39.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B56 PF01603 103 - 514 8.4e-187 Protein phosphatase 2A regulatory B subunit (B56 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 172, 1366
AciI CCGC 3 cut(s) 524, 896, 1182
AclI AACGTT 1 cut(s) 1195
AclWI GGATC 1 cut(s) 392
AcsI RAATTY 3 cut(s) 103, 570, 1463
AcuI CTGAAG 1 cut(s) 771
AfaI GTAC 4 cut(s) 112, 264, 800, 1575
AfiI CCNNNNNNNGG 2 cut(s) 248, 287
AflII CTTAAG 1 cut(s) 413
AflIII ACRYGT 1 cut(s) 1364
AgsI TTSAA 8 cut(s) 138, 578, 635, 727, 1069, 1193, 1391, 1548
AhlI ACTAGT 1 cut(s) 116
AjnI CCWGG 1 cut(s) 1251
AjuI GAANNNNNNNTTGG 2 cut(s) 227, 259
AloI GAACNNNNNNTCC 2 cut(s) 178, 210
Alw21I GWGCWC 2 cut(s) 212, 960
Alw26I GTCTC 1 cut(s) 866
AlwI GGATC 1 cut(s) 392
Ama87I CYCGRG 1 cut(s) 163
AoxI GGCC 2 cut(s) 623, 1109
ApeKI GCWGC 1 cut(s) 1617
ApoI RAATTY 3 cut(s) 103, 570, 1463
ArsI GACNNNNNNTTYG 2 cut(s) 701, 733
AseI ATTAAT 1 cut(s) 927
Asp700I GAANNNNTTC 1 cut(s) 37
AspS9I GGNCC 1 cut(s) 272
AsuHPI GGTGA 2 cut(s) 80, 1013
AsuII TTCGAA 2 cut(s) 101, 146
AvaI CYCGRG 1 cut(s) 163
AvaII GGWCC 1 cut(s) 272
BanII GRGCYC 1 cut(s) 212
BauI CACGAG 1 cut(s) 560
BbsI GAAGAC 2 cut(s) 276, 1333
Bbv12I GWGCWC 2 cut(s) 212, 960
BbvI GCAGC 1 cut(s) 1629
BccI CCATC 1 cut(s) 511
BciT130I CCWGG 1 cut(s) 1253
BclI TGATCA 2 cut(s) 3, 1305
BcoDI GTCTC 1 cut(s) 866
BcuI ACTAGT 1 cut(s) 116
BfaI CTAG 4 cut(s) 30, 117, 312, 905
BfmI CTRYAG 2 cut(s) 856, 1335
BfrI CTTAAG 1 cut(s) 413
BglI GCCNNNNNGGC 1 cut(s) 622
BisI GCNGC 2 cut(s) 1182, 1618
BlsI GCNGC 2 cut(s) 1183, 1619
BmcAI AGTACT 2 cut(s) 112, 1575
Bme1390I CCNGG 1 cut(s) 1253
Bme18I GGWCC 1 cut(s) 272
BmeT110I CYCGRG 1 cut(s) 163
BmgT120I GGNCC 1 cut(s) 272
BmiI GGNNCC 4 cut(s) 615, 1206, 1379, 1581
BmrFI CCNGG 1 cut(s) 1253
BmsI GCATC 7 cut(s) 244, 476, 489, 673, 799, 826, 1220
BpiI GAAGAC 2 cut(s) 276, 1333
Bpu14I TTCGAA 2 cut(s) 101, 146
BpuEI CTTGAG 3 cut(s) 467, 1161, 1373
BsaBI GATNNNNATC 1 cut(s) 865
BsaJI CCNNGG 1 cut(s) 162
BsaXI ACNNNNNCTCC 4 cut(s) 178, 208, 243, 273
Bsc4I CCNNNNNNNGG 2 cut(s) 248, 287
Bse1I ACTGG 1 cut(s) 1581
Bse3DI GCAATG 3 cut(s) 47, 440, 891
Bse8I GATNNNNATC 1 cut(s) 865
BseBI CCWGG 1 cut(s) 1253
BseDI CCNNGG 1 cut(s) 162
BseGI GGATG 1 cut(s) 598
BseJI GATNNNNATC 1 cut(s) 865
BseLI CCNNNNNNNGG 2 cut(s) 248, 287
BseMI GCAATG 3 cut(s) 47, 440, 891
BseMII CTCAG 5 cut(s) 670, 1176, 1325, 1611, 1614
BseNI ACTGG 1 cut(s) 1581
BseRI GAGGAG 1 cut(s) 264
BseXI GCAGC 1 cut(s) 1629
BseYI CCCAGC 1 cut(s) 1345
Bsh1236I CGCG 2 cut(s) 172, 1366
BshFI GGCC 2 cut(s) 625, 1111
BsiHKAI GWGCWC 2 cut(s) 212, 960
BsiHKCI CYCGRG 1 cut(s) 163
BslI CCNNNNNNNGG 2 cut(s) 248, 287
BsmAI GTCTC 1 cut(s) 866
BsnI GGCC 2 cut(s) 625, 1111
BsoBI CYCGRG 1 cut(s) 163
Bsp119I TTCGAA 2 cut(s) 101, 146
Bsp1286I GDGCHC 2 cut(s) 212, 960
Bsp1407I TGTACA 1 cut(s) 262
Bsp143I GATC 5 cut(s) 3, 397, 754, 1288, 1305
BspACI CCGC 3 cut(s) 524, 896, 1182
BspANI GGCC 2 cut(s) 625, 1111
BspCNI CTCAG 5 cut(s) 669, 1177, 1324, 1610, 1615
BspFNI CGCG 2 cut(s) 172, 1366
BspLI GGNNCC 4 cut(s) 615, 1206, 1379, 1581
BspPI GGATC 1 cut(s) 392
BspQI GCTCTTC 2 cut(s) 948, 971
BspT104I TTCGAA 2 cut(s) 101, 146
BspTI CTTAAG 1 cut(s) 413
BsrDI GCAATG 3 cut(s) 47, 440, 891
BsrGI TGTACA 1 cut(s) 262
BsrI ACTGG 1 cut(s) 1581
BssECI CCNNGG 1 cut(s) 162
BssMI GATC 5 cut(s) 3, 397, 754, 1288, 1305
BssSI CACGAG 1 cut(s) 560
Bst2BI CACGAG 1 cut(s) 560
Bst2UI CCWGG 1 cut(s) 1253
Bst4CI ACNGT 5 cut(s) 276, 1084, 1241, 1527, 1555
Bst6I CTCTTC 2 cut(s) 948, 971
BstAFI CTTAAG 1 cut(s) 413
BstAPI GCANNNNNTGC 2 cut(s) 442, 692
BstAUI TGTACA 1 cut(s) 262
BstBI TTCGAA 2 cut(s) 101, 146
BstC8I GCNNGC 4 cut(s) 618, 693, 978, 1074
BstDEI CTNAG 7 cut(s) 405, 656, 733, 1185, 1311, 1597, 1623
BstF5I GGATG 1 cut(s) 598
BstFNI CGCG 2 cut(s) 172, 1366
BstKTI GATC 5 cut(s) 6, 400, 757, 1291, 1308
BstMAI GTCTC 1 cut(s) 866
BstMBI GATC 5 cut(s) 3, 397, 754, 1288, 1305
BstMWI GCNNNNNNNGC 5 cut(s) 442, 622, 692, 1178, 1265
BstNI CCWGG 1 cut(s) 1253
BstSCI CCNGG 1 cut(s) 1251
BstSFI CTRYAG 2 cut(s) 856, 1335
BstUI CGCG 2 cut(s) 172, 1366
BstV1I GCAGC 1 cut(s) 1629
BstV2I GAAGAC 2 cut(s) 276, 1333
BsuRI GGCC 2 cut(s) 625, 1111
BtsCI GGATG 1 cut(s) 598
Cac8I GCNNGC 4 cut(s) 618, 693, 978, 1074
Cfr13I GGNCC 1 cut(s) 272
Csp6I GTAC 4 cut(s) 111, 263, 799, 1574
CviAII CATG 5 cut(s) 584, 629, 676, 811, 1518
CviQI GTAC 4 cut(s) 111, 263, 799, 1574
DdeI CTNAG 7 cut(s) 405, 656, 733, 1185, 1311, 1597, 1623
DpnI GATC 5 cut(s) 5, 399, 756, 1290, 1307
DpnII GATC 5 cut(s) 3, 397, 754, 1288, 1305
DraI TTTAAA 2 cut(s) 948, 1101
Eam1104I CTCTTC 2 cut(s) 948, 971
EarI CTCTTC 2 cut(s) 948, 971
Ecl136II GAGCTC 1 cut(s) 210
Eco24I GRGCYC 1 cut(s) 212
Eco47I GGWCC 1 cut(s) 272
Eco53kI GAGCTC 1 cut(s) 210
Eco57I CTGAAG 1 cut(s) 771
Eco88I CYCGRG 1 cut(s) 163
EcoICRI GAGCTC 1 cut(s) 210
EcoRI GAATTC 1 cut(s) 103
EcoRII CCWGG 1 cut(s) 1251
EcoT38I GRGCYC 1 cut(s) 212
FaeI CATG 5 cut(s) 587, 632, 679, 814, 1521
FatI CATG 5 cut(s) 583, 628, 675, 810, 1517
FbaI TGATCA 2 cut(s) 3, 1305
Fnu4HI GCNGC 2 cut(s) 1182, 1618
FokI GGATG 1 cut(s) 605
FriOI GRGCYC 1 cut(s) 212
Fsp4HI GCNGC 2 cut(s) 1182, 1618
FspBI CTAG 4 cut(s) 30, 117, 312, 905
GluI GCNGC 2 cut(s) 1182, 1618
GsaI CCCAGC 1 cut(s) 1349
HaeIII GGCC 2 cut(s) 625, 1111
Hin1II CATG 5 cut(s) 587, 632, 679, 814, 1521
HincII GTYRAC 1 cut(s) 510
HindII GTYRAC 1 cut(s) 510
HindIII AAGCTT 1 cut(s) 689
HpaI GTTAAC 1 cut(s) 510
HphI GGTGA 2 cut(s) 80, 1013
Hpy166II GTNNAC 5 cut(s) 510, 799, 1244, 1370, 1629
Hpy188I TCNGA 8 cut(s) 337, 659, 751, 833, 871, 1314, 1398, 1624
Hpy188III TCNNGA 6 cut(s) 358, 560, 575, 724, 1215, 1352
Hpy8I GTNNAC 5 cut(s) 510, 799, 1244, 1370, 1629
Hpy99I CGWCG 1 cut(s) 1451
HpyAV CCTTC 5 cut(s) 48, 65, 141, 558, 615
HpyCH4III ACNGT 5 cut(s) 276, 1084, 1241, 1527, 1555
HpyCH4IV ACGT 2 cut(s) 1195, 1407
HpyCH4V TGCA 7 cut(s) 52, 467, 502, 686, 790, 817, 1041
HpyF10VI GCNNNNNNNGC 5 cut(s) 442, 622, 692, 1178, 1265
HpyF3I CTNAG 7 cut(s) 405, 656, 733, 1185, 1311, 1597, 1623
HpySE526I ACGT 2 cut(s) 1195, 1407
Hsp92II CATG 5 cut(s) 587, 632, 679, 814, 1521
Ksp22I TGATCA 2 cut(s) 3, 1305
KspAI GTTAAC 1 cut(s) 510
Kzo9I GATC 5 cut(s) 3, 397, 754, 1288, 1305
LguI GCTCTTC 2 cut(s) 948, 971
LmnI GCTCC 2 cut(s) 199, 613
Lsp1109I GCAGC 1 cut(s) 1629
LweI GCATC 7 cut(s) 244, 476, 489, 673, 799, 826, 1220
MaeI CTAG 4 cut(s) 30, 117, 312, 905
MaeII ACGT 2 cut(s) 1195, 1407
MaeIII GTNAC 2 cut(s) 1114, 1558
MalI GATC 5 cut(s) 5, 399, 756, 1290, 1307
MboI GATC 5 cut(s) 3, 397, 754, 1288, 1305
MhlI GDGCHC 2 cut(s) 212, 960
MluCI AATT 7 cut(s) 23, 85, 103, 570, 924, 1221, 1463
MluI ACGCGT 1 cut(s) 1364
MlyI GAGTC 2 cut(s) 53, 1634
MroXI GAANNNNTTC 1 cut(s) 37
MspA1I CMGCKG 1 cut(s) 1184
MspCI CTTAAG 1 cut(s) 413
MspR9I CCNGG 1 cut(s) 1253
MvaI CCWGG 1 cut(s) 1253
MvnI CGCG 2 cut(s) 172, 1366
MwoI GCNNNNNNNGC 5 cut(s) 442, 622, 692, 1178, 1265
NdeII GATC 5 cut(s) 3, 397, 754, 1288, 1305
NlaIII CATG 5 cut(s) 587, 632, 679, 814, 1521
NlaIV GGNNCC 4 cut(s) 615, 1206, 1379, 1581
NmuCI GTSAC 1 cut(s) 1114
NspV TTCGAA 2 cut(s) 101, 146
PciSI GCTCTTC 2 cut(s) 948, 971
PcsI WCGNNNNNNNCGW 2 cut(s) 341, 1080
PdmI GAANNNNTTC 1 cut(s) 37
PfeI GAWTC 8 cut(s) 346, 556, 649, 660, 746, 861, 910, 985
PkrI GCNGC 2 cut(s) 1183, 1619
PleI GAGTC 2 cut(s) 53, 1633
PpsI GAGTC 2 cut(s) 53, 1633
PshBI ATTAAT 1 cut(s) 927
Psp124BI GAGCTC 1 cut(s) 212
Psp1406I AACGTT 1 cut(s) 1195
Psp6I CCWGG 1 cut(s) 1251
PspFI CCCAGC 1 cut(s) 1345
PspGI CCWGG 1 cut(s) 1251
PspN4I GGNNCC 4 cut(s) 615, 1206, 1379, 1581
PspPI GGNCC 1 cut(s) 272
PsrI GAACNNNNNNTAC 2 cut(s) 791, 823
RsaI GTAC 4 cut(s) 112, 264, 800, 1575
RsaNI GTAC 4 cut(s) 111, 263, 799, 1574
SacI GAGCTC 1 cut(s) 212
SapI GCTCTTC 2 cut(s) 948, 971
SatI GCNGC 2 cut(s) 1182, 1618
Sau3AI GATC 5 cut(s) 3, 397, 754, 1288, 1305
Sau96I GGNCC 1 cut(s) 272
ScaI AGTACT 2 cut(s) 112, 1575
SchI GAGTC 2 cut(s) 53, 1634
ScrFI CCNGG 1 cut(s) 1253
SduI GDGCHC 2 cut(s) 212, 960
SfaNI GCATC 7 cut(s) 244, 476, 489, 673, 799, 826, 1220
SfcI CTRYAG 2 cut(s) 856, 1335
SfuI TTCGAA 2 cut(s) 101, 146
SinI GGWCC 1 cut(s) 272
SmlI CTYRAG 4 cut(s) 413, 446, 1176, 1352
SmoI CTYRAG 4 cut(s) 413, 446, 1176, 1352
SpeI ACTAGT 1 cut(s) 116
Sse9I AATT 7 cut(s) 23, 85, 103, 570, 924, 1221, 1463
SsiI CCGC 3 cut(s) 524, 896, 1182
SspI AATATT 1 cut(s) 1106
SspMI CTAG 4 cut(s) 30, 117, 312, 905
SstI GAGCTC 1 cut(s) 212
StyD4I CCNGG 1 cut(s) 1251
TaaI ACNGT 5 cut(s) 276, 1084, 1241, 1527, 1555
TaiI ACGT 2 cut(s) 1198, 1410
TaqI TCGA 7 cut(s) 101, 107, 146, 497, 820, 1228, 1446
TasI AATT 7 cut(s) 23, 85, 103, 570, 924, 1221, 1463
TatI WGTACW 3 cut(s) 110, 262, 1573
TauI GCSGC 1 cut(s) 1184
TfiI GAWTC 8 cut(s) 346, 556, 649, 660, 746, 861, 910, 985
TseFI GTSAC 1 cut(s) 1114
TseI GCWGC 1 cut(s) 1617
Tsp45I GTSAC 1 cut(s) 1114
TspDTI ATGAA 4 cut(s) 612, 799, 853, 999
TspGWI ACGGA 2 cut(s) 270, 390
Vha464I CTTAAG 1 cut(s) 413
VpaK11BI GGWCC 1 cut(s) 272
VspI ATTAAT 1 cut(s) 927
XapI RAATTY 3 cut(s) 103, 570, 1463
XmnI GAANNNNTTC 1 cut(s) 37
XspI CTAG 4 cut(s) 30, 117, 312, 905
ZrmI AGTACT 2 cut(s) 112, 1575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.