FvH4_6g42590

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0006454
Physical Location & Seq
Forward (+)
33157714 .. 33161574
3861 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g42590.t3

Sequence Viewer

Length: 1581 bp
ATGATCAAACAAATTCTGGGTAGAATACCTCGAAAACCTTCGAAATCATCGCAAAACGATGCATACAACGATGGAGGGGTTAATGGGATTGGACCCAATTCCATTAACAGCTCAAAGTCTTCGAATTCAGGTTCTGGGAATTCAAGAACCAGCAATGGGGTTCTTGCCCCACTGTCTAGTAAACCCAGTCAATCCAAGAAGCCAGGTCAAGTAGGTCCTTTGTTGTTTTCGGGTGCCTATGAGGCCCTGCCGAGTTTCAGGGATGTCCCCAGTGCAGAGAAGCAGGGTCTGTTTATCAAGAAATTGAACATGTGTTGTGTTGTGTTTGATTTCAATGATCCGTGGAAGAATGTCAAGGAGAAGGATGTAAAGAGGCAGACTTTGCTTGAGCTTGTGGATTACATTTCGTCTGTGAATTCCAAGTTCAATGAGGTGACAATGCAGGAGATTACTAAGATGGTGGCCACCAATTTGTTTCGGACTTTTCCATCCCCGAGTCATGAGAAGAAGGCTCTTGAGAGCTATGACCAGGACGAGGAGGACCAGATGTTGGAGCCTGCTTGGTCTCATCTTCAGGTTGTGTATGAGTTTCTGTTAAGATTTGTGGCTTCTCCTGAGACAGATGCCAAGCTTGCTAAAAGATATATTGATCAGGCGTTTGTTTTGAAGTTGCTTGATTTGTTTGACTCTGAGGATCAAAGAGAGAGGGAATACTTAAAAACAATCCTACACCGGGTTTATGGGAAGTTCATGGTGCATCGACCATTCATTAGGAAAGCCATCAATAATATCTTCTTTTGGTTCATATTTGAGACTCAGAAGCACAATGGGATTGCAGAAATGCTTGAAATTTTAGGCAGTATAATCAATGGTTTTGCTTTACCTTTGAAGGAAGAGCACAAGCTGTTCCTTGTCCGCGCCTTGATTCCCCTTCACAAGCCTAAATGTGTATCTATGTATCATCAGCAACTTTCCTATTGCATTACTCAGTTTGTGGAGAAAGATGGCAAGCTGTCTGATACCGTGATTAGAGGACTGTTGAAGTATTGGCCCATAACCAATAGTTCAAAGGAAGTCATGTTCCTTGGTGAATTGGAAGAAGTTCTGGAAGCTACCCAACCATCAGAATTTCAGCGGTGCATGGTCCCTCTTTTCCGTCAAATTGGTCACTGCCTCAGCAGCTCACATTTTCAGGTAGCTGAACGCGCATTGTTTTTGTGGAATAATGATCACATAAGAACTTTGATCACACAAAACCGCCAAGTGATTCTGCCAATAATTTTCCCTGCTATGGAGGAAAATGCTCGGAGTCACTGGAATCAAGCTGTTCGGAGTTTGACATTGAATGTGAGAAAGATATTCTCAGATATTGATCAAGAACTCTTTGATGAGTGCTTGGCCACATTCCAAGAAGATGAAACTAAAGAGAAGGAAACACAGGAGAAACGTGAATCAACCTGGAAGCGCTTGGAAGCTGTGGCTTCCTCCAAGTCTTTGAACAATGAGGCCGACCTTGTCTCAAGGTTTGCTTCTAATGCCATTTCTACAAGCCAAAAACCCCGGACCACTTCTGGTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000096 GO:0000902 GO:0000904 GO:0002682 GO:0002683 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006082 GO:0006139 GO:0006520 GO:0006534 GO:0006555 GO:0006575 GO:0006725 GO:0006732 GO:0006790 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0009058 GO:0009066 GO:0009069 GO:0009116 GO:0009119 GO:0009653 GO:0009759 GO:0009888 GO:0009966 GO:0009968 GO:0009987 GO:0010026 GO:0010090 GO:0010646 GO:0010648 GO:0016043 GO:0016143 GO:0016144 GO:0017144 GO:0018130 GO:0019222 GO:0019438 GO:0019748 GO:0019752 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0023051 GO:0023057 GO:0030154 GO:0031323 GO:0031347 GO:0031348 GO:0032502 GO:0032989 GO:0033353 GO:0034641 GO:0042278 GO:0042343 GO:0042430 GO:0042435 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0045088 GO:0045824 GO:0046128 GO:0046439 GO:0046483 GO:0046498 GO:0046500 GO:0048468 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048856 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051186 GO:0055086 GO:0065007 GO:0071704 GO:0071840 GO:0072521 GO:0080134 GO:0080183 GO:0090342 GO:0090558 GO:0090626 GO:0097159 GO:1900457 GO:1900458 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901657 GO:1901659 GO:2000377
KEGG Pathways
Metabolic & Signaling
ko03015 ko04071 ko04113 ko04114 ko04151 ko04152 ko04261 ko04728 ko05165 map03015 map04071 map04113 map04114 map04151 map04152 map04261 map04728 map05165
Pfam Domains
Protein Families
B56

Protein Analysis

527

Amino Acids

60.18

Weight (kDa)

8.29

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B56 PF01603 83 - 398 1.4e-147 Protein phosphatase 2A regulatory B subunit (B56 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 233
AccB7I CCANNNNNTGG 1 cut(s) 550
AccII CGCG 2 cut(s) 918, 1206
AciI CCGC 3 cut(s) 916, 1135, 1258
AclWI GGATC 2 cut(s) 332, 702
AcoI YGGCCR 2 cut(s) 462, 1398
AcsI RAATTY 6 cut(s) 12, 124, 139, 415, 849, 1127
AcuI CTGAAG 1 cut(s) 557
AfeI AGCGCT 1 cut(s) 1466
AfiI CCNNNNNNNGG 5 cut(s) 156, 535, 550, 733, 1291
AflIII ACRYGT 1 cut(s) 309
AjnI CCWGG 3 cut(s) 202, 528, 1457
AloI GAACNNNNNNTCC 2 cut(s) 1064, 1096
Alw21I GWGCWC 1 cut(s) 900
Alw26I GTCTC 4 cut(s) 570, 611, 806, 1522
AlwI GGATC 2 cut(s) 332, 702
AlwNI CAGNNNCTG 2 cut(s) 134, 289
Ama87I CYCGRG 1 cut(s) 493
Aor51HI AGCGCT 1 cut(s) 1466
AoxI GGCC 5 cut(s) 243, 462, 1049, 1398, 1506
ApeKI GCWGC 1 cut(s) 1179
ApoI RAATTY 6 cut(s) 12, 124, 139, 415, 849, 1127
Asp700I GAANNNNTTC 2 cut(s) 37, 1101
AspLEI GCGC 3 cut(s) 920, 1208, 1467
AspS9I GGNCC 7 cut(s) 92, 215, 244, 541, 1050, 1144, 1563
AsuC2I CCSGG 2 cut(s) 734, 1561
AsuHPI GGTGA 2 cut(s) 445, 1100
AsuII TTCGAA 2 cut(s) 41, 122
AvaI CYCGRG 1 cut(s) 493
AvaII GGWCC 5 cut(s) 92, 215, 541, 1144, 1563
BalI TGGCCA 2 cut(s) 464, 1400
BanI GGYRCC 1 cut(s) 233
BbsI GAAGAC 1 cut(s) 111
Bbv12I GWGCWC 1 cut(s) 900
BbvCI CCTCAGC 1 cut(s) 1175
BbvI GCAGC 1 cut(s) 1191
BccI CCATC 6 cut(s) 65, 451, 496, 788, 998, 1129
BciT130I CCWGG 3 cut(s) 204, 530, 1459
BclI TGATCA 5 cut(s) 3, 649, 1228, 1245, 1372
BcnI CCSGG 2 cut(s) 734, 1561
BcoDI GTCTC 4 cut(s) 570, 611, 806, 1522
BfaI CTAG 1 cut(s) 177
BfoI RGCGCY 1 cut(s) 1468
BglI GCCNNNNNGGC 1 cut(s) 242
BisI GCNGC 1 cut(s) 1180
BlsI GCNGC 1 cut(s) 1181
Bme1390I CCNGG 5 cut(s) 204, 530, 734, 1459, 1561
Bme18I GGWCC 5 cut(s) 92, 215, 541, 1144, 1563
BmeT110I CYCGRG 1 cut(s) 493
BmgT120I GGNCC 7 cut(s) 92, 215, 244, 541, 1050, 1144, 1563
BmiI GGNNCC 4 cut(s) 94, 235, 555, 1146
BmrFI CCNGG 5 cut(s) 204, 530, 734, 1459, 1561
BmrI ACTGGG 2 cut(s) 180, 264
BmsI GCATC 3 cut(s) 49, 613, 766
BmuI ACTGGG 2 cut(s) 180, 264
BpiI GAAGAC 1 cut(s) 111
Bpu10I CCTNAGC 1 cut(s) 1175
Bpu14I TTCGAA 2 cut(s) 41, 122
BpuEI CTTGAG 3 cut(s) 407, 536, 1504
BpuMI CCSGG 2 cut(s) 734, 1561
BsaBI GATNNNNATC 1 cut(s) 1371
BsaI GGTCTC 1 cut(s) 570
BsaJI CCNNGG 3 cut(s) 341, 1084, 1559
Bsc4I CCNNNNNNNGG 5 cut(s) 156, 535, 550, 733, 1291
Bse1I ACTGG 3 cut(s) 186, 270, 1319
Bse3DI GCAATG 1 cut(s) 160
Bse8I GATNNNNATC 1 cut(s) 1371
BseBI CCWGG 3 cut(s) 204, 530, 1459
BseDI CCNNGG 3 cut(s) 341, 1084, 1559
BseGI GGATG 3 cut(s) 268, 370, 488
BseJI GATNNNNATC 1 cut(s) 1371
BseLI CCNNNNNNNGG 5 cut(s) 156, 535, 550, 733, 1291
BseMI GCAATG 1 cut(s) 160
BseMII CTCAG 6 cut(s) 606, 681, 830, 1001, 1189, 1377
BseNI ACTGG 3 cut(s) 186, 270, 1319
BseRI GAGGAG 1 cut(s) 551
BseXI GCAGC 1 cut(s) 1191
BsgI GTGCAG 1 cut(s) 294
Bsh1236I CGCG 2 cut(s) 918, 1206
BshFI GGCC 5 cut(s) 245, 464, 1051, 1400, 1508
BshNI GGYRCC 1 cut(s) 233
BsiHKAI GWGCWC 1 cut(s) 900
BsiHKCI CYCGRG 1 cut(s) 493
BsiSI CCGG 2 cut(s) 733, 1561
BslFI GGGAC 2 cut(s) 251, 1130
BslI CCNNNNNNNGG 5 cut(s) 156, 535, 550, 733, 1291
BsmAI GTCTC 4 cut(s) 570, 611, 806, 1522
BsmFI GGGAC 2 cut(s) 251, 1130
BsnI GGCC 5 cut(s) 245, 464, 1051, 1400, 1508
Bso31I GGTCTC 1 cut(s) 570
BsoBI CYCGRG 1 cut(s) 493
Bsp119I TTCGAA 2 cut(s) 41, 122
Bsp1286I GDGCHC 1 cut(s) 900
Bsp143I GATC 7 cut(s) 3, 337, 649, 694, 1228, 1245, 1372
BspACI CCGC 3 cut(s) 916, 1135, 1258
BspANI GGCC 5 cut(s) 245, 464, 1051, 1400, 1508
BspCNI CTCAG 6 cut(s) 607, 682, 829, 1000, 1188, 1376
BspFNI CGCG 2 cut(s) 918, 1206
BspHI TCATGA 1 cut(s) 499
BspLI GGNNCC 4 cut(s) 94, 235, 555, 1146
BspPI GGATC 2 cut(s) 332, 702
BspQI GCTCTTC 1 cut(s) 888
BspT104I TTCGAA 2 cut(s) 41, 122
BspT107I GGYRCC 1 cut(s) 233
BspTNI GGTCTC 1 cut(s) 570
BsrDI GCAATG 1 cut(s) 160
BsrI ACTGG 3 cut(s) 186, 270, 1319
BssECI CCNNGG 3 cut(s) 341, 1084, 1559
BssMI GATC 7 cut(s) 3, 337, 649, 694, 1228, 1245, 1372
BssT1I CCWWGG 1 cut(s) 1084
Bst2UI CCWGG 3 cut(s) 204, 530, 1459
Bst4CI ACNGT 3 cut(s) 174, 1024, 1038
Bst6I CTCTTC 1 cut(s) 888
BstAPI GCANNNNNTGC 1 cut(s) 382
BstBI TTCGAA 2 cut(s) 41, 122
BstC8I GCNNGC 3 cut(s) 558, 633, 1010
BstDEI CTNAG 7 cut(s) 453, 615, 690, 816, 987, 1175, 1363
BstDSI CCRYGG 1 cut(s) 341
BstF5I GGATG 3 cut(s) 268, 370, 488
BstFNI CGCG 2 cut(s) 918, 1206
BstH2I RGCGCY 1 cut(s) 1468
BstHHI GCGC 3 cut(s) 920, 1208, 1467
BstKTI GATC 7 cut(s) 6, 340, 652, 697, 1231, 1248, 1375
BstMAI GTCTC 4 cut(s) 570, 611, 806, 1522
BstMBI GATC 7 cut(s) 3, 337, 649, 694, 1228, 1245, 1372
BstMWI GCNNNNNNNGC 6 cut(s) 242, 382, 632, 1179, 1205, 1535
BstNI CCWGG 3 cut(s) 204, 530, 1459
BstNSI RCATGY 1 cut(s) 313
BstSCI CCNGG 5 cut(s) 202, 528, 732, 1457, 1559
BstUI CGCG 2 cut(s) 918, 1206
BstV1I GCAGC 1 cut(s) 1191
BstV2I GAAGAC 1 cut(s) 111
BsuRI GGCC 5 cut(s) 245, 464, 1051, 1400, 1508
BtgI CCRYGG 1 cut(s) 341
BtgZI GCGATG 1 cut(s) 33
BtsCI GGATG 3 cut(s) 268, 370, 488
BtsI GCAGTG 1 cut(s) 1168
BtsIMutI CAGTG 4 cut(s) 170, 277, 1168, 1312
Cac8I GCNNGC 3 cut(s) 558, 633, 1010
CaiI CAGNNNCTG 2 cut(s) 134, 289
CciI TCATGA 1 cut(s) 499
CfoI GCGC 3 cut(s) 920, 1208, 1467
Cfr13I GGNCC 7 cut(s) 92, 215, 244, 541, 1050, 1144, 1563
CviAII CATG 5 cut(s) 310, 500, 751, 1078, 1141
DdeI CTNAG 7 cut(s) 453, 615, 690, 816, 987, 1175, 1363
DpnI GATC 7 cut(s) 5, 339, 651, 696, 1230, 1247, 1374
DpnII GATC 7 cut(s) 3, 337, 649, 694, 1228, 1245, 1372
EaeI YGGCCR 2 cut(s) 462, 1398
Eam1104I CTCTTC 1 cut(s) 888
EarI CTCTTC 1 cut(s) 888
Eco130I CCWWGG 1 cut(s) 1084
Eco31I GGTCTC 1 cut(s) 570
Eco47I GGWCC 5 cut(s) 92, 215, 541, 1144, 1563
Eco47III AGCGCT 1 cut(s) 1466
Eco57I CTGAAG 1 cut(s) 557
Eco88I CYCGRG 1 cut(s) 493
EcoO109I RGGNCCY 2 cut(s) 215, 244
EcoRI GAATTC 3 cut(s) 124, 139, 415
EcoRII CCWGG 3 cut(s) 202, 528, 1457
EcoT14I CCWWGG 1 cut(s) 1084
EcoT22I ATGCAT 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 1084
FaeI CATG 5 cut(s) 313, 503, 754, 1081, 1144
FalI AAGNNNNNCTT 2 cut(s) 1513, 1545
FaqI GGGAC 2 cut(s) 251, 1130
FatI CATG 5 cut(s) 309, 499, 750, 1077, 1140
FbaI TGATCA 5 cut(s) 3, 649, 1228, 1245, 1372
Fnu4HI GCNGC 1 cut(s) 1180
FokI GGATG 3 cut(s) 275, 377, 475
Fsp4HI GCNGC 1 cut(s) 1180
FspBI CTAG 1 cut(s) 177
GlaI GCGC 3 cut(s) 919, 1207, 1466
GluI GCNGC 1 cut(s) 1180
HaeII RGCGCY 1 cut(s) 1468
HaeIII GGCC 5 cut(s) 245, 464, 1051, 1400, 1508
HapII CCGG 2 cut(s) 733, 1561
HhaI GCGC 3 cut(s) 920, 1208, 1467
Hin1II CATG 5 cut(s) 313, 503, 754, 1081, 1144
Hin6I GCGC 3 cut(s) 918, 1206, 1465
HinP1I GCGC 3 cut(s) 918, 1206, 1465
HindIII AAGCTT 1 cut(s) 629
HinfI GANTC 8 cut(s) 496, 686, 814, 925, 1267, 1309, 1318, 1451
HpaII CCGG 2 cut(s) 733, 1561
HphI GGTGA 2 cut(s) 445, 1100
Hpy166II GTNNAC 1 cut(s) 182
Hpy188I TCNGA 8 cut(s) 480, 691, 819, 1018, 1126, 1308, 1332, 1366
Hpy188III TCNNGA 7 cut(s) 144, 298, 500, 515, 614, 1106, 1376
Hpy8I GTNNAC 1 cut(s) 182
HpyAV CCTTC 6 cut(s) 48, 355, 502, 883, 941, 1423
HpyCH4III ACNGT 3 cut(s) 174, 1024, 1038
HpyCH4IV ACGT 1 cut(s) 1447
HpyCH4V TGCA 7 cut(s) 62, 275, 442, 757, 836, 981, 1140
HpyF10VI GCNNNNNNNGC 6 cut(s) 242, 382, 632, 1179, 1205, 1535
HpyF3I CTNAG 7 cut(s) 453, 615, 690, 816, 987, 1175, 1363
HpySE526I ACGT 1 cut(s) 1447
Hsp92II CATG 5 cut(s) 313, 503, 754, 1081, 1144
HspAI GCGC 3 cut(s) 918, 1206, 1465
Ksp22I TGATCA 5 cut(s) 3, 649, 1228, 1245, 1372
Kzo9I GATC 7 cut(s) 3, 337, 649, 694, 1228, 1245, 1372
LguI GCTCTTC 1 cut(s) 888
LmnI GCTCC 1 cut(s) 553
Lsp1109I GCAGC 1 cut(s) 1191
LweI GCATC 3 cut(s) 49, 613, 766
MaeI CTAG 1 cut(s) 177
MaeII ACGT 1 cut(s) 1447
MaeIII GTNAC 3 cut(s) 433, 1166, 1310
MalI GATC 7 cut(s) 5, 339, 651, 696, 1230, 1247, 1374
MboI GATC 7 cut(s) 3, 337, 649, 694, 1228, 1245, 1372
MboII GAAGA 8 cut(s) 111, 358, 517, 563, 784, 905, 1109, 1424
MhlI GDGCHC 1 cut(s) 900
MlsI TGGCCA 2 cut(s) 464, 1400
MluNI TGGCCA 2 cut(s) 464, 1400
MlyI GAGTC 4 cut(s) 505, 680, 808, 1318
MmeI TCCRAC 1 cut(s) 531
Mox20I TGGCCA 2 cut(s) 464, 1400
Mph1103I ATGCAT 1 cut(s) 64
MroXI GAANNNNTTC 2 cut(s) 37, 1101
MscI TGGCCA 2 cut(s) 464, 1400
MseI TTAA 4 cut(s) 81, 105, 596, 716
Msp20I TGGCCA 2 cut(s) 464, 1400
MspA1I CMGCKG 1 cut(s) 1135
MspI CCGG 2 cut(s) 733, 1561
MspR9I CCNGG 5 cut(s) 204, 530, 734, 1459, 1561
MvaI CCWGG 3 cut(s) 204, 530, 1459
MvnI CGCG 2 cut(s) 918, 1206
MwoI GCNNNNNNNGC 6 cut(s) 242, 382, 632, 1179, 1205, 1535
NciI CCSGG 2 cut(s) 734, 1561
NdeII GATC 7 cut(s) 3, 337, 649, 694, 1228, 1245, 1372
NlaIII CATG 5 cut(s) 313, 503, 754, 1081, 1144
NlaIV GGNNCC 4 cut(s) 94, 235, 555, 1146
NmeAIII GCCGAG 1 cut(s) 276
NmuCI GTSAC 3 cut(s) 433, 1166, 1310
NsiI ATGCAT 1 cut(s) 64
NspI RCATGY 1 cut(s) 313
NspV TTCGAA 2 cut(s) 41, 122
PagI TCATGA 1 cut(s) 499
PciI ACATGT 1 cut(s) 309
PciSI GCTCTTC 1 cut(s) 888
PdmI GAANNNNTTC 2 cut(s) 37, 1101
PfeI GAWTC 4 cut(s) 925, 1267, 1318, 1451
PflFI GACNNNGTC 1 cut(s) 1514
PflMI CCANNNNNTGG 1 cut(s) 550
PkrI GCNGC 1 cut(s) 1181
PleI GAGTC 4 cut(s) 504, 680, 808, 1317
PpsI GAGTC 4 cut(s) 504, 680, 808, 1317
PpuMI RGGWCCY 1 cut(s) 215
PscI ACATGT 1 cut(s) 309
Psp5II RGGWCCY 1 cut(s) 215
Psp6I CCWGG 3 cut(s) 202, 528, 1457
PspGI CCWGG 3 cut(s) 202, 528, 1457
PspN4I GGNNCC 4 cut(s) 94, 235, 555, 1146
PspPI GGNCC 7 cut(s) 92, 215, 244, 541, 1050, 1144, 1563
PspPPI RGGWCCY 1 cut(s) 215
PstNI CAGNNNCTG 2 cut(s) 134, 289
PsyI GACNNNGTC 1 cut(s) 1514
SapI GCTCTTC 1 cut(s) 888
SaqAI TTAA 4 cut(s) 81, 105, 596, 716
SatI GCNGC 1 cut(s) 1180
Sau3AI GATC 7 cut(s) 3, 337, 649, 694, 1228, 1245, 1372
Sau96I GGNCC 7 cut(s) 92, 215, 244, 541, 1050, 1144, 1563
SchI GAGTC 4 cut(s) 505, 680, 808, 1318
ScrFI CCNGG 5 cut(s) 204, 530, 734, 1459, 1561
SduI GDGCHC 1 cut(s) 900
SfaNI GCATC 3 cut(s) 49, 613, 766
SfuI TTCGAA 2 cut(s) 41, 122
SinI GGWCC 5 cut(s) 92, 215, 541, 1144, 1563
SmlI CTYRAG 3 cut(s) 386, 515, 1519
SmoI CTYRAG 3 cut(s) 386, 515, 1519
SsiI CCGC 3 cut(s) 916, 1135, 1258
SspMI CTAG 1 cut(s) 177
StyD4I CCNGG 5 cut(s) 202, 528, 732, 1457, 1559
StyI CCWWGG 1 cut(s) 1084
TaaI ACNGT 3 cut(s) 174, 1024, 1038
TaiI ACGT 1 cut(s) 1450
TaqI TCGA 4 cut(s) 31, 41, 122, 760
TfiI GAWTC 4 cut(s) 925, 1267, 1318, 1451
Tru1I TTAA 4 cut(s) 81, 105, 596, 716
Tru9I TTAA 4 cut(s) 81, 105, 596, 716
TscAI CASTG 4 cut(s) 177, 277, 1175, 1319
TseFI GTSAC 3 cut(s) 433, 1166, 1310
TseI GCWGC 1 cut(s) 1179
Tsp45I GTSAC 3 cut(s) 433, 1166, 1310
TspDTI ATGAA 4 cut(s) 739, 757, 793, 1431
TspGWI ACGGA 2 cut(s) 330, 1145
TspRI CASTG 4 cut(s) 177, 277, 1175, 1319
Tth111I GACNNNGTC 1 cut(s) 1514
Van91I CCANNNNNTGG 1 cut(s) 550
VpaK11BI GGWCC 5 cut(s) 92, 215, 541, 1144, 1563
XapI RAATTY 6 cut(s) 12, 124, 139, 415, 849, 1127
XceI RCATGY 1 cut(s) 313
XmnI GAANNNNTTC 2 cut(s) 37, 1101
XspI CTAG 1 cut(s) 177
Zsp2I ATGCAT 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.