pycom09g03680

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0006454
Physical Location & Seq
Reverse (-)
2717454 .. 2719563
2110 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g03680.1

Sequence Viewer

Length: 1281 bp
ATGATAAAAAATATATGGGGTAAAATACCTCGGAAACCCTCGAAATCATCGCAAAGCGATTCGTATCGTGACGGAGGGGCAAATGGGAATGGCCCAAATTCATCCCACAGCTCAAAATCGAGCTCATCTTCGTCGAATTTGGGTTCGGGCAATTCTCGTTCGAGCAATGGGGTTCTTACCGCGCACTCTTCAGGCGGTGTTAAATCGAATCACTCAAAGAAATCAGCTCCCCTTGCAACTCAAGCAGGTCCTTTGTTGTCAGCGGGTGCATACGAGGCTCTGCCGAGCTTCAAGGATGTTCCAAGTTCAGAAAGGCAGAATTATTTTATTAAGAAGTTGAATATGTGTTGTGTGGTTTTTGAATTTAATGATCCCTCGAAGAATCTCAAGGAGAAGGACGTCAAGAGGCAAACTTTGCTTGAACTTGTAGATTACATTTCATCAGTAACATCGAAGTTCAATGAGGTTGCAATGCAAGAGATTACAAAGATGGTGGCGACCAATCTGTTTCGTACATTTTCGTCTTCCAATCTTGATAACAAGGGTCCTGAAATTTATGACCAAGACGAGGAGGACCAAATGTTGGAACCAGCTTGGCCTCATCTGCAGATTGTGTATGAATGTCTATTAAGATTCGTGGCTTCTCCAGAGACCGATGCCAAGCTAGCTAAAAGATATATTGATCATTCGTTTGTGTTGAAGTTGCTTGATTTGTTTAACTCTGAGGACCAAAGAGAGAGGGAATACTTAAAGATGATTCTCCATCGCATTTATGGGAAGTTCATGGTGCATCGACCGTTCATTAGGAAATCCATCAACAACATCTTCTTTTGGTTTATTTTTGAGACTGAGAAGCACAATGGGATTGCGGAATTGCTTGAAATCTTAGGCAGCATAATCAATGGTTTTGCTTTGCCTTTGAAGGAAGAGCACAAGCTGTTCCTTGTTCGTGCCTTGATTCCTCTTCACAAGCCCAAGTGTGTATCTATGTACCATCAGCAACTCTCATATTGCATTAGTCAGTTTGTGGAGAAAGATGCCAAGCTGGCAGATACAGTGATTCGAGGACTGCTGAAGTATTGGCCCATAACGAATAGTTCAAAGGAAGTTATGTTCCTTGGTGAATTGGAAGAGGTTCTGGAAGCTACACAGCCATCAGAATTTCAGCGCTGCATGGTCCCCTTATTCCGTCAAATTGGTAACTGCCTAAACAGCTCACATTTTCAGGTAGCTGAACGTGCATTGTTTTTGTGGAATAACGATCACATAATCGAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000096 GO:0000902 GO:0000904 GO:0002682 GO:0002683 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006082 GO:0006139 GO:0006520 GO:0006534 GO:0006555 GO:0006575 GO:0006725 GO:0006732 GO:0006790 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0009058 GO:0009066 GO:0009069 GO:0009116 GO:0009119 GO:0009653 GO:0009759 GO:0009888 GO:0009966 GO:0009968 GO:0009987 GO:0010026 GO:0010090 GO:0010646 GO:0010648 GO:0016043 GO:0016143 GO:0016144 GO:0017144 GO:0018130 GO:0019222 GO:0019438 GO:0019748 GO:0019752 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0023051 GO:0023057 GO:0030154 GO:0031323 GO:0031347 GO:0031348 GO:0032502 GO:0032989 GO:0033353 GO:0034641 GO:0042278 GO:0042343 GO:0042430 GO:0042435 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0045088 GO:0045824 GO:0046128 GO:0046439 GO:0046483 GO:0046498 GO:0046500 GO:0048468 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048856 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051186 GO:0055086 GO:0065007 GO:0071704 GO:0071840 GO:0072521 GO:0080134 GO:0080183 GO:0090342 GO:0090558 GO:0090626 GO:0097159 GO:1900457 GO:1900458 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901657 GO:1901659 GO:2000377
KEGG Pathways
Metabolic & Signaling
ko03015 ko04071 ko04113 ko04114 ko04151 ko04152 ko04261 ko04728 ko05165 map03015 map04071 map04113 map04114 map04151 map04152 map04261 map04728 map05165
Pfam Domains
Protein Families
B56

Protein Analysis

427

Amino Acids

48.43

Weight (kDa)

8.31

Isoelectric Point (pI)

43.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B56 PF01603 94 - 425 1.8e-157 Protein phosphatase 2A regulatory B subunit (B56 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 402
Acc36I ACCTGC 1 cut(s) 236
AccB7I CCANNNNNTGG 1 cut(s) 583
AccII CGCG 1 cut(s) 182
AciI CCGC 4 cut(s) 180, 195, 263, 869
AclWI GGATC 1 cut(s) 365
AcsI RAATTY 6 cut(s) 97, 136, 362, 552, 1160, 1275
AcuI CTGAAG 2 cut(s) 174, 1094
AcyI GRCGYC 1 cut(s) 399
AfaI GTAC 2 cut(s) 514, 992
AfeI AGCGCT 1 cut(s) 1169
AfiI CCNNNNNNNGG 2 cut(s) 568, 583
AgsI TTSAA 9 cut(s) 292, 340, 362, 422, 460, 700, 881, 922, 1101
AloI GAACNNNNNNTCC 2 cut(s) 1097, 1129
Alw21I GWGCWC 2 cut(s) 125, 933
Alw26I GTCTC 2 cut(s) 644, 839
AlwI GGATC 1 cut(s) 365
Aor51HI AGCGCT 1 cut(s) 1169
AoxI GGCC 3 cut(s) 91, 596, 1082
ApeKI GCWGC 2 cut(s) 891, 1170
ApoI RAATTY 6 cut(s) 97, 136, 362, 552, 1160, 1275
Asp700I GAANNNNTTC 1 cut(s) 1134
AspLEI GCGC 2 cut(s) 184, 1170
AspS9I GGNCC 7 cut(s) 92, 248, 545, 574, 727, 1083, 1177
AsuHPI GGTGA 1 cut(s) 1133
AsuNHI GCTAGC 1 cut(s) 664
AvaII GGWCC 5 cut(s) 248, 545, 574, 727, 1177
BanII GRGCYC 1 cut(s) 125
BbsI GAAGAC 1 cut(s) 516
Bbv12I GWGCWC 2 cut(s) 125, 933
BbvI GCAGC 2 cut(s) 903, 1157
BccI CCATC 5 cut(s) 484, 771, 821, 1002, 1162
BclI TGATCA 1 cut(s) 682
BcoDI GTCTC 2 cut(s) 644, 839
BfaI CTAG 1 cut(s) 665
BfmI CTRYAG 1 cut(s) 605
BfoI RGCGCY 1 cut(s) 1171
BfuAI ACCTGC 1 cut(s) 236
BglI GCCNNNNNGGC 1 cut(s) 1046
BisI GCNGC 2 cut(s) 892, 1171
BlsI GCNGC 2 cut(s) 893, 1172
Bme18I GGWCC 5 cut(s) 248, 545, 574, 727, 1177
BmgT120I GGNCC 7 cut(s) 92, 248, 545, 574, 727, 1083, 1177
BmiI GGNNCC 3 cut(s) 546, 588, 1179
BmsI GCATC 3 cut(s) 646, 799, 1027
BmtI GCTAGC 1 cut(s) 668
BpiI GAAGAC 1 cut(s) 516
BpmI CTGGAG 1 cut(s) 630
BpuEI CTTGAG 2 cut(s) 225, 371
BsaBI GATNNNNATC 1 cut(s) 63
BsaHI GRCGYC 1 cut(s) 399
BsaI GGTCTC 1 cut(s) 644
BsaJI CCNNGG 2 cut(s) 29, 1117
Bsc4I CCNNNNNNNGG 2 cut(s) 568, 583
Bse3DI GCAATG 2 cut(s) 172, 477
Bse8I GATNNNNATC 1 cut(s) 63
BseDI CCNNGG 2 cut(s) 29, 1117
BseGI GGATG 2 cut(s) 101, 301
BseJI GATNNNNATC 1 cut(s) 63
BseLI CCNNNNNNNGG 2 cut(s) 568, 583
BseMI GCAATG 2 cut(s) 172, 477
BseMII CTCAG 2 cut(s) 714, 840
BseRI GAGGAG 1 cut(s) 584
BseXI GCAGC 2 cut(s) 903, 1157
Bsh1236I CGCG 1 cut(s) 182
Bsh1285I CGRYCG 1 cut(s) 797
BshFI GGCC 3 cut(s) 93, 598, 1084
BsiEI CGRYCG 1 cut(s) 797
BsiHKAI GWGCWC 2 cut(s) 125, 933
BslFI GGGAC 1 cut(s) 1163
BslI CCNNNNNNNGG 2 cut(s) 568, 583
BsmAI GTCTC 2 cut(s) 644, 839
BsmFI GGGAC 1 cut(s) 1163
BsnI GGCC 3 cut(s) 93, 598, 1084
Bso31I GGTCTC 1 cut(s) 644
Bsp1286I GDGCHC 2 cut(s) 125, 933
Bsp143I GATC 3 cut(s) 370, 682, 1261
BspACI CCGC 4 cut(s) 180, 195, 263, 869
BspANI GGCC 3 cut(s) 93, 598, 1084
BspCNI CTCAG 2 cut(s) 715, 841
BspFNI CGCG 1 cut(s) 182
BspLI GGNNCC 3 cut(s) 546, 588, 1179
BspMAI CTGCAG 1 cut(s) 609
BspMI ACCTGC 1 cut(s) 236
BspOI GCTAGC 1 cut(s) 668
BspPI GGATC 1 cut(s) 365
BspQI GCTCTTC 1 cut(s) 921
BspTNI GGTCTC 1 cut(s) 644
BsrDI GCAATG 2 cut(s) 172, 477
BssECI CCNNGG 2 cut(s) 29, 1117
BssMI GATC 3 cut(s) 370, 682, 1261
BssNI GRCGYC 1 cut(s) 399
BssT1I CCWWGG 1 cut(s) 1117
Bst4CI ACNGT 2 cut(s) 798, 1057
Bst6I CTCTTC 4 cut(s) 193, 921, 969, 1125
BstACI GRCGYC 1 cut(s) 399
BstAPI GCANNNNNTGC 1 cut(s) 415
BstC8I GCNNGC 2 cut(s) 666, 1047
BstDEI CTNAG 3 cut(s) 723, 849, 886
BstF5I GGATG 2 cut(s) 101, 301
BstFNI CGCG 1 cut(s) 182
BstH2I RGCGCY 1 cut(s) 1171
BstHHI GCGC 2 cut(s) 184, 1170
BstKTI GATC 3 cut(s) 373, 685, 1264
BstMAI GTCTC 2 cut(s) 644, 839
BstMBI GATC 3 cut(s) 370, 682, 1261
BstMCI CGRYCG 1 cut(s) 797
BstMWI GCNNNNNNNGC 9 cut(s) 233, 242, 275, 415, 604, 665, 1046, 1212, 1238
BstSFI CTRYAG 1 cut(s) 605
BstUI CGCG 1 cut(s) 182
BstV1I GCAGC 2 cut(s) 903, 1157
BstV2I GAAGAC 1 cut(s) 516
BsuRI GGCC 3 cut(s) 93, 598, 1084
BtgZI GCGATG 2 cut(s) 33, 749
BtsCI GGATG 2 cut(s) 101, 301
BtsIMutI CAGTG 1 cut(s) 1062
BveI ACCTGC 1 cut(s) 236
Cac8I GCNNGC 2 cut(s) 666, 1047
CfoI GCGC 2 cut(s) 184, 1170
Cfr13I GGNCC 7 cut(s) 92, 248, 545, 574, 727, 1083, 1177
Csp6I GTAC 2 cut(s) 513, 991
CspCI CAANNNNNGTGG 2 cut(s) 474, 509
CviAII CATG 2 cut(s) 784, 1174
CviQI GTAC 2 cut(s) 513, 991
DdeI CTNAG 3 cut(s) 723, 849, 886
DpnI GATC 3 cut(s) 372, 684, 1263
DpnII GATC 3 cut(s) 370, 682, 1261
Eam1104I CTCTTC 4 cut(s) 193, 921, 969, 1125
EarI CTCTTC 4 cut(s) 193, 921, 969, 1125
Ecl136II GAGCTC 1 cut(s) 123
Eco130I CCWWGG 1 cut(s) 1117
Eco24I GRGCYC 1 cut(s) 125
Eco31I GGTCTC 1 cut(s) 644
Eco47I GGWCC 5 cut(s) 248, 545, 574, 727, 1177
Eco47III AGCGCT 1 cut(s) 1169
Eco53kI GAGCTC 1 cut(s) 123
Eco57I CTGAAG 2 cut(s) 174, 1094
EcoICRI GAGCTC 1 cut(s) 123
EcoO109I RGGNCCY 2 cut(s) 248, 545
EcoT14I CCWWGG 1 cut(s) 1117
EcoT38I GRGCYC 1 cut(s) 125
ErhI CCWWGG 1 cut(s) 1117
FaeI CATG 2 cut(s) 787, 1177
FaqI GGGAC 1 cut(s) 1163
FatI CATG 2 cut(s) 783, 1173
FauI CCCGC 1 cut(s) 256
FbaI TGATCA 1 cut(s) 682
Fnu4HI GCNGC 2 cut(s) 892, 1171
FokI GGATG 2 cut(s) 88, 308
FriOI GRGCYC 1 cut(s) 125
Fsp4HI GCNGC 2 cut(s) 892, 1171
FspBI CTAG 1 cut(s) 665
GlaI GCGC 2 cut(s) 183, 1169
GluI GCNGC 2 cut(s) 892, 1171
GsuI CTGGAG 1 cut(s) 630
HaeII RGCGCY 1 cut(s) 1171
HaeIII GGCC 3 cut(s) 93, 598, 1084
HhaI GCGC 2 cut(s) 184, 1170
Hin1I GRCGYC 1 cut(s) 399
Hin1II CATG 2 cut(s) 787, 1177
Hin6I GCGC 2 cut(s) 182, 1168
HinP1I GCGC 2 cut(s) 182, 1168
HinfI GANTC 7 cut(s) 59, 208, 382, 633, 757, 958, 1060
HphI GGTGA 1 cut(s) 1133
Hpy188I TCNGA 4 cut(s) 33, 310, 724, 1159
Hpy188III TCNNGA 6 cut(s) 68, 403, 533, 548, 647, 1139
Hpy99I CGWCG 1 cut(s) 136
HpyAV CCTTC 2 cut(s) 388, 916
HpyCH4III ACNGT 2 cut(s) 798, 1057
HpyCH4IV ACGT 2 cut(s) 399, 1237
HpyCH4V TGCA 9 cut(s) 236, 269, 470, 475, 607, 790, 1014, 1173, 1241
HpyF10VI GCNNNNNNNGC 9 cut(s) 233, 242, 275, 415, 604, 665, 1046, 1212, 1238
HpyF3I CTNAG 3 cut(s) 723, 849, 886
HpySE526I ACGT 2 cut(s) 399, 1237
Hsp92I GRCGYC 1 cut(s) 399
Hsp92II CATG 2 cut(s) 787, 1177
HspAI GCGC 2 cut(s) 182, 1168
Ksp22I TGATCA 1 cut(s) 682
Kzo9I GATC 3 cut(s) 370, 682, 1261
LguI GCTCTTC 1 cut(s) 921
LmnI GCTCC 1 cut(s) 232
LpnPI CCDG 8 cut(s) 177, 231, 561, 603, 660, 1031, 1124, 1211
Lsp1109I GCAGC 2 cut(s) 903, 1157
LweI GCATC 3 cut(s) 646, 799, 1027
MaeI CTAG 1 cut(s) 665
MaeII ACGT 2 cut(s) 399, 1237
MaeIII GTNAC 3 cut(s) 68, 445, 1199
MalI GATC 3 cut(s) 372, 684, 1263
MboI GATC 3 cut(s) 370, 682, 1261
MboII GAAGA 8 cut(s) 120, 180, 391, 516, 817, 938, 956, 1142
MhlI GDGCHC 2 cut(s) 125, 933
MmeI TCCRAC 1 cut(s) 564
MroXI GAANNNNTTC 1 cut(s) 1134
MseI TTAA 6 cut(s) 201, 330, 366, 629, 717, 749
MspA1I CMGCKG 1 cut(s) 263
MvnI CGCG 1 cut(s) 182
MwoI GCNNNNNNNGC 9 cut(s) 233, 242, 275, 415, 604, 665, 1046, 1212, 1238
NdeII GATC 3 cut(s) 370, 682, 1261
NheI GCTAGC 1 cut(s) 664
NlaIII CATG 2 cut(s) 787, 1177
NlaIV GGNNCC 3 cut(s) 546, 588, 1179
NmeAIII GCCGAG 1 cut(s) 309
NmuCI GTSAC 1 cut(s) 68
PciSI GCTCTTC 1 cut(s) 921
PdmI GAANNNNTTC 1 cut(s) 1134
PfeI GAWTC 7 cut(s) 59, 208, 382, 633, 757, 958, 1060
PflMI CCANNNNNTGG 1 cut(s) 583
PkrI GCNGC 2 cut(s) 893, 1172
PpuMI RGGWCCY 2 cut(s) 248, 545
Psp124BI GAGCTC 1 cut(s) 125
Psp5II RGGWCCY 2 cut(s) 248, 545
PspN4I GGNNCC 3 cut(s) 546, 588, 1179
PspPI GGNCC 7 cut(s) 92, 248, 545, 574, 727, 1083, 1177
PspPPI RGGWCCY 2 cut(s) 248, 545
PstI CTGCAG 1 cut(s) 609
RsaI GTAC 2 cut(s) 514, 992
RsaNI GTAC 2 cut(s) 513, 991
SacI GAGCTC 1 cut(s) 125
SapI GCTCTTC 1 cut(s) 921
SaqAI TTAA 6 cut(s) 201, 330, 366, 629, 717, 749
SatI GCNGC 2 cut(s) 892, 1171
Sau3AI GATC 3 cut(s) 370, 682, 1261
Sau96I GGNCC 7 cut(s) 92, 248, 545, 574, 727, 1083, 1177
SduI GDGCHC 2 cut(s) 125, 933
SfaNI GCATC 3 cut(s) 646, 799, 1027
SfcI CTRYAG 1 cut(s) 605
SinI GGWCC 5 cut(s) 248, 545, 574, 727, 1177
SmlI CTYRAG 2 cut(s) 240, 386
SmoI CTYRAG 2 cut(s) 240, 386
SsiI CCGC 4 cut(s) 180, 195, 263, 869
SspMI CTAG 1 cut(s) 665
SstI GAGCTC 1 cut(s) 125
StyI CCWWGG 1 cut(s) 1117
TaaI ACNGT 2 cut(s) 798, 1057
TaiI ACGT 2 cut(s) 402, 1240
TaqII GACCGA 1 cut(s) 668
TfiI GAWTC 7 cut(s) 59, 208, 382, 633, 757, 958, 1060
Tru1I TTAA 6 cut(s) 201, 330, 366, 629, 717, 749
Tru9I TTAA 6 cut(s) 201, 330, 366, 629, 717, 749
TscAI CASTG 1 cut(s) 1062
TseFI GTSAC 1 cut(s) 68
TseI GCWGC 2 cut(s) 891, 1170
Tsp45I GTSAC 1 cut(s) 68
TspDTI ATGAA 5 cut(s) 90, 429, 633, 772, 790
TspGWI ACGGA 2 cut(s) 87, 1178
TspRI CASTG 1 cut(s) 1062
Van91I CCANNNNNTGG 1 cut(s) 583
VpaK11BI GGWCC 5 cut(s) 248, 545, 574, 727, 1177
XapI RAATTY 6 cut(s) 97, 136, 362, 552, 1160, 1275
XcmI CCANNNNNNNNNTGG 1 cut(s) 770
XmnI GAANNNNTTC 1 cut(s) 1134
XspI CTAG 1 cut(s) 665
ZraI GACGTC 1 cut(s) 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.