AT3G24530

AAA-type ATPase family protein ankyrin repeat family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0011301
Physical Location & Seq
Reverse (-)
8945385 .. 8948017
2633 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G24530.2

Sequence Viewer

Length: 1446 bp
ATGAACCGACAAAACGGTGGAGGGCAGCGGCTGAGATCCGCTAGACCCACCACAATTCACGATTGTGCTCTCTCCGGCGATCTTATTGCTTTACAGAGACTTCTTAAAGACAATCCTTCTCTTCTCAATGAACGCAATCCCGTCATGTATCATACGCCACTTCATGTTTCTGCTGGTAATGGTAATGTTGATATTGTTAAGTATCTTCTCGCTTGGACTGGATCTGATAAAGTTGAGTTAGAAGCAATGAATACTTATGGTGAGACTCCATTGCATATGGCAGCTAAGAACGGTTGTAATGAAGCTGCAAAGCTACTCCTTGAGAGTGGTGCTTTTATCGAAGCTAAAGCCAGCAATGGTATGACACCGTTGCATCTTGCGGTATGGTACTCAATTACTGCTAAAGAGATCTCGACTGTTAAGACACTGCTTGATCATAACGCAGATTGCAGTGCAAAAGATAATGAAGGGATGACTCCTTTGGACCATCTGCCTCAAGGGCAAGGCAGTGAGAAGTTACGGGAATTACTGCGGTGGTTTCTCCAAGAGCAGAGGAAAAGAAGTGCGCTTGAGCAATGTGGTAAGACAAAAGCCAAGATGGAACTACTTGAGGATGAGTTATCAAATATTGTTGGATTAAGTGAGCTGAAGACACAACTGAGGAAATGGGCGAAAGGAATGCTTTTAGATGAAAGGCGAAGGGCTCTTGGGCTAAATATTGGAACCCGAAGACCTCCGCATATGGCATTTCTTGGAAACCCTGGAACAGGTAAAACCATGGTAGCTCGAGTTCTCGGGAAGCTGCTTAACACGGTTGGAATTTTACCAACTGATAAGGTAACAGAAGTACAGCGTACAGATTTGGTGGGTGAGTTTGTTGGCCATACAGGACCAAAGACCAGGAGAAAGATCCAAGAAGCTGAGGGAGGAATTCTGTTTGTGGATGAAGCATACAGATTGATCCCGATGCAAAAGGCGGACGACAAGGACTACGGTCTAGAAGCTCTGGAAGAAATCATGTCAGTCATGGACACAGGGAAAATTGTGGTGATATTTGCAGGATACAGCGAGCCCATGAAGCGTGTGATCGCATCAAACGAAGGGTTTTGCAGAAGGGTCACAAAGTTTTTCAATTTCAGCGACTTCAGTGCCAAGGAACTGGCGCAGATACTACACATCAAGATGAACAACCAAGGAGAGGACACTCTGTTCTATGGTTTCAGATTGCATGAGTCCTGCACTTTGCAGGAGATAGCGTCACTGATAGAGGCAGAAACAACGGAAAAGCAGAGGAAAGAGATGAATGGAGGATTGGTGGACACGTTACTTGTTAACGCGAGGGAGAATCTTGATCTCCGACTTAGCTTTGAGTGTGTTGACACTGAAGAGATTTGTACAATCAAGTTGGAGGATCTAGAGGCCGGGCTTCGAGTTTTCTCGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA Ank Ank_2 Ank_3

Protein Analysis

481

Amino Acids

53.67

Weight (kDa)

6.19

Isoelectric Point (pI)

37.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 3 - 68 9.8e-14 Ankyrin repeats (3 copies)
Ank_2 PF12796 35 - 106 1.4e-13 Ankyrin repeats (3 copies)
Ank_5 PF13857 36 - 79 1.4e-06 Ankyrin repeats (many copies)
Ank PF00023 38 - 67 3.2e-06 Ankyrin repeat
Ank_4 PF13637 43 - 81 1.8e-07 Ankyrin repeats (many copies)
AAA PF00004 201 - 316 9.3e-13 ATPase family associated with various cellular activities (AAA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24530 AT3G24530 AT3G24530
fragaria_vesca FvH4_1g04710
malus_domestica MD02G1046000.v1.1 MD15G1184200.v1.1
prunus_persica Prupe.7G232000_v2.0.a1 Prupe.7G232000_v2.0.a1
pyrus_communis pycom02g03840 pycom02g03850 pycom15g16580
rosa_chinensis RchiOBHm_Chr2g0090311
rosa_laevigata RLG00000016097
rosa_multiflora Rmu_co8096142.1_g000001 Rmu_sc0006235.1_g000004
rosa_roxburghii Rroxscaffold_2G00151290
rosa_rugosa Rorug02G0006500
rosa_samantha Rh2CG053200 Rh2DG052300
rosa_wichuraiana Rw2G004450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1337
AciI CCGC 6 cut(s) 28, 39, 380, 532, 737, 977
AclWI GGATC 5 cut(s) 30, 229, 904, 955, 1419
AcoI YGGCCR 1 cut(s) 880
AcsI RAATTY 2 cut(s) 819, 930
AcuI CTGAAG 3 cut(s) 668, 1129, 1404
AfaI GTAC 4 cut(s) 389, 849, 856, 1396
AfiI CCNNNNNNNGG 2 cut(s) 767, 1198
AflIII ACRYGT 1 cut(s) 1320
AgsI TTSAA 1 cut(s) 1132
AjnI CCWGG 2 cut(s) 760, 899
AjuI GAANNNNNNNTTGG 2 cut(s) 1295, 1327
AleI CACNNNNGTG 1 cut(s) 63
AloI GAACNNNNNNTCC 2 cut(s) 1193, 1225
Alw21I GWGCWC 1 cut(s) 70
Alw26I GTCTC 2 cut(s) 91, 257
AlwI GGATC 5 cut(s) 30, 229, 904, 955, 1419
AlwNI CAGNNNCTG 1 cut(s) 31
Ama87I CYCGRG 2 cut(s) 786, 794
AoxI GGCC 2 cut(s) 880, 1419
ApeKI GCWGC 4 cut(s) 25, 281, 305, 802
ApoI RAATTY 2 cut(s) 819, 930
AspLEI GCGC 2 cut(s) 568, 1165
AspS9I GGNCC 2 cut(s) 484, 890
AsuC2I CCSGG 1 cut(s) 1423
AsuHPI GGTGA 3 cut(s) 272, 881, 1060
AvaI CYCGRG 2 cut(s) 786, 794
AvaII GGWCC 2 cut(s) 484, 890
BalI TGGCCA 1 cut(s) 882
BanII GRGCYC 2 cut(s) 706, 1074
BbsI GAAGAC 2 cut(s) 656, 736
Bbv12I GWGCWC 1 cut(s) 70
BbvCI CCTCAGC 1 cut(s) 921
BbvI GCAGC 4 cut(s) 37, 292, 293, 789
BccI CCATC 2 cut(s) 495, 592
BcgI CGANNNNNNTGC 6 cut(s) 68, 102, 661, 695, 1130, 1164
BciT130I CCWGG 2 cut(s) 762, 901
BciVI GTATCC 1 cut(s) 1055
BclI TGATCA 1 cut(s) 433
BcnI CCSGG 1 cut(s) 1423
BcoDI GTCTC 2 cut(s) 91, 257
BfaI CTAG 3 cut(s) 42, 998, 1415
BfuI GTATCC 1 cut(s) 1055
BglI GCCNNNNNGGC 1 cut(s) 499
BglII AGATCT 1 cut(s) 408
BisI GCNGC 5 cut(s) 26, 29, 282, 306, 803
BlsI GCNGC 5 cut(s) 27, 30, 283, 307, 804
Bme1390I CCNGG 3 cut(s) 762, 901, 1423
Bme18I GGWCC 2 cut(s) 484, 890
BmeT110I CYCGRG 2 cut(s) 786, 794
BmgT120I GGNCC 2 cut(s) 484, 890
BmiI GGNNCC 1 cut(s) 724
BmrFI CCNGG 3 cut(s) 762, 901, 1423
BmsI GCATC 3 cut(s) 382, 957, 1100
BoxI GACNNNNGTC 1 cut(s) 993
BpiI GAAGAC 2 cut(s) 656, 736
Bpu10I CCTNAGC 1 cut(s) 921
BpuEI CTTGAG 4 cut(s) 341, 480, 590, 629
BpuMI CCSGG 1 cut(s) 1423
BsaJI CCNNGG 4 cut(s) 760, 777, 1152, 1192
BsaXI ACNNNNNCTCC 4 cut(s) 1241, 1271, 1299, 1329
Bsc4I CCNNNNNNNGG 2 cut(s) 767, 1198
Bse1I ACTGG 2 cut(s) 223, 1164
Bse3DI GCAATG 4 cut(s) 252, 269, 361, 581
BseBI CCWGG 2 cut(s) 762, 901
BseDI CCNNGG 4 cut(s) 760, 777, 1152, 1192
BseGI GGATG 3 cut(s) 477, 619, 949
BseLI CCNNNNNNNGG 2 cut(s) 767, 1198
BseMI GCAATG 4 cut(s) 252, 269, 361, 581
BseMII CTCAG 3 cut(s) 23, 650, 912
BseNI ACTGG 2 cut(s) 223, 1164
BseXI GCAGC 4 cut(s) 37, 292, 293, 789
BsgI GTGCAG 1 cut(s) 1222
Bsh1236I CGCG 1 cut(s) 1337
BshFI GGCC 2 cut(s) 882, 1421
BsiHKAI GWGCWC 1 cut(s) 70
BsiHKCI CYCGRG 2 cut(s) 786, 794
BsiSI CCGG 2 cut(s) 75, 1422
BslI CCNNNNNNNGG 2 cut(s) 767, 1198
BsmAI GTCTC 2 cut(s) 91, 257
BsmI GAATGC 1 cut(s) 684
BsnI GGCC 2 cut(s) 882, 1421
BsoBI CYCGRG 2 cut(s) 786, 794
Bsp1286I GDGCHC 3 cut(s) 70, 706, 1074
Bsp1407I TGTACA 1 cut(s) 1394
Bsp19I CCATGG 1 cut(s) 777
BspACI CCGC 6 cut(s) 28, 39, 380, 532, 737, 977
BspANI GGCC 2 cut(s) 882, 1421
BspCNI CTCAG 3 cut(s) 24, 651, 913
BspFNI CGCG 1 cut(s) 1337
BspLI GGNNCC 1 cut(s) 724
BspPI GGATC 5 cut(s) 30, 229, 904, 955, 1419
BsrDI GCAATG 4 cut(s) 252, 269, 361, 581
BsrGI TGTACA 1 cut(s) 1394
BsrI ACTGG 2 cut(s) 223, 1164
BssECI CCNNGG 4 cut(s) 760, 777, 1152, 1192
BssT1I CCWWGG 3 cut(s) 777, 1152, 1192
Bst2UI CCWGG 2 cut(s) 762, 901
Bst4CI ACNGT 6 cut(s) 17, 293, 369, 418, 814, 995
Bst6I CTCTTC 2 cut(s) 126, 1380
BstAUI TGTACA 1 cut(s) 1394
BstC8I GCNNGC 2 cut(s) 352, 1070
BstDEI CTNAG 5 cut(s) 32, 285, 659, 921, 1361
BstDSI CCRYGG 1 cut(s) 777
BstENI CCTNNNNNAGG 1 cut(s) 765
BstF5I GGATG 3 cut(s) 477, 619, 949
BstFNI CGCG 1 cut(s) 1337
BstHHI GCGC 2 cut(s) 568, 1165
BstMAI GTCTC 2 cut(s) 91, 257
BstMWI GCNNNNNNNGC 2 cut(s) 499, 1078
BstNI CCWGG 2 cut(s) 762, 901
BstPAI GACNNNNGTC 1 cut(s) 993
BstSCI CCNGG 3 cut(s) 760, 899, 1421
BstUI CGCG 1 cut(s) 1337
BstV1I GCAGC 4 cut(s) 37, 292, 293, 789
BstV2I GAAGAC 2 cut(s) 656, 736
BstX2I RGATCY 5 cut(s) 35, 221, 408, 909, 1411
BstXI CCANNNNNNTGG 1 cut(s) 1159
BstYI RGATCY 5 cut(s) 35, 221, 408, 909, 1411
BsuI GTATCC 1 cut(s) 1055
BsuRI GGCC 2 cut(s) 882, 1421
BtgI CCRYGG 1 cut(s) 777
BtsCI GGATG 3 cut(s) 477, 619, 949
BtsI GCAGTG 3 cut(s) 425, 457, 514
BtsIMutI CAGTG 6 cut(s) 425, 457, 514, 1153, 1259, 1380
Cac8I GCNNGC 2 cut(s) 352, 1070
CaiI CAGNNNCTG 1 cut(s) 31
CfoI GCGC 2 cut(s) 568, 1165
Cfr13I GGNCC 2 cut(s) 484, 890
CseI GACGC 1 cut(s) 1245
Csp6I GTAC 4 cut(s) 388, 848, 855, 1395
CviAII CATG 7 cut(s) 145, 164, 778, 1018, 1027, 1075, 1229
CviQI GTAC 4 cut(s) 388, 848, 855, 1395
DdeI CTNAG 5 cut(s) 32, 285, 659, 921, 1361
EaeI YGGCCR 1 cut(s) 880
Eam1104I CTCTTC 2 cut(s) 126, 1380
EarI CTCTTC 2 cut(s) 126, 1380
EciI GGCGGA 1 cut(s) 992
Eco130I CCWWGG 3 cut(s) 777, 1152, 1192
Eco24I GRGCYC 2 cut(s) 706, 1074
Eco47I GGWCC 2 cut(s) 484, 890
Eco57I CTGAAG 3 cut(s) 668, 1129, 1404
Eco88I CYCGRG 2 cut(s) 786, 794
EcoNI CCTNNNNNAGG 1 cut(s) 765
EcoRI GAATTC 1 cut(s) 930
EcoRII CCWGG 2 cut(s) 760, 899
EcoT14I CCWWGG 3 cut(s) 777, 1152, 1192
EcoT38I GRGCYC 2 cut(s) 706, 1074
ErhI CCWWGG 3 cut(s) 777, 1152, 1192
FaeI CATG 7 cut(s) 148, 167, 781, 1021, 1030, 1078, 1232
FalI AAGNNNNNCTT 2 cut(s) 666, 698
FatI CATG 7 cut(s) 144, 163, 777, 1017, 1026, 1074, 1228
FauNDI CATATG 2 cut(s) 276, 741
FbaI TGATCA 1 cut(s) 433
Fnu4HI GCNGC 5 cut(s) 26, 29, 282, 306, 803
FokI GGATG 3 cut(s) 484, 626, 956
FriOI GRGCYC 2 cut(s) 706, 1074
Fsp4HI GCNGC 5 cut(s) 26, 29, 282, 306, 803
FspBI CTAG 3 cut(s) 42, 998, 1415
GlaI GCGC 2 cut(s) 567, 1164
GluI GCNGC 5 cut(s) 26, 29, 282, 306, 803
HaeIII GGCC 2 cut(s) 882, 1421
HapII CCGG 2 cut(s) 75, 1422
HgaI GACGC 1 cut(s) 1245
HhaI GCGC 2 cut(s) 568, 1165
Hin1II CATG 7 cut(s) 148, 167, 781, 1021, 1030, 1078, 1232
Hin6I GCGC 2 cut(s) 566, 1163
HinP1I GCGC 2 cut(s) 566, 1163
HincII GTYRAC 2 cut(s) 1333, 1378
HindII GTYRAC 2 cut(s) 1333, 1378
HinfI GANTC 4 cut(s) 265, 475, 1232, 1345
HpaI GTTAAC 1 cut(s) 1333
HpaII CCGG 2 cut(s) 75, 1422
HphI GGTGA 3 cut(s) 272, 881, 1060
Hpy166II GTNNAC 3 cut(s) 1318, 1333, 1378
Hpy188I TCNGA 3 cut(s) 226, 1223, 1358
Hpy188III TCNNGA 9 cut(s) 59, 412, 796, 964, 998, 1007, 1180, 1349, 1415
Hpy8I GTNNAC 3 cut(s) 1318, 1333, 1378
HpyAV CCTTC 5 cut(s) 126, 461, 693, 1094, 1107
HpyCH4III ACNGT 6 cut(s) 17, 293, 369, 418, 814, 995
HpyCH4IV ACGT 1 cut(s) 1322
HpyF10VI GCNNNNNNNGC 2 cut(s) 499, 1078
HpyF3I CTNAG 5 cut(s) 32, 285, 659, 921, 1361
HpySE526I ACGT 1 cut(s) 1322
Hsp92II CATG 7 cut(s) 148, 167, 781, 1021, 1030, 1078, 1232
HspAI GCGC 2 cut(s) 566, 1163
Ksp22I TGATCA 1 cut(s) 433
KspAI GTTAAC 1 cut(s) 1333
Lsp1109I GCAGC 4 cut(s) 37, 292, 293, 789
LweI GCATC 3 cut(s) 382, 957, 1100
MaeI CTAG 3 cut(s) 42, 998, 1415
MaeII ACGT 1 cut(s) 1322
MaeIII GTNAC 5 cut(s) 516, 838, 1117, 1257, 1323
MboII GAAGA 6 cut(s) 113, 197, 661, 741, 1022, 1397
MflI RGATCY 5 cut(s) 35, 221, 408, 909, 1411
MhlI GDGCHC 3 cut(s) 70, 706, 1074
MlsI TGGCCA 1 cut(s) 882
MluCI AATT 7 cut(s) 54, 393, 524, 819, 930, 1041, 1132
MluNI TGGCCA 1 cut(s) 882
MlyI GAGTC 3 cut(s) 259, 469, 1241
MmeI TCCRAC 4 cut(s) 613, 796, 1381, 1386
Mox20I TGGCCA 1 cut(s) 882
MscI TGGCCA 1 cut(s) 882
MseI TTAA 6 cut(s) 105, 198, 420, 638, 807, 1332
MslI CAYNNNNRTG 2 cut(s) 63, 1181
Msp20I TGGCCA 1 cut(s) 882
MspA1I CMGCKG 1 cut(s) 28
MspI CCGG 2 cut(s) 75, 1422
MspR9I CCNGG 3 cut(s) 762, 901, 1423
Mva1269I GAATGC 1 cut(s) 684
MvaI CCWGG 2 cut(s) 762, 901
MvnI CGCG 1 cut(s) 1337
MwoI GCNNNNNNNGC 2 cut(s) 499, 1078
NciI CCSGG 1 cut(s) 1423
NcoI CCATGG 1 cut(s) 777
NdeI CATATG 2 cut(s) 276, 741
NlaIII CATG 7 cut(s) 148, 167, 781, 1021, 1030, 1078, 1232
NlaIV GGNNCC 1 cut(s) 724
NmuCI GTSAC 2 cut(s) 1117, 1257
OliI CACNNNNGTG 1 cut(s) 63
PaeR7I CTCGAG 1 cut(s) 786
PcsI WCGNNNNNNNCGW 1 cut(s) 1095
PctI GAATGC 1 cut(s) 684
PfeI GAWTC 1 cut(s) 1345
PkrI GCNGC 5 cut(s) 27, 30, 283, 307, 804
PleI GAGTC 3 cut(s) 259, 469, 1240
PpsI GAGTC 3 cut(s) 259, 469, 1240
PshAI GACNNNNGTC 1 cut(s) 993
Psp6I CCWGG 2 cut(s) 760, 899
PspGI CCWGG 2 cut(s) 760, 899
PspN4I GGNNCC 1 cut(s) 724
PspPI GGNCC 2 cut(s) 484, 890
PspXI VCTCGAGB 1 cut(s) 786
PsrI GAACNNNNNNTAC 2 cut(s) 774, 806
PstNI CAGNNNCTG 1 cut(s) 31
PsuI RGATCY 5 cut(s) 35, 221, 408, 909, 1411
RsaI GTAC 4 cut(s) 389, 849, 856, 1396
RsaNI GTAC 4 cut(s) 388, 848, 855, 1395
RseI CAYNNNNRTG 2 cut(s) 63, 1181
SaqAI TTAA 6 cut(s) 105, 198, 420, 638, 807, 1332
SatI GCNGC 5 cut(s) 26, 29, 282, 306, 803
Sau96I GGNCC 2 cut(s) 484, 890
SchI GAGTC 3 cut(s) 259, 469, 1241
ScrFI CCNGG 3 cut(s) 762, 901, 1423
SduI GDGCHC 3 cut(s) 70, 706, 1074
SfaNI GCATC 3 cut(s) 382, 957, 1100
Sfr274I CTCGAG 1 cut(s) 786
SinI GGWCC 2 cut(s) 484, 890
SlaI CTCGAG 1 cut(s) 786
SmiMI CAYNNNNRTG 2 cut(s) 63, 1181
SmlI CTYRAG 5 cut(s) 320, 495, 569, 608, 786
SmoI CTYRAG 5 cut(s) 320, 495, 569, 608, 786
Sse9I AATT 7 cut(s) 54, 393, 524, 819, 930, 1041, 1132
SsiI CCGC 6 cut(s) 28, 39, 380, 532, 737, 977
SspI AATATT 2 cut(s) 628, 718
SspMI CTAG 3 cut(s) 42, 998, 1415
StyD4I CCNGG 3 cut(s) 760, 899, 1421
StyI CCWWGG 3 cut(s) 777, 1152, 1192
TaaI ACNGT 6 cut(s) 17, 293, 369, 418, 814, 995
TaiI ACGT 1 cut(s) 1325
TaqI TCGA 4 cut(s) 339, 413, 787, 1429
TasI AATT 7 cut(s) 54, 393, 524, 819, 930, 1041, 1132
TatI WGTACW 2 cut(s) 847, 1394
TauI GCSGC 1 cut(s) 31
TfiI GAWTC 1 cut(s) 1345
Tru1I TTAA 6 cut(s) 105, 198, 420, 638, 807, 1332
Tru9I TTAA 6 cut(s) 105, 198, 420, 638, 807, 1332
TscAI CASTG 6 cut(s) 432, 457, 514, 1153, 1266, 1387
TseFI GTSAC 2 cut(s) 1117, 1257
TseI GCWGC 4 cut(s) 25, 281, 305, 802
Tsp45I GTSAC 2 cut(s) 1117, 1257
TspGWI ACGGA 1 cut(s) 1295
TspRI CASTG 6 cut(s) 432, 457, 514, 1153, 1266, 1387
VpaK11BI GGWCC 2 cut(s) 484, 890
XagI CCTNNNNNAGG 1 cut(s) 765
XapI RAATTY 2 cut(s) 819, 930
XbaI TCTAGA 2 cut(s) 997, 1414
XhoI CTCGAG 1 cut(s) 786
XspI CTAG 3 cut(s) 42, 998, 1415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.