pycom02g03840

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0011301
Physical Location & Seq
Forward (+)
2555557 .. 2558018
2462 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g03840.1

Sequence Viewer

Length: 1044 bp
ATGCATAGGCGCCCAGATCAACGGTCGAGATCCGCCAAGCCCACTACCATCCACGGCTGCGCTCAGTCCGGCGACCTTATTGGGTTTCAGAAGCTGCTTCGGGAAAACCCATCTCTTCTCAACGAGAAAAATGCCGTTATGGCACAAACTCCTCTTCATGTTTCTGCTGGTTACAACAGGGGTGAGATAGTTAAATTTCTTCTTGATTGGCGAGGGCCGGGTGAAGTTGAGTTGGAAGCCAAGAACATGTACGGAGAAACACCATTGCACATGGCAGCAAAGAATGGATGCAGTGATGCTGCACGGTTGCTTCTTGTCCATGGTGCTTTTATCGAAGCCAAAGCAAATAACGGAATGACCCCATTACACCTTGCTGTGTGGTACTCACTTACCGCTGATGAGTTCTCAACTGTCAAAACATTACTAGAGTACAATGCTGATTGCAGTGCTAAGGACGATGAGGGCATGACTCCTATACATCATGTATCAAAAGGCCGTGCAAATGGAAAGCTGCGGGAACTATTACAGTGGCACTACGAAGAGCAGAGGAGAAAAAAGGCAATTGAAGCATGCAGTGAAACTAAAGCTAAGATGGATGAACTTGAAAGTGAGTTATCAAATATTGTTGGTTTGCATGAGCTCAAGGTACAACTACGGAAATGGGCAAAGGGTATGCTTTTGGATGAGAGGCGTAAAGCTCTTGGGCTGAAAGTTGGTGTAAGGAGACCGCCTCATATGGCCTTTCTTGGAAATCCCGGAACCGGTAAGACCATGGTAGCTCGAATTCTTGGAAGATTACTCCATATGGTGGGAATTCTACCTACTGATAAAGTAACAGAAGTACAGAGGACTGATTTGGTTGGGGAATTCGTTGGTCATACTGGACCCAAAACCAGGAGGAAGATTAAAGACGCAGAGGGAGGAATTCTTTTTGTAGATGAAGCTTATCGACTAATACCTATGCAGAAGGCAGACGATAAAGACTATGGCTTGGAAGCATTGGAAGATCGATCATGTCTGTCATGGATAGTGGAAAAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA Ank Ank_2 Ank_3

Protein Analysis

348

Amino Acids

38.85

Weight (kDa)

8.93

Isoelectric Point (pI)

33.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 18 - 114 1e-13 Ankyrin repeats (3 copies)
Ank_5 PF13857 78 - 125 8.1e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 82 - 152 1.7e-13 Ankyrin repeats (3 copies)
Ank PF00023 84 - 114 3.6e-06 Ankyrin repeat
Ank_4 PF13637 89 - 127 9.3e-06 Ankyrin repeats (many copies)
AAA PF00004 246 - 326 4.6e-09 ATPase family associated with various cellular activities (AAA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24530 AT3G24530 AT3G24530
fragaria_vesca FvH4_1g04710
malus_domestica MD02G1046000.v1.1 MD15G1184200.v1.1
prunus_persica Prupe.7G232000_v2.0.a1 Prupe.7G232000_v2.0.a1
pyrus_communis pycom02g03840 pycom02g03850 pycom15g16580
rosa_chinensis RchiOBHm_Chr2g0090311
rosa_laevigata RLG00000016097
rosa_multiflora Rmu_co8096142.1_g000001 Rmu_sc0006235.1_g000004
rosa_roxburghii Rroxscaffold_2G00151290
rosa_rugosa Rorug02G0006500
rosa_samantha Rh2CG053200 Rh2DG052300
rosa_wichuraiana Rw2G004450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 9
AccB7I CCANNNNNTGG 1 cut(s) 808
AciI CCGC 4 cut(s) 33, 393, 514, 728
AclWI GGATC 1 cut(s) 24
AcsI RAATTY 5 cut(s) 194, 783, 813, 866, 924
AcyI GRCGYC 1 cut(s) 10
AfaI GTAC 5 cut(s) 251, 383, 431, 648, 843
AfiI CCNNNNNNNGG 3 cut(s) 761, 808, 894
AflIII ACRYGT 1 cut(s) 246
AgeI ACCGGT 1 cut(s) 761
AgsI TTSAA 2 cut(s) 566, 605
AjnI CCWGG 1 cut(s) 893
AluBI AGCT 7 cut(s) 94, 511, 587, 640, 698, 779, 944
AluI AGCT 7 cut(s) 94, 511, 587, 640, 698, 779, 944
Alw21I GWGCWC 1 cut(s) 642
Alw26I GTCTC 1 cut(s) 718
AlwI GGATC 1 cut(s) 24
AlwNI CAGNNNCTG 1 cut(s) 94
AoxI GGCC 3 cut(s) 215, 493, 738
ApeKI GCWGC 5 cut(s) 57, 94, 275, 299, 511
ApoI RAATTY 5 cut(s) 194, 783, 813, 866, 924
AsiGI ACCGGT 1 cut(s) 761
AspLEI GCGC 2 cut(s) 12, 62
AspS9I GGNCC 2 cut(s) 215, 884
AsuC2I CCSGG 2 cut(s) 219, 756
AsuHPI GGTGA 2 cut(s) 194, 233
AvaII GGWCC 1 cut(s) 884
BanI GGYRCC 1 cut(s) 9
BanII GRGCYC 1 cut(s) 642
Bbv12I GWGCWC 1 cut(s) 642
BbvI GCAGC 5 cut(s) 44, 81, 286, 287, 498
BccI CCATC 3 cut(s) 56, 118, 586
BceAI ACGGC 3 cut(s) 70, 119, 480
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciT130I CCWGG 1 cut(s) 895
BcnI CCSGG 2 cut(s) 219, 756
BcoDI GTCTC 1 cut(s) 718
BfaI CTAG 1 cut(s) 425
BfoI RGCGCY 1 cut(s) 13
BglI GCCNNNNNGGC 1 cut(s) 140
BisI GCNGC 5 cut(s) 58, 95, 276, 300, 512
BlsI GCNGC 5 cut(s) 59, 96, 277, 301, 513
Bme1390I CCNGG 3 cut(s) 219, 756, 895
Bme18I GGWCC 1 cut(s) 884
BmgT120I GGNCC 2 cut(s) 215, 884
BmiI GGNNCC 3 cut(s) 11, 760, 886
BmrFI CCNGG 3 cut(s) 219, 756, 895
BmsI GCATC 2 cut(s) 278, 286
BplI GAGNNNNNCTC 2 cut(s) 715, 747
Bpu10I CCTNAGC 1 cut(s) 450
BpuEI CTTGAG 1 cut(s) 626
BpuMI CCSGG 2 cut(s) 219, 756
Bsa29I ATCGAT 1 cut(s) 1009
BsaHI GRCGYC 1 cut(s) 10
BsaI GGTCTC 1 cut(s) 718
BsaJI CCNNGG 3 cut(s) 52, 319, 771
BsaWI WCCGGW 1 cut(s) 761
Bsc4I CCNNNNNNNGG 3 cut(s) 761, 808, 894
Bse118I RCCGGY 1 cut(s) 761
Bse1I ACTGG 1 cut(s) 886
Bse3DI GCAATG 1 cut(s) 263
BseBI CCWGG 1 cut(s) 895
BseCI ATCGAT 1 cut(s) 1009
BseDI CCNNGG 3 cut(s) 52, 319, 771
BseGI GGATG 4 cut(s) 48, 293, 601, 688
BseLI CCNNNNNNNGG 3 cut(s) 761, 808, 894
BseMI GCAATG 1 cut(s) 263
BseMII CTCAG 1 cut(s) 77
BseNI ACTGG 1 cut(s) 886
BseRI GAGGAG 2 cut(s) 141, 562
BseXI GCAGC 5 cut(s) 44, 81, 286, 287, 498
BsgI GTGCAG 1 cut(s) 285
Bsh1285I CGRYCG 1 cut(s) 26
BshFI GGCC 3 cut(s) 217, 495, 740
BshNI GGYRCC 1 cut(s) 9
BshTI ACCGGT 1 cut(s) 761
BshVI ATCGAT 1 cut(s) 1009
BsiEI CGRYCG 1 cut(s) 26
BsiHKAI GWGCWC 1 cut(s) 642
BsiSI CCGG 4 cut(s) 69, 218, 756, 762
BslI CCNNNNNNNGG 3 cut(s) 761, 808, 894
BsmAI GTCTC 1 cut(s) 718
BsnI GGCC 3 cut(s) 217, 495, 740
Bso31I GGTCTC 1 cut(s) 718
Bsp1286I GDGCHC 1 cut(s) 642
Bsp143I GATC 4 cut(s) 16, 29, 1006, 1010
Bsp19I CCATGG 2 cut(s) 319, 771
BspACI CCGC 4 cut(s) 33, 393, 514, 728
BspANI GGCC 3 cut(s) 217, 495, 740
BspCNI CTCAG 1 cut(s) 76
BspDI ATCGAT 1 cut(s) 1009
BspLI GGNNCC 3 cut(s) 11, 760, 886
BspPI GGATC 1 cut(s) 24
BspQI GCTCTTC 1 cut(s) 534
BspT107I GGYRCC 1 cut(s) 9
BspTNI GGTCTC 1 cut(s) 718
BsrDI GCAATG 1 cut(s) 263
BsrFI RCCGGY 1 cut(s) 761
BsrI ACTGG 1 cut(s) 886
BssAI RCCGGY 1 cut(s) 761
BssECI CCNNGG 3 cut(s) 52, 319, 771
BssMI GATC 4 cut(s) 16, 29, 1006, 1010
BssNI GRCGYC 1 cut(s) 10
BssT1I CCWWGG 2 cut(s) 319, 771
Bst2UI CCWGG 1 cut(s) 895
Bst4CI ACNGT 4 cut(s) 24, 306, 412, 528
Bst6I CTCTTC 3 cut(s) 120, 159, 534
BstACI GRCGYC 1 cut(s) 10
BstC8I GCNNGC 1 cut(s) 571
BstDEI CTNAG 3 cut(s) 63, 450, 588
BstDSI CCRYGG 3 cut(s) 52, 319, 771
BstF5I GGATG 4 cut(s) 48, 293, 601, 688
BstH2I RGCGCY 1 cut(s) 13
BstHHI GCGC 2 cut(s) 12, 62
BstKTI GATC 4 cut(s) 19, 32, 1009, 1013
BstMAI GTCTC 1 cut(s) 718
BstMBI GATC 4 cut(s) 16, 29, 1006, 1010
BstMCI CGRYCG 1 cut(s) 26
BstMWI GCNNNNNNNGC 2 cut(s) 140, 566
BstNI CCWGG 1 cut(s) 895
BstNSI RCATGY 2 cut(s) 250, 573
BstSCI CCNGG 3 cut(s) 217, 754, 893
BstV1I GCAGC 5 cut(s) 44, 81, 286, 287, 498
BstX2I RGATCY 1 cut(s) 29
BstYI RGATCY 1 cut(s) 29
Bsu15I ATCGAT 1 cut(s) 1009
BsuRI GGCC 3 cut(s) 217, 495, 740
BsuTUI ATCGAT 1 cut(s) 1009
BtgI CCRYGG 3 cut(s) 52, 319, 771
BtsCI GGATG 4 cut(s) 48, 293, 601, 688
BtsI GCAGTG 3 cut(s) 298, 451, 580
BtsIMutI CAGTG 4 cut(s) 298, 451, 533, 580
Cac8I GCNNGC 1 cut(s) 571
CaiI CAGNNNCTG 1 cut(s) 94
CfoI GCGC 2 cut(s) 12, 62
Cfr10I RCCGGY 1 cut(s) 761
Cfr13I GGNCC 2 cut(s) 215, 884
ClaI ATCGAT 1 cut(s) 1009
CseI GACGC 1 cut(s) 920
Csp6I GTAC 5 cut(s) 250, 382, 430, 647, 842
CspAI ACCGGT 1 cut(s) 761
CviQI GTAC 5 cut(s) 250, 382, 430, 647, 842
DdeI CTNAG 3 cut(s) 63, 450, 588
DinI GGCGCC 1 cut(s) 11
DpnI GATC 4 cut(s) 18, 31, 1008, 1012
DpnII GATC 4 cut(s) 16, 29, 1006, 1010
Eam1104I CTCTTC 3 cut(s) 120, 159, 534
EarI CTCTTC 3 cut(s) 120, 159, 534
EciI GGCGGA 1 cut(s) 22
Ecl136II GAGCTC 1 cut(s) 640
Eco130I CCWWGG 2 cut(s) 319, 771
Eco24I GRGCYC 1 cut(s) 642
Eco31I GGTCTC 1 cut(s) 718
Eco47I GGWCC 1 cut(s) 884
Eco53kI GAGCTC 1 cut(s) 640
EcoICRI GAGCTC 1 cut(s) 640
EcoRI GAATTC 4 cut(s) 783, 813, 866, 924
EcoRII CCWGG 1 cut(s) 893
EcoT14I CCWWGG 2 cut(s) 319, 771
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 642
EgeI GGCGCC 1 cut(s) 11
EheI GGCGCC 1 cut(s) 11
ErhI CCWWGG 2 cut(s) 319, 771
FauI CCCGC 1 cut(s) 507
FauNDI CATATG 2 cut(s) 735, 804
Fnu4HI GCNGC 5 cut(s) 58, 95, 276, 300, 512
FokI GGATG 4 cut(s) 35, 300, 608, 695
FriOI GRGCYC 1 cut(s) 642
Fsp4HI GCNGC 5 cut(s) 58, 95, 276, 300, 512
FspBI CTAG 1 cut(s) 425
GlaI GCGC 2 cut(s) 11, 61
GluI GCNGC 5 cut(s) 58, 95, 276, 300, 512
HaeII RGCGCY 1 cut(s) 13
HaeIII GGCC 3 cut(s) 217, 495, 740
HapII CCGG 4 cut(s) 69, 218, 756, 762
HgaI GACGC 1 cut(s) 920
HhaI GCGC 2 cut(s) 12, 62
Hin1I GRCGYC 1 cut(s) 10
Hin6I GCGC 2 cut(s) 10, 60
HinP1I GCGC 2 cut(s) 10, 60
HindIII AAGCTT 1 cut(s) 942
HinfI GANTC 1 cut(s) 469
HpaII CCGG 4 cut(s) 69, 218, 756, 762
HphI GGTGA 2 cut(s) 194, 233
Hpy188I TCNGA 1 cut(s) 90
Hpy188III TCNNGA 3 cut(s) 27, 101, 203
HpyAV CCTTC 1 cut(s) 961
HpyCH4III ACNGT 4 cut(s) 24, 306, 412, 528
HpyCH4V TGCA 9 cut(s) 4, 268, 291, 302, 444, 500, 573, 634, 964
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 566
HpyF3I CTNAG 3 cut(s) 63, 450, 588
Hsp92I GRCGYC 1 cut(s) 10
HspAI GCGC 2 cut(s) 10, 60
KasI GGCGCC 1 cut(s) 9
Kzo9I GATC 4 cut(s) 16, 29, 1006, 1010
LguI GCTCTTC 1 cut(s) 534
Lsp1109I GCAGC 5 cut(s) 44, 81, 286, 287, 498
LweI GCATC 2 cut(s) 278, 286
MaeI CTAG 1 cut(s) 425
MaeIII GTNAC 2 cut(s) 170, 832
MalI GATC 4 cut(s) 18, 31, 1008, 1012
MboI GATC 4 cut(s) 16, 29, 1006, 1010
MboII GAAGA 7 cut(s) 107, 146, 191, 551, 804, 913, 1016
MfeI CAATTG 1 cut(s) 561
MflI RGATCY 1 cut(s) 29
MhlI GDGCHC 1 cut(s) 642
MluCI AATT 7 cut(s) 194, 561, 783, 813, 866, 924, 1037
Mly113I GGCGCC 1 cut(s) 10
MlyI GAGTC 1 cut(s) 463
MmeI TCCRAC 1 cut(s) 213
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 2 cut(s) 192, 906
MspA1I CMGCKG 1 cut(s) 395
MspI CCGG 4 cut(s) 69, 218, 756, 762
MspR9I CCNGG 3 cut(s) 219, 756, 895
MunI CAATTG 1 cut(s) 561
MvaI CCWGG 1 cut(s) 895
MwoI GCNNNNNNNGC 2 cut(s) 140, 566
NarI GGCGCC 1 cut(s) 10
NciI CCSGG 2 cut(s) 219, 756
NcoI CCATGG 2 cut(s) 319, 771
NdeI CATATG 2 cut(s) 735, 804
NdeII GATC 4 cut(s) 16, 29, 1006, 1010
NlaIV GGNNCC 3 cut(s) 11, 760, 886
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 250, 573
PaeI GCATGC 1 cut(s) 573
PciI ACATGT 1 cut(s) 246
PciSI GCTCTTC 1 cut(s) 534
PflMI CCANNNNNTGG 1 cut(s) 808
PfoI TCCNGGA 1 cut(s) 754
PinAI ACCGGT 1 cut(s) 761
PkrI GCNGC 5 cut(s) 59, 96, 277, 301, 513
PleI GAGTC 1 cut(s) 463
PluTI GGCGCC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 463
PscI ACATGT 1 cut(s) 246
Psp124BI GAGCTC 1 cut(s) 642
Psp6I CCWGG 1 cut(s) 893
PspGI CCWGG 1 cut(s) 893
PspN4I GGNNCC 3 cut(s) 11, 760, 886
PspPI GGNCC 2 cut(s) 215, 884
PstNI CAGNNNCTG 1 cut(s) 94
PsuI RGATCY 1 cut(s) 29
RsaI GTAC 5 cut(s) 251, 383, 431, 648, 843
RsaNI GTAC 5 cut(s) 250, 382, 430, 647, 842
SacI GAGCTC 1 cut(s) 642
SapI GCTCTTC 1 cut(s) 534
SaqAI TTAA 2 cut(s) 192, 906
SatI GCNGC 5 cut(s) 58, 95, 276, 300, 512
Sau3AI GATC 4 cut(s) 16, 29, 1006, 1010
Sau96I GGNCC 2 cut(s) 215, 884
SchI GAGTC 1 cut(s) 463
ScrFI CCNGG 3 cut(s) 219, 756, 895
SduI GDGCHC 1 cut(s) 642
SfaNI GCATC 2 cut(s) 278, 286
SfoI GGCGCC 1 cut(s) 11
SinI GGWCC 1 cut(s) 884
SmlI CTYRAG 1 cut(s) 641
SmoI CTYRAG 1 cut(s) 641
SphI GCATGC 1 cut(s) 573
Sse9I AATT 7 cut(s) 194, 561, 783, 813, 866, 924, 1037
SsiI CCGC 4 cut(s) 33, 393, 514, 728
SspDI GGCGCC 1 cut(s) 9
SspI AATATT 1 cut(s) 622
SspMI CTAG 1 cut(s) 425
SstI GAGCTC 1 cut(s) 642
StyD4I CCNGG 3 cut(s) 217, 754, 893
StyI CCWWGG 2 cut(s) 319, 771
TaaI ACNGT 4 cut(s) 24, 306, 412, 528
TaqI TCGA 5 cut(s) 26, 333, 781, 949, 1009
TasI AATT 7 cut(s) 194, 561, 783, 813, 866, 924, 1037
TatI WGTACW 2 cut(s) 429, 841
Tru1I TTAA 2 cut(s) 192, 906
Tru9I TTAA 2 cut(s) 192, 906
TscAI CASTG 4 cut(s) 298, 451, 533, 580
TseI GCWGC 5 cut(s) 57, 94, 275, 299, 511
TspDTI ATGAA 3 cut(s) 146, 612, 954
TspGWI ACGGA 3 cut(s) 267, 366, 670
TspRI CASTG 4 cut(s) 298, 451, 533, 580
Van91I CCANNNNNTGG 1 cut(s) 808
VpaK11BI GGWCC 1 cut(s) 884
XapI RAATTY 5 cut(s) 194, 783, 813, 866, 924
XceI RCATGY 2 cut(s) 250, 573
XspI CTAG 1 cut(s) 425
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.