Rh2CG053200

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0011301
Physical Location & Seq
Forward (+)
4202658 .. 4206096
3439 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG053200.1

Sequence Viewer

Length: 1308 bp
ATGCAGATGGTACAAACTCCACTTCATGTTTCTGCTGGTAATAATAGGTCTGACATAGTTAAATTTCTTCTCAATTGGCAAGGGCCGGAAAAGGTTGAGTTGGAAGCCAGAAACATGTATGGTGAAACACCATTACACATGGCAGCAAAGAATGGATGCAATGATGCTGCAAAGTTGCTTCTTGTCCATGGTTCTTTTATTGAAGCCAAAGCAAATAATGGAATGACACCATTACACCTTGCTGTTTGGCATTCACTTCGAGCTGAAGATTTCTCAACTGTTAAAACATTACTTGAGAACAATGCTGATTGCAGTGCCAAGGACGATGAGGGCATGACTCCTTTAAATCACCTTTCAAAAGGCCATGAAACTGAAAAGTTGCGGGAACTATTACAGTGGCACCTTGAAGAGCAGACAAAGAAAAGGGCAATTCAAGCATGCAGTGAAACTAAAGCTAAGATGGATGAACTTGAAAATGAATTATCAAAAATAGTTGGTTTGCAAGAGCTCAAATTACAACTACGCAAGTGGGCAAAAGGGATGCTTTTGGATGAGAGGCGTAAGGCCCTTGGTCTCAAAGTTGGCATAAGGAGACCCCCTCATATGGCCTTTCTTGGCAATCCCGGAACAGGTAAGACCATGGTAGCTCGTATTCTTGGAAGATTACTCTATATGGTGGGAATTCTACCTACTGACAAAGTAACTGAAGTACAGCGGACAGATTTGGTTGGGGAATTTGTTGGTCATACGGGACCAAAAACTAGGAGGAAGATTAAGGATGCAGAGGGAGGAATTCTTTTTGTAGATGAAGCTTATCGACTGATACCTATGCAGAAGGCAGATGATAAAGACTATGGGTTGGAAGCATTGGAAGAAATCATGTCTGTCATGGACAGTGGAAAGATCGTAGTCATATTTGCTGGATATAGTGAACCAATGAAACGTGTAATTGCTTCGAATGAAGGTTTCTGCAGACGTGTGACCAAGTTTTTCAACTTTAGTGACTTCAGTCCTGAAGATCTAGCAAAGATTCTTCATCTCAAGATGAATAATCAAACAGAGGAGAGCATGTTGTATGGATTTAAGCTACATTCTTTTTGCAGTTTAGAAGCCATTGCTGAACTGATTCAAAGAGGAGCAACAGAAAAGCAGTGTAAGGAGATGAATGGAGGTTTAGTGGATACAATGTTAGTTAATGCTAGAGAGAATTTGGACCTTAGGCTCAATTTTGATTGTATAGACACAGAAGAACTTAGAACAATCACCTTAGAGGATTTACAAGCAGGCCTTCAGATTTTGTCACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA Ank Ank_2 Ank_3

Protein Analysis

435

Amino Acids

48.98

Weight (kDa)

6.22

Isoelectric Point (pI)

32.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 8 - 108 2.4e-17 Ankyrin repeats (3 copies)
Ank_5 PF13857 34 - 81 4e-07 Ankyrin repeats (many copies)
Ank_4 PF13637 45 - 85 2.2e-06 Ankyrin repeats (many copies)
AAA PF00004 203 - 318 3.2e-12 ATPase family associated with various cellular activities (AAA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24530 AT3G24530 AT3G24530
fragaria_vesca FvH4_1g04710
malus_domestica MD02G1046000.v1.1 MD15G1184200.v1.1
prunus_persica Prupe.7G232000_v2.0.a1 Prupe.7G232000_v2.0.a1
pyrus_communis pycom02g03840 pycom02g03850 pycom15g16580
rosa_chinensis RchiOBHm_Chr2g0090311
rosa_laevigata RLG00000016097
rosa_multiflora Rmu_co8096142.1_g000001 Rmu_sc0006235.1_g000004
rosa_roxburghii Rroxscaffold_2G00151290
rosa_rugosa Rorug02G0006500
rosa_samantha Rh2CG053200 Rh2DG052300
rosa_wichuraiana Rw2G004450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 399
AciI CCGC 2 cut(s) 382, 715
AcsI RAATTY 5 cut(s) 62, 681, 734, 792, 1207
AcuI CTGAAG 5 cut(s) 285, 726, 991, 1035, 1274
AfaI GTAC 2 cut(s) 12, 711
AfiI CCNNNNNNNGG 2 cut(s) 604, 629
AflIII ACRYGT 3 cut(s) 114, 943, 976
AgsI TTSAA 7 cut(s) 203, 357, 407, 434, 473, 994, 1130
AjiI CACGTC 1 cut(s) 977
AluBI AGCT 6 cut(s) 263, 455, 508, 647, 812, 1087
AluI AGCT 6 cut(s) 263, 455, 508, 647, 812, 1087
Alw21I GWGCWC 1 cut(s) 510
Alw26I GTCTC 2 cut(s) 578, 586
AoxI GGCC 5 cut(s) 83, 361, 564, 606, 1285
ApeKI GCWGC 2 cut(s) 143, 167
ApoI RAATTY 5 cut(s) 62, 681, 734, 792, 1207
Asp700I GAANNNNTTC 1 cut(s) 1125
AspS9I GGNCC 4 cut(s) 83, 565, 752, 1213
AsuC2I CCSGG 1 cut(s) 624
AsuHPI GGTGA 3 cut(s) 134, 341, 1255
AsuII TTCGAA 1 cut(s) 956
AvaII GGWCC 2 cut(s) 752, 1213
AxyI CCTNAGG 1 cut(s) 1217
BanI GGYRCC 1 cut(s) 399
BanII GRGCYC 1 cut(s) 510
Bbv12I GWGCWC 1 cut(s) 510
BbvI GCAGC 2 cut(s) 154, 155
BccI CCATC 1 cut(s) 454
BcgI CGANNNNNNTGC 2 cut(s) 239, 273
BciVI GTATCC 1 cut(s) 1174
BcnI CCSGG 1 cut(s) 624
BcoDI GTCTC 2 cut(s) 578, 586
BfaI CTAG 3 cut(s) 762, 1022, 1200
BfmI CTRYAG 1 cut(s) 970
BfuI GTATCC 1 cut(s) 1174
BglII AGATCT 1 cut(s) 1018
BisI GCNGC 2 cut(s) 144, 168
BlsI GCNGC 2 cut(s) 145, 169
Bme1390I CCNGG 1 cut(s) 624
Bme18I GGWCC 2 cut(s) 752, 1213
BmgBI CACGTC 1 cut(s) 977
BmgT120I GGNCC 4 cut(s) 83, 565, 752, 1213
BmiI GGNNCC 2 cut(s) 401, 753
BmrFI CCNGG 1 cut(s) 624
BmsI GCATC 4 cut(s) 146, 154, 531, 769
BoxI GACNNNNGTC 1 cut(s) 1008
BplI GAGNNNNNCTC 2 cut(s) 583, 615
Bpu14I TTCGAA 1 cut(s) 956
BpuEI CTTGAG 2 cut(s) 314, 1025
BpuMI CCSGG 1 cut(s) 624
BsaI GGTCTC 2 cut(s) 578, 586
BsaJI CCNNGG 4 cut(s) 187, 318, 568, 639
BsaXI ACNNNNNCTCC 4 cut(s) 1055, 1085, 1161, 1191
Bsc4I CCNNNNNNNGG 2 cut(s) 604, 629
Bse21I CCTNAGG 1 cut(s) 1217
Bse3DI GCAATG 2 cut(s) 166, 1113
BseDI CCNNGG 4 cut(s) 187, 318, 568, 639
BseGI GGATG 5 cut(s) 161, 469, 546, 556, 784
BseLI CCNNNNNNNGG 2 cut(s) 604, 629
BseMI GCAATG 2 cut(s) 166, 1113
BseRI GAGGAG 2 cut(s) 1076, 1149
BseXI GCAGC 2 cut(s) 154, 155
BshFI GGCC 5 cut(s) 85, 363, 566, 608, 1287
BshNI GGYRCC 1 cut(s) 399
BsiHKAI GWGCWC 1 cut(s) 510
BsiSI CCGG 2 cut(s) 86, 624
BslFI GGGAC 1 cut(s) 765
BslI CCNNNNNNNGG 2 cut(s) 604, 629
BsmAI GTCTC 2 cut(s) 578, 586
BsmFI GGGAC 1 cut(s) 765
BsmI GAATGC 1 cut(s) 250
BsnI GGCC 5 cut(s) 85, 363, 566, 608, 1287
Bso31I GGTCTC 2 cut(s) 578, 586
Bsp119I TTCGAA 1 cut(s) 956
Bsp1286I GDGCHC 1 cut(s) 510
Bsp143I GATC 2 cut(s) 903, 1018
Bsp19I CCATGG 2 cut(s) 187, 639
BspACI CCGC 2 cut(s) 382, 715
BspANI GGCC 5 cut(s) 85, 363, 566, 608, 1287
BspLI GGNNCC 2 cut(s) 401, 753
BspMAI CTGCAG 1 cut(s) 974
BspQI GCTCTTC 1 cut(s) 402
BspT104I TTCGAA 1 cut(s) 956
BspT107I GGYRCC 1 cut(s) 399
BspTNI GGTCTC 2 cut(s) 578, 586
BsrDI GCAATG 2 cut(s) 166, 1113
BssECI CCNNGG 4 cut(s) 187, 318, 568, 639
BssMI GATC 2 cut(s) 903, 1018
BssT1I CCWWGG 4 cut(s) 187, 318, 568, 639
Bst4CI ACNGT 3 cut(s) 280, 396, 896
Bst6I CTCTTC 1 cut(s) 402
BstBI TTCGAA 1 cut(s) 956
BstC8I GCNNGC 2 cut(s) 439, 1285
BstDEI CTNAG 4 cut(s) 456, 1217, 1253, 1267
BstDSI CCRYGG 2 cut(s) 187, 639
BstF5I GGATG 5 cut(s) 161, 469, 546, 556, 784
BstKTI GATC 2 cut(s) 906, 1021
BstMAI GTCTC 2 cut(s) 578, 586
BstMBI GATC 2 cut(s) 903, 1018
BstMWI GCNNNNNNNGC 1 cut(s) 434
BstNSI RCATGY 3 cut(s) 118, 441, 1072
BstPAI GACNNNNGTC 1 cut(s) 1008
BstSCI CCNGG 1 cut(s) 622
BstSFI CTRYAG 1 cut(s) 970
BstV1I GCAGC 2 cut(s) 154, 155
BstX2I RGATCY 1 cut(s) 1018
BstYI RGATCY 1 cut(s) 1018
Bsu36I CCTNAGG 1 cut(s) 1217
BsuI GTATCC 1 cut(s) 1174
BsuRI GGCC 5 cut(s) 85, 363, 566, 608, 1287
BtgI CCRYGG 2 cut(s) 187, 639
BtrI CACGTC 1 cut(s) 977
BtsCI GGATG 5 cut(s) 161, 469, 546, 556, 784
BtsI GCAGTG 3 cut(s) 319, 448, 1157
BtsIMutI CAGTG 5 cut(s) 319, 401, 448, 901, 1157
Cac8I GCNNGC 2 cut(s) 439, 1285
Cfr13I GGNCC 4 cut(s) 83, 565, 752, 1213
Csp6I GTAC 2 cut(s) 11, 710
CviQI GTAC 2 cut(s) 11, 710
DdeI CTNAG 4 cut(s) 456, 1217, 1253, 1267
DpnI GATC 2 cut(s) 905, 1020
DpnII GATC 2 cut(s) 903, 1018
DraI TTTAAA 1 cut(s) 345
Eam1104I CTCTTC 1 cut(s) 402
EarI CTCTTC 1 cut(s) 402
Ecl136II GAGCTC 1 cut(s) 508
Eco130I CCWWGG 4 cut(s) 187, 318, 568, 639
Eco147I AGGCCT 1 cut(s) 1287
Eco24I GRGCYC 1 cut(s) 510
Eco31I GGTCTC 2 cut(s) 578, 586
Eco47I GGWCC 2 cut(s) 752, 1213
Eco53kI GAGCTC 1 cut(s) 508
Eco57I CTGAAG 5 cut(s) 285, 726, 991, 1035, 1274
Eco81I CCTNAGG 1 cut(s) 1217
EcoICRI GAGCTC 1 cut(s) 508
EcoO109I RGGNCCY 1 cut(s) 565
EcoRI GAATTC 2 cut(s) 681, 792
EcoT14I CCWWGG 4 cut(s) 187, 318, 568, 639
EcoT38I GRGCYC 1 cut(s) 510
ErhI CCWWGG 4 cut(s) 187, 318, 568, 639
FalI AAGNNNNNCTT 4 cut(s) 528, 560, 1272, 1304
FaqI GGGAC 1 cut(s) 765
FauI CCCGC 1 cut(s) 375
FauNDI CATATG 1 cut(s) 603
Fnu4HI GCNGC 2 cut(s) 144, 168
FokI GGATG 5 cut(s) 168, 476, 553, 563, 791
FriOI GRGCYC 1 cut(s) 510
Fsp4HI GCNGC 2 cut(s) 144, 168
FspBI CTAG 3 cut(s) 762, 1022, 1200
GluI GCNGC 2 cut(s) 144, 168
HaeIII GGCC 5 cut(s) 85, 363, 566, 608, 1287
HapII CCGG 2 cut(s) 86, 624
HindIII AAGCTT 1 cut(s) 810
HinfI GANTC 3 cut(s) 337, 1030, 1126
HpaII CCGG 2 cut(s) 86, 624
HphI GGTGA 3 cut(s) 134, 341, 1255
Hpy166II GTNNAC 1 cut(s) 932
Hpy188I TCNGA 2 cut(s) 52, 1293
Hpy188III TCNNGA 2 cut(s) 1013, 1042
Hpy8I GTNNAC 1 cut(s) 932
HpyAV CCTTC 3 cut(s) 829, 956, 1298
HpyCH4III ACNGT 3 cut(s) 280, 396, 896
HpyCH4IV ACGT 2 cut(s) 943, 976
HpyF10VI GCNNNNNNNGC 1 cut(s) 434
HpyF3I CTNAG 4 cut(s) 456, 1217, 1253, 1267
HpySE526I ACGT 2 cut(s) 943, 976
Kzo9I GATC 2 cut(s) 903, 1018
LguI GCTCTTC 1 cut(s) 402
LmnI GCTCC 1 cut(s) 1136
LpnPI CCDG 8 cut(s) 21, 99, 121, 615, 637, 906, 1026, 1269
Lsp1109I GCAGC 2 cut(s) 154, 155
LweI GCATC 4 cut(s) 146, 154, 531, 769
MaeI CTAG 3 cut(s) 762, 1022, 1200
MaeII ACGT 2 cut(s) 943, 976
MaeIII GTNAC 4 cut(s) 700, 979, 1001, 1299
MalI GATC 2 cut(s) 905, 1020
MboI GATC 2 cut(s) 903, 1018
MboII GAAGA 9 cut(s) 59, 278, 419, 672, 781, 884, 1025, 1028, 1259
MfeI CAATTG 1 cut(s) 73
MflI RGATCY 1 cut(s) 1018
MhlI GDGCHC 1 cut(s) 510
MlyI GAGTC 1 cut(s) 331
MmeI TCCRAC 2 cut(s) 81, 840
MroXI GAANNNNTTC 1 cut(s) 1125
MseI TTAA 6 cut(s) 60, 282, 344, 774, 1083, 1194
MspA1I CMGCKG 1 cut(s) 715
MspI CCGG 2 cut(s) 86, 624
MspR9I CCNGG 1 cut(s) 624
MunI CAATTG 1 cut(s) 73
Mva1269I GAATGC 1 cut(s) 250
MwoI GCNNNNNNNGC 1 cut(s) 434
NciI CCSGG 1 cut(s) 624
NcoI CCATGG 2 cut(s) 187, 639
NdeI CATATG 1 cut(s) 603
NdeII GATC 2 cut(s) 903, 1018
NlaIV GGNNCC 2 cut(s) 401, 753
NmuCI GTSAC 3 cut(s) 979, 1001, 1299
NspI RCATGY 3 cut(s) 118, 441, 1072
NspV TTCGAA 1 cut(s) 956
PaeI GCATGC 1 cut(s) 441
PceI AGGCCT 1 cut(s) 1287
PciI ACATGT 1 cut(s) 114
PciSI GCTCTTC 1 cut(s) 402
PctI GAATGC 1 cut(s) 250
PdmI GAANNNNTTC 1 cut(s) 1125
PfeI GAWTC 2 cut(s) 1030, 1126
PfoI TCCNGGA 1 cut(s) 622
PkrI GCNGC 2 cut(s) 145, 169
PleI GAGTC 1 cut(s) 331
PpsI GAGTC 1 cut(s) 331
PscI ACATGT 1 cut(s) 114
PshAI GACNNNNGTC 1 cut(s) 1008
Psp124BI GAGCTC 1 cut(s) 510
PspN4I GGNNCC 2 cut(s) 401, 753
PspPI GGNCC 4 cut(s) 83, 565, 752, 1213
PstI CTGCAG 1 cut(s) 974
PsuI RGATCY 1 cut(s) 1018
RsaI GTAC 2 cut(s) 12, 711
RsaNI GTAC 2 cut(s) 11, 710
SacI GAGCTC 1 cut(s) 510
SapI GCTCTTC 1 cut(s) 402
SaqAI TTAA 6 cut(s) 60, 282, 344, 774, 1083, 1194
SatI GCNGC 2 cut(s) 144, 168
Sau3AI GATC 2 cut(s) 903, 1018
Sau96I GGNCC 4 cut(s) 83, 565, 752, 1213
SchI GAGTC 1 cut(s) 331
ScrFI CCNGG 1 cut(s) 624
SduI GDGCHC 1 cut(s) 510
SfaNI GCATC 4 cut(s) 146, 154, 531, 769
SfcI CTRYAG 1 cut(s) 970
SfuI TTCGAA 1 cut(s) 956
SinI GGWCC 2 cut(s) 752, 1213
SmlI CTYRAG 2 cut(s) 293, 1040
SmoI CTYRAG 2 cut(s) 293, 1040
SphI GCATGC 1 cut(s) 441
SseBI AGGCCT 1 cut(s) 1287
SsiI CCGC 2 cut(s) 382, 715
SspMI CTAG 3 cut(s) 762, 1022, 1200
SstI GAGCTC 1 cut(s) 510
StuI AGGCCT 1 cut(s) 1287
StyD4I CCNGG 1 cut(s) 622
StyI CCWWGG 4 cut(s) 187, 318, 568, 639
TaaI ACNGT 3 cut(s) 280, 396, 896
TaiI ACGT 2 cut(s) 946, 979
TaqI TCGA 3 cut(s) 259, 817, 956
TatI WGTACW 1 cut(s) 709
TfiI GAWTC 2 cut(s) 1030, 1126
Tru1I TTAA 6 cut(s) 60, 282, 344, 774, 1083, 1194
Tru9I TTAA 6 cut(s) 60, 282, 344, 774, 1083, 1194
TscAI CASTG 5 cut(s) 319, 401, 448, 901, 1157
TseFI GTSAC 3 cut(s) 979, 1001, 1299
TseI GCWGC 2 cut(s) 143, 167
Tsp45I GTSAC 3 cut(s) 979, 1001, 1299
TspRI CASTG 5 cut(s) 319, 401, 448, 901, 1157
VpaK11BI GGWCC 2 cut(s) 752, 1213
XapI RAATTY 5 cut(s) 62, 681, 734, 792, 1207
XceI RCATGY 3 cut(s) 118, 441, 1072
XmnI GAANNNNTTC 1 cut(s) 1125
XspI CTAG 3 cut(s) 762, 1022, 1200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.