FvH4_1g04710

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0011301
Physical Location & Seq
Forward (+)
2487071 .. 2490366
3296 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g04710.t1

Sequence Viewer

Length: 1440 bp
ATGCAGAGGAACCCAGATCAACGGTCGAGATCCGCCAAGCCCACCACCATCCACGGCTACGCTCACTCCGGCGACCTTTCTGGGTTTCAGAAGCTGCTCCGAGAAAACCCAGCTCTGCTCAATGAAAGAAATGCCATTATGGTACAAACTCCGCTTCATGTTTCTGCTGGTAACAATAGGTCTGAGATAGTTAAATTTCTTCTTGATTGGCAAGGGCCGGAAAAGGTTGAGCTGGAAGCCAGAAACATGTATGGTGAAACACCATTACACATGGCAGCAAAGAATGGATGCAACGATGCTGCACAGTTGCTTCTTGTCCATGGTGCTTCCATTGAAGCCAAAGCAAATAATGGAATGACACCGTTACACCTTGCTGTTTGGCATTCACTTCGAGCTGAAGATTTCTCAACTGTTAAAACATTGCTTGAGAACAATGCTGATTGTAGTGCCAAGGACGATGAAGGCATGACTCCTCTAAATCACATTCCGAAAGGCCGTGAAACTGAAAAGTTGCGGGAACTATTACAGTGGCACGTTGAAGAGCAGACAAAAAGAAGGGCAATACAAGCATGCACTGAAACTAAAGCTAAGATGGATGAACTAGAAAATGAATTGTCAAATATAGTTGGTTTGCAAGATCTCAAAGTACAACTACGGAAGTGGGCCAAAGGGATGCTTTTGGATGAGAGGCGTAAGGCCCTTGGTCTCAAAGTTGGTGTAAGGAGACCCCCTCATATGGCCTTCCTTGGCAATCCTGGAACAGGTAAGACCATGGTAGCTCGTATTCTTGGAAGATTACTTTATATGGTGGGAATTCTACCAACTGATAAAGTAACTGAAGTACAGCGGACAGATTTGGTTGGGGAATTTGTTGGTCATACGGGACCAAAAACTAGGAGGAAGATTAAAGATGCAGAGGGAGGAATTCTTTTTGTAGATGAAGCTTATCGACTGATACCTATGCAGAAGGCAGATGATAAAGACTATGGGTTGGAAGCATTGGAAGAGATCATGTCTGTCATGGATAGTGGAAAGATTGTAGTTATATTTGCTGGATATAGTGAACCAATGAAACGTGTAATTGCTTCCAATGAAGGTTTCTGCCGACGAGTGACCAAGTTTTTCAACTTTAGTGACTTCAGTCCTGAAGATCTAGCAAAGATTCTTCATCTCAAGATGAATAATCAACCAGAGGAGAGCATGTTGTATGGATTCAAACTACATTCTTCTTGCAGTTTAGAAGCTATTGCAGAACTGATTCGAAGAGGAGCAACAGAAAAGCAGTGTAAGGAGATGAATGGAGGTTTAGTGGATACAATGTTAGTTAATGCTAGAGAGAGTTTGGACCTTAGACTCAATTTTGATTGTATAGACACAGAAGAACTTAGAACAATCACCTTAGAGGATTTACAAGCAGGCCTTCAGATATTGTCACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA Ank Ank_2 Ank_3

Protein Analysis

480

Amino Acids

53.77

Weight (kDa)

6.53

Isoelectric Point (pI)

41.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 18 - 114 6.2e-14 Ankyrin repeats (3 copies)
Ank_5 PF13857 78 - 125 8.9e-09 Ankyrin repeats (many copies)
Ank_2 PF12796 84 - 152 8.2e-13 Ankyrin repeats (3 copies)
Ank PF00023 84 - 114 7.9e-07 Ankyrin repeat
Ank_4 PF13637 89 - 129 4.5e-07 Ankyrin repeats (many copies)
AAA PF00004 247 - 362 3.8e-12 ATPase family associated with various cellular activities (AAA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24530 AT3G24530 AT3G24530
fragaria_vesca FvH4_1g04710
malus_domestica MD02G1046000.v1.1 MD15G1184200.v1.1
prunus_persica Prupe.7G232000_v2.0.a1 Prupe.7G232000_v2.0.a1
pyrus_communis pycom02g03840 pycom02g03850 pycom15g16580
rosa_chinensis RchiOBHm_Chr2g0090311
rosa_laevigata RLG00000016097
rosa_multiflora Rmu_co8096142.1_g000001 Rmu_sc0006235.1_g000004
rosa_roxburghii Rroxscaffold_2G00151290
rosa_rugosa Rorug02G0006500
rosa_samantha Rh2CG053200 Rh2DG052300
rosa_wichuraiana Rw2G004450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 33, 152, 514, 847
AclWI GGATC 1 cut(s) 24
AcsI RAATTY 4 cut(s) 194, 813, 866, 924
AcuI CTGAAG 5 cut(s) 417, 858, 1123, 1167, 1406
AfaI GTAC 3 cut(s) 144, 648, 843
AfiI CCNNNNNNNGG 2 cut(s) 736, 761
AflIII ACRYGT 2 cut(s) 246, 1075
AgsI TTSAA 4 cut(s) 335, 539, 1126, 1216
AjnI CCWGG 1 cut(s) 754
AjuI GAANNNNNNNTTGG 2 cut(s) 1082, 1114
AluBI AGCT 8 cut(s) 94, 113, 232, 395, 587, 779, 944, 1244
AluI AGCT 8 cut(s) 94, 113, 232, 395, 587, 779, 944, 1244
Alw26I GTCTC 2 cut(s) 710, 718
AlwI GGATC 1 cut(s) 24
AlwNI CAGNNNCTG 1 cut(s) 94
AoxI GGCC 6 cut(s) 215, 493, 663, 696, 738, 1417
ApeKI GCWGC 3 cut(s) 94, 275, 299
ApoI RAATTY 4 cut(s) 194, 813, 866, 924
Asp700I GAANNNNTTC 1 cut(s) 1257
AspS9I GGNCC 5 cut(s) 215, 663, 697, 884, 1345
AsuHPI GGTGA 2 cut(s) 266, 1387
AsuII TTCGAA 1 cut(s) 1261
AvaII GGWCC 2 cut(s) 884, 1345
BbvI GCAGC 3 cut(s) 81, 286, 287
BccI CCATC 2 cut(s) 56, 586
BceAI ACGGC 2 cut(s) 70, 480
BcgI CGANNNNNNTGC 2 cut(s) 371, 405
BciT130I CCWGG 1 cut(s) 756
BciVI GTATCC 1 cut(s) 1306
BcoDI GTCTC 2 cut(s) 710, 718
BfaI CTAG 4 cut(s) 602, 894, 1154, 1332
BfuI GTATCC 1 cut(s) 1306
BglII AGATCT 2 cut(s) 637, 1150
BisI GCNGC 3 cut(s) 95, 276, 300
BlsI GCNGC 3 cut(s) 96, 277, 301
Bme1390I CCNGG 1 cut(s) 756
Bme18I GGWCC 2 cut(s) 884, 1345
BmgT120I GGNCC 5 cut(s) 215, 663, 697, 884, 1345
BmiI GGNNCC 2 cut(s) 11, 885
BmrFI CCNGG 1 cut(s) 756
BmsI GCATC 4 cut(s) 278, 286, 663, 901
BoxI GACNNNNGTC 1 cut(s) 1140
BplI GAGNNNNNCTC 2 cut(s) 715, 747
Bpu14I TTCGAA 1 cut(s) 1261
BpuEI CTTGAG 2 cut(s) 446, 1157
BsaI GGTCTC 2 cut(s) 710, 718
BsaJI CCNNGG 6 cut(s) 52, 319, 450, 700, 745, 771
BsaXI ACNNNNNCTCC 6 cut(s) 50, 80, 1187, 1217, 1293, 1323
Bsc4I CCNNNNNNNGG 2 cut(s) 736, 761
Bse3DI GCAATG 1 cut(s) 419
BseBI CCWGG 1 cut(s) 756
BseDI CCNNGG 6 cut(s) 52, 319, 450, 700, 745, 771
BseGI GGATG 5 cut(s) 48, 293, 601, 678, 688
BseLI CCNNNNNNNGG 2 cut(s) 736, 761
BseMI GCAATG 1 cut(s) 419
BseMII CTCAG 1 cut(s) 174
BseRI GAGGAG 3 cut(s) 462, 1208, 1281
BseXI GCAGC 3 cut(s) 81, 286, 287
BseYI CCCAGC 1 cut(s) 109
BsgI GTGCAG 1 cut(s) 285
Bsh1285I CGRYCG 1 cut(s) 26
BshFI GGCC 6 cut(s) 217, 495, 665, 698, 740, 1419
BsiEI CGRYCG 1 cut(s) 26
BsiSI CCGG 2 cut(s) 69, 218
BslFI GGGAC 1 cut(s) 897
BslI CCNNNNNNNGG 2 cut(s) 736, 761
BsmAI GTCTC 2 cut(s) 710, 718
BsmFI GGGAC 1 cut(s) 897
BsmI GAATGC 1 cut(s) 382
BsnI GGCC 6 cut(s) 217, 495, 665, 698, 740, 1419
Bso31I GGTCTC 2 cut(s) 710, 718
Bsp119I TTCGAA 1 cut(s) 1261
Bsp143I GATC 5 cut(s) 16, 29, 637, 1008, 1150
Bsp19I CCATGG 2 cut(s) 319, 771
BspACI CCGC 4 cut(s) 33, 152, 514, 847
BspANI GGCC 6 cut(s) 217, 495, 665, 698, 740, 1419
BspCNI CTCAG 1 cut(s) 175
BspLI GGNNCC 2 cut(s) 11, 885
BspPI GGATC 1 cut(s) 24
BspQI GCTCTTC 1 cut(s) 534
BspT104I TTCGAA 1 cut(s) 1261
BspTNI GGTCTC 2 cut(s) 710, 718
BsrDI GCAATG 1 cut(s) 419
BssECI CCNNGG 6 cut(s) 52, 319, 450, 700, 745, 771
BssMI GATC 5 cut(s) 16, 29, 637, 1008, 1150
BssT1I CCWWGG 5 cut(s) 319, 450, 700, 745, 771
Bst2UI CCWGG 1 cut(s) 756
Bst4CI ACNGT 5 cut(s) 24, 306, 363, 412, 528
Bst6I CTCTTC 3 cut(s) 534, 999, 1258
BstBI TTCGAA 1 cut(s) 1261
BstC8I GCNNGC 2 cut(s) 571, 1417
BstDEI CTNAG 5 cut(s) 183, 588, 1349, 1385, 1399
BstDSI CCRYGG 3 cut(s) 52, 319, 771
BstENI CCTNNNNNAGG 1 cut(s) 759
BstF5I GGATG 5 cut(s) 48, 293, 601, 678, 688
BstKTI GATC 5 cut(s) 19, 32, 640, 1011, 1153
BstMAI GTCTC 2 cut(s) 710, 718
BstMBI GATC 5 cut(s) 16, 29, 637, 1008, 1150
BstMCI CGRYCG 1 cut(s) 26
BstMWI GCNNNNNNNGC 1 cut(s) 566
BstNI CCWGG 1 cut(s) 756
BstNSI RCATGY 3 cut(s) 250, 573, 1204
BstPAI GACNNNNGTC 1 cut(s) 1140
BstSCI CCNGG 1 cut(s) 754
BstV1I GCAGC 3 cut(s) 81, 286, 287
BstX2I RGATCY 3 cut(s) 29, 637, 1150
BstYI RGATCY 3 cut(s) 29, 637, 1150
BsuI GTATCC 1 cut(s) 1306
BsuRI GGCC 6 cut(s) 217, 495, 665, 698, 740, 1419
BtgI CCRYGG 3 cut(s) 52, 319, 771
BtsCI GGATG 5 cut(s) 48, 293, 601, 678, 688
BtsI GCAGTG 1 cut(s) 1289
BtsIMutI CAGTG 3 cut(s) 533, 573, 1289
Cac8I GCNNGC 2 cut(s) 571, 1417
CaiI CAGNNNCTG 1 cut(s) 94
Cfr13I GGNCC 5 cut(s) 215, 663, 697, 884, 1345
Csp6I GTAC 3 cut(s) 143, 647, 842
CviQI GTAC 3 cut(s) 143, 647, 842
DdeI CTNAG 5 cut(s) 183, 588, 1349, 1385, 1399
DpnI GATC 5 cut(s) 18, 31, 639, 1010, 1152
DpnII GATC 5 cut(s) 16, 29, 637, 1008, 1150
Eam1104I CTCTTC 3 cut(s) 534, 999, 1258
EarI CTCTTC 3 cut(s) 534, 999, 1258
EciI GGCGGA 1 cut(s) 22
Eco130I CCWWGG 5 cut(s) 319, 450, 700, 745, 771
Eco147I AGGCCT 1 cut(s) 1419
Eco31I GGTCTC 2 cut(s) 710, 718
Eco47I GGWCC 2 cut(s) 884, 1345
Eco57I CTGAAG 5 cut(s) 417, 858, 1123, 1167, 1406
EcoNI CCTNNNNNAGG 1 cut(s) 759
EcoO109I RGGNCCY 1 cut(s) 697
EcoRI GAATTC 2 cut(s) 813, 924
EcoRII CCWGG 1 cut(s) 754
EcoT14I CCWWGG 5 cut(s) 319, 450, 700, 745, 771
ErhI CCWWGG 5 cut(s) 319, 450, 700, 745, 771
FalI AAGNNNNNCTT 4 cut(s) 660, 692, 1404, 1436
FaqI GGGAC 1 cut(s) 897
FauI CCCGC 1 cut(s) 507
FauNDI CATATG 1 cut(s) 735
Fnu4HI GCNGC 3 cut(s) 95, 276, 300
FokI GGATG 5 cut(s) 35, 300, 608, 685, 695
Fsp4HI GCNGC 3 cut(s) 95, 276, 300
FspBI CTAG 4 cut(s) 602, 894, 1154, 1332
GluI GCNGC 3 cut(s) 95, 276, 300
GsaI CCCAGC 1 cut(s) 113
HaeIII GGCC 6 cut(s) 217, 495, 665, 698, 740, 1419
HapII CCGG 2 cut(s) 69, 218
HindIII AAGCTT 1 cut(s) 942
HinfI GANTC 5 cut(s) 469, 1162, 1212, 1258, 1353
HpaII CCGG 2 cut(s) 69, 218
HphI GGTGA 2 cut(s) 266, 1387
Hpy166II GTNNAC 1 cut(s) 1064
Hpy188I TCNGA 5 cut(s) 90, 101, 184, 489, 1425
Hpy188III TCNNGA 4 cut(s) 27, 203, 1145, 1174
Hpy8I GTNNAC 1 cut(s) 1064
Hpy99I CGWCG 1 cut(s) 1110
HpyAV CCTTC 6 cut(s) 455, 549, 751, 961, 1088, 1430
HpyCH4III ACNGT 5 cut(s) 24, 306, 363, 412, 528
HpyCH4IV ACGT 2 cut(s) 534, 1075
HpyCH4V TGCA 9 cut(s) 4, 291, 302, 573, 634, 914, 964, 1233, 1250
HpyF10VI GCNNNNNNNGC 1 cut(s) 566
HpyF3I CTNAG 5 cut(s) 183, 588, 1349, 1385, 1399
HpySE526I ACGT 2 cut(s) 534, 1075
Kzo9I GATC 5 cut(s) 16, 29, 637, 1008, 1150
LguI GCTCTTC 1 cut(s) 534
LmnI GCTCC 2 cut(s) 102, 1268
Lsp1109I GCAGC 3 cut(s) 81, 286, 287
LweI GCATC 4 cut(s) 278, 286, 663, 901
MaeI CTAG 4 cut(s) 602, 894, 1154, 1332
MaeII ACGT 2 cut(s) 534, 1075
MaeIII GTNAC 6 cut(s) 170, 363, 832, 1111, 1133, 1431
MalI GATC 5 cut(s) 18, 31, 639, 1010, 1152
MboI GATC 5 cut(s) 16, 29, 637, 1008, 1150
MflI RGATCY 3 cut(s) 29, 637, 1150
MluCI AATT 7 cut(s) 194, 611, 813, 866, 924, 1080, 1357
MlyI GAGTC 2 cut(s) 463, 1347
MmeI TCCRAC 1 cut(s) 972
MroXI GAANNNNTTC 1 cut(s) 1257
MseI TTAA 4 cut(s) 192, 414, 906, 1326
MspA1I CMGCKG 1 cut(s) 847
MspI CCGG 2 cut(s) 69, 218
MspR9I CCNGG 1 cut(s) 756
Mva1269I GAATGC 1 cut(s) 382
MvaI CCWGG 1 cut(s) 756
MwoI GCNNNNNNNGC 1 cut(s) 566
NcoI CCATGG 2 cut(s) 319, 771
NdeI CATATG 1 cut(s) 735
NdeII GATC 5 cut(s) 16, 29, 637, 1008, 1150
NlaIV GGNNCC 2 cut(s) 11, 885
NmuCI GTSAC 3 cut(s) 1111, 1133, 1431
NspI RCATGY 3 cut(s) 250, 573, 1204
NspV TTCGAA 1 cut(s) 1261
PaeI GCATGC 1 cut(s) 573
PceI AGGCCT 1 cut(s) 1419
PciI ACATGT 1 cut(s) 246
PciSI GCTCTTC 1 cut(s) 534
PctI GAATGC 1 cut(s) 382
PdmI GAANNNNTTC 1 cut(s) 1257
PfeI GAWTC 3 cut(s) 1162, 1212, 1258
PfoI TCCNGGA 1 cut(s) 754
PkrI GCNGC 3 cut(s) 96, 277, 301
PleI GAGTC 2 cut(s) 463, 1347
PpsI GAGTC 2 cut(s) 463, 1347
PscI ACATGT 1 cut(s) 246
PshAI GACNNNNGTC 1 cut(s) 1140
Psp6I CCWGG 1 cut(s) 754
PspFI CCCAGC 1 cut(s) 109
PspGI CCWGG 1 cut(s) 754
PspN4I GGNNCC 2 cut(s) 11, 885
PspPI GGNCC 5 cut(s) 215, 663, 697, 884, 1345
PstNI CAGNNNCTG 1 cut(s) 94
PsuI RGATCY 3 cut(s) 29, 637, 1150
RsaI GTAC 3 cut(s) 144, 648, 843
RsaNI GTAC 3 cut(s) 143, 647, 842
SapI GCTCTTC 1 cut(s) 534
SaqAI TTAA 4 cut(s) 192, 414, 906, 1326
SatI GCNGC 3 cut(s) 95, 276, 300
Sau3AI GATC 5 cut(s) 16, 29, 637, 1008, 1150
Sau96I GGNCC 5 cut(s) 215, 663, 697, 884, 1345
SchI GAGTC 2 cut(s) 463, 1347
ScrFI CCNGG 1 cut(s) 756
SfaNI GCATC 4 cut(s) 278, 286, 663, 901
SfuI TTCGAA 1 cut(s) 1261
SinI GGWCC 2 cut(s) 884, 1345
SmlI CTYRAG 2 cut(s) 425, 1172
SmoI CTYRAG 2 cut(s) 425, 1172
SphI GCATGC 1 cut(s) 573
Sse9I AATT 7 cut(s) 194, 611, 813, 866, 924, 1080, 1357
SseBI AGGCCT 1 cut(s) 1419
SsiI CCGC 4 cut(s) 33, 152, 514, 847
SspMI CTAG 4 cut(s) 602, 894, 1154, 1332
StuI AGGCCT 1 cut(s) 1419
StyD4I CCNGG 1 cut(s) 754
StyI CCWWGG 5 cut(s) 319, 450, 700, 745, 771
TaaI ACNGT 5 cut(s) 24, 306, 363, 412, 528
TaiI ACGT 2 cut(s) 537, 1078
TaqI TCGA 4 cut(s) 26, 391, 949, 1261
TasI AATT 7 cut(s) 194, 611, 813, 866, 924, 1080, 1357
TatI WGTACW 2 cut(s) 646, 841
TfiI GAWTC 3 cut(s) 1162, 1212, 1258
Tru1I TTAA 4 cut(s) 192, 414, 906, 1326
Tru9I TTAA 4 cut(s) 192, 414, 906, 1326
TscAI CASTG 3 cut(s) 533, 580, 1289
TseFI GTSAC 3 cut(s) 1111, 1133, 1431
TseI GCWGC 3 cut(s) 94, 275, 299
Tsp45I GTSAC 3 cut(s) 1111, 1133, 1431
TspGWI ACGGA 1 cut(s) 670
TspRI CASTG 3 cut(s) 533, 580, 1289
VpaK11BI GGWCC 2 cut(s) 884, 1345
XagI CCTNNNNNAGG 1 cut(s) 759
XapI RAATTY 4 cut(s) 194, 813, 866, 924
XceI RCATGY 3 cut(s) 250, 573, 1204
XmnI GAANNNNTTC 1 cut(s) 1257
XspI CTAG 4 cut(s) 602, 894, 1154, 1332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.