pycom15g16580

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0011301
Physical Location & Seq
Forward (+)
11622164 .. 11624981
2818 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g16580.7

Sequence Viewer

Length: 1440 bp
ATGCAGAGGCGCCCAGATCAACGGTCGAGATCCGCCAGGCCCACCACCATCCACGGCTTCGCTCAGTCCGGCGACCTTCTTGGCTTCCAGAAGCTGCTTCGGGAAACCCCATCTCTTCTCAACGAGAAAAATGCCGTTATGCAACAAACTCCACTTCATGTTTCTGCTGGTTACAACAGGGGCGACATAGTTAAATTCCTTCTTGATTGGCAAGGGCCGGGTGAAGTTGAGTTGGAAGCCAAGAACATGTACGGAGAAACACCATTGCACATGGCAGCAAAGAATGGATGCAATGATGCTGCACGGTTGCTTCTTGTCCATGGTGCTTTTATCGAAGCCAAGGCAAATAATGGAATGACACCACTACACCTTGCTGTGTGGTACTCACTTACAGCTGAAGAGTTCTCAACTGTCAAAACATTACTAGAGTACAATGCTGATTGCAGTGCTAAGGACGATGAGGGCATGACTCCTATACATCACTTATCTAAGGGCCGTGCAAATGAAAAGTTGCGGGAACTATTACAGTGGCACTATGAAGAGCAGAGGAGAAAAAAAGCAATTGAAGCATGCAGTGAAACTAAAGCTAAGATGGATGAACTTGAAAGTGAGTTATCAAATATTGTTGGTTTGCATGAGCTCAAGGTACAACTACGGAAATGGGCAAAGGGGATGCTTTTGGATGAGAGGCGTAAGGCCCTTGGGCTGAAAGTTGGTGTGAGGAGACCGCCTCATATGGCTTTTCTTGGAAATCCTGGAACTGGTAAGACCATGGTAGCTCGAATTCTTGGAAGATTACTCCATATGGTAGGAATTCTACCTACTGATAAAGTAACAGAAGTACAGAGGACCGATTTGGTTGGGGAATTCGTTGGTCACACTGGACCCAAAACTAGGAGGAAGATTAAAGACGCAGAGGGAGGAATCCTTTTTGTAGATGAAGCTTATCGACTAATACCCATGCAGAAGGCAGACGATAAAGACTATGGCTTGGAAGCATTGGAAGAGATCATGTCTGTCATGGATAGTGGAAAAATCGTAGTCATATTTGCAGGATATAGCGAACCAATGAAACGTGTAATTGCTTCAAATGAAGGTTTCTGTCGACGTGTGACCAAGTTTTTCAATTTTAGTGACTTCACTTCAGAAGACCTAGCAAGGATCCTCCATATCAAGATGCATAATCAGACAGAGGAGAGCTTGTTGTATGGATTTCAGTTACACTCTTCGTGCAGTATAGAAGCAGTTGCAGATCTGATTCGAAGAGAGACAACAGAAAAGCAGTGCAAGGAGATGAATGGAGGTTTAGTGGATACGATGTTAGTGAATGCTAGAGAGAGTTTGGATTTTAGGCTCGATTTTGATTGTATAGATACAGACGAACTTAGAACAATCACCCTAGAGGATTTACAAGCAGGCCTTCGGGTTTTGTCACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA Ank Ank_2 Ank_3

Protein Analysis

480

Amino Acids

54.03

Weight (kDa)

6.46

Isoelectric Point (pI)

40.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24530 AT3G24530 AT3G24530
fragaria_vesca FvH4_1g04710
malus_domestica MD02G1046000.v1.1 MD15G1184200.v1.1
prunus_persica Prupe.7G232000_v2.0.a1 Prupe.7G232000_v2.0.a1
pyrus_communis pycom02g03840 pycom02g03850 pycom15g16580
rosa_chinensis RchiOBHm_Chr2g0090311
rosa_laevigata RLG00000016097
rosa_multiflora Rmu_co8096142.1_g000001 Rmu_sc0006235.1_g000004
rosa_roxburghii Rroxscaffold_2G00151290
rosa_rugosa Rorug02G0006500
rosa_samantha Rh2CG053200 Rh2DG052300
rosa_wichuraiana Rw2G004450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 9
AccI GTMKAC 1 cut(s) 1105
AciI CCGC 3 cut(s) 33, 514, 728
AclWI GGATC 3 cut(s) 24, 1156, 1169
AcsI RAATTY 4 cut(s) 194, 783, 813, 866
AcuI CTGAAG 2 cut(s) 417, 1128
AcyI GRCGYC 1 cut(s) 10
AfaI GTAC 5 cut(s) 251, 383, 431, 648, 843
AfiI CCNNNNNNNGG 2 cut(s) 761, 894
AflIII ACRYGT 3 cut(s) 246, 1075, 1108
AgsI TTSAA 4 cut(s) 566, 605, 1089, 1126
AjiI CACGTC 1 cut(s) 1109
AjnI CCWGG 2 cut(s) 35, 754
AluBI AGCT 7 cut(s) 94, 395, 587, 640, 779, 944, 1200
AluI AGCT 7 cut(s) 94, 395, 587, 640, 779, 944, 1200
Alw21I GWGCWC 1 cut(s) 642
Alw26I GTCTC 2 cut(s) 718, 1262
AlwI GGATC 3 cut(s) 24, 1156, 1169
AlwNI CAGNNNCTG 1 cut(s) 94
AoxI GGCC 5 cut(s) 38, 215, 493, 696, 1417
ApeKI GCWGC 3 cut(s) 94, 275, 299
ApoI RAATTY 4 cut(s) 194, 783, 813, 866
AspLEI GCGC 1 cut(s) 12
AspS9I GGNCC 6 cut(s) 39, 215, 493, 697, 849, 884
AsuC2I CCSGG 1 cut(s) 219
AsuHPI GGTGA 2 cut(s) 233, 1387
AsuII TTCGAA 1 cut(s) 1261
AvaII GGWCC 2 cut(s) 849, 884
BamHI GGATCC 1 cut(s) 1161
BanI GGYRCC 1 cut(s) 9
BanII GRGCYC 1 cut(s) 642
BbsI GAAGAC 1 cut(s) 1155
Bbv12I GWGCWC 1 cut(s) 642
BbvI GCAGC 3 cut(s) 81, 286, 287
BccI CCATC 3 cut(s) 56, 118, 586
BceAI ACGGC 3 cut(s) 70, 119, 480
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciT130I CCWGG 2 cut(s) 37, 756
BciVI GTATCC 1 cut(s) 1306
BcnI CCSGG 1 cut(s) 219
BcoDI GTCTC 2 cut(s) 718, 1262
BfaI CTAG 5 cut(s) 425, 894, 1154, 1332, 1400
BfoI RGCGCY 1 cut(s) 13
BfuI GTATCC 1 cut(s) 1306
BglII AGATCT 1 cut(s) 1252
BisI GCNGC 3 cut(s) 95, 276, 300
BlsI GCNGC 3 cut(s) 96, 277, 301
Bme1390I CCNGG 3 cut(s) 37, 219, 756
Bme18I GGWCC 2 cut(s) 849, 884
BmgBI CACGTC 1 cut(s) 1109
BmgT120I GGNCC 6 cut(s) 39, 215, 493, 697, 849, 884
BmiI GGNNCC 3 cut(s) 11, 886, 1163
BmrFI CCNGG 3 cut(s) 37, 219, 756
BmsI GCATC 4 cut(s) 278, 286, 663, 1167
BpiI GAAGAC 1 cut(s) 1155
BplI GAGNNNNNCTC 2 cut(s) 715, 747
Bpu10I CCTNAGC 1 cut(s) 450
Bpu14I TTCGAA 1 cut(s) 1261
BpuEI CTTGAG 1 cut(s) 626
BpuMI CCSGG 1 cut(s) 219
BsaHI GRCGYC 1 cut(s) 10
BsaI GGTCTC 1 cut(s) 718
BsaJI CCNNGG 5 cut(s) 52, 319, 339, 700, 771
BsaXI ACNNNNNCTCC 4 cut(s) 1187, 1217, 1293, 1323
Bsc4I CCNNNNNNNGG 2 cut(s) 761, 894
Bse1I ACTGG 2 cut(s) 766, 886
Bse3DI GCAATG 2 cut(s) 263, 298
BseBI CCWGG 2 cut(s) 37, 756
BseDI CCNNGG 5 cut(s) 52, 319, 339, 700, 771
BseGI GGATG 5 cut(s) 48, 293, 601, 678, 688
BseLI CCNNNNNNNGG 2 cut(s) 761, 894
BseMI GCAATG 2 cut(s) 263, 298
BseMII CTCAG 1 cut(s) 77
BseNI ACTGG 2 cut(s) 766, 886
BseRI GAGGAG 3 cut(s) 562, 736, 1208
BseXI GCAGC 3 cut(s) 81, 286, 287
BsgI GTGCAG 2 cut(s) 285, 1252
Bsh1285I CGRYCG 1 cut(s) 26
BshFI GGCC 5 cut(s) 40, 217, 495, 698, 1419
BshNI GGYRCC 1 cut(s) 9
BsiEI CGRYCG 1 cut(s) 26
BsiHKAI GWGCWC 1 cut(s) 642
BsiSI CCGG 2 cut(s) 69, 218
BslI CCNNNNNNNGG 2 cut(s) 761, 894
BsmAI GTCTC 2 cut(s) 718, 1262
BsmI GAATGC 1 cut(s) 1333
BsnI GGCC 5 cut(s) 40, 217, 495, 698, 1419
Bso31I GGTCTC 1 cut(s) 718
Bsp119I TTCGAA 1 cut(s) 1261
Bsp1286I GDGCHC 1 cut(s) 642
Bsp143I GATC 5 cut(s) 16, 29, 1008, 1161, 1252
Bsp19I CCATGG 2 cut(s) 319, 771
BspACI CCGC 3 cut(s) 33, 514, 728
BspANI GGCC 5 cut(s) 40, 217, 495, 698, 1419
BspCNI CTCAG 1 cut(s) 76
BspLI GGNNCC 3 cut(s) 11, 886, 1163
BspPI GGATC 3 cut(s) 24, 1156, 1169
BspQI GCTCTTC 1 cut(s) 534
BspT104I TTCGAA 1 cut(s) 1261
BspT107I GGYRCC 1 cut(s) 9
BspTNI GGTCTC 1 cut(s) 718
BsrDI GCAATG 2 cut(s) 263, 298
BsrI ACTGG 2 cut(s) 766, 886
BssECI CCNNGG 5 cut(s) 52, 319, 339, 700, 771
BssMI GATC 5 cut(s) 16, 29, 1008, 1161, 1252
BssNI GRCGYC 1 cut(s) 10
BssT1I CCWWGG 4 cut(s) 319, 339, 700, 771
Bst2UI CCWGG 2 cut(s) 37, 756
Bst4CI ACNGT 5 cut(s) 24, 306, 412, 528, 1437
Bst6I CTCTTC 6 cut(s) 120, 393, 534, 999, 1231, 1258
BstACI GRCGYC 1 cut(s) 10
BstBI TTCGAA 1 cut(s) 1261
BstC8I GCNNGC 2 cut(s) 571, 1417
BstDEI CTNAG 5 cut(s) 63, 450, 489, 588, 1385
BstDSI CCRYGG 3 cut(s) 52, 319, 771
BstF5I GGATG 5 cut(s) 48, 293, 601, 678, 688
BstH2I RGCGCY 1 cut(s) 13
BstHHI GCGC 1 cut(s) 12
BstKTI GATC 5 cut(s) 19, 32, 1011, 1164, 1255
BstMAI GTCTC 2 cut(s) 718, 1262
BstMBI GATC 5 cut(s) 16, 29, 1008, 1161, 1252
BstMCI CGRYCG 1 cut(s) 26
BstMWI GCNNNNNNNGC 1 cut(s) 566
BstNI CCWGG 2 cut(s) 37, 756
BstNSI RCATGY 2 cut(s) 250, 573
BstSCI CCNGG 3 cut(s) 35, 217, 754
BstV1I GCAGC 3 cut(s) 81, 286, 287
BstV2I GAAGAC 1 cut(s) 1155
BstX2I RGATCY 3 cut(s) 29, 1161, 1252
BstYI RGATCY 3 cut(s) 29, 1161, 1252
BsuI GTATCC 1 cut(s) 1306
BsuRI GGCC 5 cut(s) 40, 217, 495, 698, 1419
BtgI CCRYGG 3 cut(s) 52, 319, 771
BtrI CACGTC 1 cut(s) 1109
BtsCI GGATG 5 cut(s) 48, 293, 601, 678, 688
BtsI GCAGTG 3 cut(s) 451, 580, 1289
BtsIMutI CAGTG 5 cut(s) 451, 533, 580, 879, 1289
Cac8I GCNNGC 2 cut(s) 571, 1417
CaiI CAGNNNCTG 1 cut(s) 94
CfoI GCGC 1 cut(s) 12
Cfr13I GGNCC 6 cut(s) 39, 215, 493, 697, 849, 884
CseI GACGC 1 cut(s) 920
Csp6I GTAC 5 cut(s) 250, 382, 430, 647, 842
CviQI GTAC 5 cut(s) 250, 382, 430, 647, 842
DdeI CTNAG 5 cut(s) 63, 450, 489, 588, 1385
DinI GGCGCC 1 cut(s) 11
DpnI GATC 5 cut(s) 18, 31, 1010, 1163, 1254
DpnII GATC 5 cut(s) 16, 29, 1008, 1161, 1252
Eam1104I CTCTTC 6 cut(s) 120, 393, 534, 999, 1231, 1258
EarI CTCTTC 6 cut(s) 120, 393, 534, 999, 1231, 1258
EciI GGCGGA 1 cut(s) 22
Ecl136II GAGCTC 1 cut(s) 640
Eco130I CCWWGG 4 cut(s) 319, 339, 700, 771
Eco147I AGGCCT 1 cut(s) 1419
Eco24I GRGCYC 1 cut(s) 642
Eco31I GGTCTC 1 cut(s) 718
Eco47I GGWCC 2 cut(s) 849, 884
Eco53kI GAGCTC 1 cut(s) 640
Eco57I CTGAAG 2 cut(s) 417, 1128
EcoICRI GAGCTC 1 cut(s) 640
EcoO109I RGGNCCY 1 cut(s) 697
EcoRI GAATTC 3 cut(s) 783, 813, 866
EcoRII CCWGG 2 cut(s) 35, 754
EcoT14I CCWWGG 4 cut(s) 319, 339, 700, 771
EcoT22I ATGCAT 1 cut(s) 1182
EcoT38I GRGCYC 1 cut(s) 642
EgeI GGCGCC 1 cut(s) 11
EheI GGCGCC 1 cut(s) 11
ErhI CCWWGG 4 cut(s) 319, 339, 700, 771
FalI AAGNNNNNCTT 2 cut(s) 1404, 1436
FauI CCCGC 1 cut(s) 507
FauNDI CATATG 2 cut(s) 735, 804
FblI GTMKAC 1 cut(s) 1105
Fnu4HI GCNGC 3 cut(s) 95, 276, 300
FokI GGATG 5 cut(s) 35, 300, 608, 685, 695
FriOI GRGCYC 1 cut(s) 642
Fsp4HI GCNGC 3 cut(s) 95, 276, 300
FspBI CTAG 5 cut(s) 425, 894, 1154, 1332, 1400
GlaI GCGC 1 cut(s) 11
GluI GCNGC 3 cut(s) 95, 276, 300
HaeII RGCGCY 1 cut(s) 13
HaeIII GGCC 5 cut(s) 40, 217, 495, 698, 1419
HapII CCGG 2 cut(s) 69, 218
HgaI GACGC 1 cut(s) 920
HhaI GCGC 1 cut(s) 12
Hin1I GRCGYC 1 cut(s) 10
Hin6I GCGC 1 cut(s) 10
HinP1I GCGC 1 cut(s) 10
HincII GTYRAC 1 cut(s) 1106
HindII GTYRAC 1 cut(s) 1106
HindIII AAGCTT 1 cut(s) 942
HinfI GANTC 3 cut(s) 469, 924, 1258
HpaII CCGG 2 cut(s) 69, 218
HphI GGTGA 2 cut(s) 233, 1387
Hpy166II GTNNAC 1 cut(s) 1106
Hpy188I TCNGA 3 cut(s) 1147, 1188, 1257
Hpy188III TCNNGA 5 cut(s) 27, 88, 101, 203, 1174
Hpy8I GTNNAC 1 cut(s) 1106
Hpy99I CGWCG 1 cut(s) 1110
HpyAV CCTTC 5 cut(s) 86, 209, 961, 1088, 1430
HpyCH4III ACNGT 5 cut(s) 24, 306, 412, 528, 1437
HpyCH4IV ACGT 2 cut(s) 1075, 1108
HpyF10VI GCNNNNNNNGC 1 cut(s) 566
HpyF3I CTNAG 5 cut(s) 63, 450, 489, 588, 1385
HpySE526I ACGT 2 cut(s) 1075, 1108
Hsp92I GRCGYC 1 cut(s) 10
HspAI GCGC 1 cut(s) 10
KasI GGCGCC 1 cut(s) 9
Kzo9I GATC 5 cut(s) 16, 29, 1008, 1161, 1252
LguI GCTCTTC 1 cut(s) 534
Lsp1109I GCAGC 3 cut(s) 81, 286, 287
LweI GCATC 4 cut(s) 278, 286, 663, 1167
MaeI CTAG 5 cut(s) 425, 894, 1154, 1332, 1400
MaeII ACGT 2 cut(s) 1075, 1108
MaeIII GTNAC 7 cut(s) 170, 832, 875, 1111, 1133, 1218, 1431
MalI GATC 5 cut(s) 18, 31, 1010, 1163, 1254
MboI GATC 5 cut(s) 16, 29, 1008, 1161, 1252
MboII GAAGA 9 cut(s) 107, 410, 551, 804, 913, 1016, 1160, 1218, 1275
MfeI CAATTG 1 cut(s) 561
MflI RGATCY 3 cut(s) 29, 1161, 1252
MhlI GDGCHC 1 cut(s) 642
MluCI AATT 7 cut(s) 194, 561, 783, 813, 866, 1080, 1126
Mly113I GGCGCC 1 cut(s) 10
MlyI GAGTC 1 cut(s) 463
MmeI TCCRAC 1 cut(s) 213
Mph1103I ATGCAT 1 cut(s) 1182
MseI TTAA 2 cut(s) 192, 906
MspA1I CMGCKG 1 cut(s) 395
MspI CCGG 2 cut(s) 69, 218
MspR9I CCNGG 3 cut(s) 37, 219, 756
MunI CAATTG 1 cut(s) 561
Mva1269I GAATGC 1 cut(s) 1333
MvaI CCWGG 2 cut(s) 37, 756
MwoI GCNNNNNNNGC 1 cut(s) 566
NarI GGCGCC 1 cut(s) 10
NciI CCSGG 1 cut(s) 219
NcoI CCATGG 2 cut(s) 319, 771
NdeI CATATG 2 cut(s) 735, 804
NdeII GATC 5 cut(s) 16, 29, 1008, 1161, 1252
NlaIV GGNNCC 3 cut(s) 11, 886, 1163
NmuCI GTSAC 4 cut(s) 875, 1111, 1133, 1431
NsiI ATGCAT 1 cut(s) 1182
NspI RCATGY 2 cut(s) 250, 573
NspV TTCGAA 1 cut(s) 1261
PaeI GCATGC 1 cut(s) 573
PceI AGGCCT 1 cut(s) 1419
PciI ACATGT 1 cut(s) 246
PciSI GCTCTTC 1 cut(s) 534
PctI GAATGC 1 cut(s) 1333
PfeI GAWTC 2 cut(s) 924, 1258
PfoI TCCNGGA 1 cut(s) 754
PkrI GCNGC 3 cut(s) 96, 277, 301
PleI GAGTC 1 cut(s) 463
PluTI GGCGCC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 463
PscI ACATGT 1 cut(s) 246
Psp124BI GAGCTC 1 cut(s) 642
Psp6I CCWGG 2 cut(s) 35, 754
PspGI CCWGG 2 cut(s) 35, 754
PspN4I GGNNCC 3 cut(s) 11, 886, 1163
PspPI GGNCC 6 cut(s) 39, 215, 493, 697, 849, 884
PstNI CAGNNNCTG 1 cut(s) 94
PsuI RGATCY 3 cut(s) 29, 1161, 1252
PvuII CAGCTG 1 cut(s) 395
RsaI GTAC 5 cut(s) 251, 383, 431, 648, 843
RsaNI GTAC 5 cut(s) 250, 382, 430, 647, 842
SacI GAGCTC 1 cut(s) 642
SalI GTCGAC 1 cut(s) 1104
SapI GCTCTTC 1 cut(s) 534
SaqAI TTAA 2 cut(s) 192, 906
SatI GCNGC 3 cut(s) 95, 276, 300
Sau3AI GATC 5 cut(s) 16, 29, 1008, 1161, 1252
Sau96I GGNCC 6 cut(s) 39, 215, 493, 697, 849, 884
SchI GAGTC 1 cut(s) 463
ScrFI CCNGG 3 cut(s) 37, 219, 756
SduI GDGCHC 1 cut(s) 642
SfaNI GCATC 4 cut(s) 278, 286, 663, 1167
SfoI GGCGCC 1 cut(s) 11
SfuI TTCGAA 1 cut(s) 1261
SinI GGWCC 2 cut(s) 849, 884
SmlI CTYRAG 1 cut(s) 641
SmoI CTYRAG 1 cut(s) 641
SphI GCATGC 1 cut(s) 573
Sse9I AATT 7 cut(s) 194, 561, 783, 813, 866, 1080, 1126
SseBI AGGCCT 1 cut(s) 1419
SsiI CCGC 3 cut(s) 33, 514, 728
SspDI GGCGCC 1 cut(s) 9
SspI AATATT 1 cut(s) 622
SspMI CTAG 5 cut(s) 425, 894, 1154, 1332, 1400
SstI GAGCTC 1 cut(s) 642
StuI AGGCCT 1 cut(s) 1419
StyD4I CCNGG 3 cut(s) 35, 217, 754
StyI CCWWGG 4 cut(s) 319, 339, 700, 771
TaaI ACNGT 5 cut(s) 24, 306, 412, 528, 1437
TaiI ACGT 2 cut(s) 1078, 1111
TaqI TCGA 7 cut(s) 26, 333, 781, 949, 1105, 1261, 1356
TaqII GACCGA 1 cut(s) 866
TasI AATT 7 cut(s) 194, 561, 783, 813, 866, 1080, 1126
TatI WGTACW 2 cut(s) 429, 841
TfiI GAWTC 2 cut(s) 924, 1258
Tru1I TTAA 2 cut(s) 192, 906
Tru9I TTAA 2 cut(s) 192, 906
TscAI CASTG 5 cut(s) 451, 533, 580, 886, 1289
TseFI GTSAC 4 cut(s) 875, 1111, 1133, 1431
TseI GCWGC 3 cut(s) 94, 275, 299
Tsp45I GTSAC 4 cut(s) 875, 1111, 1133, 1431
TspDTI ATGAA 8 cut(s) 146, 519, 552, 612, 954, 1085, 1107, 1310
TspGWI ACGGA 2 cut(s) 267, 670
TspRI CASTG 5 cut(s) 451, 533, 580, 886, 1289
VpaK11BI GGWCC 2 cut(s) 849, 884
XapI RAATTY 4 cut(s) 194, 783, 813, 866
XceI RCATGY 2 cut(s) 250, 573
XmiI GTMKAC 1 cut(s) 1105
XspI CTAG 5 cut(s) 425, 894, 1154, 1332, 1400
Zsp2I ATGCAT 1 cut(s) 1182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.