AT3G25870

protein kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007828
Physical Location & Seq
Reverse (-)
9463305 .. 9464676
1372 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G25870.1

Sequence Viewer

Length: 513 bp
ATGGAGGATTTATTGGAGGAGAGGCTAACTCGGACTGACTCTGTCGGGAATAAACGAGTCAGAGACGGGTTAGACCTCGACTCGCCGGACGTGAAGCGGTTAAGAGATGATTTGTTCGATGACTCGGGGCTTGACCCAGTGAGTCAAGATCTTGACTCGGTGATGAAGAGTTTCGAGAATGAGTTATCGACAACGACGGCGGCGCTAAGCTCCGGTGAAACTCAGCCGGATCTTGGATACCTTTTCGAAGCTTCTGATGACGAGCTGGGTTTGCCACCGCCGCTTACGCCACCGCAAACGCTTCTTCCTCCGTCGTGTGAGGAAACGGTGACGGAATTGGTACGAGCCTCGTCGGATTCATCAGAGGTTGGAGAGTTATGTGGGTTTGAGGATCATGTTACGGAGTTCGGACCTTGCGATCTTGGTGATGATGGGCTTTTCGAATATTTCGATGGGTGTTTAGATTCCGGCGATTTGTTTTCATGGCGGCCGGAGTTTTTACCGGCGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

170

Amino Acids

18.67

Weight (kDa)

4.05

Isoelectric Point (pI)

56.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 7 cut(s) 97, 200, 278, 281, 293, 487, 506
AclWI GGATC 2 cut(s) 237, 399
AcoI YGGCCR 1 cut(s) 488
AfaI GTAC 1 cut(s) 342
AfiI CCNNNNNNNGG 1 cut(s) 233
AjiI CACGTC 1 cut(s) 91
AluBI AGCT 3 cut(s) 210, 251, 265
AluI AGCT 3 cut(s) 210, 251, 265
Alw26I GTCTC 1 cut(s) 57
AlwI GGATC 2 cut(s) 237, 399
Ama87I CYCGRG 1 cut(s) 124
AoxI GGCC 1 cut(s) 488
Asp700I GAANNNNTTC 1 cut(s) 170
AspLEI GCGC 1 cut(s) 205
AspS9I GGNCC 1 cut(s) 410
AsuHPI GGTGA 4 cut(s) 172, 227, 340, 437
AsuII TTCGAA 2 cut(s) 246, 441
AvaI CYCGRG 1 cut(s) 124
AvaII GGWCC 1 cut(s) 410
BccI CCATC 2 cut(s) 425, 446
BceAI ACGGC 1 cut(s) 213
BciVI GTATCC 1 cut(s) 230
BcoDI GTCTC 1 cut(s) 57
BfoI RGCGCY 1 cut(s) 206
BfuI GTATCC 1 cut(s) 230
BglII AGATCT 1 cut(s) 148
BisI GCNGC 3 cut(s) 201, 281, 488
BlpI GCTNAGC 1 cut(s) 206
BlsI GCNGC 3 cut(s) 202, 282, 489
Bme18I GGWCC 1 cut(s) 410
BmeT110I CYCGRG 1 cut(s) 124
BmgBI CACGTC 1 cut(s) 91
BmgT120I GGNCC 1 cut(s) 410
BmrI ACTGGG 1 cut(s) 131
BmuI ACTGGG 1 cut(s) 131
BplI GAGNNNNNCTC 2 cut(s) 13, 45
Bpu1102I GCTNAGC 1 cut(s) 206
Bpu14I TTCGAA 2 cut(s) 246, 441
BsaWI WCCGGW 1 cut(s) 212
Bsc4I CCNNNNNNNGG 1 cut(s) 233
Bse118I RCCGGY 1 cut(s) 502
Bse1I ACTGG 1 cut(s) 137
BseLI CCNNNNNNNGG 1 cut(s) 233
BseMII CTCAG 1 cut(s) 236
BseNI ACTGG 1 cut(s) 137
BseRI GAGGAG 1 cut(s) 32
BseX3I CGGCCG 1 cut(s) 488
BseYI CCCAGC 1 cut(s) 265
Bsh1285I CGRYCG 1 cut(s) 491
BshFI GGCC 1 cut(s) 490
BsiEI CGRYCG 1 cut(s) 491
BsiHKCI CYCGRG 1 cut(s) 124
BsiSI CCGG 6 cut(s) 86, 213, 227, 468, 491, 503
BslI CCNNNNNNNGG 1 cut(s) 233
BsmAI GTCTC 1 cut(s) 57
BsmBI CGTCTC 1 cut(s) 57
BsnI GGCC 1 cut(s) 490
BsoBI CYCGRG 1 cut(s) 124
Bsp119I TTCGAA 2 cut(s) 246, 441
Bsp143I GATC 4 cut(s) 148, 229, 391, 418
Bsp1720I GCTNAGC 1 cut(s) 206
BspACI CCGC 7 cut(s) 97, 200, 278, 281, 293, 487, 506
BspANI GGCC 1 cut(s) 490
BspCNI CTCAG 1 cut(s) 235
BspPI GGATC 2 cut(s) 237, 399
BspT104I TTCGAA 2 cut(s) 246, 441
BsrFI RCCGGY 1 cut(s) 502
BsrI ACTGG 1 cut(s) 137
BssAI RCCGGY 1 cut(s) 502
BssMI GATC 4 cut(s) 148, 229, 391, 418
Bst4CI ACNGT 1 cut(s) 328
Bst6I CTCTTC 1 cut(s) 161
BstBI TTCGAA 2 cut(s) 246, 441
BstDEI CTNAG 2 cut(s) 206, 222
BstH2I RGCGCY 1 cut(s) 206
BstHHI GCGC 1 cut(s) 205
BstKTI GATC 4 cut(s) 151, 232, 394, 421
BstMAI GTCTC 1 cut(s) 57
BstMBI GATC 4 cut(s) 148, 229, 391, 418
BstMCI CGRYCG 1 cut(s) 491
BstMWI GCNNNNNNNGC 3 cut(s) 271, 280, 286
BstX2I RGATCY 2 cut(s) 148, 229
BstYI RGATCY 2 cut(s) 148, 229
BstZI CGGCCG 1 cut(s) 488
BsuI GTATCC 1 cut(s) 230
BsuRI GGCC 1 cut(s) 490
BtrI CACGTC 1 cut(s) 91
BtsIMutI CAGTG 1 cut(s) 144
CfoI GCGC 1 cut(s) 205
Cfr10I RCCGGY 1 cut(s) 502
Cfr13I GGNCC 1 cut(s) 410
Csp6I GTAC 1 cut(s) 341
CviAII CATG 2 cut(s) 395, 483
CviJI RGCY 9 cut(s) 25, 130, 210, 226, 251, 265, 347, 436, 490
CviKI_1 RGCY 9 cut(s) 25, 130, 210, 226, 251, 265, 347, 436, 490
CviQI GTAC 1 cut(s) 341
DdeI CTNAG 2 cut(s) 206, 222
DpnI GATC 4 cut(s) 150, 231, 393, 420
DpnII GATC 4 cut(s) 148, 229, 391, 418
EaeI YGGCCR 1 cut(s) 488
EagI CGGCCG 1 cut(s) 488
Eam1104I CTCTTC 1 cut(s) 161
EarI CTCTTC 1 cut(s) 161
EclXI CGGCCG 1 cut(s) 488
Eco47I GGWCC 1 cut(s) 410
Eco52I CGGCCG 1 cut(s) 488
Eco88I CYCGRG 1 cut(s) 124
Esp3I CGTCTC 1 cut(s) 57
FaeI CATG 2 cut(s) 398, 486
FaiI YATR 3 cut(s) 379, 396, 484
FatI CATG 2 cut(s) 394, 482
Fnu4HI GCNGC 3 cut(s) 201, 281, 488
Fsp4HI GCNGC 3 cut(s) 201, 281, 488
GlaI GCGC 1 cut(s) 204
GluI GCNGC 3 cut(s) 201, 281, 488
GsaI CCCAGC 1 cut(s) 269
HaeII RGCGCY 1 cut(s) 206
HaeIII GGCC 1 cut(s) 490
HapII CCGG 6 cut(s) 86, 213, 227, 468, 491, 503
HhaI GCGC 1 cut(s) 205
Hin1II CATG 2 cut(s) 398, 486
Hin6I GCGC 1 cut(s) 203
HinP1I GCGC 1 cut(s) 203
HindIII AAGCTT 1 cut(s) 249
HinfI GANTC 8 cut(s) 38, 57, 80, 122, 142, 155, 356, 464
HpaII CCGG 6 cut(s) 86, 213, 227, 468, 491, 503
HphI GGTGA 4 cut(s) 172, 227, 340, 437
Hpy188I TCNGA 6 cut(s) 33, 62, 256, 355, 364, 410
Hpy188III TCNNGA 4 cut(s) 46, 146, 152, 175
Hpy99I CGWCG 3 cut(s) 199, 316, 355
HpyCH4III ACNGT 1 cut(s) 328
HpyCH4IV ACGT 1 cut(s) 90
HpyF10VI GCNNNNNNNGC 3 cut(s) 271, 280, 286
HpyF3I CTNAG 2 cut(s) 206, 222
HpySE526I ACGT 1 cut(s) 90
Hsp92II CATG 2 cut(s) 398, 486
HspAI GCGC 1 cut(s) 203
Kzo9I GATC 4 cut(s) 148, 229, 391, 418
LmnI GCTCC 1 cut(s) 215
LpnPI CCDG 7 cut(s) 99, 150, 226, 240, 251, 481, 504
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 2 cut(s) 328, 397
MalI GATC 4 cut(s) 150, 231, 393, 420
MboI GATC 4 cut(s) 148, 229, 391, 418
MboII GAAGA 2 cut(s) 178, 296
MflI RGATCY 2 cut(s) 148, 229
MluCI AATT 1 cut(s) 335
MlyI GAGTC 6 cut(s) 32, 66, 74, 116, 149, 151
MmeI TCCRAC 2 cut(s) 333, 349
MnlI CCTC 8 cut(s) 10, 15, 86, 313, 318, 358, 358, 382
MroXI GAANNNNTTC 1 cut(s) 170
MseI TTAA 1 cut(s) 101
MspI CCGG 6 cut(s) 86, 213, 227, 468, 491, 503
MwoI GCNNNNNNNGC 3 cut(s) 271, 280, 286
NdeII GATC 4 cut(s) 148, 229, 391, 418
NlaIII CATG 2 cut(s) 398, 486
NmuCI GTSAC 1 cut(s) 328
NspV TTCGAA 2 cut(s) 246, 441
PcsI WCGNNNNNNNCGW 2 cut(s) 414, 447
PdmI GAANNNNTTC 1 cut(s) 170
PfeI GAWTC 2 cut(s) 356, 464
PflFI GACNNNGTC 1 cut(s) 41
PkrI GCNGC 3 cut(s) 202, 282, 489
PleI GAGTC 6 cut(s) 32, 65, 74, 116, 149, 150
PpsI GAGTC 6 cut(s) 32, 65, 74, 116, 149, 150
PspFI CCCAGC 1 cut(s) 265
PspPI GGNCC 1 cut(s) 410
PsuI RGATCY 2 cut(s) 148, 229
PsyI GACNNNGTC 1 cut(s) 41
RsaI GTAC 1 cut(s) 342
RsaNI GTAC 1 cut(s) 341
SaqAI TTAA 1 cut(s) 101
SatI GCNGC 3 cut(s) 201, 281, 488
Sau3AI GATC 4 cut(s) 148, 229, 391, 418
Sau96I GGNCC 1 cut(s) 410
SchI GAGTC 6 cut(s) 32, 66, 74, 116, 149, 151
SetI ASST 8 cut(s) 78, 93, 212, 243, 253, 267, 369, 415
SfuI TTCGAA 2 cut(s) 246, 441
SinI GGWCC 1 cut(s) 410
Sse9I AATT 1 cut(s) 335
SsiI CCGC 7 cut(s) 97, 200, 278, 281, 293, 487, 506
SspI AATATT 1 cut(s) 446
TaaI ACNGT 1 cut(s) 328
TaiI ACGT 1 cut(s) 93
TaqI TCGA 7 cut(s) 78, 117, 174, 188, 246, 441, 450
TasI AATT 1 cut(s) 335
TauI GCSGC 3 cut(s) 203, 283, 490
TfiI GAWTC 2 cut(s) 356, 464
Tru1I TTAA 1 cut(s) 101
Tru9I TTAA 1 cut(s) 101
TscAI CASTG 1 cut(s) 144
TseFI GTSAC 1 cut(s) 328
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 3 cut(s) 179, 348, 471
TspGWI ACGGA 3 cut(s) 300, 347, 416
TspRI CASTG 1 cut(s) 144
Tth111I GACNNNGTC 1 cut(s) 41
VpaK11BI GGWCC 1 cut(s) 410
XmnI GAANNNNTTC 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.