pycom13g03770

protein kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0007828
Physical Location & Seq
Forward (+)
2506219 .. 2506767
549 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g03770.1

Sequence Viewer

Length: 549 bp
ATGGAGGACCACACCCCAACTAACAAGAAGCGGGTCCGAGACAACTCGGACGACTTGATTCACGACTCGGCCGAGGTGAAGCGACTCAGGGAAGACCTACTGGGTTTCCTTGACGACTCGGACGCCGATCCCACGACTCAGGACCTCGACTCGGTCATGAAAAGCTTCGAGGAAGAGATAGCGGCCGCAACCACTTCTTGCCGGTCGTCGCCGCGTCCGGCATCGGAGACTGAGCCGGCTCTGGTGCCGGTGGTTGACTTGACGTCGGAATCTGCCGAGTCCAATCCCAACCTCGGGTACCTTCTAGAAGCTTCGGACGACGAGCTGGGCCTGCCCCCGTCGGAGAACTTCAGCGAGGAGGCGGCGAAAGACGGGGAGAGCGAGTTGGTCCGAGTTCCGTCCGACACGTCCGGAGCCGACGAGTTTTGGCAGTTCGACGATCCCACTCCGAGTACGTTTGACGGGCTGTTCGGAAACGACGATGTGTATTTTGACTCGGCTGATTACGGTGAATTTTCGTGGCGTTTCGAAACACTGCCAGCGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

183

Amino Acids

20.1

Weight (kDa)

4.05

Isoelectric Point (pI)

55.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 266
Acc65I GGTACC 1 cut(s) 297
AccB1I GGYRCC 2 cut(s) 244, 297
AccII CGCG 1 cut(s) 214
AccIII TCCGGA 1 cut(s) 410
AciI CCGC 5 cut(s) 31, 182, 186, 212, 362
AclWI GGATC 2 cut(s) 122, 434
AcoI YGGCCR 2 cut(s) 69, 183
AcsI RAATTY 1 cut(s) 512
AcuI CTGAAG 1 cut(s) 334
AcyI GRCGYC 2 cut(s) 123, 263
AfaI GTAC 2 cut(s) 299, 454
AfiI CCNNNNNNNGG 3 cut(s) 151, 293, 294
AflIII ACRYGT 1 cut(s) 405
AhdI GACNNNNNGTC 1 cut(s) 262
AjiI CACGTC 1 cut(s) 408
AluBI AGCT 3 cut(s) 165, 311, 325
AluI AGCT 3 cut(s) 165, 311, 325
Alw26I GTCTC 2 cut(s) 33, 221
AlwI GGATC 2 cut(s) 122, 434
Ama87I CYCGRG 1 cut(s) 293
Aor13HI TCCGGA 1 cut(s) 410
AoxI GGCC 3 cut(s) 69, 183, 328
ApoI RAATTY 1 cut(s) 512
ArsI GACNNNNNNTTYG 2 cut(s) 452, 484
Asp700I GAANNNNTTC 1 cut(s) 164
Asp718I GGTACC 1 cut(s) 297
AspLEI GCGC 1 cut(s) 544
AspS9I GGNCC 5 cut(s) 7, 34, 142, 328, 388
AsuHPI GGTGA 2 cut(s) 88, 521
AsuII TTCGAA 1 cut(s) 528
AvaI CYCGRG 1 cut(s) 293
AvaII GGWCC 4 cut(s) 7, 34, 142, 388
BanI GGYRCC 2 cut(s) 244, 297
BbsI GAAGAC 1 cut(s) 99
BcoDI GTCTC 2 cut(s) 33, 221
BfaI CTAG 1 cut(s) 305
BisI GCNGC 4 cut(s) 183, 186, 212, 363
BlsI GCNGC 4 cut(s) 184, 187, 213, 364
Bme18I GGWCC 4 cut(s) 7, 34, 142, 388
BmeRI GACNNNNNGTC 1 cut(s) 262
BmeT110I CYCGRG 1 cut(s) 293
BmgBI CACGTC 1 cut(s) 408
BmgT120I GGNCC 5 cut(s) 7, 34, 142, 328, 388
BmiI GGNNCC 4 cut(s) 35, 246, 299, 415
BmrI ACTGGG 1 cut(s) 110
BmsI GCATC 1 cut(s) 230
BmuI ACTGGG 1 cut(s) 110
BpiI GAAGAC 1 cut(s) 99
Bpu14I TTCGAA 1 cut(s) 528
BsaHI GRCGYC 2 cut(s) 123, 263
BsaJI CCNNGG 2 cut(s) 72, 292
BsaWI WCCGGW 1 cut(s) 410
BsaXI ACNNNNNCTCC 2 cut(s) 368, 398
Bsc4I CCNNNNNNNGG 3 cut(s) 151, 293, 294
Bse118I RCCGGY 3 cut(s) 201, 235, 247
Bse1I ACTGG 1 cut(s) 105
BseAI TCCGGA 1 cut(s) 410
BseDI CCNNGG 2 cut(s) 72, 292
BseLI CCNNNNNNNGG 3 cut(s) 151, 293, 294
BseMII CTCAG 3 cut(s) 100, 152, 222
BseNI ACTGG 1 cut(s) 105
BseRI GAGGAG 1 cut(s) 371
BseX3I CGGCCG 2 cut(s) 69, 183
BseYI CCCAGC 1 cut(s) 325
Bsh1236I CGCG 1 cut(s) 214
Bsh1285I CGRYCG 3 cut(s) 72, 186, 206
BshFI GGCC 3 cut(s) 71, 185, 330
BshNI GGYRCC 2 cut(s) 244, 297
BsiEI CGRYCG 3 cut(s) 72, 186, 206
BsiHKCI CYCGRG 1 cut(s) 293
BsiSI CCGG 5 cut(s) 202, 218, 236, 248, 411
BslI CCNNNNNNNGG 3 cut(s) 151, 293, 294
BsmAI GTCTC 2 cut(s) 33, 221
BsnI GGCC 3 cut(s) 71, 185, 330
BsoBI CYCGRG 1 cut(s) 293
Bsp119I TTCGAA 1 cut(s) 528
Bsp13I TCCGGA 1 cut(s) 410
Bsp143I GATC 2 cut(s) 127, 439
BspACI CCGC 5 cut(s) 31, 182, 186, 212, 362
BspANI GGCC 3 cut(s) 71, 185, 330
BspCNI CTCAG 3 cut(s) 99, 151, 223
BspEI TCCGGA 1 cut(s) 410
BspFNI CGCG 1 cut(s) 214
BspHI TCATGA 1 cut(s) 156
BspLI GGNNCC 4 cut(s) 35, 246, 299, 415
BspPI GGATC 2 cut(s) 122, 434
BspT104I TTCGAA 1 cut(s) 528
BspT107I GGYRCC 2 cut(s) 244, 297
BsrFI RCCGGY 3 cut(s) 201, 235, 247
BsrI ACTGG 1 cut(s) 105
BssAI RCCGGY 3 cut(s) 201, 235, 247
BssECI CCNNGG 2 cut(s) 72, 292
BssMI GATC 2 cut(s) 127, 439
BssNI GRCGYC 2 cut(s) 123, 263
Bst4CI ACNGT 1 cut(s) 509
Bst6I CTCTTC 1 cut(s) 168
BstACI GRCGYC 2 cut(s) 123, 263
BstBI TTCGAA 1 cut(s) 528
BstC8I GCNNGC 3 cut(s) 237, 332, 540
BstDEI CTNAG 3 cut(s) 86, 138, 231
BstFNI CGCG 1 cut(s) 214
BstHHI GCGC 1 cut(s) 544
BstKTI GATC 2 cut(s) 130, 442
BstMAI GTCTC 2 cut(s) 33, 221
BstMBI GATC 2 cut(s) 127, 439
BstMCI CGRYCG 3 cut(s) 72, 186, 206
BstMWI GCNNNNNNNGC 1 cut(s) 331
BstUI CGCG 1 cut(s) 214
BstV2I GAAGAC 1 cut(s) 99
BstZI CGGCCG 2 cut(s) 69, 183
BsuRI GGCC 3 cut(s) 71, 185, 330
BtrI CACGTC 1 cut(s) 408
BtsI GCAGTG 1 cut(s) 533
BtsIMutI CAGTG 1 cut(s) 533
Cac8I GCNNGC 3 cut(s) 237, 332, 540
CciI TCATGA 1 cut(s) 156
CciNI GCGGCCGC 1 cut(s) 183
CfoI GCGC 1 cut(s) 544
Cfr10I RCCGGY 3 cut(s) 201, 235, 247
Cfr13I GGNCC 5 cut(s) 7, 34, 142, 328, 388
CseI GACGC 2 cut(s) 131, 203
Csp6I GTAC 2 cut(s) 298, 453
CviAII CATG 1 cut(s) 157
CviQI GTAC 2 cut(s) 298, 453
DdeI CTNAG 3 cut(s) 86, 138, 231
DpnI GATC 2 cut(s) 129, 441
DpnII GATC 2 cut(s) 127, 439
DriI GACNNNNNGTC 1 cut(s) 262
EaeI YGGCCR 2 cut(s) 69, 183
EagI CGGCCG 2 cut(s) 69, 183
Eam1104I CTCTTC 1 cut(s) 168
Eam1105I GACNNNNNGTC 1 cut(s) 262
EarI CTCTTC 1 cut(s) 168
EclXI CGGCCG 2 cut(s) 69, 183
Eco47I GGWCC 4 cut(s) 7, 34, 142, 388
Eco52I CGGCCG 2 cut(s) 69, 183
Eco57I CTGAAG 1 cut(s) 334
Eco88I CYCGRG 1 cut(s) 293
EcoO109I RGGNCCY 1 cut(s) 142
FaeI CATG 1 cut(s) 160
FaiI YATR 1 cut(s) 158
FatI CATG 1 cut(s) 156
FauI CCCGC 1 cut(s) 24
Fnu4HI GCNGC 4 cut(s) 183, 186, 212, 363
Fsp4HI GCNGC 4 cut(s) 183, 186, 212, 363
FspBI CTAG 1 cut(s) 305
GlaI GCGC 1 cut(s) 543
GluI GCNGC 4 cut(s) 183, 186, 212, 363
GsaI CCCAGC 1 cut(s) 329
HaeIII GGCC 3 cut(s) 71, 185, 330
HapII CCGG 5 cut(s) 202, 218, 236, 248, 411
HgaI GACGC 2 cut(s) 131, 203
HhaI GCGC 1 cut(s) 544
Hin1I GRCGYC 2 cut(s) 123, 263
Hin1II CATG 1 cut(s) 160
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HindIII AAGCTT 2 cut(s) 163, 309
HinfI GANTC 9 cut(s) 58, 65, 84, 116, 136, 149, 269, 278, 494
HpaII CCGG 5 cut(s) 202, 218, 236, 248, 411
HphI GGTGA 2 cut(s) 88, 521
Hpy166II GTNNAC 1 cut(s) 256
Hpy188III TCNNGA 5 cut(s) 62, 140, 157, 305, 411
Hpy8I GTNNAC 1 cut(s) 256
Hpy99I CGWCG 7 cut(s) 211, 268, 323, 343, 422, 440, 482
HpyAV CCTTC 1 cut(s) 311
HpyCH4III ACNGT 1 cut(s) 509
HpyCH4IV ACGT 3 cut(s) 263, 407, 455
HpyF10VI GCNNNNNNNGC 1 cut(s) 331
HpyF3I CTNAG 3 cut(s) 86, 138, 231
HpySE526I ACGT 3 cut(s) 263, 407, 455
Hsp92I GRCGYC 2 cut(s) 123, 263
Hsp92II CATG 1 cut(s) 160
HspAI GCGC 1 cut(s) 542
Kpn2I TCCGGA 1 cut(s) 410
KpnI GGTACC 1 cut(s) 301
KroI GCCGGC 1 cut(s) 235
KroNI GCCGGC 1 cut(s) 237
Kzo9I GATC 2 cut(s) 127, 439
LmnI GCTCC 1 cut(s) 413
LweI GCATC 1 cut(s) 230
MaeI CTAG 1 cut(s) 305
MaeII ACGT 3 cut(s) 263, 407, 455
MalI GATC 2 cut(s) 129, 441
MboI GATC 2 cut(s) 127, 439
MboII GAAGA 2 cut(s) 104, 185
MluCI AATT 1 cut(s) 512
MlyI GAGTC 7 cut(s) 59, 78, 110, 130, 143, 287, 488
MmeI TCCRAC 3 cut(s) 246, 321, 426
MnlI CCTC 6 cut(s) 67, 155, 163, 302, 349, 352
MroI TCCGGA 1 cut(s) 410
MroNI GCCGGC 1 cut(s) 235
MroXI GAANNNNTTC 1 cut(s) 164
MspI CCGG 5 cut(s) 202, 218, 236, 248, 411
MvnI CGCG 1 cut(s) 214
MwoI GCNNNNNNNGC 1 cut(s) 331
NaeI GCCGGC 1 cut(s) 237
NdeII GATC 2 cut(s) 127, 439
NgoMIV GCCGGC 1 cut(s) 235
NlaIII CATG 1 cut(s) 160
NlaIV GGNNCC 4 cut(s) 35, 246, 299, 415
NmeAIII GCCGAG 4 cut(s) 47, 97, 301, 476
NotI GCGGCCGC 1 cut(s) 183
NspV TTCGAA 1 cut(s) 528
PagI TCATGA 1 cut(s) 156
PcsI WCGNNNNNNNCGW 3 cut(s) 69, 378, 477
PdiI GCCGGC 1 cut(s) 237
PdmI GAANNNNTTC 1 cut(s) 164
PfeI GAWTC 2 cut(s) 58, 269
PflFI GACNNNGTC 1 cut(s) 152
PkrI GCNGC 4 cut(s) 184, 187, 213, 364
PleI GAGTC 7 cut(s) 59, 78, 110, 130, 143, 286, 488
PpsI GAGTC 7 cut(s) 59, 78, 110, 130, 143, 286, 488
PpuMI RGGWCCY 1 cut(s) 142
Psp5II RGGWCCY 1 cut(s) 142
PspFI CCCAGC 1 cut(s) 325
PspN4I GGNNCC 4 cut(s) 35, 246, 299, 415
PspPI GGNCC 5 cut(s) 7, 34, 142, 328, 388
PspPPI RGGWCCY 1 cut(s) 142
PsyI GACNNNGTC 1 cut(s) 152
RsaI GTAC 2 cut(s) 299, 454
RsaNI GTAC 2 cut(s) 298, 453
SatI GCNGC 4 cut(s) 183, 186, 212, 363
Sau3AI GATC 2 cut(s) 127, 439
Sau96I GGNCC 5 cut(s) 7, 34, 142, 328, 388
SchI GAGTC 7 cut(s) 59, 78, 110, 130, 143, 287, 488
SfaNI GCATC 1 cut(s) 230
SfuI TTCGAA 1 cut(s) 528
SinI GGWCC 4 cut(s) 7, 34, 142, 388
Sse9I AATT 1 cut(s) 512
SsiI CCGC 5 cut(s) 31, 182, 186, 212, 362
SspMI CTAG 1 cut(s) 305
TaaI ACNGT 1 cut(s) 509
TaiI ACGT 3 cut(s) 266, 410, 458
TaqI TCGA 4 cut(s) 147, 168, 435, 528
TaqII GACCGA 1 cut(s) 142
TasI AATT 1 cut(s) 512
TauI GCSGC 4 cut(s) 185, 188, 214, 365
TfiI GAWTC 2 cut(s) 58, 269
TscAI CASTG 1 cut(s) 540
TspDTI ATGAA 1 cut(s) 173
TspGWI ACGGA 1 cut(s) 387
TspRI CASTG 1 cut(s) 540
Tth111I GACNNNGTC 1 cut(s) 152
VpaK11BI GGWCC 4 cut(s) 7, 34, 142, 388
XapI RAATTY 1 cut(s) 512
XbaI TCTAGA 1 cut(s) 304
XmnI GAANNNNTTC 1 cut(s) 164
XspI CTAG 1 cut(s) 305
ZraI GACGTC 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.