FvH4_4g27540

protein kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0007828
Physical Location & Seq
Forward (+)
28499286 .. 28500460
1175 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27540.t1

Sequence Viewer

Length: 588 bp
ATGGAAGACACGAATATCAAGAAGCGAGTTCGGGACTACGAGTCCGACTCGGTCGAAGCGAAGCGGCTACGAGAAGACCTACTGGAGGACTTCCTTGACGATTCCGATTCCGTTCCGGCGAGTCACGACCTCGACTCGTTCATGAAGAGCTTCGAGGAAGAGATCTCCGCTACTAATTCTTGCTCGACGTCGCCTCGTCCGGCGCCGGAATCTTCCGCCGCTCCGGTGGTGGTCGATCTGACGTCGGATTCCGGCGAGTCCAATCCGGATCTCACGCACCTTCTAGAAGCGTCGGATGACGAGCTCGGCCTTCCCCCGTCCGAAGGGTTCAGCGGCGAGGCGGCGAACGACAGGGAGTCTGAGTTGGTTAGAGTGTCGTCTGACTCGTCCGGGATCGGCGAGTTATGGCGGTTTGACGACCAGGGTCCGAGTTACGACTCGTTCGAGTTGAATGCCGGAGCTGGTAGTAACTTTGATGATAGCGGTTACGTGGCGTTTGATGGGCTGTTCGAGCACTCAGATGTGTATTTTGAATCGTCTGATTACTCCGATTTTTCGTGGCGCTTCGAAACGCCGCCTGCGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

21.44

Weight (kDa)

4.05

Isoelectric Point (pI)

54.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 191, 245
AccB1I GGYRCC 1 cut(s) 202
AccBSI CCGCTC 1 cut(s) 221
AccIII TCCGGA 1 cut(s) 265
AclWI GGATC 2 cut(s) 276, 401
AcyI GRCGYC 3 cut(s) 188, 203, 242
AfiI CCNNNNNNNGG 2 cut(s) 85, 323
AgsI TTSAA 2 cut(s) 451, 533
AhdI GACNNNNNGTC 2 cut(s) 40, 355
AjnI CCWGG 1 cut(s) 420
AluBI AGCT 3 cut(s) 150, 304, 461
AluI AGCT 3 cut(s) 150, 304, 461
Alw21I GWGCWC 2 cut(s) 306, 516
AlwI GGATC 2 cut(s) 276, 401
Aor13HI TCCGGA 1 cut(s) 265
AoxI GGCC 1 cut(s) 307
Asp700I GAANNNNTTC 1 cut(s) 149
AspLEI GCGC 2 cut(s) 205, 564
AspS9I GGNCC 1 cut(s) 425
AsuC2I CCSGG 1 cut(s) 391
AsuII TTCGAA 1 cut(s) 567
AvaII GGWCC 1 cut(s) 425
BanI GGYRCC 1 cut(s) 202
BanII GRGCYC 1 cut(s) 306
BbsI GAAGAC 2 cut(s) 12, 81
Bbv12I GWGCWC 2 cut(s) 306, 516
BccI CCATC 1 cut(s) 494
BcgI CGANNNNNNTGC 2 cut(s) 434, 468
BciT130I CCWGG 1 cut(s) 422
BcnI CCSGG 1 cut(s) 391
BfaI CTAG 1 cut(s) 284
BfoI RGCGCY 2 cut(s) 206, 565
BglII AGATCT 1 cut(s) 162
BisI GCNGC 5 cut(s) 65, 219, 334, 342, 575
BlsI GCNGC 5 cut(s) 66, 220, 335, 343, 576
Bme1390I CCNGG 2 cut(s) 391, 422
Bme18I GGWCC 1 cut(s) 425
BmeRI GACNNNNNGTC 2 cut(s) 40, 355
BmgT120I GGNCC 1 cut(s) 425
BmiI GGNNCC 2 cut(s) 204, 426
BmrFI CCNGG 2 cut(s) 391, 422
BoxI GACNNNNGTC 1 cut(s) 423
BpiI GAAGAC 2 cut(s) 12, 81
BplI GAGNNNNNCTC 2 cut(s) 32, 64
BpmI CTGGAG 1 cut(s) 104
Bpu14I TTCGAA 1 cut(s) 567
BpuMI CCSGG 1 cut(s) 391
BsaAI YACGTR 1 cut(s) 490
BsaHI GRCGYC 3 cut(s) 188, 203, 242
BsaJI CCNNGG 1 cut(s) 421
BsaWI WCCGGW 2 cut(s) 223, 265
BsaXI ACNNNNNCTCC 2 cut(s) 347, 377
Bsc4I CCNNNNNNNGG 2 cut(s) 85, 323
Bse1I ACTGG 1 cut(s) 87
BseAI TCCGGA 1 cut(s) 265
BseBI CCWGG 1 cut(s) 422
BseDI CCNNGG 1 cut(s) 421
BseGI GGATG 1 cut(s) 301
BseLI CCNNNNNNNGG 2 cut(s) 85, 323
BseMII CTCAG 2 cut(s) 351, 531
BseNI ACTGG 1 cut(s) 87
Bsh1285I CGRYCG 1 cut(s) 54
BshFI GGCC 1 cut(s) 309
BshNI GGYRCC 1 cut(s) 202
BsiEI CGRYCG 1 cut(s) 54
BsiHKAI GWGCWC 2 cut(s) 306, 516
BsiSI CCGG 8 cut(s) 116, 200, 206, 224, 252, 266, 390, 456
BslFI GGGAC 1 cut(s) 47
BslI CCNNNNNNNGG 2 cut(s) 85, 323
BsmFI GGGAC 1 cut(s) 47
BsmI GAATGC 1 cut(s) 457
BsnI GGCC 1 cut(s) 309
Bsp119I TTCGAA 1 cut(s) 567
Bsp1286I GDGCHC 2 cut(s) 306, 516
Bsp13I TCCGGA 1 cut(s) 265
Bsp143I GATC 4 cut(s) 162, 235, 268, 393
BspANI GGCC 1 cut(s) 309
BspCNI CTCAG 2 cut(s) 352, 530
BspEI TCCGGA 1 cut(s) 265
BspHI TCATGA 1 cut(s) 141
BspLI GGNNCC 2 cut(s) 204, 426
BspPI GGATC 2 cut(s) 276, 401
BspQI GCTCTTC 1 cut(s) 140
BspT104I TTCGAA 1 cut(s) 567
BspT107I GGYRCC 1 cut(s) 202
BsrBI CCGCTC 1 cut(s) 221
BsrI ACTGG 1 cut(s) 87
BssECI CCNNGG 1 cut(s) 421
BssMI GATC 4 cut(s) 162, 235, 268, 393
BssNI GRCGYC 3 cut(s) 188, 203, 242
Bst2UI CCWGG 1 cut(s) 422
Bst6I CTCTTC 2 cut(s) 140, 153
BstACI GRCGYC 3 cut(s) 188, 203, 242
BstBAI YACGTR 1 cut(s) 490
BstBI TTCGAA 1 cut(s) 567
BstC8I GCNNGC 1 cut(s) 579
BstDEI CTNAG 2 cut(s) 360, 517
BstENI CCTNNNNNAGG 1 cut(s) 83
BstF5I GGATG 1 cut(s) 301
BstH2I RGCGCY 2 cut(s) 206, 565
BstHHI GCGC 2 cut(s) 205, 564
BstKTI GATC 4 cut(s) 165, 238, 271, 396
BstMBI GATC 4 cut(s) 162, 235, 268, 393
BstMCI CGRYCG 1 cut(s) 54
BstMWI GCNNNNNNNGC 1 cut(s) 511
BstNI CCWGG 1 cut(s) 422
BstPAI GACNNNNGTC 1 cut(s) 423
BstSCI CCNGG 2 cut(s) 389, 420
BstV2I GAAGAC 2 cut(s) 12, 81
BstX2I RGATCY 2 cut(s) 162, 268
BstYI RGATCY 2 cut(s) 162, 268
BsuRI GGCC 1 cut(s) 309
BtsCI GGATG 1 cut(s) 301
Cac8I GCNNGC 1 cut(s) 579
CciI TCATGA 1 cut(s) 141
CfoI GCGC 2 cut(s) 205, 564
Cfr13I GGNCC 1 cut(s) 425
CseI GACGC 1 cut(s) 279
CviAII CATG 1 cut(s) 142
CviJI RGCY 6 cut(s) 67, 150, 304, 309, 461, 505
CviKI_1 RGCY 6 cut(s) 67, 150, 304, 309, 461, 505
DdeI CTNAG 2 cut(s) 360, 517
DinI GGCGCC 1 cut(s) 204
DpnI GATC 4 cut(s) 164, 237, 270, 395
DpnII GATC 4 cut(s) 162, 235, 268, 393
DriI GACNNNNNGTC 2 cut(s) 40, 355
Eam1104I CTCTTC 2 cut(s) 140, 153
Eam1105I GACNNNNNGTC 2 cut(s) 40, 355
EarI CTCTTC 2 cut(s) 140, 153
EciI GGCGGA 1 cut(s) 205
Ecl136II GAGCTC 1 cut(s) 304
Eco24I GRGCYC 1 cut(s) 306
Eco47I GGWCC 1 cut(s) 425
Eco53kI GAGCTC 1 cut(s) 304
EcoICRI GAGCTC 1 cut(s) 304
EcoNI CCTNNNNNAGG 1 cut(s) 83
EcoRII CCWGG 1 cut(s) 420
EcoT38I GRGCYC 1 cut(s) 306
EgeI GGCGCC 1 cut(s) 204
EheI GGCGCC 1 cut(s) 204
FaeI CATG 1 cut(s) 145
FaiI YATR 2 cut(s) 143, 406
FaqI GGGAC 1 cut(s) 47
FatI CATG 1 cut(s) 141
Fnu4HI GCNGC 5 cut(s) 65, 219, 334, 342, 575
FokI GGATG 1 cut(s) 308
FriOI GRGCYC 1 cut(s) 306
Fsp4HI GCNGC 5 cut(s) 65, 219, 334, 342, 575
FspBI CTAG 1 cut(s) 284
GlaI GCGC 2 cut(s) 204, 563
GluI GCNGC 5 cut(s) 65, 219, 334, 342, 575
GsuI CTGGAG 1 cut(s) 104
HaeII RGCGCY 2 cut(s) 206, 565
HaeIII GGCC 1 cut(s) 309
HapII CCGG 8 cut(s) 116, 200, 206, 224, 252, 266, 390, 456
HgaI GACGC 1 cut(s) 279
HhaI GCGC 2 cut(s) 205, 564
Hin1I GRCGYC 3 cut(s) 188, 203, 242
Hin1II CATG 1 cut(s) 145
Hin6I GCGC 2 cut(s) 203, 562
HinP1I GCGC 2 cut(s) 203, 562
HpaII CCGG 8 cut(s) 116, 200, 206, 224, 252, 266, 390, 456
Hpy188III TCNNGA 6 cut(s) 19, 32, 125, 142, 266, 284
Hpy99I CGWCG 4 cut(s) 190, 193, 247, 295
HpyAV CCTTC 3 cut(s) 290, 317, 320
HpyCH4IV ACGT 3 cut(s) 188, 242, 489
HpyF10VI GCNNNNNNNGC 1 cut(s) 511
HpyF3I CTNAG 2 cut(s) 360, 517
HpySE526I ACGT 3 cut(s) 188, 242, 489
Hsp92I GRCGYC 3 cut(s) 188, 203, 242
Hsp92II CATG 1 cut(s) 145
HspAI GCGC 2 cut(s) 203, 562
KasI GGCGCC 1 cut(s) 202
Kpn2I TCCGGA 1 cut(s) 265
Kzo9I GATC 4 cut(s) 162, 235, 268, 393
LguI GCTCTTC 1 cut(s) 140
LmnI GCTCC 2 cut(s) 226, 458
MaeI CTAG 1 cut(s) 284
MaeII ACGT 3 cut(s) 188, 242, 489
MaeIII GTNAC 4 cut(s) 122, 431, 467, 485
MalI GATC 4 cut(s) 164, 237, 270, 395
MbiI CCGCTC 1 cut(s) 221
MboI GATC 4 cut(s) 162, 235, 268, 393
MboII GAAGA 5 cut(s) 17, 86, 157, 170, 204
MflI RGATCY 2 cut(s) 162, 268
MhlI GDGCHC 2 cut(s) 306, 516
MluCI AATT 1 cut(s) 175
Mly113I GGCGCC 1 cut(s) 203
MlyI GAGTC 8 cut(s) 41, 50, 128, 130, 266, 365, 377, 431
MmeI TCCRAC 3 cut(s) 69, 225, 273
MnlI CCTC 5 cut(s) 79, 140, 148, 204, 331
MroI TCCGGA 1 cut(s) 265
MroXI GAANNNNTTC 1 cut(s) 149
MslI CAYNNNNRTG 1 cut(s) 519
MspA1I CMGCKG 1 cut(s) 333
MspI CCGG 8 cut(s) 116, 200, 206, 224, 252, 266, 390, 456
MspR9I CCNGG 2 cut(s) 391, 422
Mva1269I GAATGC 1 cut(s) 457
MvaI CCWGG 1 cut(s) 422
MwoI GCNNNNNNNGC 1 cut(s) 511
NarI GGCGCC 1 cut(s) 203
NciI CCSGG 1 cut(s) 391
NdeII GATC 4 cut(s) 162, 235, 268, 393
NlaIII CATG 1 cut(s) 145
NlaIV GGNNCC 2 cut(s) 204, 426
NmeAIII GCCGAG 1 cut(s) 285
NmuCI GTSAC 1 cut(s) 122
NspV TTCGAA 1 cut(s) 567
PagI TCATGA 1 cut(s) 141
PciSI GCTCTTC 1 cut(s) 140
PcsI WCGNNNNNNNCGW 3 cut(s) 56, 383, 441
PctI GAATGC 1 cut(s) 457
PdmI GAANNNNTTC 1 cut(s) 149
PfeI GAWTC 5 cut(s) 101, 107, 209, 248, 533
PflFI GACNNNGTC 1 cut(s) 50
PfoI TCCNGGA 1 cut(s) 389
PkrI GCNGC 5 cut(s) 66, 220, 335, 343, 576
PleI GAGTC 8 cut(s) 41, 49, 128, 129, 265, 364, 377, 431
PluTI GGCGCC 1 cut(s) 206
PpsI GAGTC 8 cut(s) 41, 49, 128, 129, 265, 364, 377, 431
Ppu21I YACGTR 1 cut(s) 490
PshAI GACNNNNGTC 1 cut(s) 423
Psp124BI GAGCTC 1 cut(s) 306
Psp6I CCWGG 1 cut(s) 420
PspGI CCWGG 1 cut(s) 420
PspN4I GGNNCC 2 cut(s) 204, 426
PspPI GGNCC 1 cut(s) 425
PsuI RGATCY 2 cut(s) 162, 268
PsyI GACNNNGTC 1 cut(s) 50
RseI CAYNNNNRTG 1 cut(s) 519
SacI GAGCTC 1 cut(s) 306
SapI GCTCTTC 1 cut(s) 140
SatI GCNGC 5 cut(s) 65, 219, 334, 342, 575
Sau3AI GATC 4 cut(s) 162, 235, 268, 393
Sau96I GGNCC 1 cut(s) 425
SchI GAGTC 8 cut(s) 41, 50, 128, 130, 266, 365, 377, 431
ScrFI CCNGG 2 cut(s) 391, 422
SduI GDGCHC 2 cut(s) 306, 516
SetI ASST 9 cut(s) 81, 132, 152, 191, 245, 282, 306, 463, 492
SfoI GGCGCC 1 cut(s) 204
SfuI TTCGAA 1 cut(s) 567
SinI GGWCC 1 cut(s) 425
SmiMI CAYNNNNRTG 1 cut(s) 519
Sse9I AATT 1 cut(s) 175
SspDI GGCGCC 1 cut(s) 202
SspMI CTAG 1 cut(s) 284
SstI GAGCTC 1 cut(s) 306
StyD4I CCNGG 2 cut(s) 389, 420
TaiI ACGT 3 cut(s) 191, 245, 492
TaqI TCGA 8 cut(s) 54, 132, 153, 185, 234, 444, 510, 567
TaqII GACCGA 1 cut(s) 40
TasI AATT 1 cut(s) 175
TauI GCSGC 5 cut(s) 67, 221, 336, 344, 577
TfiI GAWTC 5 cut(s) 101, 107, 209, 248, 533
TseFI GTSAC 1 cut(s) 122
Tsp45I GTSAC 1 cut(s) 122
TspDTI ATGAA 2 cut(s) 130, 158
TspGWI ACGGA 1 cut(s) 100
Tth111I GACNNNGTC 1 cut(s) 50
VpaK11BI GGWCC 1 cut(s) 425
XagI CCTNNNNNAGG 1 cut(s) 83
XbaI TCTAGA 1 cut(s) 283
XmnI GAANNNNTTC 1 cut(s) 149
XspI CTAG 1 cut(s) 284
ZraI GACGTC 2 cut(s) 189, 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.