AT3G27230

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012101
Physical Location & Seq
Forward (+)
10053929 .. 10055841
1913 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G27230.1

Sequence Viewer

Length: 1233 bp
ATGGGATCTGTATCTTTAAAGATCGGCGATGGAACAGCCAGATTCCGACGAACATCAATTTGTTCATCCGCCGTGAACCTCCTCATGCTCTTCTCCGTCGTCACCACAAATCTCTTCGCCTTATACGCGTTCTCCTCTCATTCCCAAGCAAACTCGCCACTTCTCCACAGCTCCAACAATATCTCACTCGTTTCTCAACATCTCTCTTTGATTCTCCGAGAAATCGATTCGTCTCAACGCAAGCTTGCTCAGATGGAGAAACAGATGCTTGGTTACGAATCTATTGATATCTCTCGACCTAACATTGTTCCTGAGCTCAAATTGTTCCTCCAGCGTCATCAGCTTCCTTTAGGGAAAGATTCGCGTACCGGGATCACTGAAATGGTGTCTTCCGTTGGACATTCTTGTGGGAAATCTACGGATTTGCTTTCTCAGTATATGTCATATAAGGTGTTTGATAGATGTCCTGATGATTGGAGTCTTGGCCAGAAGTTGATTCTTCGTGCTTGTGAGCCGTTGCCGCGGAGACGGTGTTTGGCGAAAACGGTGCAGAAGCAAGATTTGAGTAAGTCTCCTGATTCGTTATGGAGATCTGTTAGTAACAAGAGTGTTAATTGGAGTGGACTTGGTTGCAAGAGTTTTGATTGCTTGAAAGGTAAGAAACTTAGCAAGGAATGTGTTGGTTGTTTCGATTTAGGGGTTGAGAAAGATAGGTTTGTGAAGGTTAAGGGGAAGAATGATTTCTTGATTGATGATGTGTTGGGTTTAGGAAGTGGGAAAATTCGAATCGGGTTCGATATTAGCGGTGGATCAGGGACTTTCGCAGCGAGAATGGCTGAGAAGAACGTGACTGTAATCACTAACACTTTGAATAATGGTGCTCCGTTTAGTGAGTTTATAGCTGCGAGAGGGCTCTTTCCGTTGTTCTTGAGCTTAGACCATAGATTTCCTTTTCTTGACAATGTGTTTGATCTTATTCATGCTTCTAGCGGATTAGATGTTGAGGGTAAAGCGGAGAAGCTAGAGTTCTTGATGTTCGATCTTGATCGGGTTTTGAAACCCCGTGGATTGTTCTGGTTGGATAACTTCTATTGTGCTAATGATGAGAAGAAGAAAGAACTTACACGTATGATTGAGAGGTTTGGGTATAAGAAGCTTAAATGGGTTATTGGAGAGAAGGCTGATGCACAAGTTTATCTCTCAGCTGTTCTGCAAAAGCCAGTGAGAGCTTGA

Protein Analysis

410

Amino Acids

45.86

Weight (kDa)

9.33

Isoelectric Point (pI)

39.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 258 - 360 2.1e-06 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012101)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27230 AT5G40830 AT5G40830 AT5G40830
fragaria_vesca FvH4_2g38780
malus_domestica MD08G1015600.v1.1 MD15G1014500.v1.1
prunus_persica Prupe.1G368400_v2.0.a1
pyrus_communis pycom15g01260
rosa_chinensis RchiOBHm_Chr6g0302531
rosa_laevigata RLG00000011165
rosa_multiflora Rmu_sc0008826.1_g000003
rosa_roxburghii Rroxscaffold_7G00165650
rosa_rugosa Rorug06G0314700
rosa_samantha Rh6AG428000 Rh6BG474700 Rh6CG440100 Rh6DG427100
rosa_wichuraiana Rw6G037020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 128, 364, 523
AciI CCGC 6 cut(s) 69, 521, 523, 804, 990, 1013
AclWI GGATC 3 cut(s) 13, 380, 817
AcoI YGGCCR 1 cut(s) 484
AcsI RAATTY 1 cut(s) 780
AfaI GTAC 1 cut(s) 367
AflIII ACRYGT 2 cut(s) 126, 1124
AgsI TTSAA 3 cut(s) 652, 871, 1057
Alw21I GWGCWC 2 cut(s) 318, 883
Alw26I GTCTC 3 cut(s) 237, 520, 576
AlwI GGATC 3 cut(s) 13, 380, 817
AoxI GGCC 1 cut(s) 484
ApeKI GCWGC 2 cut(s) 824, 902
ApoI RAATTY 1 cut(s) 780
ArsI GACNNNNNNTTYG 2 cut(s) 950, 982
Asp700I GAANNNNTTC 1 cut(s) 740
AsuC2I CCSGG 1 cut(s) 370
AsuHPI GGTGA 1 cut(s) 94
AsuII TTCGAA 1 cut(s) 784
BalI TGGCCA 1 cut(s) 486
BanII GRGCYC 2 cut(s) 318, 915
BbsI GAAGAC 1 cut(s) 381
Bbv12I GWGCWC 2 cut(s) 318, 883
BbvI GCAGC 2 cut(s) 836, 889
BccI CCATC 2 cut(s) 23, 247
BceAI ACGGC 2 cut(s) 56, 499
BcgI CGANNNNNNTGC 2 cut(s) 529, 563
BcnI CCSGG 1 cut(s) 370
BcoDI GTCTC 3 cut(s) 237, 520, 576
BfaI CTAG 2 cut(s) 987, 1022
BglII AGATCT 1 cut(s) 590
BisI GCNGC 3 cut(s) 521, 825, 903
BlsI GCNGC 3 cut(s) 522, 826, 904
Bme1390I CCNGG 1 cut(s) 370
BmrFI CCNGG 1 cut(s) 370
BmsI GCATC 2 cut(s) 255, 1174
BpiI GAAGAC 1 cut(s) 381
BplI GAGNNNNNCTC 2 cut(s) 556, 588
BpmI CTGGAG 1 cut(s) 314
Bpu10I CCTNAGC 1 cut(s) 312
Bpu14I TTCGAA 1 cut(s) 784
BpuEI CTTGAG 1 cut(s) 949
BpuMI CCSGG 1 cut(s) 370
Bsa29I ATCGAT 1 cut(s) 225
BsaAI YACGTR 1 cut(s) 1127
BsaBI GATNNNNATC 2 cut(s) 10, 1044
BsaJI CCNNGG 2 cut(s) 521, 1063
BsaXI ACNNNNNCTCC 4 cut(s) 116, 146, 517, 547
Bse1I ACTGG 1 cut(s) 1220
Bse8I GATNNNNATC 2 cut(s) 10, 1044
BseCI ATCGAT 1 cut(s) 225
BseDI CCNNGG 2 cut(s) 521, 1063
BseGI GGATG 1 cut(s) 65
BseJI GATNNNNATC 2 cut(s) 10, 1044
BseMII CTCAG 5 cut(s) 263, 303, 446, 828, 1215
BseNI ACTGG 1 cut(s) 1220
BseRI GAGGAG 2 cut(s) 71, 124
BseXI GCAGC 2 cut(s) 836, 889
BsgI GTGCAG 1 cut(s) 569
Bsh1236I CGCG 3 cut(s) 128, 364, 523
BshFI GGCC 1 cut(s) 486
BshVI ATCGAT 1 cut(s) 225
BsiHKAI GWGCWC 2 cut(s) 318, 883
BsiSI CCGG 1 cut(s) 369
BslFI GGGAC 1 cut(s) 829
BsmAI GTCTC 3 cut(s) 237, 520, 576
BsmBI CGTCTC 2 cut(s) 237, 520
BsmFI GGGAC 1 cut(s) 829
BsnI GGCC 1 cut(s) 486
Bsp119I TTCGAA 1 cut(s) 784
Bsp1286I GDGCHC 3 cut(s) 318, 883, 915
Bsp143I GATC 8 cut(s) 5, 21, 372, 590, 809, 970, 1039, 1045
BspACI CCGC 6 cut(s) 69, 521, 523, 804, 990, 1013
BspANI GGCC 1 cut(s) 486
BspCNI CTCAG 5 cut(s) 262, 304, 445, 829, 1214
BspDI ATCGAT 1 cut(s) 225
BspFNI CGCG 3 cut(s) 128, 364, 523
BspPI GGATC 3 cut(s) 13, 380, 817
BspQI GCTCTTC 1 cut(s) 95
BspT104I TTCGAA 1 cut(s) 784
BsrI ACTGG 1 cut(s) 1220
BssECI CCNNGG 2 cut(s) 521, 1063
BssMI GATC 8 cut(s) 5, 21, 372, 590, 809, 970, 1039, 1045
Bst4CI ACNGT 3 cut(s) 531, 547, 853
Bst6I CTCTTC 2 cut(s) 95, 119
BstBAI YACGTR 1 cut(s) 1127
BstBI TTCGAA 1 cut(s) 784
BstC8I GCNNGC 2 cut(s) 242, 246
BstDEI CTNAG 7 cut(s) 249, 312, 432, 665, 837, 934, 1201
BstDSI CCRYGG 2 cut(s) 521, 1063
BstF5I GGATG 1 cut(s) 65
BstFNI CGCG 3 cut(s) 128, 364, 523
BstKTI GATC 8 cut(s) 8, 24, 375, 593, 812, 973, 1042, 1048
BstMAI GTCTC 3 cut(s) 237, 520, 576
BstMBI GATC 8 cut(s) 5, 21, 372, 590, 809, 970, 1039, 1045
BstMWI GCNNNNNNNGC 4 cut(s) 125, 340, 520, 833
BstSCI CCNGG 1 cut(s) 368
BstUI CGCG 3 cut(s) 128, 364, 523
BstV1I GCAGC 2 cut(s) 836, 889
BstV2I GAAGAC 1 cut(s) 381
BstX2I RGATCY 2 cut(s) 5, 590
BstYI RGATCY 2 cut(s) 5, 590
Bsu15I ATCGAT 1 cut(s) 225
BsuRI GGCC 1 cut(s) 486
BsuTUI ATCGAT 1 cut(s) 225
BtgI CCRYGG 2 cut(s) 521, 1063
BtgZI GCGATG 1 cut(s) 42
BtsCI GGATG 1 cut(s) 65
BtsIMutI CAGTG 2 cut(s) 375, 1227
Cac8I GCNNGC 2 cut(s) 242, 246
Cfr42I CCGCGG 1 cut(s) 524
ClaI ATCGAT 1 cut(s) 225
CseI GACGC 1 cut(s) 323
Csp6I GTAC 1 cut(s) 366
CviAII CATG 2 cut(s) 85, 980
CviQI GTAC 1 cut(s) 366
DdeI CTNAG 7 cut(s) 249, 312, 432, 665, 837, 934, 1201
DpnI GATC 8 cut(s) 7, 23, 374, 592, 811, 972, 1041, 1047
DpnII GATC 8 cut(s) 5, 21, 372, 590, 809, 970, 1039, 1045
DraI TTTAAA 1 cut(s) 18
EaeI YGGCCR 1 cut(s) 484
Eam1104I CTCTTC 2 cut(s) 95, 119
EarI CTCTTC 2 cut(s) 95, 119
EciI GGCGGA 1 cut(s) 58
Ecl136II GAGCTC 1 cut(s) 316
Eco24I GRGCYC 2 cut(s) 318, 915
Eco32I GATATC 1 cut(s) 289
Eco53kI GAGCTC 1 cut(s) 316
EcoICRI GAGCTC 1 cut(s) 316
EcoRV GATATC 1 cut(s) 289
EcoT38I GRGCYC 2 cut(s) 318, 915
Esp3I CGTCTC 2 cut(s) 237, 520
FaeI CATG 2 cut(s) 88, 983
FaqI GGGAC 1 cut(s) 829
FatI CATG 2 cut(s) 84, 979
Fnu4HI GCNGC 3 cut(s) 521, 825, 903
FokI GGATG 1 cut(s) 52
FriOI GRGCYC 2 cut(s) 318, 915
Fsp4HI GCNGC 3 cut(s) 521, 825, 903
FspBI CTAG 2 cut(s) 987, 1022
GluI GCNGC 3 cut(s) 521, 825, 903
GsuI CTGGAG 1 cut(s) 314
HaeIII GGCC 1 cut(s) 486
HapII CCGG 1 cut(s) 369
HgaI GACGC 1 cut(s) 323
Hin1II CATG 2 cut(s) 88, 983
HindIII AAGCTT 2 cut(s) 242, 1154
HinfI GANTC 9 cut(s) 42, 211, 227, 278, 359, 478, 496, 578, 786
HpaII CCGG 1 cut(s) 369
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 2 cut(s) 76, 623
Hpy188I TCNGA 3 cut(s) 47, 218, 252
Hpy188III TCNNGA 9 cut(s) 294, 311, 467, 575, 745, 928, 956, 1030, 1043
Hpy8I GTNNAC 2 cut(s) 76, 623
Hpy99I CGWCG 2 cut(s) 51, 101
HpyAV CCTTC 2 cut(s) 715, 1171
HpyCH4III ACNGT 3 cut(s) 531, 547, 853
HpyCH4IV ACGT 2 cut(s) 846, 1126
HpyCH4V TGCA 4 cut(s) 550, 633, 1187, 1213
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 340, 520, 833
HpyF3I CTNAG 7 cut(s) 249, 312, 432, 665, 837, 934, 1201
HpySE526I ACGT 2 cut(s) 846, 1126
Hsp92II CATG 2 cut(s) 88, 983
KspI CCGCGG 1 cut(s) 524
Kzo9I GATC 8 cut(s) 5, 21, 372, 590, 809, 970, 1039, 1045
LguI GCTCTTC 1 cut(s) 95
LmnI GCTCC 2 cut(s) 176, 886
LpnPI CCDG 9 cut(s) 52, 324, 344, 382, 480, 500, 588, 798, 1060
Lsp1109I GCAGC 2 cut(s) 836, 889
LweI GCATC 2 cut(s) 255, 1174
MaeI CTAG 2 cut(s) 987, 1022
MaeII ACGT 2 cut(s) 846, 1126
MaeIII GTNAC 4 cut(s) 100, 272, 599, 847
MalI GATC 8 cut(s) 7, 23, 374, 592, 811, 972, 1041, 1047
MboI GATC 8 cut(s) 5, 21, 372, 590, 809, 970, 1039, 1045
MboII GAAGA 8 cut(s) 82, 106, 381, 491, 745, 853, 1120, 1123
MflI RGATCY 2 cut(s) 5, 590
MhlI GDGCHC 3 cut(s) 318, 883, 915
MlsI TGGCCA 1 cut(s) 486
MluCI AATT 4 cut(s) 57, 320, 613, 780
MluI ACGCGT 1 cut(s) 126
MluNI TGGCCA 1 cut(s) 486
MlyI GAGTC 1 cut(s) 487
MmeI TCCRAC 4 cut(s) 70, 198, 376, 1059
MnlI CCTC 7 cut(s) 89, 92, 145, 338, 902, 997, 1131
Mox20I TGGCCA 1 cut(s) 486
MroXI GAANNNNTTC 1 cut(s) 740
MscI TGGCCA 1 cut(s) 486
MseI TTAA 4 cut(s) 17, 612, 726, 1158
MslI CAYNNNNRTG 2 cut(s) 380, 405
Msp20I TGGCCA 1 cut(s) 486
MspA1I CMGCKG 2 cut(s) 523, 1205
MspI CCGG 1 cut(s) 369
MspR9I CCNGG 1 cut(s) 370
MvnI CGCG 3 cut(s) 128, 364, 523
MwoI GCNNNNNNNGC 4 cut(s) 125, 340, 520, 833
NciI CCSGG 1 cut(s) 370
NdeII GATC 8 cut(s) 5, 21, 372, 590, 809, 970, 1039, 1045
NlaIII CATG 2 cut(s) 88, 983
NmuCI GTSAC 2 cut(s) 100, 847
NspV TTCGAA 1 cut(s) 784
PciSI GCTCTTC 1 cut(s) 95
PdmI GAANNNNTTC 1 cut(s) 740
PfeI GAWTC 8 cut(s) 42, 211, 227, 278, 359, 496, 578, 786
PkrI GCNGC 3 cut(s) 522, 826, 904
PleI GAGTC 1 cut(s) 486
PpsI GAGTC 1 cut(s) 486
Ppu21I YACGTR 1 cut(s) 1127
Psp124BI GAGCTC 1 cut(s) 318
PsuI RGATCY 2 cut(s) 5, 590
PvuII CAGCTG 1 cut(s) 1205
RsaI GTAC 1 cut(s) 367
RsaNI GTAC 1 cut(s) 366
RseI CAYNNNNRTG 2 cut(s) 380, 405
SacI GAGCTC 1 cut(s) 318
SacII CCGCGG 1 cut(s) 524
SapI GCTCTTC 1 cut(s) 95
SaqAI TTAA 4 cut(s) 17, 612, 726, 1158
SatI GCNGC 3 cut(s) 521, 825, 903
Sau3AI GATC 8 cut(s) 5, 21, 372, 590, 809, 970, 1039, 1045
SchI GAGTC 1 cut(s) 487
ScrFI CCNGG 1 cut(s) 370
SduI GDGCHC 3 cut(s) 318, 883, 915
SfaNI GCATC 2 cut(s) 255, 1174
Sfr303I CCGCGG 1 cut(s) 524
SfuI TTCGAA 1 cut(s) 784
SgrBI CCGCGG 1 cut(s) 524
SmiMI CAYNNNNRTG 2 cut(s) 380, 405
SmlI CTYRAG 1 cut(s) 928
SmoI CTYRAG 1 cut(s) 928
Sse9I AATT 4 cut(s) 57, 320, 613, 780
SsiI CCGC 6 cut(s) 69, 521, 523, 804, 990, 1013
SspMI CTAG 2 cut(s) 987, 1022
SstI GAGCTC 1 cut(s) 318
StyD4I CCNGG 1 cut(s) 368
TaaI ACNGT 3 cut(s) 531, 547, 853
TaiI ACGT 2 cut(s) 849, 1129
TaqI TCGA 6 cut(s) 225, 295, 690, 784, 795, 1038
TasI AATT 4 cut(s) 57, 320, 613, 780
TauI GCSGC 1 cut(s) 523
TfiI GAWTC 8 cut(s) 42, 211, 227, 278, 359, 496, 578, 786
Tru1I TTAA 4 cut(s) 17, 612, 726, 1158
Tru9I TTAA 4 cut(s) 17, 612, 726, 1158
TscAI CASTG 2 cut(s) 382, 1227
TseFI GTSAC 2 cut(s) 100, 847
TseI GCWGC 2 cut(s) 824, 902
Tsp45I GTSAC 2 cut(s) 100, 847
TspDTI ATGAA 2 cut(s) 54, 968
TspGWI ACGGA 5 cut(s) 85, 382, 434, 873, 909
TspRI CASTG 2 cut(s) 382, 1227
XapI RAATTY 1 cut(s) 780
XmnI GAANNNNTTC 1 cut(s) 740
XspI CTAG 2 cut(s) 987, 1022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.