Rroxscaffold_7G00165650

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0012101
Physical Location & Seq
Reverse (-)
7328241 .. 7329510
1270 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00165650.1

Sequence Viewer

Length: 1089 bp
ATGGGCTCCGTCTCTCTGAAGATCGGCGACGGCACCGCCAGATTCCGACGCGCCACCCTGTGCTCCTCCGCCGTCAACATCCTCATGCTCTTCTCCGTCCTCACCACCAACCTCTTCGCCCTCTACGCCTTCACCTCCTCCTCCCCAAAAGACCAACAACACGCCCAGCACCTCCTCCACCACACCCAGAAGAACATCTCCCTCATCTCCGAGCAGGTCTCCCTCATCCTCCGCGAGATCGACTCCTCCCAGAAGAAGCTCGCCCAGATGGAGAAGGACCTCTTGGGCTACCAAACCATCGATCTTTCCCGCCCCAATACGCCTAATGAGCTCAAGAAATTCCTCCAGCATCACACCCTTCCTCTCGGCAAAGACTCGAGAACCGGGATCACCGAGATGGTGGCCTCCGTGGGACATTCCTGCGAGAAGTCCGCGGATTTGTTGTCCCAGTACATGAACTACAAGGTCTCTGGGCCCTGCCCCGACGACTGGAGCCTGGCCCAGAGGCTGATTCTTCGCGGATGCGAGCCTCTGCCGAGGAGGAGGTGCTTTGCCAAAACCGTGCCCAAGGTTGGTTTAACTACTTTTCCGCTTTCGCTTTGGAAAAATGTTAGTGCTAAGAGTGTTACTTGGAGCGGTCTAGGGTGTGGGTCTGGTACATTTGCTGCTAGAATGGCGGAGAGGAATGTGACTGTGATCACCAACACTTTGAACATTGATGCCCCGTATAGTGAGTTCATAGCTGCTAGAGGGCTGTTTCCGCTGTTTTTGAGCTTGGATCATAGATTTCCTTTCTATGATAATGTGTTTGATTTGGTTCATGCTGCGAGCGGATTGGATGTTGGTGGAAAACCGGACAAGTTTGAGTTCTTGATGTTTGATATCGATCGGATCTTGAGGCCTGGAGGTTTGTTTTGGTTAGACAACTTTCATTGTGCCAATGAAGAGAGGAAAAAGGACTTAACCAGGTTAATTGAGAGGTTTGGATATAAAAAGCTGAAATGGGTTGTTGCAGTAGATGCTGCATCAGGGTCAAGCAAATTCGAGGTTTACTTGTCCGCAGTTCTGCAAAAACCAGTAAGAGTATGA

Protein Analysis

362

Amino Acids

40.51

Weight (kDa)

9.3

Isoelectric Point (pI)

39.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 215 - 307 1.3e-06 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012101)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27230 AT5G40830 AT5G40830 AT5G40830
fragaria_vesca FvH4_2g38780
malus_domestica MD08G1015600.v1.1 MD15G1014500.v1.1
prunus_persica Prupe.1G368400_v2.0.a1
pyrus_communis pycom15g01260
rosa_chinensis RchiOBHm_Chr6g0302531
rosa_laevigata RLG00000011165
rosa_multiflora Rmu_sc0008826.1_g000003
rosa_roxburghii Rroxscaffold_7G00165650
rosa_rugosa Rorug06G0314700
rosa_samantha Rh6AG428000 Rh6BG474700 Rh6CG440100 Rh6DG427100
rosa_wichuraiana Rw6G037020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 205
AccB1I GGYRCC 1 cut(s) 32
AccBSI CCGCTC 2 cut(s) 636, 831
AccII CGCG 4 cut(s) 51, 234, 434, 519
AclWI GGATC 3 cut(s) 395, 786, 899
AcsI RAATTY 2 cut(s) 338, 1040
AcuI CTGAAG 1 cut(s) 38
AdeI CACNNNGTG 1 cut(s) 60
AfaI GTAC 2 cut(s) 452, 658
AfiI CCNNNNNNNGG 2 cut(s) 489, 572
AgsI TTSAA 1 cut(s) 712
AjnI CCWGG 3 cut(s) 495, 901, 965
AjuI GAANNNNNNNTTGG 2 cut(s) 266, 298
AluBI AGCT 5 cut(s) 259, 331, 743, 774, 997
AluI AGCT 5 cut(s) 259, 331, 743, 774, 997
Alw21I GWGCWC 2 cut(s) 65, 333
Alw26I GTCTC 3 cut(s) 16, 223, 472
AlwI GGATC 3 cut(s) 395, 786, 899
AlwNI CAGNNNCTG 1 cut(s) 508
Ama87I CYCGRG 1 cut(s) 376
AoxI GGCC 4 cut(s) 402, 473, 498, 899
ApaI GGGCCC 1 cut(s) 477
ApeKI GCWGC 4 cut(s) 665, 743, 824, 1022
ApoI RAATTY 2 cut(s) 338, 1040
AspLEI GCGC 1 cut(s) 53
AspS9I GGNCC 4 cut(s) 277, 473, 474, 499
AsuC2I CCSGG 1 cut(s) 385
AsuHPI GGTGA 4 cut(s) 94, 124, 382, 691
AvaI CYCGRG 1 cut(s) 376
AvaII GGWCC 1 cut(s) 277
BaeGI GKGCMC 2 cut(s) 477, 567
BanI GGYRCC 1 cut(s) 32
BanII GRGCYC 3 cut(s) 8, 333, 477
Bbv12I GWGCWC 2 cut(s) 65, 333
BbvI GCAGC 4 cut(s) 652, 730, 811, 1009
BccI CCATC 3 cut(s) 262, 305, 391
BceAI ACGGC 2 cut(s) 46, 56
BciT130I CCWGG 3 cut(s) 497, 903, 967
BclI TGATCA 1 cut(s) 696
BcnI CCSGG 1 cut(s) 385
BcoDI GTCTC 3 cut(s) 16, 223, 472
BfaI CTAG 3 cut(s) 641, 669, 747
BfuAI ACCTGC 1 cut(s) 205
BisI GCNGC 4 cut(s) 666, 744, 825, 1023
BlsI GCNGC 4 cut(s) 667, 745, 826, 1024
Bme1390I CCNGG 4 cut(s) 385, 497, 903, 967
Bme18I GGWCC 1 cut(s) 277
BmeT110I CYCGRG 1 cut(s) 376
BmgT120I GGNCC 4 cut(s) 277, 473, 474, 499
BmiI GGNNCC 4 cut(s) 7, 34, 475, 494
BmrFI CCNGG 4 cut(s) 385, 497, 903, 967
BmrI ACTGGG 1 cut(s) 442
BmsI GCATC 5 cut(s) 358, 512, 709, 1009, 1034
BmuI ACTGGG 1 cut(s) 442
BplI GAGNNNNNCTC 4 cut(s) 203, 235, 227, 259
BpmI CTGGAG 3 cut(s) 329, 511, 924
BpuEI CTTGAG 2 cut(s) 317, 916
BpuMI CCSGG 1 cut(s) 385
Bsa29I ATCGAT 2 cut(s) 300, 885
BsaBI GATNNNNATC 1 cut(s) 885
BsaI GGTCTC 2 cut(s) 223, 472
BsaJI CCNNGG 4 cut(s) 408, 432, 536, 567
BsaWI WCCGGW 1 cut(s) 853
Bsc4I CCNNNNNNNGG 2 cut(s) 489, 572
Bse1I ACTGG 3 cut(s) 448, 494, 1076
Bse8I GATNNNNATC 1 cut(s) 885
BseBI CCWGG 3 cut(s) 497, 903, 967
BseCI ATCGAT 2 cut(s) 300, 885
BseDI CCNNGG 4 cut(s) 408, 432, 536, 567
BseGI GGATG 4 cut(s) 78, 225, 527, 844
BseJI GATNNNNATC 1 cut(s) 885
BseLI CCNNNNNNNGG 2 cut(s) 489, 572
BseNI ACTGG 3 cut(s) 448, 494, 1076
BseRI GAGGAG 7 cut(s) 55, 127, 130, 164, 235, 553, 556
BseSI GKGCMC 2 cut(s) 477, 567
BseXI GCAGC 4 cut(s) 652, 730, 811, 1009
BseYI CCCAGC 1 cut(s) 165
Bsh1236I CGCG 4 cut(s) 51, 234, 434, 519
Bsh1285I CGRYCG 1 cut(s) 889
BshFI GGCC 4 cut(s) 404, 475, 500, 901
BshNI GGYRCC 1 cut(s) 32
BshVI ATCGAT 2 cut(s) 300, 885
BsiEI CGRYCG 1 cut(s) 889
BsiHKAI GWGCWC 2 cut(s) 65, 333
BsiHKCI CYCGRG 1 cut(s) 376
BsiSI CCGG 2 cut(s) 384, 854
BslFI GGGAC 2 cut(s) 426, 430
BslI CCNNNNNNNGG 2 cut(s) 489, 572
BsmAI GTCTC 3 cut(s) 16, 223, 472
BsmBI CGTCTC 1 cut(s) 16
BsmFI GGGAC 2 cut(s) 426, 430
BsnI GGCC 4 cut(s) 404, 475, 500, 901
Bso31I GGTCTC 2 cut(s) 223, 472
BsoBI CYCGRG 1 cut(s) 376
Bsp120I GGGCCC 1 cut(s) 473
Bsp1286I GDGCHC 5 cut(s) 8, 65, 333, 477, 567
Bsp143I GATC 8 cut(s) 21, 237, 301, 387, 696, 778, 886, 891
BspANI GGCC 4 cut(s) 404, 475, 500, 901
BspDI ATCGAT 2 cut(s) 300, 885
BspFNI CGCG 4 cut(s) 51, 234, 434, 519
BspLI GGNNCC 4 cut(s) 7, 34, 475, 494
BspMI ACCTGC 1 cut(s) 205
BspPI GGATC 3 cut(s) 395, 786, 899
BspQI GCTCTTC 1 cut(s) 95
BspT107I GGYRCC 1 cut(s) 32
BspTNI GGTCTC 2 cut(s) 223, 472
BsrBI CCGCTC 2 cut(s) 636, 831
BsrI ACTGG 3 cut(s) 448, 494, 1076
BssECI CCNNGG 4 cut(s) 408, 432, 536, 567
BssMI GATC 8 cut(s) 21, 237, 301, 387, 696, 778, 886, 891
BssT1I CCWWGG 1 cut(s) 567
Bst2UI CCWGG 3 cut(s) 497, 903, 967
Bst4CI ACNGT 2 cut(s) 562, 694
Bst6I CTCTTC 3 cut(s) 95, 119, 939
BstAPI GCANNNNNTGC 1 cut(s) 1019
BstC8I GCNNGC 3 cut(s) 261, 527, 829
BstDEI CTNAG 1 cut(s) 618
BstDSI CCRYGG 2 cut(s) 408, 432
BstF5I GGATG 4 cut(s) 78, 225, 527, 844
BstFNI CGCG 4 cut(s) 51, 234, 434, 519
BstHHI GCGC 1 cut(s) 53
BstKTI GATC 8 cut(s) 24, 240, 304, 390, 699, 781, 889, 894
BstMAI GTCTC 3 cut(s) 16, 223, 472
BstMBI GATC 8 cut(s) 21, 237, 301, 387, 696, 778, 886, 891
BstMCI CGRYCG 1 cut(s) 889
BstMWI GCNNNNNNNGC 5 cut(s) 125, 328, 674, 760, 1019
BstNI CCWGG 3 cut(s) 497, 903, 967
BstSCI CCNGG 4 cut(s) 383, 495, 901, 965
BstSLI GKGCMC 2 cut(s) 477, 567
BstUI CGCG 4 cut(s) 51, 234, 434, 519
BstV1I GCAGC 4 cut(s) 652, 730, 811, 1009
BstX2I RGATCY 1 cut(s) 891
BstYI RGATCY 1 cut(s) 891
Bsu15I ATCGAT 2 cut(s) 300, 885
BsuRI GGCC 4 cut(s) 404, 475, 500, 901
BsuTUI ATCGAT 2 cut(s) 300, 885
BtgI CCRYGG 2 cut(s) 408, 432
BtsCI GGATG 4 cut(s) 78, 225, 527, 844
BveI ACCTGC 1 cut(s) 205
Cac8I GCNNGC 3 cut(s) 261, 527, 829
CaiI CAGNNNCTG 1 cut(s) 508
CfoI GCGC 1 cut(s) 53
Cfr13I GGNCC 4 cut(s) 277, 473, 474, 499
Cfr42I CCGCGG 1 cut(s) 435
ClaI ATCGAT 2 cut(s) 300, 885
CseI GACGC 1 cut(s) 57
CsiI ACCWGGT 1 cut(s) 965
Csp6I GTAC 2 cut(s) 451, 657
CviAII CATG 3 cut(s) 85, 454, 821
CviQI GTAC 2 cut(s) 451, 657
DdeI CTNAG 1 cut(s) 618
DpnI GATC 8 cut(s) 23, 239, 303, 389, 698, 780, 888, 893
DpnII GATC 8 cut(s) 21, 237, 301, 387, 696, 778, 886, 891
DraIII CACNNNGTG 1 cut(s) 60
Eam1104I CTCTTC 3 cut(s) 95, 119, 939
EarI CTCTTC 3 cut(s) 95, 119, 939
EciI GGCGGA 2 cut(s) 58, 692
Ecl136II GAGCTC 1 cut(s) 331
Eco130I CCWWGG 1 cut(s) 567
Eco147I AGGCCT 1 cut(s) 901
Eco24I GRGCYC 3 cut(s) 8, 333, 477
Eco31I GGTCTC 2 cut(s) 223, 472
Eco32I GATATC 1 cut(s) 883
Eco47I GGWCC 1 cut(s) 277
Eco53kI GAGCTC 1 cut(s) 331
Eco57I CTGAAG 1 cut(s) 38
Eco88I CYCGRG 1 cut(s) 376
EcoICRI GAGCTC 1 cut(s) 331
EcoO109I RGGNCCY 2 cut(s) 277, 474
EcoRII CCWGG 3 cut(s) 495, 901, 965
EcoRV GATATC 1 cut(s) 883
EcoT14I CCWWGG 1 cut(s) 567
EcoT38I GRGCYC 3 cut(s) 8, 333, 477
ErhI CCWWGG 1 cut(s) 567
Esp3I CGTCTC 1 cut(s) 16
FaeI CATG 3 cut(s) 88, 457, 824
FaiI YATR 9 cut(s) 86, 455, 729, 740, 783, 798, 822, 990, 1087
FalI AAGNNNNNCTT 2 cut(s) 266, 298
FaqI GGGAC 2 cut(s) 426, 430
FatI CATG 3 cut(s) 84, 453, 820
FauI CCCGC 1 cut(s) 317
FbaI TGATCA 1 cut(s) 696
Fnu4HI GCNGC 4 cut(s) 666, 744, 825, 1023
FokI GGATG 4 cut(s) 65, 212, 534, 851
FriOI GRGCYC 3 cut(s) 8, 333, 477
Fsp4HI GCNGC 4 cut(s) 666, 744, 825, 1023
FspBI CTAG 3 cut(s) 641, 669, 747
GlaI GCGC 1 cut(s) 52
GluI GCNGC 4 cut(s) 666, 744, 825, 1023
GsaI CCCAGC 1 cut(s) 169
GsuI CTGGAG 3 cut(s) 329, 511, 924
HaeIII GGCC 4 cut(s) 404, 475, 500, 901
HapII CCGG 2 cut(s) 384, 854
HgaI GACGC 1 cut(s) 57
HhaI GCGC 1 cut(s) 53
Hin1II CATG 3 cut(s) 88, 457, 824
Hin6I GCGC 1 cut(s) 51
HinP1I GCGC 1 cut(s) 51
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HinfI GANTC 4 cut(s) 42, 242, 374, 511
HpaII CCGG 2 cut(s) 384, 854
HphI GGTGA 4 cut(s) 94, 124, 382, 691
Hpy166II GTNNAC 2 cut(s) 76, 1051
Hpy188I TCNGA 4 cut(s) 18, 47, 211, 891
Hpy188III TCNNGA 4 cut(s) 334, 378, 871, 895
Hpy8I GTNNAC 2 cut(s) 76, 1051
Hpy99I CGWCG 3 cut(s) 32, 51, 488
HpyAV CCTTC 3 cut(s) 139, 268, 368
HpyCH4III ACNGT 2 cut(s) 562, 694
HpyCH4V TGCA 3 cut(s) 1013, 1025, 1069
HpyF10VI GCNNNNNNNGC 5 cut(s) 125, 328, 674, 760, 1019
HpyF3I CTNAG 1 cut(s) 618
Hsp92II CATG 3 cut(s) 88, 457, 824
HspAI GCGC 1 cut(s) 51
Ksp22I TGATCA 1 cut(s) 696
KspI CCGCGG 1 cut(s) 435
Kzo9I GATC 8 cut(s) 21, 237, 301, 387, 696, 778, 886, 891
LguI GCTCTTC 1 cut(s) 95
LmnI GCTCC 4 cut(s) 11, 68, 492, 633
Lsp1109I GCAGC 4 cut(s) 652, 730, 811, 1009
LweI GCATC 5 cut(s) 358, 512, 709, 1009, 1034
MabI ACCWGGT 1 cut(s) 965
MaeI CTAG 3 cut(s) 641, 669, 747
MaeIII GTNAC 2 cut(s) 625, 688
MalI GATC 8 cut(s) 23, 239, 303, 389, 698, 780, 888, 893
MbiI CCGCTC 2 cut(s) 636, 831
MboI GATC 8 cut(s) 21, 237, 301, 387, 696, 778, 886, 891
MboII GAAGA 7 cut(s) 31, 82, 106, 202, 265, 506, 956
MflI RGATCY 1 cut(s) 891
MhlI GDGCHC 5 cut(s) 8, 65, 333, 477, 567
MluCI AATT 3 cut(s) 338, 972, 1040
MlyI GAGTC 2 cut(s) 236, 368
MmeI TCCRAC 1 cut(s) 70
MseI TTAA 3 cut(s) 578, 962, 971
MslI CAYNNNNRTG 2 cut(s) 83, 395
MspA1I CMGCKG 2 cut(s) 434, 763
MspI CCGG 2 cut(s) 384, 854
MspR9I CCNGG 4 cut(s) 385, 497, 903, 967
MvaI CCWGG 3 cut(s) 497, 903, 967
MvnI CGCG 4 cut(s) 51, 234, 434, 519
MwoI GCNNNNNNNGC 5 cut(s) 125, 328, 674, 760, 1019
NciI CCSGG 1 cut(s) 385
NdeII GATC 8 cut(s) 21, 237, 301, 387, 696, 778, 886, 891
NlaIII CATG 3 cut(s) 88, 457, 824
NlaIV GGNNCC 4 cut(s) 7, 34, 475, 494
NmeAIII GCCGAG 2 cut(s) 345, 561
NmuCI GTSAC 1 cut(s) 688
PaeR7I CTCGAG 1 cut(s) 376
PceI AGGCCT 1 cut(s) 901
PciSI GCTCTTC 1 cut(s) 95
PfeI GAWTC 2 cut(s) 42, 511
PkrI GCNGC 4 cut(s) 667, 745, 826, 1024
Ple19I CGATCG 1 cut(s) 889
PleI GAGTC 2 cut(s) 236, 368
PpsI GAGTC 2 cut(s) 236, 368
PpuMI RGGWCCY 1 cut(s) 277
Psp124BI GAGCTC 1 cut(s) 333
Psp5II RGGWCCY 1 cut(s) 277
Psp6I CCWGG 3 cut(s) 495, 901, 965
PspFI CCCAGC 1 cut(s) 165
PspGI CCWGG 3 cut(s) 495, 901, 965
PspN4I GGNNCC 4 cut(s) 7, 34, 475, 494
PspOMI GGGCCC 1 cut(s) 473
PspPI GGNCC 4 cut(s) 277, 473, 474, 499
PspPPI RGGWCCY 1 cut(s) 277
PsrI GAACNNNNNNTAC 2 cut(s) 719, 751
PstNI CAGNNNCTG 1 cut(s) 508
PsuI RGATCY 1 cut(s) 891
PvuI CGATCG 1 cut(s) 889
RsaI GTAC 2 cut(s) 452, 658
RsaNI GTAC 2 cut(s) 451, 657
RseI CAYNNNNRTG 2 cut(s) 83, 395
SacI GAGCTC 1 cut(s) 333
SacII CCGCGG 1 cut(s) 435
SapI GCTCTTC 1 cut(s) 95
SaqAI TTAA 3 cut(s) 578, 962, 971
SatI GCNGC 4 cut(s) 666, 744, 825, 1023
Sau3AI GATC 8 cut(s) 21, 237, 301, 387, 696, 778, 886, 891
Sau96I GGNCC 4 cut(s) 277, 473, 474, 499
SchI GAGTC 2 cut(s) 236, 368
ScrFI CCNGG 4 cut(s) 385, 497, 903, 967
SduI GDGCHC 5 cut(s) 8, 65, 333, 477, 567
SexAI ACCWGGT 1 cut(s) 965
SfaNI GCATC 5 cut(s) 358, 512, 709, 1009, 1034
Sfr274I CTCGAG 1 cut(s) 376
Sfr303I CCGCGG 1 cut(s) 435
SgrBI CCGCGG 1 cut(s) 435
SinI GGWCC 1 cut(s) 277
SlaI CTCGAG 1 cut(s) 376
SmiMI CAYNNNNRTG 2 cut(s) 83, 395
SmlI CTYRAG 3 cut(s) 332, 376, 895
SmoI CTYRAG 3 cut(s) 332, 376, 895
Sse9I AATT 3 cut(s) 338, 972, 1040
SseBI AGGCCT 1 cut(s) 901
SspMI CTAG 3 cut(s) 641, 669, 747
SstI GAGCTC 1 cut(s) 333
StuI AGGCCT 1 cut(s) 901
StyD4I CCNGG 4 cut(s) 383, 495, 901, 965
StyI CCWWGG 1 cut(s) 567
TaaI ACNGT 2 cut(s) 562, 694
TaqI TCGA 5 cut(s) 240, 300, 377, 885, 1044
TasI AATT 3 cut(s) 338, 972, 1040
TatI WGTACW 1 cut(s) 450
TfiI GAWTC 2 cut(s) 42, 511
Tru1I TTAA 3 cut(s) 578, 962, 971
Tru9I TTAA 3 cut(s) 578, 962, 971
TseFI GTSAC 1 cut(s) 688
TseI GCWGC 4 cut(s) 665, 743, 824, 1022
Tsp45I GTSAC 1 cut(s) 688
TspDTI ATGAA 5 cut(s) 470, 727, 809, 920, 957
TspGWI ACGGA 2 cut(s) 85, 397
VpaK11BI GGWCC 1 cut(s) 277
XapI RAATTY 2 cut(s) 338, 1040
XhoI CTCGAG 1 cut(s) 376
XspI CTAG 3 cut(s) 641, 669, 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.