MD08G1015600.v1.1

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012101
Physical Location & Seq
Reverse (-)
1224576 .. 1225885
1310 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1015600.v1.1.491

Sequence Viewer

Length: 1266 bp
ATGGGCTCTGTTTCTCTCAAGATCGGCGACGGAAGCGCCAGATTCCGAAGAGCATCGCTCTGTTCCTCTGCCGTCAACCTCCTCATGCTGTTTTCCGTCATCACCACCAACCTCTTCGCGCTCTACGCCTTCACATACTCCCCGAAAGACCACCAAACATACCGTCTCCTCCACCACACCCAGAAGAACATCTCCCTCATCTCCGAGCAGGTCGCTCTGATCCTCCGCGAGATTGATTCGTCGCAAAAGAAGCTTGCCCAGATGGAAAAGGAGCTCCTCGGCTACGAAAGCATCGATCTTTCTAATCCCAATATCGCGAACGAGCTCAAAGTCTTCCTCCAGCACCACTCGCTCCCTCTCGGCAAAGATTCGAAAACCGGGATCACGGAAATGGTGGCTTCCGTCGGCCATTCCTGCGAGAAATCCGCCGGCTTGCTGTCACAGTACATGAACTACAAGGTCTCCGGGCCTTGTCCCGACGATTGGAGCCTTGCCCAGAAGCTGATTCTGCGCGGATGCGAGCCTCTGCCGCGACGGAGGTGCTTTGCCAAGGCGGTTCCTAAGGTGGGTTTAGACCCTTTTCCGATTTCGCTTTGGAAACCTGTTAGTGATAAGATTGTGAGTTGGAGCGGCCTTGGATGCAAGAAATTTGAATGCTTGAATGCTAAGAAATTGAGTAGGGATTGCGTTGGTTGTTTCGATTTGGTTAACGGGTCCGAAAACGAGAGATTTGTCAAGGCTAGGAGCAAGAATGATTTCCTCATTGATGATGTTTTAGCTTTGGGGAGTGGAGGAATCCGAATAGGGTTTGATATTGGAGGTGGGTCTGGTACCTTCGCTGCTAGAATGGCAGAGAGGAACGTGACTGTGATCACCAACACTCTGAATATCGAAGCCCCGTTTAACGAATTCATAGCTGCAAGAGGGCTTTTCCCTCTGTTTTTGAGCTTGGATCATACGTTTCCCTTCTACGATAATGTGTTCGATTTGGTCCATGCCGCCAGTGGATTGGATGTTGGAGGAAAACCGGAAAAGTTCGAGTTCTTGATGTTTGACATCGATCGCATTTTGAGGGCGGGAGGTTTGTTATGGCTGGACAACTTTTATTGCTCGGATGATGAAAAGAAGAGAGATTTAACCAGGTTGATCGAGCGGTTTGGATATAAAAAGCTCAAATGGGTTGTCGGGGAGAAGGCAGATACGGCAGGGTCAGGCAAATTGGAGATTTATTTGTCTGCCGTTTTGCAGAAACCGGTTAGAGTCTGA

Protein Analysis

422

Amino Acids

46.8

Weight (kDa)

8.77

Isoelectric Point (pI)

38.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 263 - 365 5.5e-06 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012101)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27230 AT5G40830 AT5G40830 AT5G40830
fragaria_vesca FvH4_2g38780
malus_domestica MD08G1015600.v1.1 MD15G1014500.v1.1
prunus_persica Prupe.1G368400_v2.0.a1
pyrus_communis pycom15g01260
rosa_chinensis RchiOBHm_Chr6g0302531
rosa_laevigata RLG00000011165
rosa_multiflora Rmu_sc0008826.1_g000003
rosa_roxburghii Rroxscaffold_7G00165650
rosa_rugosa Rorug06G0314700
rosa_samantha Rh6AG428000 Rh6BG474700 Rh6CG440100 Rh6DG427100
rosa_wichuraiana Rw6G037020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 199
Acc65I GGTACC 1 cut(s) 830
AccB1I GGYRCC 1 cut(s) 830
AccBSI CCGCTC 2 cut(s) 630, 1153
AccII CGCG 5 cut(s) 119, 228, 317, 513, 532
AciI CCGC 9 cut(s) 226, 426, 513, 530, 554, 630, 999, 1076, 1153
AclWI GGATC 3 cut(s) 214, 389, 960
AcoI YGGCCR 1 cut(s) 406
AcsI RAATTY 2 cut(s) 647, 908
AfaI GTAC 2 cut(s) 446, 832
AfiI CCNNNNNNNGG 2 cut(s) 483, 566
AgeI ACCGGT 1 cut(s) 1252
AgsI TTSAA 2 cut(s) 653, 661
AjnI CCWGG 1 cut(s) 1139
AluBI AGCT 8 cut(s) 253, 274, 325, 502, 779, 917, 948, 1171
AluI AGCT 8 cut(s) 253, 274, 325, 502, 779, 917, 948, 1171
Alw21I GWGCWC 2 cut(s) 276, 327
Alw26I GTCTC 2 cut(s) 170, 466
AlwI GGATC 3 cut(s) 214, 389, 960
AlwNI CAGNNNCTG 1 cut(s) 502
AoxI GGCC 3 cut(s) 406, 467, 631
ApeKI GCWGC 2 cut(s) 839, 917
ApoI RAATTY 2 cut(s) 647, 908
ArsI GACNNNNNNTTYG 4 cut(s) 148, 180, 1167, 1199
AsiGI ACCGGT 1 cut(s) 1252
Asp700I GAANNNNTTC 1 cut(s) 755
Asp718I GGTACC 1 cut(s) 830
AspLEI GCGC 3 cut(s) 38, 121, 513
AspS9I GGNCC 3 cut(s) 467, 714, 991
AsuC2I CCSGG 2 cut(s) 379, 466
AsuHPI GGTGA 2 cut(s) 94, 865
AsuII TTCGAA 1 cut(s) 371
AvaII GGWCC 2 cut(s) 714, 991
AxyI CCTNAGG 1 cut(s) 561
BanI GGYRCC 1 cut(s) 830
BanII GRGCYC 3 cut(s) 8, 276, 327
BbsI GAAGAC 1 cut(s) 325
Bbv12I GWGCWC 2 cut(s) 276, 327
BbvI GCAGC 2 cut(s) 826, 904
BccI CCATC 1 cut(s) 256
BceAI ACGGC 3 cut(s) 56, 1218, 1223
BcgI CGANNNNNNTGC 2 cut(s) 522, 556
BciT130I CCWGG 1 cut(s) 1141
BclI TGATCA 1 cut(s) 870
BcnI CCSGG 2 cut(s) 379, 466
BcoDI GTCTC 2 cut(s) 170, 466
BfaI CTAG 2 cut(s) 741, 843
BfoI RGCGCY 1 cut(s) 39
BfuAI ACCTGC 1 cut(s) 199
BisI GCNGC 5 cut(s) 530, 631, 840, 918, 999
BlsI GCNGC 5 cut(s) 531, 632, 841, 919, 1000
Bme1390I CCNGG 3 cut(s) 379, 466, 1141
Bme18I GGWCC 2 cut(s) 714, 991
BmgT120I GGNCC 3 cut(s) 467, 714, 991
BmiI GGNNCC 4 cut(s) 488, 558, 715, 832
BmrFI CCNGG 3 cut(s) 379, 466, 1141
BmsI GCATC 4 cut(s) 62, 300, 506, 629
BpiI GAAGAC 1 cut(s) 325
BplI GAGNNNNNCTC 2 cut(s) 42, 74
BpmI CTGGAG 1 cut(s) 323
Bpu14I TTCGAA 1 cut(s) 371
BpuMI CCSGG 2 cut(s) 379, 466
Bsa29I ATCGAT 2 cut(s) 294, 1059
BsaI GGTCTC 1 cut(s) 466
BsaJI CCNNGG 3 cut(s) 277, 549, 634
BsaWI WCCGGW 2 cut(s) 1027, 1252
BsaXI ACNNNNNCTCC 2 cut(s) 446, 476
Bsc4I CCNNNNNNNGG 2 cut(s) 483, 566
Bse118I RCCGGY 2 cut(s) 428, 1252
Bse1I ACTGG 1 cut(s) 1002
Bse21I CCTNAGG 1 cut(s) 561
BseBI CCWGG 1 cut(s) 1141
BseCI ATCGAT 2 cut(s) 294, 1059
BseDI CCNNGG 3 cut(s) 277, 549, 634
BseGI GGATG 4 cut(s) 521, 644, 1018, 1120
BseLI CCNNNNNNNGG 2 cut(s) 483, 566
BseNI ACTGG 1 cut(s) 1002
BseRI GAGGAG 3 cut(s) 71, 158, 266
BseXI GCAGC 2 cut(s) 826, 904
Bsh1236I CGCG 5 cut(s) 119, 228, 317, 513, 532
Bsh1285I CGRYCG 1 cut(s) 1063
BshFI GGCC 3 cut(s) 408, 469, 633
BshNI GGYRCC 1 cut(s) 830
BshTI ACCGGT 1 cut(s) 1252
BshVI ATCGAT 2 cut(s) 294, 1059
BsiEI CGRYCG 1 cut(s) 1063
BsiHKAI GWGCWC 2 cut(s) 276, 327
BsiSI CCGG 5 cut(s) 378, 429, 465, 1028, 1253
BslFI GGGAC 1 cut(s) 459
BslI CCNNNNNNNGG 2 cut(s) 483, 566
BsmAI GTCTC 2 cut(s) 170, 466
BsmBI CGTCTC 1 cut(s) 170
BsmFI GGGAC 1 cut(s) 459
BsmI GAATGC 2 cut(s) 659, 667
BsnI GGCC 3 cut(s) 408, 469, 633
Bso31I GGTCTC 1 cut(s) 466
Bsp119I TTCGAA 1 cut(s) 371
Bsp1286I GDGCHC 3 cut(s) 8, 276, 327
Bsp143I GATC 8 cut(s) 21, 219, 295, 381, 870, 952, 1060, 1146
Bsp68I TCGCGA 1 cut(s) 317
BspACI CCGC 9 cut(s) 226, 426, 513, 530, 554, 630, 999, 1076, 1153
BspANI GGCC 3 cut(s) 408, 469, 633
BspDI ATCGAT 2 cut(s) 294, 1059
BspFNI CGCG 5 cut(s) 119, 228, 317, 513, 532
BspLI GGNNCC 4 cut(s) 488, 558, 715, 832
BspMI ACCTGC 1 cut(s) 199
BspPI GGATC 3 cut(s) 214, 389, 960
BspQI GCTCTTC 1 cut(s) 43
BspT104I TTCGAA 1 cut(s) 371
BspT107I GGYRCC 1 cut(s) 830
BspTNI GGTCTC 1 cut(s) 466
BsrBI CCGCTC 2 cut(s) 630, 1153
BsrFI RCCGGY 2 cut(s) 428, 1252
BsrI ACTGG 1 cut(s) 1002
BssAI RCCGGY 2 cut(s) 428, 1252
BssECI CCNNGG 3 cut(s) 277, 549, 634
BssMI GATC 8 cut(s) 21, 219, 295, 381, 870, 952, 1060, 1146
BssT1I CCWWGG 2 cut(s) 549, 634
Bst2UI CCWGG 1 cut(s) 1141
Bst4CI ACNGT 3 cut(s) 164, 444, 868
Bst6I CTCTTC 3 cut(s) 43, 119, 1121
BstBI TTCGAA 1 cut(s) 371
BstC8I GCNNGC 4 cut(s) 255, 430, 434, 521
BstDEI CTNAG 2 cut(s) 561, 666
BstF5I GGATG 4 cut(s) 521, 644, 1018, 1120
BstFNI CGCG 5 cut(s) 119, 228, 317, 513, 532
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 3 cut(s) 38, 121, 513
BstKTI GATC 8 cut(s) 24, 222, 298, 384, 873, 955, 1063, 1149
BstMAI GTCTC 2 cut(s) 170, 466
BstMBI GATC 8 cut(s) 21, 219, 295, 381, 870, 952, 1060, 1146
BstMCI CGRYCG 1 cut(s) 1063
BstNI CCWGG 1 cut(s) 1141
BstSCI CCNGG 3 cut(s) 377, 464, 1139
BstUI CGCG 5 cut(s) 119, 228, 317, 513, 532
BstV1I GCAGC 2 cut(s) 826, 904
BstV2I GAAGAC 1 cut(s) 325
BstXI CCANNNNNNTGG 1 cut(s) 1009
Bsu15I ATCGAT 2 cut(s) 294, 1059
Bsu36I CCTNAGG 1 cut(s) 561
BsuRI GGCC 3 cut(s) 408, 469, 633
BsuTUI ATCGAT 2 cut(s) 294, 1059
BtgZI GCGATG 1 cut(s) 39
BtsCI GGATG 4 cut(s) 521, 644, 1018, 1120
BtsIMutI CAGTG 1 cut(s) 1009
BtuMI TCGCGA 1 cut(s) 317
BveI ACCTGC 1 cut(s) 199
Cac8I GCNNGC 4 cut(s) 255, 430, 434, 521
CaiI CAGNNNCTG 1 cut(s) 502
CfoI GCGC 3 cut(s) 38, 121, 513
Cfr10I RCCGGY 2 cut(s) 428, 1252
Cfr13I GGNCC 3 cut(s) 467, 714, 991
ClaI ATCGAT 2 cut(s) 294, 1059
CsiI ACCWGGT 1 cut(s) 1139
Csp6I GTAC 2 cut(s) 445, 831
CspAI ACCGGT 1 cut(s) 1252
CviAII CATG 3 cut(s) 85, 448, 995
CviQI GTAC 2 cut(s) 445, 831
DdeI CTNAG 2 cut(s) 561, 666
DpnI GATC 8 cut(s) 23, 221, 297, 383, 872, 954, 1062, 1148
DpnII GATC 8 cut(s) 21, 219, 295, 381, 870, 952, 1060, 1146
EaeI YGGCCR 1 cut(s) 406
Eam1104I CTCTTC 3 cut(s) 43, 119, 1121
EarI CTCTTC 3 cut(s) 43, 119, 1121
EciI GGCGGA 1 cut(s) 415
Ecl136II GAGCTC 2 cut(s) 274, 325
Eco130I CCWWGG 2 cut(s) 549, 634
Eco24I GRGCYC 3 cut(s) 8, 276, 327
Eco31I GGTCTC 1 cut(s) 466
Eco47I GGWCC 2 cut(s) 714, 991
Eco53kI GAGCTC 2 cut(s) 274, 325
Eco81I CCTNAGG 1 cut(s) 561
EcoICRI GAGCTC 2 cut(s) 274, 325
EcoRI GAATTC 1 cut(s) 908
EcoRII CCWGG 1 cut(s) 1139
EcoT14I CCWWGG 2 cut(s) 549, 634
EcoT38I GRGCYC 3 cut(s) 8, 276, 327
ErhI CCWWGG 2 cut(s) 549, 634
Esp3I CGTCTC 1 cut(s) 170
FaeI CATG 3 cut(s) 88, 451, 998
FaiI YATR 9 cut(s) 86, 136, 160, 449, 914, 957, 996, 1090, 1164
FaqI GGGAC 1 cut(s) 459
FatI CATG 3 cut(s) 84, 447, 994
FauI CCCGC 1 cut(s) 1069
FbaI TGATCA 1 cut(s) 870
Fnu4HI GCNGC 5 cut(s) 530, 631, 840, 918, 999
FokI GGATG 4 cut(s) 528, 651, 1025, 1127
FriOI GRGCYC 3 cut(s) 8, 276, 327
Fsp4HI GCNGC 5 cut(s) 530, 631, 840, 918, 999
FspBI CTAG 2 cut(s) 741, 843
GlaI GCGC 3 cut(s) 37, 120, 512
GluI GCNGC 5 cut(s) 530, 631, 840, 918, 999
GsuI CTGGAG 1 cut(s) 323
HaeII RGCGCY 1 cut(s) 39
HaeIII GGCC 3 cut(s) 408, 469, 633
HapII CCGG 5 cut(s) 378, 429, 465, 1028, 1253
HhaI GCGC 3 cut(s) 38, 121, 513
Hin1II CATG 3 cut(s) 88, 451, 998
Hin6I GCGC 3 cut(s) 36, 119, 511
HinP1I GCGC 3 cut(s) 36, 119, 511
HincII GTYRAC 2 cut(s) 76, 709
HindII GTYRAC 2 cut(s) 76, 709
HindIII AAGCTT 1 cut(s) 251
HinfI GANTC 6 cut(s) 42, 236, 368, 505, 795, 1260
HpaI GTTAAC 1 cut(s) 709
HpaII CCGG 5 cut(s) 378, 429, 465, 1028, 1253
HphI GGTGA 2 cut(s) 94, 865
Hpy166II GTNNAC 2 cut(s) 76, 709
Hpy188I TCNGA 9 cut(s) 47, 205, 219, 585, 718, 800, 885, 1114, 1265
Hpy188III TCNNGA 4 cut(s) 19, 316, 476, 1045
Hpy8I GTNNAC 2 cut(s) 76, 709
Hpy99I CGWCG 5 cut(s) 32, 244, 407, 482, 537
HpyAV CCTTC 4 cut(s) 139, 844, 976, 1186
HpyCH4III ACNGT 3 cut(s) 164, 444, 868
HpyCH4IV ACGT 2 cut(s) 861, 959
HpyCH4V TGCA 3 cut(s) 642, 920, 1246
HpyF3I CTNAG 2 cut(s) 561, 666
HpySE526I ACGT 2 cut(s) 861, 959
Hsp92II CATG 3 cut(s) 88, 451, 998
HspAI GCGC 3 cut(s) 36, 119, 511
KpnI GGTACC 1 cut(s) 834
KroI GCCGGC 1 cut(s) 428
KroNI GCCGGC 1 cut(s) 430
Ksp22I TGATCA 1 cut(s) 870
KspAI GTTAAC 1 cut(s) 709
Kzo9I GATC 8 cut(s) 21, 219, 295, 381, 870, 952, 1060, 1146
LguI GCTCTTC 1 cut(s) 43
LmnI GCTCC 6 cut(s) 271, 279, 357, 486, 627, 744
Lsp1109I GCAGC 2 cut(s) 826, 904
LweI GCATC 4 cut(s) 62, 300, 506, 629
MabI ACCWGGT 1 cut(s) 1139
MaeI CTAG 2 cut(s) 741, 843
MaeII ACGT 2 cut(s) 861, 959
MaeIII GTNAC 2 cut(s) 438, 862
MalI GATC 8 cut(s) 23, 221, 297, 383, 872, 954, 1062, 1148
MbiI CCGCTC 2 cut(s) 630, 1153
MboI GATC 8 cut(s) 21, 219, 295, 381, 870, 952, 1060, 1146
MboII GAAGA 5 cut(s) 60, 106, 196, 325, 1138
MhlI GDGCHC 3 cut(s) 8, 276, 327
MluCI AATT 4 cut(s) 647, 671, 908, 1217
MmeI TCCRAC 2 cut(s) 605, 997
MroNI GCCGGC 1 cut(s) 428
MroXI GAANNNNTTC 1 cut(s) 755
MseI TTAA 3 cut(s) 708, 903, 1136
MslI CAYNNNNRTG 1 cut(s) 389
MspI CCGG 5 cut(s) 378, 429, 465, 1028, 1253
MspR9I CCNGG 3 cut(s) 379, 466, 1141
Mva1269I GAATGC 2 cut(s) 659, 667
MvaI CCWGG 1 cut(s) 1141
MvnI CGCG 5 cut(s) 119, 228, 317, 513, 532
NaeI GCCGGC 1 cut(s) 430
NciI CCSGG 2 cut(s) 379, 466
NdeII GATC 8 cut(s) 21, 219, 295, 381, 870, 952, 1060, 1146
NgoMIV GCCGGC 1 cut(s) 428
NlaIII CATG 3 cut(s) 88, 451, 998
NlaIV GGNNCC 4 cut(s) 488, 558, 715, 832
NmeAIII GCCGAG 2 cut(s) 258, 339
NmuCI GTSAC 2 cut(s) 438, 862
NruI TCGCGA 1 cut(s) 317
NspV TTCGAA 1 cut(s) 371
PciSI GCTCTTC 1 cut(s) 43
PcsI WCGNNNNNNNCGW 1 cut(s) 291
PctI GAATGC 2 cut(s) 659, 667
PdiI GCCGGC 1 cut(s) 430
PdmI GAANNNNTTC 1 cut(s) 755
PfeI GAWTC 5 cut(s) 42, 236, 368, 505, 795
PinAI ACCGGT 1 cut(s) 1252
PkrI GCNGC 5 cut(s) 531, 632, 841, 919, 1000
Ple19I CGATCG 1 cut(s) 1063
Psp124BI GAGCTC 2 cut(s) 276, 327
Psp6I CCWGG 1 cut(s) 1139
PspGI CCWGG 1 cut(s) 1139
PspN4I GGNNCC 4 cut(s) 488, 558, 715, 832
PspPI GGNCC 3 cut(s) 467, 714, 991
PstNI CAGNNNCTG 1 cut(s) 502
PvuI CGATCG 1 cut(s) 1063
RruI TCGCGA 1 cut(s) 317
RsaI GTAC 2 cut(s) 446, 832
RsaNI GTAC 2 cut(s) 445, 831
RseI CAYNNNNRTG 1 cut(s) 389
SacI GAGCTC 2 cut(s) 276, 327
SapI GCTCTTC 1 cut(s) 43
SaqAI TTAA 3 cut(s) 708, 903, 1136
SatI GCNGC 5 cut(s) 530, 631, 840, 918, 999
Sau3AI GATC 8 cut(s) 21, 219, 295, 381, 870, 952, 1060, 1146
Sau96I GGNCC 3 cut(s) 467, 714, 991
ScrFI CCNGG 3 cut(s) 379, 466, 1141
SduI GDGCHC 3 cut(s) 8, 276, 327
SexAI ACCWGGT 1 cut(s) 1139
SfaNI GCATC 4 cut(s) 62, 300, 506, 629
SfuI TTCGAA 1 cut(s) 371
SinI GGWCC 2 cut(s) 714, 991
SmiMI CAYNNNNRTG 1 cut(s) 389
SmlI CTYRAG 1 cut(s) 17
SmoI CTYRAG 1 cut(s) 17
Sse9I AATT 4 cut(s) 647, 671, 908, 1217
SsiI CCGC 9 cut(s) 226, 426, 513, 530, 554, 630, 999, 1076, 1153
SspMI CTAG 2 cut(s) 741, 843
SstI GAGCTC 2 cut(s) 276, 327
StyD4I CCNGG 3 cut(s) 377, 464, 1139
StyI CCWWGG 2 cut(s) 549, 634
TaaI ACNGT 3 cut(s) 164, 444, 868
TaiI ACGT 2 cut(s) 864, 962
TaqI TCGA 8 cut(s) 294, 371, 699, 891, 984, 1038, 1059, 1149
TasI AATT 4 cut(s) 647, 671, 908, 1217
TatI WGTACW 1 cut(s) 444
TauI GCSGC 3 cut(s) 532, 633, 1001
TfiI GAWTC 5 cut(s) 42, 236, 368, 505, 795
Tru1I TTAA 3 cut(s) 708, 903, 1136
Tru9I TTAA 3 cut(s) 708, 903, 1136
TscAI CASTG 1 cut(s) 1009
TseFI GTSAC 2 cut(s) 438, 862
TseI GCWGC 2 cut(s) 839, 917
Tsp45I GTSAC 2 cut(s) 438, 862
TspDTI ATGAA 3 cut(s) 464, 901, 1134
TspGWI ACGGA 5 cut(s) 45, 85, 391, 401, 550
TspRI CASTG 1 cut(s) 1009
VpaK11BI GGWCC 2 cut(s) 714, 991
XapI RAATTY 2 cut(s) 647, 908
XcmI CCANNNNNNNNNTGG 1 cut(s) 1001
XmnI GAANNNNTTC 1 cut(s) 755
XspI CTAG 2 cut(s) 741, 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.