MD15G1014500.v1.1

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012101
Physical Location & Seq
Reverse (-)
850482 .. 852296
1815 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1014500.v1.1.491

Sequence Viewer

Length: 1194 bp
ATGGGCTCTGTTTCTCTCAAGATCGGCGACGGAACCGCCAGATTCCGAAGAGCGTCGCTCTGTTCCTCCGCCGTCAACCTCCTCATGCTCTTCTCTGTCATCACTACCAACCTCTTCGCCCTCTACGCCTTCACATCCGCCCCGAAAGACCACCAAACGTACCACCTCCTCCACCACACCCAGAAGAACATCTCCCTCATCTCCGAGCAGGTCGGTCTGATCCTCCGCGAGATCGATTCGTCGCAAAAGAAGCTTGCCCAGATGGAAAAGGAGCTCCTTGGATACAAAAGCATCGATCTTTCGAAACGCAATGTCGCGAACGAGCTCAAAGTCTTCCTCCAGCACCACCAGCTCCCTCTCGGAAAAGATTCGAAAACCGGGATCACGGAAATGGTGGCTTCCGTCGGCCATTCCTGCGAGAAATCCGCCGACTTGCTTTCACAGTACATGAACTACAGGGTCTCCGGGCCCTGCCCCGACGATTGGAGCCTTGCCCAGAAGCTGATTCTGCGGGGATGCGAGCCTCTGCCGCGACGGAGGTGCTTTGCCAAGACAGTTACTAAGGTGGGTTTACACCCTTTACCGATTTCGCTTTGGAAACCTGTTAGTGATAAGATTGTGAGTTGGAGTGGCCTTGAATGCAAGAAATTTGAATGCTTGAATGTTAAGAAATTGAGTAGGGATTGCGTTGGTTGTTTCGATTTGGTTAACGGGTCCGAAACCCAGAGAATAGGATTTGATATTGGAGGTGGGTCTGGTACCTTTGCTGCTAGAATGGCAGAGAGGAACATGACTGTGATCACCAACACATTGAATATCGGAGCCCCGTTTAGCGAATTCATAGCAGCAAGAGGGCTTTTCCCTCTGTTTTTGAGCTTGGATCATAGGTTTCCTTTCTATGATAATGTGTTCGATATGGTTCATGCCGCTAGTGGATTGGATGTTGCGGCAAAACCGGAAAAGTTTGAGTTCTTGATGTTTGACATCGACCGCATTTTGAGGCCTGGAGGTCTGTTTTGGTTGGACAACTTTTATGGCTCCGATGATGAAAAGAAGAGGGATTTAACCAGATTGATCGAGCGGTTTGGATATAAAAAGCTGAAATGGGTTGTCGGGGAGAAGGCGGAGGCAGCAGGGTCAGGCAAATCAGAGATCTATTTGTCTGCCGTTTTGCAGAAACCAGTTAGAGTATGA

Protein Analysis

398

Amino Acids

44.41

Weight (kDa)

9.13

Isoelectric Point (pI)

38.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 242 - 341 7.6e-07 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012101)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27230 AT5G40830 AT5G40830 AT5G40830
fragaria_vesca FvH4_2g38780
malus_domestica MD08G1015600.v1.1 MD15G1014500.v1.1
prunus_persica Prupe.1G368400_v2.0.a1
pyrus_communis pycom15g01260
rosa_chinensis RchiOBHm_Chr6g0302531
rosa_laevigata RLG00000011165
rosa_multiflora Rmu_sc0008826.1_g000003
rosa_roxburghii Rroxscaffold_7G00165650
rosa_rugosa Rorug06G0314700
rosa_samantha Rh6AG428000 Rh6BG474700 Rh6CG440100 Rh6DG427100
rosa_wichuraiana Rw6G037020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 199
Acc65I GGTACC 1 cut(s) 758
AccB1I GGYRCC 1 cut(s) 758
AccBSI CCGCTC 1 cut(s) 1081
AccII CGCG 3 cut(s) 228, 317, 532
AclWI GGATC 3 cut(s) 214, 389, 888
AcoI YGGCCR 1 cut(s) 406
AcsI RAATTY 2 cut(s) 647, 836
AfaI GTAC 3 cut(s) 161, 446, 760
AfiI CCNNNNNNNGG 1 cut(s) 483
AgsI TTSAA 4 cut(s) 638, 653, 661, 814
AjnI CCWGG 1 cut(s) 1003
AluBI AGCT 7 cut(s) 253, 274, 325, 352, 502, 876, 1099
AluI AGCT 7 cut(s) 253, 274, 325, 352, 502, 876, 1099
Alw21I GWGCWC 2 cut(s) 276, 327
Alw26I GTCTC 1 cut(s) 466
AlwI GGATC 3 cut(s) 214, 389, 888
AlwNI CAGNNNCTG 1 cut(s) 502
AoxI GGCC 4 cut(s) 406, 467, 631, 1001
ApaI GGGCCC 1 cut(s) 471
ApeKI GCWGC 3 cut(s) 767, 845, 1130
ApoI RAATTY 2 cut(s) 647, 836
Asp700I GAANNNNTTC 1 cut(s) 367
Asp718I GGTACC 1 cut(s) 758
AspS9I GGNCC 3 cut(s) 467, 468, 714
AsuC2I CCSGG 2 cut(s) 379, 466
AsuHPI GGTGA 1 cut(s) 793
AsuII TTCGAA 2 cut(s) 302, 371
AvaII GGWCC 1 cut(s) 714
BaeGI GKGCMC 1 cut(s) 471
BanI GGYRCC 1 cut(s) 758
BanII GRGCYC 5 cut(s) 8, 276, 327, 471, 826
BbsI GAAGAC 1 cut(s) 325
Bbv12I GWGCWC 2 cut(s) 276, 327
BbvI GCAGC 3 cut(s) 754, 857, 1142
BccI CCATC 1 cut(s) 256
BceAI ACGGC 2 cut(s) 56, 1151
BcgI CGANNNNNNTGC 2 cut(s) 522, 556
BciT130I CCWGG 1 cut(s) 1005
BciVI GTATCC 1 cut(s) 275
BclI TGATCA 1 cut(s) 798
BcnI CCSGG 2 cut(s) 379, 466
BcoDI GTCTC 1 cut(s) 466
BfaI CTAG 2 cut(s) 771, 930
BfmI CTRYAG 1 cut(s) 454
BfuAI ACCTGC 1 cut(s) 199
BfuI GTATCC 1 cut(s) 275
BglII AGATCT 1 cut(s) 1152
BisI GCNGC 6 cut(s) 530, 768, 846, 927, 948, 1131
BlsI GCNGC 6 cut(s) 531, 769, 847, 928, 949, 1132
Bme1390I CCNGG 3 cut(s) 379, 466, 1005
Bme18I GGWCC 1 cut(s) 714
BmgT120I GGNCC 3 cut(s) 467, 468, 714
BmiI GGNNCC 7 cut(s) 34, 469, 488, 715, 760, 823, 1039
BmrFI CCNGG 3 cut(s) 379, 466, 1005
BmsI GCATC 2 cut(s) 300, 506
BpiI GAAGAC 1 cut(s) 325
BplI GAGNNNNNCTC 2 cut(s) 42, 74
BpmI CTGGAG 2 cut(s) 323, 1026
Bpu14I TTCGAA 2 cut(s) 302, 371
BpuMI CCSGG 2 cut(s) 379, 466
Bsa29I ATCGAT 2 cut(s) 234, 294
BsaI GGTCTC 1 cut(s) 466
BsaJI CCNNGG 1 cut(s) 277
BsaWI WCCGGW 1 cut(s) 955
BsaXI ACNNNNNCTCC 2 cut(s) 446, 476
Bsc4I CCNNNNNNNGG 1 cut(s) 483
Bse1I ACTGG 1 cut(s) 1181
Bse3DI GCAATG 1 cut(s) 316
BseBI CCWGG 1 cut(s) 1005
BseCI ATCGAT 2 cut(s) 234, 294
BseDI CCNNGG 1 cut(s) 277
BseGI GGATG 3 cut(s) 134, 521, 946
BseLI CCNNNNNNNGG 1 cut(s) 483
BseMI GCAATG 1 cut(s) 316
BseNI ACTGG 1 cut(s) 1181
BseRI GAGGAG 2 cut(s) 71, 158
BseSI GKGCMC 1 cut(s) 471
BseXI GCAGC 3 cut(s) 754, 857, 1142
Bsh1236I CGCG 3 cut(s) 228, 317, 532
Bsh1285I CGRYCG 1 cut(s) 991
BshFI GGCC 4 cut(s) 408, 469, 633, 1003
BshNI GGYRCC 1 cut(s) 758
BshVI ATCGAT 2 cut(s) 234, 294
BsiEI CGRYCG 1 cut(s) 991
BsiHKAI GWGCWC 2 cut(s) 276, 327
BsiSI CCGG 3 cut(s) 378, 465, 956
BslI CCNNNNNNNGG 1 cut(s) 483
BsmAI GTCTC 1 cut(s) 466
BsmI GAATGC 2 cut(s) 644, 659
BsnI GGCC 4 cut(s) 408, 469, 633, 1003
Bso31I GGTCTC 1 cut(s) 466
Bsp119I TTCGAA 2 cut(s) 302, 371
Bsp120I GGGCCC 1 cut(s) 467
Bsp1286I GDGCHC 5 cut(s) 8, 276, 327, 471, 826
Bsp143I GATC 9 cut(s) 21, 219, 231, 295, 381, 798, 880, 1074, 1152
Bsp68I TCGCGA 1 cut(s) 317
BspANI GGCC 4 cut(s) 408, 469, 633, 1003
BspDI ATCGAT 2 cut(s) 234, 294
BspFNI CGCG 3 cut(s) 228, 317, 532
BspLI GGNNCC 7 cut(s) 34, 469, 488, 715, 760, 823, 1039
BspMI ACCTGC 1 cut(s) 199
BspPI GGATC 3 cut(s) 214, 389, 888
BspQI GCTCTTC 2 cut(s) 43, 95
BspT104I TTCGAA 2 cut(s) 302, 371
BspT107I GGYRCC 1 cut(s) 758
BspTNI GGTCTC 1 cut(s) 466
BsrBI CCGCTC 1 cut(s) 1081
BsrDI GCAATG 1 cut(s) 316
BsrI ACTGG 1 cut(s) 1181
BssECI CCNNGG 1 cut(s) 277
BssMI GATC 9 cut(s) 21, 219, 231, 295, 381, 798, 880, 1074, 1152
BssT1I CCWWGG 1 cut(s) 277
Bst2UI CCWGG 1 cut(s) 1005
Bst4CI ACNGT 3 cut(s) 444, 556, 796
Bst6I CTCTTC 4 cut(s) 43, 95, 119, 1049
BstBI TTCGAA 2 cut(s) 302, 371
BstC8I GCNNGC 2 cut(s) 255, 521
BstDEI CTNAG 1 cut(s) 561
BstF5I GGATG 3 cut(s) 134, 521, 946
BstFNI CGCG 3 cut(s) 228, 317, 532
BstKTI GATC 9 cut(s) 24, 222, 234, 298, 384, 801, 883, 1077, 1155
BstMAI GTCTC 1 cut(s) 466
BstMBI GATC 9 cut(s) 21, 219, 231, 295, 381, 798, 880, 1074, 1152
BstMCI CGRYCG 1 cut(s) 991
BstMWI GCNNNNNNNGC 9 cut(s) 125, 250, 349, 414, 508, 529, 639, 776, 1130
BstNI CCWGG 1 cut(s) 1005
BstSCI CCNGG 3 cut(s) 377, 464, 1003
BstSFI CTRYAG 1 cut(s) 454
BstSLI GKGCMC 1 cut(s) 471
BstUI CGCG 3 cut(s) 228, 317, 532
BstV1I GCAGC 3 cut(s) 754, 857, 1142
BstV2I GAAGAC 1 cut(s) 325
BstX2I RGATCY 1 cut(s) 1152
BstYI RGATCY 1 cut(s) 1152
Bsu15I ATCGAT 2 cut(s) 234, 294
BsuI GTATCC 1 cut(s) 275
BsuRI GGCC 4 cut(s) 408, 469, 633, 1003
BsuTUI ATCGAT 2 cut(s) 234, 294
BtsCI GGATG 3 cut(s) 134, 521, 946
BtuMI TCGCGA 1 cut(s) 317
BveI ACCTGC 1 cut(s) 199
Cac8I GCNNGC 2 cut(s) 255, 521
CaiI CAGNNNCTG 1 cut(s) 502
Cfr13I GGNCC 3 cut(s) 467, 468, 714
ClaI ATCGAT 2 cut(s) 234, 294
CseI GACGC 1 cut(s) 42
Csp6I GTAC 3 cut(s) 160, 445, 759
CviAII CATG 4 cut(s) 85, 448, 790, 923
CviQI GTAC 3 cut(s) 160, 445, 759
DdeI CTNAG 1 cut(s) 561
DpnI GATC 9 cut(s) 23, 221, 233, 297, 383, 800, 882, 1076, 1154
DpnII GATC 9 cut(s) 21, 219, 231, 295, 381, 798, 880, 1074, 1152
EaeI YGGCCR 1 cut(s) 406
Eam1104I CTCTTC 4 cut(s) 43, 95, 119, 1049
EarI CTCTTC 4 cut(s) 43, 95, 119, 1049
EciI GGCGGA 4 cut(s) 58, 127, 415, 1139
Ecl136II GAGCTC 2 cut(s) 274, 325
Eco130I CCWWGG 1 cut(s) 277
Eco147I AGGCCT 1 cut(s) 1003
Eco24I GRGCYC 5 cut(s) 8, 276, 327, 471, 826
Eco31I GGTCTC 1 cut(s) 466
Eco47I GGWCC 1 cut(s) 714
Eco53kI GAGCTC 2 cut(s) 274, 325
EcoICRI GAGCTC 2 cut(s) 274, 325
EcoO109I RGGNCCY 1 cut(s) 468
EcoRI GAATTC 1 cut(s) 836
EcoRII CCWGG 1 cut(s) 1003
EcoT14I CCWWGG 1 cut(s) 277
EcoT38I GRGCYC 5 cut(s) 8, 276, 327, 471, 826
ErhI CCWWGG 1 cut(s) 277
FaeI CATG 4 cut(s) 88, 451, 793, 926
FatI CATG 4 cut(s) 84, 447, 789, 922
FauI CCCGC 1 cut(s) 504
FbaI TGATCA 1 cut(s) 798
Fnu4HI GCNGC 6 cut(s) 530, 768, 846, 927, 948, 1131
FokI GGATG 3 cut(s) 121, 528, 953
FriOI GRGCYC 5 cut(s) 8, 276, 327, 471, 826
Fsp4HI GCNGC 6 cut(s) 530, 768, 846, 927, 948, 1131
FspBI CTAG 2 cut(s) 771, 930
GluI GCNGC 6 cut(s) 530, 768, 846, 927, 948, 1131
GsuI CTGGAG 2 cut(s) 323, 1026
HaeIII GGCC 4 cut(s) 408, 469, 633, 1003
HapII CCGG 3 cut(s) 378, 465, 956
HgaI GACGC 1 cut(s) 42
Hin1II CATG 4 cut(s) 88, 451, 793, 926
HincII GTYRAC 2 cut(s) 76, 709
HindII GTYRAC 2 cut(s) 76, 709
HindIII AAGCTT 1 cut(s) 251
HinfI GANTC 4 cut(s) 42, 236, 368, 505
HpaI GTTAAC 1 cut(s) 709
HpaII CCGG 3 cut(s) 378, 465, 956
HphI GGTGA 1 cut(s) 793
Hpy166II GTNNAC 3 cut(s) 76, 572, 709
Hpy188I TCNGA 8 cut(s) 47, 205, 219, 362, 718, 821, 1042, 1150
Hpy188III TCNNGA 3 cut(s) 19, 316, 973
Hpy8I GTNNAC 3 cut(s) 76, 572, 709
Hpy99I CGWCG 6 cut(s) 32, 58, 244, 407, 482, 537
HpyAV CCTTC 2 cut(s) 139, 1114
HpyCH4III ACNGT 3 cut(s) 444, 556, 796
HpyCH4IV ACGT 1 cut(s) 158
HpyCH4V TGCA 2 cut(s) 642, 1174
HpyF10VI GCNNNNNNNGC 9 cut(s) 125, 250, 349, 414, 508, 529, 639, 776, 1130
HpyF3I CTNAG 1 cut(s) 561
HpySE526I ACGT 1 cut(s) 158
Hsp92II CATG 4 cut(s) 88, 451, 793, 926
KpnI GGTACC 1 cut(s) 762
Ksp22I TGATCA 1 cut(s) 798
KspAI GTTAAC 1 cut(s) 709
Kzo9I GATC 9 cut(s) 21, 219, 231, 295, 381, 798, 880, 1074, 1152
LguI GCTCTTC 2 cut(s) 43, 95
LmnI GCTCC 6 cut(s) 271, 279, 357, 486, 821, 1043
Lsp1109I GCAGC 3 cut(s) 754, 857, 1142
LweI GCATC 2 cut(s) 300, 506
MaeI CTAG 2 cut(s) 771, 930
MaeII ACGT 1 cut(s) 158
MaeIII GTNAC 1 cut(s) 556
MalI GATC 9 cut(s) 23, 221, 233, 297, 383, 800, 882, 1076, 1154
MbiI CCGCTC 1 cut(s) 1081
MboI GATC 9 cut(s) 21, 219, 231, 295, 381, 798, 880, 1074, 1152
MboII GAAGA 6 cut(s) 60, 82, 106, 196, 325, 1066
MflI RGATCY 1 cut(s) 1152
MhlI GDGCHC 5 cut(s) 8, 276, 327, 471, 826
MluCI AATT 3 cut(s) 647, 671, 836
MmeI TCCRAC 2 cut(s) 605, 1002
MroXI GAANNNNTTC 1 cut(s) 367
MseI TTAA 3 cut(s) 666, 708, 1064
MslI CAYNNNNRTG 2 cut(s) 389, 794
MspI CCGG 3 cut(s) 378, 465, 956
MspR9I CCNGG 3 cut(s) 379, 466, 1005
Mva1269I GAATGC 2 cut(s) 644, 659
MvaI CCWGG 1 cut(s) 1005
MvnI CGCG 3 cut(s) 228, 317, 532
MwoI GCNNNNNNNGC 9 cut(s) 125, 250, 349, 414, 508, 529, 639, 776, 1130
NciI CCSGG 2 cut(s) 379, 466
NdeII GATC 9 cut(s) 21, 219, 231, 295, 381, 798, 880, 1074, 1152
NlaIII CATG 4 cut(s) 88, 451, 793, 926
NlaIV GGNNCC 7 cut(s) 34, 469, 488, 715, 760, 823, 1039
NruI TCGCGA 1 cut(s) 317
NspV TTCGAA 2 cut(s) 302, 371
PceI AGGCCT 1 cut(s) 1003
PciSI GCTCTTC 2 cut(s) 43, 95
PctI GAATGC 2 cut(s) 644, 659
PdmI GAANNNNTTC 1 cut(s) 367
PfeI GAWTC 4 cut(s) 42, 236, 368, 505
PkrI GCNGC 6 cut(s) 531, 769, 847, 928, 949, 1132
Psp124BI GAGCTC 2 cut(s) 276, 327
Psp6I CCWGG 1 cut(s) 1003
PspGI CCWGG 1 cut(s) 1003
PspN4I GGNNCC 7 cut(s) 34, 469, 488, 715, 760, 823, 1039
PspOMI GGGCCC 1 cut(s) 467
PspPI GGNCC 3 cut(s) 467, 468, 714
PstNI CAGNNNCTG 1 cut(s) 502
PsuI RGATCY 1 cut(s) 1152
RruI TCGCGA 1 cut(s) 317
RsaI GTAC 3 cut(s) 161, 446, 760
RsaNI GTAC 3 cut(s) 160, 445, 759
RseI CAYNNNNRTG 2 cut(s) 389, 794
SacI GAGCTC 2 cut(s) 276, 327
SapI GCTCTTC 2 cut(s) 43, 95
SaqAI TTAA 3 cut(s) 666, 708, 1064
SatI GCNGC 6 cut(s) 530, 768, 846, 927, 948, 1131
Sau3AI GATC 9 cut(s) 21, 219, 231, 295, 381, 798, 880, 1074, 1152
Sau96I GGNCC 3 cut(s) 467, 468, 714
ScrFI CCNGG 3 cut(s) 379, 466, 1005
SduI GDGCHC 5 cut(s) 8, 276, 327, 471, 826
SfaNI GCATC 2 cut(s) 300, 506
SfcI CTRYAG 1 cut(s) 454
SfuI TTCGAA 2 cut(s) 302, 371
SinI GGWCC 1 cut(s) 714
SmiMI CAYNNNNRTG 2 cut(s) 389, 794
SmlI CTYRAG 1 cut(s) 17
SmoI CTYRAG 1 cut(s) 17
Sse9I AATT 3 cut(s) 647, 671, 836
SseBI AGGCCT 1 cut(s) 1003
SspMI CTAG 2 cut(s) 771, 930
SstI GAGCTC 2 cut(s) 276, 327
StuI AGGCCT 1 cut(s) 1003
StyD4I CCNGG 3 cut(s) 377, 464, 1003
StyI CCWWGG 1 cut(s) 277
TaaI ACNGT 3 cut(s) 444, 556, 796
TaiI ACGT 1 cut(s) 161
TaqI TCGA 8 cut(s) 234, 294, 302, 371, 699, 912, 987, 1077
TaqII GACCGA 1 cut(s) 203
TasI AATT 3 cut(s) 647, 671, 836
TatI WGTACW 1 cut(s) 444
TauI GCSGC 3 cut(s) 532, 929, 950
TfiI GAWTC 4 cut(s) 42, 236, 368, 505
Tru1I TTAA 3 cut(s) 666, 708, 1064
Tru9I TTAA 3 cut(s) 666, 708, 1064
TseI GCWGC 3 cut(s) 767, 845, 1130
TspDTI ATGAA 4 cut(s) 464, 829, 911, 1062
TspGWI ACGGA 4 cut(s) 45, 391, 401, 550
VpaK11BI GGWCC 1 cut(s) 714
XapI RAATTY 2 cut(s) 647, 836
XmnI GAANNNNTTC 1 cut(s) 367
XspI CTAG 2 cut(s) 771, 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.