AT5G40830

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012101
Physical Location & Seq
Reverse (-)
16354362 .. 16356341
1980 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G40830.2

Sequence Viewer

Length: 1245 bp
ATGGGATCTGTATCGCTGAAGATTGGCGATGGAACCGCAAGATTCAAGCGATCAACGCTTTTTTCTTCAGCTATCAACCTCCTTATGCTTTTCTCTATAGTCACTACAAATCTCTTTGCACTATACGCCTTCTCGTCTCGGTCTCAATCCCACACGCCACACCCTCTTCATTCCAACAATGTCTCTTTGGTTTCTCAACATCTCTCACTCATCCTCAGAGAAATCGATTCCTCCCACCACACACTCACTCAGATGGAGAAACAGATTATTGGTTATGAATCTCTCGATCTCTCGCAACAAGAAGTTCCACAAGAGCTTAAACTTTTCCTTCAACAACATCAGCTTCCTCTTGGTAAAGATTCTCGTACTGGTATCACACAAATGGTTGCATCTGTTGGACATTCCTGTGAAATGTCTCTAGATTTGTTATCTCAGTATATGTCGTATAATGTCTTTGAAAAATGTCCTGATGATTGGAGCCTAGCTCAGAAGTTGATTCTCCGTGCTTGTGAACCGTTGCCACGCCGTCGATGTTTGGCTAAAACGGTGCATAAACCAGGTCTCGCTTTGTTTCCTGATTCTTTGTGGAGACCAGTTGGTAACAGTAGTGTTAACTGGAGCGGTCTTGGCTGCAAAAGTTTCGAATGCTTGAAAGGTAAGAAACTGAGCCGGGATTGTGTTGGTTGCTTTGATCTAGCTACTAGTCATGAGAAAGATAGGTTTGTTAAGGTTAATGGGAAGACTGATTTCCTGATAGATGATGTTTTGGATTTAGGCGATGGGAAAATCCGAATAGGATTCGATATTAGCAGTGGATCAGGTACGTTTGCTGCTAGAATGGCTGAAAAGAATGTGAATATAATCAGTAATACATTGAATATAGATGCTCCTTTCAGCGAATTCATAGCTGCGAGAGGAATTTTTCCGTTGTTCATGAGTTTGGATCAGAGATTACCGTTCTACGACAATGTTTTCGATCTCATACATGCATCTAACGGATTGGATTTAGCGGTTAGTAATAAACCCGAGAAGCTGGAGTTCTTGATGTTTGATCTTGATCGGATCTTGAAACCCGGTGGATTGTTCTGGTTAGACAATTTCTATTGCGGTAACGATGAGAAGAAGAGAGTTCTTACGCGTTTGATAGAGAGGTTTGGGTATAAGAAGTTGAAATGGGTTGTTGGAGAGAAAACTGATGCTGAGGTTTTTCTCTCGGCTGTTCTGCAAAAGCCTGCCCGGATTTGA

Protein Analysis

414

Amino Acids

46.52

Weight (kDa)

8.47

Isoelectric Point (pI)

45.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 259 - 365 2e-07 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012101)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27230 AT5G40830 AT5G40830 AT5G40830
fragaria_vesca FvH4_2g38780
malus_domestica MD08G1015600.v1.1 MD15G1014500.v1.1
prunus_persica Prupe.1G368400_v2.0.a1
pyrus_communis pycom15g01260
rosa_chinensis RchiOBHm_Chr6g0302531
rosa_laevigata RLG00000011165
rosa_multiflora Rmu_sc0008826.1_g000003
rosa_roxburghii Rroxscaffold_7G00165650
rosa_rugosa Rorug06G0314700
rosa_samantha Rh6AG428000 Rh6BG474700 Rh6CG440100 Rh6DG427100
rosa_wichuraiana Rw6G037020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 621
AccII CGCG 1 cut(s) 1138
AciI CCGC 4 cut(s) 36, 621, 1010, 1107
AclWI GGATC 4 cut(s) 13, 823, 951, 1070
AcsI RAATTY 2 cut(s) 899, 918
AcuI CTGAAG 2 cut(s) 38, 51
AfaI GTAC 2 cut(s) 367, 823
AflIII ACRYGT 1 cut(s) 1136
AgsI TTSAA 7 cut(s) 46, 332, 458, 652, 877, 1069, 1171
AhlI ACTAGT 1 cut(s) 701
AjnI CCWGG 1 cut(s) 556
AluBI AGCT 7 cut(s) 71, 316, 343, 485, 698, 908, 1033
AluI AGCT 7 cut(s) 71, 316, 343, 485, 698, 908, 1033
Alw26I GTCTC 6 cut(s) 141, 147, 187, 420, 566, 583
AlwI GGATC 4 cut(s) 13, 823, 951, 1070
Ama87I CYCGRG 1 cut(s) 1025
ApeKI GCWGC 3 cut(s) 630, 830, 908
ApoI RAATTY 2 cut(s) 899, 918
ArsI GACNNNNNNTTYG 2 cut(s) 956, 988
AsuC2I CCSGG 3 cut(s) 671, 1074, 1237
AsuII TTCGAA 1 cut(s) 642
AvaI CYCGRG 1 cut(s) 1025
BbsI GAAGAC 1 cut(s) 746
BbvCI CCTCAGC 1 cut(s) 1200
BbvI GCAGC 3 cut(s) 617, 817, 895
BccI CCATC 3 cut(s) 23, 247, 773
BceAI ACGGC 1 cut(s) 510
BcgI CGANNNNNNTGC 2 cut(s) 622, 656
BciT130I CCWGG 1 cut(s) 558
BcnI CCSGG 3 cut(s) 671, 1074, 1237
BcoDI GTCTC 6 cut(s) 141, 147, 187, 420, 566, 583
BcuI ACTAGT 1 cut(s) 701
BfaI CTAG 5 cut(s) 419, 482, 695, 702, 834
BfmI CTRYAG 1 cut(s) 96
BisI GCNGC 3 cut(s) 631, 831, 909
BlsI GCNGC 3 cut(s) 632, 832, 910
Bme1390I CCNGG 4 cut(s) 558, 671, 1074, 1237
BmeT110I CYCGRG 1 cut(s) 1025
BmiI GGNNCC 2 cut(s) 34, 479
BmrFI CCNGG 4 cut(s) 558, 671, 1074, 1237
BmsI GCATC 4 cut(s) 398, 874, 998, 1186
BpiI GAAGAC 1 cut(s) 746
BplI GAGNNNNNCTC 2 cut(s) 469, 501
BpmI CTGGAG 2 cut(s) 637, 1055
Bpu10I CCTNAGC 1 cut(s) 1200
Bpu14I TTCGAA 1 cut(s) 642
BpuMI CCSGG 3 cut(s) 671, 1074, 1237
Bsa29I ATCGAT 1 cut(s) 225
BsaBI GATNNNNATC 2 cut(s) 10, 1056
BsaI GGTCTC 3 cut(s) 147, 566, 583
Bse1I ACTGG 3 cut(s) 373, 593, 620
Bse8I GATNNNNATC 2 cut(s) 10, 1056
BseBI CCWGG 1 cut(s) 558
BseCI ATCGAT 1 cut(s) 225
BseGI GGATG 1 cut(s) 210
BseJI GATNNNNATC 2 cut(s) 10, 1056
BseMII CTCAG 6 cut(s) 229, 263, 446, 500, 656, 1191
BseNI ACTGG 3 cut(s) 373, 593, 620
BseXI GCAGC 3 cut(s) 617, 817, 895
Bsh1236I CGCG 1 cut(s) 1138
BshVI ATCGAT 1 cut(s) 225
BsiHKCI CYCGRG 1 cut(s) 1025
BsiSI CCGG 3 cut(s) 670, 1074, 1237
BsmAI GTCTC 6 cut(s) 141, 147, 187, 420, 566, 583
BsmBI CGTCTC 1 cut(s) 141
BsmI GAATGC 1 cut(s) 650
Bso31I GGTCTC 3 cut(s) 147, 566, 583
BsoBI CYCGRG 1 cut(s) 1025
Bsp119I TTCGAA 1 cut(s) 642
BspACI CCGC 4 cut(s) 36, 621, 1010, 1107
BspCNI CTCAG 6 cut(s) 228, 262, 445, 499, 657, 1192
BspDI ATCGAT 1 cut(s) 225
BspFNI CGCG 1 cut(s) 1138
BspHI TCATGA 2 cut(s) 706, 933
BspLI GGNNCC 2 cut(s) 34, 479
BspPI GGATC 4 cut(s) 13, 823, 951, 1070
BspT104I TTCGAA 1 cut(s) 642
BspTNI GGTCTC 3 cut(s) 147, 566, 583
BsrBI CCGCTC 1 cut(s) 621
BsrI ACTGG 3 cut(s) 373, 593, 620
Bst2UI CCWGG 1 cut(s) 558
Bst4CI ACNGT 4 cut(s) 516, 547, 605, 957
Bst6I CTCTTC 2 cut(s) 171, 1118
BstBI TTCGAA 1 cut(s) 642
BstC8I GCNNGC 1 cut(s) 1233
BstDEI CTNAG 6 cut(s) 215, 249, 432, 486, 665, 1200
BstF5I GGATG 1 cut(s) 210
BstFNI CGCG 1 cut(s) 1138
BstMAI GTCTC 6 cut(s) 141, 147, 187, 420, 566, 583
BstMWI GCNNNNNNNGC 4 cut(s) 55, 125, 627, 839
BstNI CCWGG 1 cut(s) 558
BstNSI RCATGY 1 cut(s) 989
BstSCI CCNGG 4 cut(s) 556, 669, 1072, 1235
BstSFI CTRYAG 1 cut(s) 96
BstUI CGCG 1 cut(s) 1138
BstV1I GCAGC 3 cut(s) 617, 817, 895
BstV2I GAAGAC 1 cut(s) 746
BstX2I RGATCY 2 cut(s) 5, 1062
BstYI RGATCY 2 cut(s) 5, 1062
Bsu15I ATCGAT 1 cut(s) 225
BsuTUI ATCGAT 1 cut(s) 225
BtgZI GCGATG 2 cut(s) 42, 792
BtsCI GGATG 1 cut(s) 210
BtsI GCAGTG 1 cut(s) 817
BtsIMutI CAGTG 1 cut(s) 817
Cac8I GCNNGC 1 cut(s) 1233
CciI TCATGA 2 cut(s) 706, 933
ClaI ATCGAT 1 cut(s) 225
CsiI ACCWGGT 1 cut(s) 556
Csp6I GTAC 2 cut(s) 366, 822
CviAII CATG 3 cut(s) 707, 934, 986
CviQI GTAC 2 cut(s) 366, 822
DdeI CTNAG 6 cut(s) 215, 249, 432, 486, 665, 1200
Eam1104I CTCTTC 2 cut(s) 171, 1118
EarI CTCTTC 2 cut(s) 171, 1118
Eco31I GGTCTC 3 cut(s) 147, 566, 583
Eco57I CTGAAG 2 cut(s) 38, 51
Eco88I CYCGRG 1 cut(s) 1025
EcoRI GAATTC 1 cut(s) 899
EcoRII CCWGG 1 cut(s) 556
EcoT22I ATGCAT 1 cut(s) 991
Esp3I CGTCTC 1 cut(s) 141
FaeI CATG 3 cut(s) 710, 937, 989
FatI CATG 3 cut(s) 706, 933, 985
Fnu4HI GCNGC 3 cut(s) 631, 831, 909
FokI GGATG 1 cut(s) 197
Fsp4HI GCNGC 3 cut(s) 631, 831, 909
FspBI CTAG 5 cut(s) 419, 482, 695, 702, 834
GluI GCNGC 3 cut(s) 631, 831, 909
GsuI CTGGAG 2 cut(s) 637, 1055
HapII CCGG 3 cut(s) 670, 1074, 1237
Hin1II CATG 3 cut(s) 710, 937, 989
HincII GTYRAC 1 cut(s) 613
HindII GTYRAC 1 cut(s) 613
HinfI GANTC 7 cut(s) 42, 227, 278, 359, 496, 578, 798
HpaI GTTAAC 1 cut(s) 613
HpaII CCGG 3 cut(s) 670, 1074, 1237
Hpy166II GTNNAC 2 cut(s) 512, 613
Hpy188I TCNGA 6 cut(s) 218, 252, 489, 791, 948, 1062
Hpy8I GTNNAC 2 cut(s) 512, 613
Hpy99I CGWCG 1 cut(s) 531
HpyAV CCTTC 2 cut(s) 139, 338
HpyCH4III ACNGT 4 cut(s) 516, 547, 605, 957
HpyCH4IV ACGT 1 cut(s) 824
HpyCH4V TGCA 6 cut(s) 119, 389, 550, 633, 989, 1225
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 125, 627, 839
HpyF3I CTNAG 6 cut(s) 215, 249, 432, 486, 665, 1200
HpySE526I ACGT 1 cut(s) 824
Hsp92II CATG 3 cut(s) 710, 937, 989
KspAI GTTAAC 1 cut(s) 613
LmnI GCTCC 3 cut(s) 477, 618, 892
Lsp1109I GCAGC 3 cut(s) 617, 817, 895
LweI GCATC 4 cut(s) 398, 874, 998, 1186
MabI ACCWGGT 1 cut(s) 556
MaeI CTAG 5 cut(s) 419, 482, 695, 702, 834
MaeII ACGT 1 cut(s) 824
MaeIII GTNAC 3 cut(s) 100, 599, 1109
MbiI CCGCTC 1 cut(s) 621
MboII GAAGA 6 cut(s) 31, 57, 158, 751, 1132, 1135
MflI RGATCY 2 cut(s) 5, 1062
MluCI AATT 3 cut(s) 899, 918, 1096
MluI ACGCGT 1 cut(s) 1136
MmeI TCCRAC 3 cut(s) 198, 376, 1162
MnlI CCTC 8 cut(s) 89, 174, 224, 241, 357, 908, 1143, 1195
Mph1103I ATGCAT 1 cut(s) 991
MseI TTAA 4 cut(s) 318, 612, 726, 732
MslI CAYNNNNRTG 3 cut(s) 251, 380, 405
MspI CCGG 3 cut(s) 670, 1074, 1237
MspR9I CCNGG 4 cut(s) 558, 671, 1074, 1237
Mva1269I GAATGC 1 cut(s) 650
MvaI CCWGG 1 cut(s) 558
MvnI CGCG 1 cut(s) 1138
MwoI GCNNNNNNNGC 4 cut(s) 55, 125, 627, 839
NciI CCSGG 3 cut(s) 671, 1074, 1237
NlaIII CATG 3 cut(s) 710, 937, 989
NlaIV GGNNCC 2 cut(s) 34, 479
NmeAIII GCCGAG 1 cut(s) 1193
NmuCI GTSAC 1 cut(s) 100
NsiI ATGCAT 1 cut(s) 991
NspI RCATGY 1 cut(s) 989
NspV TTCGAA 1 cut(s) 642
PagI TCATGA 2 cut(s) 706, 933
PctI GAATGC 1 cut(s) 650
PfeI GAWTC 7 cut(s) 42, 227, 278, 359, 496, 578, 798
PkrI GCNGC 3 cut(s) 632, 832, 910
Psp6I CCWGG 1 cut(s) 556
PspGI CCWGG 1 cut(s) 556
PspN4I GGNNCC 2 cut(s) 34, 479
PsuI RGATCY 2 cut(s) 5, 1062
RsaI GTAC 2 cut(s) 367, 823
RsaNI GTAC 2 cut(s) 366, 822
RseI CAYNNNNRTG 3 cut(s) 251, 380, 405
SaqAI TTAA 4 cut(s) 318, 612, 726, 732
SatI GCNGC 3 cut(s) 631, 831, 909
ScrFI CCNGG 4 cut(s) 558, 671, 1074, 1237
SexAI ACCWGGT 1 cut(s) 556
SfaNI GCATC 4 cut(s) 398, 874, 998, 1186
SfcI CTRYAG 1 cut(s) 96
SfuI TTCGAA 1 cut(s) 642
SmiMI CAYNNNNRTG 3 cut(s) 251, 380, 405
SpeI ACTAGT 1 cut(s) 701
Sse9I AATT 3 cut(s) 899, 918, 1096
SsiI CCGC 4 cut(s) 36, 621, 1010, 1107
SspMI CTAG 5 cut(s) 419, 482, 695, 702, 834
StyD4I CCNGG 4 cut(s) 556, 669, 1072, 1235
TaaI ACNGT 4 cut(s) 516, 547, 605, 957
TaiI ACGT 1 cut(s) 827
TaqI TCGA 6 cut(s) 225, 285, 529, 642, 801, 975
TaqII GACCGA 1 cut(s) 129
TasI AATT 3 cut(s) 899, 918, 1096
TfiI GAWTC 7 cut(s) 42, 227, 278, 359, 496, 578, 798
Tru1I TTAA 4 cut(s) 318, 612, 726, 732
Tru9I TTAA 4 cut(s) 318, 612, 726, 732
TscAI CASTG 1 cut(s) 817
TseFI GTSAC 1 cut(s) 100
TseI GCWGC 3 cut(s) 630, 830, 908
Tsp45I GTSAC 1 cut(s) 100
TspDTI ATGAA 4 cut(s) 158, 291, 892, 922
TspGWI ACGGA 3 cut(s) 491, 915, 1011
TspRI CASTG 1 cut(s) 817
XapI RAATTY 2 cut(s) 899, 918
XbaI TCTAGA 1 cut(s) 418
XceI RCATGY 1 cut(s) 989
XspI CTAG 5 cut(s) 419, 482, 695, 702, 834
Zsp2I ATGCAT 1 cut(s) 991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.