pycom15g01260

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012101
Physical Location & Seq
Reverse (-)
729263 .. 730528
1266 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g01260.1

Sequence Viewer

Length: 1266 bp
ATGGGCTCCGTTTCTCTCAAGATCGGCGACGGAACCGCCAGATTCCGAAGAGCGTCGCTCTGTTCCTCCGCCGTCAACCTCCTCATGCTCTTCTCTGTCATCACTACCAACCTCTTCGCCCTCTACGCCTTCACATCCGCCCCGAAAGACCACCAAACGTACCACCTCCTCCACCGCACCCAGAAGAACATCTCCCTCATCTCCGAGCAGGTCGGTCTGATCCTCCGCGAGATCGATTCGTCGCAAAAGAAGCTTGCCCAGATGGAAAAGGAGCTCCTTGGATACAAAAGCATCGATCTTTCGAAACCCAATGTCGCGAACGAGCTCAAAGTCTTCCTCCAGCACCACCAGCTCCCTCTCGGAAAAGATTCGAAAACGGGGATCACGGAAATGGTGGCTTCCGTCGGCCATTCCTGCGAGAAATCCGCTGACTTGCTTTCACAGTACATGAACTACAAGGTCTCCGGGCCCTGCCCCGATGATTGGAGCCTTGCCCAGAAGCTGATTCTGCGGGGATGCGAGCCACTGCCGCGACGGAGGTGCTTTGCCAAGACGGTTCCTAAGGTGGGTTTAAACCCTTTACCGATTTCGCTTTGGAAACCTGTTAGTGATAAGATTGTGAGTTGGAGTGGCCTTGGATGCAAGAAATTTGAATGCTTGAATGCTAAGAAATTGAATAGGGATTGCGTTGGTTGTTTCGATTTGATTAACGGGTCCGAAACCCAGAGATATGTTAAGGCTAGGAGCAAAAATGATTTCCTCATTGATGATGTTTTAGCTTTGGGGAGTGGAGGAATCAGAATGGGTTTTGATATTGGAGGTGGGTCTGGTACCTTTGCTGCTAGAATGGCAGAAAGGAACATGACTGTGATCACCAACACATTGAATATCGAAGCCCCGTTTAGCGAATTCATAGCAGCAAGAGGGCTTTTCCCTCTTTTTTTGAGCTTAGATCATAGGTTTCCTTTCTACGATAATGTGTTCGATTTGGTTCATGCCGCTAGTGGATTGGATGTTGCGGGAAAACCGGAAAAGTTTGAGTTCTTGATGTTTGACATCGACCGCATTTTGAGGCCTGGAGGTTTGTTTTGGTTGGACAACTTTTATGGCTCCGATGATGAAAAGAAGAGGGATTTAACCAGATTGATCGAGCGGTTTGGATATAAAAAGCTGAAATGGGTTGTCGGGGAGAAGGCGGGGGCAGCAGGGTCAGGCAAATCAGAGATCTATTTGTCTGCTGTTTTGCAGAAACCAGTTAGAGTATGA

Protein Analysis

422

Amino Acids

46.83

Weight (kDa)

9.23

Isoelectric Point (pI)

36.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 263 - 364 3e-07 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012101)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27230 AT5G40830 AT5G40830 AT5G40830
fragaria_vesca FvH4_2g38780
malus_domestica MD08G1015600.v1.1 MD15G1014500.v1.1
prunus_persica Prupe.1G368400_v2.0.a1
pyrus_communis pycom15g01260
rosa_chinensis RchiOBHm_Chr6g0302531
rosa_laevigata RLG00000011165
rosa_multiflora Rmu_sc0008826.1_g000003
rosa_roxburghii Rroxscaffold_7G00165650
rosa_rugosa Rorug06G0314700
rosa_samantha Rh6AG428000 Rh6BG474700 Rh6CG440100 Rh6DG427100
rosa_wichuraiana Rw6G037020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 199
Acc65I GGTACC 1 cut(s) 830
AccB1I GGYRCC 1 cut(s) 830
AccBSI CCGCTC 1 cut(s) 1153
AccII CGCG 3 cut(s) 228, 317, 532
AclWI GGATC 2 cut(s) 214, 389
AcoI YGGCCR 1 cut(s) 406
AcsI RAATTY 2 cut(s) 647, 908
AfaI GTAC 3 cut(s) 161, 446, 832
AfiI CCNNNNNNNGG 2 cut(s) 483, 566
AgsI TTSAA 4 cut(s) 653, 661, 676, 886
AjnI CCWGG 1 cut(s) 1075
AluBI AGCT 8 cut(s) 253, 274, 325, 352, 502, 779, 948, 1171
AluI AGCT 8 cut(s) 253, 274, 325, 352, 502, 779, 948, 1171
Alw21I GWGCWC 2 cut(s) 276, 327
Alw26I GTCTC 1 cut(s) 466
AlwI GGATC 2 cut(s) 214, 389
AlwNI CAGNNNCTG 1 cut(s) 502
AoxI GGCC 4 cut(s) 406, 467, 631, 1073
ApaI GGGCCC 1 cut(s) 471
ApeKI GCWGC 3 cut(s) 839, 917, 1202
ApoI RAATTY 2 cut(s) 647, 908
Asp700I GAANNNNTTC 1 cut(s) 367
Asp718I GGTACC 1 cut(s) 830
AspS9I GGNCC 3 cut(s) 467, 468, 714
AsuC2I CCSGG 1 cut(s) 466
AsuHPI GGTGA 1 cut(s) 865
AsuII TTCGAA 2 cut(s) 302, 371
AvaII GGWCC 1 cut(s) 714
AxyI CCTNAGG 1 cut(s) 561
BaeGI GKGCMC 1 cut(s) 471
BanI GGYRCC 1 cut(s) 830
BanII GRGCYC 4 cut(s) 8, 276, 327, 471
BbsI GAAGAC 1 cut(s) 325
Bbv12I GWGCWC 2 cut(s) 276, 327
BbvI GCAGC 3 cut(s) 826, 929, 1214
BccI CCATC 1 cut(s) 256
BceAI ACGGC 1 cut(s) 56
BcgI CGANNNNNNTGC 2 cut(s) 522, 556
BciT130I CCWGG 1 cut(s) 1077
BciVI GTATCC 1 cut(s) 275
BclI TGATCA 1 cut(s) 870
BcnI CCSGG 1 cut(s) 466
BcoDI GTCTC 1 cut(s) 466
BfaI CTAG 3 cut(s) 741, 843, 1002
BfuAI ACCTGC 1 cut(s) 199
BfuI GTATCC 1 cut(s) 275
BglII AGATCT 1 cut(s) 1224
BisI GCNGC 5 cut(s) 530, 840, 918, 999, 1203
BlsI GCNGC 5 cut(s) 531, 841, 919, 1000, 1204
Bme1390I CCNGG 2 cut(s) 466, 1077
Bme18I GGWCC 1 cut(s) 714
BmgT120I GGNCC 3 cut(s) 467, 468, 714
BmiI GGNNCC 8 cut(s) 7, 34, 469, 488, 558, 715, 832, 1111
BmrFI CCNGG 2 cut(s) 466, 1077
BmsI GCATC 3 cut(s) 300, 506, 629
BpiI GAAGAC 1 cut(s) 325
BplI GAGNNNNNCTC 2 cut(s) 42, 74
BpmI CTGGAG 2 cut(s) 323, 1098
Bpu14I TTCGAA 2 cut(s) 302, 371
BpuMI CCSGG 1 cut(s) 466
Bsa29I ATCGAT 2 cut(s) 234, 294
BsaI GGTCTC 1 cut(s) 466
BsaJI CCNNGG 2 cut(s) 277, 634
BsaWI WCCGGW 1 cut(s) 1027
BsaXI ACNNNNNCTCC 2 cut(s) 446, 476
Bsc4I CCNNNNNNNGG 2 cut(s) 483, 566
Bse1I ACTGG 1 cut(s) 1253
Bse21I CCTNAGG 1 cut(s) 561
BseBI CCWGG 1 cut(s) 1077
BseCI ATCGAT 2 cut(s) 234, 294
BseDI CCNNGG 2 cut(s) 277, 634
BseGI GGATG 4 cut(s) 134, 521, 644, 1018
BseLI CCNNNNNNNGG 2 cut(s) 483, 566
BseNI ACTGG 1 cut(s) 1253
BseRI GAGGAG 2 cut(s) 71, 158
BseSI GKGCMC 1 cut(s) 471
BseXI GCAGC 3 cut(s) 826, 929, 1214
Bsh1236I CGCG 3 cut(s) 228, 317, 532
Bsh1285I CGRYCG 1 cut(s) 1063
BshFI GGCC 4 cut(s) 408, 469, 633, 1075
BshNI GGYRCC 1 cut(s) 830
BshVI ATCGAT 2 cut(s) 234, 294
BsiEI CGRYCG 1 cut(s) 1063
BsiHKAI GWGCWC 2 cut(s) 276, 327
BsiSI CCGG 2 cut(s) 465, 1028
BslI CCNNNNNNNGG 2 cut(s) 483, 566
BsmAI GTCTC 1 cut(s) 466
BsmI GAATGC 2 cut(s) 659, 667
BsnI GGCC 4 cut(s) 408, 469, 633, 1075
Bso31I GGTCTC 1 cut(s) 466
Bsp119I TTCGAA 2 cut(s) 302, 371
Bsp120I GGGCCC 1 cut(s) 467
Bsp1286I GDGCHC 4 cut(s) 8, 276, 327, 471
Bsp143I GATC 9 cut(s) 21, 219, 231, 295, 381, 870, 952, 1146, 1224
Bsp68I TCGCGA 1 cut(s) 317
BspANI GGCC 4 cut(s) 408, 469, 633, 1075
BspDI ATCGAT 2 cut(s) 234, 294
BspFNI CGCG 3 cut(s) 228, 317, 532
BspLI GGNNCC 8 cut(s) 7, 34, 469, 488, 558, 715, 832, 1111
BspMI ACCTGC 1 cut(s) 199
BspPI GGATC 2 cut(s) 214, 389
BspQI GCTCTTC 2 cut(s) 43, 95
BspT104I TTCGAA 2 cut(s) 302, 371
BspT107I GGYRCC 1 cut(s) 830
BspTNI GGTCTC 1 cut(s) 466
BsrBI CCGCTC 1 cut(s) 1153
BsrI ACTGG 1 cut(s) 1253
BssECI CCNNGG 2 cut(s) 277, 634
BssMI GATC 9 cut(s) 21, 219, 231, 295, 381, 870, 952, 1146, 1224
BssT1I CCWWGG 2 cut(s) 277, 634
Bst2UI CCWGG 1 cut(s) 1077
Bst4CI ACNGT 3 cut(s) 444, 556, 868
Bst6I CTCTTC 4 cut(s) 43, 95, 119, 1121
BstBI TTCGAA 2 cut(s) 302, 371
BstC8I GCNNGC 2 cut(s) 255, 521
BstDEI CTNAG 3 cut(s) 561, 666, 949
BstF5I GGATG 4 cut(s) 134, 521, 644, 1018
BstFNI CGCG 3 cut(s) 228, 317, 532
BstKTI GATC 9 cut(s) 24, 222, 234, 298, 384, 873, 955, 1149, 1227
BstMAI GTCTC 1 cut(s) 466
BstMBI GATC 9 cut(s) 21, 219, 231, 295, 381, 870, 952, 1146, 1224
BstMCI CGRYCG 1 cut(s) 1063
BstMWI GCNNNNNNNGC 9 cut(s) 125, 250, 349, 414, 508, 529, 639, 848, 1202
BstNI CCWGG 1 cut(s) 1077
BstSCI CCNGG 2 cut(s) 464, 1075
BstSLI GKGCMC 1 cut(s) 471
BstUI CGCG 3 cut(s) 228, 317, 532
BstV1I GCAGC 3 cut(s) 826, 929, 1214
BstV2I GAAGAC 1 cut(s) 325
BstX2I RGATCY 1 cut(s) 1224
BstYI RGATCY 1 cut(s) 1224
Bsu15I ATCGAT 2 cut(s) 234, 294
Bsu36I CCTNAGG 1 cut(s) 561
BsuI GTATCC 1 cut(s) 275
BsuRI GGCC 4 cut(s) 408, 469, 633, 1075
BsuTUI ATCGAT 2 cut(s) 234, 294
BtsCI GGATG 4 cut(s) 134, 521, 644, 1018
BtsI GCAGTG 1 cut(s) 524
BtsIMutI CAGTG 1 cut(s) 524
BtuMI TCGCGA 1 cut(s) 317
BveI ACCTGC 1 cut(s) 199
Cac8I GCNNGC 2 cut(s) 255, 521
CaiI CAGNNNCTG 1 cut(s) 502
Cfr13I GGNCC 3 cut(s) 467, 468, 714
ClaI ATCGAT 2 cut(s) 234, 294
CseI GACGC 1 cut(s) 42
Csp6I GTAC 3 cut(s) 160, 445, 831
CviAII CATG 4 cut(s) 85, 448, 862, 995
CviQI GTAC 3 cut(s) 160, 445, 831
DdeI CTNAG 3 cut(s) 561, 666, 949
DpnI GATC 9 cut(s) 23, 221, 233, 297, 383, 872, 954, 1148, 1226
DpnII GATC 9 cut(s) 21, 219, 231, 295, 381, 870, 952, 1146, 1224
DraI TTTAAA 1 cut(s) 573
EaeI YGGCCR 1 cut(s) 406
Eam1104I CTCTTC 4 cut(s) 43, 95, 119, 1121
EarI CTCTTC 4 cut(s) 43, 95, 119, 1121
EciI GGCGGA 2 cut(s) 58, 127
Ecl136II GAGCTC 2 cut(s) 274, 325
Eco130I CCWWGG 2 cut(s) 277, 634
Eco147I AGGCCT 1 cut(s) 1075
Eco24I GRGCYC 4 cut(s) 8, 276, 327, 471
Eco31I GGTCTC 1 cut(s) 466
Eco47I GGWCC 1 cut(s) 714
Eco53kI GAGCTC 2 cut(s) 274, 325
Eco81I CCTNAGG 1 cut(s) 561
EcoICRI GAGCTC 2 cut(s) 274, 325
EcoO109I RGGNCCY 1 cut(s) 468
EcoRI GAATTC 1 cut(s) 908
EcoRII CCWGG 1 cut(s) 1075
EcoT14I CCWWGG 2 cut(s) 277, 634
EcoT38I GRGCYC 4 cut(s) 8, 276, 327, 471
ErhI CCWWGG 2 cut(s) 277, 634
FaeI CATG 4 cut(s) 88, 451, 865, 998
FatI CATG 4 cut(s) 84, 447, 861, 994
FauI CCCGC 3 cut(s) 504, 1012, 1189
FbaI TGATCA 1 cut(s) 870
Fnu4HI GCNGC 5 cut(s) 530, 840, 918, 999, 1203
FokI GGATG 4 cut(s) 121, 528, 651, 1025
FriOI GRGCYC 4 cut(s) 8, 276, 327, 471
Fsp4HI GCNGC 5 cut(s) 530, 840, 918, 999, 1203
FspBI CTAG 3 cut(s) 741, 843, 1002
GluI GCNGC 5 cut(s) 530, 840, 918, 999, 1203
GsuI CTGGAG 2 cut(s) 323, 1098
HaeIII GGCC 4 cut(s) 408, 469, 633, 1075
HapII CCGG 2 cut(s) 465, 1028
HgaI GACGC 1 cut(s) 42
Hin1II CATG 4 cut(s) 88, 451, 865, 998
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HindIII AAGCTT 1 cut(s) 251
HinfI GANTC 5 cut(s) 42, 236, 368, 505, 795
HpaII CCGG 2 cut(s) 465, 1028
HphI GGTGA 1 cut(s) 865
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 8 cut(s) 47, 205, 219, 362, 718, 800, 1114, 1222
Hpy188III TCNNGA 3 cut(s) 19, 316, 1045
Hpy8I GTNNAC 1 cut(s) 76
Hpy99I CGWCG 5 cut(s) 32, 58, 244, 407, 537
HpyAV CCTTC 2 cut(s) 139, 1186
HpyCH4III ACNGT 3 cut(s) 444, 556, 868
HpyCH4IV ACGT 1 cut(s) 158
HpyCH4V TGCA 2 cut(s) 642, 1246
HpyF10VI GCNNNNNNNGC 9 cut(s) 125, 250, 349, 414, 508, 529, 639, 848, 1202
HpyF3I CTNAG 3 cut(s) 561, 666, 949
HpySE526I ACGT 1 cut(s) 158
Hsp92II CATG 4 cut(s) 88, 451, 865, 998
KpnI GGTACC 1 cut(s) 834
Ksp22I TGATCA 1 cut(s) 870
Kzo9I GATC 9 cut(s) 21, 219, 231, 295, 381, 870, 952, 1146, 1224
LguI GCTCTTC 2 cut(s) 43, 95
LmnI GCTCC 7 cut(s) 11, 271, 279, 357, 486, 744, 1115
Lsp1109I GCAGC 3 cut(s) 826, 929, 1214
LweI GCATC 3 cut(s) 300, 506, 629
MaeI CTAG 3 cut(s) 741, 843, 1002
MaeII ACGT 1 cut(s) 158
MalI GATC 9 cut(s) 23, 221, 233, 297, 383, 872, 954, 1148, 1226
MbiI CCGCTC 1 cut(s) 1153
MboI GATC 9 cut(s) 21, 219, 231, 295, 381, 870, 952, 1146, 1224
MboII GAAGA 6 cut(s) 60, 82, 106, 196, 325, 1138
MflI RGATCY 1 cut(s) 1224
MhlI GDGCHC 4 cut(s) 8, 276, 327, 471
MluCI AATT 3 cut(s) 647, 671, 908
MmeI TCCRAC 2 cut(s) 605, 1074
MroXI GAANNNNTTC 1 cut(s) 367
MseI TTAA 4 cut(s) 572, 708, 735, 1136
MslI CAYNNNNRTG 2 cut(s) 389, 866
MspA1I CMGCKG 1 cut(s) 428
MspI CCGG 2 cut(s) 465, 1028
MspR9I CCNGG 2 cut(s) 466, 1077
MssI GTTTAAAC 1 cut(s) 573
Mva1269I GAATGC 2 cut(s) 659, 667
MvaI CCWGG 1 cut(s) 1077
MvnI CGCG 3 cut(s) 228, 317, 532
MwoI GCNNNNNNNGC 9 cut(s) 125, 250, 349, 414, 508, 529, 639, 848, 1202
NciI CCSGG 1 cut(s) 466
NdeII GATC 9 cut(s) 21, 219, 231, 295, 381, 870, 952, 1146, 1224
NlaIII CATG 4 cut(s) 88, 451, 865, 998
NlaIV GGNNCC 8 cut(s) 7, 34, 469, 488, 558, 715, 832, 1111
NruI TCGCGA 1 cut(s) 317
NspV TTCGAA 2 cut(s) 302, 371
PceI AGGCCT 1 cut(s) 1075
PciSI GCTCTTC 2 cut(s) 43, 95
PctI GAATGC 2 cut(s) 659, 667
PdmI GAANNNNTTC 1 cut(s) 367
PfeI GAWTC 5 cut(s) 42, 236, 368, 505, 795
PkrI GCNGC 5 cut(s) 531, 841, 919, 1000, 1204
PmeI GTTTAAAC 1 cut(s) 573
Psp124BI GAGCTC 2 cut(s) 276, 327
Psp6I CCWGG 1 cut(s) 1075
PspGI CCWGG 1 cut(s) 1075
PspN4I GGNNCC 8 cut(s) 7, 34, 469, 488, 558, 715, 832, 1111
PspOMI GGGCCC 1 cut(s) 467
PspPI GGNCC 3 cut(s) 467, 468, 714
PstNI CAGNNNCTG 1 cut(s) 502
PsuI RGATCY 1 cut(s) 1224
RruI TCGCGA 1 cut(s) 317
RsaI GTAC 3 cut(s) 161, 446, 832
RsaNI GTAC 3 cut(s) 160, 445, 831
RseI CAYNNNNRTG 2 cut(s) 389, 866
SacI GAGCTC 2 cut(s) 276, 327
SapI GCTCTTC 2 cut(s) 43, 95
SaqAI TTAA 4 cut(s) 572, 708, 735, 1136
SatI GCNGC 5 cut(s) 530, 840, 918, 999, 1203
Sau3AI GATC 9 cut(s) 21, 219, 231, 295, 381, 870, 952, 1146, 1224
Sau96I GGNCC 3 cut(s) 467, 468, 714
ScrFI CCNGG 2 cut(s) 466, 1077
SduI GDGCHC 4 cut(s) 8, 276, 327, 471
SfaNI GCATC 3 cut(s) 300, 506, 629
SfuI TTCGAA 2 cut(s) 302, 371
SinI GGWCC 1 cut(s) 714
SmiMI CAYNNNNRTG 2 cut(s) 389, 866
SmlI CTYRAG 1 cut(s) 17
SmoI CTYRAG 1 cut(s) 17
Sse9I AATT 3 cut(s) 647, 671, 908
SseBI AGGCCT 1 cut(s) 1075
SspMI CTAG 3 cut(s) 741, 843, 1002
SstI GAGCTC 2 cut(s) 276, 327
StuI AGGCCT 1 cut(s) 1075
StyD4I CCNGG 2 cut(s) 464, 1075
StyI CCWWGG 2 cut(s) 277, 634
TaaI ACNGT 3 cut(s) 444, 556, 868
TaiI ACGT 1 cut(s) 161
TaqI TCGA 9 cut(s) 234, 294, 302, 371, 699, 891, 984, 1059, 1149
TaqII GACCGA 1 cut(s) 203
TasI AATT 3 cut(s) 647, 671, 908
TatI WGTACW 1 cut(s) 444
TauI GCSGC 2 cut(s) 532, 1001
TfiI GAWTC 5 cut(s) 42, 236, 368, 505, 795
Tru1I TTAA 4 cut(s) 572, 708, 735, 1136
Tru9I TTAA 4 cut(s) 572, 708, 735, 1136
TscAI CASTG 1 cut(s) 531
TseI GCWGC 3 cut(s) 839, 917, 1202
TspDTI ATGAA 4 cut(s) 464, 901, 983, 1134
TspGWI ACGGA 4 cut(s) 45, 391, 401, 550
TspRI CASTG 1 cut(s) 531
VpaK11BI GGWCC 1 cut(s) 714
XapI RAATTY 2 cut(s) 647, 908
XmnI GAANNNNTTC 1 cut(s) 367
XspI CTAG 3 cut(s) 741, 843, 1002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.