AT3G27550

CRS1_YhbY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0011191
Physical Location & Seq
Reverse (-)
10207727 .. 10210231
2505 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G27550.1

Sequence Viewer

Length: 1476 bp
ATGAATCAAGTGTTTAAAGGATGGTCTCGAGGTATGTCAACATCGAGAGGAAGGAGTATGAGGAGCAAAGTTGAGAGTAGAATGCGTAAAGAGTCTGGTAAGACGTTAAGAGAGATTAGGAGAGCGAAGAAGTTGAAGAAGAAACTCATGACTGATGAAGAAAGGCTCATTTACAATCTCAAAAGGGCGAAGAAGAAAGTTGCGCTTTTGTTGCAAAAGCTCAAGAAGTATGATCTTCCTGAGCTGCCATCTCCGGTGCATGATCCCGAGCTTTTCACATCCGAGCAGGTTCAAGCGTTCAAGAAAATCGGTTTCAAGAATAAAAACTATGTTCCTGTTGGTGTTCGTGGAGTCTTTGGAGGAGTGGTACAAAATATGCATATGCACTGGAAGTTTCATGAGACTGTGCAAGTTTGTTGTGATAACTTCCCTAAGGAAAAGATTAAAGAGATGGCGAGCATGATAGCGAGACTGAGTGGCGGGGTAGTCATTAACATACATAATGTAAAAACTATTATTATGTTCCGTGGTAGAAACTACCGGCAGCCCAAGAACCTAATCCCTGTCAACACCCTTACCAAACGGAAGGCTTTATTCAAAGCAAGATTCGAACAAGCGCTTGAATCTCAGAAGCTGAACATAAAGAAAACAGAACAGCAGCTACGGAGAATGGGTGTTAACCCTGAAGATCCGGTTGCAATGGCTAGCATCCACAGAGTGGCCTCGACATTTTTCAATGCAATCGACAAAAAGGAAGGAACACCGTATGTCTTCCATGGAGATAAACAATCAGAACGAGGCACTAGTGTTGACAATAGAGAAGAATCTGAACCGGGCGATGAAGATAGTGACCAGGAGGAGCTAGACAGATTCATAGCTGAGATAGAAGAAGCAGCAGACAAGGAGTGGGAGGAAGAGGAAGCTGCAGAGCAAGAAGAATCTGGTAGGATAAGGTATTGGAACCGAGAAGAGTTTGCAGGAAGAAGCAGAACTCCTGAATTGCGTAGTTATGGAGATGCAAGTCATGGCTTTAGAAGAAATGATAGGGATACACATAGCCAGAGGAGACCCAACGATAGTGATGATGATGACGATGACGGTGAATTGGATTCTGAGGATGATGAAATTCCAAAGAGATTTGATAGAGCAAGATCATCAAACACAAACACCAGAAGGCCAAGAGAGGATTTTAAGAGAAGAAGCCCCGATCCTAGACCTCGACCTCGAGCTCAAGTGAGGAGTGATGACGATGTATTGAGTGATCTTGATAACACAATGTGGGATTCAGAAGATGAAGAAGATGCACCACCGGCTAATTATATTTCGAGTAGTGATGATGACGAGGATGAAAACAGAACAGTATCAGCATCATCATCTAAACAATCGAGATTCAGGAACAATAGTTCAAGAGATGGTATCAACAATTCAAAGAGTAAGAGTGGAAAACAGAGGGATGAGGATTGGGATAGTGATTAG

Protein Analysis

491

Amino Acids

57.0

Weight (kDa)

7.72

Isoelectric Point (pI)

65.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 93 - 176 2e-14 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011191)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27550 AT3G27550 AT3G27550
fragaria_vesca FvH4_6g51690
malus_domestica MD09G1023500.v1.1 MD17G1023000.v1.1
prunus_persica Prupe.3G293300_v2.0.a1
pyrus_communis pycom111g01960 pycom17g01970
rosa_chinensis RchiOBHm_Chr2g0173221
rosa_laevigata RLG00000022171
rosa_multiflora Rmu_sc0000860.1_g000003 Rmu_sc0039142.1_g000001
rosa_roxburghii Rroxscaffold_2G00079110
rosa_rugosa Rorug02G0566600
rosa_samantha Rh2AG646500 Rh2BG657200 Rh2CG622400 Rh2DG670800
rosa_wichuraiana Rw2G053100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 277
AccB7I CCANNNNNTGG 1 cut(s) 718
AciI CCGC 1 cut(s) 480
AclWI GGATC 3 cut(s) 257, 683, 1202
AcsI RAATTY 1 cut(s) 1125
AcuI CTGAAG 1 cut(s) 705
AdeI CACNNNGTG 2 cut(s) 718, 1278
AfaI GTAC 1 cut(s) 369
AfeI AGCGCT 1 cut(s) 618
AfiI CCNNNNNNNGG 1 cut(s) 718
AgsI TTSAA 9 cut(s) 136, 293, 301, 316, 598, 623, 736, 1407, 1428
AhlI ACTAGT 1 cut(s) 803
AjnI CCWGG 1 cut(s) 852
AloI GAACNNNNNNTCC 2 cut(s) 1387, 1419
AluBI AGCT 9 cut(s) 220, 244, 271, 634, 661, 862, 878, 923, 1229
AluI AGCT 9 cut(s) 220, 244, 271, 634, 661, 862, 878, 923, 1229
Alw21I GWGCWC 1 cut(s) 1231
Alw26I GTCTC 4 cut(s) 30, 395, 463, 1060
AlwI GGATC 3 cut(s) 257, 683, 1202
AlwNI CAGNNNCTG 1 cut(s) 634
Ama87I CYCGRG 3 cut(s) 27, 266, 1224
Aor51HI AGCGCT 1 cut(s) 618
AoxI GGCC 2 cut(s) 720, 1175
ApeKI GCWGC 5 cut(s) 244, 544, 658, 893, 923
ApoI RAATTY 1 cut(s) 1125
AspLEI GCGC 2 cut(s) 205, 619
AsuC2I CCSGG 1 cut(s) 834
AsuHPI GGTGA 1 cut(s) 1112
AsuII TTCGAA 1 cut(s) 609
AsuNHI GCTAGC 1 cut(s) 704
AvaI CYCGRG 3 cut(s) 27, 266, 1224
AxyI CCTNAGG 1 cut(s) 432
BanII GRGCYC 1 cut(s) 1231
BbsI GAAGAC 1 cut(s) 763
Bbv12I GWGCWC 1 cut(s) 1231
BbvI GCAGC 5 cut(s) 231, 556, 670, 905, 910
BccI CCATC 4 cut(s) 15, 256, 445, 1406
BciT130I CCWGG 1 cut(s) 854
BciVI GTATCC 1 cut(s) 1042
BcnI CCSGG 1 cut(s) 834
BcoDI GTCTC 4 cut(s) 30, 395, 463, 1060
BcuI ACTAGT 1 cut(s) 803
BfaI CTAG 4 cut(s) 705, 804, 863, 1212
BfmI CTRYAG 1 cut(s) 924
BfoI RGCGCY 1 cut(s) 620
BfuAI ACCTGC 1 cut(s) 277
BfuI GTATCC 1 cut(s) 1042
BisI GCNGC 5 cut(s) 245, 545, 659, 894, 924
BlsI GCNGC 5 cut(s) 246, 546, 660, 895, 925
Bme1390I CCNGG 2 cut(s) 834, 854
BmeT110I CYCGRG 3 cut(s) 27, 266, 1224
BmiI GGNNCC 1 cut(s) 962
BmrFI CCNGG 2 cut(s) 834, 854
BmsI GCATC 4 cut(s) 717, 1006, 1291, 1376
BmtI GCTAGC 1 cut(s) 708
BpiI GAAGAC 1 cut(s) 763
Bpu10I CCTNAGC 1 cut(s) 240
Bpu14I TTCGAA 1 cut(s) 609
BpuEI CTTGAG 2 cut(s) 206, 1215
BpuMI CCSGG 1 cut(s) 834
BsaI GGTCTC 2 cut(s) 30, 1060
BsaJI CCNNGG 2 cut(s) 526, 775
BsaWI WCCGGW 2 cut(s) 253, 691
BsaXI ACNNNNNCTCC 4 cut(s) 351, 381, 658, 688
Bsc4I CCNNNNNNNGG 1 cut(s) 718
Bse118I RCCGGY 2 cut(s) 540, 1309
Bse1I ACTGG 1 cut(s) 392
Bse21I CCTNAGG 1 cut(s) 432
Bse3DI GCAATG 1 cut(s) 705
BseBI CCWGG 1 cut(s) 854
BseDI CCNNGG 2 cut(s) 526, 775
BseGI GGATG 6 cut(s) 26, 278, 708, 1123, 1351, 1459
BseLI CCNNNNNNNGG 1 cut(s) 718
BseMI GCAATG 1 cut(s) 705
BseMII CTCAG 5 cut(s) 231, 464, 641, 870, 1104
BseNI ACTGG 1 cut(s) 392
BseRI GAGGAG 5 cut(s) 76, 375, 872, 1078, 1252
BseXI GCAGC 5 cut(s) 231, 556, 670, 905, 910
BshFI GGCC 2 cut(s) 722, 1177
BsiHKAI GWGCWC 1 cut(s) 1231
BsiHKCI CYCGRG 3 cut(s) 27, 266, 1224
BsiSI CCGG 5 cut(s) 254, 541, 692, 833, 1310
BslI CCNNNNNNNGG 1 cut(s) 718
BsmAI GTCTC 4 cut(s) 30, 395, 463, 1060
BsmI GAATGC 1 cut(s) 87
BsnI GGCC 2 cut(s) 722, 1177
Bso31I GGTCTC 2 cut(s) 30, 1060
BsoBI CYCGRG 3 cut(s) 27, 266, 1224
Bsp119I TTCGAA 1 cut(s) 609
Bsp1286I GDGCHC 1 cut(s) 1231
Bsp143I GATC 6 cut(s) 232, 262, 688, 1151, 1207, 1261
Bsp19I CCATGG 1 cut(s) 775
BspACI CCGC 1 cut(s) 480
BspANI GGCC 2 cut(s) 722, 1177
BspCNI CTCAG 5 cut(s) 232, 465, 640, 871, 1105
BspHI TCATGA 2 cut(s) 147, 397
BspLI GGNNCC 1 cut(s) 962
BspMAI CTGCAG 1 cut(s) 928
BspMI ACCTGC 1 cut(s) 277
BspOI GCTAGC 1 cut(s) 708
BspPI GGATC 3 cut(s) 257, 683, 1202
BspT104I TTCGAA 1 cut(s) 609
BspTNI GGTCTC 2 cut(s) 30, 1060
BsrDI GCAATG 1 cut(s) 705
BsrFI RCCGGY 2 cut(s) 540, 1309
BsrI ACTGG 1 cut(s) 392
BssAI RCCGGY 2 cut(s) 540, 1309
BssECI CCNNGG 2 cut(s) 526, 775
BssMI GATC 6 cut(s) 232, 262, 688, 1151, 1207, 1261
BssT1I CCWWGG 1 cut(s) 775
Bst2UI CCWGG 1 cut(s) 854
Bst4CI ACNGT 4 cut(s) 406, 765, 1100, 1360
Bst6I CTCTTC 2 cut(s) 909, 963
BstBI TTCGAA 1 cut(s) 609
BstC8I GCNNGC 2 cut(s) 457, 706
BstDEI CTNAG 6 cut(s) 240, 432, 473, 627, 879, 1113
BstDSI CCRYGG 2 cut(s) 526, 775
BstF5I GGATG 6 cut(s) 26, 278, 708, 1123, 1351, 1459
BstH2I RGCGCY 1 cut(s) 620
BstHHI GCGC 2 cut(s) 205, 619
BstKTI GATC 6 cut(s) 235, 265, 691, 1154, 1210, 1264
BstMAI GTCTC 4 cut(s) 30, 395, 463, 1060
BstMBI GATC 6 cut(s) 232, 262, 688, 1151, 1207, 1261
BstMWI GCNNNNNNNGC 2 cut(s) 211, 1310
BstNI CCWGG 1 cut(s) 854
BstSCI CCNGG 2 cut(s) 832, 852
BstSFI CTRYAG 1 cut(s) 924
BstV1I GCAGC 5 cut(s) 231, 556, 670, 905, 910
BstV2I GAAGAC 1 cut(s) 763
BstX2I RGATCY 1 cut(s) 688
BstYI RGATCY 1 cut(s) 688
Bsu36I CCTNAGG 1 cut(s) 432
BsuI GTATCC 1 cut(s) 1042
BsuRI GGCC 2 cut(s) 722, 1177
BtgI CCRYGG 2 cut(s) 526, 775
BtgZI GCGATG 1 cut(s) 852
BtsCI GGATG 6 cut(s) 26, 278, 708, 1123, 1351, 1459
BtsIMutI CAGTG 1 cut(s) 385
BveI ACCTGC 1 cut(s) 277
Cac8I GCNNGC 2 cut(s) 457, 706
CaiI CAGNNNCTG 1 cut(s) 634
CciI TCATGA 2 cut(s) 147, 397
CfoI GCGC 2 cut(s) 205, 619
Cfr10I RCCGGY 2 cut(s) 540, 1309
Csp6I GTAC 1 cut(s) 368
CviAII CATG 6 cut(s) 148, 260, 398, 460, 776, 1025
CviQI GTAC 1 cut(s) 368
DdeI CTNAG 6 cut(s) 240, 432, 473, 627, 879, 1113
DpnI GATC 6 cut(s) 234, 264, 690, 1153, 1209, 1263
DpnII GATC 6 cut(s) 232, 262, 688, 1151, 1207, 1261
DraI TTTAAA 1 cut(s) 16
DraIII CACNNNGTG 2 cut(s) 718, 1278
Eam1104I CTCTTC 2 cut(s) 909, 963
EarI CTCTTC 2 cut(s) 909, 963
Ecl136II GAGCTC 1 cut(s) 1229
Eco130I CCWWGG 1 cut(s) 775
Eco24I GRGCYC 1 cut(s) 1231
Eco31I GGTCTC 2 cut(s) 30, 1060
Eco47III AGCGCT 1 cut(s) 618
Eco53kI GAGCTC 1 cut(s) 1229
Eco57I CTGAAG 1 cut(s) 705
Eco81I CCTNAGG 1 cut(s) 432
Eco88I CYCGRG 3 cut(s) 27, 266, 1224
EcoICRI GAGCTC 1 cut(s) 1229
EcoRII CCWGG 1 cut(s) 852
EcoT14I CCWWGG 1 cut(s) 775
EcoT22I ATGCAT 1 cut(s) 381
EcoT38I GRGCYC 1 cut(s) 1231
ErhI CCWWGG 1 cut(s) 775
FaeI CATG 6 cut(s) 151, 263, 401, 463, 779, 1028
FalI AAGNNNNNCTT 2 cut(s) 189, 221
FatI CATG 6 cut(s) 147, 259, 397, 459, 775, 1024
FauI CCCGC 1 cut(s) 473
FauNDI CATATG 1 cut(s) 381
Fnu4HI GCNGC 5 cut(s) 245, 545, 659, 894, 924
FokI GGATG 6 cut(s) 33, 265, 695, 1130, 1358, 1466
FriOI GRGCYC 1 cut(s) 1231
Fsp4HI GCNGC 5 cut(s) 245, 545, 659, 894, 924
FspBI CTAG 4 cut(s) 705, 804, 863, 1212
GlaI GCGC 2 cut(s) 204, 618
GluI GCNGC 5 cut(s) 245, 545, 659, 894, 924
HaeII RGCGCY 1 cut(s) 620
HaeIII GGCC 2 cut(s) 722, 1177
HapII CCGG 5 cut(s) 254, 541, 692, 833, 1310
HhaI GCGC 2 cut(s) 205, 619
Hin1II CATG 6 cut(s) 151, 263, 401, 463, 779, 1028
Hin6I GCGC 2 cut(s) 203, 617
HinP1I GCGC 2 cut(s) 203, 617
HincII GTYRAC 4 cut(s) 39, 568, 679, 811
HindII GTYRAC 4 cut(s) 39, 568, 679, 811
HpaI GTTAAC 1 cut(s) 679
HpaII CCGG 5 cut(s) 254, 541, 692, 833, 1310
HphI GGTGA 1 cut(s) 1112
Hpy166II GTNNAC 4 cut(s) 39, 568, 679, 811
Hpy188I TCNGA 6 cut(s) 283, 630, 793, 829, 1114, 1288
Hpy8I GTNNAC 4 cut(s) 39, 568, 679, 811
HpyAV CCTTC 4 cut(s) 45, 580, 749, 1167
HpyCH4III ACNGT 4 cut(s) 406, 765, 1100, 1360
HpyCH4IV ACGT 1 cut(s) 104
HpyF10VI GCNNNNNNNGC 2 cut(s) 211, 1310
HpyF3I CTNAG 6 cut(s) 240, 432, 473, 627, 879, 1113
HpySE526I ACGT 1 cut(s) 104
Hsp92II CATG 6 cut(s) 151, 263, 401, 463, 779, 1028
HspAI GCGC 2 cut(s) 203, 617
KspAI GTTAAC 1 cut(s) 679
Kzo9I GATC 6 cut(s) 232, 262, 688, 1151, 1207, 1261
LmnI GCTCC 2 cut(s) 63, 859
Lsp1109I GCAGC 5 cut(s) 231, 556, 670, 905, 910
LweI GCATC 4 cut(s) 717, 1006, 1291, 1376
MaeI CTAG 4 cut(s) 705, 804, 863, 1212
MaeII ACGT 1 cut(s) 104
MaeIII GTNAC 1 cut(s) 848
MalI GATC 6 cut(s) 234, 264, 690, 1153, 1209, 1263
MboI GATC 6 cut(s) 232, 262, 688, 1151, 1207, 1261
MflI RGATCY 1 cut(s) 688
MhlI GDGCHC 1 cut(s) 1231
MluCI AATT 5 cut(s) 998, 1103, 1125, 1315, 1423
MlyI GAGTC 2 cut(s) 101, 360
Mph1103I ATGCAT 1 cut(s) 381
MseI TTAA 6 cut(s) 15, 107, 444, 492, 678, 1191
MspI CCGG 5 cut(s) 254, 541, 692, 833, 1310
MspR9I CCNGG 2 cut(s) 834, 854
Mva1269I GAATGC 1 cut(s) 87
MvaI CCWGG 1 cut(s) 854
MwoI GCNNNNNNNGC 2 cut(s) 211, 1310
NciI CCSGG 1 cut(s) 834
NcoI CCATGG 1 cut(s) 775
NdeI CATATG 1 cut(s) 381
NdeII GATC 6 cut(s) 232, 262, 688, 1151, 1207, 1261
NheI GCTAGC 1 cut(s) 704
NlaIII CATG 6 cut(s) 151, 263, 401, 463, 779, 1028
NlaIV GGNNCC 1 cut(s) 962
NmuCI GTSAC 1 cut(s) 848
NsiI ATGCAT 1 cut(s) 381
NspV TTCGAA 1 cut(s) 609
PaeR7I CTCGAG 2 cut(s) 27, 1224
PagI TCATGA 2 cut(s) 147, 397
PctI GAATGC 1 cut(s) 87
PfeI GAWTC 9 cut(s) 4, 606, 623, 824, 870, 938, 1109, 1283, 1389
PflMI CCANNNNNTGG 1 cut(s) 718
PkrI GCNGC 5 cut(s) 246, 546, 660, 895, 925
PleI GAGTC 2 cut(s) 100, 359
PpsI GAGTC 2 cut(s) 100, 359
Psp124BI GAGCTC 1 cut(s) 1231
Psp6I CCWGG 1 cut(s) 852
PspGI CCWGG 1 cut(s) 852
PspN4I GGNNCC 1 cut(s) 962
PspXI VCTCGAGB 1 cut(s) 1224
PsrI GAACNNNNNNTAC 2 cut(s) 645, 677
PstI CTGCAG 1 cut(s) 928
PstNI CAGNNNCTG 1 cut(s) 634
PsuI RGATCY 1 cut(s) 688
RsaI GTAC 1 cut(s) 369
RsaNI GTAC 1 cut(s) 368
SacI GAGCTC 1 cut(s) 1231
SaqAI TTAA 6 cut(s) 15, 107, 444, 492, 678, 1191
SatI GCNGC 5 cut(s) 245, 545, 659, 894, 924
Sau3AI GATC 6 cut(s) 232, 262, 688, 1151, 1207, 1261
SchI GAGTC 2 cut(s) 101, 360
ScrFI CCNGG 2 cut(s) 834, 854
SduI GDGCHC 1 cut(s) 1231
SfaNI GCATC 4 cut(s) 717, 1006, 1291, 1376
SfcI CTRYAG 1 cut(s) 924
Sfr274I CTCGAG 2 cut(s) 27, 1224
SfuI TTCGAA 1 cut(s) 609
SlaI CTCGAG 2 cut(s) 27, 1224
SmlI CTYRAG 4 cut(s) 27, 221, 1224, 1230
SmoI CTYRAG 4 cut(s) 27, 221, 1224, 1230
SpeI ACTAGT 1 cut(s) 803
Sse9I AATT 5 cut(s) 998, 1103, 1125, 1315, 1423
SsiI CCGC 1 cut(s) 480
SspMI CTAG 4 cut(s) 705, 804, 863, 1212
SstI GAGCTC 1 cut(s) 1231
StyD4I CCNGG 2 cut(s) 832, 852
StyI CCWWGG 1 cut(s) 775
TaaI ACNGT 4 cut(s) 406, 765, 1100, 1360
TaiI ACGT 1 cut(s) 107
TaqI TCGA 9 cut(s) 28, 44, 609, 725, 744, 1219, 1225, 1325, 1385
TasI AATT 5 cut(s) 998, 1103, 1125, 1315, 1423
TfiI GAWTC 9 cut(s) 4, 606, 623, 824, 870, 938, 1109, 1283, 1389
Tru1I TTAA 6 cut(s) 15, 107, 444, 492, 678, 1191
Tru9I TTAA 6 cut(s) 15, 107, 444, 492, 678, 1191
TscAI CASTG 1 cut(s) 392
TseFI GTSAC 1 cut(s) 848
TseI GCWGC 5 cut(s) 244, 544, 658, 893, 923
Tsp45I GTSAC 1 cut(s) 848
TspDTI ATGAA 8 cut(s) 17, 171, 386, 855, 862, 1137, 1308, 1362
TspGWI ACGGA 3 cut(s) 515, 598, 679
TspRI CASTG 1 cut(s) 392
Van91I CCANNNNNTGG 1 cut(s) 718
XapI RAATTY 1 cut(s) 1125
XhoI CTCGAG 2 cut(s) 27, 1224
XspI CTAG 4 cut(s) 705, 804, 863, 1212
Zsp2I ATGCAT 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.