AT3G27950

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0010447
Physical Location & Seq
Forward (+)
10377957 .. 10379907
1951 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G27950.1

Sequence Viewer

Length: 1128 bp
ATGGCTATTTCTAAAATAACCCTTGCAATCATCGTTTTACTTCTAGGGTTCACTGAGAAATTATCTGCCTTGTCGAGTAGTTGTAATTTTCCGGCAGTTTTTAACTTTGGAGATTCAAATTCTGACACCGGAGCTATCTCTGCGGCCATTGGAGAGGTTCCTCCACCTAATGGAGTTGCATTTTTTGGAAGATCCGCCGGAAGACACTCCGATGGCCGTCTCATTATAGATTTCATTACCGAAAATCTTACTTTGCCGTATTTAACACCATACTTGGATTCGGTTGGAGCTAATTACCGGCATGGTGCTAATTTTGCGACGGGCGGTTCTTGCATCCGCCCCACACTTGCTTGTTTTAGCCCATTCCATCTTGGGACTCAAGTGTCCCAATTCATTCACTTTAAGACTCGTACCTTGTCTCTCTACAACCAAACCAACGGAAAAACTCCATTCTGCAAAGAAGTCTTTGCACGGCCCAAAGATTTCTCAAAAGCTCTTTATACATTGGATATCGGCCAAAACGATCTCGCGATTGGGTTCCAAAACATGACAGAGGAACAACTCAAAGCAACTATACCCTTAATCATTGAAAACTTCACCATTGCCTTAAAATTGTTATACAAAGAAGGAGCAAGATTCTTCTCAATTCATAACACCGGACCGACAGGCTGTTTACCGTATCTTCTGAAAGCTTTTCCCGCTATACCGCGAGATCCCTACGGTTGTTTGAAGCCTCTGAACAATGTGGCAATTGAGTTCAACAAACAACTCAAGAACAAAATCACTCAACTAAAGAAAGAGTTACCTTCTTCTTTCTTTACTTATGTTGATGTCTACTCGGCCAAATACAACTTGATCACCAAAGCAAAGGCTCTAGGTTTTATTGATCCTTTTGACTATTGTTGCGTTGGGGCGATCGGACGTGGAATGGGATGTGGGAAGACAATATTTCTAAATGGGACAGAACTCTATAGTTCTTCCTGCCAAAACCGTAAAAATTTCATAAGTTGGGATGGCATACATTACACCGAAACCGCGAATATGCTAGTCGCAAACCGAATCCTCGATGGTTCGATATCGGATCCACCTCTCCCTACCCAAAAAGCATGTAAACTCACGAAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

375

Amino Acids

41.16

Weight (kDa)

9.06

Isoelectric Point (pI)

28.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 33 - 354 2.6e-39 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 382
AccB7I CCANNNNNTGG 1 cut(s) 170
AccI GTMKAC 1 cut(s) 834
AccII CGCG 3 cut(s) 530, 709, 1037
AciI CCGC 7 cut(s) 143, 195, 324, 337, 699, 707, 1035
AclWI GGATC 5 cut(s) 186, 707, 881, 1076, 1089
AcoI YGGCCR 4 cut(s) 144, 214, 514, 840
AcsI RAATTY 2 cut(s) 118, 997
AfaI GTAC 1 cut(s) 412
AfiI CCNNNNNNNGG 1 cut(s) 170
AgsI TTSAA 4 cut(s) 117, 590, 730, 760
AjiI CACGTC 1 cut(s) 923
AluBI AGCT 4 cut(s) 134, 290, 494, 692
AluI AGCT 4 cut(s) 134, 290, 494, 692
Alw26I GTCTC 2 cut(s) 224, 423
AlwI GGATC 5 cut(s) 186, 707, 881, 1076, 1089
AoxI GGCC 5 cut(s) 144, 214, 473, 514, 840
ApoI RAATTY 2 cut(s) 118, 997
AspS9I GGNCC 2 cut(s) 474, 659
AsuHPI GGTGA 2 cut(s) 589, 850
AvaII GGWCC 1 cut(s) 659
BamHI GGATCC 1 cut(s) 1081
BbsI GAAGAC 2 cut(s) 208, 947
BccI CCATC 4 cut(s) 206, 375, 1007, 1061
BceAI ACGGC 3 cut(s) 201, 241, 488
BclI TGATCA 1 cut(s) 855
BcoDI GTCTC 2 cut(s) 224, 423
BfaI CTAG 3 cut(s) 44, 875, 1046
BfmI CTRYAG 1 cut(s) 970
BisI GCNGC 1 cut(s) 144
BlsI GCNGC 1 cut(s) 145
Bme18I GGWCC 1 cut(s) 659
BmgBI CACGTC 1 cut(s) 923
BmgT120I GGNCC 2 cut(s) 474, 659
BmiI GGNNCC 3 cut(s) 159, 539, 1083
BmsI GCATC 1 cut(s) 342
BpiI GAAGAC 2 cut(s) 208, 947
BpuEI CTTGAG 2 cut(s) 363, 755
BsaWI WCCGGW 2 cut(s) 128, 656
Bsc4I CCNNNNNNNGG 1 cut(s) 170
Bse118I RCCGGY 1 cut(s) 297
Bse3DI GCAATG 1 cut(s) 600
BseGI GGATG 3 cut(s) 333, 938, 1018
BseLI CCNNNNNNNGG 1 cut(s) 170
BseMI GCAATG 1 cut(s) 600
BseMII CTCAG 1 cut(s) 45
Bsh1236I CGCG 3 cut(s) 530, 709, 1037
Bsh1285I CGRYCG 1 cut(s) 918
BshFI GGCC 5 cut(s) 146, 216, 475, 516, 842
BsiEI CGRYCG 1 cut(s) 918
BsiSI CCGG 5 cut(s) 92, 129, 198, 298, 657
BslFI GGGAC 3 cut(s) 370, 388, 973
BslI CCNNNNNNNGG 1 cut(s) 170
BsmAI GTCTC 2 cut(s) 224, 423
BsmBI CGTCTC 1 cut(s) 224
BsmFI GGGAC 3 cut(s) 370, 388, 973
BsnI GGCC 5 cut(s) 146, 216, 475, 516, 842
Bsp143I GATC 7 cut(s) 191, 523, 712, 855, 886, 915, 1081
Bsp68I TCGCGA 1 cut(s) 530
BspACI CCGC 7 cut(s) 143, 195, 324, 337, 699, 707, 1035
BspANI GGCC 5 cut(s) 146, 216, 475, 516, 842
BspCNI CTCAG 1 cut(s) 46
BspFNI CGCG 3 cut(s) 530, 709, 1037
BspLI GGNNCC 3 cut(s) 159, 539, 1083
BspPI GGATC 5 cut(s) 186, 707, 881, 1076, 1089
BsrDI GCAATG 1 cut(s) 600
BsrFI RCCGGY 1 cut(s) 297
BssAI RCCGGY 1 cut(s) 297
BssMI GATC 7 cut(s) 191, 523, 712, 855, 886, 915, 1081
Bst4CI ACNGT 3 cut(s) 678, 722, 992
BstDEI CTNAG 1 cut(s) 54
BstF5I GGATG 3 cut(s) 333, 938, 1018
BstFNI CGCG 3 cut(s) 530, 709, 1037
BstKTI GATC 7 cut(s) 194, 526, 715, 858, 889, 918, 1084
BstMAI GTCTC 2 cut(s) 224, 423
BstMBI GATC 7 cut(s) 191, 523, 712, 855, 886, 915, 1081
BstMCI CGRYCG 1 cut(s) 918
BstMWI GCNNNNNNNGC 4 cut(s) 140, 314, 330, 698
BstNSI RCATGY 1 cut(s) 1110
BstSFI CTRYAG 1 cut(s) 970
BstUI CGCG 3 cut(s) 530, 709, 1037
BstV2I GAAGAC 2 cut(s) 208, 947
BstX2I RGATCY 3 cut(s) 191, 712, 1081
BstYI RGATCY 3 cut(s) 191, 712, 1081
BsuRI GGCC 5 cut(s) 146, 216, 475, 516, 842
BtrI CACGTC 1 cut(s) 923
BtsCI GGATG 3 cut(s) 333, 938, 1018
BtsIMutI CAGTG 1 cut(s) 51
BtuMI TCGCGA 1 cut(s) 530
Cfr10I RCCGGY 1 cut(s) 297
Cfr13I GGNCC 2 cut(s) 474, 659
CpoI CGGWCCG 1 cut(s) 659
Csp6I GTAC 1 cut(s) 411
CspI CGGWCCG 1 cut(s) 659
CviAII CATG 3 cut(s) 302, 547, 1107
CviQI GTAC 1 cut(s) 411
DdeI CTNAG 1 cut(s) 54
DpnI GATC 7 cut(s) 193, 525, 714, 857, 888, 917, 1083
DpnII GATC 7 cut(s) 191, 523, 712, 855, 886, 915, 1081
DrdI GACNNNNNNGTC 1 cut(s) 382
DseDI GACNNNNNNGTC 1 cut(s) 382
EaeI YGGCCR 4 cut(s) 144, 214, 514, 840
EciI GGCGGA 2 cut(s) 184, 326
Eco32I GATATC 2 cut(s) 511, 1077
Eco47I GGWCC 1 cut(s) 659
EcoRV GATATC 2 cut(s) 511, 1077
Esp3I CGTCTC 1 cut(s) 224
FaeI CATG 3 cut(s) 305, 550, 1110
FaqI GGGAC 3 cut(s) 370, 388, 973
FatI CATG 3 cut(s) 301, 546, 1106
FauI CCCGC 1 cut(s) 706
FbaI TGATCA 1 cut(s) 855
FblI GTMKAC 1 cut(s) 834
Fnu4HI GCNGC 1 cut(s) 144
FokI GGATG 3 cut(s) 320, 945, 1025
Fsp4HI GCNGC 1 cut(s) 144
FspBI CTAG 3 cut(s) 44, 875, 1046
GluI GCNGC 1 cut(s) 144
HaeIII GGCC 5 cut(s) 146, 216, 475, 516, 842
HapII CCGG 5 cut(s) 92, 129, 198, 298, 657
Hin1II CATG 3 cut(s) 305, 550, 1110
HindIII AAGCTT 1 cut(s) 690
HinfI GANTC 6 cut(s) 113, 278, 376, 406, 636, 1059
HpaII CCGG 5 cut(s) 92, 129, 198, 298, 657
HphI GGTGA 2 cut(s) 589, 850
Hpy166II GTNNAC 4 cut(s) 51, 674, 835, 1112
Hpy188I TCNGA 6 cut(s) 124, 211, 687, 738, 920, 1081
Hpy188III TCNNGA 3 cut(s) 529, 772, 1117
Hpy8I GTNNAC 4 cut(s) 51, 674, 835, 1112
Hpy99I CGWCG 1 cut(s) 322
HpyAV CCTTC 2 cut(s) 620, 816
HpyCH4III ACNGT 3 cut(s) 678, 722, 992
HpyCH4IV ACGT 1 cut(s) 922
HpyCH4V TGCA 5 cut(s) 26, 179, 333, 456, 470
HpyF10VI GCNNNNNNNGC 4 cut(s) 140, 314, 330, 698
HpyF3I CTNAG 1 cut(s) 54
HpySE526I ACGT 1 cut(s) 922
Hsp92II CATG 3 cut(s) 305, 550, 1110
Ksp22I TGATCA 1 cut(s) 855
Kzo9I GATC 7 cut(s) 191, 523, 712, 855, 886, 915, 1081
LmnI GCTCC 3 cut(s) 131, 287, 629
LpnPI CCDG 7 cut(s) 105, 142, 211, 311, 651, 670, 994
LweI GCATC 1 cut(s) 342
MaeI CTAG 3 cut(s) 44, 875, 1046
MaeII ACGT 1 cut(s) 922
MaeIII GTNAC 1 cut(s) 801
MalI GATC 7 cut(s) 193, 525, 714, 857, 888, 917, 1083
MboI GATC 7 cut(s) 191, 523, 712, 855, 886, 915, 1081
MboII GAAGA 7 cut(s) 201, 213, 631, 674, 801, 952, 969
MfeI CAATTG 1 cut(s) 750
MflI RGATCY 3 cut(s) 191, 712, 1081
MlyI GAGTC 2 cut(s) 370, 400
MmeI TCCRAC 1 cut(s) 265
MnlI CCTC 6 cut(s) 148, 171, 547, 744, 1073, 1098
MseI TTAA 5 cut(s) 102, 263, 402, 581, 608
MslI CAYNNNNRTG 1 cut(s) 210
MspI CCGG 5 cut(s) 92, 129, 198, 298, 657
MunI CAATTG 1 cut(s) 750
MvnI CGCG 3 cut(s) 530, 709, 1037
MwoI GCNNNNNNNGC 4 cut(s) 140, 314, 330, 698
NdeII GATC 7 cut(s) 191, 523, 712, 855, 886, 915, 1081
NlaIII CATG 3 cut(s) 305, 550, 1110
NlaIV GGNNCC 3 cut(s) 159, 539, 1083
NmeAIII GCCGAG 1 cut(s) 818
NruI TCGCGA 1 cut(s) 530
NspI RCATGY 1 cut(s) 1110
PcsI WCGNNNNNNNCGW 1 cut(s) 519
PfeI GAWTC 4 cut(s) 113, 278, 636, 1059
PflMI CCANNNNNTGG 1 cut(s) 170
PkrI GCNGC 1 cut(s) 145
Ple19I CGATCG 1 cut(s) 918
PleI GAGTC 2 cut(s) 370, 400
PpsI GAGTC 2 cut(s) 370, 400
PspN4I GGNNCC 3 cut(s) 159, 539, 1083
PspPI GGNCC 2 cut(s) 474, 659
PsuI RGATCY 3 cut(s) 191, 712, 1081
PvuI CGATCG 1 cut(s) 918
RruI TCGCGA 1 cut(s) 530
RsaI GTAC 1 cut(s) 412
RsaNI GTAC 1 cut(s) 411
RseI CAYNNNNRTG 1 cut(s) 210
Rsr2I CGGWCCG 1 cut(s) 659
RsrII CGGWCCG 1 cut(s) 659
SaqAI TTAA 5 cut(s) 102, 263, 402, 581, 608
SatI GCNGC 1 cut(s) 144
Sau3AI GATC 7 cut(s) 191, 523, 712, 855, 886, 915, 1081
Sau96I GGNCC 2 cut(s) 474, 659
SchI GAGTC 2 cut(s) 370, 400
SfaNI GCATC 1 cut(s) 342
SfcI CTRYAG 1 cut(s) 970
SinI GGWCC 1 cut(s) 659
SmiMI CAYNNNNRTG 1 cut(s) 210
SmlI CTYRAG 2 cut(s) 378, 770
SmoI CTYRAG 2 cut(s) 378, 770
SsiI CCGC 7 cut(s) 143, 195, 324, 337, 699, 707, 1035
SspI AATATT 1 cut(s) 948
SspMI CTAG 3 cut(s) 44, 875, 1046
TaaI ACNGT 3 cut(s) 678, 722, 992
TaiI ACGT 1 cut(s) 925
TaqI TCGA 3 cut(s) 74, 1065, 1073
TaqII GACCGA 1 cut(s) 676
TauI GCSGC 1 cut(s) 146
TfiI GAWTC 4 cut(s) 113, 278, 636, 1059
Tru1I TTAA 5 cut(s) 102, 263, 402, 581, 608
Tru9I TTAA 5 cut(s) 102, 263, 402, 581, 608
TscAI CASTG 1 cut(s) 58
TspDTI ATGAA 4 cut(s) 223, 382, 638, 991
TspGWI ACGGA 1 cut(s) 453
TspRI CASTG 1 cut(s) 58
Van91I CCANNNNNTGG 1 cut(s) 170
VpaK11BI GGWCC 1 cut(s) 659
XapI RAATTY 2 cut(s) 118, 997
XceI RCATGY 1 cut(s) 1110
XmiI GTMKAC 1 cut(s) 834
XspI CTAG 3 cut(s) 44, 875, 1046
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.