Rorug02G0555300

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0010447
Physical Location & Seq
Reverse (-)
68217670 .. 68218748
1079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0555300.1

Sequence Viewer

Length: 498 bp
ATGGCCACCAAGTTCGTCCTCGTCTCGCTTCTCCTCCTCCAGCTTGCCGTAACTGCTTTCTCCGGCGTCGCTCAACAGAACCCTCACCACCAACCACACGGTAAACAGAAGACCGCACATGAGCCAGGGGGTCCAGCAGGGGGAATTCCAAAGCCAGGAAGTAAACCACAGGTTCCGTTCGGAGGAGCAGATGAAGCACCTCACGATGATGGACCTGCATCCGGCGGCCCTGGCAAGAAGTGGTGCATAGCCAAAACTGTCATTCAGAAAAGCCTTTTGAAGAAGGACTTTGATGACTTGTGCAAAGAGGTCGATTGCTCGCCTACAGACGGAAAAGGTTTATGCTATACTGAGTCCATCCAAGCCAAAGCGTCCTTCGCCATGAATCTTAAATATCAAAAGAACGGTAAGAAGGATGCTGACTGCAACTACGGTGGAAGAGCAGAGATTGTCACCAAAGACCCAAGCTGGGCTAAGTGTGTGCTCGTCTCTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

165

Amino Acids

17.46

Weight (kDa)

8.85

Isoelectric Point (pI)

21.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 80 - 148 2.2e-12 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 223
AciI CCGC 2 cut(s) 114, 225
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 144
AcyI GRCGYC 1 cut(s) 66
AfiI CCNNNNNNNGG 6 cut(s) 140, 155, 182, 221, 329, 469
AgsI TTSAA 1 cut(s) 280
AjnI CCWGG 3 cut(s) 124, 154, 229
AjuI GAANNNNNNNTTGG 2 cut(s) 359, 391
AluBI AGCT 2 cut(s) 43, 468
AluI AGCT 2 cut(s) 43, 468
Alw21I GWGCWC 1 cut(s) 486
Alw26I GTCTC 2 cut(s) 28, 493
AoxI GGCC 2 cut(s) 3, 226
ApoI RAATTY 1 cut(s) 144
AspS9I GGNCC 3 cut(s) 131, 212, 227
AsuHPI GGTGA 2 cut(s) 77, 445
AvaII GGWCC 2 cut(s) 131, 212
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 116
Bbv12I GWGCWC 1 cut(s) 486
BccI CCATC 2 cut(s) 203, 365
BceAI ACGGC 1 cut(s) 32
BcgI CGANNNNNNTGC 2 cut(s) 292, 326
BciT130I CCWGG 3 cut(s) 126, 156, 231
BcoDI GTCTC 2 cut(s) 28, 493
BfmI CTRYAG 1 cut(s) 324
BfuAI ACCTGC 1 cut(s) 223
BisI GCNGC 1 cut(s) 226
BlsI GCNGC 1 cut(s) 227
Bme1390I CCNGG 3 cut(s) 126, 156, 231
Bme18I GGWCC 2 cut(s) 131, 212
BmgT120I GGNCC 3 cut(s) 131, 212, 227
BmiI GGNNCC 2 cut(s) 132, 174
BmrFI CCNGG 3 cut(s) 126, 156, 231
BmsI GCATC 2 cut(s) 227, 406
BpiI GAAGAC 1 cut(s) 116
BpmI CTGGAG 1 cut(s) 23
BsaHI GRCGYC 1 cut(s) 66
BsaJI CCNNGG 2 cut(s) 125, 229
Bsc4I CCNNNNNNNGG 6 cut(s) 140, 155, 182, 221, 329, 469
BseBI CCWGG 3 cut(s) 126, 156, 231
BseDI CCNNGG 2 cut(s) 125, 229
BseGI GGATG 3 cut(s) 218, 357, 421
BseLI CCNNNNNNNGG 6 cut(s) 140, 155, 182, 221, 329, 469
BseMII CTCAG 1 cut(s) 342
BseRI GAGGAG 3 cut(s) 23, 26, 198
BseYI CCCAGC 1 cut(s) 468
BshFI GGCC 2 cut(s) 5, 228
BsiHKAI GWGCWC 1 cut(s) 486
BsiSI CCGG 2 cut(s) 63, 222
BslI CCNNNNNNNGG 6 cut(s) 140, 155, 182, 221, 329, 469
BsmAI GTCTC 2 cut(s) 28, 493
BsmBI CGTCTC 2 cut(s) 28, 493
BsnI GGCC 2 cut(s) 5, 228
Bsp1286I GDGCHC 1 cut(s) 486
BspACI CCGC 2 cut(s) 114, 225
BspANI GGCC 2 cut(s) 5, 228
BspCNI CTCAG 1 cut(s) 343
BspLI GGNNCC 2 cut(s) 132, 174
BspMI ACCTGC 1 cut(s) 223
BspQI GCTCTTC 1 cut(s) 433
BssECI CCNNGG 2 cut(s) 125, 229
BssNI GRCGYC 1 cut(s) 66
Bst2UI CCWGG 3 cut(s) 126, 156, 231
Bst4CI ACNGT 4 cut(s) 101, 259, 407, 434
Bst6I CTCTTC 1 cut(s) 433
BstACI GRCGYC 1 cut(s) 66
BstC8I GCNNGC 2 cut(s) 45, 320
BstDEI CTNAG 2 cut(s) 351, 474
BstF5I GGATG 3 cut(s) 218, 357, 421
BstMAI GTCTC 2 cut(s) 28, 493
BstMWI GCNNNNNNNGC 4 cut(s) 53, 194, 231, 377
BstNI CCWGG 3 cut(s) 126, 156, 231
BstSCI CCNGG 3 cut(s) 124, 154, 229
BstSFI CTRYAG 1 cut(s) 324
BstV2I GAAGAC 1 cut(s) 116
BsuRI GGCC 2 cut(s) 5, 228
BtsCI GGATG 3 cut(s) 218, 357, 421
BveI ACCTGC 1 cut(s) 223
Cac8I GCNNGC 2 cut(s) 45, 320
Cfr13I GGNCC 3 cut(s) 131, 212, 227
CseI GACGC 2 cut(s) 55, 360
CspCI CAANNNNNGTGG 2 cut(s) 415, 450
CviAII CATG 2 cut(s) 119, 382
DdeI CTNAG 2 cut(s) 351, 474
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 433
EarI CTCTTC 1 cut(s) 433
Eco47I GGWCC 2 cut(s) 131, 212
EcoRI GAATTC 1 cut(s) 144
EcoRII CCWGG 3 cut(s) 124, 154, 229
Esp3I CGTCTC 2 cut(s) 28, 493
FaeI CATG 2 cut(s) 122, 385
FaiI YATR 5 cut(s) 120, 248, 343, 348, 383
FalI AAGNNNNNCTT 2 cut(s) 272, 304
FatI CATG 2 cut(s) 118, 381
Fnu4HI GCNGC 1 cut(s) 226
FokI GGATG 3 cut(s) 205, 344, 428
Fsp4HI GCNGC 1 cut(s) 226
GluI GCNGC 1 cut(s) 226
GsaI CCCAGC 1 cut(s) 472
GsuI CTGGAG 1 cut(s) 23
HaeIII GGCC 2 cut(s) 5, 228
HapII CCGG 2 cut(s) 63, 222
HgaI GACGC 2 cut(s) 55, 360
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 2 cut(s) 122, 385
HinfI GANTC 2 cut(s) 353, 385
HpaII CCGG 2 cut(s) 63, 222
HphI GGTGA 2 cut(s) 77, 445
Hpy166II GTNNAC 2 cut(s) 104, 164
Hpy188I TCNGA 2 cut(s) 182, 267
Hpy188III TCNNGA 1 cut(s) 203
Hpy8I GTNNAC 2 cut(s) 104, 164
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 3 cut(s) 277, 385, 406
HpyCH4III ACNGT 4 cut(s) 101, 259, 407, 434
HpyCH4V TGCA 4 cut(s) 218, 246, 303, 426
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 194, 231, 377
HpyF3I CTNAG 2 cut(s) 351, 474
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 2 cut(s) 122, 385
LguI GCTCTTC 1 cut(s) 433
LmnI GCTCC 1 cut(s) 185
LweI GCATC 2 cut(s) 227, 406
MaeIII GTNAC 2 cut(s) 49, 451
MboII GAAGA 3 cut(s) 121, 292, 450
MhlI GDGCHC 1 cut(s) 486
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 144, 493
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 362
MnlI CCTC 7 cut(s) 29, 44, 47, 93, 176, 210, 301
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 390, 496
MslI CAYNNNNRTG 1 cut(s) 207
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 63, 222
MspR9I CCNGG 3 cut(s) 126, 156, 231
MvaI CCWGG 3 cut(s) 126, 156, 231
MwoI GCNNNNNNNGC 4 cut(s) 53, 194, 231, 377
NlaIII CATG 2 cut(s) 122, 385
NlaIV GGNNCC 2 cut(s) 132, 174
NmuCI GTSAC 1 cut(s) 451
PciSI GCTCTTC 1 cut(s) 433
PfeI GAWTC 1 cut(s) 385
PkrI GCNGC 1 cut(s) 227
PleI GAGTC 1 cut(s) 361
PpsI GAGTC 1 cut(s) 361
Psp6I CCWGG 3 cut(s) 124, 154, 229
PspFI CCCAGC 1 cut(s) 468
PspGI CCWGG 3 cut(s) 124, 154, 229
PspN4I GGNNCC 2 cut(s) 132, 174
PspPI GGNCC 3 cut(s) 131, 212, 227
RseI CAYNNNNRTG 1 cut(s) 207
SapI GCTCTTC 1 cut(s) 433
SaqAI TTAA 2 cut(s) 390, 496
SatI GCNGC 1 cut(s) 226
Sau96I GGNCC 3 cut(s) 131, 212, 227
SchI GAGTC 1 cut(s) 362
ScrFI CCNGG 3 cut(s) 126, 156, 231
SduI GDGCHC 1 cut(s) 486
SetI ASST 7 cut(s) 45, 174, 202, 217, 312, 340, 470
SfaNI GCATC 2 cut(s) 227, 406
SfcI CTRYAG 1 cut(s) 324
SinI GGWCC 2 cut(s) 131, 212
SmiMI CAYNNNNRTG 1 cut(s) 207
Sse9I AATT 2 cut(s) 144, 493
SsiI CCGC 2 cut(s) 114, 225
StyD4I CCNGG 3 cut(s) 124, 154, 229
TaaI ACNGT 4 cut(s) 101, 259, 407, 434
TaqI TCGA 1 cut(s) 312
TasI AATT 2 cut(s) 144, 493
TauI GCSGC 1 cut(s) 228
TfiI GAWTC 1 cut(s) 385
Tru1I TTAA 2 cut(s) 390, 496
Tru9I TTAA 2 cut(s) 390, 496
TseFI GTSAC 1 cut(s) 451
Tsp45I GTSAC 1 cut(s) 451
TspDTI ATGAA 2 cut(s) 207, 398
TspGWI ACGGA 2 cut(s) 165, 345
VpaK11BI GGWCC 2 cut(s) 131, 212
XapI RAATTY 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.