RLG00000022028

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010447
Physical Location & Seq
Reverse (-)
80122708 .. 80124234
1527 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022028

Sequence Viewer

Length: 1089 bp
ATGGATCTTGTGAGGCTATTTTTTGCAGTACTTGGCATTTTAGCTTTGGGAGTGAATGCTGGTGGTTCTGGGAGTTGTAGGTTTCCGGCCATATACAACTTTGGTGACTCAAACTCCGACACCGGCGCTATTTCAGCCGCCATAGAAGTGGTTCCTCCTCCCAATGGTGAAAGTTTCTTTGGACATTCTTCTGGACGCTTTTCTGATGGTCGCCTTATTATAGATTTCATAGCTGAGAAGTTGCAGCTACCATATCTCAGTCCATACTTGGACTCACTTGGGACTAATTTCAGGCATGGTGCAAATTTTGCAACCGGCGGTTCATCCATTAGGCCAGGCGGTTATAGCCCGTTTCATCTCGGCATTCAAGTTTCACAGTTCGTAAGATTCAAGTCTCAATCCATAGCCCTCTACAAGCAACAAACTCAAAGACTTCCTAGGCCGGAGGACTTCTCAAAAGCTTTGTACACAATTGATATTGGTCAGAATGATCTTGCCTATGGTTTTCAACACGGGACGATTCAGCAAGTTCGAGCCTCCATTCCTGATATTCTCACTCAGTTAGACCAAGCCATGAATAAACTATACAAAGAAGGGGCAAGGTTTTTTTGGGTGCATAACACAGGCCCTCATGGGTGCTTGCCCTATAATGTTATCTATGACAAATCAAAGCCAGGTAATCTGGACCGGATTGGCTGCGTGAAGCCTACCAACGAGGTAGCTCAGGAGTTCAATCGGCAGCTTAAGAACAGCGTATTACACTTGAGGAAGCAGCTTCCTCTTGCAGTATTCACTTATGTCGATGTGTATTCAGCTAAATATACAGTGATCAGCAACGCGAAAAATGAAGGTTTTGTGGATCCATTCGACTTCTGCTGTGGCAGTTACAAGGGGTACCATATTGATTGTGGAAAGAAGGCCATAGTGAATGGAACGGTATATGGCAACCCTTGTAATAATCCATCAAGACATATTAGTTGGGATGGCATACACTACTCTCAGGCAGCAAACATATTGCTTGCCAATCAAATTCTTAACGGCTCACTATCTGATCCACCAGTTTCAATCACAGAGGCTTGTCATGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

363

Amino Acids

39.72

Weight (kDa)

8.33

Isoelectric Point (pI)

32.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 31 - 344 1.7e-46 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 894
AccB1I GGYRCC 1 cut(s) 894
AccII CGCG 1 cut(s) 839
AciI CCGC 3 cut(s) 138, 318, 339
AclWI GGATC 4 cut(s) 12, 854, 867, 1046
AcoI YGGCCR 1 cut(s) 87
AcsI RAATTY 2 cut(s) 304, 1029
AfaI GTAC 3 cut(s) 30, 467, 896
AfiI CCNNNNNNNGG 1 cut(s) 164
AflII CTTAAG 1 cut(s) 743
AgsI TTSAA 5 cut(s) 368, 391, 509, 733, 1065
AjnI CCWGG 2 cut(s) 334, 673
AjuI GAANNNNNNNTTGG 2 cut(s) 162, 194
AluBI AGCT 8 cut(s) 44, 233, 247, 461, 722, 742, 775, 815
AluI AGCT 8 cut(s) 44, 233, 247, 461, 722, 742, 775, 815
Alw26I GTCTC 1 cut(s) 399
AlwI GGATC 4 cut(s) 12, 854, 867, 1046
AoxI GGCC 5 cut(s) 87, 332, 440, 625, 918
ApeKI GCWGC 5 cut(s) 244, 696, 739, 772, 1004
ApoI RAATTY 2 cut(s) 304, 1029
Asp700I GAANNNNTTC 1 cut(s) 150
Asp718I GGTACC 1 cut(s) 894
AspA2I CCTAGG 1 cut(s) 437
AspLEI GCGC 1 cut(s) 128
AspS9I GGNCC 2 cut(s) 626, 685
AsuHPI GGTGA 2 cut(s) 116, 179
AvaII GGWCC 1 cut(s) 685
AvrII CCTAGG 1 cut(s) 437
BamHI GGATCC 1 cut(s) 859
BanI GGYRCC 1 cut(s) 894
BbvI GCAGC 5 cut(s) 256, 683, 751, 784, 1016
BccI CCATC 3 cut(s) 200, 970, 977
BceAI ACGGC 1 cut(s) 1054
BciT130I CCWGG 2 cut(s) 336, 675
BclI TGATCA 1 cut(s) 828
BcoDI GTCTC 1 cut(s) 399
BfaI CTAG 1 cut(s) 438
BfoI RGCGCY 1 cut(s) 129
BfrI CTTAAG 1 cut(s) 743
BisI GCNGC 6 cut(s) 138, 245, 697, 740, 773, 1005
BlnI CCTAGG 1 cut(s) 437
BlsI GCNGC 6 cut(s) 139, 246, 698, 741, 774, 1006
BmcAI AGTACT 1 cut(s) 30
Bme1390I CCNGG 2 cut(s) 336, 675
Bme18I GGWCC 1 cut(s) 685
BmgT120I GGNCC 2 cut(s) 626, 685
BmiI GGNNCC 3 cut(s) 153, 861, 896
BmrFI CCNGG 2 cut(s) 336, 675
BplI GAGNNNNNCTC 2 cut(s) 437, 469
Bpu10I CCTNAGC 1 cut(s) 723
BpuEI CTTGAG 1 cut(s) 784
BsaJI CCNNGG 1 cut(s) 437
BsaWI WCCGGW 1 cut(s) 687
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 1 cut(s) 164
Bse118I RCCGGY 2 cut(s) 122, 314
Bse1I ACTGG 1 cut(s) 1058
BseBI CCWGG 2 cut(s) 336, 675
BseDI CCNNGG 1 cut(s) 437
BseGI GGATG 2 cut(s) 323, 988
BseLI CCNNNNNNNGG 1 cut(s) 164
BseMII CTCAG 5 cut(s) 225, 271, 572, 737, 1013
BseNI ACTGG 1 cut(s) 1058
BseRI GAGGAG 1 cut(s) 147
BseXI GCAGC 5 cut(s) 256, 683, 751, 784, 1016
Bsh1236I CGCG 1 cut(s) 839
BshFI GGCC 5 cut(s) 89, 334, 442, 627, 920
BshNI GGYRCC 1 cut(s) 894
BsiSI CCGG 5 cut(s) 86, 123, 315, 443, 688
BslFI GGGAC 2 cut(s) 295, 529
BslI CCNNNNNNNGG 1 cut(s) 164
BsmAI GTCTC 1 cut(s) 399
BsmFI GGGAC 2 cut(s) 295, 529
BsmI GAATGC 2 cut(s) 61, 363
BsnI GGCC 5 cut(s) 89, 334, 442, 627, 920
Bsp1407I TGTACA 1 cut(s) 465
Bsp143I GATC 5 cut(s) 4, 490, 828, 859, 1051
BspACI CCGC 3 cut(s) 138, 318, 339
BspANI GGCC 5 cut(s) 89, 334, 442, 627, 920
BspCNI CTCAG 5 cut(s) 226, 270, 571, 736, 1012
BspFNI CGCG 1 cut(s) 839
BspLI GGNNCC 3 cut(s) 153, 861, 896
BspPI GGATC 4 cut(s) 12, 854, 867, 1046
BspT107I GGYRCC 1 cut(s) 894
BspTI CTTAAG 1 cut(s) 743
BsrFI RCCGGY 2 cut(s) 122, 314
BsrGI TGTACA 1 cut(s) 465
BsrI ACTGG 1 cut(s) 1058
BssAI RCCGGY 2 cut(s) 122, 314
BssECI CCNNGG 1 cut(s) 437
BssMI GATC 5 cut(s) 4, 490, 828, 859, 1051
BssT1I CCWWGG 1 cut(s) 437
Bst2UI CCWGG 2 cut(s) 336, 675
Bst4CI ACNGT 3 cut(s) 378, 826, 937
BstAFI CTTAAG 1 cut(s) 743
BstAPI GCANNNNNTGC 1 cut(s) 308
BstAUI TGTACA 1 cut(s) 465
BstC8I GCNNGC 2 cut(s) 641, 1020
BstDEI CTNAG 5 cut(s) 234, 257, 558, 723, 999
BstF5I GGATG 2 cut(s) 323, 988
BstFNI CGCG 1 cut(s) 839
BstH2I RGCGCY 1 cut(s) 129
BstHHI GCGC 1 cut(s) 128
BstKTI GATC 5 cut(s) 7, 493, 831, 862, 1054
BstMAI GTCTC 1 cut(s) 399
BstMBI GATC 5 cut(s) 4, 490, 828, 859, 1051
BstMWI GCNNNNNNNGC 3 cut(s) 134, 308, 345
BstNI CCWGG 2 cut(s) 336, 675
BstSCI CCNGG 2 cut(s) 334, 673
BstUI CGCG 1 cut(s) 839
BstV1I GCAGC 5 cut(s) 256, 683, 751, 784, 1016
BstX2I RGATCY 2 cut(s) 4, 859
BstXI CCANNNNNNTGG 1 cut(s) 148
BstYI RGATCY 2 cut(s) 4, 859
BsuRI GGCC 5 cut(s) 89, 334, 442, 627, 920
BtsCI GGATG 2 cut(s) 323, 988
BtsIMutI CAGTG 1 cut(s) 831
Cac8I GCNNGC 2 cut(s) 641, 1020
CfoI GCGC 1 cut(s) 128
Cfr10I RCCGGY 2 cut(s) 122, 314
Cfr13I GGNCC 2 cut(s) 626, 685
CseI GACGC 1 cut(s) 204
Csp6I GTAC 3 cut(s) 29, 466, 895
CviAII CATG 4 cut(s) 296, 574, 632, 1082
CviQI GTAC 3 cut(s) 29, 466, 895
DdeI CTNAG 5 cut(s) 234, 257, 558, 723, 999
DpnI GATC 5 cut(s) 6, 492, 830, 861, 1053
DpnII GATC 5 cut(s) 4, 490, 828, 859, 1051
EaeI YGGCCR 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 437
Eco47I GGWCC 1 cut(s) 685
EcoO109I RGGNCCY 1 cut(s) 626
EcoRII CCWGG 2 cut(s) 334, 673
EcoT14I CCWWGG 1 cut(s) 437
ErhI CCWWGG 1 cut(s) 437
FaeI CATG 4 cut(s) 299, 577, 635, 1085
FaqI GGGAC 2 cut(s) 295, 529
FatI CATG 4 cut(s) 295, 573, 631, 1081
FbaI TGATCA 1 cut(s) 828
Fnu4HI GCNGC 6 cut(s) 138, 245, 697, 740, 773, 1005
FokI GGATG 2 cut(s) 310, 995
Fsp4HI GCNGC 6 cut(s) 138, 245, 697, 740, 773, 1005
FspBI CTAG 1 cut(s) 438
GlaI GCGC 1 cut(s) 127
GluI GCNGC 6 cut(s) 138, 245, 697, 740, 773, 1005
HaeII RGCGCY 1 cut(s) 129
HaeIII GGCC 5 cut(s) 89, 334, 442, 627, 920
HapII CCGG 5 cut(s) 86, 123, 315, 443, 688
HgaI GACGC 1 cut(s) 204
HhaI GCGC 1 cut(s) 128
Hin1II CATG 4 cut(s) 299, 577, 635, 1085
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HindIII AAGCTT 1 cut(s) 459
HinfI GANTC 4 cut(s) 107, 272, 387, 520
HpaII CCGG 5 cut(s) 86, 123, 315, 443, 688
HphI GGTGA 2 cut(s) 116, 179
Hpy166II GTNNAC 1 cut(s) 468
Hpy188I TCNGA 4 cut(s) 118, 205, 486, 1051
Hpy188III TCNNGA 5 cut(s) 192, 545, 683, 725, 966
Hpy8I GTNNAC 1 cut(s) 468
HpyAV CCTTC 3 cut(s) 587, 842, 910
HpyCH4III ACNGT 3 cut(s) 378, 826, 937
HpyCH4V TGCA 6 cut(s) 26, 244, 302, 311, 616, 785
HpyF10VI GCNNNNNNNGC 3 cut(s) 134, 308, 345
HpyF3I CTNAG 5 cut(s) 234, 257, 558, 723, 999
Hsp92II CATG 4 cut(s) 299, 577, 635, 1085
HspAI GCGC 1 cut(s) 126
KpnI GGTACC 1 cut(s) 898
Ksp22I TGATCA 1 cut(s) 828
Kzo9I GATC 5 cut(s) 4, 490, 828, 859, 1051
Lsp1109I GCAGC 5 cut(s) 256, 683, 751, 784, 1016
MaeI CTAG 1 cut(s) 438
MaeIII GTNAC 2 cut(s) 104, 884
MalI GATC 5 cut(s) 6, 492, 830, 861, 1053
MboI GATC 5 cut(s) 4, 490, 828, 859, 1051
MboII GAAGA 1 cut(s) 180
MfeI CAATTG 1 cut(s) 471
MflI RGATCY 2 cut(s) 4, 859
MluCI AATT 4 cut(s) 286, 304, 471, 1029
MlyI GAGTC 2 cut(s) 101, 266
MmeI TCCRAC 1 cut(s) 141
MroXI GAANNNNTTC 1 cut(s) 150
MseI TTAA 2 cut(s) 744, 1035
MslI CAYNNNNRTG 1 cut(s) 146
MspCI CTTAAG 1 cut(s) 743
MspI CCGG 5 cut(s) 86, 123, 315, 443, 688
MspR9I CCNGG 2 cut(s) 336, 675
MunI CAATTG 1 cut(s) 471
Mva1269I GAATGC 2 cut(s) 61, 363
MvaI CCWGG 2 cut(s) 336, 675
MvnI CGCG 1 cut(s) 839
MwoI GCNNNNNNNGC 3 cut(s) 134, 308, 345
NdeII GATC 5 cut(s) 4, 490, 828, 859, 1051
NlaIII CATG 4 cut(s) 299, 577, 635, 1085
NlaIV GGNNCC 3 cut(s) 153, 861, 896
NmeAIII GCCGAG 1 cut(s) 339
NmuCI GTSAC 1 cut(s) 104
PctI GAATGC 2 cut(s) 61, 363
PdmI GAANNNNTTC 1 cut(s) 150
PfeI GAWTC 2 cut(s) 387, 520
PkrI GCNGC 6 cut(s) 139, 246, 698, 741, 774, 1006
PleI GAGTC 2 cut(s) 101, 266
PpsI GAGTC 2 cut(s) 101, 266
Psp6I CCWGG 2 cut(s) 334, 673
PspGI CCWGG 2 cut(s) 334, 673
PspN4I GGNNCC 3 cut(s) 153, 861, 896
PspPI GGNCC 2 cut(s) 626, 685
PsuI RGATCY 2 cut(s) 4, 859
RsaI GTAC 3 cut(s) 30, 467, 896
RsaNI GTAC 3 cut(s) 29, 466, 895
RseI CAYNNNNRTG 1 cut(s) 146
SaqAI TTAA 2 cut(s) 744, 1035
SatI GCNGC 6 cut(s) 138, 245, 697, 740, 773, 1005
Sau3AI GATC 5 cut(s) 4, 490, 828, 859, 1051
Sau96I GGNCC 2 cut(s) 626, 685
ScaI AGTACT 1 cut(s) 30
SchI GAGTC 2 cut(s) 101, 266
ScrFI CCNGG 2 cut(s) 336, 675
SgrAI CRCCGGYG 1 cut(s) 122
SinI GGWCC 1 cut(s) 685
SmiMI CAYNNNNRTG 1 cut(s) 146
SmlI CTYRAG 2 cut(s) 743, 763
SmoI CTYRAG 2 cut(s) 743, 763
Sse9I AATT 4 cut(s) 286, 304, 471, 1029
SsiI CCGC 3 cut(s) 138, 318, 339
SspMI CTAG 1 cut(s) 438
StyD4I CCNGG 2 cut(s) 334, 673
StyI CCWWGG 1 cut(s) 437
TaaI ACNGT 3 cut(s) 378, 826, 937
TaqI TCGA 3 cut(s) 532, 801, 867
TasI AATT 4 cut(s) 286, 304, 471, 1029
TatI WGTACW 2 cut(s) 28, 465
TauI GCSGC 1 cut(s) 140
TfiI GAWTC 2 cut(s) 387, 520
Tru1I TTAA 2 cut(s) 744, 1035
Tru9I TTAA 2 cut(s) 744, 1035
TscAI CASTG 1 cut(s) 831
TseFI GTSAC 1 cut(s) 104
TseI GCWGC 5 cut(s) 244, 696, 739, 772, 1004
Tsp45I GTSAC 1 cut(s) 104
TspDTI ATGAA 5 cut(s) 217, 312, 344, 590, 861
TspRI CASTG 1 cut(s) 831
Vha464I CTTAAG 1 cut(s) 743
VpaK11BI GGWCC 1 cut(s) 685
XapI RAATTY 2 cut(s) 304, 1029
XcmI CCANNNNNNNNNTGG 1 cut(s) 905
XmaJI CCTAGG 1 cut(s) 437
XmnI GAANNNNTTC 1 cut(s) 150
XspI CTAG 1 cut(s) 438
ZrmI AGTACT 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.