MD09G1033000.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0010447
Physical Location & Seq
Forward (+)
2005142 .. 2007161
2020 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1033000.v1.1.491

Sequence Viewer

Length: 1137 bp
ATGGATTTTCCCAGGCTACTTTGTGCAGCTGTGCTTCTTGTTTTGGGGTTGCTGCTGGGCGGACAGAAGGCGGTGAATGGTGGTGCTGATGGCGGTTCCGGGAGTTGTCGCTTTCCAGCCATTTACAACTTTGGGGACTCAAACTCTGACACTGGGGCTATCTCAGCTGCCATATCCGAAGTTCCTCCTCCTAATGGTGAAACCTTCTTTGGAAAACCTTCCGGGCGCTTGTCTGATGGCCGTCTTATAATCGACTTTATCGCTGAGAAGTTGCAGATACCATATCTTAGTCCATACCTGGACTCACTTGGGACTAATTTCCTGCATGGTGCGAATTTCGCAACCGGTGGTTCATCGGTTAGGCCGGGCGGTTATAGTCCGTTTCATCTTGGCATTCAAATTTCCCAGTTCATAAGATTCAAGTCTCACAGCATTGCTCTCTATAAACAACTTCATTCAACCAGAAGACTACCTAGGCCGGAGGACTTCCCGAAGGCTCTCTACACTTTCGATATTGGTCAGAATGATCTGGCCTACGGCATCCAACACGGGACGATAGAAGAAGTTAGAGCCTCCATTCCTGCCATCCTGAGCCAGTTATCGCAAGCAATATCCCAACTATACAAGGAAGGAGCTAGGTTTTTCTGGGTGCATAACACAGGCCCTCAAGGATGCTTGCCCTATAGCGTTATTTACGACAAATCGAAGCCTGGTAATCTGGACAAGAGCGGCTGCGTGAAGCCTCTCAATGCGGTGGCTCAGGAGTTCAACCGGCAGCTTAAGAACAGCATAATGCTGTTGAGATCACTGCTCCCTCTTGCAGCATTCACGTACGTCGATGTGTACTCAGCTAAATACACTCTCATTTCTGATGCTAAAAAACAAGGTTTTGTTGATCCTTTCGACTTTTGCTGCGGCAGTTACTACGGATTCCACATTGATTGCGGAAGGAAAGCCGTGGTGAATGGAACTGTGTATGGCAATCCTTGCAGCAGTCCGACAACGCATATCAGTTGGGATGGGATACACTACTCCCAGGCCGCCAATGCACTGATTGCCAGTCGCATTCTCAACGGCTCACTCTCTGACCCCCCGGTTTCAATCACGCACGCTTGTCATAGTTCCAAGAATACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

379

Amino Acids

40.86

Weight (kDa)

8.62

Isoelectric Point (pI)

35.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 41 - 356 6.9e-43 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 248
AccBSI CCGCTC 1 cut(s) 729
AciI CCGC 9 cut(s) 60, 71, 93, 369, 729, 752, 915, 945, 1041
AclWI GGATC 1 cut(s) 890
AcoI YGGCCR 1 cut(s) 238
AcsI RAATTY 2 cut(s) 334, 399
AfaI GTAC 2 cut(s) 833, 845
AfiI CCNNNNNNNGG 1 cut(s) 194
AflII CTTAAG 1 cut(s) 779
AgeI ACCGGT 1 cut(s) 344
AgsI TTSAA 5 cut(s) 398, 421, 459, 769, 1101
AjnI CCWGG 4 cut(s) 11, 297, 709, 1035
AjuI GAANNNNNNNTTGG 2 cut(s) 192, 224
AluBI AGCT 5 cut(s) 29, 167, 635, 778, 851
AluI AGCT 5 cut(s) 29, 167, 635, 778, 851
Alw26I GTCTC 1 cut(s) 429
AlwI GGATC 1 cut(s) 890
AoxI GGCC 6 cut(s) 238, 362, 476, 531, 661, 1038
ApeKI GCWGC 8 cut(s) 26, 52, 167, 732, 775, 821, 912, 990
ApoI RAATTY 2 cut(s) 334, 399
AsiGI ACCGGT 1 cut(s) 344
Asp700I GAANNNNTTC 1 cut(s) 217
AspA2I CCTAGG 1 cut(s) 473
AspLEI GCGC 1 cut(s) 228
AspS9I GGNCC 1 cut(s) 662
AsuC2I CCSGG 4 cut(s) 100, 223, 366, 1094
AsuHPI GGTGA 3 cut(s) 85, 209, 973
AvrII CCTAGG 1 cut(s) 473
BarI GAAGNNNNNNTAC 2 cut(s) 485, 517
BbsI GAAGAC 1 cut(s) 472
BbvI GCAGC 8 cut(s) 38, 39, 154, 719, 787, 833, 899, 1002
BccI CCATC 4 cut(s) 83, 230, 593, 1013
BceAI ACGGC 4 cut(s) 225, 553, 941, 1090
BciT130I CCWGG 4 cut(s) 13, 299, 711, 1037
BciVI GTATCC 1 cut(s) 1017
BcnI CCSGG 4 cut(s) 100, 223, 366, 1094
BcoDI GTCTC 1 cut(s) 429
BfaI CTAG 2 cut(s) 474, 636
BfmI CTRYAG 1 cut(s) 682
BfoI RGCGCY 1 cut(s) 229
BfrI CTTAAG 1 cut(s) 779
BfuI GTATCC 1 cut(s) 1017
BlnI CCTAGG 1 cut(s) 473
Bme1390I CCNGG 8 cut(s) 13, 100, 223, 299, 366, 711, 1037, 1094
BmgT120I GGNCC 1 cut(s) 662
BmiI GGNNCC 1 cut(s) 97
BmrFI CCNGG 8 cut(s) 13, 100, 223, 299, 366, 711, 1037, 1094
BmrI ACTGGG 2 cut(s) 162, 400
BmsI GCATC 3 cut(s) 549, 662, 862
BmuI ACTGGG 2 cut(s) 162, 400
BpiI GAAGAC 1 cut(s) 472
Bpu10I CCTNAGC 2 cut(s) 590, 759
BpuEI CTTGAG 1 cut(s) 651
BpuMI CCSGG 4 cut(s) 100, 223, 366, 1094
BsaAI YACGTR 1 cut(s) 831
BsaJI CCNNGG 5 cut(s) 11, 473, 957, 1035, 1092
BsaWI WCCGGW 1 cut(s) 344
Bsc4I CCNNNNNNNGG 1 cut(s) 194
Bse118I RCCGGY 2 cut(s) 344, 771
Bse1I ACTGG 4 cut(s) 157, 406, 595, 1059
Bse3DI GCAATG 1 cut(s) 432
BseBI CCWGG 4 cut(s) 13, 299, 711, 1037
BseDI CCNNGG 5 cut(s) 11, 473, 957, 1035, 1092
BseGI GGATG 4 cut(s) 540, 585, 677, 1024
BseLI CCNNNNNNNGG 1 cut(s) 194
BseMI GCAATG 1 cut(s) 432
BseMII CTCAG 5 cut(s) 177, 255, 581, 773, 861
BseNI ACTGG 4 cut(s) 157, 406, 595, 1059
BseRI GAGGAG 1 cut(s) 177
BseXI GCAGC 8 cut(s) 38, 39, 154, 719, 787, 833, 899, 1002
BseYI CCCAGC 1 cut(s) 55
BsgI GTGCAG 1 cut(s) 45
BshFI GGCC 6 cut(s) 240, 364, 478, 533, 663, 1040
BshTI ACCGGT 1 cut(s) 344
BsiSI CCGG 7 cut(s) 99, 222, 345, 365, 479, 772, 1094
BsiWI CGTACG 1 cut(s) 831
BslFI GGGAC 3 cut(s) 149, 325, 565
BslI CCNNNNNNNGG 1 cut(s) 194
BsmAI GTCTC 1 cut(s) 429
BsmFI GGGAC 3 cut(s) 149, 325, 565
BsmI GAATGC 3 cut(s) 393, 824, 1065
BsnI GGCC 6 cut(s) 240, 364, 478, 533, 663, 1040
Bsp143I GATC 3 cut(s) 526, 803, 895
BspACI CCGC 9 cut(s) 60, 71, 93, 369, 729, 752, 915, 945, 1041
BspANI GGCC 6 cut(s) 240, 364, 478, 533, 663, 1040
BspCNI CTCAG 5 cut(s) 176, 256, 582, 772, 860
BspLI GGNNCC 1 cut(s) 97
BspPI GGATC 1 cut(s) 890
BspTI CTTAAG 1 cut(s) 779
BsrBI CCGCTC 1 cut(s) 729
BsrDI GCAATG 1 cut(s) 432
BsrFI RCCGGY 2 cut(s) 344, 771
BsrI ACTGG 4 cut(s) 157, 406, 595, 1059
BssAI RCCGGY 2 cut(s) 344, 771
BssECI CCNNGG 5 cut(s) 11, 473, 957, 1035, 1092
BssMI GATC 3 cut(s) 526, 803, 895
BssT1I CCWWGG 1 cut(s) 473
Bst2UI CCWGG 4 cut(s) 13, 299, 711, 1037
Bst4CI ACNGT 1 cut(s) 973
BstAFI CTTAAG 1 cut(s) 779
BstAPI GCANNNNNTGC 2 cut(s) 987, 1055
BstBAI YACGTR 1 cut(s) 831
BstC8I GCNNGC 3 cut(s) 606, 677, 1110
BstDEI CTNAG 6 cut(s) 163, 264, 287, 590, 759, 847
BstDSI CCRYGG 1 cut(s) 957
BstF5I GGATG 4 cut(s) 540, 585, 677, 1024
BstH2I RGCGCY 1 cut(s) 229
BstHHI GCGC 1 cut(s) 228
BstKTI GATC 3 cut(s) 529, 806, 898
BstMAI GTCTC 1 cut(s) 429
BstMBI GATC 3 cut(s) 526, 803, 895
BstMWI GCNNNNNNNGC 5 cut(s) 164, 338, 987, 1046, 1055
BstNI CCWGG 4 cut(s) 13, 299, 711, 1037
BstSCI CCNGG 8 cut(s) 11, 98, 221, 297, 364, 709, 1035, 1092
BstSFI CTRYAG 1 cut(s) 682
BstV1I GCAGC 8 cut(s) 38, 39, 154, 719, 787, 833, 899, 1002
BstV2I GAAGAC 1 cut(s) 472
BsuI GTATCC 1 cut(s) 1017
BsuRI GGCC 6 cut(s) 240, 364, 478, 533, 663, 1040
BtgI CCRYGG 1 cut(s) 957
BtsCI GGATG 4 cut(s) 540, 585, 677, 1024
BtsI GCAGTG 1 cut(s) 806
BtsIMutI CAGTG 3 cut(s) 150, 806, 1049
Cac8I GCNNGC 3 cut(s) 606, 677, 1110
CfoI GCGC 1 cut(s) 228
Cfr10I RCCGGY 2 cut(s) 344, 771
Cfr13I GGNCC 1 cut(s) 662
Csp6I GTAC 2 cut(s) 832, 844
CspAI ACCGGT 1 cut(s) 344
CspCI CAANNNNNGTGG 2 cut(s) 923, 958
CviAII CATG 2 cut(s) 326, 1134
CviQI GTAC 2 cut(s) 832, 844
DdeI CTNAG 6 cut(s) 163, 264, 287, 590, 759, 847
DpnI GATC 3 cut(s) 528, 805, 897
DpnII GATC 3 cut(s) 526, 803, 895
EaeI YGGCCR 1 cut(s) 238
EciI GGCGGA 1 cut(s) 75
Eco130I CCWWGG 1 cut(s) 473
EcoO109I RGGNCCY 1 cut(s) 662
EcoRII CCWGG 4 cut(s) 11, 297, 709, 1035
EcoT14I CCWWGG 1 cut(s) 473
ErhI CCWWGG 1 cut(s) 473
FaeI CATG 2 cut(s) 329, 1137
FaqI GGGAC 3 cut(s) 149, 325, 565
FatI CATG 2 cut(s) 325, 1133
FokI GGATG 4 cut(s) 527, 572, 684, 1031
FspBI CTAG 2 cut(s) 474, 636
GlaI GCGC 1 cut(s) 227
GsaI CCCAGC 1 cut(s) 59
HaeII RGCGCY 1 cut(s) 229
HaeIII GGCC 6 cut(s) 240, 364, 478, 533, 663, 1040
HapII CCGG 7 cut(s) 99, 222, 345, 365, 479, 772, 1094
HhaI GCGC 1 cut(s) 228
Hin1II CATG 2 cut(s) 329, 1137
Hin6I GCGC 1 cut(s) 226
HinP1I GCGC 1 cut(s) 226
HinfI GANTC 4 cut(s) 137, 302, 417, 930
HpaII CCGG 7 cut(s) 99, 222, 345, 365, 479, 772, 1094
HphI GGTGA 3 cut(s) 85, 209, 973
Hpy166II GTNNAC 1 cut(s) 844
Hpy188I TCNGA 7 cut(s) 148, 178, 235, 522, 871, 999, 1087
Hpy188III TCNNGA 4 cut(s) 490, 589, 719, 761
Hpy8I GTNNAC 1 cut(s) 844
Hpy99I CGWCG 1 cut(s) 839
HpyAV CCTTC 6 cut(s) 61, 214, 228, 487, 623, 942
HpyCH4III ACNGT 1 cut(s) 973
HpyCH4IV ACGT 2 cut(s) 830, 834
HpyCH4V TGCA 7 cut(s) 26, 274, 325, 652, 821, 990, 1049
HpyF10VI GCNNNNNNNGC 5 cut(s) 164, 338, 987, 1046, 1055
HpyF3I CTNAG 6 cut(s) 163, 264, 287, 590, 759, 847
HpySE526I ACGT 2 cut(s) 830, 834
Hsp92II CATG 2 cut(s) 329, 1137
HspAI GCGC 1 cut(s) 226
Kzo9I GATC 3 cut(s) 526, 803, 895
LmnI GCTCC 2 cut(s) 632, 816
Lsp1109I GCAGC 8 cut(s) 38, 39, 154, 719, 787, 833, 899, 1002
LweI GCATC 3 cut(s) 549, 662, 862
MaeI CTAG 2 cut(s) 474, 636
MaeII ACGT 2 cut(s) 830, 834
MaeIII GTNAC 1 cut(s) 920
MalI GATC 3 cut(s) 528, 805, 897
MbiI CCGCTC 1 cut(s) 729
MboI GATC 3 cut(s) 526, 803, 895
MboII GAAGA 2 cut(s) 477, 572
MluCI AATT 3 cut(s) 316, 334, 399
MlyI GAGTC 2 cut(s) 131, 296
MmeI TCCRAC 2 cut(s) 568, 1022
MnlI CCTC 7 cut(s) 195, 198, 475, 583, 675, 753, 825
MroXI GAANNNNTTC 1 cut(s) 217
MseI TTAA 1 cut(s) 780
MspA1I CMGCKG 2 cut(s) 29, 167
MspCI CTTAAG 1 cut(s) 779
MspI CCGG 7 cut(s) 99, 222, 345, 365, 479, 772, 1094
MspR9I CCNGG 8 cut(s) 13, 100, 223, 299, 366, 711, 1037, 1094
Mva1269I GAATGC 3 cut(s) 393, 824, 1065
MvaI CCWGG 4 cut(s) 13, 299, 711, 1037
MwoI GCNNNNNNNGC 5 cut(s) 164, 338, 987, 1046, 1055
NciI CCSGG 4 cut(s) 100, 223, 366, 1094
NdeII GATC 3 cut(s) 526, 803, 895
NlaIII CATG 2 cut(s) 329, 1137
NlaIV GGNNCC 1 cut(s) 97
PctI GAATGC 3 cut(s) 393, 824, 1065
PdmI GAANNNNTTC 1 cut(s) 217
PfeI GAWTC 2 cut(s) 417, 930
Pfl23II CGTACG 1 cut(s) 831
PfoI TCCNGGA 1 cut(s) 98
PinAI ACCGGT 1 cut(s) 344
PleI GAGTC 2 cut(s) 131, 296
PpsI GAGTC 2 cut(s) 131, 296
Ppu21I YACGTR 1 cut(s) 831
PsiI TTATAA 1 cut(s) 248
Psp6I CCWGG 4 cut(s) 11, 297, 709, 1035
PspFI CCCAGC 1 cut(s) 55
PspGI CCWGG 4 cut(s) 11, 297, 709, 1035
PspLI CGTACG 1 cut(s) 831
PspN4I GGNNCC 1 cut(s) 97
PspPI GGNCC 1 cut(s) 662
PvuII CAGCTG 2 cut(s) 29, 167
RsaI GTAC 2 cut(s) 833, 845
RsaNI GTAC 2 cut(s) 832, 844
SaqAI TTAA 1 cut(s) 780
Sau3AI GATC 3 cut(s) 526, 803, 895
Sau96I GGNCC 1 cut(s) 662
SchI GAGTC 2 cut(s) 131, 296
ScrFI CCNGG 8 cut(s) 13, 100, 223, 299, 366, 711, 1037, 1094
SfaNI GCATC 3 cut(s) 549, 662, 862
SfcI CTRYAG 1 cut(s) 682
SmlI CTYRAG 2 cut(s) 666, 779
SmoI CTYRAG 2 cut(s) 666, 779
Sse9I AATT 3 cut(s) 316, 334, 399
SsiI CCGC 9 cut(s) 60, 71, 93, 369, 729, 752, 915, 945, 1041
SspMI CTAG 2 cut(s) 474, 636
StyD4I CCNGG 8 cut(s) 11, 98, 221, 297, 364, 709, 1035, 1092
StyI CCWWGG 1 cut(s) 473
TaaI ACNGT 1 cut(s) 973
TaiI ACGT 2 cut(s) 833, 837
TaqI TCGA 5 cut(s) 252, 510, 704, 837, 903
TasI AATT 3 cut(s) 316, 334, 399
TatI WGTACW 1 cut(s) 843
TauI GCSGC 3 cut(s) 732, 918, 1043
TfiI GAWTC 2 cut(s) 417, 930
Tru1I TTAA 1 cut(s) 780
Tru9I TTAA 1 cut(s) 780
TscAI CASTG 3 cut(s) 157, 813, 1056
TseI GCWGC 8 cut(s) 26, 52, 167, 732, 775, 821, 912, 990
TspDTI ATGAA 4 cut(s) 342, 374, 400, 443
TspGWI ACGGA 2 cut(s) 369, 942
TspRI CASTG 3 cut(s) 157, 813, 1056
Vha464I CTTAAG 1 cut(s) 779
XapI RAATTY 2 cut(s) 334, 399
XmaJI CCTAGG 1 cut(s) 473
XmnI GAANNNNTTC 1 cut(s) 217
XspI CTAG 2 cut(s) 474, 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.