FvH4_6g50740

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0010447
Physical Location & Seq
Reverse (-)
37709533 .. 37711667
2135 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g50740.t2

Sequence Viewer

Length: 1212 bp
ATGGAGGCAGATAGTGGACATGAGTCGGTTTCTCTTTGTACACTTGATAAACTCCAAACGCTGCTGTCTCAAAGTTTAAAGTACCACTTCATAGTTGATAAGTCTAAGCTTATCATGGATCTTGTGAGGCTACTCTTTGCACTACTTGCCTTTCTAGCTTTGGGAGTGAATGCTGGTGGTTCTGGGAGTTGTAGGTTTCCAGCCATATACAACTTCGGCGACTCAAACTCTGATACCGGTGCTATTTCAGCTGCCATAGCTGAGGTTCCTCCTCCCAATGGTGAAAGTTTCTTTGGACATTCTTCTGGACGCTTTTGTGATGGTCGCCTTATAATAGATTTCATAGCTGAGAAGTTAAAGCTACCATATCTCAGTCCATACTTGGACTCAGTTGGGACTAATTTCAGACATGGTGCGAATTTTGCAACCGGCGGTTCATCCATTAGGCCAGGCGGTTACAGCCCTTTTCATCTCGGCATTCAACTTTCACAATTCATAAGATTCAAGTCTCAATCCATAGCCCTCTACAAGCAACAAAGTCCAGATACTCAAAGACTTCCTAGGCCGGAGGACTTCTCGAAAGCTCTATACACGATTGATATTGGTCAGAATGATCTTGCCTATGGTTTTCAACACGGGACAATAGAGCAAGTTCGAGCCTCCATTCCTGATATTCTCTCTCAGTTAGACCAAGCCATCAATAAACTATATAAAGAAGGGGCAAGGTTTTTTTGGGTGCATAACACAGGCCCTCATGGTTGCTTACCCTATAATGTTATTTACGACAAATCAAAGCCTGGAAATCTGGACAAGAGTGGTTGTGTGAAGCCTACCAACGAGGTGGCTCAGGAGTTCAATCGGCGGCTTAAGAACAGCGTATCACACTTGAGGAAGCAGCTCCCTCTTGCAGTATTCACTTATGTTGATGTGTATTCAGCTAAATATACAGTAATCAGCAAGGCCAAAAATGAAGGTTTTGTGGATCCATTCGACTTCTGCTGTGGCAGTTACAAGGGCTATCATATTGATTGTGGAAAGAAGGCCATAGTGAATGGAACAGTATATGGCAACCCTTGTCATAATCCATCAAAACATATTAGTTGGGATGGTATACACTACTCTCAGGCAGCAAACATATTGCTTGCAAATCAAATTATCAACGGCTTACTATCTGATCCACCGGTTTCAATCACAGAGGCTTGTCATGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

404

Amino Acids

44.26

Weight (kDa)

8.23

Isoelectric Point (pI)

35.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 69 - 354 9e-36 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 332
AccI GTMKAC 1 cut(s) 1111
AciI CCGC 3 cut(s) 432, 453, 862
AclWI GGATC 4 cut(s) 126, 977, 990, 1169
AcsI RAATTY 1 cut(s) 418
AfaI GTAC 2 cut(s) 40, 83
AfiI CCNNNNNNNGG 1 cut(s) 278
AflII CTTAAG 1 cut(s) 866
AgeI ACCGGT 2 cut(s) 236, 1180
AgsI TTSAA 5 cut(s) 482, 505, 632, 856, 1188
AjnI CCWGG 2 cut(s) 448, 796
AjuI GAANNNNNNNTTGG 2 cut(s) 276, 308
AluBI AGCT 9 cut(s) 109, 158, 251, 260, 347, 361, 584, 898, 938
AluI AGCT 9 cut(s) 109, 158, 251, 260, 347, 361, 584, 898, 938
Alw26I GTCTC 2 cut(s) 72, 513
AlwI GGATC 4 cut(s) 126, 977, 990, 1169
AoxI GGCC 5 cut(s) 446, 563, 748, 960, 1041
ApeKI GCWGC 4 cut(s) 61, 251, 895, 1127
ApoI RAATTY 1 cut(s) 418
AsiGI ACCGGT 2 cut(s) 236, 1180
AspA2I CCTAGG 1 cut(s) 560
AspS9I GGNCC 1 cut(s) 749
AsuHPI GGTGA 1 cut(s) 293
AvrII CCTAGG 1 cut(s) 560
BamHI GGATCC 1 cut(s) 982
BbvCI CCTCAGC 1 cut(s) 261
BbvI GCAGC 4 cut(s) 48, 238, 907, 1139
BccI CCATC 4 cut(s) 314, 704, 1093, 1100
BceAI ACGGC 1 cut(s) 1177
BciT130I CCWGG 2 cut(s) 450, 798
BcoDI GTCTC 2 cut(s) 72, 513
BfaI CTAG 2 cut(s) 155, 561
BfrI CTTAAG 1 cut(s) 866
BisI GCNGC 5 cut(s) 62, 252, 863, 896, 1128
BlnI CCTAGG 1 cut(s) 560
BlsI GCNGC 5 cut(s) 63, 253, 864, 897, 1129
Bme1390I CCNGG 2 cut(s) 450, 798
BmgT120I GGNCC 1 cut(s) 749
BmiI GGNNCC 2 cut(s) 267, 984
BmrFI CCNGG 2 cut(s) 450, 798
BoxI GACNNNNGTC 1 cut(s) 22
BplI GAGNNNNNCTC 2 cut(s) 560, 592
Bpu10I CCTNAGC 2 cut(s) 261, 846
BpuEI CTTGAG 1 cut(s) 907
BsaJI CCNNGG 1 cut(s) 560
BsaWI WCCGGW 2 cut(s) 236, 1180
Bsc4I CCNNNNNNNGG 1 cut(s) 278
Bse118I RCCGGY 3 cut(s) 236, 428, 1180
BseBI CCWGG 2 cut(s) 450, 798
BseDI CCNNGG 1 cut(s) 560
BseGI GGATG 2 cut(s) 437, 1111
BseLI CCNNNNNNNGG 1 cut(s) 278
BseMII CTCAG 7 cut(s) 252, 339, 385, 402, 695, 860, 1136
BseRI GAGGAG 1 cut(s) 261
BseXI GCAGC 4 cut(s) 48, 238, 907, 1139
BshFI GGCC 5 cut(s) 448, 565, 750, 962, 1043
BshTI ACCGGT 2 cut(s) 236, 1180
BsiSI CCGG 4 cut(s) 237, 429, 566, 1181
BslFI GGGAC 2 cut(s) 409, 652
BslI CCNNNNNNNGG 1 cut(s) 278
BsmAI GTCTC 2 cut(s) 72, 513
BsmFI GGGAC 2 cut(s) 409, 652
BsmI GAATGC 2 cut(s) 175, 477
BsnI GGCC 5 cut(s) 448, 565, 750, 962, 1043
Bsp1407I TGTACA 1 cut(s) 38
Bsp143I GATC 4 cut(s) 118, 613, 982, 1174
BspACI CCGC 3 cut(s) 432, 453, 862
BspANI GGCC 5 cut(s) 448, 565, 750, 962, 1043
BspCNI CTCAG 7 cut(s) 253, 340, 384, 401, 694, 859, 1135
BspLI GGNNCC 2 cut(s) 267, 984
BspPI GGATC 4 cut(s) 126, 977, 990, 1169
BspTI CTTAAG 1 cut(s) 866
BsrFI RCCGGY 3 cut(s) 236, 428, 1180
BsrGI TGTACA 1 cut(s) 38
BssAI RCCGGY 3 cut(s) 236, 428, 1180
BssECI CCNNGG 1 cut(s) 560
BssMI GATC 4 cut(s) 118, 613, 982, 1174
BssNAI GTATAC 1 cut(s) 1112
BssT1I CCWWGG 1 cut(s) 560
Bst1107I GTATAC 1 cut(s) 1112
Bst2UI CCWGG 2 cut(s) 450, 798
Bst4CI ACNGT 2 cut(s) 949, 1060
BstAFI CTTAAG 1 cut(s) 866
BstAPI GCANNNNNTGC 1 cut(s) 146
BstAUI TGTACA 1 cut(s) 38
BstC8I GCNNGC 1 cut(s) 1143
BstDEI CTNAG 8 cut(s) 105, 261, 348, 371, 388, 681, 846, 1122
BstF5I GGATG 2 cut(s) 437, 1111
BstKTI GATC 4 cut(s) 121, 616, 985, 1177
BstMAI GTCTC 2 cut(s) 72, 513
BstMBI GATC 4 cut(s) 118, 613, 982, 1174
BstMWI GCNNNNNNNGC 6 cut(s) 146, 155, 248, 257, 422, 459
BstNI CCWGG 2 cut(s) 450, 798
BstPAI GACNNNNGTC 1 cut(s) 22
BstSCI CCNGG 2 cut(s) 448, 796
BstV1I GCAGC 4 cut(s) 48, 238, 907, 1139
BstX2I RGATCY 2 cut(s) 118, 982
BstXI CCANNNNNNTGG 1 cut(s) 841
BstYI RGATCY 2 cut(s) 118, 982
BstZ17I GTATAC 1 cut(s) 1112
BsuRI GGCC 5 cut(s) 448, 565, 750, 962, 1043
BtsCI GGATG 2 cut(s) 437, 1111
Cac8I GCNNGC 1 cut(s) 1143
Cfr10I RCCGGY 3 cut(s) 236, 428, 1180
Cfr13I GGNCC 1 cut(s) 749
CseI GACGC 1 cut(s) 318
Csp6I GTAC 2 cut(s) 39, 82
CspAI ACCGGT 2 cut(s) 236, 1180
CviAII CATG 5 cut(s) 20, 115, 410, 755, 1205
CviQI GTAC 2 cut(s) 39, 82
DdeI CTNAG 8 cut(s) 105, 261, 348, 371, 388, 681, 846, 1122
DpnI GATC 4 cut(s) 120, 615, 984, 1176
DpnII GATC 4 cut(s) 118, 613, 982, 1174
DraI TTTAAA 1 cut(s) 78
Eco130I CCWWGG 1 cut(s) 560
EcoO109I RGGNCCY 1 cut(s) 749
EcoRII CCWGG 2 cut(s) 448, 796
EcoT14I CCWWGG 1 cut(s) 560
ErhI CCWWGG 1 cut(s) 560
FaeI CATG 5 cut(s) 23, 118, 413, 758, 1208
FalI AAGNNNNNCTT 2 cut(s) 71, 103
FaqI GGGAC 2 cut(s) 409, 652
FatI CATG 5 cut(s) 19, 114, 409, 754, 1204
FblI GTMKAC 1 cut(s) 1111
Fnu4HI GCNGC 5 cut(s) 62, 252, 863, 896, 1128
FokI GGATG 2 cut(s) 424, 1118
Fsp4HI GCNGC 5 cut(s) 62, 252, 863, 896, 1128
FspBI CTAG 2 cut(s) 155, 561
GluI GCNGC 5 cut(s) 62, 252, 863, 896, 1128
HaeIII GGCC 5 cut(s) 448, 565, 750, 962, 1043
HapII CCGG 4 cut(s) 237, 429, 566, 1181
HgaI GACGC 1 cut(s) 318
Hin1II CATG 5 cut(s) 23, 118, 413, 758, 1208
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 4 cut(s) 23, 221, 386, 501
HpaII CCGG 4 cut(s) 237, 429, 566, 1181
HphI GGTGA 1 cut(s) 293
Hpy166II GTNNAC 3 cut(s) 17, 41, 1112
Hpy188I TCNGA 4 cut(s) 232, 407, 609, 1174
Hpy188III TCNNGA 6 cut(s) 306, 542, 577, 668, 806, 848
Hpy8I GTNNAC 3 cut(s) 17, 41, 1112
HpyAV CCTTC 3 cut(s) 710, 965, 1033
HpyCH4III ACNGT 2 cut(s) 949, 1060
HpyCH4V TGCA 5 cut(s) 140, 425, 739, 908, 1145
HpyF10VI GCNNNNNNNGC 6 cut(s) 146, 155, 248, 257, 422, 459
HpyF3I CTNAG 8 cut(s) 105, 261, 348, 371, 388, 681, 846, 1122
Hsp92II CATG 5 cut(s) 23, 118, 413, 758, 1208
Kzo9I GATC 4 cut(s) 118, 613, 982, 1174
LmnI GCTCC 1 cut(s) 903
Lsp1109I GCAGC 4 cut(s) 48, 238, 907, 1139
MaeI CTAG 2 cut(s) 155, 561
MaeIII GTNAC 2 cut(s) 455, 1007
MalI GATC 4 cut(s) 120, 615, 984, 1176
MboI GATC 4 cut(s) 118, 613, 982, 1174
MboII GAAGA 1 cut(s) 294
MflI RGATCY 2 cut(s) 118, 982
MluCI AATT 4 cut(s) 400, 418, 491, 1152
MlyI GAGTC 3 cut(s) 32, 215, 380
MseI TTAA 3 cut(s) 77, 356, 867
MspA1I CMGCKG 1 cut(s) 251
MspCI CTTAAG 1 cut(s) 866
MspI CCGG 4 cut(s) 237, 429, 566, 1181
MspR9I CCNGG 2 cut(s) 450, 798
Mva1269I GAATGC 2 cut(s) 175, 477
MvaI CCWGG 2 cut(s) 450, 798
MwoI GCNNNNNNNGC 6 cut(s) 146, 155, 248, 257, 422, 459
NdeII GATC 4 cut(s) 118, 613, 982, 1174
NlaIII CATG 5 cut(s) 23, 118, 413, 758, 1208
NlaIV GGNNCC 2 cut(s) 267, 984
NmeAIII GCCGAG 1 cut(s) 453
PctI GAATGC 2 cut(s) 175, 477
PfeI GAWTC 1 cut(s) 501
PinAI ACCGGT 2 cut(s) 236, 1180
PkrI GCNGC 5 cut(s) 63, 253, 864, 897, 1129
PleI GAGTC 3 cut(s) 31, 215, 380
PpsI GAGTC 3 cut(s) 31, 215, 380
PshAI GACNNNNGTC 1 cut(s) 22
PsiI TTATAA 1 cut(s) 332
Psp6I CCWGG 2 cut(s) 448, 796
PspGI CCWGG 2 cut(s) 448, 796
PspN4I GGNNCC 2 cut(s) 267, 984
PspPI GGNCC 1 cut(s) 749
PsuI RGATCY 2 cut(s) 118, 982
PvuII CAGCTG 1 cut(s) 251
RsaI GTAC 2 cut(s) 40, 83
RsaNI GTAC 2 cut(s) 39, 82
SaqAI TTAA 3 cut(s) 77, 356, 867
SatI GCNGC 5 cut(s) 62, 252, 863, 896, 1128
Sau3AI GATC 4 cut(s) 118, 613, 982, 1174
Sau96I GGNCC 1 cut(s) 749
SchI GAGTC 3 cut(s) 32, 215, 380
ScrFI CCNGG 2 cut(s) 450, 798
SmlI CTYRAG 2 cut(s) 866, 886
SmoI CTYRAG 2 cut(s) 866, 886
Sse9I AATT 4 cut(s) 400, 418, 491, 1152
SsiI CCGC 3 cut(s) 432, 453, 862
SspMI CTAG 2 cut(s) 155, 561
StyD4I CCNGG 2 cut(s) 448, 796
StyI CCWWGG 1 cut(s) 560
TaaI ACNGT 2 cut(s) 949, 1060
TaqI TCGA 3 cut(s) 578, 655, 990
TasI AATT 4 cut(s) 400, 418, 491, 1152
TatI WGTACW 1 cut(s) 38
TauI GCSGC 1 cut(s) 865
TfiI GAWTC 1 cut(s) 501
Tru1I TTAA 3 cut(s) 77, 356, 867
Tru9I TTAA 3 cut(s) 77, 356, 867
TseI GCWGC 4 cut(s) 61, 251, 895, 1127
TspDTI ATGAA 6 cut(s) 79, 331, 426, 458, 484, 984
Vha464I CTTAAG 1 cut(s) 866
XapI RAATTY 1 cut(s) 418
XmaJI CCTAGG 1 cut(s) 560
XmiI GTMKAC 1 cut(s) 1111
XspI CTAG 2 cut(s) 155, 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.