Rw2G052160

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0010447
Physical Location & Seq
Reverse (-)
79972773 .. 79974293
1521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G052160.1

Sequence Viewer

Length: 1098 bp
ATGGATCTTGTGAGGCTATTTTTTGCAGTACTTGGCATTTTAGCTTTGGGAGTGAATGCTGGTGGTTCTGGGAGTTGTAGGTTTCCGGCCATATACAACTTTGGTGACTCAAACTCCGACACCGGCGCTATTTCAGCCGCCATAGAAGTGGTTCCTCCTCCCAATGGTGAAAGTTTCTTTGGACATTCTTCTGGACGCTTTTCTGATGGTCGCCTTATTATAGATTTCATAGCTGAGAAGTTGCAGCTACCATATCTCAGTCCATACTTGGACTCACTTGGGACTAATTTCAGGCATGGTGCAAATTTTGCAACTGGCGGTTCATCCATTAGGCCAGGCGGTTATAGCCCGTTTCATCTCGGCATTCAAGTTTCACAGTTCGTAAGATTCAAGTCTCAATCCATAGCCCTCTACAAGCAACAAAGTTCAAACACTCAAAGACTTCCTAGGCCGGAGGACTTCTCGAAAGCTTTGTACACAATTGATATTGGTCAGAATGATCTTGCCTATGGTTTTCAACACGGGACGATTCAGCAAGTTCAAGCTTCCATTCCTGATATTCTCACTCAGTTAGACCAAGCCATGAATAAACTATACAAAGAAGGGGCAAGGTTTTTTTGGGTGCATAACACAGGCCCTCATGGGTGCTTGCCCTATAATGTCATCTATGACAAATCAAAGCCTGGTAATCTGGACCAGATTGGCTGCGTGAAGCCTACCAACGAGGTAGCTCAGGAGTTCAATCGGCAGCTTAAGAACAGCGTATTAAACTTGAGGAAGCAGCTCCCTCTTGCAGTATTCACTTATGTCGATGTGTATTCAGCTAAATATACAGTGATAAGCAACGCGAAAAATGAAGGTTTTGTGGATCCATTTGACTTCTGCTGTGGCAGTTACAAGGGGTACCATATTGATTGTGGAAAGAAGGCCATAGTGAATGGAACGGTATATGGAAACCCTTGCAATAATCCATCAAGACATATTAGTTGGGATGGCATACACTACTCTCAGGCAGCAAACATATTGCTTGCCAATCAAATTATTAACGGCTCACTATCTGATCCACCAGTTTCAATCACAGAGGCTTGTCATGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

365

Amino Acids

39.93

Weight (kDa)

7.6

Isoelectric Point (pI)

33.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 31 - 347 3e-46 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 903
AccB1I GGYRCC 1 cut(s) 903
AccII CGCG 1 cut(s) 848
AciI CCGC 3 cut(s) 138, 318, 339
AclWI GGATC 4 cut(s) 12, 863, 876, 1055
AcoI YGGCCR 1 cut(s) 87
AcsI RAATTY 1 cut(s) 304
AfaI GTAC 3 cut(s) 30, 476, 905
AfiI CCNNNNNNNGG 1 cut(s) 164
AflII CTTAAG 1 cut(s) 752
AgsI TTSAA 7 cut(s) 368, 391, 429, 518, 542, 742, 1074
AjnI CCWGG 2 cut(s) 334, 682
AjuI GAANNNNNNNTTGG 2 cut(s) 162, 194
AluBI AGCT 9 cut(s) 44, 233, 247, 470, 545, 731, 751, 784, 824
AluI AGCT 9 cut(s) 44, 233, 247, 470, 545, 731, 751, 784, 824
Alw26I GTCTC 1 cut(s) 399
AlwI GGATC 4 cut(s) 12, 863, 876, 1055
AoxI GGCC 5 cut(s) 87, 332, 449, 634, 927
ApeKI GCWGC 5 cut(s) 244, 705, 748, 781, 1013
ApoI RAATTY 1 cut(s) 304
Asp700I GAANNNNTTC 1 cut(s) 150
Asp718I GGTACC 1 cut(s) 903
AspA2I CCTAGG 1 cut(s) 446
AspLEI GCGC 1 cut(s) 128
AspS9I GGNCC 2 cut(s) 635, 694
AsuHPI GGTGA 2 cut(s) 116, 179
AvaII GGWCC 1 cut(s) 694
AvrII CCTAGG 1 cut(s) 446
BamHI GGATCC 1 cut(s) 868
BanI GGYRCC 1 cut(s) 903
BbvI GCAGC 5 cut(s) 256, 692, 760, 793, 1025
BccI CCATC 3 cut(s) 200, 979, 986
BceAI ACGGC 1 cut(s) 1063
BciT130I CCWGG 2 cut(s) 336, 684
BcoDI GTCTC 1 cut(s) 399
BfaI CTAG 1 cut(s) 447
BfoI RGCGCY 1 cut(s) 129
BfrI CTTAAG 1 cut(s) 752
BisI GCNGC 6 cut(s) 138, 245, 706, 749, 782, 1014
BlnI CCTAGG 1 cut(s) 446
BlsI GCNGC 6 cut(s) 139, 246, 707, 750, 783, 1015
BmcAI AGTACT 1 cut(s) 30
Bme1390I CCNGG 2 cut(s) 336, 684
Bme18I GGWCC 1 cut(s) 694
BmgT120I GGNCC 2 cut(s) 635, 694
BmiI GGNNCC 3 cut(s) 153, 870, 905
BmrFI CCNGG 2 cut(s) 336, 684
BplI GAGNNNNNCTC 2 cut(s) 446, 478
Bpu10I CCTNAGC 1 cut(s) 732
BpuEI CTTGAG 1 cut(s) 793
BsaJI CCNNGG 1 cut(s) 446
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 1 cut(s) 164
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 2 cut(s) 319, 1067
BseBI CCWGG 2 cut(s) 336, 684
BseDI CCNNGG 1 cut(s) 446
BseGI GGATG 2 cut(s) 323, 997
BseLI CCNNNNNNNGG 1 cut(s) 164
BseMII CTCAG 5 cut(s) 225, 271, 581, 746, 1022
BseNI ACTGG 2 cut(s) 319, 1067
BseRI GAGGAG 1 cut(s) 147
BseXI GCAGC 5 cut(s) 256, 692, 760, 793, 1025
Bsh1236I CGCG 1 cut(s) 848
BshFI GGCC 5 cut(s) 89, 334, 451, 636, 929
BshNI GGYRCC 1 cut(s) 903
BsiSI CCGG 3 cut(s) 86, 123, 452
BslFI GGGAC 2 cut(s) 295, 538
BslI CCNNNNNNNGG 1 cut(s) 164
BsmAI GTCTC 1 cut(s) 399
BsmFI GGGAC 2 cut(s) 295, 538
BsmI GAATGC 2 cut(s) 61, 363
BsnI GGCC 5 cut(s) 89, 334, 451, 636, 929
Bsp1407I TGTACA 1 cut(s) 474
Bsp143I GATC 4 cut(s) 4, 499, 868, 1060
BspACI CCGC 3 cut(s) 138, 318, 339
BspANI GGCC 5 cut(s) 89, 334, 451, 636, 929
BspCNI CTCAG 5 cut(s) 226, 270, 580, 745, 1021
BspFNI CGCG 1 cut(s) 848
BspLI GGNNCC 3 cut(s) 153, 870, 905
BspPI GGATC 4 cut(s) 12, 863, 876, 1055
BspT107I GGYRCC 1 cut(s) 903
BspTI CTTAAG 1 cut(s) 752
BsrFI RCCGGY 1 cut(s) 122
BsrGI TGTACA 1 cut(s) 474
BsrI ACTGG 2 cut(s) 319, 1067
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 1 cut(s) 446
BssMI GATC 4 cut(s) 4, 499, 868, 1060
BssT1I CCWWGG 1 cut(s) 446
Bst2UI CCWGG 2 cut(s) 336, 684
Bst4CI ACNGT 3 cut(s) 378, 835, 946
BstAFI CTTAAG 1 cut(s) 752
BstAPI GCANNNNNTGC 1 cut(s) 308
BstAUI TGTACA 1 cut(s) 474
BstC8I GCNNGC 2 cut(s) 650, 1029
BstDEI CTNAG 5 cut(s) 234, 257, 567, 732, 1008
BstF5I GGATG 2 cut(s) 323, 997
BstFNI CGCG 1 cut(s) 848
BstH2I RGCGCY 1 cut(s) 129
BstHHI GCGC 1 cut(s) 128
BstKTI GATC 4 cut(s) 7, 502, 871, 1063
BstMAI GTCTC 1 cut(s) 399
BstMBI GATC 4 cut(s) 4, 499, 868, 1060
BstMWI GCNNNNNNNGC 3 cut(s) 134, 308, 345
BstNI CCWGG 2 cut(s) 336, 684
BstSCI CCNGG 2 cut(s) 334, 682
BstUI CGCG 1 cut(s) 848
BstV1I GCAGC 5 cut(s) 256, 692, 760, 793, 1025
BstX2I RGATCY 2 cut(s) 4, 868
BstXI CCANNNNNNTGG 1 cut(s) 148
BstYI RGATCY 2 cut(s) 4, 868
BsuRI GGCC 5 cut(s) 89, 334, 451, 636, 929
BtsCI GGATG 2 cut(s) 323, 997
BtsIMutI CAGTG 1 cut(s) 840
Cac8I GCNNGC 2 cut(s) 650, 1029
CfoI GCGC 1 cut(s) 128
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 2 cut(s) 635, 694
CseI GACGC 1 cut(s) 204
Csp6I GTAC 3 cut(s) 29, 475, 904
CviAII CATG 4 cut(s) 296, 583, 641, 1091
CviQI GTAC 3 cut(s) 29, 475, 904
DdeI CTNAG 5 cut(s) 234, 257, 567, 732, 1008
DpnI GATC 4 cut(s) 6, 501, 870, 1062
DpnII GATC 4 cut(s) 4, 499, 868, 1060
EaeI YGGCCR 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 446
Eco47I GGWCC 1 cut(s) 694
EcoO109I RGGNCCY 1 cut(s) 635
EcoRII CCWGG 2 cut(s) 334, 682
EcoT14I CCWWGG 1 cut(s) 446
ErhI CCWWGG 1 cut(s) 446
FaeI CATG 4 cut(s) 299, 586, 644, 1094
FaqI GGGAC 2 cut(s) 295, 538
FatI CATG 4 cut(s) 295, 582, 640, 1090
Fnu4HI GCNGC 6 cut(s) 138, 245, 706, 749, 782, 1014
FokI GGATG 2 cut(s) 310, 1004
Fsp4HI GCNGC 6 cut(s) 138, 245, 706, 749, 782, 1014
FspBI CTAG 1 cut(s) 447
GlaI GCGC 1 cut(s) 127
GluI GCNGC 6 cut(s) 138, 245, 706, 749, 782, 1014
HaeII RGCGCY 1 cut(s) 129
HaeIII GGCC 5 cut(s) 89, 334, 451, 636, 929
HapII CCGG 3 cut(s) 86, 123, 452
HgaI GACGC 1 cut(s) 204
HhaI GCGC 1 cut(s) 128
Hin1II CATG 4 cut(s) 299, 586, 644, 1094
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HindIII AAGCTT 2 cut(s) 468, 543
HinfI GANTC 4 cut(s) 107, 272, 387, 529
HpaII CCGG 3 cut(s) 86, 123, 452
HphI GGTGA 2 cut(s) 116, 179
Hpy166II GTNNAC 1 cut(s) 477
Hpy188I TCNGA 4 cut(s) 118, 205, 495, 1060
Hpy188III TCNNGA 6 cut(s) 192, 463, 554, 692, 734, 975
Hpy8I GTNNAC 1 cut(s) 477
HpyAV CCTTC 3 cut(s) 596, 851, 919
HpyCH4III ACNGT 3 cut(s) 378, 835, 946
HpyCH4V TGCA 7 cut(s) 26, 244, 302, 311, 625, 794, 963
HpyF10VI GCNNNNNNNGC 3 cut(s) 134, 308, 345
HpyF3I CTNAG 5 cut(s) 234, 257, 567, 732, 1008
Hsp92II CATG 4 cut(s) 299, 586, 644, 1094
HspAI GCGC 1 cut(s) 126
KpnI GGTACC 1 cut(s) 907
Kzo9I GATC 4 cut(s) 4, 499, 868, 1060
LmnI GCTCC 1 cut(s) 789
Lsp1109I GCAGC 5 cut(s) 256, 692, 760, 793, 1025
MaeI CTAG 1 cut(s) 447
MaeIII GTNAC 2 cut(s) 104, 893
MalI GATC 4 cut(s) 6, 501, 870, 1062
MboI GATC 4 cut(s) 4, 499, 868, 1060
MboII GAAGA 1 cut(s) 180
MfeI CAATTG 1 cut(s) 480
MflI RGATCY 2 cut(s) 4, 868
MluCI AATT 4 cut(s) 286, 304, 480, 1038
MlyI GAGTC 2 cut(s) 101, 266
MmeI TCCRAC 1 cut(s) 141
MroXI GAANNNNTTC 1 cut(s) 150
MseI TTAA 3 cut(s) 753, 767, 1044
MslI CAYNNNNRTG 1 cut(s) 146
MspCI CTTAAG 1 cut(s) 752
MspI CCGG 3 cut(s) 86, 123, 452
MspR9I CCNGG 2 cut(s) 336, 684
MunI CAATTG 1 cut(s) 480
Mva1269I GAATGC 2 cut(s) 61, 363
MvaI CCWGG 2 cut(s) 336, 684
MvnI CGCG 1 cut(s) 848
MwoI GCNNNNNNNGC 3 cut(s) 134, 308, 345
NdeII GATC 4 cut(s) 4, 499, 868, 1060
NlaIII CATG 4 cut(s) 299, 586, 644, 1094
NlaIV GGNNCC 3 cut(s) 153, 870, 905
NmeAIII GCCGAG 1 cut(s) 339
NmuCI GTSAC 1 cut(s) 104
PctI GAATGC 2 cut(s) 61, 363
PdmI GAANNNNTTC 1 cut(s) 150
PfeI GAWTC 2 cut(s) 387, 529
PkrI GCNGC 6 cut(s) 139, 246, 707, 750, 783, 1015
PleI GAGTC 2 cut(s) 101, 266
PpsI GAGTC 2 cut(s) 101, 266
Psp6I CCWGG 2 cut(s) 334, 682
PspGI CCWGG 2 cut(s) 334, 682
PspN4I GGNNCC 3 cut(s) 153, 870, 905
PspPI GGNCC 2 cut(s) 635, 694
PsuI RGATCY 2 cut(s) 4, 868
RsaI GTAC 3 cut(s) 30, 476, 905
RsaNI GTAC 3 cut(s) 29, 475, 904
RseI CAYNNNNRTG 1 cut(s) 146
SaqAI TTAA 3 cut(s) 753, 767, 1044
SatI GCNGC 6 cut(s) 138, 245, 706, 749, 782, 1014
Sau3AI GATC 4 cut(s) 4, 499, 868, 1060
Sau96I GGNCC 2 cut(s) 635, 694
ScaI AGTACT 1 cut(s) 30
SchI GAGTC 2 cut(s) 101, 266
ScrFI CCNGG 2 cut(s) 336, 684
SgrAI CRCCGGYG 1 cut(s) 122
SinI GGWCC 1 cut(s) 694
SmiMI CAYNNNNRTG 1 cut(s) 146
SmlI CTYRAG 2 cut(s) 752, 772
SmoI CTYRAG 2 cut(s) 752, 772
Sse9I AATT 4 cut(s) 286, 304, 480, 1038
SsiI CCGC 3 cut(s) 138, 318, 339
SspMI CTAG 1 cut(s) 447
StyD4I CCNGG 2 cut(s) 334, 682
StyI CCWWGG 1 cut(s) 446
TaaI ACNGT 3 cut(s) 378, 835, 946
TaqI TCGA 2 cut(s) 464, 810
TasI AATT 4 cut(s) 286, 304, 480, 1038
TatI WGTACW 2 cut(s) 28, 474
TauI GCSGC 1 cut(s) 140
TfiI GAWTC 2 cut(s) 387, 529
Tru1I TTAA 3 cut(s) 753, 767, 1044
Tru9I TTAA 3 cut(s) 753, 767, 1044
TscAI CASTG 1 cut(s) 840
TseFI GTSAC 1 cut(s) 104
TseI GCWGC 5 cut(s) 244, 705, 748, 781, 1013
Tsp45I GTSAC 1 cut(s) 104
TspDTI ATGAA 5 cut(s) 217, 312, 344, 599, 870
TspRI CASTG 1 cut(s) 840
Vha464I CTTAAG 1 cut(s) 752
VpaK11BI GGWCC 1 cut(s) 694
XapI RAATTY 1 cut(s) 304
XcmI CCANNNNNNNNNTGG 1 cut(s) 914
XmaJI CCTAGG 1 cut(s) 446
XmnI GAANNNNTTC 1 cut(s) 150
XspI CTAG 1 cut(s) 447
ZrmI AGTACT 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.