Rh2BG643300

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0010447
Physical Location & Seq
Reverse (-)
86197647 .. 86199167
1521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG643300.1

Sequence Viewer

Length: 957 bp
ATGGATCTTGTGAGGCTATTTTTTGCAGTACTTGGCATTTTAGCTTTGGGAGTGAATGCTGGTGGTTCTGGGAGTTGTAGGTTTCCGGCCATATACAACTTTGGTGACTCAAACTCCGACACCGGCGCTATTTCAGCCGCCATAGAAGTGGTTCCTCCTCCCAATGGTGAAAGTTTCTTTGGACATTCTTCTGGACGCTTTTCTGATGGTCGCCTTATTATAGATTTCATAGGTGAGCTATTATGCTGCACACTCACTCATTCAAATGAGAATGATGTAACTTTGGCACCAGCTCAAAGACTTCCTAGGCCGGAGGACTTCTCGAAAGCTTTGTACACAATTGATATTGGTCAGAATGATCTTGCCTATGGTTTTCAACATGGGACGATTCAGCAAGTTCAAGCTTCCATTCCTGATATTCTCACTCAGTTAGACCAAGCCATGAATAAACTATACAAAGAAGGGGCAAGGTTTTTTTGGGTGCATAACACAGGCCCTCATGGGTGCTTGCCCTATAATGTCATCTATGACAAATCAAAGCCTGGTAATCTGGACCAGATTGGCTGCGTGAAGCCTACCAACGAGGTAGCTCAGGAGTTCAATCGGCAGCTTAAGAACAGCGTATTAAACTTGAGGAAGCAGCTCCCTCTTGCAGTATTCACTTATGTCGATGTGTATTCAGCTAAATATACAGTGATAAGCAACGCGAAAAATGAAGGTTTTGTGGATCCATTCGACTTCTGCTGTGGCAGTTACAAGGGGTACCATATTGATTGTGGAAAGAAGGCCATAGTGAATGGAACGGTATATGGCAACCCTTGTAATAATCCATCAAGACATATTAGTTGGGATGGCATACACTACTCTCAGGCAGCAAACATATTGCTTGCCAATCAAATTATTAACGGCTCACTATCTGATCCACCAGTTTCAATCACAGAGGCTTGTCATGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

318

Amino Acids

34.66

Weight (kDa)

5.82

Isoelectric Point (pI)

29.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 31 - 300 6.2e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 762
AccB1I GGYRCC 2 cut(s) 286, 762
AccII CGCG 1 cut(s) 707
AciI CCGC 1 cut(s) 138
AclWI GGATC 4 cut(s) 12, 722, 735, 914
AcoI YGGCCR 1 cut(s) 87
AfaI GTAC 3 cut(s) 30, 335, 764
AfiI CCNNNNNNNGG 1 cut(s) 164
AflII CTTAAG 1 cut(s) 611
AgsI TTSAA 5 cut(s) 264, 377, 401, 601, 933
AjnI CCWGG 1 cut(s) 541
AjuI GAANNNNNNNTTGG 2 cut(s) 162, 194
AluBI AGCT 9 cut(s) 44, 238, 293, 329, 404, 590, 610, 643, 683
AluI AGCT 9 cut(s) 44, 238, 293, 329, 404, 590, 610, 643, 683
AlwI GGATC 4 cut(s) 12, 722, 735, 914
AoxI GGCC 4 cut(s) 87, 308, 493, 786
ApeKI GCWGC 5 cut(s) 246, 564, 607, 640, 872
Asp700I GAANNNNTTC 1 cut(s) 150
Asp718I GGTACC 1 cut(s) 762
AspA2I CCTAGG 1 cut(s) 305
AspLEI GCGC 1 cut(s) 128
AspS9I GGNCC 2 cut(s) 494, 553
AsuHPI GGTGA 3 cut(s) 116, 179, 245
AvaII GGWCC 1 cut(s) 553
AvrII CCTAGG 1 cut(s) 305
BamHI GGATCC 1 cut(s) 727
BanI GGYRCC 2 cut(s) 286, 762
BbvI GCAGC 5 cut(s) 233, 551, 619, 652, 884
BccI CCATC 3 cut(s) 200, 838, 845
BceAI ACGGC 1 cut(s) 922
BciT130I CCWGG 1 cut(s) 543
BfaI CTAG 1 cut(s) 306
BfoI RGCGCY 1 cut(s) 129
BfrI CTTAAG 1 cut(s) 611
BisI GCNGC 6 cut(s) 138, 247, 565, 608, 641, 873
BlnI CCTAGG 1 cut(s) 305
BlsI GCNGC 6 cut(s) 139, 248, 566, 609, 642, 874
BmcAI AGTACT 1 cut(s) 30
Bme1390I CCNGG 1 cut(s) 543
Bme18I GGWCC 1 cut(s) 553
BmgT120I GGNCC 2 cut(s) 494, 553
BmiI GGNNCC 4 cut(s) 153, 288, 729, 764
BmrFI CCNGG 1 cut(s) 543
BplI GAGNNNNNCTC 2 cut(s) 305, 337
Bpu10I CCTNAGC 1 cut(s) 591
BpuEI CTTGAG 1 cut(s) 652
BsaJI CCNNGG 1 cut(s) 305
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 1 cut(s) 164
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 1 cut(s) 926
BseBI CCWGG 1 cut(s) 543
BseDI CCNNGG 1 cut(s) 305
BseGI GGATG 1 cut(s) 856
BseLI CCNNNNNNNGG 1 cut(s) 164
BseMII CTCAG 3 cut(s) 440, 605, 881
BseNI ACTGG 1 cut(s) 926
BseRI GAGGAG 1 cut(s) 147
BseXI GCAGC 5 cut(s) 233, 551, 619, 652, 884
BsgI GTGCAG 1 cut(s) 232
Bsh1236I CGCG 1 cut(s) 707
BshFI GGCC 4 cut(s) 89, 310, 495, 788
BshNI GGYRCC 2 cut(s) 286, 762
BsiSI CCGG 3 cut(s) 86, 123, 311
BslFI GGGAC 1 cut(s) 397
BslI CCNNNNNNNGG 1 cut(s) 164
BsmFI GGGAC 1 cut(s) 397
BsmI GAATGC 1 cut(s) 61
BsnI GGCC 4 cut(s) 89, 310, 495, 788
Bsp1407I TGTACA 1 cut(s) 333
Bsp143I GATC 4 cut(s) 4, 358, 727, 919
BspACI CCGC 1 cut(s) 138
BspANI GGCC 4 cut(s) 89, 310, 495, 788
BspCNI CTCAG 3 cut(s) 439, 604, 880
BspFNI CGCG 1 cut(s) 707
BspLI GGNNCC 4 cut(s) 153, 288, 729, 764
BspPI GGATC 4 cut(s) 12, 722, 735, 914
BspT107I GGYRCC 2 cut(s) 286, 762
BspTI CTTAAG 1 cut(s) 611
BsrFI RCCGGY 1 cut(s) 122
BsrGI TGTACA 1 cut(s) 333
BsrI ACTGG 1 cut(s) 926
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 1 cut(s) 305
BssMI GATC 4 cut(s) 4, 358, 727, 919
BssT1I CCWWGG 1 cut(s) 305
Bst2UI CCWGG 1 cut(s) 543
Bst4CI ACNGT 2 cut(s) 694, 805
BstAFI CTTAAG 1 cut(s) 611
BstAUI TGTACA 1 cut(s) 333
BstC8I GCNNGC 2 cut(s) 509, 888
BstDEI CTNAG 3 cut(s) 426, 591, 867
BstF5I GGATG 1 cut(s) 856
BstFNI CGCG 1 cut(s) 707
BstH2I RGCGCY 1 cut(s) 129
BstHHI GCGC 1 cut(s) 128
BstKTI GATC 4 cut(s) 7, 361, 730, 922
BstMBI GATC 4 cut(s) 4, 358, 727, 919
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstNI CCWGG 1 cut(s) 543
BstSCI CCNGG 1 cut(s) 541
BstUI CGCG 1 cut(s) 707
BstV1I GCAGC 5 cut(s) 233, 551, 619, 652, 884
BstX2I RGATCY 2 cut(s) 4, 727
BstXI CCANNNNNNTGG 1 cut(s) 148
BstYI RGATCY 2 cut(s) 4, 727
BsuRI GGCC 4 cut(s) 89, 310, 495, 788
BtsCI GGATG 1 cut(s) 856
BtsIMutI CAGTG 1 cut(s) 699
Cac8I GCNNGC 2 cut(s) 509, 888
CfoI GCGC 1 cut(s) 128
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 2 cut(s) 494, 553
CseI GACGC 1 cut(s) 204
Csp6I GTAC 3 cut(s) 29, 334, 763
CviAII CATG 4 cut(s) 380, 442, 500, 950
CviQI GTAC 3 cut(s) 29, 334, 763
DdeI CTNAG 3 cut(s) 426, 591, 867
DpnI GATC 4 cut(s) 6, 360, 729, 921
DpnII GATC 4 cut(s) 4, 358, 727, 919
EaeI YGGCCR 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 305
Eco47I GGWCC 1 cut(s) 553
EcoO109I RGGNCCY 1 cut(s) 494
EcoRII CCWGG 1 cut(s) 541
EcoT14I CCWWGG 1 cut(s) 305
ErhI CCWWGG 1 cut(s) 305
FaeI CATG 4 cut(s) 383, 445, 503, 953
FaqI GGGAC 1 cut(s) 397
FatI CATG 4 cut(s) 379, 441, 499, 949
Fnu4HI GCNGC 6 cut(s) 138, 247, 565, 608, 641, 873
FokI GGATG 1 cut(s) 863
Fsp4HI GCNGC 6 cut(s) 138, 247, 565, 608, 641, 873
FspBI CTAG 1 cut(s) 306
GlaI GCGC 1 cut(s) 127
GluI GCNGC 6 cut(s) 138, 247, 565, 608, 641, 873
HaeII RGCGCY 1 cut(s) 129
HaeIII GGCC 4 cut(s) 89, 310, 495, 788
HapII CCGG 3 cut(s) 86, 123, 311
HgaI GACGC 1 cut(s) 204
HhaI GCGC 1 cut(s) 128
Hin1II CATG 4 cut(s) 383, 445, 503, 953
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HindIII AAGCTT 2 cut(s) 327, 402
HinfI GANTC 2 cut(s) 107, 388
HpaII CCGG 3 cut(s) 86, 123, 311
HphI GGTGA 3 cut(s) 116, 179, 245
Hpy166II GTNNAC 1 cut(s) 336
Hpy188I TCNGA 4 cut(s) 118, 205, 354, 919
Hpy188III TCNNGA 6 cut(s) 192, 322, 413, 551, 593, 834
Hpy8I GTNNAC 1 cut(s) 336
HpyAV CCTTC 3 cut(s) 455, 710, 778
HpyCH4III ACNGT 2 cut(s) 694, 805
HpyCH4V TGCA 4 cut(s) 26, 249, 484, 653
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
HpyF3I CTNAG 3 cut(s) 426, 591, 867
Hsp92II CATG 4 cut(s) 383, 445, 503, 953
HspAI GCGC 1 cut(s) 126
KpnI GGTACC 1 cut(s) 766
Kzo9I GATC 4 cut(s) 4, 358, 727, 919
LmnI GCTCC 1 cut(s) 648
Lsp1109I GCAGC 5 cut(s) 233, 551, 619, 652, 884
MaeI CTAG 1 cut(s) 306
MaeIII GTNAC 3 cut(s) 104, 277, 752
MalI GATC 4 cut(s) 6, 360, 729, 921
MboI GATC 4 cut(s) 4, 358, 727, 919
MboII GAAGA 1 cut(s) 180
MfeI CAATTG 1 cut(s) 339
MflI RGATCY 2 cut(s) 4, 727
MluCI AATT 2 cut(s) 339, 897
MlyI GAGTC 1 cut(s) 101
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 9 cut(s) 6, 165, 168, 307, 507, 577, 627, 657, 934
MroXI GAANNNNTTC 1 cut(s) 150
MseI TTAA 3 cut(s) 612, 626, 903
MslI CAYNNNNRTG 2 cut(s) 146, 264
MspCI CTTAAG 1 cut(s) 611
MspI CCGG 3 cut(s) 86, 123, 311
MspR9I CCNGG 1 cut(s) 543
MunI CAATTG 1 cut(s) 339
Mva1269I GAATGC 1 cut(s) 61
MvaI CCWGG 1 cut(s) 543
MvnI CGCG 1 cut(s) 707
MwoI GCNNNNNNNGC 1 cut(s) 134
NdeII GATC 4 cut(s) 4, 358, 727, 919
NlaIII CATG 4 cut(s) 383, 445, 503, 953
NlaIV GGNNCC 4 cut(s) 153, 288, 729, 764
NmuCI GTSAC 1 cut(s) 104
PctI GAATGC 1 cut(s) 61
PdmI GAANNNNTTC 1 cut(s) 150
PfeI GAWTC 1 cut(s) 388
PkrI GCNGC 6 cut(s) 139, 248, 566, 609, 642, 874
PleI GAGTC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 101
Psp6I CCWGG 1 cut(s) 541
PspGI CCWGG 1 cut(s) 541
PspN4I GGNNCC 4 cut(s) 153, 288, 729, 764
PspPI GGNCC 2 cut(s) 494, 553
PsuI RGATCY 2 cut(s) 4, 727
RsaI GTAC 3 cut(s) 30, 335, 764
RsaNI GTAC 3 cut(s) 29, 334, 763
RseI CAYNNNNRTG 2 cut(s) 146, 264
SaqAI TTAA 3 cut(s) 612, 626, 903
SatI GCNGC 6 cut(s) 138, 247, 565, 608, 641, 873
Sau3AI GATC 4 cut(s) 4, 358, 727, 919
Sau96I GGNCC 2 cut(s) 494, 553
ScaI AGTACT 1 cut(s) 30
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 1 cut(s) 543
SgrAI CRCCGGYG 1 cut(s) 122
SinI GGWCC 1 cut(s) 553
SmiMI CAYNNNNRTG 2 cut(s) 146, 264
SmlI CTYRAG 2 cut(s) 611, 631
SmoI CTYRAG 2 cut(s) 611, 631
Sse9I AATT 2 cut(s) 339, 897
SsiI CCGC 1 cut(s) 138
SspMI CTAG 1 cut(s) 306
StyD4I CCNGG 1 cut(s) 541
StyI CCWWGG 1 cut(s) 305
TaaI ACNGT 2 cut(s) 694, 805
TaqI TCGA 3 cut(s) 323, 669, 735
TasI AATT 2 cut(s) 339, 897
TatI WGTACW 2 cut(s) 28, 333
TauI GCSGC 1 cut(s) 140
TfiI GAWTC 1 cut(s) 388
Tru1I TTAA 3 cut(s) 612, 626, 903
Tru9I TTAA 3 cut(s) 612, 626, 903
TscAI CASTG 1 cut(s) 699
TseFI GTSAC 1 cut(s) 104
TseI GCWGC 5 cut(s) 246, 564, 607, 640, 872
Tsp45I GTSAC 1 cut(s) 104
TspDTI ATGAA 3 cut(s) 217, 458, 729
TspRI CASTG 1 cut(s) 699
Vha464I CTTAAG 1 cut(s) 611
VpaK11BI GGWCC 1 cut(s) 553
XcmI CCANNNNNNNNNTGG 1 cut(s) 773
XmaJI CCTAGG 1 cut(s) 305
XmnI GAANNNNTTC 1 cut(s) 150
XspI CTAG 1 cut(s) 306
ZrmI AGTACT 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.