AT3G29180

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005242
Physical Location & Seq
Forward (+)
11148167 .. 11151633
3467 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G29180.2

Sequence Viewer

Length: 1542 bp
ATGGGTGGATGTGTGTCAACCCATTCAAAAGCGATTAGGCCTAGGAGGAAAGGCCGTCGTAGATCTTCCAAACACTTTTCTAAAGTGTCTGATATTGTTCCTCATGCTAATATTAGAAGGCCTAGTGACGTTGGGAGCCGAGTTTCTTTTGCTATCTCGCAAGATGATGCATGGTTTGACTCTGTCAGTGTTCTTGATTCGGATGAGGATGAGGACTTTATCAGCTTACCTGAAGAAAACGTACCATCAACGCCATCAGCGGGTGGTGCGACTGGTAACAATATACCAAATGGTCAAGTAGTTCAGTTTGAATCTTCTTCGTGCTTCGTGGATGGTAAAGGAAAGTACGAAGAATACCATGAGACTTACTTGAAGATAGACGGGAGTAAAGCCGAGAAGTTTGTGTCAAAAGGGATGTACAAGGATCCGAGTGGTCTCTCTGTTCTTACTGGAAACAACAAGAAGAAACTGATGGACCATGCAAGCTTTAAAGGCTTGAAGGATCCAAAACGTAACTCTCAAGAGAAAACCCTGAGAACCAGCCTGAGCCGGTTGATGCCAACAGTCAGTTTTAATGACAAGACTCTAAACTCACCCACATCTCAGAAACGAAAATCCGCTGTTTATCGGCTTTCTTTCAAAAGGAGGTCGTGTGATGGTGAGGAAGTTACTGAGCAACGTAAGTTGTTGTACCGTCCAAAAGCGGGATTCACAATTCCTTCCTCGGGCAGAGAAAAGCAGTCTAGCGGAAGTTGGTCTGAGATACCGCCTTCAACTTTTAAACTCCGTGGAGAAACCTATTTCAAAGACAAAAAGAAATCCCCAGCTCCAAATCAGTGTCCATACACTCCTATAGGTGTTGACTTGTTTGTCTGTCCAAGGAAGATTGATCATATCGCCCAACACATCGAGCTTCCAAACATCAAAGCCGAGGCAAAGCTCCCTGCTCTTCTTGTTGTCAACATTCAGTTACCAACCTATCCTGCTGCAATGTTCCTTGGGGACAGTGATGGAGAAGGCATGAGCATTGTACTTTATTTTAAATTACGAGACAATCACGAAAAAGAAACCTCTCAGCAATATCAGGAGAGCATCAAGAAACTTGTCAATGATGAGATGGAGAAGGTAAAAGGGTTTGCTAAAGACAGCAACGTTGCTTTCCGTGAAAGGCTTAAGATAGTAGCTGGACTTGTTAACCCCGAAGATCTAGCTCTAAGCTCGACTGAAAAGAAGCTTGTACAGGCTTACAATGAAAAACCTGTCCTCTCTCGTCCTCAACACAACTTCTTTAAGGGTCCAAACTACTTCGAGATTGATCTGGATGTACATCGATTCAGCTACATATCTCGAAAAGGACTTGAAGCATTCAGAGATCGACTAAAAAACGGAACTCTTGATCTCGGCTTAACCATCCAGGCTCAAAAACCGGAAGAGCTGCCGGAACAAGTCCTATGCTGTCTGAGGCTGAGCAAGATTGACTTCGTAGACCACGGTCAGATCCCAATGCTTTTGATCCCGGAGGATGGGGAAACTCAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

513

Amino Acids

57.66

Weight (kDa)

9.1

Isoelectric Point (pI)

48.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 252 - 493 1.5e-64 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 869
AccI GTMKAC 1 cut(s) 1485
AciI CCGC 5 cut(s) 260, 618, 704, 747, 767
AclI AACGTT 1 cut(s) 1152
AclWI GGATC 6 cut(s) 419, 432, 497, 510, 1492, 1507
AcuI CTGAAG 1 cut(s) 252
AfaI GTAC 7 cut(s) 243, 347, 419, 692, 1032, 1239, 1326
AfiI CCNNNNNNNGG 4 cut(s) 260, 704, 725, 1523
AflII CTTAAG 1 cut(s) 1172
AgsI TTSAA 8 cut(s) 27, 311, 373, 499, 640, 774, 805, 1361
AjnI CCWGG 1 cut(s) 1413
Alw26I GTCTC 3 cut(s) 356, 440, 1044
AlwI GGATC 6 cut(s) 419, 432, 497, 510, 1492, 1507
Ama87I CYCGRG 1 cut(s) 724
AoxI GGCC 3 cut(s) 38, 52, 119
ApeKI GCWGC 2 cut(s) 986, 1435
AspA2I CCTAGG 1 cut(s) 41
AspS9I GGNCC 2 cut(s) 475, 1295
AsuC2I CCSGG 1 cut(s) 1517
AsuHPI GGTGA 2 cut(s) 585, 671
AvaI CYCGRG 1 cut(s) 724
AvaII GGWCC 2 cut(s) 475, 1295
AvrII CCTAGG 1 cut(s) 41
BamHI GGATCC 2 cut(s) 424, 502
BarI GAAGNNNNNNTAC 2 cut(s) 225, 257
BbvI GCAGC 2 cut(s) 973, 1422
BccI CCATC 9 cut(s) 253, 262, 326, 466, 650, 1004, 1111, 1418, 1517
BceAI ACGGC 1 cut(s) 39
BciT130I CCWGG 1 cut(s) 1415
BclI TGATCA 1 cut(s) 889
BcnI CCSGG 1 cut(s) 1517
BcoDI GTCTC 3 cut(s) 356, 440, 1044
BfaI CTAG 4 cut(s) 42, 123, 744, 1208
BfmI CTRYAG 1 cut(s) 852
BfrI CTTAAG 1 cut(s) 1172
BglII AGATCT 2 cut(s) 62, 1204
BisI GCNGC 2 cut(s) 987, 1436
BlnI CCTAGG 1 cut(s) 41
BlpI GCTNAGC 1 cut(s) 1466
BlsI GCNGC 2 cut(s) 988, 1437
Bme1390I CCNGG 2 cut(s) 1415, 1517
Bme18I GGWCC 2 cut(s) 475, 1295
BmeT110I CYCGRG 1 cut(s) 724
BmgT120I GGNCC 2 cut(s) 475, 1295
BmiI GGNNCC 4 cut(s) 137, 426, 504, 1296
BmrFI CCNGG 2 cut(s) 1415, 1517
BmsI GCATC 3 cut(s) 157, 546, 1101
BoxI GACNNNNGTC 1 cut(s) 1491
Bpu10I CCTNAGC 1 cut(s) 545
Bpu1102I GCTNAGC 1 cut(s) 1466
BpuEI CTTGAG 2 cut(s) 504, 1518
BpuMI CCSGG 1 cut(s) 1517
Bsa29I ATCGAT 1 cut(s) 1330
BsaBI GATNNNNATC 1 cut(s) 1326
BsaI GGTCTC 1 cut(s) 440
BsaJI CCNNGG 7 cut(s) 41, 723, 787, 878, 930, 997, 1489
BsaWI WCCGGW 1 cut(s) 1426
BsaXI ACNNNNNCTCC 2 cut(s) 637, 667
Bsc4I CCNNNNNNNGG 4 cut(s) 260, 704, 725, 1523
Bse118I RCCGGY 1 cut(s) 549
Bse1I ACTGG 2 cut(s) 277, 454
Bse3DI GCAATG 1 cut(s) 996
Bse8I GATNNNNATC 1 cut(s) 1326
BseBI CCWGG 1 cut(s) 1415
BseCI ATCGAT 1 cut(s) 1330
BseDI CCNNGG 7 cut(s) 41, 723, 787, 878, 930, 997, 1489
BseGI GGATG 8 cut(s) 14, 208, 214, 337, 420, 1327, 1410, 1528
BseJI GATNNNNATC 1 cut(s) 1326
BseLI CCNNNNNNNGG 4 cut(s) 260, 704, 725, 1523
BseMI GCAATG 1 cut(s) 996
BseMII CTCAG 8 cut(s) 524, 536, 617, 663, 750, 1088, 1451, 1457
BseNI ACTGG 2 cut(s) 277, 454
BseXI GCAGC 2 cut(s) 973, 1422
BseYI CCCAGC 1 cut(s) 823
BshFI GGCC 3 cut(s) 40, 54, 121
BshVI ATCGAT 1 cut(s) 1330
BsiHKCI CYCGRG 1 cut(s) 724
BsiSI CCGG 4 cut(s) 550, 1427, 1439, 1517
BslFI GGGAC 1 cut(s) 1016
BslI CCNNNNNNNGG 4 cut(s) 260, 704, 725, 1523
BsmAI GTCTC 3 cut(s) 356, 440, 1044
BsmFI GGGAC 1 cut(s) 1016
BsmI GAATGC 1 cut(s) 1364
BsnI GGCC 3 cut(s) 40, 54, 121
Bso31I GGTCTC 1 cut(s) 440
BsoBI CYCGRG 1 cut(s) 724
Bsp1407I TGTACA 3 cut(s) 417, 1237, 1324
Bsp1720I GCTNAGC 1 cut(s) 1466
BspACI CCGC 5 cut(s) 260, 618, 704, 747, 767
BspANI GGCC 3 cut(s) 40, 54, 121
BspCNI CTCAG 8 cut(s) 525, 537, 616, 664, 751, 1087, 1452, 1458
BspDI ATCGAT 1 cut(s) 1330
BspLI GGNNCC 4 cut(s) 137, 426, 504, 1296
BspPI GGATC 6 cut(s) 419, 432, 497, 510, 1492, 1507
BspQI GCTCTTC 2 cut(s) 954, 1425
BspTI CTTAAG 1 cut(s) 1172
BspTNI GGTCTC 1 cut(s) 440
BsrDI GCAATG 1 cut(s) 996
BsrFI RCCGGY 1 cut(s) 549
BsrGI TGTACA 3 cut(s) 417, 1237, 1324
BsrI ACTGG 2 cut(s) 277, 454
BssAI RCCGGY 1 cut(s) 549
BssECI CCNNGG 7 cut(s) 41, 723, 787, 878, 930, 997, 1489
BssT1I CCWWGG 3 cut(s) 41, 878, 997
Bst2UI CCWGG 1 cut(s) 1415
Bst4CI ACNGT 4 cut(s) 565, 695, 1007, 1493
Bst6I CTCTTC 2 cut(s) 954, 1425
BstAFI CTTAAG 1 cut(s) 1172
BstAUI TGTACA 3 cut(s) 417, 1237, 1324
BstC8I GCNNGC 1 cut(s) 484
BstDEI CTNAG 9 cut(s) 533, 545, 603, 672, 759, 1074, 1214, 1460, 1466
BstDSI CCRYGG 2 cut(s) 787, 1489
BstF5I GGATG 8 cut(s) 14, 208, 214, 337, 420, 1327, 1410, 1528
BstMAI GTCTC 3 cut(s) 356, 440, 1044
BstMWI GCNNNNNNNGC 2 cut(s) 266, 492
BstNI CCWGG 1 cut(s) 1415
BstPAI GACNNNNGTC 1 cut(s) 1491
BstSCI CCNGG 2 cut(s) 1413, 1515
BstSFI CTRYAG 1 cut(s) 852
BstV1I GCAGC 2 cut(s) 973, 1422
BstX2I RGATCY 5 cut(s) 62, 424, 502, 1204, 1497
BstYI RGATCY 5 cut(s) 62, 424, 502, 1204, 1497
Bsu15I ATCGAT 1 cut(s) 1330
BsuRI GGCC 3 cut(s) 40, 54, 121
BsuTUI ATCGAT 1 cut(s) 1330
BtgI CCRYGG 2 cut(s) 787, 1489
BtsCI GGATG 8 cut(s) 14, 208, 214, 337, 420, 1327, 1410, 1528
BtsIMutI CAGTG 3 cut(s) 193, 842, 1012
Cac8I GCNNGC 1 cut(s) 484
Cfr10I RCCGGY 1 cut(s) 549
Cfr13I GGNCC 2 cut(s) 475, 1295
ClaI ATCGAT 1 cut(s) 1330
Csp6I GTAC 7 cut(s) 242, 346, 418, 691, 1031, 1238, 1325
CviAII CATG 5 cut(s) 104, 171, 359, 479, 1021
CviQI GTAC 7 cut(s) 242, 346, 418, 691, 1031, 1238, 1325
DdeI CTNAG 9 cut(s) 533, 545, 603, 672, 759, 1074, 1214, 1460, 1466
DraI TTTAAA 3 cut(s) 490, 781, 1042
DrdI GACNNNNNNGTC 1 cut(s) 869
DseDI GACNNNNNNGTC 1 cut(s) 869
Eam1104I CTCTTC 2 cut(s) 954, 1425
EarI CTCTTC 2 cut(s) 954, 1425
Eco130I CCWWGG 3 cut(s) 41, 878, 997
Eco147I AGGCCT 2 cut(s) 40, 121
Eco31I GGTCTC 1 cut(s) 440
Eco47I GGWCC 2 cut(s) 475, 1295
Eco57I CTGAAG 1 cut(s) 252
Eco88I CYCGRG 1 cut(s) 724
EcoRII CCWGG 1 cut(s) 1413
EcoT14I CCWWGG 3 cut(s) 41, 878, 997
EcoT22I ATGCAT 1 cut(s) 172
ErhI CCWWGG 3 cut(s) 41, 878, 997
FaeI CATG 5 cut(s) 107, 174, 362, 482, 1024
FalI AAGNNNNNCTT 2 cut(s) 1463, 1495
FaqI GGGAC 1 cut(s) 1016
FatI CATG 5 cut(s) 103, 170, 358, 478, 1020
FauI CCCGC 2 cut(s) 253, 697
FbaI TGATCA 1 cut(s) 889
FblI GTMKAC 1 cut(s) 1485
Fnu4HI GCNGC 2 cut(s) 987, 1436
FokI GGATG 8 cut(s) 21, 215, 221, 344, 427, 1334, 1397, 1535
Fsp4HI GCNGC 2 cut(s) 987, 1436
FspBI CTAG 4 cut(s) 42, 123, 744, 1208
GluI GCNGC 2 cut(s) 987, 1436
GsaI CCCAGC 1 cut(s) 827
HaeIII GGCC 3 cut(s) 40, 54, 121
HapII CCGG 4 cut(s) 550, 1427, 1439, 1517
Hin1II CATG 5 cut(s) 107, 174, 362, 482, 1024
HincII GTYRAC 4 cut(s) 18, 862, 961, 1195
HindII GTYRAC 4 cut(s) 18, 862, 961, 1195
HindIII AAGCTT 2 cut(s) 484, 1232
HinfI GANTC 6 cut(s) 179, 197, 311, 583, 708, 1332
HpaI GTTAAC 1 cut(s) 1195
HpaII CCGG 4 cut(s) 550, 1427, 1439, 1517
HphI GGTGA 2 cut(s) 585, 671
Hpy166II GTNNAC 5 cut(s) 18, 862, 961, 1195, 1486
Hpy188I TCNGA 8 cut(s) 91, 202, 429, 606, 760, 1370, 1461, 1497
Hpy188III TCNNGA 9 cut(s) 194, 521, 1058, 1085, 1096, 1309, 1319, 1347, 1394
Hpy8I GTNNAC 5 cut(s) 18, 862, 961, 1195, 1486
Hpy99I CGWCG 1 cut(s) 60
HpyAV CCTTC 6 cut(s) 111, 493, 729, 780, 1010, 1117
HpyCH4III ACNGT 4 cut(s) 565, 695, 1007, 1493
HpyCH4IV ACGT 5 cut(s) 129, 240, 511, 679, 1152
HpyCH4V TGCA 3 cut(s) 170, 482, 989
HpyF10VI GCNNNNNNNGC 2 cut(s) 266, 492
HpyF3I CTNAG 9 cut(s) 533, 545, 603, 672, 759, 1074, 1214, 1460, 1466
HpySE526I ACGT 5 cut(s) 129, 240, 511, 679, 1152
Hsp92II CATG 5 cut(s) 107, 174, 362, 482, 1024
Ksp22I TGATCA 1 cut(s) 889
KspAI GTTAAC 1 cut(s) 1195
LguI GCTCTTC 2 cut(s) 954, 1425
LmnI GCTCC 3 cut(s) 135, 832, 945
Lsp1109I GCAGC 2 cut(s) 973, 1422
LweI GCATC 3 cut(s) 157, 546, 1101
MaeI CTAG 4 cut(s) 42, 123, 744, 1208
MaeII ACGT 5 cut(s) 129, 240, 511, 679, 1152
MaeIII GTNAC 5 cut(s) 125, 275, 512, 667, 969
MflI RGATCY 5 cut(s) 62, 424, 502, 1204, 1497
MluCI AATT 2 cut(s) 714, 1043
MlyI GAGTC 2 cut(s) 173, 577
Mph1103I ATGCAT 1 cut(s) 172
MseI TTAA 8 cut(s) 489, 573, 780, 1041, 1173, 1194, 1290, 1406
MspA1I CMGCKG 2 cut(s) 260, 620
MspCI CTTAAG 1 cut(s) 1172
MspI CCGG 4 cut(s) 550, 1427, 1439, 1517
MspR9I CCNGG 2 cut(s) 1415, 1517
Mva1269I GAATGC 1 cut(s) 1364
MvaI CCWGG 1 cut(s) 1415
MwoI GCNNNNNNNGC 2 cut(s) 266, 492
NciI CCSGG 1 cut(s) 1517
NlaIII CATG 5 cut(s) 107, 174, 362, 482, 1024
NlaIV GGNNCC 4 cut(s) 137, 426, 504, 1296
NmeAIII GCCGAG 4 cut(s) 164, 418, 955, 1380
NmuCI GTSAC 1 cut(s) 125
NsiI ATGCAT 1 cut(s) 172
PceI AGGCCT 2 cut(s) 40, 121
PciSI GCTCTTC 2 cut(s) 954, 1425
PctI GAATGC 1 cut(s) 1364
PfeI GAWTC 4 cut(s) 197, 311, 708, 1332
PflFI GACNNNGTC 1 cut(s) 182
PfoI TCCNGGA 1 cut(s) 1515
PkrI GCNGC 2 cut(s) 988, 1437
PleI GAGTC 2 cut(s) 173, 577
PpsI GAGTC 2 cut(s) 173, 577
PshAI GACNNNNGTC 1 cut(s) 1491
Psp1406I AACGTT 1 cut(s) 1152
Psp6I CCWGG 1 cut(s) 1413
PspFI CCCAGC 1 cut(s) 823
PspGI CCWGG 1 cut(s) 1413
PspN4I GGNNCC 4 cut(s) 137, 426, 504, 1296
PspPI GGNCC 2 cut(s) 475, 1295
PsuI RGATCY 5 cut(s) 62, 424, 502, 1204, 1497
PsyI GACNNNGTC 1 cut(s) 182
RsaI GTAC 7 cut(s) 243, 347, 419, 692, 1032, 1239, 1326
RsaNI GTAC 7 cut(s) 242, 346, 418, 691, 1031, 1238, 1325
SapI GCTCTTC 2 cut(s) 954, 1425
SaqAI TTAA 8 cut(s) 489, 573, 780, 1041, 1173, 1194, 1290, 1406
SatI GCNGC 2 cut(s) 987, 1436
Sau96I GGNCC 2 cut(s) 475, 1295
SchI GAGTC 2 cut(s) 173, 577
ScrFI CCNGG 2 cut(s) 1415, 1517
SfaNI GCATC 3 cut(s) 157, 546, 1101
SfcI CTRYAG 1 cut(s) 852
SinI GGWCC 2 cut(s) 475, 1295
SmlI CTYRAG 3 cut(s) 519, 1172, 1533
SmoI CTYRAG 3 cut(s) 519, 1172, 1533
Sse9I AATT 2 cut(s) 714, 1043
SseBI AGGCCT 2 cut(s) 40, 121
SsiI CCGC 5 cut(s) 260, 618, 704, 747, 767
SspI AATATT 1 cut(s) 112
SspMI CTAG 4 cut(s) 42, 123, 744, 1208
StuI AGGCCT 2 cut(s) 40, 121
StyD4I CCNGG 2 cut(s) 1413, 1515
StyI CCWWGG 3 cut(s) 41, 878, 997
TaaI ACNGT 4 cut(s) 565, 695, 1007, 1493
TaiI ACGT 5 cut(s) 132, 243, 514, 682, 1155
TaqI TCGA 6 cut(s) 909, 1220, 1308, 1330, 1348, 1375
TasI AATT 2 cut(s) 714, 1043
TatI WGTACW 4 cut(s) 417, 1030, 1237, 1324
TfiI GAWTC 4 cut(s) 197, 311, 708, 1332
Tru1I TTAA 8 cut(s) 489, 573, 780, 1041, 1173, 1194, 1290, 1406
Tru9I TTAA 8 cut(s) 489, 573, 780, 1041, 1173, 1194, 1290, 1406
TscAI CASTG 3 cut(s) 193, 842, 1012
TseFI GTSAC 1 cut(s) 125
TseI GCWGC 2 cut(s) 986, 1435
Tsp45I GTSAC 1 cut(s) 125
TspDTI ATGAA 1 cut(s) 1266
TspGWI ACGGA 3 cut(s) 776, 1151, 1401
TspRI CASTG 3 cut(s) 193, 842, 1012
Tth111I GACNNNGTC 1 cut(s) 182
Vha464I CTTAAG 1 cut(s) 1172
VpaK11BI GGWCC 2 cut(s) 475, 1295
XmaJI CCTAGG 1 cut(s) 41
XmiI GTMKAC 1 cut(s) 1485
XspI CTAG 4 cut(s) 42, 123, 744, 1208
Zsp2I ATGCAT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.