MD17G1053900.v1.1

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005242
Physical Location & Seq
Forward (+)
4185496 .. 4190992
5497 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1053900.v1.1.491

Sequence Viewer

Length: 1677 bp
ATGGGTGGTTGCGTGTCAACCCCGTCCAGGACAATTAAATCGCGGAAGAAACACCATCAGAGGGTCAGCAAACGCCATGGAAAGATAACCTGCTCTGCTTCCGATGGTACCAAGAAAAGAAATAGCGGTGCCAAGAAGAGAAATAGCGATGCAGGAGCTCGCGTAACTGATTATTCTGTGAGTGAGTTTGTTCATATGGACTTTGAGAATGGTGCAACAACTACTCGCAGAAGATCTAAGGTTTCTAGTTCGACGTTCCAACTCACCCAAATGCAGTGGCACCACCAATATGATTCAAATGTAATATCCCAAGAGGAAGCTTGGTTCGACTCGGTGAGTATTCTGGAGTCCGACTCGGATGATGAATTTATCAGTATACACGGAGATGGTTTTCCATTAGGAGGCAACCCAATTGGGAATATCTCAGGTGGCCAAGTCGTTCAGTACGAAAGATCTTCACACTTTGTGGATAACGGGTGTAAGTATGAAGAATACCAAAGCTATACGAAAATAGATGGAGGTGTATCAGATAAGTTCATGGGGAAGGCTGACGAACCGCGTAGCAAGAGGAAGAATATTTTGGACCATTCTTATGGAAGCTTTAAAGGACTTAGAGAGGATCGACGTGATTCTAATGAAAAAATTCAAGACAGTATGCTTAAATCTGGCTTACCTCGATTGGTACCTTCTGTAAGTTTCAACGATAAGATTTTAAGTGCACAGAGTTTGGGTCCACAGGCGCAAAGAAAGCCATCGGCAGTTTTCAGGCTTTCTTTTAAGAGGAGATCGTGTGATGCAGACGAAACCATTGAACAATGTCAATCAAGAAGGTTTCTGTATCGTCCTAGACCAGGATATATCATTCCATGTTGTAGAGTGGAAAAGCCGACTCCAGGATCTTGGTCTGAGATTCCACCCTCAACTTTTAAACTCCGTGGGGAGAACTATTTCAAGGATAAACGCAAGTCACCTGCTCCAAATTACAGTCCATATACTCCAATAGGTGTTGATCTATTTGTATGCCCCAAGAAGATACATCACATTGCCCAGCATCTTGAGCTTCCCAAAGTAAAAGCCGATGGAAAAGTGCCTCCGTTCCTCATCGTAAATATACAGTTGCCTACTTATCCCGCTGCAATGTTCCTTGGTGATAGTGATGGAGAAGGAATTAGTCTCGTAATGTATTTCAAAGTTTCTGAGAATTTTGAGAAAGACATCTCTCCTCAATTCCAGGACAGCATCAAGAAATTGGTTGACGATGAAACGGAAAAGGTTAAAGGATTTGCGAAGGACTCCATTGTACCCTTTAGAGAAAGGCTAAAGATCATGGCAGGGTTGGTTAATCCTGAGGATCTCGGTCTGAGTTCTGCTGAAAAGAAGCTTGTAAATGCATATAACGAAAAACCAGTTCTTTCCCGTCCTCAGCACAATTTTTATAAGGGTCCTAATTACTTTGAGATCGATCTGGACATTCATCGCTTCAGCTACATATCGAGGAAGGGTCTCGAATCTTTTAGAGAACGTCTAAAAAACGGAATTCTGGACATGGGTTTAACCATCCAGGCACAAAAACAGGAAGAACTGCCGGAGGAACTGCTCTGTTGTATGAGGCTGAACAAGATTGATTTTGTTGATCACGGCCAAATACCGAGTCTAGTGACCATTGACGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

559

Amino Acids

63.23

Weight (kDa)

8.88

Isoelectric Point (pI)

48.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 301 - 542 1.1e-64 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1437
AarI CACCTGC 1 cut(s) 979
Acc36I ACCTGC 2 cut(s) 98, 979
Acc65I GGTACC 2 cut(s) 107, 682
AccB1I GGYRCC 4 cut(s) 107, 128, 279, 682
AccI GTMKAC 1 cut(s) 376
AccII CGCG 3 cut(s) 43, 162, 559
AciI CCGC 4 cut(s) 43, 126, 557, 1131
AclWI GGATC 3 cut(s) 627, 904, 1359
AcoI YGGCCR 2 cut(s) 430, 1639
AcsI RAATTY 4 cut(s) 365, 642, 1201, 1536
AcuI CTGAAG 1 cut(s) 1465
AdeI CACNNNGTG 1 cut(s) 466
AfaI GTAC 4 cut(s) 109, 446, 684, 1302
AfiI CCNNNNNNNGG 5 cut(s) 27, 61, 401, 851, 893
AgsI TTSAA 6 cut(s) 297, 647, 700, 812, 952, 1189
AjiI CACGTC 1 cut(s) 626
AjnI CCWGG 5 cut(s) 26, 850, 892, 1230, 1560
AjuI GAANNNNNNNTTGG 2 cut(s) 563, 595
AloI GAACNNNNNNTCC 2 cut(s) 308, 340
AluBI AGCT 7 cut(s) 158, 320, 501, 600, 1060, 1381, 1485
AluI AGCT 7 cut(s) 158, 320, 501, 600, 1060, 1381, 1485
Alw21I GWGCWC 2 cut(s) 160, 721
Alw26I GTCTC 2 cut(s) 1178, 1508
Alw44I GTGCAC 1 cut(s) 717
AlwI GGATC 3 cut(s) 627, 904, 1359
AoxI GGCC 2 cut(s) 430, 1639
ApaLI GTGCAC 1 cut(s) 717
ApeKI GCWGC 1 cut(s) 1133
ApoI RAATTY 4 cut(s) 365, 642, 1201, 1536
ArsI GACNNNNNNTTYG 2 cut(s) 1636, 1668
Asp700I GAANNNNTTC 2 cut(s) 642, 947
Asp718I GGTACC 2 cut(s) 107, 682
AspLEI GCGC 1 cut(s) 742
AspS9I GGNCC 3 cut(s) 583, 731, 1442
AsuHPI GGTGA 4 cut(s) 256, 346, 960, 1160
AvaII GGWCC 3 cut(s) 583, 731, 1442
AxyI CCTNAGG 1 cut(s) 1347
BaeGI GKGCMC 1 cut(s) 721
BalI TGGCCA 1 cut(s) 432
BanI GGYRCC 4 cut(s) 107, 128, 279, 682
BanII GRGCYC 1 cut(s) 160
Bbv12I GWGCWC 2 cut(s) 160, 721
BbvCI CCTCAGC 1 cut(s) 1422
BbvI GCAGC 1 cut(s) 1120
BccI CCATC 8 cut(s) 63, 98, 380, 509, 760, 1073, 1151, 1565
BceAI ACGGC 1 cut(s) 1654
BciT130I CCWGG 5 cut(s) 28, 852, 894, 1232, 1562
BclI TGATCA 1 cut(s) 1633
BcoDI GTCTC 2 cut(s) 1178, 1508
BfaI CTAG 3 cut(s) 246, 846, 1655
BfuAI ACCTGC 2 cut(s) 98, 979
BglII AGATCT 2 cut(s) 233, 452
BisI GCNGC 1 cut(s) 1134
BlsI GCNGC 1 cut(s) 1135
Bme1390I CCNGG 5 cut(s) 28, 852, 894, 1232, 1562
Bme18I GGWCC 3 cut(s) 583, 731, 1442
BmgBI CACGTC 1 cut(s) 626
BmgT120I GGNCC 3 cut(s) 583, 731, 1442
BmiI GGNNCC 6 cut(s) 109, 130, 281, 684, 732, 1443
BmrFI CCNGG 5 cut(s) 28, 852, 894, 1232, 1562
BmsI GCATC 4 cut(s) 139, 784, 1060, 1248
BplI GAGNNNNNCTC 2 cut(s) 338, 370
BpmI CTGGAG 2 cut(s) 365, 876
Bpu10I CCTNAGC 1 cut(s) 1422
BpuEI CTTGAG 1 cut(s) 1076
Bsa29I ATCGAT 1 cut(s) 1461
BsaI GGTCTC 1 cut(s) 1508
BsaJI CCNNGG 3 cut(s) 76, 934, 1144
BsaXI ACNNNNNCTCC 4 cut(s) 147, 177, 775, 805
Bsc4I CCNNNNNNNGG 5 cut(s) 27, 61, 401, 851, 893
Bse1I ACTGG 1 cut(s) 1406
Bse21I CCTNAGG 1 cut(s) 1347
Bse3DI GCAATG 2 cut(s) 1041, 1143
BseBI CCWGG 5 cut(s) 28, 852, 894, 1232, 1562
BseCI ATCGAT 1 cut(s) 1461
BseDI CCNNGG 3 cut(s) 76, 934, 1144
BseGI GGATG 2 cut(s) 364, 1557
BseLI CCNNNNNNNGG 5 cut(s) 27, 61, 401, 851, 893
BseMI GCAATG 2 cut(s) 1041, 1143
BseMII CTCAG 6 cut(s) 438, 897, 1188, 1338, 1352, 1436
BseNI ACTGG 1 cut(s) 1406
BseRI GAGGAG 2 cut(s) 796, 1212
BseSI GKGCMC 1 cut(s) 721
BseXI GCAGC 1 cut(s) 1120
BseYI CCCAGC 1 cut(s) 1047
Bsh1236I CGCG 3 cut(s) 43, 162, 559
BshFI GGCC 2 cut(s) 432, 1641
BshNI GGYRCC 4 cut(s) 107, 128, 279, 682
BshVI ATCGAT 1 cut(s) 1461
BsiHKAI GWGCWC 2 cut(s) 160, 721
BsiSI CCGG 1 cut(s) 1586
BslI CCNNNNNNNGG 5 cut(s) 27, 61, 401, 851, 893
BsmAI GTCTC 2 cut(s) 1178, 1508
BsnI GGCC 2 cut(s) 432, 1641
Bso31I GGTCTC 1 cut(s) 1508
Bsp1286I GDGCHC 2 cut(s) 160, 721
Bsp19I CCATGG 1 cut(s) 76
BspACI CCGC 4 cut(s) 43, 126, 557, 1131
BspANI GGCC 2 cut(s) 432, 1641
BspCNI CTCAG 6 cut(s) 437, 898, 1189, 1339, 1353, 1435
BspDI ATCGAT 1 cut(s) 1461
BspFNI CGCG 3 cut(s) 43, 162, 559
BspLI GGNNCC 6 cut(s) 109, 130, 281, 684, 732, 1443
BspMI ACCTGC 2 cut(s) 98, 979
BspPI GGATC 3 cut(s) 627, 904, 1359
BspT107I GGYRCC 4 cut(s) 107, 128, 279, 682
BspTNI GGTCTC 1 cut(s) 1508
BsrDI GCAATG 2 cut(s) 1041, 1143
BsrI ACTGG 1 cut(s) 1406
BssECI CCNNGG 3 cut(s) 76, 934, 1144
BssNAI GTATAC 1 cut(s) 377
BssT1I CCWWGG 2 cut(s) 76, 1144
Bst1107I GTATAC 1 cut(s) 377
Bst2UI CCWGG 5 cut(s) 28, 852, 894, 1232, 1562
Bst4CI ACNGT 3 cut(s) 653, 986, 1116
Bst6I CTCTTC 1 cut(s) 131
BstC8I GCNNGC 1 cut(s) 160
BstDEI CTNAG 8 cut(s) 237, 424, 611, 906, 1197, 1347, 1361, 1422
BstDSI CCRYGG 2 cut(s) 76, 934
BstENI CCTNNNNNAGG 1 cut(s) 849
BstF5I GGATG 2 cut(s) 364, 1557
BstFNI CGCG 3 cut(s) 43, 162, 559
BstHHI GCGC 1 cut(s) 742
BstMAI GTCTC 2 cut(s) 1178, 1508
BstMWI GCNNNNNNNGC 2 cut(s) 748, 1057
BstNI CCWGG 5 cut(s) 28, 852, 894, 1232, 1562
BstSCI CCNGG 5 cut(s) 26, 850, 892, 1230, 1560
BstSLI GKGCMC 1 cut(s) 721
BstUI CGCG 3 cut(s) 43, 162, 559
BstV1I GCAGC 1 cut(s) 1120
BstX2I RGATCY 4 cut(s) 233, 452, 896, 1351
BstXI CCANNNNNNTGG 2 cut(s) 593, 900
BstYI RGATCY 4 cut(s) 233, 452, 896, 1351
BstZ17I GTATAC 1 cut(s) 377
Bsu15I ATCGAT 1 cut(s) 1461
Bsu36I CCTNAGG 1 cut(s) 1347
BsuRI GGCC 2 cut(s) 432, 1641
BsuTUI ATCGAT 1 cut(s) 1461
BtgI CCRYGG 2 cut(s) 76, 934
BtgZI GCGATG 2 cut(s) 162, 1460
BtrI CACGTC 1 cut(s) 626
BtsCI GGATG 2 cut(s) 364, 1557
BtsI GCAGTG 1 cut(s) 281
BtsIMutI CAGTG 1 cut(s) 281
BveI ACCTGC 2 cut(s) 98, 979
Cac8I GCNNGC 1 cut(s) 160
CfoI GCGC 1 cut(s) 742
Cfr13I GGNCC 3 cut(s) 583, 731, 1442
ClaI ATCGAT 1 cut(s) 1461
Csp6I GTAC 4 cut(s) 108, 445, 683, 1301
CspCI CAANNNNNGTGG 2 cut(s) 257, 292
CviAII CATG 5 cut(s) 77, 538, 867, 1327, 1546
CviQI GTAC 4 cut(s) 108, 445, 683, 1301
DdeI CTNAG 8 cut(s) 237, 424, 611, 906, 1197, 1347, 1361, 1422
DraI TTTAAA 2 cut(s) 604, 928
DraIII CACNNNGTG 1 cut(s) 466
EaeI YGGCCR 2 cut(s) 430, 1639
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
Ecl136II GAGCTC 1 cut(s) 158
Eco130I CCWWGG 2 cut(s) 76, 1144
Eco24I GRGCYC 1 cut(s) 160
Eco31I GGTCTC 1 cut(s) 1508
Eco47I GGWCC 3 cut(s) 583, 731, 1442
Eco53kI GAGCTC 1 cut(s) 158
Eco57I CTGAAG 1 cut(s) 1465
Eco81I CCTNAGG 1 cut(s) 1347
EcoICRI GAGCTC 1 cut(s) 158
EcoNI CCTNNNNNAGG 1 cut(s) 849
EcoO109I RGGNCCY 1 cut(s) 1442
EcoRI GAATTC 1 cut(s) 1536
EcoRII CCWGG 5 cut(s) 26, 850, 892, 1230, 1560
EcoT14I CCWWGG 2 cut(s) 76, 1144
EcoT22I ATGCAT 1 cut(s) 1393
EcoT38I GRGCYC 1 cut(s) 160
ErhI CCWWGG 2 cut(s) 76, 1144
FaeI CATG 5 cut(s) 80, 541, 870, 1330, 1549
FatI CATG 5 cut(s) 76, 537, 866, 1326, 1545
FauI CCCGC 1 cut(s) 1138
FauNDI CATATG 1 cut(s) 195
FbaI TGATCA 1 cut(s) 1633
FblI GTMKAC 1 cut(s) 376
Fnu4HI GCNGC 1 cut(s) 1134
FokI GGATG 2 cut(s) 371, 1544
FriOI GRGCYC 1 cut(s) 160
Fsp4HI GCNGC 1 cut(s) 1134
FspBI CTAG 3 cut(s) 246, 846, 1655
GlaI GCGC 1 cut(s) 741
GluI GCNGC 1 cut(s) 1134
GsaI CCCAGC 1 cut(s) 1051
GsuI CTGGAG 2 cut(s) 365, 876
HaeIII GGCC 2 cut(s) 432, 1641
HapII CCGG 1 cut(s) 1586
HhaI GCGC 1 cut(s) 742
Hin1II CATG 5 cut(s) 80, 541, 870, 1330, 1549
Hin6I GCGC 1 cut(s) 740
HinP1I GCGC 1 cut(s) 740
HincII GTYRAC 2 cut(s) 18, 1255
HindII GTYRAC 2 cut(s) 18, 1255
HindIII AAGCTT 3 cut(s) 318, 598, 1379
HpaII CCGG 1 cut(s) 1586
HphI GGTGA 4 cut(s) 256, 346, 960, 1160
Hpy166II GTNNAC 5 cut(s) 18, 377, 719, 734, 1255
Hpy188I TCNGA 8 cut(s) 60, 103, 352, 358, 529, 907, 1198, 1362
Hpy188III TCNNGA 9 cut(s) 344, 647, 825, 1055, 1243, 1346, 1466, 1505, 1541
Hpy8I GTNNAC 5 cut(s) 18, 377, 719, 734, 1255
Hpy99I CGWCG 2 cut(s) 256, 627
HpyAV CCTTC 6 cut(s) 538, 696, 822, 1157, 1282, 1492
HpyCH4III ACNGT 3 cut(s) 653, 986, 1116
HpyCH4IV ACGT 3 cut(s) 254, 625, 1522
HpyCH4V TGCA 7 cut(s) 152, 215, 274, 719, 797, 1136, 1391
HpyF10VI GCNNNNNNNGC 2 cut(s) 748, 1057
HpyF3I CTNAG 8 cut(s) 237, 424, 611, 906, 1197, 1347, 1361, 1422
HpySE526I ACGT 3 cut(s) 254, 625, 1522
Hsp92II CATG 5 cut(s) 80, 541, 870, 1330, 1549
HspAI GCGC 1 cut(s) 740
KpnI GGTACC 2 cut(s) 111, 686
Ksp22I TGATCA 1 cut(s) 1633
LmnI GCTCC 2 cut(s) 155, 979
Lsp1109I GCAGC 1 cut(s) 1120
LweI GCATC 4 cut(s) 139, 784, 1060, 1248
MaeI CTAG 3 cut(s) 246, 846, 1655
MaeII ACGT 3 cut(s) 254, 625, 1522
MaeIII GTNAC 3 cut(s) 163, 966, 1657
MboII GAAGA 8 cut(s) 58, 148, 243, 447, 500, 583, 1042, 1589
MfeI CAATTG 1 cut(s) 411
MflI RGATCY 4 cut(s) 233, 452, 896, 1351
MhlI GDGCHC 2 cut(s) 160, 721
MlsI TGGCCA 1 cut(s) 432
MluNI TGGCCA 1 cut(s) 432
MlyI GAGTC 6 cut(s) 323, 347, 356, 883, 1286, 1660
MmeI TCCRAC 2 cut(s) 283, 375
Mox20I TGGCCA 1 cut(s) 432
Mph1103I ATGCAT 1 cut(s) 1393
MroXI GAANNNNTTC 2 cut(s) 642, 947
MscI TGGCCA 1 cut(s) 432
MseI TTAA 9 cut(s) 36, 603, 660, 713, 777, 927, 1275, 1341, 1553
MslI CAYNNNNRTG 4 cut(s) 269, 288, 384, 591
Msp20I TGGCCA 1 cut(s) 432
MspA1I CMGCKG 1 cut(s) 1133
MspI CCGG 1 cut(s) 1586
MspR9I CCNGG 5 cut(s) 28, 852, 894, 1232, 1562
MunI CAATTG 1 cut(s) 411
MvaI CCWGG 5 cut(s) 28, 852, 894, 1232, 1562
MvnI CGCG 3 cut(s) 43, 162, 559
MwoI GCNNNNNNNGC 2 cut(s) 748, 1057
NcoI CCATGG 1 cut(s) 76
NdeI CATATG 1 cut(s) 195
NlaIII CATG 5 cut(s) 80, 541, 870, 1330, 1549
NlaIV GGNNCC 6 cut(s) 109, 130, 281, 684, 732, 1443
NmuCI GTSAC 2 cut(s) 966, 1657
NsiI ATGCAT 1 cut(s) 1393
PaqCI CACCTGC 1 cut(s) 979
PcsI WCGNNNNNNNCGW 1 cut(s) 444
PdmI GAANNNNTTC 2 cut(s) 642, 947
PfeI GAWTC 4 cut(s) 293, 629, 910, 1508
PfoI TCCNGGA 3 cut(s) 26, 892, 1230
PkrI GCNGC 1 cut(s) 1135
PleI GAGTC 6 cut(s) 323, 347, 355, 883, 1286, 1659
PpsI GAGTC 6 cut(s) 323, 347, 355, 883, 1286, 1659
PpuMI RGGWCCY 1 cut(s) 1442
PsiI TTATAA 1 cut(s) 1437
Psp124BI GAGCTC 1 cut(s) 160
Psp5II RGGWCCY 1 cut(s) 1442
Psp6I CCWGG 5 cut(s) 26, 850, 892, 1230, 1560
PspFI CCCAGC 1 cut(s) 1047
PspGI CCWGG 5 cut(s) 26, 850, 892, 1230, 1560
PspN4I GGNNCC 6 cut(s) 109, 130, 281, 684, 732, 1443
PspPI GGNCC 3 cut(s) 583, 731, 1442
PspPPI RGGWCCY 1 cut(s) 1442
PsuI RGATCY 4 cut(s) 233, 452, 896, 1351
RsaI GTAC 4 cut(s) 109, 446, 684, 1302
RsaNI GTAC 4 cut(s) 108, 445, 683, 1301
RseI CAYNNNNRTG 4 cut(s) 269, 288, 384, 591
SacI GAGCTC 1 cut(s) 160
SaqAI TTAA 9 cut(s) 36, 603, 660, 713, 777, 927, 1275, 1341, 1553
SatI GCNGC 1 cut(s) 1134
Sau96I GGNCC 3 cut(s) 583, 731, 1442
SchI GAGTC 6 cut(s) 323, 347, 356, 883, 1286, 1660
ScrFI CCNGG 5 cut(s) 28, 852, 894, 1232, 1562
SduI GDGCHC 2 cut(s) 160, 721
SfaNI GCATC 4 cut(s) 139, 784, 1060, 1248
SinI GGWCC 3 cut(s) 583, 731, 1442
SmiMI CAYNNNNRTG 4 cut(s) 269, 288, 384, 591
SmlI CTYRAG 1 cut(s) 1055
SmoI CTYRAG 1 cut(s) 1055
SsiI CCGC 4 cut(s) 43, 126, 557, 1131
SspI AATATT 1 cut(s) 577
SspMI CTAG 3 cut(s) 246, 846, 1655
SstI GAGCTC 1 cut(s) 160
StyD4I CCNGG 5 cut(s) 26, 850, 892, 1230, 1560
StyI CCWWGG 2 cut(s) 76, 1144
TaaI ACNGT 3 cut(s) 653, 986, 1116
TaiI ACGT 3 cut(s) 257, 628, 1525
TaqI TCGA 7 cut(s) 251, 327, 622, 676, 1461, 1493, 1506
TaqII GACCGA 1 cut(s) 1346
TfiI GAWTC 4 cut(s) 293, 629, 910, 1508
Tru1I TTAA 9 cut(s) 36, 603, 660, 713, 777, 927, 1275, 1341, 1553
Tru9I TTAA 9 cut(s) 36, 603, 660, 713, 777, 927, 1275, 1341, 1553
TscAI CASTG 1 cut(s) 281
TseFI GTSAC 2 cut(s) 966, 1657
TseI GCWGC 1 cut(s) 1133
Tsp45I GTSAC 2 cut(s) 966, 1657
TspDTI ATGAA 7 cut(s) 182, 378, 501, 526, 651, 1275, 1463
TspGWI ACGGA 5 cut(s) 396, 923, 1083, 1280, 1548
TspRI CASTG 1 cut(s) 281
VneI GTGCAC 1 cut(s) 717
VpaK11BI GGWCC 3 cut(s) 583, 731, 1442
XagI CCTNNNNNAGG 1 cut(s) 849
XapI RAATTY 4 cut(s) 365, 642, 1201, 1536
XmiI GTMKAC 1 cut(s) 376
XmnI GAANNNNTTC 2 cut(s) 642, 947
XspI CTAG 3 cut(s) 246, 846, 1655
Zsp2I ATGCAT 1 cut(s) 1393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.