Rroxscaffold_2G00083790

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005242
Physical Location & Seq
Forward (+)
6403944 .. 6409081
5138 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00083790.1

Sequence Viewer

Length: 1233 bp
ATGGGTGGTTGTGTGTCAACCCCATCTAAAACAATTAAATCACAGAAAAAAAGCCGTAGGGGGAGCAAATTCCATGGAAAGATACATGGTACTAAGAAGAAGAATAGTGATGGAGGAGCTCGAGTAGCAGACTATTCTGTCAGCGAGTTTGTTCATATGGGGTTTGAGAATGGTGCAACAGCTACTTGCACAAGGTCCAAGGTTTCTAATTCAAAATTCCATGTCACAGAGCTGCAATGGCACCACACTCCATCTAATGCGAATGATAGTTTTCCATTAGTGGGCAATGCAATTGGGAATATCCCTGGTGGTCAAGTACTTCAGTATGAAAGGTCTTCATGCTTTGTTGATCATGGGTGCAAGTATGAAGAATACCAGAGCTATACAAAAATAGATGGAGGTAAACTCATGGGCAAAGATGAACACAGGGATTCAGATCGGTTTCCCCGTACTAGTATCCAGGGCTTAGACAGGAAGAAGATGCTAGACCAATCTCGTGGAAGCTTTAAAGGACTGAGAGAGGATCGACGTGATTCTAATGAGAAATCTGGCTTAGCTCGATTAATACATTCTCTAAGTTTCAATGATAAGAATCTACATGCACAAACACACTCTCAAAGAAAGTCATCAACAGTTTTCAGGGTTTCTTTTAAGAGGAGGTCCTGTGATGCAGAAGAAAACAATGAACAATCTGCCATGTTCCTTGGTGATAGTGATGGAGAGGGGATGAGTCTTGTACTGTATTTTAAAGTTTCAGAGAATTTTGATAGAGACATCTCTCCCCAGTTCCAGGAAAGCATCAAGAAAATGGTTGAAGATGAAACAGAAAAGATCAAAGGATTTGCAAAGGATTCGACTGTACCTTTTAGAGAAAGGCTAAAGATTTTGGCTGGGGTGGTTAATCCTGATGAACTCAATCTGAGTTCTGCCGAAAAGAAGCTTTTACATGCATATAATGATAAACCAGTACTTTCACGCCCCCAGCACGAATTTTATAGGGGTCCTAATTATTTTGAGATTGATCTGGACATTCATCGATTCAGCTACCTTCCTCGGAAGGGACTTGAATCTTTTAGAGAACGTCTGCAAAATGGAATACTAGATATGGGTTTAACTATCCAGGCACAGAAACAGGAAGAGTTACCGGAGCAAGTGCTGTGCTGTATGAGACTGAACAAGATTGATTTTGTTGATCATGGTCAAATACCAAGTCTGGTCACTAATGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

410

Amino Acids

46.56

Weight (kDa)

8.76

Isoelectric Point (pI)

43.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 198 - 395 1.1e-39 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 137
AccB1I GGYRCC 1 cut(s) 240
AclWI GGATC 1 cut(s) 531
AcsI RAATTY 4 cut(s) 68, 215, 760, 989
AcuI CTGAAG 1 cut(s) 305
AfaI GTAC 6 cut(s) 91, 318, 451, 738, 861, 969
AfiI CCNNNNNNNGG 3 cut(s) 281, 790, 1058
AgsI TTSAA 4 cut(s) 213, 583, 815, 1067
AhlI ACTAGT 1 cut(s) 452
AjiI CACGTC 1 cut(s) 530
AjnI CCWGG 4 cut(s) 304, 459, 789, 1119
AluBI AGCT 8 cut(s) 119, 182, 232, 381, 504, 557, 940, 1044
AluI AGCT 8 cut(s) 119, 182, 232, 381, 504, 557, 940, 1044
Alw21I GWGCWC 1 cut(s) 121
Alw26I GTCTC 2 cut(s) 765, 1162
AlwI GGATC 1 cut(s) 531
Ama87I CYCGRG 1 cut(s) 120
ApeKI GCWGC 1 cut(s) 232
ApoI RAATTY 4 cut(s) 68, 215, 760, 989
ArsI GACNNNNNNTTYG 2 cut(s) 1195, 1227
AseI ATTAAT 1 cut(s) 563
AspS9I GGNCC 3 cut(s) 195, 660, 1001
AsuHPI GGTGA 1 cut(s) 719
AvaI CYCGRG 1 cut(s) 120
AvaII GGWCC 3 cut(s) 195, 660, 1001
BanI GGYRCC 1 cut(s) 240
BanII GRGCYC 1 cut(s) 121
BauI CACGAG 1 cut(s) 495
BbsI GAAGAC 1 cut(s) 327
Bbv12I GWGCWC 1 cut(s) 121
BbvI GCAGC 1 cut(s) 219
BccI CCATC 5 cut(s) 31, 104, 259, 389, 710
BceAI ACGGC 1 cut(s) 39
BcgI CGANNNNNNTGC 2 cut(s) 834, 868
BciT130I CCWGG 4 cut(s) 306, 461, 791, 1121
BciVI GTATCC 1 cut(s) 467
BclI TGATCA 2 cut(s) 349, 1192
BcoDI GTCTC 2 cut(s) 765, 1162
BcuI ACTAGT 1 cut(s) 452
BfaI CTAG 3 cut(s) 453, 485, 1100
BfuI GTATCC 1 cut(s) 467
BisI GCNGC 1 cut(s) 233
BlpI GCTNAGC 1 cut(s) 553
BlsI GCNGC 1 cut(s) 234
BmcAI AGTACT 2 cut(s) 318, 969
Bme1390I CCNGG 4 cut(s) 306, 461, 791, 1121
Bme18I GGWCC 3 cut(s) 195, 660, 1001
BmeT110I CYCGRG 1 cut(s) 120
BmgBI CACGTC 1 cut(s) 530
BmgT120I GGNCC 3 cut(s) 195, 660, 1001
BmiI GGNNCC 2 cut(s) 242, 1002
BmrFI CCNGG 4 cut(s) 306, 461, 791, 1121
BmrI ACTGGG 1 cut(s) 778
BmsI GCATC 3 cut(s) 471, 658, 807
BmuI ACTGGG 1 cut(s) 778
BpiI GAAGAC 1 cut(s) 327
BplI GAGNNNNNCTC 2 cut(s) 390, 422
Bpu1102I GCTNAGC 1 cut(s) 553
Bsa29I ATCGAT 1 cut(s) 1036
BsaBI GATNNNNATC 2 cut(s) 435, 591
BsaJI CCNNGG 6 cut(s) 73, 198, 304, 460, 703, 1052
BsaWI WCCGGW 1 cut(s) 1144
BsaXI ACNNNNNCTCC 4 cut(s) 108, 138, 649, 679
Bsc4I CCNNNNNNNGG 3 cut(s) 281, 790, 1058
Bse1I ACTGG 2 cut(s) 784, 965
Bse3DI GCAATG 2 cut(s) 242, 292
Bse8I GATNNNNATC 2 cut(s) 435, 591
BseBI CCWGG 4 cut(s) 306, 461, 791, 1121
BseCI ATCGAT 1 cut(s) 1036
BseDI CCNNGG 6 cut(s) 73, 198, 304, 460, 703, 1052
BseGI GGATG 1 cut(s) 732
BseJI GATNNNNATC 2 cut(s) 435, 591
BseLI CCNNNNNNNGG 3 cut(s) 281, 790, 1058
BseMI GCAATG 2 cut(s) 242, 292
BseMII CTCAG 2 cut(s) 506, 911
BseNI ACTGG 2 cut(s) 784, 965
BseRI GAGGAG 2 cut(s) 129, 670
BseXI GCAGC 1 cut(s) 219
BseYI CCCAGC 2 cut(s) 890, 981
BshNI GGYRCC 1 cut(s) 240
BshVI ATCGAT 1 cut(s) 1036
BsiHKAI GWGCWC 1 cut(s) 121
BsiHKCI CYCGRG 1 cut(s) 120
BsiSI CCGG 1 cut(s) 1145
BslFI GGGAC 1 cut(s) 1074
BslI CCNNNNNNNGG 3 cut(s) 281, 790, 1058
BsmAI GTCTC 2 cut(s) 765, 1162
BsmFI GGGAC 1 cut(s) 1074
BsoBI CYCGRG 1 cut(s) 120
Bsp1286I GDGCHC 1 cut(s) 121
Bsp143I GATC 6 cut(s) 349, 436, 523, 831, 1021, 1192
Bsp1720I GCTNAGC 1 cut(s) 553
Bsp19I CCATGG 1 cut(s) 73
BspCNI CTCAG 2 cut(s) 507, 912
BspDI ATCGAT 1 cut(s) 1036
BspLI GGNNCC 2 cut(s) 242, 1002
BspPI GGATC 1 cut(s) 531
BspT107I GGYRCC 1 cut(s) 240
BsrDI GCAATG 2 cut(s) 242, 292
BsrI ACTGG 2 cut(s) 784, 965
BssECI CCNNGG 6 cut(s) 73, 198, 304, 460, 703, 1052
BssMI GATC 6 cut(s) 349, 436, 523, 831, 1021, 1192
BssSI CACGAG 1 cut(s) 495
BssT1I CCWWGG 3 cut(s) 73, 198, 703
Bst2BI CACGAG 1 cut(s) 495
Bst2UI CCWGG 4 cut(s) 306, 461, 791, 1121
Bst4CI ACNGT 3 cut(s) 634, 741, 859
Bst6I CTCTTC 1 cut(s) 1131
BstDEI CTNAG 6 cut(s) 93, 466, 515, 553, 575, 920
BstDSI CCRYGG 1 cut(s) 73
BstF5I GGATG 1 cut(s) 732
BstKTI GATC 6 cut(s) 352, 439, 526, 834, 1024, 1195
BstMAI GTCTC 2 cut(s) 765, 1162
BstMBI GATC 6 cut(s) 349, 436, 523, 831, 1021, 1192
BstMWI GCNNNNNNNGC 2 cut(s) 125, 238
BstNI CCWGG 4 cut(s) 306, 461, 791, 1121
BstNSI RCATGY 2 cut(s) 602, 950
BstSCI CCNGG 4 cut(s) 304, 459, 789, 1119
BstV1I GCAGC 1 cut(s) 219
BstV2I GAAGAC 1 cut(s) 327
BstXI CCANNNNNNTGG 1 cut(s) 497
Bsu15I ATCGAT 1 cut(s) 1036
BsuI GTATCC 1 cut(s) 467
BsuTUI ATCGAT 1 cut(s) 1036
BtgI CCRYGG 1 cut(s) 73
BtrI CACGTC 1 cut(s) 530
BtsCI GGATG 1 cut(s) 732
Cfr13I GGNCC 3 cut(s) 195, 660, 1001
ClaI ATCGAT 1 cut(s) 1036
Csp6I GTAC 6 cut(s) 90, 317, 450, 737, 860, 968
CviQI GTAC 6 cut(s) 90, 317, 450, 737, 860, 968
DdeI CTNAG 6 cut(s) 93, 466, 515, 553, 575, 920
DpnI GATC 6 cut(s) 351, 438, 525, 833, 1023, 1194
DpnII GATC 6 cut(s) 349, 436, 523, 831, 1021, 1192
DraI TTTAAA 2 cut(s) 508, 748
DrdI GACNNNNNNGTC 1 cut(s) 137
DseDI GACNNNNNNGTC 1 cut(s) 137
Eam1104I CTCTTC 1 cut(s) 1131
EarI CTCTTC 1 cut(s) 1131
Ecl136II GAGCTC 1 cut(s) 119
Eco130I CCWWGG 3 cut(s) 73, 198, 703
Eco24I GRGCYC 1 cut(s) 121
Eco47I GGWCC 3 cut(s) 195, 660, 1001
Eco53kI GAGCTC 1 cut(s) 119
Eco57I CTGAAG 1 cut(s) 305
Eco88I CYCGRG 1 cut(s) 120
EcoICRI GAGCTC 1 cut(s) 119
EcoO109I RGGNCCY 2 cut(s) 660, 1001
EcoRII CCWGG 4 cut(s) 304, 459, 789, 1119
EcoT14I CCWWGG 3 cut(s) 73, 198, 703
EcoT22I ATGCAT 1 cut(s) 952
EcoT38I GRGCYC 1 cut(s) 121
ErhI CCWWGG 3 cut(s) 73, 198, 703
FaqI GGGAC 1 cut(s) 1074
FauNDI CATATG 1 cut(s) 156
FbaI TGATCA 2 cut(s) 349, 1192
Fnu4HI GCNGC 1 cut(s) 233
FokI GGATG 1 cut(s) 739
FriOI GRGCYC 1 cut(s) 121
Fsp4HI GCNGC 1 cut(s) 233
FspBI CTAG 3 cut(s) 453, 485, 1100
GluI GCNGC 1 cut(s) 233
GsaI CCCAGC 2 cut(s) 894, 985
HapII CCGG 1 cut(s) 1145
HincII GTYRAC 1 cut(s) 18
HindII GTYRAC 1 cut(s) 18
HindIII AAGCTT 2 cut(s) 502, 938
HinfI GANTC 7 cut(s) 431, 533, 592, 730, 851, 1038, 1067
HpaII CCGG 1 cut(s) 1145
HphI GGTGA 1 cut(s) 719
Hpy166II GTNNAC 2 cut(s) 18, 404
Hpy188I TCNGA 4 cut(s) 436, 757, 921, 1056
Hpy188III TCNNGA 3 cut(s) 802, 905, 1025
Hpy8I GTNNAC 2 cut(s) 18, 404
Hpy99I CGWCG 1 cut(s) 531
HpyAV CCTTC 2 cut(s) 1051, 1058
HpyCH4III ACNGT 3 cut(s) 634, 741, 859
HpyCH4IV ACGT 2 cut(s) 529, 1081
HpyF10VI GCNNNNNNNGC 2 cut(s) 125, 238
HpyF3I CTNAG 6 cut(s) 93, 466, 515, 553, 575, 920
HpySE526I ACGT 2 cut(s) 529, 1081
Ksp22I TGATCA 2 cut(s) 349, 1192
Kzo9I GATC 6 cut(s) 349, 436, 523, 831, 1021, 1192
LmnI GCTCC 3 cut(s) 63, 116, 1147
Lsp1109I GCAGC 1 cut(s) 219
LweI GCATC 3 cut(s) 471, 658, 807
MaeI CTAG 3 cut(s) 453, 485, 1100
MaeII ACGT 2 cut(s) 529, 1081
MaeIII GTNAC 3 cut(s) 223, 1140, 1216
MalI GATC 6 cut(s) 351, 438, 525, 833, 1023, 1194
MboI GATC 6 cut(s) 349, 436, 523, 831, 1021, 1192
MboII GAAGA 9 cut(s) 109, 112, 327, 380, 487, 490, 686, 827, 1148
MfeI CAATTG 1 cut(s) 291
MhlI GDGCHC 1 cut(s) 121
MluCI AATT 8 cut(s) 33, 68, 208, 215, 291, 760, 989, 1006
MlyI GAGTC 1 cut(s) 739
MnlI CCTC 7 cut(s) 107, 392, 514, 648, 651, 715, 1062
Mph1103I ATGCAT 1 cut(s) 952
MseI TTAA 7 cut(s) 36, 507, 563, 651, 747, 900, 1112
MspI CCGG 1 cut(s) 1145
MspR9I CCNGG 4 cut(s) 306, 461, 791, 1121
MunI CAATTG 1 cut(s) 291
MvaI CCWGG 4 cut(s) 306, 461, 791, 1121
MwoI GCNNNNNNNGC 2 cut(s) 125, 238
NcoI CCATGG 1 cut(s) 73
NdeI CATATG 1 cut(s) 156
NdeII GATC 6 cut(s) 349, 436, 523, 831, 1021, 1192
NlaIV GGNNCC 2 cut(s) 242, 1002
NmuCI GTSAC 2 cut(s) 223, 1216
NsiI ATGCAT 1 cut(s) 952
NspI RCATGY 2 cut(s) 602, 950
PaeR7I CTCGAG 1 cut(s) 120
PcsI WCGNNNNNNNCGW 1 cut(s) 445
PfeI GAWTC 6 cut(s) 431, 533, 592, 851, 1038, 1067
PfoI TCCNGGA 1 cut(s) 789
PkrI GCNGC 1 cut(s) 234
PleI GAGTC 1 cut(s) 738
PpsI GAGTC 1 cut(s) 738
PpuMI RGGWCCY 2 cut(s) 660, 1001
PshBI ATTAAT 1 cut(s) 563
Psp124BI GAGCTC 1 cut(s) 121
Psp5II RGGWCCY 2 cut(s) 660, 1001
Psp6I CCWGG 4 cut(s) 304, 459, 789, 1119
PspFI CCCAGC 2 cut(s) 890, 981
PspGI CCWGG 4 cut(s) 304, 459, 789, 1119
PspN4I GGNNCC 2 cut(s) 242, 1002
PspPI GGNCC 3 cut(s) 195, 660, 1001
PspPPI RGGWCCY 2 cut(s) 660, 1001
PspXI VCTCGAGB 1 cut(s) 120
RsaI GTAC 6 cut(s) 91, 318, 451, 738, 861, 969
RsaNI GTAC 6 cut(s) 90, 317, 450, 737, 860, 968
SacI GAGCTC 1 cut(s) 121
SaqAI TTAA 7 cut(s) 36, 507, 563, 651, 747, 900, 1112
SatI GCNGC 1 cut(s) 233
Sau3AI GATC 6 cut(s) 349, 436, 523, 831, 1021, 1192
Sau96I GGNCC 3 cut(s) 195, 660, 1001
ScaI AGTACT 2 cut(s) 318, 969
SchI GAGTC 1 cut(s) 739
ScrFI CCNGG 4 cut(s) 306, 461, 791, 1121
SduI GDGCHC 1 cut(s) 121
SfaNI GCATC 3 cut(s) 471, 658, 807
Sfr274I CTCGAG 1 cut(s) 120
SinI GGWCC 3 cut(s) 195, 660, 1001
SlaI CTCGAG 1 cut(s) 120
SmlI CTYRAG 1 cut(s) 120
SmoI CTYRAG 1 cut(s) 120
SpeI ACTAGT 1 cut(s) 452
Sse9I AATT 8 cut(s) 33, 68, 208, 215, 291, 760, 989, 1006
SspMI CTAG 3 cut(s) 453, 485, 1100
SstI GAGCTC 1 cut(s) 121
StyD4I CCNGG 4 cut(s) 304, 459, 789, 1119
StyI CCWWGG 3 cut(s) 73, 198, 703
TaaI ACNGT 3 cut(s) 634, 741, 859
TaiI ACGT 2 cut(s) 532, 1084
TaqI TCGA 5 cut(s) 121, 526, 559, 854, 1036
TasI AATT 8 cut(s) 33, 68, 208, 215, 291, 760, 989, 1006
TatI WGTACW 3 cut(s) 316, 736, 967
TfiI GAWTC 6 cut(s) 431, 533, 592, 851, 1038, 1067
Tru1I TTAA 7 cut(s) 36, 507, 563, 651, 747, 900, 1112
Tru9I TTAA 7 cut(s) 36, 507, 563, 651, 747, 900, 1112
TseFI GTSAC 2 cut(s) 223, 1216
TseI GCWGC 1 cut(s) 232
Tsp45I GTSAC 2 cut(s) 223, 1216
TspDTI ATGAA 9 cut(s) 143, 327, 342, 381, 435, 699, 834, 924, 1022
VpaK11BI GGWCC 3 cut(s) 195, 660, 1001
VspI ATTAAT 1 cut(s) 563
XapI RAATTY 4 cut(s) 68, 215, 760, 989
XceI RCATGY 2 cut(s) 602, 950
XhoI CTCGAG 1 cut(s) 120
XspI CTAG 3 cut(s) 453, 485, 1100
ZrmI AGTACT 2 cut(s) 318, 969
Zsp2I ATGCAT 1 cut(s) 952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.