MD09G1056400.v1.1

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005242
Physical Location & Seq
Forward (+)
3731315 .. 3734042
2728 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1056400.v1.1.491

Sequence Viewer

Length: 1656 bp
ATGGGTGGTTGTGTGTCAACCCCGTCCAGAACAATTAAATCACGGAAGAAACACCGTCAGAGGGTCAACAAACGCCATGGAAAGATAACCTGTTCTGCTTCTGATGGTACCAAGAAGAGAAACAGCGATGCAGGAGCTCGCGTAACTGATTATTCTGTGAGTGAGTTTGTTCTTGAGGACTTTGAGAATGGTGCAACAACTACTCACAGGAGATCTAAGGTTTCTAATTCTACGTTCCAACTCACCCAGATGCAGTGGCACCGCCAATATGATTCGAATGTAATTTGCCAAGAAGAAACTTGGTTCGACTCAGTGAGTATTCTGGAATCCGACTCGGACGATGAATTTATCAGTGTACACGGAGATGGTTTTCCATTAGCAGGCAATCCCATTGGGAATATCTCAGGCGGCCAAGTAGTTCAGTTTGAAAGATCTGCACACTTTGTAGATAACGGGTGTAAGTATGAAGAATACCAAAGCTATACAAAAATAGATGGAGGTAAATCGGATAAGTTCATGGGGAAGGATGACGAAGTTCGTAGCAAGAGGAAGAATATTCTGGATCATTCTTATGGAAGCTTTAAAGGACTTAGAGAGGATCGACGTGATTCTAATGAAAAAATTCAAGGCAATATGCTTAAGTCTGCCTTGCCTCGAATGATACCTTCTATAAGTTTCAACGATAAGATATTAAGTGCGCAGAGTTTGGCTCCACAGGCTCAAAGAAAGCCATCAGCAGTTTTCAGGCTTTCTTTTAAGAGGAGATCATGTGATGCGGACGAAACCATTGAACAATGTCAATCAAAAAGGTTTCTGTATCGTCCCAGACCAGGATATATCATTCCATGTTGTAGAGTAGAGAAGCTGACTTCAGGATCTTGGTCTGAGATTCCACCCTCAACTTTCAAACTCCGTGGCGAGAACTATTTCAATGATAAACGGAAGTCACCTGCTCCAAACTACAGTCCATATACTCCAATAGGTATTGATTTATTTGTGTGCCCCAAGAAGATACACCACATTGCCCAGCATCTTGAGCTTCCCAAAGTAAAAGCCAACGGAAAAGTGCCTTCACTCCTCATTGTAAATATACAGTTGCCTACTTATCCCACTGCAATGTTCCTCGGTGATAGCGATGGAGAGGGGATGAGTCTCGTAATGTATTTCAAAATTTCTGAAAGTTTTGAGAAAGACATCTCTCCTCAATTCCAGGACAGCATCAAGAAATTGGTTGACGATGAAATGGAAAAGGTTAAAGGATTTGCAAAGGACTCAATTGTACCTTTTAGAGAAAGGCTAAAGATCATGGCTGGGGTGGTTAATCCTGAGGATCTCGGTCTGAGTTCTGCTGAAAAGAAGCTTGTAAATGCATATAACGAAAAACCAGTTCTTTCGCGTCCTCAGCACAGTTTTTATAAGGGCCCTAATTACTTTGAGATTGATCTGGACATTCATCGCTTCAGCTACATATCGAGGAAGGGTCTTGAATCCTTTAGAGAGCGTCTGAAAAACGGAATTCTGGACATGGGTTTGACCATCCAGGCACAAAAACAGGAAGAACTACCGGAGCAAGTGCTGTGTTGTATGAGGCTGAACAAGATCGATTTTGTTGATCACGGCCAAATACCGAGTCTAGTGACCGTTGAGGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

552

Amino Acids

62.61

Weight (kDa)

8.8

Isoelectric Point (pI)

45.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 294 - 535 7.4e-65 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1416
AarI CACCTGC 1 cut(s) 958
Acc16I TGCGCA 1 cut(s) 699
Acc36I ACCTGC 1 cut(s) 958
Acc65I GGTACC 1 cut(s) 107
AccB1I GGYRCC 2 cut(s) 107, 258
AccII CGCG 2 cut(s) 141, 1396
AciI CCGC 3 cut(s) 262, 408, 776
AclWI GGATC 4 cut(s) 570, 606, 883, 1338
AcoI YGGCCR 2 cut(s) 409, 1618
AcsI RAATTY 4 cut(s) 344, 621, 1170, 1515
AcuI CTGAAG 2 cut(s) 855, 1444
AfaI GTAC 3 cut(s) 109, 357, 1281
AfiI CCNNNNNNNGG 3 cut(s) 61, 380, 830
AflII CTTAAG 1 cut(s) 638
AgsI TTSAA 8 cut(s) 428, 626, 679, 791, 907, 931, 1168, 1487
AjiI CACGTC 1 cut(s) 605
AjnI CCWGG 3 cut(s) 829, 1209, 1539
AluBI AGCT 7 cut(s) 137, 480, 579, 865, 1039, 1360, 1464
AluI AGCT 7 cut(s) 137, 480, 579, 865, 1039, 1360, 1464
Alw21I GWGCWC 1 cut(s) 139
Alw26I GTCTC 1 cut(s) 1157
AlwI GGATC 4 cut(s) 570, 606, 883, 1338
AoxI GGCC 3 cut(s) 409, 1420, 1618
ApaI GGGCCC 1 cut(s) 1424
ApoI RAATTY 4 cut(s) 344, 621, 1170, 1515
ArsI GACNNNNNNTTYG 2 cut(s) 1615, 1647
Asp700I GAANNNNTTC 2 cut(s) 621, 926
Asp718I GGTACC 1 cut(s) 107
AspLEI GCGC 1 cut(s) 700
AspS9I GGNCC 2 cut(s) 1420, 1421
AsuHPI GGTGA 3 cut(s) 235, 939, 1139
AsuII TTCGAA 1 cut(s) 275
AxyI CCTNAGG 1 cut(s) 1326
BaeGI GKGCMC 2 cut(s) 1004, 1424
BanI GGYRCC 2 cut(s) 107, 258
BanII GRGCYC 2 cut(s) 139, 1424
Bbv12I GWGCWC 1 cut(s) 139
BbvCI CCTCAGC 1 cut(s) 1401
BccI CCATC 6 cut(s) 98, 359, 488, 739, 1130, 1544
BceAI ACGGC 1 cut(s) 1633
BciT130I CCWGG 3 cut(s) 831, 1211, 1541
BclI TGATCA 1 cut(s) 1612
BcoDI GTCTC 1 cut(s) 1157
BfaI CTAG 1 cut(s) 1634
BfmI CTRYAG 1 cut(s) 961
BfrI CTTAAG 1 cut(s) 638
BfuAI ACCTGC 1 cut(s) 958
BglII AGATCT 2 cut(s) 212, 431
BisI GCNGC 1 cut(s) 409
BlsI GCNGC 1 cut(s) 410
Bme1390I CCNGG 3 cut(s) 831, 1211, 1541
BmgBI CACGTC 1 cut(s) 605
BmgT120I GGNCC 2 cut(s) 1420, 1421
BmiI GGNNCC 4 cut(s) 109, 260, 711, 1422
BmrFI CCNGG 3 cut(s) 831, 1211, 1541
BmsI GCATC 5 cut(s) 118, 240, 763, 1039, 1227
BplI GAGNNNNNCTC 2 cut(s) 694, 726
Bpu10I CCTNAGC 1 cut(s) 1401
Bpu14I TTCGAA 1 cut(s) 275
BpuEI CTTGAG 2 cut(s) 194, 1055
Bsa29I ATCGAT 1 cut(s) 1602
BsaJI CCNNGG 3 cut(s) 76, 913, 1123
BsaWI WCCGGW 1 cut(s) 1564
BsaXI ACNNNNNCTCC 4 cut(s) 126, 156, 754, 784
Bsc4I CCNNNNNNNGG 3 cut(s) 61, 380, 830
Bse1I ACTGG 1 cut(s) 1385
Bse21I CCTNAGG 1 cut(s) 1326
Bse3DI GCAATG 2 cut(s) 1020, 1122
BseBI CCWGG 3 cut(s) 831, 1211, 1541
BseCI ATCGAT 1 cut(s) 1602
BseDI CCNNGG 3 cut(s) 76, 913, 1123
BseGI GGATG 4 cut(s) 532, 1152, 1536, 1654
BseLI CCNNNNNNNGG 3 cut(s) 61, 380, 830
BseMI GCAATG 2 cut(s) 1020, 1122
BseMII CTCAG 6 cut(s) 324, 417, 876, 1317, 1331, 1415
BseNI ACTGG 1 cut(s) 1385
BseRI GAGGAG 3 cut(s) 775, 1067, 1191
BseSI GKGCMC 2 cut(s) 1004, 1424
BseYI CCCAGC 2 cut(s) 1026, 1310
BsgI GTGCAG 1 cut(s) 420
Bsh1236I CGCG 2 cut(s) 141, 1396
BshFI GGCC 3 cut(s) 411, 1422, 1620
BshNI GGYRCC 2 cut(s) 107, 258
BshVI ATCGAT 1 cut(s) 1602
BsiHKAI GWGCWC 1 cut(s) 139
BsiSI CCGG 1 cut(s) 1565
BslFI GGGAC 1 cut(s) 807
BslI CCNNNNNNNGG 3 cut(s) 61, 380, 830
BsmAI GTCTC 1 cut(s) 1157
BsmFI GGGAC 1 cut(s) 807
BsnI GGCC 3 cut(s) 411, 1422, 1620
Bsp119I TTCGAA 1 cut(s) 275
Bsp120I GGGCCC 1 cut(s) 1420
Bsp1286I GDGCHC 3 cut(s) 139, 1004, 1424
Bsp1407I TGTACA 1 cut(s) 355
Bsp19I CCATGG 1 cut(s) 76
BspACI CCGC 3 cut(s) 262, 408, 776
BspANI GGCC 3 cut(s) 411, 1422, 1620
BspCNI CTCAG 6 cut(s) 323, 416, 877, 1318, 1332, 1414
BspDI ATCGAT 1 cut(s) 1602
BspFNI CGCG 2 cut(s) 141, 1396
BspLI GGNNCC 4 cut(s) 109, 260, 711, 1422
BspMI ACCTGC 1 cut(s) 958
BspPI GGATC 4 cut(s) 570, 606, 883, 1338
BspT104I TTCGAA 1 cut(s) 275
BspT107I GGYRCC 2 cut(s) 107, 258
BspTI CTTAAG 1 cut(s) 638
BsrDI GCAATG 2 cut(s) 1020, 1122
BsrGI TGTACA 1 cut(s) 355
BsrI ACTGG 1 cut(s) 1385
BssECI CCNNGG 3 cut(s) 76, 913, 1123
BssT1I CCWWGG 1 cut(s) 76
Bst2UI CCWGG 3 cut(s) 831, 1211, 1541
Bst4CI ACNGT 5 cut(s) 56, 965, 1095, 1409, 1642
Bst6I CTCTTC 1 cut(s) 110
BstAFI CTTAAG 1 cut(s) 638
BstAUI TGTACA 1 cut(s) 355
BstBI TTCGAA 1 cut(s) 275
BstC8I GCNNGC 2 cut(s) 139, 382
BstDEI CTNAG 8 cut(s) 216, 310, 403, 590, 885, 1326, 1340, 1401
BstDSI CCRYGG 2 cut(s) 76, 913
BstF5I GGATG 4 cut(s) 532, 1152, 1536, 1654
BstFNI CGCG 2 cut(s) 141, 1396
BstHHI GCGC 1 cut(s) 700
BstMAI GTCTC 1 cut(s) 1157
BstMWI GCNNNNNNNGC 3 cut(s) 716, 1036, 1402
BstNI CCWGG 3 cut(s) 831, 1211, 1541
BstSCI CCNGG 3 cut(s) 829, 1209, 1539
BstSFI CTRYAG 1 cut(s) 961
BstSLI GKGCMC 2 cut(s) 1004, 1424
BstUI CGCG 2 cut(s) 141, 1396
BstX2I RGATCY 4 cut(s) 212, 431, 875, 1330
BstYI RGATCY 4 cut(s) 212, 431, 875, 1330
Bsu15I ATCGAT 1 cut(s) 1602
Bsu36I CCTNAGG 1 cut(s) 1326
BsuRI GGCC 3 cut(s) 411, 1422, 1620
BsuTUI ATCGAT 1 cut(s) 1602
BtgI CCRYGG 2 cut(s) 76, 913
BtgZI GCGATG 3 cut(s) 141, 1149, 1439
BtrI CACGTC 1 cut(s) 605
BtsCI GGATG 4 cut(s) 532, 1152, 1536, 1654
BtsI GCAGTG 2 cut(s) 260, 1110
BtsIMutI CAGTG 4 cut(s) 260, 318, 358, 1110
BveI ACCTGC 1 cut(s) 958
Cac8I GCNNGC 2 cut(s) 139, 382
CfoI GCGC 1 cut(s) 700
Cfr13I GGNCC 2 cut(s) 1420, 1421
ClaI ATCGAT 1 cut(s) 1602
CseI GACGC 2 cut(s) 1385, 1490
Csp6I GTAC 3 cut(s) 108, 356, 1280
CspCI CAANNNNNGTGG 2 cut(s) 895, 930
CviAII CATG 6 cut(s) 77, 517, 768, 846, 1306, 1525
CviQI GTAC 3 cut(s) 108, 356, 1280
DdeI CTNAG 8 cut(s) 216, 310, 403, 590, 885, 1326, 1340, 1401
DraI TTTAAA 1 cut(s) 583
EaeI YGGCCR 2 cut(s) 409, 1618
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
Ecl136II GAGCTC 1 cut(s) 137
Eco130I CCWWGG 1 cut(s) 76
Eco24I GRGCYC 2 cut(s) 139, 1424
Eco53kI GAGCTC 1 cut(s) 137
Eco57I CTGAAG 2 cut(s) 855, 1444
Eco81I CCTNAGG 1 cut(s) 1326
EcoICRI GAGCTC 1 cut(s) 137
EcoO109I RGGNCCY 2 cut(s) 1420, 1421
EcoRI GAATTC 1 cut(s) 1515
EcoRII CCWGG 3 cut(s) 829, 1209, 1539
EcoT14I CCWWGG 1 cut(s) 76
EcoT22I ATGCAT 1 cut(s) 1372
EcoT38I GRGCYC 2 cut(s) 139, 1424
ErhI CCWWGG 1 cut(s) 76
FaeI CATG 6 cut(s) 80, 520, 771, 849, 1309, 1528
FalI AAGNNNNNCTT 2 cut(s) 632, 664
FaqI GGGAC 1 cut(s) 807
FatI CATG 6 cut(s) 76, 516, 767, 845, 1305, 1524
FbaI TGATCA 1 cut(s) 1612
Fnu4HI GCNGC 1 cut(s) 409
FokI GGATG 3 cut(s) 539, 1159, 1523
FriOI GRGCYC 2 cut(s) 139, 1424
Fsp4HI GCNGC 1 cut(s) 409
FspBI CTAG 1 cut(s) 1634
FspI TGCGCA 1 cut(s) 699
GlaI GCGC 1 cut(s) 699
GluI GCNGC 1 cut(s) 409
GsaI CCCAGC 2 cut(s) 1030, 1314
HaeIII GGCC 3 cut(s) 411, 1422, 1620
HapII CCGG 1 cut(s) 1565
HgaI GACGC 2 cut(s) 1385, 1490
HhaI GCGC 1 cut(s) 700
Hin1II CATG 6 cut(s) 80, 520, 771, 849, 1309, 1528
Hin6I GCGC 1 cut(s) 698
HinP1I GCGC 1 cut(s) 698
HincII GTYRAC 3 cut(s) 18, 67, 1234
HindII GTYRAC 3 cut(s) 18, 67, 1234
HindIII AAGCTT 2 cut(s) 577, 1358
HpaII CCGG 1 cut(s) 1565
HphI GGTGA 3 cut(s) 235, 939, 1139
Hpy166II GTNNAC 5 cut(s) 18, 67, 356, 358, 1234
Hpy188I TCNGA 9 cut(s) 60, 103, 331, 337, 508, 886, 1177, 1341, 1506
Hpy8I GTNNAC 5 cut(s) 18, 67, 356, 358, 1234
Hpy99I CGWCG 1 cut(s) 606
HpyAV CCTTC 4 cut(s) 517, 675, 1080, 1471
HpyCH4III ACNGT 5 cut(s) 56, 965, 1095, 1409, 1642
HpyCH4IV ACGT 2 cut(s) 233, 604
HpyCH4V TGCA 7 cut(s) 131, 194, 253, 437, 1115, 1265, 1370
HpyF10VI GCNNNNNNNGC 3 cut(s) 716, 1036, 1402
HpyF3I CTNAG 8 cut(s) 216, 310, 403, 590, 885, 1326, 1340, 1401
HpySE526I ACGT 2 cut(s) 233, 604
Hsp92II CATG 6 cut(s) 80, 520, 771, 849, 1309, 1528
HspAI GCGC 1 cut(s) 698
KpnI GGTACC 1 cut(s) 111
Ksp22I TGATCA 1 cut(s) 1612
LmnI GCTCC 4 cut(s) 134, 715, 958, 1567
LweI GCATC 5 cut(s) 118, 240, 763, 1039, 1227
MaeI CTAG 1 cut(s) 1634
MaeII ACGT 2 cut(s) 233, 604
MaeIII GTNAC 3 cut(s) 142, 945, 1636
MboII GAAGA 7 cut(s) 58, 127, 305, 479, 562, 1021, 1568
MfeI CAATTG 1 cut(s) 1275
MflI RGATCY 4 cut(s) 212, 431, 875, 1330
MhlI GDGCHC 3 cut(s) 139, 1004, 1424
MlyI GAGTC 5 cut(s) 302, 326, 1159, 1265, 1639
MmeI TCCRAC 2 cut(s) 262, 354
Mph1103I ATGCAT 1 cut(s) 1372
MroXI GAANNNNTTC 2 cut(s) 621, 926
MseI TTAA 7 cut(s) 36, 582, 639, 692, 756, 1254, 1320
MslI CAYNNNNRTG 4 cut(s) 248, 363, 570, 1115
MspCI CTTAAG 1 cut(s) 638
MspI CCGG 1 cut(s) 1565
MspR9I CCNGG 3 cut(s) 831, 1211, 1541
MunI CAATTG 1 cut(s) 1275
MvaI CCWGG 3 cut(s) 831, 1211, 1541
MvnI CGCG 2 cut(s) 141, 1396
MwoI GCNNNNNNNGC 3 cut(s) 716, 1036, 1402
NcoI CCATGG 1 cut(s) 76
NlaIII CATG 6 cut(s) 80, 520, 771, 849, 1309, 1528
NlaIV GGNNCC 4 cut(s) 109, 260, 711, 1422
NmuCI GTSAC 2 cut(s) 945, 1636
NsbI TGCGCA 1 cut(s) 699
NsiI ATGCAT 1 cut(s) 1372
NspV TTCGAA 1 cut(s) 275
PaqCI CACCTGC 1 cut(s) 958
PcsI WCGNNNNNNNCGW 1 cut(s) 1131
PdmI GAANNNNTTC 2 cut(s) 621, 926
PfeI GAWTC 5 cut(s) 272, 326, 608, 889, 1487
PfoI TCCNGGA 1 cut(s) 1209
PkrI GCNGC 1 cut(s) 410
PleI GAGTC 5 cut(s) 302, 326, 1158, 1265, 1638
PpsI GAGTC 5 cut(s) 302, 326, 1158, 1265, 1638
PsiI TTATAA 1 cut(s) 1416
Psp124BI GAGCTC 1 cut(s) 139
Psp6I CCWGG 3 cut(s) 829, 1209, 1539
PspFI CCCAGC 2 cut(s) 1026, 1310
PspGI CCWGG 3 cut(s) 829, 1209, 1539
PspN4I GGNNCC 4 cut(s) 109, 260, 711, 1422
PspOMI GGGCCC 1 cut(s) 1420
PspPI GGNCC 2 cut(s) 1420, 1421
PsuI RGATCY 4 cut(s) 212, 431, 875, 1330
RsaI GTAC 3 cut(s) 109, 357, 1281
RsaNI GTAC 3 cut(s) 108, 356, 1280
RseI CAYNNNNRTG 4 cut(s) 248, 363, 570, 1115
SacI GAGCTC 1 cut(s) 139
SaqAI TTAA 7 cut(s) 36, 582, 639, 692, 756, 1254, 1320
SatI GCNGC 1 cut(s) 409
Sau96I GGNCC 2 cut(s) 1420, 1421
SchI GAGTC 5 cut(s) 302, 326, 1159, 1265, 1639
ScrFI CCNGG 3 cut(s) 831, 1211, 1541
SduI GDGCHC 3 cut(s) 139, 1004, 1424
SfaNI GCATC 5 cut(s) 118, 240, 763, 1039, 1227
SfcI CTRYAG 1 cut(s) 961
SfuI TTCGAA 1 cut(s) 275
SmiMI CAYNNNNRTG 4 cut(s) 248, 363, 570, 1115
SmlI CTYRAG 3 cut(s) 173, 638, 1034
SmoI CTYRAG 3 cut(s) 173, 638, 1034
SsiI CCGC 3 cut(s) 262, 408, 776
SspI AATATT 1 cut(s) 556
SspMI CTAG 1 cut(s) 1634
SstI GAGCTC 1 cut(s) 139
StyD4I CCNGG 3 cut(s) 829, 1209, 1539
StyI CCWWGG 1 cut(s) 76
TaaI ACNGT 5 cut(s) 56, 965, 1095, 1409, 1642
TaiI ACGT 2 cut(s) 236, 607
TaqI TCGA 6 cut(s) 275, 306, 601, 655, 1472, 1602
TaqII GACCGA 1 cut(s) 1325
TatI WGTACW 1 cut(s) 355
TauI GCSGC 1 cut(s) 411
TfiI GAWTC 5 cut(s) 272, 326, 608, 889, 1487
Tru1I TTAA 7 cut(s) 36, 582, 639, 692, 756, 1254, 1320
Tru9I TTAA 7 cut(s) 36, 582, 639, 692, 756, 1254, 1320
TscAI CASTG 4 cut(s) 260, 318, 358, 1117
TseFI GTSAC 2 cut(s) 945, 1636
Tsp45I GTSAC 2 cut(s) 945, 1636
TspDTI ATGAA 6 cut(s) 357, 480, 505, 630, 1254, 1442
TspGWI ACGGA 6 cut(s) 58, 375, 902, 955, 1074, 1527
TspRI CASTG 4 cut(s) 260, 318, 358, 1117
Vha464I CTTAAG 1 cut(s) 638
XapI RAATTY 4 cut(s) 344, 621, 1170, 1515
XmnI GAANNNNTTC 2 cut(s) 621, 926
XspI CTAG 1 cut(s) 1634
Zsp2I ATGCAT 1 cut(s) 1372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.