Rw2G049500

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005242
Physical Location & Seq
Reverse (-)
77077853 .. 77082713
4861 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G049500.1

Sequence Viewer

Length: 1632 bp
ATGGGTGGTTGTGTGTCAACCCCATCTAAAACGATTAAATCACAGAAAAAAAGCCGTAGGGGCAGCAAATTCCATGGAAAGATAAACGGTACTAAGAAGAAGAATAGTGATGGAGGAGCTCGAGTAGCAGATTATTCTGTCAGCGAGTTTGTCCATATGGGGTTTGAGAATGGTGCAACAGCTACTTGCAAAAGGTCCAAGGTTTCTAATTCAAAATTCCATCTCACGGAGCTGCAATGGCACCACACTCCATCTAATGCGAATGCCATTTGCCAAGATGAATCTTGGTTTGACTCCGTCAGTGTACTGGAATCTGAATCAGATGACGAATATGTTAGTTTACATGGAGATAGTTTTCCATTATCGGGCAATGCAATTGGGAATATCCCTGGTGGTCAAGTACTTCAGTATGAAAGGTCTTCATGCTTTGTTGATAATGGGTGCAAGTATGAAGAATACCAGAGCTATACAAAAATAGATGGAGGTAAACTCATGGGTAAAGATGAACACAGGGATTCAAATCGGTTTCCCCGTACTAGTATCCAGGGCTTAGACAGGAAGAAGATGCTAGACCAAGCTCGTGGAAGCTTTAAAGGACTGACGGAGGATCGACGTGATTCTAATGAGAAATCTGGCTTAGCTCGATTAATACATTCTCTAAGTTTCAATGATAAGAATCTACATGCACAAACACACTCTCAAAGAAAGCCATCAACAGTTTTCAGGCTTTCTTTTAAGAGGCGGTCCTGTGATGCGGAAGAAAACAATGAACAATATCAATCAAAAAGGTTTCTGAGTCGGCCTAGAGCAGGACTTGTCATTCCCTGTTGTAAAGGAGAGAAGATGACTCCAGGATGTTGGTCTGAGATTCCACCCTCCAATTTCAAACTCCGTGGCGAAAACTATTTCAAAGATAAAAAGAAGGCACCTGCTGCGAACTACAGTCCATATACTCCAATTGGTGTTGATCTATTTGTCTGCCCCAAAAAGATACATCACATTGCTCAATATCTTGAGCTTCCAAAAGTAAATGCAGATGTAAAAGTGCCTCCACTCCTCATTGTAAATATACAGTTGCCTACTTATCCAGCTGCCATGTTCCTTGGTGATAGTGATGGAGAGGGAATGAGTCTTGTACTGTATTTTAAAGTTTCTGAGAATTTTGATAGAGACATCTCTCCCCAATTCCAGGAAAGCATCAAGAAAATGGTTGAAGATGAAACAGAAAAGGTCAAAGGATTTGCAAAGGATTCGACTGTACCTTTTAGAGAAAGGCTAAAGATCTTGGCTGGGGTGGTTAATCCTGATGAACTCAATCTGAGTTCTGCCGAAAAGAAGCTTTTACATGCATATAATGATAAACCAGTACTTTCACGCCCCCAGCACGAATTTTATAGGGGTCCTAATTATTTTGAGATTGATCTGGACATTCATCGGTTCAGCTACCTTCCTCGGAAGGGACTTGAATCTTTTAGAGAACGTCTGCAAAATGGAATCCTAGATATGGGTTTAACTATCCAGGCACAGAAACAGGAAGAGTTGCCGGAGCAAGTGCTGTGCTGTATGAGACTGAACAAGATTGATTTTGTTGATCATGGTCAAATACCAAGTCTGGTCACTAATGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

543

Amino Acids

61.46

Weight (kDa)

8.69

Isoelectric Point (pI)

42.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 287 - 528 3.2e-64 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 937
Acc36I ACCTGC 1 cut(s) 937
AccB1I GGYRCC 2 cut(s) 240, 925
AciI CCGC 2 cut(s) 742, 755
AclWI GGATC 1 cut(s) 615
AcsI RAATTY 4 cut(s) 68, 215, 1159, 1388
AcuI CTGAAG 1 cut(s) 389
AfaI GTAC 7 cut(s) 91, 306, 402, 535, 1137, 1260, 1368
AfiI CCNNNNNNNGG 6 cut(s) 226, 365, 809, 1189, 1457, 1504
AgsI TTSAA 7 cut(s) 213, 519, 667, 886, 910, 1214, 1466
AhlI ACTAGT 1 cut(s) 536
AjiI CACGTC 1 cut(s) 614
AjnI CCWGG 5 cut(s) 388, 543, 850, 1188, 1518
Alw21I GWGCWC 1 cut(s) 121
Alw26I GTCTC 2 cut(s) 1164, 1561
AlwI GGATC 1 cut(s) 615
Ama87I CYCGRG 1 cut(s) 120
AoxI GGCC 1 cut(s) 800
ApeKI GCWGC 4 cut(s) 63, 232, 932, 1091
ApoI RAATTY 4 cut(s) 68, 215, 1159, 1388
ArsI GACNNNNNNTTYG 2 cut(s) 1594, 1626
AseI ATTAAT 1 cut(s) 647
AspS9I GGNCC 3 cut(s) 195, 744, 1400
AsuHPI GGTGA 1 cut(s) 1118
AvaI CYCGRG 1 cut(s) 120
AvaII GGWCC 3 cut(s) 195, 744, 1400
BanI GGYRCC 2 cut(s) 240, 925
BanII GRGCYC 1 cut(s) 121
BauI CACGAG 1 cut(s) 579
BbsI GAAGAC 1 cut(s) 411
Bbv12I GWGCWC 1 cut(s) 121
BbvI GCAGC 4 cut(s) 75, 219, 919, 1078
BccI CCATC 7 cut(s) 31, 104, 228, 259, 473, 718, 1109
BceAI ACGGC 1 cut(s) 39
BcgI CGANNNNNNTGC 2 cut(s) 1233, 1267
BciT130I CCWGG 5 cut(s) 390, 545, 852, 1190, 1520
BciVI GTATCC 1 cut(s) 551
BclI TGATCA 1 cut(s) 1591
BcoDI GTCTC 2 cut(s) 1164, 1561
BcuI ACTAGT 1 cut(s) 536
BfaI CTAG 4 cut(s) 537, 569, 804, 1499
BfmI CTRYAG 1 cut(s) 940
BfuAI ACCTGC 1 cut(s) 937
BfuI GTATCC 1 cut(s) 551
BglI GCCNNNNNGGC 1 cut(s) 60
BglII AGATCT 1 cut(s) 1281
BisI GCNGC 4 cut(s) 64, 233, 933, 1092
BlpI GCTNAGC 1 cut(s) 637
BlsI GCNGC 4 cut(s) 65, 234, 934, 1093
BmcAI AGTACT 2 cut(s) 402, 1368
Bme1390I CCNGG 5 cut(s) 390, 545, 852, 1190, 1520
Bme18I GGWCC 3 cut(s) 195, 744, 1400
BmeT110I CYCGRG 1 cut(s) 120
BmgBI CACGTC 1 cut(s) 614
BmgT120I GGNCC 3 cut(s) 195, 744, 1400
BmiI GGNNCC 3 cut(s) 242, 927, 1401
BmrFI CCNGG 5 cut(s) 390, 545, 852, 1190, 1520
BmsI GCATC 3 cut(s) 555, 742, 1206
BpiI GAAGAC 1 cut(s) 411
BplI GAGNNNNNCTC 2 cut(s) 474, 506
BpmI CTGGAG 1 cut(s) 834
Bpu1102I GCTNAGC 1 cut(s) 637
BpuEI CTTGAG 1 cut(s) 1034
BsaBI GATNNNNATC 2 cut(s) 519, 675
BsaJI CCNNGG 7 cut(s) 73, 198, 388, 544, 892, 1102, 1451
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bsc4I CCNNNNNNNGG 6 cut(s) 226, 365, 809, 1189, 1457, 1504
Bse1I ACTGG 2 cut(s) 312, 1364
Bse3DI GCAATG 3 cut(s) 242, 376, 999
Bse8I GATNNNNATC 2 cut(s) 519, 675
BseBI CCWGG 5 cut(s) 390, 545, 852, 1190, 1520
BseDI CCNNGG 7 cut(s) 73, 198, 388, 544, 892, 1102, 1451
BseGI GGATG 1 cut(s) 860
BseJI GATNNNNATC 2 cut(s) 519, 675
BseLI CCNNNNNNNGG 6 cut(s) 226, 365, 809, 1189, 1457, 1504
BseMI GCAATG 3 cut(s) 242, 376, 999
BseMII CTCAG 4 cut(s) 785, 855, 1146, 1310
BseNI ACTGG 2 cut(s) 312, 1364
BseRI GAGGAG 2 cut(s) 129, 1046
BseXI GCAGC 4 cut(s) 75, 219, 919, 1078
BseYI CCCAGC 2 cut(s) 1289, 1380
BshFI GGCC 1 cut(s) 802
BshNI GGYRCC 2 cut(s) 240, 925
BsiHKAI GWGCWC 1 cut(s) 121
BsiHKCI CYCGRG 1 cut(s) 120
BsiSI CCGG 1 cut(s) 1544
BslFI GGGAC 1 cut(s) 1473
BslI CCNNNNNNNGG 6 cut(s) 226, 365, 809, 1189, 1457, 1504
BsmAI GTCTC 2 cut(s) 1164, 1561
BsmFI GGGAC 1 cut(s) 1473
BsmI GAATGC 1 cut(s) 268
BsnI GGCC 1 cut(s) 802
BsoBI CYCGRG 1 cut(s) 120
Bsp1286I GDGCHC 1 cut(s) 121
Bsp143I GATC 5 cut(s) 607, 967, 1281, 1420, 1591
Bsp1720I GCTNAGC 1 cut(s) 637
Bsp19I CCATGG 1 cut(s) 73
BspACI CCGC 2 cut(s) 742, 755
BspANI GGCC 1 cut(s) 802
BspCNI CTCAG 4 cut(s) 786, 856, 1147, 1311
BspLI GGNNCC 3 cut(s) 242, 927, 1401
BspMI ACCTGC 1 cut(s) 937
BspPI GGATC 1 cut(s) 615
BspT107I GGYRCC 2 cut(s) 240, 925
BsrDI GCAATG 3 cut(s) 242, 376, 999
BsrI ACTGG 2 cut(s) 312, 1364
BssECI CCNNGG 7 cut(s) 73, 198, 388, 544, 892, 1102, 1451
BssMI GATC 5 cut(s) 607, 967, 1281, 1420, 1591
BssSI CACGAG 1 cut(s) 579
BssT1I CCWWGG 3 cut(s) 73, 198, 1102
Bst2BI CACGAG 1 cut(s) 579
Bst2UI CCWGG 5 cut(s) 390, 545, 852, 1190, 1520
Bst4CI ACNGT 6 cut(s) 89, 718, 944, 1074, 1140, 1258
Bst6I CTCTTC 1 cut(s) 1530
BstAPI GCANNNNNTGC 1 cut(s) 932
BstDEI CTNAG 8 cut(s) 93, 550, 637, 659, 794, 864, 1155, 1319
BstDSI CCRYGG 2 cut(s) 73, 892
BstENI CCTNNNNNAGG 1 cut(s) 807
BstF5I GGATG 1 cut(s) 860
BstKTI GATC 5 cut(s) 610, 970, 1284, 1423, 1594
BstMAI GTCTC 2 cut(s) 1164, 1561
BstMBI GATC 5 cut(s) 607, 967, 1281, 1420, 1591
BstMWI GCNNNNNNNGC 4 cut(s) 60, 125, 238, 932
BstNI CCWGG 5 cut(s) 390, 545, 852, 1190, 1520
BstNSI RCATGY 2 cut(s) 686, 1349
BstSCI CCNGG 5 cut(s) 388, 543, 850, 1188, 1518
BstSFI CTRYAG 1 cut(s) 940
BstV1I GCAGC 4 cut(s) 75, 219, 919, 1078
BstV2I GAAGAC 1 cut(s) 411
BstX2I RGATCY 1 cut(s) 1281
BstXI CCANNNNNNTGG 2 cut(s) 581, 858
BstYI RGATCY 1 cut(s) 1281
BsuI GTATCC 1 cut(s) 551
BsuRI GGCC 1 cut(s) 802
BtgI CCRYGG 2 cut(s) 73, 892
BtrI CACGTC 1 cut(s) 614
BtsCI GGATG 1 cut(s) 860
BtsIMutI CAGTG 1 cut(s) 307
BveI ACCTGC 1 cut(s) 937
Cfr13I GGNCC 3 cut(s) 195, 744, 1400
Csp6I GTAC 7 cut(s) 90, 305, 401, 534, 1136, 1259, 1367
CspCI CAANNNNNGTGG 2 cut(s) 874, 909
CviAII CATG 8 cut(s) 74, 344, 423, 493, 683, 1096, 1346, 1595
CviQI GTAC 7 cut(s) 90, 305, 401, 534, 1136, 1259, 1367
DdeI CTNAG 8 cut(s) 93, 550, 637, 659, 794, 864, 1155, 1319
DpnI GATC 5 cut(s) 609, 969, 1283, 1422, 1593
DpnII GATC 5 cut(s) 607, 967, 1281, 1420, 1591
DraI TTTAAA 2 cut(s) 592, 1147
Eam1104I CTCTTC 1 cut(s) 1530
EarI CTCTTC 1 cut(s) 1530
Ecl136II GAGCTC 1 cut(s) 119
Eco130I CCWWGG 3 cut(s) 73, 198, 1102
Eco24I GRGCYC 1 cut(s) 121
Eco47I GGWCC 3 cut(s) 195, 744, 1400
Eco53kI GAGCTC 1 cut(s) 119
Eco57I CTGAAG 1 cut(s) 389
Eco88I CYCGRG 1 cut(s) 120
EcoICRI GAGCTC 1 cut(s) 119
EcoNI CCTNNNNNAGG 1 cut(s) 807
EcoO109I RGGNCCY 1 cut(s) 1400
EcoRII CCWGG 5 cut(s) 388, 543, 850, 1188, 1518
EcoT14I CCWWGG 3 cut(s) 73, 198, 1102
EcoT22I ATGCAT 1 cut(s) 1351
EcoT38I GRGCYC 1 cut(s) 121
ErhI CCWWGG 3 cut(s) 73, 198, 1102
FaeI CATG 8 cut(s) 77, 347, 426, 496, 686, 1099, 1349, 1598
FaqI GGGAC 1 cut(s) 1473
FatI CATG 8 cut(s) 73, 343, 422, 492, 682, 1095, 1345, 1594
FauNDI CATATG 1 cut(s) 156
FbaI TGATCA 1 cut(s) 1591
Fnu4HI GCNGC 4 cut(s) 64, 233, 933, 1092
FokI GGATG 1 cut(s) 867
FriOI GRGCYC 1 cut(s) 121
Fsp4HI GCNGC 4 cut(s) 64, 233, 933, 1092
FspBI CTAG 4 cut(s) 537, 569, 804, 1499
GluI GCNGC 4 cut(s) 64, 233, 933, 1092
GsaI CCCAGC 2 cut(s) 1293, 1384
GsuI CTGGAG 1 cut(s) 834
HaeIII GGCC 1 cut(s) 802
HapII CCGG 1 cut(s) 1544
Hin1II CATG 8 cut(s) 77, 347, 426, 496, 686, 1099, 1349, 1598
HincII GTYRAC 1 cut(s) 18
HindII GTYRAC 1 cut(s) 18
HindIII AAGCTT 2 cut(s) 586, 1337
HpaII CCGG 1 cut(s) 1544
HphI GGTGA 1 cut(s) 1118
Hpy166II GTNNAC 4 cut(s) 18, 305, 341, 488
Hpy188I TCNGA 7 cut(s) 316, 322, 795, 865, 1156, 1320, 1455
Hpy188III TCNNGA 4 cut(s) 1013, 1201, 1304, 1424
Hpy8I GTNNAC 4 cut(s) 18, 305, 341, 488
Hpy99I CGWCG 1 cut(s) 615
HpyAV CCTTC 3 cut(s) 916, 1450, 1457
HpyCH4III ACNGT 6 cut(s) 89, 718, 944, 1074, 1140, 1258
HpyCH4IV ACGT 2 cut(s) 613, 1480
HpyF10VI GCNNNNNNNGC 4 cut(s) 60, 125, 238, 932
HpyF3I CTNAG 8 cut(s) 93, 550, 637, 659, 794, 864, 1155, 1319
HpySE526I ACGT 2 cut(s) 613, 1480
Hsp92II CATG 8 cut(s) 77, 347, 426, 496, 686, 1099, 1349, 1598
Ksp22I TGATCA 1 cut(s) 1591
Kzo9I GATC 5 cut(s) 607, 967, 1281, 1420, 1591
LmnI GCTCC 3 cut(s) 116, 229, 1546
Lsp1109I GCAGC 4 cut(s) 75, 219, 919, 1078
LweI GCATC 3 cut(s) 555, 742, 1206
MaeI CTAG 4 cut(s) 537, 569, 804, 1499
MaeII ACGT 2 cut(s) 613, 1480
MaeIII GTNAC 1 cut(s) 1615
MalI GATC 5 cut(s) 609, 969, 1283, 1422, 1593
MboI GATC 5 cut(s) 607, 967, 1281, 1420, 1591
MfeI CAATTG 2 cut(s) 375, 957
MflI RGATCY 1 cut(s) 1281
MhlI GDGCHC 1 cut(s) 121
MlyI GAGTC 4 cut(s) 287, 805, 841, 1138
MnlI CCTC 9 cut(s) 107, 476, 598, 732, 886, 1059, 1067, 1114, 1461
Mph1103I ATGCAT 1 cut(s) 1351
MseI TTAA 7 cut(s) 36, 591, 647, 735, 1146, 1299, 1511
MspA1I CMGCKG 1 cut(s) 1091
MspI CCGG 1 cut(s) 1544
MspR9I CCNGG 5 cut(s) 390, 545, 852, 1190, 1520
MunI CAATTG 2 cut(s) 375, 957
Mva1269I GAATGC 1 cut(s) 268
MvaI CCWGG 5 cut(s) 390, 545, 852, 1190, 1520
MwoI GCNNNNNNNGC 4 cut(s) 60, 125, 238, 932
NcoI CCATGG 1 cut(s) 73
NdeI CATATG 1 cut(s) 156
NdeII GATC 5 cut(s) 607, 967, 1281, 1420, 1591
NlaIII CATG 8 cut(s) 77, 347, 426, 496, 686, 1099, 1349, 1598
NlaIV GGNNCC 3 cut(s) 242, 927, 1401
NmuCI GTSAC 1 cut(s) 1615
NsiI ATGCAT 1 cut(s) 1351
NspI RCATGY 2 cut(s) 686, 1349
PaeR7I CTCGAG 1 cut(s) 120
PaqCI CACCTGC 1 cut(s) 937
PcsI WCGNNNNNNNCGW 1 cut(s) 529
PctI GAATGC 1 cut(s) 268
PflFI GACNNNGTC 1 cut(s) 296
PfoI TCCNGGA 2 cut(s) 850, 1188
PkrI GCNGC 4 cut(s) 65, 234, 934, 1093
PleI GAGTC 4 cut(s) 287, 804, 841, 1137
PpsI GAGTC 4 cut(s) 287, 804, 841, 1137
PpuMI RGGWCCY 1 cut(s) 1400
PshBI ATTAAT 1 cut(s) 647
Psp124BI GAGCTC 1 cut(s) 121
Psp5II RGGWCCY 1 cut(s) 1400
Psp6I CCWGG 5 cut(s) 388, 543, 850, 1188, 1518
PspFI CCCAGC 2 cut(s) 1289, 1380
PspGI CCWGG 5 cut(s) 388, 543, 850, 1188, 1518
PspN4I GGNNCC 3 cut(s) 242, 927, 1401
PspPI GGNCC 3 cut(s) 195, 744, 1400
PspPPI RGGWCCY 1 cut(s) 1400
PspXI VCTCGAGB 1 cut(s) 120
PsuI RGATCY 1 cut(s) 1281
PsyI GACNNNGTC 1 cut(s) 296
PvuII CAGCTG 1 cut(s) 1091
RsaI GTAC 7 cut(s) 91, 306, 402, 535, 1137, 1260, 1368
RsaNI GTAC 7 cut(s) 90, 305, 401, 534, 1136, 1259, 1367
SacI GAGCTC 1 cut(s) 121
SaqAI TTAA 7 cut(s) 36, 591, 647, 735, 1146, 1299, 1511
SatI GCNGC 4 cut(s) 64, 233, 933, 1092
Sau3AI GATC 5 cut(s) 607, 967, 1281, 1420, 1591
Sau96I GGNCC 3 cut(s) 195, 744, 1400
ScaI AGTACT 2 cut(s) 402, 1368
SchI GAGTC 4 cut(s) 287, 805, 841, 1138
ScrFI CCNGG 5 cut(s) 390, 545, 852, 1190, 1520
SduI GDGCHC 1 cut(s) 121
SfaNI GCATC 3 cut(s) 555, 742, 1206
SfcI CTRYAG 1 cut(s) 940
Sfr274I CTCGAG 1 cut(s) 120
SinI GGWCC 3 cut(s) 195, 744, 1400
SlaI CTCGAG 1 cut(s) 120
SmlI CTYRAG 2 cut(s) 120, 1013
SmoI CTYRAG 2 cut(s) 120, 1013
SpeI ACTAGT 1 cut(s) 536
SsiI CCGC 2 cut(s) 742, 755
SspMI CTAG 4 cut(s) 537, 569, 804, 1499
SstI GAGCTC 1 cut(s) 121
StyD4I CCNGG 5 cut(s) 388, 543, 850, 1188, 1518
StyI CCWWGG 3 cut(s) 73, 198, 1102
TaaI ACNGT 6 cut(s) 89, 718, 944, 1074, 1140, 1258
TaiI ACGT 2 cut(s) 616, 1483
TaqI TCGA 4 cut(s) 121, 610, 643, 1253
TatI WGTACW 4 cut(s) 304, 400, 1135, 1366
Tru1I TTAA 7 cut(s) 36, 591, 647, 735, 1146, 1299, 1511
Tru9I TTAA 7 cut(s) 36, 591, 647, 735, 1146, 1299, 1511
TscAI CASTG 1 cut(s) 307
TseFI GTSAC 1 cut(s) 1615
TseI GCWGC 4 cut(s) 63, 232, 932, 1091
Tsp45I GTSAC 1 cut(s) 1615
TspDTI ATGAA 9 cut(s) 294, 411, 426, 465, 519, 783, 1233, 1323, 1421
TspGWI ACGGA 4 cut(s) 242, 286, 617, 881
TspRI CASTG 1 cut(s) 307
Tth111I GACNNNGTC 1 cut(s) 296
VpaK11BI GGWCC 3 cut(s) 195, 744, 1400
VspI ATTAAT 1 cut(s) 647
XagI CCTNNNNNAGG 1 cut(s) 807
XapI RAATTY 4 cut(s) 68, 215, 1159, 1388
XceI RCATGY 2 cut(s) 686, 1349
XhoI CTCGAG 1 cut(s) 120
XspI CTAG 4 cut(s) 537, 569, 804, 1499
ZrmI AGTACT 2 cut(s) 402, 1368
Zsp2I ATGCAT 1 cut(s) 1351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.