AT5G39430

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005242
Physical Location & Seq
Reverse (-)
15775302 .. 15777599
2298 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G39430.1

Sequence Viewer

Length: 1536 bp
ATGGGTACATGTATGTCAACACATTCAAGAAGACTTAGGCCACGAAGGAAAGGCCGTCGCAGATTTTCTAAAAACATATCTAAAGTGTCTGATATTAGACGCCTAAGTGATGTTGGAATCCAAACCTCTTTTGATATTTCTCAAAACGATGCATGGTTTGATTCTTCAAGTCTTTTCTCTGACTCTGATGACGACTTTATTAGCCTACATGAAGCTGATAATGTTTGGTTAGAGGGTGGCGTAATGGGTAAAATTCCAAATGGTCAAGTGGTTGAGTTTGAGGCTTCTTCATGCATTGTTGATGGAAATGGAAATTACGAAGAATACCATGAGAGTTACTTGAAGATTGATGGCGGTAATAAAATCGAGAAGTTTATGAGCAACGGTTTATATAAAGATACTAATGGTTTGTCTGGTATTATTGGAAACAACAAGAAGAAACTAAATACTTATAGTAGCTTTAAAGGATTGAAAGAGTTAGACCCTAACCCTAAAGAAAAAGCATTAAAATCCAATTTGAGCCGGTTGATGCCATTGCCAACGGTTAGTTTCAACGACAAAACTCTGAACTCCCCCACATCTCAAAACCGGAAGTCAGCGGTTTATCAGGTTTCTTTCAAGAGAAGATCATGTGATGGTGAAGAAGTTACCGAACATAGGTCGTCGAAAAGATTGTTGTACCGTCCCAAAGCCGGATACACGATTCCGTGCTATGTCAAAGAGAAGCATCAATCATCAGGAAGTTGGTGTGAGATACCTCCATCAAACTTGAAGTTACGTGGCGAAACTTATTTCAAAGATAAACGAAAACACCCGGCTCCAAACCAATGTCCATACACTCCAATTGGTGTTGACTTGTTTGTGTGTCCAAGGAAGATTGATCATATAGCCCAACACATTGAGCTTCCCAACATCAAAGCTGTGGCAAACCTCCCTGCTCTTCTAATTGTCAACATCCAGTTGCCAACTTATCCAGCCGCAATGTTTCTCGGTGATAGTAACGGAGAAGGCATGAGCATTGTACTTTACTTTAAACTACGAGAAAATTTCAAAAATGAAATCTCTCAACAATACCAAGACAGCATCAAGAAACTAGTGGAAGATGAGATGGAAAAAGTTAAAGGGTTTGCAAAAGACAACATCGTTCCGTTTCGTGAAAGGCTTAAAATAGTAGCCGGATTAGTTAACCCAGATGAACTCAGTCTGAGTTCTACTGAAAAGAAGCTTATACAAGCTTACAATGAAAAACCCGTGCTTTCTCGCCCTCAACACAACTTCTTCAAGGGTCCTAACTACTTTGAGATTGATTTGGACGTGCATCGGTTTAGCTACCTTTCAAGAAAAGGGCTTGAAGCATTCAGAGATCGACTCAAAAATGGAACTCTTGATCTCGGCTTAACTATCCAAGCTCAAAAACAAGAGGAGTTACCAGAAAAGGTCCTATGTTGTCTAAGATTGAGCAAGATTGACTTTGTTGACAACGGTCAAATTCCTACACTTTTAATTCCTGAAGAAGGAGAATCATTCCCTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

511

Amino Acids

58.06

Weight (kDa)

8.92

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 249 - 490 9e-61 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 354, 599, 978
AcsI RAATTY 3 cut(s) 252, 1045, 1488
AcuI CTGAAG 1 cut(s) 1530
AcyI GRCGYC 1 cut(s) 100
AfaI GTAC 3 cut(s) 7, 680, 1023
AfiI CCNNNNNNNGG 3 cut(s) 657, 692, 1514
AflIII ACRYGT 1 cut(s) 8
AhlI ACTAGT 1 cut(s) 1093
AjiI CACGTC 1 cut(s) 1315
AluBI AGCT 8 cut(s) 215, 459, 904, 920, 1225, 1235, 1329, 1409
AluI AGCT 8 cut(s) 215, 459, 904, 920, 1225, 1235, 1329, 1409
AoxI GGCC 2 cut(s) 38, 52
ApoI RAATTY 3 cut(s) 252, 1045, 1488
AspS9I GGNCC 2 cut(s) 1286, 1438
AsuC2I CCSGG 1 cut(s) 815
AsuHPI GGTGA 2 cut(s) 650, 1004
AvaII GGWCC 2 cut(s) 1286, 1438
BbsI GAAGAC 1 cut(s) 37
BccI CCATC 5 cut(s) 296, 344, 629, 769, 1102
BceAI ACGGC 1 cut(s) 39
BciVI GTATCC 1 cut(s) 689
BclI TGATCA 1 cut(s) 880
BcnI CCSGG 1 cut(s) 815
BcuI ACTAGT 1 cut(s) 1093
BfaI CTAG 1 cut(s) 1094
BfuI GTATCC 1 cut(s) 689
BisI GCNGC 1 cut(s) 978
BlsI GCNGC 1 cut(s) 979
Bme1390I CCNGG 1 cut(s) 815
Bme18I GGWCC 2 cut(s) 1286, 1438
BmgBI CACGTC 1 cut(s) 1315
BmgT120I GGNCC 2 cut(s) 1286, 1438
BmiI GGNNCC 2 cut(s) 819, 1287
BmrFI CCNGG 1 cut(s) 815
BmsI GCATC 5 cut(s) 139, 519, 736, 1092, 1327
BoxI GACNNNNGTC 1 cut(s) 1482
BpiI GAAGAC 1 cut(s) 37
BplI GAGNNNNNCTC 2 cut(s) 1353, 1385
BpuMI CCSGG 1 cut(s) 815
BsaAI YACGTR 1 cut(s) 779
BsaHI GRCGYC 1 cut(s) 100
BsaJI CCNNGG 1 cut(s) 869
BsaWI WCCGGW 1 cut(s) 588
Bsc4I CCNNNNNNNGG 3 cut(s) 657, 692, 1514
Bse118I RCCGGY 1 cut(s) 522
Bse1I ACTGG 1 cut(s) 958
Bse3DI GCAATG 2 cut(s) 533, 987
BseDI CCNNGG 1 cut(s) 869
BseGI GGATG 1 cut(s) 954
BseLI CCNNNNNNNGG 3 cut(s) 657, 692, 1514
BseMI GCAATG 2 cut(s) 533, 987
BseMII CTCAG 2 cut(s) 1196, 1213
BseNI ACTGG 1 cut(s) 958
BseRI GAGGAG 1 cut(s) 1436
BshFI GGCC 2 cut(s) 40, 54
BsiSI CCGG 5 cut(s) 523, 589, 693, 815, 1176
BslFI GGGAC 1 cut(s) 669
BslI CCNNNNNNNGG 3 cut(s) 657, 692, 1514
BsmFI GGGAC 1 cut(s) 669
BsmI GAATGC 1 cut(s) 1355
BsnI GGCC 2 cut(s) 40, 54
Bsp143I GATC 4 cut(s) 626, 880, 1363, 1387
BspACI CCGC 3 cut(s) 354, 599, 978
BspANI GGCC 2 cut(s) 40, 54
BspCNI CTCAG 2 cut(s) 1197, 1212
BspLI GGNNCC 2 cut(s) 819, 1287
BspQI GCTCTTC 1 cut(s) 945
BsrDI GCAATG 2 cut(s) 533, 987
BsrFI RCCGGY 1 cut(s) 522
BsrI ACTGG 1 cut(s) 958
BssAI RCCGGY 1 cut(s) 522
BssECI CCNNGG 1 cut(s) 869
BssMI GATC 4 cut(s) 626, 880, 1363, 1387
BssNI GRCGYC 1 cut(s) 100
BssT1I CCWWGG 1 cut(s) 869
Bst4CI ACNGT 4 cut(s) 386, 544, 683, 1484
Bst6I CTCTTC 1 cut(s) 945
BstACI GRCGYC 1 cut(s) 100
BstBAI YACGTR 1 cut(s) 779
BstDEI CTNAG 5 cut(s) 35, 104, 1199, 1205, 1451
BstENI CCTNNNNNAGG 1 cut(s) 1512
BstF5I GGATG 1 cut(s) 954
BstKTI GATC 4 cut(s) 629, 883, 1366, 1390
BstMBI GATC 4 cut(s) 626, 880, 1363, 1387
BstNSI RCATGY 1 cut(s) 12
BstPAI GACNNNNGTC 1 cut(s) 1482
BstSCI CCNGG 1 cut(s) 813
BstV2I GAAGAC 1 cut(s) 37
BsuI GTATCC 1 cut(s) 689
BsuRI GGCC 2 cut(s) 40, 54
BtrI CACGTC 1 cut(s) 1315
BtsCI GGATG 1 cut(s) 954
Cfr10I RCCGGY 1 cut(s) 522
Cfr13I GGNCC 2 cut(s) 1286, 1438
CseI GACGC 1 cut(s) 108
Csp6I GTAC 3 cut(s) 6, 679, 1022
CviAII CATG 7 cut(s) 9, 153, 209, 291, 329, 630, 1012
CviQI GTAC 3 cut(s) 6, 679, 1022
DdeI CTNAG 5 cut(s) 35, 104, 1199, 1205, 1451
DpnI GATC 4 cut(s) 628, 882, 1365, 1389
DpnII GATC 4 cut(s) 626, 880, 1363, 1387
DraI TTTAAA 2 cut(s) 463, 1033
Eam1104I CTCTTC 1 cut(s) 945
EarI CTCTTC 1 cut(s) 945
Eco130I CCWWGG 1 cut(s) 869
Eco47I GGWCC 2 cut(s) 1286, 1438
Eco57I CTGAAG 1 cut(s) 1530
EcoNI CCTNNNNNAGG 1 cut(s) 1512
EcoO109I RGGNCCY 2 cut(s) 1286, 1438
EcoT14I CCWWGG 1 cut(s) 869
EcoT22I ATGCAT 2 cut(s) 154, 296
ErhI CCWWGG 1 cut(s) 869
FaeI CATG 7 cut(s) 12, 156, 212, 294, 332, 633, 1015
FalI AAGNNNNNCTT 2 cut(s) 1454, 1486
FaqI GGGAC 1 cut(s) 669
FatI CATG 7 cut(s) 8, 152, 208, 290, 328, 629, 1011
FbaI TGATCA 1 cut(s) 880
Fnu4HI GCNGC 1 cut(s) 978
FokI GGATG 1 cut(s) 941
Fsp4HI GCNGC 1 cut(s) 978
FspBI CTAG 1 cut(s) 1094
GluI GCNGC 1 cut(s) 978
HaeIII GGCC 2 cut(s) 40, 54
HapII CCGG 5 cut(s) 523, 589, 693, 815, 1176
HgaI GACGC 1 cut(s) 108
Hin1I GRCGYC 1 cut(s) 100
Hin1II CATG 7 cut(s) 12, 156, 212, 294, 332, 633, 1015
HincII GTYRAC 5 cut(s) 18, 853, 952, 1186, 1477
HindII GTYRAC 5 cut(s) 18, 853, 952, 1186, 1477
HindIII AAGCTT 2 cut(s) 1223, 1233
HinfI GANTC 6 cut(s) 117, 161, 182, 703, 1368, 1520
HpaI GTTAAC 1 cut(s) 1186
HpaII CCGG 5 cut(s) 523, 589, 693, 815, 1176
HphI GGTGA 2 cut(s) 650, 1004
Hpy166II GTNNAC 5 cut(s) 18, 853, 952, 1186, 1477
Hpy188I TCNGA 6 cut(s) 91, 181, 187, 567, 1206, 1361
Hpy188III TCNNGA 9 cut(s) 27, 367, 619, 738, 1087, 1154, 1338, 1385, 1508
Hpy8I GTNNAC 5 cut(s) 18, 853, 952, 1186, 1477
Hpy99I CGWCG 2 cut(s) 60, 667
HpyAV CCTTC 3 cut(s) 39, 1001, 1508
HpyCH4III ACNGT 4 cut(s) 386, 544, 683, 1484
HpyCH4IV ACGT 2 cut(s) 778, 1314
HpyCH4V TGCA 4 cut(s) 152, 294, 1130, 1318
HpyF3I CTNAG 5 cut(s) 35, 104, 1199, 1205, 1451
HpySE526I ACGT 2 cut(s) 778, 1314
Hsp92I GRCGYC 1 cut(s) 100
Hsp92II CATG 7 cut(s) 12, 156, 212, 294, 332, 633, 1015
Ksp22I TGATCA 1 cut(s) 880
KspAI GTTAAC 1 cut(s) 1186
Kzo9I GATC 4 cut(s) 626, 880, 1363, 1387
LguI GCTCTTC 1 cut(s) 945
LmnI GCTCC 1 cut(s) 823
LweI GCATC 5 cut(s) 139, 519, 736, 1092, 1327
MaeI CTAG 1 cut(s) 1094
MaeII ACGT 2 cut(s) 778, 1314
MaeIII GTNAC 5 cut(s) 335, 646, 774, 998, 1425
MalI GATC 4 cut(s) 628, 882, 1365, 1389
MboI GATC 4 cut(s) 626, 880, 1363, 1387
MfeI CAATTG 1 cut(s) 843
MluCI AATT 8 cut(s) 252, 313, 514, 843, 945, 1045, 1488, 1503
MlyI GAGTC 2 cut(s) 176, 1362
MmeI TCCRAC 1 cut(s) 94
MnlI CCTC 7 cut(s) 136, 226, 274, 768, 941, 1275, 1414
Mph1103I ATGCAT 2 cut(s) 154, 296
MseI TTAA 8 cut(s) 462, 506, 1032, 1119, 1164, 1185, 1397, 1502
MspA1I CMGCKG 1 cut(s) 599
MspI CCGG 5 cut(s) 523, 589, 693, 815, 1176
MspR9I CCNGG 1 cut(s) 815
MunI CAATTG 1 cut(s) 843
Mva1269I GAATGC 1 cut(s) 1355
NciI CCSGG 1 cut(s) 815
NdeII GATC 4 cut(s) 626, 880, 1363, 1387
NlaIII CATG 7 cut(s) 12, 156, 212, 294, 332, 633, 1015
NlaIV GGNNCC 2 cut(s) 819, 1287
NmeAIII GCCGAG 1 cut(s) 1371
NsiI ATGCAT 2 cut(s) 154, 296
NspI RCATGY 1 cut(s) 12
PciI ACATGT 1 cut(s) 8
PciSI GCTCTTC 1 cut(s) 945
PctI GAATGC 1 cut(s) 1355
PfeI GAWTC 4 cut(s) 117, 161, 703, 1520
PkrI GCNGC 1 cut(s) 979
PleI GAGTC 2 cut(s) 176, 1362
PpsI GAGTC 2 cut(s) 176, 1362
Ppu21I YACGTR 1 cut(s) 779
PpuMI RGGWCCY 2 cut(s) 1286, 1438
PscI ACATGT 1 cut(s) 8
PshAI GACNNNNGTC 1 cut(s) 1482
Psp5II RGGWCCY 2 cut(s) 1286, 1438
PspN4I GGNNCC 2 cut(s) 819, 1287
PspPI GGNCC 2 cut(s) 1286, 1438
PspPPI RGGWCCY 2 cut(s) 1286, 1438
RsaI GTAC 3 cut(s) 7, 680, 1023
RsaNI GTAC 3 cut(s) 6, 679, 1022
SapI GCTCTTC 1 cut(s) 945
SaqAI TTAA 8 cut(s) 462, 506, 1032, 1119, 1164, 1185, 1397, 1502
SatI GCNGC 1 cut(s) 978
Sau3AI GATC 4 cut(s) 626, 880, 1363, 1387
Sau96I GGNCC 2 cut(s) 1286, 1438
SchI GAGTC 2 cut(s) 176, 1362
ScrFI CCNGG 1 cut(s) 815
SfaNI GCATC 5 cut(s) 139, 519, 736, 1092, 1327
SinI GGWCC 2 cut(s) 1286, 1438
SpeI ACTAGT 1 cut(s) 1093
Sse9I AATT 8 cut(s) 252, 313, 514, 843, 945, 1045, 1488, 1503
SsiI CCGC 3 cut(s) 354, 599, 978
SspMI CTAG 1 cut(s) 1094
StyD4I CCNGG 1 cut(s) 813
StyI CCWWGG 1 cut(s) 869
TaaI ACNGT 4 cut(s) 386, 544, 683, 1484
TaiI ACGT 2 cut(s) 781, 1317
TaqI TCGA 3 cut(s) 366, 665, 1366
TasI AATT 8 cut(s) 252, 313, 514, 843, 945, 1045, 1488, 1503
TatI WGTACW 1 cut(s) 1021
TauI GCSGC 1 cut(s) 980
TfiI GAWTC 4 cut(s) 117, 161, 703, 1520
Tru1I TTAA 8 cut(s) 462, 506, 1032, 1119, 1164, 1185, 1397, 1502
Tru9I TTAA 8 cut(s) 462, 506, 1032, 1119, 1164, 1185, 1397, 1502
TspDTI ATGAA 5 cut(s) 225, 279, 1071, 1209, 1257
TspGWI ACGGA 3 cut(s) 696, 1017, 1137
VpaK11BI GGWCC 2 cut(s) 1286, 1438
XagI CCTNNNNNAGG 1 cut(s) 1512
XapI RAATTY 3 cut(s) 252, 1045, 1488
XceI RCATGY 1 cut(s) 12
XspI CTAG 1 cut(s) 1094
Zsp2I ATGCAT 2 cut(s) 154, 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.