AT3G29400

exocyst complex component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009068
Physical Location & Seq
Reverse (-)
11297180 .. 11299695
2516 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G29400.1

Sequence Viewer

Length: 1977 bp
ATGGGAGAGTTTGAGGTAGATGGAGAAGAGAAACTGATTGTTGCTGCTAAGTATCTTGTTAAGGAACTTAGATCTGGTAAGAGTCTTACTAAAAATGCTAAGAATGTTTTAGGCAATCTCTTATTAGAGCTTTCTCGTGTGGTGATAGCTGAGGATACTCAGGATAGAGATGAGGAAGATGAAATAGGTGAGATTGAGGAAAGGCTTAATGTTGTTAGTGAGAAGATTATGACTAGAGAAGTCGATGAATCAATGATTTGGGATTTAGGTTCAGATGAAGGTAATCTGTATTTGGATGCTGTGAATGAGTTGAGAAGTTTGATTGATCGGTTAGACGGAAGTGAGGAGCTTTCTTTAAGAAAAGCTCATGATGTTCTTCAAATAGCTATGGCACGGCTTGAGGATGAGTTTAAGCATCTTCTTGTGGAGAATAGATTGCCTTTTGAGCTTGAACATTCGTCGTTTCGGTCTATTGAAGCGGATCATGGTGTGGAGGAAGAGTCCATGGCTTCTTTTGGTGCTGCTTCTACTGAGGATTTGATTCTTGGAAGTAATAATGATAGTAGGAGAAACTCAGGGGACGTTGTGGTTGATTTGGTGAATCCTGATGTTATATTAGATCTCAAGAACATTGCGAATACTATGATTGCTTCAGGGTATGACCGTGAGTGTATTCAGGTGTGTACAATGGTTAGAAAAGATGCTTTGGATGAGTTTTTGTATAACCATGAGGTTGAGAAGTTGAGTATTGAAGATGTTTTGAGGATGGATTGGGCGACGTTGAATACGAATATCAAGAAATGGGTTCGGGTTATGAGAGATATCGTCCAGGTATATCTCTTGAGTGAGAAGTCTCTGGATAATCAGATCTTTGGAGACTTGAATGAGATCGGTCTAACATGTTTCGTCGATACTGTGAAGGCTCCGATGATGCAGTTGCTTAATTTTGGTGAAGCTGTGTCTCTTGGTCCACGACAACCTGAGAAATTGCTTAGGATTCTTGAAATGTATGAGCTAGCATCTGAGCTTTTACCAGAGATCGATGCTTTGTTCTTGGATCATCCCGGTTCGTCCGTAAGAACCGAATACAGGGAAGTGATGAGGAGACTTGGGGATTGTGCAAGAACAACGTTTCTTGAATTCAAAAGTGCGATTGCAGCGGATGTCTCATCGCATCCTTTCCCTGGAGGCGCGGTTCACCCGCTTACAAACTACGTGATGAATTACCTCATGGCTCTAACAGATTTCAAACACACGCTCGACTCTCTTCTAATGGAACATGATGATGCAGAAGATCTCACAATACCACCATCGCCGGATATTATCAATCCCGTGATGGTCGAAGAAGAGTCTACCTACGAAAACTCCTCTTCACCGGAGAAATTTCTGGCAATGACCCGACATTTCTACTCCATAACCTCTGTCCTCGAAGCCAACCTTCAAGAGAAATCAAAACTATACAAAGATGTCTCTCTACAACACATTTTTCTCCTCAACAACATACACTACATGACCCGGAAAGTGCTAAAGTCCGAGCTGAGACACATCTTCGGCGACAAATGGAACCGAAAACATACATGGAAGTTTCAGCAGCAAGCAACAGAGTACGAACGCGCAACCTGGCTCCCTGTCCTATCGTTCCTCAAGGACGATGGTTCAGGTTCTGGTCCCGGTTCTGGCTCAGGTTCTGGTTCAAAGAATCTACGCCCGAGGGAGCGGTTCCAAGGATTCAACACTGCGTTCGAGGAAGTGTACAAAGCGCAAACCGGATGGCTAATTTCGGACGAGGGGCTAAGAGAAGATGTAAGGACAAAGGCGTCCATGTGGGTGATTCAAGCGTATTGGACATTTTACAGTAGACACAAGAACAGTGTCAGTGAAAGGTATATAAAGTACACTACTGATGATATTGAGAGACTCTTGTTGGATCTCTTTGCTGGTTCTTCTAAATCCTTGAACAATTCTTATAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0006887 GO:0008150 GO:0009987 GO:0016192 GO:0032940 GO:0046903 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
ko04910 map04910
Pfam Domains
Protein Families
Exo70

Protein Analysis

658

Amino Acids

75.23

Weight (kDa)

4.89

Isoelectric Point (pI)

42.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo70_N PF20669 69 - 142 3.7e-12 Exocyst complex component Exo70 N-terminal
Exo70_C PF03081 266 - 643 2.7e-115 Exo70 exocyst complex subunit C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1816
AccBSI CCGCTC 1 cut(s) 1717
AccI GTMKAC 2 cut(s) 1352, 1858
AccII CGCG 2 cut(s) 1193, 1614
AciI CCGC 5 cut(s) 479, 1160, 1193, 1202, 1717
AclI AACGTT 1 cut(s) 1130
AclWI GGATC 3 cut(s) 489, 1065, 1935
AcsI RAATTY 2 cut(s) 1139, 1382
AcuI CTGAAG 1 cut(s) 636
AcyI GRCGYC 1 cut(s) 1817
AfaI GTAC 4 cut(s) 685, 1607, 1754, 1895
AfiI CCNNNNNNNGG 3 cut(s) 1089, 1184, 1676
AflIII ACRYGT 1 cut(s) 899
AjnI CCWGG 3 cut(s) 828, 1183, 1619
AloI GAACNNNNNNTCC 2 cut(s) 1179, 1211
Alw26I GTCTC 8 cut(s) 858, 870, 966, 1099, 1171, 1474, 1534, 1909
AlwI GGATC 3 cut(s) 489, 1065, 1935
AlwNI CAGNNNCTG 2 cut(s) 1664, 1688
Ama87I CYCGRG 1 cut(s) 1708
ApeKI GCWGC 4 cut(s) 44, 521, 1157, 1591
ApoI RAATTY 2 cut(s) 1139, 1382
AspLEI GCGC 3 cut(s) 1193, 1616, 1762
AspS9I GGNCC 2 cut(s) 968, 1667
AsuC2I CCSGG 3 cut(s) 1065, 1516, 1671
AsuHPI GGTGA 7 cut(s) 154, 200, 610, 962, 1190, 1365, 1840
AsuNHI GCTAGC 1 cut(s) 1015
AvaI CYCGRG 1 cut(s) 1708
AvaII GGWCC 2 cut(s) 968, 1667
BauI CACGAG 1 cut(s) 135
BbvCI CCTCAGC 1 cut(s) 150
BbvI GCAGC 4 cut(s) 31, 508, 1169, 1603
BccI CCATC 6 cut(s) 14, 760, 1318, 1330, 1646, 1764
BceAI ACGGC 1 cut(s) 410
BciT130I CCWGG 3 cut(s) 830, 1185, 1621
BciVI GTATCC 1 cut(s) 148
BcnI CCSGG 3 cut(s) 1065, 1516, 1671
BcoDI GTCTC 8 cut(s) 858, 870, 966, 1099, 1171, 1474, 1534, 1909
BfaI CTAG 2 cut(s) 234, 1016
BfuI GTATCC 1 cut(s) 148
BglII AGATCT 4 cut(s) 71, 619, 867, 1294
BisI GCNGC 4 cut(s) 45, 522, 1158, 1592
BlsI GCNGC 4 cut(s) 46, 523, 1159, 1593
Bme1390I CCNGG 6 cut(s) 830, 1065, 1185, 1516, 1621, 1671
Bme18I GGWCC 2 cut(s) 968, 1667
BmeT110I CYCGRG 1 cut(s) 1708
BmgT120I GGNCC 2 cut(s) 968, 1667
BmiI GGNNCC 5 cut(s) 924, 1565, 1625, 1669, 1721
BmrFI CCNGG 6 cut(s) 830, 1065, 1185, 1516, 1621, 1671
BmsI GCATC 8 cut(s) 286, 424, 691, 921, 1028, 1033, 1183, 1276
BmtI GCTAGC 1 cut(s) 1019
BpmI CTGGAG 1 cut(s) 1206
Bpu10I CCTNAGC 3 cut(s) 150, 992, 1681
BpuEI CTTGAG 4 cut(s) 419, 608, 862, 1628
BpuMI CCSGG 3 cut(s) 1065, 1516, 1671
Bsa29I ATCGAT 1 cut(s) 1041
BsaAI YACGTR 1 cut(s) 1216
BsaHI GRCGYC 1 cut(s) 1817
BsaJI CCNNGG 4 cut(s) 504, 1183, 1709, 1723
BsaWI WCCGGW 2 cut(s) 1375, 1766
BsaXI ACNNNNNCTCC 6 cut(s) 485, 515, 1179, 1209, 1349, 1379
Bsc4I CCNNNNNNNGG 3 cut(s) 1089, 1184, 1676
Bse3DI GCAATG 2 cut(s) 630, 1398
BseBI CCWGG 3 cut(s) 830, 1185, 1621
BseCI ATCGAT 1 cut(s) 1041
BseDI CCNNGG 4 cut(s) 504, 1183, 1709, 1723
BseGI GGATG 8 cut(s) 301, 409, 715, 771, 1060, 1168, 1174, 1775
BseLI CCNNNNNNNGG 3 cut(s) 1089, 1184, 1676
BseMI GCAATG 2 cut(s) 630, 1398
BseMII CTCAG 8 cut(s) 141, 173, 522, 588, 972, 1014, 1529, 1695
BseRI GAGGAG 4 cut(s) 359, 1117, 1357, 1481
BseXI GCAGC 4 cut(s) 31, 508, 1169, 1603
Bsh1236I CGCG 2 cut(s) 1193, 1614
BshVI ATCGAT 1 cut(s) 1041
BsiHKCI CYCGRG 1 cut(s) 1708
BsiSI CCGG 6 cut(s) 1065, 1316, 1376, 1516, 1671, 1767
BslFI GGGAC 2 cut(s) 593, 1653
BslI CCNNNNNNNGG 3 cut(s) 1089, 1184, 1676
BsmAI GTCTC 8 cut(s) 858, 870, 966, 1099, 1171, 1474, 1534, 1909
BsmFI GGGAC 2 cut(s) 593, 1653
BsoBI CYCGRG 1 cut(s) 1708
Bsp1407I TGTACA 2 cut(s) 683, 1752
Bsp19I CCATGG 1 cut(s) 504
BspACI CCGC 5 cut(s) 479, 1160, 1193, 1202, 1717
BspCNI CTCAG 8 cut(s) 142, 172, 523, 587, 973, 1015, 1530, 1694
BspDI ATCGAT 1 cut(s) 1041
BspFNI CGCG 2 cut(s) 1193, 1614
BspHI TCATGA 1 cut(s) 367
BspLI GGNNCC 5 cut(s) 924, 1565, 1625, 1669, 1721
BspOI GCTAGC 1 cut(s) 1019
BspPI GGATC 3 cut(s) 489, 1065, 1935
BsrBI CCGCTC 1 cut(s) 1717
BsrDI GCAATG 2 cut(s) 630, 1398
BsrGI TGTACA 2 cut(s) 683, 1752
BssECI CCNNGG 4 cut(s) 504, 1183, 1709, 1723
BssNI GRCGYC 1 cut(s) 1817
BssSI CACGAG 1 cut(s) 135
BssT1I CCWWGG 2 cut(s) 504, 1723
Bst2BI CACGAG 1 cut(s) 135
Bst2UI CCWGG 3 cut(s) 830, 1185, 1621
Bst4CI ACNGT 4 cut(s) 665, 916, 1856, 1871
Bst6I CTCTTC 5 cut(s) 21, 492, 1272, 1341, 1375
BstACI GRCGYC 1 cut(s) 1817
BstAUI TGTACA 2 cut(s) 683, 1752
BstBAI YACGTR 1 cut(s) 1216
BstC8I GCNNGC 2 cut(s) 1017, 1596
BstDSI CCRYGG 1 cut(s) 504
BstF5I GGATG 8 cut(s) 301, 409, 715, 771, 1060, 1168, 1174, 1775
BstFNI CGCG 2 cut(s) 1193, 1614
BstHHI GCGC 3 cut(s) 1193, 1616, 1762
BstMAI GTCTC 8 cut(s) 858, 870, 966, 1099, 1171, 1474, 1534, 1909
BstMWI GCNNNNNNNGC 2 cut(s) 445, 1157
BstNI CCWGG 3 cut(s) 830, 1185, 1621
BstNSI RCATGY 1 cut(s) 903
BstSCI CCNGG 6 cut(s) 828, 1063, 1183, 1514, 1619, 1669
BstUI CGCG 2 cut(s) 1193, 1614
BstV1I GCAGC 4 cut(s) 31, 508, 1169, 1603
BstX2I RGATCY 5 cut(s) 71, 619, 867, 1294, 1927
BstYI RGATCY 5 cut(s) 71, 619, 867, 1294, 1927
Bsu15I ATCGAT 1 cut(s) 1041
BsuI GTATCC 1 cut(s) 148
BsuTUI ATCGAT 1 cut(s) 1041
BtgI CCRYGG 1 cut(s) 504
BtgZI GCGATG 2 cut(s) 1155, 1296
BtsCI GGATG 8 cut(s) 301, 409, 715, 771, 1060, 1168, 1174, 1775
BtsI GCAGTG 1 cut(s) 1734
BtsIMutI CAGTG 3 cut(s) 1734, 1876, 1882
Cac8I GCNNGC 2 cut(s) 1017, 1596
CaiI CAGNNNCTG 2 cut(s) 1664, 1688
CciI TCATGA 1 cut(s) 367
CfoI GCGC 3 cut(s) 1193, 1616, 1762
Cfr13I GGNCC 2 cut(s) 968, 1667
ClaI ATCGAT 1 cut(s) 1041
CseI GACGC 1 cut(s) 1806
Csp6I GTAC 4 cut(s) 684, 1606, 1753, 1894
CviQI GTAC 4 cut(s) 684, 1606, 1753, 1894
DrdI GACNNNNNNGTC 1 cut(s) 1816
DseDI GACNNNNNNGTC 1 cut(s) 1816
Eam1104I CTCTTC 5 cut(s) 21, 492, 1272, 1341, 1375
EarI CTCTTC 5 cut(s) 21, 492, 1272, 1341, 1375
Eco130I CCWWGG 2 cut(s) 504, 1723
Eco32I GATATC 1 cut(s) 823
Eco47I GGWCC 2 cut(s) 968, 1667
Eco57I CTGAAG 1 cut(s) 636
Eco88I CYCGRG 1 cut(s) 1708
EcoRI GAATTC 1 cut(s) 1139
EcoRII CCWGG 3 cut(s) 828, 1183, 1619
EcoRV GATATC 1 cut(s) 823
EcoT14I CCWWGG 2 cut(s) 504, 1723
ErhI CCWWGG 2 cut(s) 504, 1723
FalI AAGNNNNNCTT 2 cut(s) 1422, 1454
FaqI GGGAC 2 cut(s) 593, 1653
FauI CCCGC 1 cut(s) 1209
FblI GTMKAC 2 cut(s) 1352, 1858
Fnu4HI GCNGC 4 cut(s) 45, 522, 1158, 1592
FokI GGATG 8 cut(s) 308, 416, 722, 778, 1047, 1161, 1175, 1782
Fsp4HI GCNGC 4 cut(s) 45, 522, 1158, 1592
FspBI CTAG 2 cut(s) 234, 1016
GlaI GCGC 3 cut(s) 1192, 1615, 1761
GluI GCNGC 4 cut(s) 45, 522, 1158, 1592
GsuI CTGGAG 1 cut(s) 1206
HapII CCGG 6 cut(s) 1065, 1316, 1376, 1516, 1671, 1767
HgaI GACGC 1 cut(s) 1806
HhaI GCGC 3 cut(s) 1193, 1616, 1762
Hin1I GRCGYC 1 cut(s) 1817
Hin6I GCGC 3 cut(s) 1191, 1614, 1760
HinP1I GCGC 3 cut(s) 1191, 1614, 1760
HpaII CCGG 6 cut(s) 1065, 1316, 1376, 1516, 1671, 1767
HphI GGTGA 7 cut(s) 154, 200, 610, 962, 1190, 1365, 1840
Hpy166II GTNNAC 7 cut(s) 684, 971, 1198, 1353, 1753, 1859, 1896
Hpy188I TCNGA 6 cut(s) 274, 867, 927, 1024, 1534, 1783
Hpy8I GTNNAC 7 cut(s) 684, 971, 1198, 1353, 1753, 1859, 1896
Hpy99I CGWCG 3 cut(s) 463, 781, 911
HpyAV CCTTC 3 cut(s) 272, 913, 1448
HpyCH4III ACNGT 4 cut(s) 665, 916, 1856, 1871
HpyCH4IV ACGT 4 cut(s) 582, 779, 1130, 1215
HpyCH4V TGCA 4 cut(s) 934, 1121, 1157, 1289
HpyF10VI GCNNNNNNNGC 2 cut(s) 445, 1157
HpySE526I ACGT 4 cut(s) 582, 779, 1130, 1215
Hsp92I GRCGYC 1 cut(s) 1817
HspAI GCGC 3 cut(s) 1191, 1614, 1760
LmnI GCTCC 4 cut(s) 346, 928, 1629, 1714
Lsp1109I GCAGC 4 cut(s) 31, 508, 1169, 1603
LweI GCATC 8 cut(s) 286, 424, 691, 921, 1028, 1033, 1183, 1276
MaeI CTAG 2 cut(s) 234, 1016
MaeII ACGT 4 cut(s) 582, 779, 1130, 1215
MbiI CCGCTC 1 cut(s) 1717
MflI RGATCY 5 cut(s) 71, 619, 867, 1294, 1927
MluCI AATT 7 cut(s) 943, 986, 1139, 1222, 1382, 1776, 1960
MlyI GAGTC 5 cut(s) 91, 509, 1256, 1358, 1911
MmeI TCCRAC 1 cut(s) 1905
MseI TTAA 5 cut(s) 60, 207, 356, 411, 942
MslI CAYNNNNRTG 2 cut(s) 1284, 1826
MspA1I CMGCKG 1 cut(s) 1160
MspI CCGG 6 cut(s) 1065, 1316, 1376, 1516, 1671, 1767
MspR9I CCNGG 6 cut(s) 830, 1065, 1185, 1516, 1621, 1671
MvaI CCWGG 3 cut(s) 830, 1185, 1621
MvnI CGCG 2 cut(s) 1193, 1614
MwoI GCNNNNNNNGC 2 cut(s) 445, 1157
NciI CCSGG 3 cut(s) 1065, 1516, 1671
NcoI CCATGG 1 cut(s) 504
NheI GCTAGC 1 cut(s) 1015
NlaIV GGNNCC 5 cut(s) 924, 1565, 1625, 1669, 1721
NspI RCATGY 1 cut(s) 903
PagI TCATGA 1 cut(s) 367
PciI ACATGT 1 cut(s) 899
PcsI WCGNNNNNNNCGW 1 cut(s) 785
PfeI GAWTC 7 cut(s) 248, 541, 601, 997, 1699, 1728, 1831
PkrI GCNGC 4 cut(s) 46, 523, 1159, 1593
PleI GAGTC 5 cut(s) 90, 508, 1256, 1357, 1911
PpsI GAGTC 5 cut(s) 90, 508, 1256, 1357, 1911
Ppu21I YACGTR 1 cut(s) 1216
PscI ACATGT 1 cut(s) 899
Psp1406I AACGTT 1 cut(s) 1130
Psp6I CCWGG 3 cut(s) 828, 1183, 1619
PspGI CCWGG 3 cut(s) 828, 1183, 1619
PspN4I GGNNCC 5 cut(s) 924, 1565, 1625, 1669, 1721
PspPI GGNCC 2 cut(s) 968, 1667
PstNI CAGNNNCTG 2 cut(s) 1664, 1688
PsuI RGATCY 5 cut(s) 71, 619, 867, 1294, 1927
RsaI GTAC 4 cut(s) 685, 1607, 1754, 1895
RsaNI GTAC 4 cut(s) 684, 1606, 1753, 1894
RseI CAYNNNNRTG 2 cut(s) 1284, 1826
SaqAI TTAA 5 cut(s) 60, 207, 356, 411, 942
SatI GCNGC 4 cut(s) 45, 522, 1158, 1592
Sau96I GGNCC 2 cut(s) 968, 1667
SchI GAGTC 5 cut(s) 91, 509, 1256, 1358, 1911
ScrFI CCNGG 6 cut(s) 830, 1065, 1185, 1516, 1621, 1671
SfaNI GCATC 8 cut(s) 286, 424, 691, 921, 1028, 1033, 1183, 1276
SinI GGWCC 2 cut(s) 968, 1667
SmiMI CAYNNNNRTG 2 cut(s) 1284, 1826
SmlI CTYRAG 4 cut(s) 398, 623, 841, 1643
SmoI CTYRAG 4 cut(s) 398, 623, 841, 1643
Sse9I AATT 7 cut(s) 943, 986, 1139, 1222, 1382, 1776, 1960
SsiI CCGC 5 cut(s) 479, 1160, 1193, 1202, 1717
SspMI CTAG 2 cut(s) 234, 1016
StyD4I CCNGG 6 cut(s) 828, 1063, 1183, 1514, 1619, 1669
StyI CCWWGG 2 cut(s) 504, 1723
TaaI ACNGT 4 cut(s) 665, 916, 1856, 1871
TaiI ACGT 4 cut(s) 585, 782, 1133, 1218
TaqI TCGA 7 cut(s) 243, 909, 1041, 1260, 1341, 1428, 1743
TaqII GACCGA 2 cut(s) 456, 881
TasI AATT 7 cut(s) 943, 986, 1139, 1222, 1382, 1776, 1960
TatI WGTACW 3 cut(s) 683, 1752, 1893
TfiI GAWTC 7 cut(s) 248, 541, 601, 997, 1699, 1728, 1831
Tru1I TTAA 5 cut(s) 60, 207, 356, 411, 942
Tru9I TTAA 5 cut(s) 60, 207, 356, 411, 942
TscAI CASTG 3 cut(s) 1741, 1876, 1882
TseI GCWGC 4 cut(s) 44, 521, 1157, 1591
TspDTI ATGAA 4 cut(s) 195, 261, 291, 1235
TspGWI ACGGA 2 cut(s) 351, 1063
TspRI CASTG 3 cut(s) 1741, 1876, 1882
VpaK11BI GGWCC 2 cut(s) 968, 1667
XapI RAATTY 2 cut(s) 1139, 1382
XceI RCATGY 1 cut(s) 903
XmiI GTMKAC 2 cut(s) 1352, 1858
XspI CTAG 2 cut(s) 234, 1016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.