MD17G1059500.v1.1

exocyst complex component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0009068
Physical Location & Seq
Reverse (-)
4853058 .. 4853735
678 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1059500.v1.1.491

Sequence Viewer

Length: 471 bp
ATGCTGCAGCTTCTGAATTTTGGTGAAGCCATGTCTATTGGCCCCCACCAACCGGAAAAGTTGTTCCGCATTCTTGACATGTATGAGGTGCTTGCAGATGTTCTTCCCGACATAGATGCTTTATACGCAGACGAGGCTGGTTCTTCTTGCTTGATGCTGATTCCATTTCGCTGTCGCCTGACACGAGTCCTACCTCAGACGAGGAGAACAAACCCACTGATTCTCCGGGCAGAATTTTCCCAATGGTTCGCCACTTCCGATCACTTACTTCAACTCTGGAAGGCAACCTTGACGAAAAAGTCCAGGTTATACAAGGATCCTTCCTTGCAACACGTATTTTTGATGAACAATCTACGGTACATGGCTCAGAAGGTTAAGGGGGATGAACTGAGGCCCATTTTCGGAGACGGCTGGCTTCGGAAGTGCAACGGGAAAAGTTCAACAGCAAGCATTAAACTACCAGAGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0006887 GO:0008150 GO:0009987 GO:0016192 GO:0032940 GO:0046903 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
ko04910 map04910
Pfam Domains
Protein Families
Exo70

Protein Analysis

157

Amino Acids

17.97

Weight (kDa)

9.24

Isoelectric Point (pI)

51.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo70_C PF03081 1 - 47 8.9e-10 Exo70 exocyst complex subunit C-terminal
Exo70_C PF03081 89 - 145 3.1e-16 Exo70 exocyst complex subunit C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 298
AciI CCGC 1 cut(s) 67
AclWI GGATC 2 cut(s) 311, 324
AcsI RAATTY 2 cut(s) 16, 233
AfaI GTAC 1 cut(s) 359
AfiI CCNNNNNNNGG 2 cut(s) 52, 401
AflIII ACRYGT 2 cut(s) 78, 331
AgsI TTSAA 2 cut(s) 272, 441
AjnI CCWGG 1 cut(s) 302
AloI GAACNNNNNNTCC 2 cut(s) 47, 79
AluBI AGCT 1 cut(s) 10
AluI AGCT 1 cut(s) 10
Alw26I GTCTC 1 cut(s) 399
AlwI GGATC 2 cut(s) 311, 324
AlwNI CAGNNNCTG 1 cut(s) 13
AoxI GGCC 2 cut(s) 40, 392
ApeKI GCWGC 2 cut(s) 4, 7
ApoI RAATTY 2 cut(s) 16, 233
AspS9I GGNCC 2 cut(s) 41, 393
AsuC2I CCSGG 1 cut(s) 227
AsuHPI GGTGA 1 cut(s) 35
BamHI GGATCC 1 cut(s) 316
BauI CACGAG 1 cut(s) 183
BbvI GCAGC 1 cut(s) 19
BceAI ACGGC 1 cut(s) 424
BcgI CGANNNNNNTGC 2 cut(s) 98, 132
BciT130I CCWGG 1 cut(s) 304
BcnI CCSGG 1 cut(s) 227
BcoDI GTCTC 1 cut(s) 399
BfaI CTAG 1 cut(s) 469
BfmI CTRYAG 1 cut(s) 5
BglII AGATCT 1 cut(s) 465
BisI GCNGC 2 cut(s) 5, 8
BlsI GCNGC 2 cut(s) 6, 9
Bme1390I CCNGG 2 cut(s) 227, 304
BmgT120I GGNCC 2 cut(s) 41, 393
BmiI GGNNCC 2 cut(s) 43, 318
BmrFI CCNGG 2 cut(s) 227, 304
BmsI GCATC 2 cut(s) 106, 144
BoxI GACNNNNGTC 1 cut(s) 185
BpuMI CCSGG 1 cut(s) 227
BsaAI YACGTR 1 cut(s) 334
BsaWI WCCGGW 1 cut(s) 52
BsaXI ACNNNNNCTCC 2 cut(s) 207, 237
Bsc4I CCNNNNNNNGG 2 cut(s) 52, 401
BseBI CCWGG 1 cut(s) 304
BseGI GGATG 1 cut(s) 388
BseLI CCNNNNNNNGG 2 cut(s) 52, 401
BseMII CTCAG 3 cut(s) 209, 380, 380
BseRI GAGGAG 1 cut(s) 217
BseXI GCAGC 1 cut(s) 19
BshFI GGCC 2 cut(s) 42, 394
BsiSI CCGG 2 cut(s) 53, 226
BslI CCNNNNNNNGG 2 cut(s) 52, 401
BsmAI GTCTC 1 cut(s) 399
BsmBI CGTCTC 1 cut(s) 399
BsmI GAATGC 1 cut(s) 69
BsnI GGCC 2 cut(s) 42, 394
Bsp143I GATC 3 cut(s) 259, 316, 465
BspACI CCGC 1 cut(s) 67
BspANI GGCC 2 cut(s) 42, 394
BspCNI CTCAG 3 cut(s) 208, 379, 381
BspLI GGNNCC 2 cut(s) 43, 318
BspMAI CTGCAG 1 cut(s) 9
BspPI GGATC 2 cut(s) 311, 324
BssMI GATC 3 cut(s) 259, 316, 465
BssSI CACGAG 1 cut(s) 183
Bst2BI CACGAG 1 cut(s) 183
Bst2UI CCWGG 1 cut(s) 304
Bst4CI ACNGT 1 cut(s) 357
BstBAI YACGTR 1 cut(s) 334
BstC8I GCNNGC 3 cut(s) 93, 413, 448
BstDEI CTNAG 3 cut(s) 195, 366, 389
BstF5I GGATG 1 cut(s) 388
BstKTI GATC 3 cut(s) 262, 319, 468
BstMAI GTCTC 1 cut(s) 399
BstMBI GATC 3 cut(s) 259, 316, 465
BstMWI GCNNNNNNNGC 2 cut(s) 125, 134
BstNI CCWGG 1 cut(s) 304
BstNSI RCATGY 1 cut(s) 82
BstPAI GACNNNNGTC 1 cut(s) 185
BstSCI CCNGG 2 cut(s) 225, 302
BstSFI CTRYAG 1 cut(s) 5
BstV1I GCAGC 1 cut(s) 19
BstX2I RGATCY 2 cut(s) 316, 465
BstYI RGATCY 2 cut(s) 316, 465
BsuRI GGCC 2 cut(s) 42, 394
BtsCI GGATG 1 cut(s) 388
BtsIMutI CAGTG 1 cut(s) 215
Cac8I GCNNGC 3 cut(s) 93, 413, 448
CaiI CAGNNNCTG 1 cut(s) 13
Cfr13I GGNCC 2 cut(s) 41, 393
Csp6I GTAC 1 cut(s) 358
CviAII CATG 3 cut(s) 31, 79, 361
CviJI RGCY 8 cut(s) 10, 29, 42, 137, 365, 394, 411, 415
CviKI_1 RGCY 8 cut(s) 10, 29, 42, 137, 365, 394, 411, 415
CviQI GTAC 1 cut(s) 358
DdeI CTNAG 3 cut(s) 195, 366, 389
DpnI GATC 3 cut(s) 261, 318, 467
DpnII GATC 3 cut(s) 259, 316, 465
DrdI GACNNNNNNGTC 1 cut(s) 298
DseDI GACNNNNNNGTC 1 cut(s) 298
EcoRII CCWGG 1 cut(s) 302
Esp3I CGTCTC 1 cut(s) 399
FaeI CATG 3 cut(s) 34, 82, 364
FaiI YATR 7 cut(s) 32, 80, 84, 113, 124, 310, 362
FalI AAGNNNNNCTT 2 cut(s) 272, 304
FatI CATG 3 cut(s) 30, 78, 360
Fnu4HI GCNGC 2 cut(s) 5, 8
FokI GGATG 1 cut(s) 395
Fsp4HI GCNGC 2 cut(s) 5, 8
FspBI CTAG 1 cut(s) 469
GluI GCNGC 2 cut(s) 5, 8
HaeIII GGCC 2 cut(s) 42, 394
HapII CCGG 2 cut(s) 53, 226
Hin1II CATG 3 cut(s) 34, 82, 364
HinfI GANTC 3 cut(s) 160, 186, 220
HpaII CCGG 2 cut(s) 53, 226
HphI GGTGA 1 cut(s) 35
Hpy188I TCNGA 6 cut(s) 15, 198, 259, 369, 404, 420
Hpy188III TCNNGA 3 cut(s) 74, 107, 277
HpyAV CCTTC 3 cut(s) 274, 330, 364
HpyCH4III ACNGT 1 cut(s) 357
HpyCH4IV ACGT 1 cut(s) 333
HpyCH4V TGCA 4 cut(s) 7, 95, 328, 426
HpyF10VI GCNNNNNNNGC 2 cut(s) 125, 134
HpyF3I CTNAG 3 cut(s) 195, 366, 389
HpySE526I ACGT 1 cut(s) 333
Hsp92II CATG 3 cut(s) 34, 82, 364
Kzo9I GATC 3 cut(s) 259, 316, 465
LpnPI CCDG 8 cut(s) 66, 123, 191, 239, 262, 289, 316, 397
Lsp1109I GCAGC 1 cut(s) 19
LweI GCATC 2 cut(s) 106, 144
MaeI CTAG 1 cut(s) 469
MaeII ACGT 1 cut(s) 333
MalI GATC 3 cut(s) 261, 318, 467
MboI GATC 3 cut(s) 259, 316, 465
MboII GAAGA 2 cut(s) 95, 135
MflI RGATCY 2 cut(s) 316, 465
MluCI AATT 2 cut(s) 16, 233
MlyI GAGTC 1 cut(s) 195
MnlI CCTC 5 cut(s) 79, 127, 195, 204, 384
MseI TTAA 2 cut(s) 375, 453
MspI CCGG 2 cut(s) 53, 226
MspR9I CCNGG 2 cut(s) 227, 304
Mva1269I GAATGC 1 cut(s) 69
MvaI CCWGG 1 cut(s) 304
MwoI GCNNNNNNNGC 2 cut(s) 125, 134
NciI CCSGG 1 cut(s) 227
NdeII GATC 3 cut(s) 259, 316, 465
NlaIII CATG 3 cut(s) 34, 82, 364
NlaIV GGNNCC 2 cut(s) 43, 318
NspI RCATGY 1 cut(s) 82
PciI ACATGT 1 cut(s) 78
PcsI WCGNNNNNNNCGW 2 cut(s) 181, 255
PctI GAATGC 1 cut(s) 69
PfeI GAWTC 2 cut(s) 160, 220
PkrI GCNGC 2 cut(s) 6, 9
PleI GAGTC 1 cut(s) 194
PpsI GAGTC 1 cut(s) 194
Ppu21I YACGTR 1 cut(s) 334
PscI ACATGT 1 cut(s) 78
PshAI GACNNNNGTC 1 cut(s) 185
Psp6I CCWGG 1 cut(s) 302
PspGI CCWGG 1 cut(s) 302
PspN4I GGNNCC 2 cut(s) 43, 318
PspPI GGNCC 2 cut(s) 41, 393
PstI CTGCAG 1 cut(s) 9
PstNI CAGNNNCTG 1 cut(s) 13
PsuI RGATCY 2 cut(s) 316, 465
RsaI GTAC 1 cut(s) 359
RsaNI GTAC 1 cut(s) 358
SaqAI TTAA 2 cut(s) 375, 453
SatI GCNGC 2 cut(s) 5, 8
Sau3AI GATC 3 cut(s) 259, 316, 465
Sau96I GGNCC 2 cut(s) 41, 393
SchI GAGTC 1 cut(s) 195
ScrFI CCNGG 2 cut(s) 227, 304
SetI ASST 7 cut(s) 12, 90, 196, 290, 308, 336, 375
SfaNI GCATC 2 cut(s) 106, 144
SfcI CTRYAG 1 cut(s) 5
Sse9I AATT 2 cut(s) 16, 233
SsiI CCGC 1 cut(s) 67
SspMI CTAG 1 cut(s) 469
StyD4I CCNGG 2 cut(s) 225, 302
TaaI ACNGT 1 cut(s) 357
TaiI ACGT 1 cut(s) 336
TasI AATT 2 cut(s) 16, 233
TfiI GAWTC 2 cut(s) 160, 220
Tru1I TTAA 2 cut(s) 375, 453
Tru9I TTAA 2 cut(s) 375, 453
TscAI CASTG 1 cut(s) 222
TseI GCWGC 2 cut(s) 4, 7
TspDTI ATGAA 2 cut(s) 359, 399
TspRI CASTG 1 cut(s) 222
XapI RAATTY 2 cut(s) 16, 233
XceI RCATGY 1 cut(s) 82
XspI CTAG 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.