AT3G43540

Protein of unknown function (DUF1350)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0013928
Physical Location & Seq
Forward (+)
15430660 .. 15433355
2696 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G43540.1

Sequence Viewer

Length: 1122 bp
ATGGAGTTTGGATTAGCCACTTCGAGTTTTCTGGGTACTCAACTAATCCCGGCCACTCTGTTTAGCAAACGAGCTCGAGCTTTGTCTCTCACCGTCACAGCGACCGGTGGCAGCAGAAACAATAAGGTTTACACGAGGCTTGATTCTTGTCTAGTGATTCCTCCTCCTCCTAAATTCAAGAAACCGCGTGCTATTGTTAAATTCTTGGGAGGTGCCTTCGTCGGAGCTGTTCCTGAACTCACTTACAGTTACCTTAAGGAGCTACTGGCAAAGGAAGGGTATTTGATAGTATCTGTGCCGTACAATGTTACATTTGATCATGAACAAGCTGCTAAGCAAGTTTATGAGAGGTTTAATTCATGCTTGGACACGATTGTCTCATCTGGAATTCCAAATTCAAACTTGAAGCCTGGAGATTTAGCTGACCTTCCTCTTTTCTCAGTTGGTCACAGTAATGGTGCACTGCTCCAAGTTTTGACCGGAAGTTATTTTGCTGAGAAGATACCAAAGGTTAATGCCATTATTTCATTCAATAACAAATCAGCGACAGAGGCGGTTCCATATTTTGAGCAGCTTGGTCCTCTCATCCAGCAGATGCTGCCAATGGTGGAAGCTTCTCCAGTATATGAAATGGCTCGAAATGCTTCAGGTGATGTTTTGAAACTCTTACTTGACACAGCTGGGACAACGATTCTAAACAACGATCAAGAAGCTCTTAAGTCATTTACTAAATTGGTGGATCAATTGCCATCAGTATTTGGTGAGGTAGGACAAGGAGTGTCAGAATTCAGACCGTCACCACTTGAGAATCGCAATTGCTTCAAGTGCTCCTACAGCGTTCCACACACTCTACTGGTACAATTCAACTCCGATGCAATCGACGAGACTGATTTGCTCGAAGAAACTCTTAGGCCTCGGATAGAATCTATTGGTGGTACACTTGAAAAGGTTCGGCTGAACGGAAACCATCTTACACCTTGCATACAGGATCCAAAGTGGCAAATTGGGACTGTGTACACGCCAGCAGACGCTGTGGCTCAGGCGCTAAAGACCATTCCTCTTAGCGAAACTCGAGTTCTTTCCAGGACGATCGTTGACTGGTTTAGACGGTTTGAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1350

Protein Analysis

373

Amino Acids

40.98

Weight (kDa)

6.47

Isoelectric Point (pI)

27.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1350 PF07082 60 - 368 5.1e-48 Protein of unknown function (DUF1350)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013928)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G43540 AT3G43540
fragaria_vesca FvH4_2g37050 FvH4_2g37050
malus_domestica MD08G1047000.v1.1
pyrus_communis pycom08g03940
rosa_chinensis RchiOBHm_Chr6g0304731
rosa_laevigata RLG00000010955
rosa_multiflora Rmu_co8515209.1_g000001
rosa_roxburghii Rroxscaffold_7G00163550
rosa_rugosa Rorug06G0333900 Rorug06G0334000
rosa_samantha Rh6AG445900 Rh6BG455800 Rh6CG459000 Rh6DG446000
rosa_wichuraiana Rw6G038790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 374
AccB1I GGYRCC 1 cut(s) 212
AccII CGCG 1 cut(s) 187
AciI CCGC 2 cut(s) 185, 554
AclWI GGATC 3 cut(s) 747, 983, 996
AcoI YGGCCR 1 cut(s) 51
AcsI RAATTY 5 cut(s) 173, 200, 387, 394, 785
AcuI CTGAAG 1 cut(s) 630
AfaI GTAC 5 cut(s) 37, 302, 858, 937, 1016
AflII CTTAAG 2 cut(s) 254, 716
AgeI ACCGGT 1 cut(s) 104
AgsI TTSAA 8 cut(s) 178, 399, 406, 532, 661, 823, 865, 944
AjnI CCWGG 2 cut(s) 409, 1082
Alw21I GWGCWC 3 cut(s) 76, 463, 830
Alw26I GTCTC 3 cut(s) 90, 382, 878
Alw44I GTGCAC 1 cut(s) 459
AlwI GGATC 3 cut(s) 747, 983, 996
AlwNI CAGNNNCTG 2 cut(s) 598, 1031
Ama87I CYCGRG 2 cut(s) 75, 1071
AoxI GGCC 2 cut(s) 51, 911
ApaLI GTGCAC 1 cut(s) 459
ApeKI GCWGC 4 cut(s) 111, 329, 571, 598
ApoI RAATTY 5 cut(s) 173, 200, 387, 394, 785
AsiGI ACCGGT 1 cut(s) 104
Asp700I GAANNNNTTC 2 cut(s) 643, 948
AspLEI GCGC 1 cut(s) 1045
AspS9I GGNCC 1 cut(s) 578
AsuC2I CCSGG 1 cut(s) 50
AsuHPI GGTGA 4 cut(s) 82, 662, 773, 789
AvaI CYCGRG 2 cut(s) 75, 1071
AvaII GGWCC 1 cut(s) 578
BaeGI GKGCMC 1 cut(s) 463
BamHI GGATCC 1 cut(s) 988
BanI GGYRCC 1 cut(s) 212
BanII GRGCYC 1 cut(s) 76
BauI CACGAG 1 cut(s) 133
Bbv12I GWGCWC 3 cut(s) 76, 463, 830
BbvI GCAGC 4 cut(s) 123, 316, 583, 585
BccI CCATC 2 cut(s) 757, 975
BceAI ACGGC 1 cut(s) 283
BciT130I CCWGG 2 cut(s) 411, 1084
BclI TGATCA 1 cut(s) 316
BcnI CCSGG 1 cut(s) 50
BcoDI GTCTC 3 cut(s) 90, 382, 878
BfaI CTAG 1 cut(s) 152
BfmI CTRYAG 1 cut(s) 832
BfoI RGCGCY 1 cut(s) 1046
BfrI CTTAAG 2 cut(s) 254, 716
BisI GCNGC 4 cut(s) 112, 330, 572, 599
BlpI GCTNAGC 1 cut(s) 333
BlsI GCNGC 4 cut(s) 113, 331, 573, 600
Bme1390I CCNGG 3 cut(s) 50, 411, 1084
Bme18I GGWCC 1 cut(s) 578
BmeT110I CYCGRG 2 cut(s) 75, 1071
BmgT120I GGNCC 1 cut(s) 578
BmiI GGNNCC 3 cut(s) 214, 558, 990
BmrFI CCNGG 3 cut(s) 50, 411, 1084
BmsI GCATC 2 cut(s) 585, 862
BpmI CTGGAG 2 cut(s) 432, 603
Bpu10I CCTNAGC 1 cut(s) 1038
Bpu1102I GCTNAGC 1 cut(s) 333
BpuEI CTTGAG 1 cut(s) 824
BpuMI CCSGG 1 cut(s) 50
BsaJI CCNNGG 1 cut(s) 914
BsaWI WCCGGW 2 cut(s) 104, 479
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 4 cut(s) 270, 620, 858, 1103
BseBI CCWGG 2 cut(s) 411, 1084
BseDI CCNNGG 1 cut(s) 914
BseGI GGATG 1 cut(s) 585
BseMII CTCAG 3 cut(s) 453, 486, 1052
BseNI ACTGG 4 cut(s) 270, 620, 858, 1103
BseRI GAGGAG 2 cut(s) 153, 156
BseSI GKGCMC 1 cut(s) 463
BseXI GCAGC 4 cut(s) 123, 316, 583, 585
BseYI CCCAGC 1 cut(s) 680
Bsh1236I CGCG 1 cut(s) 187
Bsh1285I CGRYCG 2 cut(s) 105, 1092
BshFI GGCC 2 cut(s) 53, 913
BshNI GGYRCC 1 cut(s) 212
BshTI ACCGGT 1 cut(s) 104
BsiEI CGRYCG 2 cut(s) 105, 1092
BsiHKAI GWGCWC 3 cut(s) 76, 463, 830
BsiHKCI CYCGRG 2 cut(s) 75, 1071
BsiSI CCGG 3 cut(s) 50, 105, 480
BslFI GGGAC 2 cut(s) 697, 1021
BsmAI GTCTC 3 cut(s) 90, 382, 878
BsmFI GGGAC 2 cut(s) 697, 1021
BsnI GGCC 2 cut(s) 53, 913
BsoBI CYCGRG 2 cut(s) 75, 1071
Bsp1286I GDGCHC 3 cut(s) 76, 463, 830
Bsp1407I TGTACA 1 cut(s) 1014
Bsp143I GATC 5 cut(s) 316, 703, 739, 988, 1089
Bsp1720I GCTNAGC 1 cut(s) 333
BspACI CCGC 2 cut(s) 185, 554
BspANI GGCC 2 cut(s) 53, 913
BspCNI CTCAG 3 cut(s) 452, 487, 1051
BspFNI CGCG 1 cut(s) 187
BspHI TCATGA 1 cut(s) 319
BspLI GGNNCC 3 cut(s) 214, 558, 990
BspPI GGATC 3 cut(s) 747, 983, 996
BspT107I GGYRCC 1 cut(s) 212
BspTI CTTAAG 2 cut(s) 254, 716
BsrFI RCCGGY 1 cut(s) 104
BsrGI TGTACA 1 cut(s) 1014
BsrI ACTGG 4 cut(s) 270, 620, 858, 1103
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 1 cut(s) 914
BssMI GATC 5 cut(s) 316, 703, 739, 988, 1089
BssSI CACGAG 1 cut(s) 133
Bst2BI CACGAG 1 cut(s) 133
Bst2UI CCWGG 2 cut(s) 411, 1084
Bst4CI ACNGT 6 cut(s) 94, 248, 452, 795, 1012, 1110
BstAFI CTTAAG 2 cut(s) 254, 716
BstAPI GCANNNNNTGC 1 cut(s) 598
BstAUI TGTACA 1 cut(s) 1014
BstC8I GCNNGC 2 cut(s) 189, 1023
BstDEI CTNAG 6 cut(s) 333, 439, 495, 908, 1038, 1061
BstF5I GGATG 1 cut(s) 585
BstFNI CGCG 1 cut(s) 187
BstH2I RGCGCY 1 cut(s) 1046
BstHHI GCGC 1 cut(s) 1045
BstKTI GATC 5 cut(s) 319, 706, 742, 991, 1092
BstMAI GTCTC 3 cut(s) 90, 382, 878
BstMBI GATC 5 cut(s) 316, 703, 739, 988, 1089
BstMCI CGRYCG 2 cut(s) 105, 1092
BstMWI GCNNNNNNNGC 5 cut(s) 551, 598, 641, 825, 834
BstNI CCWGG 2 cut(s) 411, 1084
BstSCI CCNGG 3 cut(s) 48, 409, 1082
BstSFI CTRYAG 1 cut(s) 832
BstSLI GKGCMC 1 cut(s) 463
BstUI CGCG 1 cut(s) 187
BstV1I GCAGC 4 cut(s) 123, 316, 583, 585
BstX2I RGATCY 1 cut(s) 988
BstYI RGATCY 1 cut(s) 988
BsuRI GGCC 2 cut(s) 53, 913
BtsCI GGATG 1 cut(s) 585
BtsI GCAGTG 1 cut(s) 461
BtsIMutI CAGTG 1 cut(s) 461
Cac8I GCNNGC 2 cut(s) 189, 1023
CaiI CAGNNNCTG 2 cut(s) 598, 1031
CciI TCATGA 1 cut(s) 319
CfoI GCGC 1 cut(s) 1045
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 1 cut(s) 578
CseI GACGC 1 cut(s) 1037
Csp6I GTAC 5 cut(s) 36, 301, 857, 936, 1015
CspAI ACCGGT 1 cut(s) 104
CviAII CATG 2 cut(s) 320, 360
CviQI GTAC 5 cut(s) 36, 301, 857, 936, 1015
DdeI CTNAG 6 cut(s) 333, 439, 495, 908, 1038, 1061
DpnI GATC 5 cut(s) 318, 705, 741, 990, 1091
DpnII GATC 5 cut(s) 316, 703, 739, 988, 1089
DrdI GACNNNNNNGTC 1 cut(s) 374
DseDI GACNNNNNNGTC 1 cut(s) 374
EaeI YGGCCR 1 cut(s) 51
Ecl136II GAGCTC 1 cut(s) 74
Eco147I AGGCCT 1 cut(s) 913
Eco24I GRGCYC 1 cut(s) 76
Eco47I GGWCC 1 cut(s) 578
Eco53kI GAGCTC 1 cut(s) 74
Eco57I CTGAAG 1 cut(s) 630
Eco88I CYCGRG 2 cut(s) 75, 1071
EcoICRI GAGCTC 1 cut(s) 74
EcoRI GAATTC 2 cut(s) 387, 785
EcoRII CCWGG 2 cut(s) 409, 1082
EcoT38I GRGCYC 1 cut(s) 76
FaeI CATG 2 cut(s) 323, 363
FaiI YATR 7 cut(s) 321, 345, 361, 562, 625, 627, 983
FalI AAGNNNNNCTT 4 cut(s) 699, 731, 891, 923
FaqI GGGAC 2 cut(s) 697, 1021
FatI CATG 2 cut(s) 319, 359
FbaI TGATCA 1 cut(s) 316
Fnu4HI GCNGC 4 cut(s) 112, 330, 572, 599
FokI GGATG 1 cut(s) 572
FriOI GRGCYC 1 cut(s) 76
Fsp4HI GCNGC 4 cut(s) 112, 330, 572, 599
FspBI CTAG 1 cut(s) 152
GlaI GCGC 1 cut(s) 1044
GluI GCNGC 4 cut(s) 112, 330, 572, 599
GsaI CCCAGC 1 cut(s) 684
GsuI CTGGAG 2 cut(s) 432, 603
HaeII RGCGCY 1 cut(s) 1046
HaeIII GGCC 2 cut(s) 53, 913
HapII CCGG 3 cut(s) 50, 105, 480
HgaI GACGC 1 cut(s) 1037
HhaI GCGC 1 cut(s) 1045
Hin1II CATG 2 cut(s) 323, 363
Hin6I GCGC 1 cut(s) 1043
HinP1I GCGC 1 cut(s) 1043
HincII GTYRAC 1 cut(s) 1096
HindII GTYRAC 1 cut(s) 1096
HindIII AAGCTT 1 cut(s) 612
HinfI GANTC 5 cut(s) 143, 157, 691, 808, 923
HpaII CCGG 3 cut(s) 50, 105, 480
HphI GGTGA 4 cut(s) 82, 662, 773, 789
Hpy166II GTNNAC 6 cut(s) 130, 461, 938, 1015, 1017, 1096
Hpy188I TCNGA 5 cut(s) 224, 784, 791, 871, 918
Hpy188III TCNNGA 5 cut(s) 178, 233, 320, 384, 707
Hpy8I GTNNAC 6 cut(s) 130, 461, 938, 1015, 1017, 1096
Hpy99I CGWCG 2 cut(s) 224, 884
HpyAV CCTTC 3 cut(s) 226, 269, 437
HpyCH4III ACNGT 6 cut(s) 94, 248, 452, 795, 1012, 1110
HpyCH4V TGCA 3 cut(s) 461, 875, 981
HpyF10VI GCNNNNNNNGC 5 cut(s) 551, 598, 641, 825, 834
HpyF3I CTNAG 6 cut(s) 333, 439, 495, 908, 1038, 1061
Hsp92II CATG 2 cut(s) 323, 363
HspAI GCGC 1 cut(s) 1043
Ksp22I TGATCA 1 cut(s) 316
Kzo9I GATC 5 cut(s) 316, 703, 739, 988, 1089
LmnI GCTCC 4 cut(s) 224, 259, 471, 833
Lsp1109I GCAGC 4 cut(s) 123, 316, 583, 585
LweI GCATC 2 cut(s) 585, 862
MaeI CTAG 1 cut(s) 152
MaeIII GTNAC 5 cut(s) 94, 248, 307, 446, 795
MalI GATC 5 cut(s) 318, 705, 741, 990, 1091
MboI GATC 5 cut(s) 316, 703, 739, 988, 1089
MboII GAAGA 2 cut(s) 511, 911
MfeI CAATTG 2 cut(s) 743, 814
MflI RGATCY 1 cut(s) 988
MhlI GDGCHC 3 cut(s) 76, 463, 830
MmeI TCCRAC 1 cut(s) 202
MroXI GAANNNNTTC 2 cut(s) 643, 948
MseI TTAA 5 cut(s) 198, 255, 354, 513, 717
MslI CAYNNNNRTG 1 cut(s) 453
MspA1I CMGCKG 1 cut(s) 680
MspCI CTTAAG 2 cut(s) 254, 716
MspI CCGG 3 cut(s) 50, 105, 480
MspR9I CCNGG 3 cut(s) 50, 411, 1084
MunI CAATTG 2 cut(s) 743, 814
MvaI CCWGG 2 cut(s) 411, 1084
MvnI CGCG 1 cut(s) 187
MwoI GCNNNNNNNGC 5 cut(s) 551, 598, 641, 825, 834
NciI CCSGG 1 cut(s) 50
NdeII GATC 5 cut(s) 316, 703, 739, 988, 1089
NlaIII CATG 2 cut(s) 323, 363
NlaIV GGNNCC 3 cut(s) 214, 558, 990
NmuCI GTSAC 3 cut(s) 94, 446, 795
PaeR7I CTCGAG 2 cut(s) 75, 1071
PagI TCATGA 1 cut(s) 319
PceI AGGCCT 1 cut(s) 913
PdmI GAANNNNTTC 2 cut(s) 643, 948
PfeI GAWTC 5 cut(s) 143, 157, 691, 808, 923
PfoI TCCNGGA 1 cut(s) 1082
PinAI ACCGGT 1 cut(s) 104
PkrI GCNGC 4 cut(s) 113, 331, 573, 600
Ple19I CGATCG 1 cut(s) 1092
Psp124BI GAGCTC 1 cut(s) 76
Psp6I CCWGG 2 cut(s) 409, 1082
PspFI CCCAGC 1 cut(s) 680
PspGI CCWGG 2 cut(s) 409, 1082
PspN4I GGNNCC 3 cut(s) 214, 558, 990
PspPI GGNCC 1 cut(s) 578
PspXI VCTCGAGB 2 cut(s) 75, 1071
PstNI CAGNNNCTG 2 cut(s) 598, 1031
PsuI RGATCY 1 cut(s) 988
PvuI CGATCG 1 cut(s) 1092
PvuII CAGCTG 1 cut(s) 680
RsaI GTAC 5 cut(s) 37, 302, 858, 937, 1016
RsaNI GTAC 5 cut(s) 36, 301, 857, 936, 1015
RseI CAYNNNNRTG 1 cut(s) 453
SacI GAGCTC 1 cut(s) 76
SaqAI TTAA 5 cut(s) 198, 255, 354, 513, 717
SatI GCNGC 4 cut(s) 112, 330, 572, 599
Sau3AI GATC 5 cut(s) 316, 703, 739, 988, 1089
Sau96I GGNCC 1 cut(s) 578
ScrFI CCNGG 3 cut(s) 50, 411, 1084
SduI GDGCHC 3 cut(s) 76, 463, 830
SfaNI GCATC 2 cut(s) 585, 862
SfcI CTRYAG 1 cut(s) 832
Sfr274I CTCGAG 2 cut(s) 75, 1071
SinI GGWCC 1 cut(s) 578
SlaI CTCGAG 2 cut(s) 75, 1071
SmiMI CAYNNNNRTG 1 cut(s) 453
SmlI CTYRAG 5 cut(s) 75, 254, 716, 803, 1071
SmoI CTYRAG 5 cut(s) 75, 254, 716, 803, 1071
SseBI AGGCCT 1 cut(s) 913
SsiI CCGC 2 cut(s) 185, 554
SspMI CTAG 1 cut(s) 152
SstI GAGCTC 1 cut(s) 76
StuI AGGCCT 1 cut(s) 913
StyD4I CCNGG 3 cut(s) 48, 409, 1082
TaaI ACNGT 6 cut(s) 94, 248, 452, 795, 1012, 1110
TaqI TCGA 6 cut(s) 23, 76, 637, 879, 897, 1072
TatI WGTACW 1 cut(s) 1014
TfiI GAWTC 5 cut(s) 143, 157, 691, 808, 923
Tru1I TTAA 5 cut(s) 198, 255, 354, 513, 717
Tru9I TTAA 5 cut(s) 198, 255, 354, 513, 717
TscAI CASTG 1 cut(s) 468
TseFI GTSAC 3 cut(s) 94, 446, 795
TseI GCWGC 4 cut(s) 111, 329, 571, 598
Tsp45I GTSAC 3 cut(s) 94, 446, 795
TspDTI ATGAA 4 cut(s) 336, 348, 516, 642
TspGWI ACGGA 1 cut(s) 975
TspRI CASTG 1 cut(s) 468
Vha464I CTTAAG 2 cut(s) 254, 716
VneI GTGCAC 1 cut(s) 459
VpaK11BI GGWCC 1 cut(s) 578
XapI RAATTY 5 cut(s) 173, 200, 387, 394, 785
XhoI CTCGAG 2 cut(s) 75, 1071
XmnI GAANNNNTTC 2 cut(s) 643, 948
XspI CTAG 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.