Rh6CG459000

Protein of unknown function (DUF1350)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0013928
Physical Location & Seq
Forward (+)
63369746 .. 63372878
3133 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG459000.1

Sequence Viewer

Length: 1194 bp
ATGGAAGTTACCACTTCGTCTTCCTCCGCCAAATTTGGATGCGCATACGACTTCCCTCAATTCCGAGTCAACCGTCATGGCCATAGTTGCTTCTTCCGATTTCCATGGAAGAGCAGAGCAGTTGTTGCAGTGAGCTCAACTTCATCCACTACCAACACCACCACTAGTGTCAGTAATGGGTATGTGGGCCCAGGCATCCGAAACTACACCCGGAGCGGTTCTTGCTTGCTGATCCCTCCGCCGGCCGGCAAGAAGCCCCGGGCTATCATCAAGTTTCTGGGCGGTGCCTTCATCGGGGCTGTTCCTGAAGTCACTTACAGCTACTTGACTGAGCTATTGGCCAAGGAGGGGTTTCTGGTTATTTCAGTGCCGTATAATGTGACCTTTGATCATGTCCAAGCTGCTCACCAAGTCTATGAGAGGTTCAACTCTTGCTTGGACACCATCTTAGCCTCTGGATTGCCCAATGTTGATCTATCACCTGCCCAACTAGCTGAGTTGCCAGTGTTTTCTGTTGGCCATAGCAATGGTGCACTACTCCAAGTGCTCGCTGGGAGCTATTTCTCCGATAAAATCCCAAAGGCTAATGCCATAATCGCATACAACAATAGACCAGCAACAGAGGCTGTCCCTTACTTTGAGCAGCTTGGACCGTTAGTTAATCAGATGGTCCCTATTGTAGAAGCATCTCCCGTTCCTTCCATGGCTAGGACTGCCTCTGGAGATGCATGGAAGGCTCTAGTTGATGCAGCTGGAGCAGTTCTACCAGACAATGAAAAGGAAACTCTAAATTCACTCACTAAGTTTGTTGATCAGTTGCCGTTGGTGCTGAATGAGGTAACCCAAGGGACATCAGAGTTTAAGCCAACACCCTCTGAAAATCGTGAATTTTTTAAGAGCTCATACAATGTGAAACACACTCTACTGGTGAAGTTCAATTTTGACGGAATTGATGAGACAGATACTCTCGAGGACACATTGAAGCCTCGTGTGGAAGCTATTGGCGGGACACTAGAAAAGGTCGAGATAAGTGGTAACCATATCACACCATGCGTCCAGGAGCCCAAGTGGCAAGTAGGTGCCTTGTACACTCCTGCAGATGCTATTGCTCAGAGTCTTAAAACTCTTTCGCTAAATGATGTTAGAGTCCTATCAAGAACTATAATCGACTGGTTTCGAGGTTTTGAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1350

Protein Analysis

397

Amino Acids

43.23

Weight (kDa)

5.87

Isoelectric Point (pI)

28.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1350 PF07082 72 - 392 9.8e-50 Protein of unknown function (DUF1350)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013928)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G43540 AT3G43540
fragaria_vesca FvH4_2g37050 FvH4_2g37050
malus_domestica MD08G1047000.v1.1
pyrus_communis pycom08g03940
rosa_chinensis RchiOBHm_Chr6g0304731
rosa_laevigata RLG00000010955
rosa_multiflora Rmu_co8515209.1_g000001
rosa_roxburghii Rroxscaffold_7G00163550
rosa_rugosa Rorug06G0333900 Rorug06G0334000
rosa_samantha Rh6AG445900 Rh6BG455800 Rh6CG459000 Rh6DG446000
rosa_wichuraiana Rw6G038790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 490
Acc16I TGCGCA 1 cut(s) 43
Acc36I ACCTGC 1 cut(s) 490
AccB1I GGYRCC 2 cut(s) 284, 1079
AccBSI CCGCTC 1 cut(s) 216
AciI CCGC 5 cut(s) 27, 216, 239, 282, 1005
AclWI GGATC 1 cut(s) 226
AcoI YGGCCR 4 cut(s) 79, 243, 339, 517
AcsI RAATTY 3 cut(s) 32, 790, 887
AcuI CTGAAG 1 cut(s) 327
AfaI GTAC 1 cut(s) 1088
AfiI CCNNNNNNNGG 5 cut(s) 241, 245, 294, 348, 708
AgsI TTSAA 4 cut(s) 427, 937, 982, 1187
AhlI ACTAGT 1 cut(s) 164
AjnI CCWGG 2 cut(s) 190, 1056
Alw21I GWGCWC 4 cut(s) 137, 535, 549, 902
Alw26I GTCTC 1 cut(s) 950
Alw44I GTGCAC 1 cut(s) 531
AlwI GGATC 1 cut(s) 226
AlwNI CAGNNNCTG 1 cut(s) 626
Ama87I CYCGRG 2 cut(s) 258, 968
AoxI GGCC 5 cut(s) 79, 187, 243, 339, 517
ApaI GGGCCC 1 cut(s) 191
ApaLI GTGCAC 1 cut(s) 531
ApeKI GCWGC 3 cut(s) 401, 643, 749
ApoI RAATTY 3 cut(s) 32, 790, 887
AspLEI GCGC 1 cut(s) 44
AspS9I GGNCC 4 cut(s) 187, 188, 650, 670
AsuC2I CCSGG 3 cut(s) 211, 259, 260
AsuHPI GGTGA 3 cut(s) 398, 471, 940
AvaI CYCGRG 2 cut(s) 258, 968
AvaII GGWCC 2 cut(s) 650, 670
BaeGI GKGCMC 2 cut(s) 191, 535
BalI TGGCCA 3 cut(s) 81, 341, 519
BanI GGYRCC 2 cut(s) 284, 1079
BanII GRGCYC 4 cut(s) 137, 191, 902, 1065
BauI CACGAG 1 cut(s) 987
BbsI GAAGAC 1 cut(s) 12
Bbv12I GWGCWC 4 cut(s) 137, 535, 549, 902
BbvI GCAGC 3 cut(s) 388, 655, 761
BccI CCATC 2 cut(s) 452, 661
BceAI ACGGC 2 cut(s) 355, 805
BciT130I CCWGG 2 cut(s) 192, 1058
BclI TGATCA 2 cut(s) 388, 811
BcnI CCSGG 3 cut(s) 211, 259, 260
BcoDI GTCTC 1 cut(s) 950
BcuI ACTAGT 1 cut(s) 164
BfaI CTAG 5 cut(s) 165, 491, 708, 740, 1013
BfmI CTRYAG 1 cut(s) 1095
BfuAI ACCTGC 1 cut(s) 490
BglI GCCNNNNNGGC 1 cut(s) 1069
BisI GCNGC 3 cut(s) 402, 644, 750
BlsI GCNGC 3 cut(s) 403, 645, 751
Bme1390I CCNGG 5 cut(s) 192, 211, 259, 260, 1058
Bme18I GGWCC 2 cut(s) 650, 670
BmeT110I CYCGRG 2 cut(s) 258, 968
BmgT120I GGNCC 4 cut(s) 187, 188, 650, 670
BmiI GGNNCC 5 cut(s) 189, 286, 672, 1062, 1081
BmrFI CCNGG 5 cut(s) 192, 211, 259, 260, 1058
BmsI GCATC 6 cut(s) 29, 204, 695, 715, 736, 1090
BpiI GAAGAC 1 cut(s) 12
BpmI CTGGAG 2 cut(s) 741, 774
BpuMI CCSGG 3 cut(s) 211, 259, 260
BsaJI CCNNGG 7 cut(s) 104, 190, 257, 258, 342, 702, 844
BsaXI ACNNNNNCTCC 2 cut(s) 1052, 1082
Bsc4I CCNNNNNNNGG 5 cut(s) 241, 245, 294, 348, 708
Bse118I RCCGGY 2 cut(s) 241, 245
Bse1I ACTGG 3 cut(s) 503, 930, 1175
Bse3DI GCAATG 1 cut(s) 532
BseBI CCWGG 2 cut(s) 192, 1058
BseDI CCNNGG 7 cut(s) 104, 190, 257, 258, 342, 702, 844
BseGI GGATG 3 cut(s) 44, 143, 195
BseLI CCNNNNNNNGG 5 cut(s) 241, 245, 294, 348, 708
BseMI GCAATG 1 cut(s) 532
BseMII CTCAG 3 cut(s) 321, 486, 1124
BseNI ACTGG 3 cut(s) 503, 930, 1175
BseSI GKGCMC 2 cut(s) 191, 535
BseX3I CGGCCG 1 cut(s) 243
BseXI GCAGC 3 cut(s) 388, 655, 761
BseYI CCCAGC 1 cut(s) 551
Bsh1285I CGRYCG 1 cut(s) 246
BshFI GGCC 5 cut(s) 81, 189, 245, 341, 519
BshNI GGYRCC 2 cut(s) 284, 1079
BsiEI CGRYCG 1 cut(s) 246
BsiHKAI GWGCWC 4 cut(s) 137, 535, 549, 902
BsiHKCI CYCGRG 2 cut(s) 258, 968
BsiSI CCGG 4 cut(s) 211, 242, 246, 259
BslFI GGGAC 4 cut(s) 614, 656, 862, 1021
BslI CCNNNNNNNGG 5 cut(s) 241, 245, 294, 348, 708
BsmAI GTCTC 1 cut(s) 950
BsmFI GGGAC 4 cut(s) 614, 656, 862, 1021
BsnI GGCC 5 cut(s) 81, 189, 245, 341, 519
BsoBI CYCGRG 2 cut(s) 258, 968
Bsp120I GGGCCC 1 cut(s) 187
Bsp1286I GDGCHC 6 cut(s) 137, 191, 535, 549, 902, 1065
Bsp1407I TGTACA 1 cut(s) 1086
Bsp143I GATC 4 cut(s) 231, 388, 472, 811
Bsp19I CCATGG 2 cut(s) 104, 702
BspACI CCGC 5 cut(s) 27, 216, 239, 282, 1005
BspANI GGCC 5 cut(s) 81, 189, 245, 341, 519
BspCNI CTCAG 3 cut(s) 322, 487, 1123
BspLI GGNNCC 5 cut(s) 189, 286, 672, 1062, 1081
BspMAI CTGCAG 1 cut(s) 1099
BspMI ACCTGC 1 cut(s) 490
BspPI GGATC 1 cut(s) 226
BspQI GCTCTTC 1 cut(s) 104
BspT107I GGYRCC 2 cut(s) 284, 1079
BsrBI CCGCTC 1 cut(s) 216
BsrDI GCAATG 1 cut(s) 532
BsrFI RCCGGY 2 cut(s) 241, 245
BsrGI TGTACA 1 cut(s) 1086
BsrI ACTGG 3 cut(s) 503, 930, 1175
BssAI RCCGGY 2 cut(s) 241, 245
BssECI CCNNGG 7 cut(s) 104, 190, 257, 258, 342, 702, 844
BssMI GATC 4 cut(s) 231, 388, 472, 811
BssSI CACGAG 1 cut(s) 987
BssT1I CCWWGG 4 cut(s) 104, 342, 702, 844
Bst2BI CACGAG 1 cut(s) 987
Bst2UI CCWGG 2 cut(s) 192, 1058
Bst4CI ACNGT 2 cut(s) 74, 654
Bst6I CTCTTC 1 cut(s) 104
BstAPI GCANNNNNTGC 1 cut(s) 125
BstAUI TGTACA 1 cut(s) 1086
BstC8I GCNNGC 4 cut(s) 227, 243, 247, 549
BstDEI CTNAG 5 cut(s) 330, 448, 495, 801, 1110
BstDSI CCRYGG 2 cut(s) 104, 702
BstEII GGTNACC 2 cut(s) 838, 1034
BstF5I GGATG 3 cut(s) 44, 143, 195
BstHHI GCGC 1 cut(s) 44
BstKTI GATC 4 cut(s) 234, 391, 475, 814
BstMAI GTCTC 1 cut(s) 950
BstMBI GATC 4 cut(s) 231, 388, 472, 811
BstMCI CGRYCG 1 cut(s) 246
BstNI CCWGG 2 cut(s) 192, 1058
BstPI GGTNACC 2 cut(s) 838, 1034
BstSCI CCNGG 5 cut(s) 190, 209, 257, 258, 1056
BstSFI CTRYAG 1 cut(s) 1095
BstSLI GKGCMC 2 cut(s) 191, 535
BstV1I GCAGC 3 cut(s) 388, 655, 761
BstV2I GAAGAC 1 cut(s) 12
BstXI CCANNNNNNTGG 1 cut(s) 527
BstZI CGGCCG 1 cut(s) 243
BsuRI GGCC 5 cut(s) 81, 189, 245, 341, 519
BtgI CCRYGG 2 cut(s) 104, 702
BtsCI GGATG 3 cut(s) 44, 143, 195
BtsI GCAGTG 1 cut(s) 135
BtsIMutI CAGTG 3 cut(s) 135, 372, 510
BveI ACCTGC 1 cut(s) 490
Cac8I GCNNGC 4 cut(s) 227, 243, 247, 549
CaiI CAGNNNCTG 1 cut(s) 626
CfoI GCGC 1 cut(s) 44
Cfr10I RCCGGY 2 cut(s) 241, 245
Cfr13I GGNCC 4 cut(s) 187, 188, 650, 670
Cfr9I CCCGGG 1 cut(s) 258
CseI GACGC 1 cut(s) 1042
Csp6I GTAC 1 cut(s) 1087
CviAII CATG 6 cut(s) 77, 105, 392, 703, 729, 1050
CviQI GTAC 1 cut(s) 1087
DdeI CTNAG 5 cut(s) 330, 448, 495, 801, 1110
DpnI GATC 4 cut(s) 233, 390, 474, 813
DpnII GATC 4 cut(s) 231, 388, 472, 811
EaeI YGGCCR 4 cut(s) 79, 243, 339, 517
EagI CGGCCG 1 cut(s) 243
Eam1104I CTCTTC 1 cut(s) 104
EarI CTCTTC 1 cut(s) 104
EciI GGCGGA 2 cut(s) 16, 228
Ecl136II GAGCTC 2 cut(s) 135, 900
EclXI CGGCCG 1 cut(s) 243
Eco130I CCWWGG 4 cut(s) 104, 342, 702, 844
Eco24I GRGCYC 4 cut(s) 137, 191, 902, 1065
Eco47I GGWCC 2 cut(s) 650, 670
Eco52I CGGCCG 1 cut(s) 243
Eco53kI GAGCTC 2 cut(s) 135, 900
Eco57I CTGAAG 1 cut(s) 327
Eco88I CYCGRG 2 cut(s) 258, 968
Eco91I GGTNACC 2 cut(s) 838, 1034
EcoICRI GAGCTC 2 cut(s) 135, 900
EcoO65I GGTNACC 2 cut(s) 838, 1034
EcoRII CCWGG 2 cut(s) 190, 1056
EcoT14I CCWWGG 4 cut(s) 104, 342, 702, 844
EcoT22I ATGCAT 1 cut(s) 730
EcoT38I GRGCYC 4 cut(s) 137, 191, 902, 1065
ErhI CCWWGG 4 cut(s) 104, 342, 702, 844
FaeI CATG 6 cut(s) 80, 108, 395, 706, 732, 1053
FaqI GGGAC 4 cut(s) 614, 656, 862, 1021
FatI CATG 6 cut(s) 76, 104, 391, 702, 728, 1049
FauI CCCGC 1 cut(s) 998
FbaI TGATCA 2 cut(s) 388, 811
Fnu4HI GCNGC 3 cut(s) 402, 644, 750
FokI GGATG 3 cut(s) 51, 130, 182
FriOI GRGCYC 4 cut(s) 137, 191, 902, 1065
Fsp4HI GCNGC 3 cut(s) 402, 644, 750
FspAI RTGCGCAY 1 cut(s) 43
FspBI CTAG 5 cut(s) 165, 491, 708, 740, 1013
FspI TGCGCA 1 cut(s) 43
GlaI GCGC 1 cut(s) 43
GluI GCNGC 3 cut(s) 402, 644, 750
GsaI CCCAGC 1 cut(s) 555
GsuI CTGGAG 2 cut(s) 741, 774
HaeIII GGCC 5 cut(s) 81, 189, 245, 341, 519
HapII CCGG 4 cut(s) 211, 242, 246, 259
HgaI GACGC 1 cut(s) 1042
HhaI GCGC 1 cut(s) 44
Hin1II CATG 6 cut(s) 80, 108, 395, 706, 732, 1053
Hin6I GCGC 1 cut(s) 42
HinP1I GCGC 1 cut(s) 42
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HinfI GANTC 3 cut(s) 66, 1114, 1146
HpaII CCGG 4 cut(s) 211, 242, 246, 259
HphI GGTGA 3 cut(s) 398, 471, 940
Hpy166II GTNNAC 3 cut(s) 70, 533, 1089
Hpy188I TCNGA 8 cut(s) 65, 98, 200, 568, 666, 856, 877, 1113
Hpy188III TCNNGA 7 cut(s) 305, 456, 720, 884, 968, 1024, 1155
Hpy8I GTNNAC 3 cut(s) 70, 533, 1089
HpyAV CCTTC 3 cut(s) 298, 708, 727
HpyCH4III ACNGT 2 cut(s) 74, 654
HpyCH4V TGCA 5 cut(s) 128, 533, 728, 749, 1097
HpyF3I CTNAG 5 cut(s) 330, 448, 495, 801, 1110
Hsp92II CATG 6 cut(s) 80, 108, 395, 706, 732, 1053
HspAI GCGC 1 cut(s) 42
KroI GCCGGC 2 cut(s) 241, 245
KroNI GCCGGC 2 cut(s) 243, 247
Ksp22I TGATCA 2 cut(s) 388, 811
Kzo9I GATC 4 cut(s) 231, 388, 472, 811
LguI GCTCTTC 1 cut(s) 104
LmnI GCTCC 4 cut(s) 213, 555, 755, 1060
Lsp1109I GCAGC 3 cut(s) 388, 655, 761
LweI GCATC 6 cut(s) 29, 204, 695, 715, 736, 1090
MaeI CTAG 5 cut(s) 165, 491, 708, 740, 1013
MaeIII GTNAC 5 cut(s) 7, 310, 379, 838, 1034
MalI GATC 4 cut(s) 233, 390, 474, 813
MbiI CCGCTC 1 cut(s) 216
MboI GATC 4 cut(s) 231, 388, 472, 811
MboII GAAGA 3 cut(s) 12, 85, 121
MhlI GDGCHC 6 cut(s) 137, 191, 535, 549, 902, 1065
MlsI TGGCCA 3 cut(s) 81, 341, 519
MluCI AATT 6 cut(s) 32, 59, 790, 887, 937, 948
MluNI TGGCCA 3 cut(s) 81, 341, 519
MlyI GAGTC 3 cut(s) 75, 1123, 1155
Mox20I TGGCCA 3 cut(s) 81, 341, 519
Mph1103I ATGCAT 1 cut(s) 730
MroNI GCCGGC 2 cut(s) 241, 245
MscI TGGCCA 3 cut(s) 81, 341, 519
MseI TTAA 4 cut(s) 660, 861, 894, 1119
MslI CAYNNNNRTG 1 cut(s) 525
Msp20I TGGCCA 3 cut(s) 81, 341, 519
MspA1I CMGCKG 1 cut(s) 752
MspI CCGG 4 cut(s) 211, 242, 246, 259
MspR9I CCNGG 5 cut(s) 192, 211, 259, 260, 1058
MvaI CCWGG 2 cut(s) 192, 1058
NaeI GCCGGC 2 cut(s) 243, 247
NciI CCSGG 3 cut(s) 211, 259, 260
NcoI CCATGG 2 cut(s) 104, 702
NdeII GATC 4 cut(s) 231, 388, 472, 811
NgoMIV GCCGGC 2 cut(s) 241, 245
NlaIII CATG 6 cut(s) 80, 108, 395, 706, 732, 1053
NlaIV GGNNCC 5 cut(s) 189, 286, 672, 1062, 1081
NmuCI GTSAC 2 cut(s) 310, 379
NsbI TGCGCA 1 cut(s) 43
NsiI ATGCAT 1 cut(s) 730
PaeR7I CTCGAG 1 cut(s) 968
PaqCI CACCTGC 1 cut(s) 490
PciSI GCTCTTC 1 cut(s) 104
PdiI GCCGGC 2 cut(s) 243, 247
PfoI TCCNGGA 1 cut(s) 1056
PkrI GCNGC 3 cut(s) 403, 645, 751
PleI GAGTC 3 cut(s) 74, 1122, 1154
PpsI GAGTC 3 cut(s) 74, 1122, 1154
Psp124BI GAGCTC 2 cut(s) 137, 902
Psp6I CCWGG 2 cut(s) 190, 1056
PspEI GGTNACC 2 cut(s) 838, 1034
PspFI CCCAGC 1 cut(s) 551
PspGI CCWGG 2 cut(s) 190, 1056
PspN4I GGNNCC 5 cut(s) 189, 286, 672, 1062, 1081
PspOMI GGGCCC 1 cut(s) 187
PspPI GGNCC 4 cut(s) 187, 188, 650, 670
PstI CTGCAG 1 cut(s) 1099
PstNI CAGNNNCTG 1 cut(s) 626
PvuII CAGCTG 1 cut(s) 752
RsaI GTAC 1 cut(s) 1088
RsaNI GTAC 1 cut(s) 1087
RseI CAYNNNNRTG 1 cut(s) 525
SacI GAGCTC 2 cut(s) 137, 902
SapI GCTCTTC 1 cut(s) 104
SaqAI TTAA 4 cut(s) 660, 861, 894, 1119
SatI GCNGC 3 cut(s) 402, 644, 750
Sau3AI GATC 4 cut(s) 231, 388, 472, 811
Sau96I GGNCC 4 cut(s) 187, 188, 650, 670
SchI GAGTC 3 cut(s) 75, 1123, 1155
ScrFI CCNGG 5 cut(s) 192, 211, 259, 260, 1058
SduI GDGCHC 6 cut(s) 137, 191, 535, 549, 902, 1065
SfaNI GCATC 6 cut(s) 29, 204, 695, 715, 736, 1090
SfcI CTRYAG 1 cut(s) 1095
Sfr274I CTCGAG 1 cut(s) 968
SinI GGWCC 2 cut(s) 650, 670
SlaI CTCGAG 1 cut(s) 968
SmaI CCCGGG 1 cut(s) 260
SmiMI CAYNNNNRTG 1 cut(s) 525
SmlI CTYRAG 1 cut(s) 968
SmoI CTYRAG 1 cut(s) 968
SpeI ACTAGT 1 cut(s) 164
Sse9I AATT 6 cut(s) 32, 59, 790, 887, 937, 948
SsiI CCGC 5 cut(s) 27, 216, 239, 282, 1005
SspMI CTAG 5 cut(s) 165, 491, 708, 740, 1013
SstI GAGCTC 2 cut(s) 137, 902
StyD4I CCNGG 5 cut(s) 190, 209, 257, 258, 1056
StyI CCWWGG 4 cut(s) 104, 342, 702, 844
TaaI ACNGT 2 cut(s) 74, 654
TaqI TCGA 4 cut(s) 969, 1023, 1167, 1177
TasI AATT 6 cut(s) 32, 59, 790, 887, 937, 948
TatI WGTACW 1 cut(s) 1086
Tru1I TTAA 4 cut(s) 660, 861, 894, 1119
Tru9I TTAA 4 cut(s) 660, 861, 894, 1119
TscAI CASTG 3 cut(s) 135, 372, 510
TseFI GTSAC 2 cut(s) 310, 379
TseI GCWGC 3 cut(s) 401, 643, 749
Tsp45I GTSAC 2 cut(s) 310, 379
TspDTI ATGAA 3 cut(s) 132, 280, 789
TspGWI ACGGA 1 cut(s) 960
TspMI CCCGGG 1 cut(s) 258
TspRI CASTG 3 cut(s) 135, 372, 510
VneI GTGCAC 1 cut(s) 531
VpaK11BI GGWCC 2 cut(s) 650, 670
XapI RAATTY 3 cut(s) 32, 790, 887
XcmI CCANNNNNNNNNTGG 1 cut(s) 548
XhoI CTCGAG 1 cut(s) 968
XmaI CCCGGG 1 cut(s) 258
XspI CTAG 5 cut(s) 165, 491, 708, 740, 1013
Zsp2I ATGCAT 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.