Rh6AG445900

Protein of unknown function (DUF1350)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0013928
Physical Location & Seq
Forward (+)
63291244 .. 63294621
3378 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG445900.1

Sequence Viewer

Length: 864 bp
ATGGAAGTTACCACTTCGTCTTCCTCCGCCAAATTTGGATGCGCATACGACTTCCCTCAATTCCAAATCAAACGTCATGGCCATAGTTGCTTCTTCCGATTTCCATGGAAGAGCAGAGCAGTTGTTGCAGTGAGCTCAACTTCATCCACTACCAACACCACCACTAGTGTCAGTAATGGGTATGTGGGCCCAGGCATCCGAAACTACACCCGGAGCGGTTCTTGCTTGCTGATCCCTCCGCCGGCCGGCAAGAAGCCCCGGGCTATCATCAAGTTTCTGGGCGGTGCCTTCATCGGAGCTGTTCCTGAAGTCACTTACAGCTACTTGACTGAGCTATTGGCCAAGGAGGGGTTTCTGGTTATTTCAGTGCCGTATAATGTGACCTTTGATCATGTCCAAGCTGCTCACCAAGTCTATGAGAGGTTCAACTCTTGCTTGGACACCGTCTTAGCCTCTGGATTGCCCGATGTTGATCTATCACCTGCCCAACTAGCTGAGTTGCCAGTGTTTTCTGTTGGCCATAGCAATGGTGCACTACTCCAAGTGCTCGCTGGGAGCTATTTCTCCGATAAAATCCCAAAGGCTAATGCCATAATCGCATACAACAATAGACCAGCAACAGAGGCTGTCCCTTACTTTGAGCAGCTTGGACCGTTGGTTAATCAGATGGTCCCTATTGTAGAAGCATCTCCTGTTCCTTCCATGGCAAGGACTGCCTCAGGTAGCTTCCTGATCGACATGTGTATACCATTACAGTATAAAGTTTCGTTTCTTTTTTTTTTTTTTTTCTGTTCTAGTTATGGGTTGCACATTGGCTTACTTATAGGAGTAGATCCATGCCTTGCTTTCTGTTTACTGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1350

Protein Analysis

287

Amino Acids

31.04

Weight (kDa)

8.08

Isoelectric Point (pI)

36.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1350 PF07082 72 - 233 1.7e-27 Protein of unknown function (DUF1350)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013928)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G43540 AT3G43540
fragaria_vesca FvH4_2g37050 FvH4_2g37050
malus_domestica MD08G1047000.v1.1
pyrus_communis pycom08g03940
rosa_chinensis RchiOBHm_Chr6g0304731
rosa_laevigata RLG00000010955
rosa_multiflora Rmu_co8515209.1_g000001
rosa_roxburghii Rroxscaffold_7G00163550
rosa_rugosa Rorug06G0333900 Rorug06G0334000
rosa_samantha Rh6AG445900 Rh6BG455800 Rh6CG459000 Rh6DG446000
rosa_wichuraiana Rw6G038790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 490
Acc16I TGCGCA 1 cut(s) 43
Acc36I ACCTGC 1 cut(s) 490
AccB1I GGYRCC 1 cut(s) 284
AccBSI CCGCTC 1 cut(s) 216
AccI GTMKAC 1 cut(s) 745
AciI CCGC 4 cut(s) 27, 216, 239, 282
AclWI GGATC 2 cut(s) 226, 827
AcoI YGGCCR 4 cut(s) 79, 243, 339, 517
AcsI RAATTY 1 cut(s) 32
AcuI CTGAAG 1 cut(s) 327
AfiI CCNNNNNNNGG 4 cut(s) 241, 245, 348, 708
AflIII ACRYGT 1 cut(s) 738
AgsI TTSAA 1 cut(s) 427
AhlI ACTAGT 1 cut(s) 164
AjnI CCWGG 1 cut(s) 190
Alw21I GWGCWC 3 cut(s) 137, 535, 549
Alw44I GTGCAC 1 cut(s) 531
AlwI GGATC 2 cut(s) 226, 827
AlwNI CAGNNNCTG 1 cut(s) 626
Ama87I CYCGRG 1 cut(s) 258
AoxI GGCC 5 cut(s) 79, 187, 243, 339, 517
ApaI GGGCCC 1 cut(s) 191
ApaLI GTGCAC 1 cut(s) 531
ApeKI GCWGC 2 cut(s) 401, 643
ApoI RAATTY 1 cut(s) 32
ArsI GACNNNNNNTTYG 2 cut(s) 58, 90
AspLEI GCGC 1 cut(s) 44
AspS9I GGNCC 4 cut(s) 187, 188, 650, 670
AsuC2I CCSGG 3 cut(s) 211, 259, 260
AsuHPI GGTGA 2 cut(s) 398, 471
AvaI CYCGRG 1 cut(s) 258
AvaII GGWCC 2 cut(s) 650, 670
AxyI CCTNAGG 1 cut(s) 718
BaeGI GKGCMC 2 cut(s) 191, 535
BalI TGGCCA 3 cut(s) 81, 341, 519
BanI GGYRCC 1 cut(s) 284
BanII GRGCYC 2 cut(s) 137, 191
BbsI GAAGAC 1 cut(s) 12
Bbv12I GWGCWC 3 cut(s) 137, 535, 549
BbvI GCAGC 2 cut(s) 388, 655
BccI CCATC 1 cut(s) 661
BceAI ACGGC 1 cut(s) 355
BciT130I CCWGG 1 cut(s) 192
BclI TGATCA 1 cut(s) 388
BcnI CCSGG 3 cut(s) 211, 259, 260
BcuI ACTAGT 1 cut(s) 164
BfaI CTAG 4 cut(s) 165, 491, 795, 862
BfuAI ACCTGC 1 cut(s) 490
BisI GCNGC 2 cut(s) 402, 644
BlsI GCNGC 2 cut(s) 403, 645
Bme1390I CCNGG 4 cut(s) 192, 211, 259, 260
Bme18I GGWCC 2 cut(s) 650, 670
BmeT110I CYCGRG 1 cut(s) 258
BmgT120I GGNCC 4 cut(s) 187, 188, 650, 670
BmiI GGNNCC 3 cut(s) 189, 286, 672
BmrFI CCNGG 4 cut(s) 192, 211, 259, 260
BmsI GCATC 3 cut(s) 29, 204, 695
BpiI GAAGAC 1 cut(s) 12
BpuMI CCSGG 3 cut(s) 211, 259, 260
BsaBI GATNNNNATC 1 cut(s) 471
BsaJI CCNNGG 6 cut(s) 104, 190, 257, 258, 342, 702
Bsc4I CCNNNNNNNGG 4 cut(s) 241, 245, 348, 708
Bse118I RCCGGY 2 cut(s) 241, 245
Bse1I ACTGG 1 cut(s) 503
Bse21I CCTNAGG 1 cut(s) 718
Bse3DI GCAATG 1 cut(s) 532
Bse8I GATNNNNATC 1 cut(s) 471
BseBI CCWGG 1 cut(s) 192
BseDI CCNNGG 6 cut(s) 104, 190, 257, 258, 342, 702
BseGI GGATG 3 cut(s) 44, 143, 195
BseJI GATNNNNATC 1 cut(s) 471
BseLI CCNNNNNNNGG 4 cut(s) 241, 245, 348, 708
BseMI GCAATG 1 cut(s) 532
BseMII CTCAG 4 cut(s) 321, 486, 732, 848
BseNI ACTGG 1 cut(s) 503
BseSI GKGCMC 2 cut(s) 191, 535
BseX3I CGGCCG 1 cut(s) 243
BseXI GCAGC 2 cut(s) 388, 655
BseYI CCCAGC 1 cut(s) 551
Bsh1285I CGRYCG 1 cut(s) 246
BshFI GGCC 5 cut(s) 81, 189, 245, 341, 519
BshNI GGYRCC 1 cut(s) 284
BsiEI CGRYCG 1 cut(s) 246
BsiHKAI GWGCWC 3 cut(s) 137, 535, 549
BsiHKCI CYCGRG 1 cut(s) 258
BsiSI CCGG 4 cut(s) 211, 242, 246, 259
BslFI GGGAC 2 cut(s) 614, 656
BslI CCNNNNNNNGG 4 cut(s) 241, 245, 348, 708
BsmFI GGGAC 2 cut(s) 614, 656
BsnI GGCC 5 cut(s) 81, 189, 245, 341, 519
BsoBI CYCGRG 1 cut(s) 258
Bsp120I GGGCCC 1 cut(s) 187
Bsp1286I GDGCHC 4 cut(s) 137, 191, 535, 549
Bsp143I GATC 5 cut(s) 231, 388, 472, 732, 832
Bsp19I CCATGG 2 cut(s) 104, 702
BspACI CCGC 4 cut(s) 27, 216, 239, 282
BspANI GGCC 5 cut(s) 81, 189, 245, 341, 519
BspCNI CTCAG 4 cut(s) 322, 487, 731, 849
BspLI GGNNCC 3 cut(s) 189, 286, 672
BspMI ACCTGC 1 cut(s) 490
BspPI GGATC 2 cut(s) 226, 827
BspQI GCTCTTC 1 cut(s) 104
BspT107I GGYRCC 1 cut(s) 284
BsrBI CCGCTC 1 cut(s) 216
BsrDI GCAATG 1 cut(s) 532
BsrFI RCCGGY 2 cut(s) 241, 245
BsrI ACTGG 1 cut(s) 503
BssAI RCCGGY 2 cut(s) 241, 245
BssECI CCNNGG 6 cut(s) 104, 190, 257, 258, 342, 702
BssMI GATC 5 cut(s) 231, 388, 472, 732, 832
BssNAI GTATAC 1 cut(s) 746
BssT1I CCWWGG 3 cut(s) 104, 342, 702
Bst1107I GTATAC 1 cut(s) 746
Bst2UI CCWGG 1 cut(s) 192
Bst4CI ACNGT 3 cut(s) 445, 654, 756
Bst6I CTCTTC 1 cut(s) 104
BstAPI GCANNNNNTGC 2 cut(s) 125, 713
BstC8I GCNNGC 4 cut(s) 227, 243, 247, 549
BstDEI CTNAG 5 cut(s) 330, 448, 495, 718, 857
BstDSI CCRYGG 2 cut(s) 104, 702
BstF5I GGATG 3 cut(s) 44, 143, 195
BstHHI GCGC 1 cut(s) 44
BstKTI GATC 5 cut(s) 234, 391, 475, 735, 835
BstMBI GATC 5 cut(s) 231, 388, 472, 732, 832
BstMCI CGRYCG 1 cut(s) 246
BstMWI GCNNNNNNNGC 7 cut(s) 87, 125, 222, 491, 596, 623, 713
BstNI CCWGG 1 cut(s) 192
BstNSI RCATGY 1 cut(s) 742
BstSCI CCNGG 4 cut(s) 190, 209, 257, 258
BstSLI GKGCMC 2 cut(s) 191, 535
BstV1I GCAGC 2 cut(s) 388, 655
BstV2I GAAGAC 1 cut(s) 12
BstX2I RGATCY 1 cut(s) 832
BstXI CCANNNNNNTGG 1 cut(s) 527
BstYI RGATCY 1 cut(s) 832
BstZ17I GTATAC 1 cut(s) 746
BstZI CGGCCG 1 cut(s) 243
Bsu36I CCTNAGG 1 cut(s) 718
BsuRI GGCC 5 cut(s) 81, 189, 245, 341, 519
BtgI CCRYGG 2 cut(s) 104, 702
BtsCI GGATG 3 cut(s) 44, 143, 195
BtsI GCAGTG 1 cut(s) 135
BtsIMutI CAGTG 3 cut(s) 135, 372, 510
BveI ACCTGC 1 cut(s) 490
Cac8I GCNNGC 4 cut(s) 227, 243, 247, 549
CaiI CAGNNNCTG 1 cut(s) 626
CfoI GCGC 1 cut(s) 44
Cfr10I RCCGGY 2 cut(s) 241, 245
Cfr13I GGNCC 4 cut(s) 187, 188, 650, 670
Cfr9I CCCGGG 1 cut(s) 258
CviAII CATG 6 cut(s) 77, 105, 392, 703, 739, 837
DdeI CTNAG 5 cut(s) 330, 448, 495, 718, 857
DpnI GATC 5 cut(s) 233, 390, 474, 734, 834
DpnII GATC 5 cut(s) 231, 388, 472, 732, 832
EaeI YGGCCR 4 cut(s) 79, 243, 339, 517
EagI CGGCCG 1 cut(s) 243
Eam1104I CTCTTC 1 cut(s) 104
EarI CTCTTC 1 cut(s) 104
EciI GGCGGA 2 cut(s) 16, 228
Ecl136II GAGCTC 1 cut(s) 135
EclXI CGGCCG 1 cut(s) 243
Eco130I CCWWGG 3 cut(s) 104, 342, 702
Eco24I GRGCYC 2 cut(s) 137, 191
Eco47I GGWCC 2 cut(s) 650, 670
Eco52I CGGCCG 1 cut(s) 243
Eco53kI GAGCTC 1 cut(s) 135
Eco57I CTGAAG 1 cut(s) 327
Eco81I CCTNAGG 1 cut(s) 718
Eco88I CYCGRG 1 cut(s) 258
EcoICRI GAGCTC 1 cut(s) 135
EcoRII CCWGG 1 cut(s) 190
EcoT14I CCWWGG 3 cut(s) 104, 342, 702
EcoT38I GRGCYC 2 cut(s) 137, 191
ErhI CCWWGG 3 cut(s) 104, 342, 702
FaeI CATG 6 cut(s) 80, 108, 395, 706, 742, 840
FaqI GGGAC 2 cut(s) 614, 656
FatI CATG 6 cut(s) 76, 104, 391, 702, 738, 836
FbaI TGATCA 1 cut(s) 388
FblI GTMKAC 1 cut(s) 745
Fnu4HI GCNGC 2 cut(s) 402, 644
FokI GGATG 3 cut(s) 51, 130, 182
FriOI GRGCYC 2 cut(s) 137, 191
Fsp4HI GCNGC 2 cut(s) 402, 644
FspAI RTGCGCAY 1 cut(s) 43
FspBI CTAG 4 cut(s) 165, 491, 795, 862
FspI TGCGCA 1 cut(s) 43
GlaI GCGC 1 cut(s) 43
GluI GCNGC 2 cut(s) 402, 644
GsaI CCCAGC 1 cut(s) 555
HaeIII GGCC 5 cut(s) 81, 189, 245, 341, 519
HapII CCGG 4 cut(s) 211, 242, 246, 259
HhaI GCGC 1 cut(s) 44
Hin1II CATG 6 cut(s) 80, 108, 395, 706, 742, 840
Hin6I GCGC 1 cut(s) 42
HinP1I GCGC 1 cut(s) 42
HpaII CCGG 4 cut(s) 211, 242, 246, 259
HphI GGTGA 2 cut(s) 398, 471
Hpy166II GTNNAC 3 cut(s) 533, 746, 854
Hpy188I TCNGA 5 cut(s) 98, 200, 296, 568, 666
Hpy188III TCNNGA 3 cut(s) 305, 456, 730
Hpy8I GTNNAC 3 cut(s) 533, 746, 854
HpyAV CCTTC 2 cut(s) 298, 708
HpyCH4III ACNGT 3 cut(s) 445, 654, 756
HpyCH4IV ACGT 1 cut(s) 73
HpyCH4V TGCA 3 cut(s) 128, 533, 808
HpyF10VI GCNNNNNNNGC 7 cut(s) 87, 125, 222, 491, 596, 623, 713
HpyF3I CTNAG 5 cut(s) 330, 448, 495, 718, 857
HpySE526I ACGT 1 cut(s) 73
Hsp92II CATG 6 cut(s) 80, 108, 395, 706, 742, 840
HspAI GCGC 1 cut(s) 42
KroI GCCGGC 2 cut(s) 241, 245
KroNI GCCGGC 2 cut(s) 243, 247
Ksp22I TGATCA 1 cut(s) 388
Kzo9I GATC 5 cut(s) 231, 388, 472, 732, 832
LguI GCTCTTC 1 cut(s) 104
LmnI GCTCC 3 cut(s) 213, 296, 555
Lsp1109I GCAGC 2 cut(s) 388, 655
LweI GCATC 3 cut(s) 29, 204, 695
MaeI CTAG 4 cut(s) 165, 491, 795, 862
MaeII ACGT 1 cut(s) 73
MaeIII GTNAC 3 cut(s) 7, 310, 379
MalI GATC 5 cut(s) 233, 390, 474, 734, 834
MbiI CCGCTC 1 cut(s) 216
MboI GATC 5 cut(s) 231, 388, 472, 732, 832
MboII GAAGA 3 cut(s) 12, 85, 121
MflI RGATCY 1 cut(s) 832
MhlI GDGCHC 4 cut(s) 137, 191, 535, 549
MlsI TGGCCA 3 cut(s) 81, 341, 519
MluCI AATT 2 cut(s) 32, 59
MluNI TGGCCA 3 cut(s) 81, 341, 519
MnlI CCTC 8 cut(s) 34, 66, 246, 340, 414, 463, 616, 727
Mox20I TGGCCA 3 cut(s) 81, 341, 519
MroNI GCCGGC 2 cut(s) 241, 245
MscI TGGCCA 3 cut(s) 81, 341, 519
MseI TTAA 1 cut(s) 660
MslI CAYNNNNRTG 1 cut(s) 525
Msp20I TGGCCA 3 cut(s) 81, 341, 519
MspI CCGG 4 cut(s) 211, 242, 246, 259
MspR9I CCNGG 4 cut(s) 192, 211, 259, 260
MvaI CCWGG 1 cut(s) 192
MwoI GCNNNNNNNGC 7 cut(s) 87, 125, 222, 491, 596, 623, 713
NaeI GCCGGC 2 cut(s) 243, 247
NciI CCSGG 3 cut(s) 211, 259, 260
NcoI CCATGG 2 cut(s) 104, 702
NdeII GATC 5 cut(s) 231, 388, 472, 732, 832
NgoMIV GCCGGC 2 cut(s) 241, 245
NlaIII CATG 6 cut(s) 80, 108, 395, 706, 742, 840
NlaIV GGNNCC 3 cut(s) 189, 286, 672
NmuCI GTSAC 2 cut(s) 310, 379
NsbI TGCGCA 1 cut(s) 43
NspI RCATGY 1 cut(s) 742
PaqCI CACCTGC 1 cut(s) 490
PciI ACATGT 1 cut(s) 738
PciSI GCTCTTC 1 cut(s) 104
PdiI GCCGGC 2 cut(s) 243, 247
PflFI GACNNNGTC 1 cut(s) 443
PkrI GCNGC 2 cut(s) 403, 645
PscI ACATGT 1 cut(s) 738
Psp124BI GAGCTC 1 cut(s) 137
Psp6I CCWGG 1 cut(s) 190
PspFI CCCAGC 1 cut(s) 551
PspGI CCWGG 1 cut(s) 190
PspN4I GGNNCC 3 cut(s) 189, 286, 672
PspOMI GGGCCC 1 cut(s) 187
PspPI GGNCC 4 cut(s) 187, 188, 650, 670
PstNI CAGNNNCTG 1 cut(s) 626
PsuI RGATCY 1 cut(s) 832
PsyI GACNNNGTC 1 cut(s) 443
RseI CAYNNNNRTG 1 cut(s) 525
SacI GAGCTC 1 cut(s) 137
SapI GCTCTTC 1 cut(s) 104
SaqAI TTAA 1 cut(s) 660
SatI GCNGC 2 cut(s) 402, 644
Sau3AI GATC 5 cut(s) 231, 388, 472, 732, 832
Sau96I GGNCC 4 cut(s) 187, 188, 650, 670
ScrFI CCNGG 4 cut(s) 192, 211, 259, 260
SduI GDGCHC 4 cut(s) 137, 191, 535, 549
SfaNI GCATC 3 cut(s) 29, 204, 695
SinI GGWCC 2 cut(s) 650, 670
SmaI CCCGGG 1 cut(s) 260
SmiMI CAYNNNNRTG 1 cut(s) 525
SpeI ACTAGT 1 cut(s) 164
Sse9I AATT 2 cut(s) 32, 59
SsiI CCGC 4 cut(s) 27, 216, 239, 282
SspMI CTAG 4 cut(s) 165, 491, 795, 862
SstI GAGCTC 1 cut(s) 137
StyD4I CCNGG 4 cut(s) 190, 209, 257, 258
StyI CCWWGG 3 cut(s) 104, 342, 702
TaaI ACNGT 3 cut(s) 445, 654, 756
TaiI ACGT 1 cut(s) 76
TaqI TCGA 1 cut(s) 735
TasI AATT 2 cut(s) 32, 59
Tru1I TTAA 1 cut(s) 660
Tru9I TTAA 1 cut(s) 660
TscAI CASTG 3 cut(s) 135, 372, 510
TseFI GTSAC 2 cut(s) 310, 379
TseI GCWGC 2 cut(s) 401, 643
Tsp45I GTSAC 2 cut(s) 310, 379
TspDTI ATGAA 2 cut(s) 132, 280
TspMI CCCGGG 1 cut(s) 258
TspRI CASTG 3 cut(s) 135, 372, 510
Tth111I GACNNNGTC 1 cut(s) 443
VneI GTGCAC 1 cut(s) 531
VpaK11BI GGWCC 2 cut(s) 650, 670
XapI RAATTY 1 cut(s) 32
XceI RCATGY 1 cut(s) 742
XcmI CCANNNNNNNNNTGG 1 cut(s) 548
XmaI CCCGGG 1 cut(s) 258
XmiI GTMKAC 1 cut(s) 745
XspI CTAG 4 cut(s) 165, 491, 795, 862
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.