pycom08g03940

Protein of unknown function (DUF1350)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0013928
Physical Location & Seq
Reverse (-)
2977542 .. 2980732
3191 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g03940.6

Sequence Viewer

Length: 1242 bp
ATGTGGGCTTTGGACATGGAAATCGCCGCCTGTTTCTCCTCCTCCGCCGGCTTTGCCGCCAAATTTGGATGCCTGTACGATCACATCCCACTTCACGCCCATACCCATATCAACGGCTATGGTTCCTTCTTGGGATTTCGCGGGAGTAGCAGAGGCAGCAGAGCAGTGGTCGCAGGCGGCTCAGCCTCGGCCTCCGCCAAGAAGCTGGCCGTCAACGGATACGCTGGTCCCAGTTCCACCACTAGCAGGTTAACCTACACTTGGACCGATTCCTGTCTGGTGATTCCTCCGCCCGCCGGCAAGAAGCCACGCGCCATCATCAAGTTCTTGGGTGGCGCCTTCATCGGAGCTGTTCCAGAACTTACTTACAGCTACTTAACTGAGCTATTGGCAAAGGATGGGTTTCTGGTGATATCAGTGCCGTACAATGTGACGTTTGATCATGTCCAAGCTTCCCGGCAAGTGTATGACAGGTTCAATGGCTGCTTGGACAACATCTTAGCTTCTGGACTGCCCAATTCCAATCTATCAGCTGCCCAACTTGCTCAACTGCCCGTGTTTTCGGTTGGCCATAGCAATGGTGCCCTTCTTCAAGTACTTGCTGGGAGCTATTTCTCTGATAAAATCCCTAAGGCCAATGCGATAATAGCATACAATAATAGACCAGCAACAGAGGCCGTGCCTTACTTTGAGCAGTTAGGTCCTTTAGTTAATCAAATGGTGCCTATTATAGAAGCATCTCCAGTTTCTTCCGTGGCAAGGACTGCCTCAGGAGATGCATGGAAGGCGCTGATTGGTGCAGCTGGGGCTATGCTACCAGACAATCAGGAAACTCTAAGTTCGGTGACTAAATTTGTTGATCAGTTGCCCTCAGTGCTAAATGAGGTAGCACAAGGGATATCAGAGTTTAAGCCGACACCCTCTGAAAACCGTGATTTTTGTAAGAGCTCGTACAATGTCAAACACACGCTACTGGTGAAGTTCAATTTTGACGCAATTGATGAGACAGATACTCTTGAAGAGACGTTAAAGCCTCGCGTGGAATCTATTGGCGGAACACTAGAAAAGGTCGAAATAAGTGGTAACCATATTACTCCATGCGTACAGGAACCTAAGTGGCAAGCAGGTTACGTTTACACTCCAGCAGATGCTATTGCTCAGAGTCTTAAGACGCTTTCGCTAAATGACGTTAGAGTCCTTTCGAGAACTATAAGCGACTGGTTCAGAGGCTTTGAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1350

Protein Analysis

414

Amino Acids

44.34

Weight (kDa)

6.09

Isoelectric Point (pI)

33.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013928)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G43540 AT3G43540
fragaria_vesca FvH4_2g37050 FvH4_2g37050
malus_domestica MD08G1047000.v1.1
pyrus_communis pycom08g03940
rosa_chinensis RchiOBHm_Chr6g0304731
rosa_laevigata RLG00000010955
rosa_multiflora Rmu_co8515209.1_g000001
rosa_roxburghii Rroxscaffold_7G00163550
rosa_rugosa Rorug06G0333900 Rorug06G0334000
rosa_samantha Rh6AG445900 Rh6BG455800 Rh6CG459000 Rh6DG446000
rosa_wichuraiana Rw6G038790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1193
Acc36I ACCTGC 2 cut(s) 237, 1115
AccB1I GGYRCC 3 cut(s) 335, 581, 721
AccII CGCG 3 cut(s) 141, 312, 1038
AciI CCGC 9 cut(s) 27, 45, 57, 141, 177, 195, 290, 294, 1053
AcoI YGGCCR 2 cut(s) 207, 568
AcsI RAATTY 2 cut(s) 62, 851
AcyI GRCGYC 1 cut(s) 336
AfaI GTAC 5 cut(s) 77, 425, 597, 953, 1104
AfiI CCNNNNNNNGG 4 cut(s) 246, 261, 296, 759
AflII CTTAAG 1 cut(s) 1166
AgsI TTSAA 5 cut(s) 478, 593, 985, 1019, 1235
Alw21I GWGCWC 1 cut(s) 950
Alw26I GTCTC 2 cut(s) 998, 1016
AoxI GGCC 5 cut(s) 189, 207, 568, 633, 675
ApeKI GCWGC 4 cut(s) 156, 483, 533, 800
ApoI RAATTY 2 cut(s) 62, 851
AspLEI GCGC 3 cut(s) 314, 338, 790
AspS9I GGNCC 3 cut(s) 227, 264, 701
AsuC2I CCSGG 1 cut(s) 457
AsuHPI GGTGA 4 cut(s) 292, 421, 856, 988
AvaII GGWCC 3 cut(s) 227, 264, 701
AxyI CCTNAGG 2 cut(s) 630, 769
BaeGI GKGCMC 1 cut(s) 586
BaeI ACNNNNGTAYC 2 cut(s) 211, 244
BalI TGGCCA 1 cut(s) 570
BanI GGYRCC 3 cut(s) 335, 581, 721
BanII GRGCYC 1 cut(s) 950
Bbv12I GWGCWC 1 cut(s) 950
BbvI GCAGC 4 cut(s) 168, 470, 520, 812
BccI CCATC 2 cut(s) 323, 392
BceAI ACGGC 4 cut(s) 130, 194, 406, 662
BciVI GTATCC 1 cut(s) 212
BclI TGATCA 2 cut(s) 439, 859
BcnI CCSGG 1 cut(s) 457
BcoDI GTCTC 2 cut(s) 998, 1016
BfaI CTAG 2 cut(s) 243, 1061
BfoI RGCGCY 2 cut(s) 339, 791
BfrI CTTAAG 1 cut(s) 1166
BfuAI ACCTGC 2 cut(s) 237, 1115
BfuI GTATCC 1 cut(s) 212
BisI GCNGC 7 cut(s) 27, 57, 157, 178, 484, 534, 801
BlpI GCTNAGC 1 cut(s) 181
BlsI GCNGC 7 cut(s) 28, 58, 158, 179, 485, 535, 802
BmcAI AGTACT 1 cut(s) 597
Bme1390I CCNGG 1 cut(s) 457
Bme18I GGWCC 3 cut(s) 227, 264, 701
BmgT120I GGNCC 3 cut(s) 227, 264, 701
BmiI GGNNCC 6 cut(s) 124, 229, 337, 583, 723, 1110
BmrFI CCNGG 1 cut(s) 457
BmrI ACTGGG 1 cut(s) 225
BmsI GCATC 4 cut(s) 59, 746, 766, 1138
BmuI ACTGGG 1 cut(s) 225
BpmI CTGGAG 2 cut(s) 726, 1125
Bpu1102I GCTNAGC 1 cut(s) 181
BpuMI CCSGG 1 cut(s) 457
BsaHI GRCGYC 1 cut(s) 336
BsaJI CCNNGG 2 cut(s) 186, 753
Bsc4I CCNNNNNNNGG 4 cut(s) 246, 261, 296, 759
Bse118I RCCGGY 2 cut(s) 47, 296
Bse1I ACTGG 4 cut(s) 231, 743, 978, 1223
Bse21I CCTNAGG 2 cut(s) 630, 769
Bse3DI GCAATG 1 cut(s) 583
BseDI CCNNGG 2 cut(s) 186, 753
BseGI GGATG 3 cut(s) 74, 84, 403
BseLI CCNNNNNNNGG 4 cut(s) 246, 261, 296, 759
BseMI GCAATG 1 cut(s) 583
BseMII CTCAG 5 cut(s) 195, 372, 783, 885, 1172
BseNI ACTGG 4 cut(s) 231, 743, 978, 1223
BseRI GAGGAG 2 cut(s) 28, 31
BseSI GKGCMC 1 cut(s) 586
BseXI GCAGC 4 cut(s) 168, 470, 520, 812
BseYI CCCAGC 2 cut(s) 602, 803
BsgI GTGCAG 1 cut(s) 819
Bsh1236I CGCG 3 cut(s) 141, 312, 1038
BshFI GGCC 5 cut(s) 191, 209, 570, 635, 677
BshNI GGYRCC 3 cut(s) 335, 581, 721
BsiHKAI GWGCWC 1 cut(s) 950
BsiSI CCGG 3 cut(s) 48, 297, 457
BslFI GGGAC 1 cut(s) 213
BslI CCNNNNNNNGG 4 cut(s) 246, 261, 296, 759
BsmAI GTCTC 2 cut(s) 998, 1016
BsmBI CGTCTC 1 cut(s) 1016
BsmFI GGGAC 1 cut(s) 213
BsnI GGCC 5 cut(s) 191, 209, 570, 635, 677
Bsp1286I GDGCHC 2 cut(s) 586, 950
Bsp143I GATC 3 cut(s) 79, 439, 859
Bsp1720I GCTNAGC 1 cut(s) 181
BspACI CCGC 9 cut(s) 27, 45, 57, 141, 177, 195, 290, 294, 1053
BspANI GGCC 5 cut(s) 191, 209, 570, 635, 677
BspCNI CTCAG 5 cut(s) 194, 373, 782, 884, 1171
BspFNI CGCG 3 cut(s) 141, 312, 1038
BspLI GGNNCC 6 cut(s) 124, 229, 337, 583, 723, 1110
BspMI ACCTGC 2 cut(s) 237, 1115
BspT107I GGYRCC 3 cut(s) 335, 581, 721
BspTI CTTAAG 1 cut(s) 1166
BsrDI GCAATG 1 cut(s) 583
BsrFI RCCGGY 2 cut(s) 47, 296
BsrI ACTGG 4 cut(s) 231, 743, 978, 1223
BssAI RCCGGY 2 cut(s) 47, 296
BssECI CCNNGG 2 cut(s) 186, 753
BssMI GATC 3 cut(s) 79, 439, 859
BssNI GRCGYC 1 cut(s) 336
Bst4CI ACNGT 1 cut(s) 932
Bst6I CTCTTC 1 cut(s) 1014
BstACI GRCGYC 1 cut(s) 336
BstAFI CTTAAG 1 cut(s) 1166
BstAPI GCANNNNNTGC 1 cut(s) 764
BstC8I GCNNGC 6 cut(s) 49, 175, 207, 294, 298, 1122
BstDEI CTNAG 9 cut(s) 181, 381, 499, 630, 769, 836, 871, 1113, 1158
BstDSI CCRYGG 1 cut(s) 753
BstEII GGTNACC 1 cut(s) 1082
BstF5I GGATG 3 cut(s) 74, 84, 403
BstFNI CGCG 3 cut(s) 141, 312, 1038
BstH2I RGCGCY 2 cut(s) 339, 791
BstHHI GCGC 3 cut(s) 314, 338, 790
BstKTI GATC 3 cut(s) 82, 442, 862
BstMAI GTCTC 2 cut(s) 998, 1016
BstMBI GATC 3 cut(s) 79, 439, 859
BstPI GGTNACC 1 cut(s) 1082
BstSCI CCNGG 1 cut(s) 455
BstSLI GKGCMC 1 cut(s) 586
BstUI CGCG 3 cut(s) 141, 312, 1038
BstV1I GCAGC 4 cut(s) 168, 470, 520, 812
BstXI CCANNNNNNTGG 2 cut(s) 205, 578
Bsu36I CCTNAGG 2 cut(s) 630, 769
BsuI GTATCC 1 cut(s) 212
BsuRI GGCC 5 cut(s) 191, 209, 570, 635, 677
BtgI CCRYGG 1 cut(s) 753
BtsCI GGATG 3 cut(s) 74, 84, 403
BtsI GCAGTG 1 cut(s) 171
BtsIMutI CAGTG 3 cut(s) 171, 423, 879
BveI ACCTGC 2 cut(s) 237, 1115
Cac8I GCNNGC 6 cut(s) 49, 175, 207, 294, 298, 1122
CfoI GCGC 3 cut(s) 314, 338, 790
Cfr10I RCCGGY 2 cut(s) 47, 296
Cfr13I GGNCC 3 cut(s) 227, 264, 701
CseI GACGC 2 cut(s) 1001, 1180
Csp6I GTAC 5 cut(s) 76, 424, 596, 952, 1103
CviAII CATG 4 cut(s) 16, 443, 780, 1098
CviQI GTAC 5 cut(s) 76, 424, 596, 952, 1103
DdeI CTNAG 9 cut(s) 181, 381, 499, 630, 769, 836, 871, 1113, 1158
DinI GGCGCC 1 cut(s) 337
DpnI GATC 3 cut(s) 81, 441, 861
DpnII GATC 3 cut(s) 79, 439, 859
DrdI GACNNNNNNGTC 1 cut(s) 1193
DseDI GACNNNNNNGTC 1 cut(s) 1193
EaeI YGGCCR 2 cut(s) 207, 568
Eam1104I CTCTTC 1 cut(s) 1014
EarI CTCTTC 1 cut(s) 1014
EciI GGCGGA 4 cut(s) 34, 184, 279, 1068
Ecl136II GAGCTC 1 cut(s) 948
Eco24I GRGCYC 1 cut(s) 950
Eco32I GATATC 2 cut(s) 414, 900
Eco47I GGWCC 3 cut(s) 227, 264, 701
Eco53kI GAGCTC 1 cut(s) 948
Eco81I CCTNAGG 2 cut(s) 630, 769
Eco91I GGTNACC 1 cut(s) 1082
EcoICRI GAGCTC 1 cut(s) 948
EcoO109I RGGNCCY 1 cut(s) 701
EcoO65I GGTNACC 1 cut(s) 1082
EcoRV GATATC 2 cut(s) 414, 900
EcoT22I ATGCAT 1 cut(s) 781
EcoT38I GRGCYC 1 cut(s) 950
EgeI GGCGCC 1 cut(s) 337
EheI GGCGCC 1 cut(s) 337
Esp3I CGTCTC 1 cut(s) 1016
FaeI CATG 4 cut(s) 19, 446, 783, 1101
FaqI GGGAC 1 cut(s) 213
FatI CATG 4 cut(s) 15, 442, 779, 1097
FauI CCCGC 2 cut(s) 134, 301
FbaI TGATCA 2 cut(s) 439, 859
Fnu4HI GCNGC 7 cut(s) 27, 57, 157, 178, 484, 534, 801
FokI GGATG 3 cut(s) 71, 81, 410
FriOI GRGCYC 1 cut(s) 950
Fsp4HI GCNGC 7 cut(s) 27, 57, 157, 178, 484, 534, 801
FspBI CTAG 2 cut(s) 243, 1061
GlaI GCGC 3 cut(s) 313, 337, 789
GluI GCNGC 7 cut(s) 27, 57, 157, 178, 484, 534, 801
GsaI CCCAGC 2 cut(s) 606, 807
GsuI CTGGAG 2 cut(s) 726, 1125
HaeII RGCGCY 2 cut(s) 339, 791
HaeIII GGCC 5 cut(s) 191, 209, 570, 635, 677
HapII CCGG 3 cut(s) 48, 297, 457
HgaI GACGC 2 cut(s) 1001, 1180
HhaI GCGC 3 cut(s) 314, 338, 790
Hin1I GRCGYC 1 cut(s) 336
Hin1II CATG 4 cut(s) 19, 446, 783, 1101
Hin6I GCGC 3 cut(s) 312, 336, 788
HinP1I GCGC 3 cut(s) 312, 336, 788
HincII GTYRAC 2 cut(s) 214, 252
HindII GTYRAC 2 cut(s) 214, 252
HindIII AAGCTT 1 cut(s) 450
HinfI GANTC 5 cut(s) 269, 283, 1043, 1162, 1194
HpaI GTTAAC 1 cut(s) 252
HpaII CCGG 3 cut(s) 48, 297, 457
HphI GGTGA 4 cut(s) 292, 421, 856, 988
Hpy166II GTNNAC 3 cut(s) 214, 252, 1135
Hpy188I TCNGA 6 cut(s) 347, 619, 904, 925, 1161, 1226
Hpy188III TCNNGA 6 cut(s) 356, 507, 771, 827, 1016, 1203
Hpy8I GTNNAC 3 cut(s) 214, 252, 1135
HpyAV CCTTC 4 cut(s) 136, 349, 596, 778
HpyCH4III ACNGT 1 cut(s) 932
HpyCH4IV ACGT 4 cut(s) 434, 1025, 1131, 1188
HpyCH4V TGCA 2 cut(s) 779, 800
HpyF3I CTNAG 9 cut(s) 181, 381, 499, 630, 769, 836, 871, 1113, 1158
HpySE526I ACGT 4 cut(s) 434, 1025, 1131, 1188
Hsp92I GRCGYC 1 cut(s) 336
Hsp92II CATG 4 cut(s) 19, 446, 783, 1101
HspAI GCGC 3 cut(s) 312, 336, 788
KasI GGCGCC 1 cut(s) 335
KroI GCCGGC 2 cut(s) 47, 296
KroNI GCCGGC 2 cut(s) 49, 298
Ksp22I TGATCA 2 cut(s) 439, 859
KspAI GTTAAC 1 cut(s) 252
Kzo9I GATC 3 cut(s) 79, 439, 859
LmnI GCTCC 2 cut(s) 347, 606
Lsp1109I GCAGC 4 cut(s) 168, 470, 520, 812
LweI GCATC 4 cut(s) 59, 746, 766, 1138
MaeI CTAG 2 cut(s) 243, 1061
MaeII ACGT 4 cut(s) 434, 1025, 1131, 1188
MaeIII GTNAC 4 cut(s) 430, 844, 1082, 1127
MalI GATC 3 cut(s) 81, 441, 861
MboI GATC 3 cut(s) 79, 439, 859
MboII GAAGA 3 cut(s) 581, 741, 1031
MfeI CAATTG 1 cut(s) 996
MhlI GDGCHC 2 cut(s) 586, 950
MlsI TGGCCA 1 cut(s) 570
MluCI AATT 5 cut(s) 62, 517, 851, 985, 996
MluNI TGGCCA 1 cut(s) 570
Mly113I GGCGCC 1 cut(s) 336
MlyI GAGTC 2 cut(s) 1171, 1203
Mox20I TGGCCA 1 cut(s) 570
Mph1103I ATGCAT 1 cut(s) 781
MroNI GCCGGC 2 cut(s) 47, 296
MscI TGGCCA 1 cut(s) 570
MseI TTAA 6 cut(s) 251, 377, 711, 909, 1028, 1167
MslI CAYNNNNRTG 1 cut(s) 576
Msp20I TGGCCA 1 cut(s) 570
MspA1I CMGCKG 2 cut(s) 533, 803
MspCI CTTAAG 1 cut(s) 1166
MspI CCGG 3 cut(s) 48, 297, 457
MspR9I CCNGG 1 cut(s) 457
MunI CAATTG 1 cut(s) 996
MvnI CGCG 3 cut(s) 141, 312, 1038
NaeI GCCGGC 2 cut(s) 49, 298
NarI GGCGCC 1 cut(s) 336
NciI CCSGG 1 cut(s) 457
NdeII GATC 3 cut(s) 79, 439, 859
NgoMIV GCCGGC 2 cut(s) 47, 296
NlaIII CATG 4 cut(s) 19, 446, 783, 1101
NlaIV GGNNCC 6 cut(s) 124, 229, 337, 583, 723, 1110
NmeAIII GCCGAG 1 cut(s) 167
NmuCI GTSAC 2 cut(s) 430, 844
NsiI ATGCAT 1 cut(s) 781
PdiI GCCGGC 2 cut(s) 49, 298
PfeI GAWTC 3 cut(s) 269, 283, 1043
PkrI GCNGC 7 cut(s) 28, 58, 158, 179, 485, 535, 802
PleI GAGTC 2 cut(s) 1170, 1202
PluTI GGCGCC 1 cut(s) 339
PpsI GAGTC 2 cut(s) 1170, 1202
PpuMI RGGWCCY 1 cut(s) 701
Psp124BI GAGCTC 1 cut(s) 950
Psp5II RGGWCCY 1 cut(s) 701
PspEI GGTNACC 1 cut(s) 1082
PspFI CCCAGC 2 cut(s) 602, 803
PspN4I GGNNCC 6 cut(s) 124, 229, 337, 583, 723, 1110
PspPI GGNCC 3 cut(s) 227, 264, 701
PspPPI RGGWCCY 1 cut(s) 701
PsrI GAACNNNNNNTAC 2 cut(s) 458, 490
PvuII CAGCTG 2 cut(s) 533, 803
RsaI GTAC 5 cut(s) 77, 425, 597, 953, 1104
RsaNI GTAC 5 cut(s) 76, 424, 596, 952, 1103
RseI CAYNNNNRTG 1 cut(s) 576
SacI GAGCTC 1 cut(s) 950
SaqAI TTAA 6 cut(s) 251, 377, 711, 909, 1028, 1167
SatI GCNGC 7 cut(s) 27, 57, 157, 178, 484, 534, 801
Sau3AI GATC 3 cut(s) 79, 439, 859
Sau96I GGNCC 3 cut(s) 227, 264, 701
ScaI AGTACT 1 cut(s) 597
SchI GAGTC 2 cut(s) 1171, 1203
ScrFI CCNGG 1 cut(s) 457
SduI GDGCHC 2 cut(s) 586, 950
SfaNI GCATC 4 cut(s) 59, 746, 766, 1138
SfoI GGCGCC 1 cut(s) 337
SinI GGWCC 3 cut(s) 227, 264, 701
SmiMI CAYNNNNRTG 1 cut(s) 576
SmlI CTYRAG 1 cut(s) 1166
SmoI CTYRAG 1 cut(s) 1166
Sse9I AATT 5 cut(s) 62, 517, 851, 985, 996
SsiI CCGC 9 cut(s) 27, 45, 57, 141, 177, 195, 290, 294, 1053
SspDI GGCGCC 1 cut(s) 335
SspMI CTAG 2 cut(s) 243, 1061
SstI GAGCTC 1 cut(s) 950
StyD4I CCNGG 1 cut(s) 455
TaaI ACNGT 1 cut(s) 932
TaiI ACGT 4 cut(s) 437, 1028, 1134, 1191
TaqI TCGA 2 cut(s) 1071, 1202
TaqII GACCGA 1 cut(s) 281
TasI AATT 5 cut(s) 62, 517, 851, 985, 996
TatI WGTACW 1 cut(s) 595
TauI GCSGC 3 cut(s) 29, 59, 180
TfiI GAWTC 3 cut(s) 269, 283, 1043
Tru1I TTAA 6 cut(s) 251, 377, 711, 909, 1028, 1167
Tru9I TTAA 6 cut(s) 251, 377, 711, 909, 1028, 1167
TscAI CASTG 3 cut(s) 171, 423, 879
TseFI GTSAC 2 cut(s) 430, 844
TseI GCWGC 4 cut(s) 156, 483, 533, 800
Tsp45I GTSAC 2 cut(s) 430, 844
TspDTI ATGAA 1 cut(s) 331
TspGWI ACGGA 2 cut(s) 231, 742
TspRI CASTG 3 cut(s) 171, 423, 879
Vha464I CTTAAG 1 cut(s) 1166
VpaK11BI GGWCC 3 cut(s) 227, 264, 701
XapI RAATTY 2 cut(s) 62, 851
XspI CTAG 2 cut(s) 243, 1061
ZrmI AGTACT 1 cut(s) 597
Zsp2I ATGCAT 1 cut(s) 781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.