AT3G44960

Shugoshin C terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009270
Physical Location & Seq
Forward (+)
16427581 .. 16429403
1823 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G44960.1

Sequence Viewer

Length: 810 bp
ATGATGAAAAGATCTCCATTTCGTCACAAAATGAGGCAGAGTCTCTCTGATATTACTAACTCTCAGTCTCAAGAAGAGCTTAATCTTCACAAGATCGGTTCCGAGCCGAGTGGACATGTAAACCGGCTGATGAAGGAGAATTTAGCATTGGTTAAGCTTCTTGAGGAAAGAGATAAAACAATTGATATAAGTAGATACGAATTGCGGAATTTACGCGAAGGCATACACAAACTTCAAGTACAAAATTGGAGCCTTGCTCAATCCAATTCCCAATTCTTAGGCGAGATCAATTTAGCTAGAAACAAGGTGAAAGCGTTGCACCATGAGGTTACTTGCAAAAATGCTTTGCTAAAGGCTAAATGTTGTGACAAAGAGAAAGGTGAAAACACTAAACCAAGAAATGCACTAACAACTGAGAGTGTGCTAAATATAACGGACGAAGACTCACCGTCACCGAAACCTTTTGTACCAACCAGAAGGCGATTCATCAGAAGCAAATCCTTAGGAGCATCTACTACAAACAAGAATGAGGCAGAAAAAGTGAAATCGGAAACCAAAAGGAGATCGCCGAGAGTGAGATCAGCAGCGCAAGAAGTGACAGAGAATCTGTTTGAGATTGAAGATCTCCAGCTTACAATGCCTAATGACATGTCTCAACAAGATAACTTGACAATGGAATCACATACCCGAAAGAAAGAAGAGGGGAAAGAAGGTTTACGGACAAGACATCATGATTTTGAGATCTATCGATCACCAGTCGATCGATCATTGCGTAGAGAAGCTGAGAGGATACACTCTTACAAGGGATAA

Protein Analysis

269

Amino Acids

31.19

Weight (kDa)

9.64

Isoelectric Point (pI)

77.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAP1_N PF04849 16 - 132 5.7e-06 HAP1 N-terminal conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 216
AciI CCGC 1 cut(s) 205
AcsI RAATTY 2 cut(s) 139, 208
AfaI GTAC 2 cut(s) 240, 468
AflIII ACRYGT 2 cut(s) 115, 648
AgsI TTSAA 2 cut(s) 236, 620
AhdI GACNNNNNGTC 1 cut(s) 448
AjuI GAANNNNNNNTTGG 4 cut(s) 131, 163, 257, 289
AluBI AGCT 5 cut(s) 79, 157, 296, 631, 782
AluI AGCT 5 cut(s) 79, 157, 296, 631, 782
Alw26I GTCTC 3 cut(s) 47, 72, 657
ApeKI GCWGC 1 cut(s) 584
ApoI RAATTY 2 cut(s) 139, 208
AspLEI GCGC 1 cut(s) 589
AsuHPI GGTGA 5 cut(s) 319, 392, 438, 444, 744
AxyI CCTNAGG 1 cut(s) 502
BbsI GAAGAC 1 cut(s) 447
BbvI GCAGC 1 cut(s) 596
BciVI GTATCC 1 cut(s) 783
BcoDI GTCTC 3 cut(s) 47, 72, 657
BfaI CTAG 1 cut(s) 297
BfuI GTATCC 1 cut(s) 783
BglII AGATCT 3 cut(s) 11, 622, 741
BisI GCNGC 1 cut(s) 585
BlsI GCNGC 1 cut(s) 586
BmeRI GACNNNNNGTC 1 cut(s) 448
BmiI GGNNCC 2 cut(s) 100, 251
BmsI GCATC 1 cut(s) 518
BpiI GAAGAC 1 cut(s) 447
BplI GAGNNNNNCTC 2 cut(s) 241, 273
BpmI CTGGAG 1 cut(s) 611
BpuEI CTTGAG 2 cut(s) 54, 182
Bsa29I ATCGAT 2 cut(s) 748, 763
Bse118I RCCGGY 1 cut(s) 123
Bse1I ACTGG 1 cut(s) 755
Bse21I CCTNAGG 1 cut(s) 502
Bse3DI GCAATG 1 cut(s) 767
BseCI ATCGAT 2 cut(s) 748, 763
BseMI GCAATG 1 cut(s) 767
BseMII CTCAG 3 cut(s) 77, 405, 774
BseNI ACTGG 1 cut(s) 755
BseXI GCAGC 1 cut(s) 596
Bsh1236I CGCG 1 cut(s) 216
Bsh1285I CGRYCG 1 cut(s) 763
BshVI ATCGAT 2 cut(s) 748, 763
BsiEI CGRYCG 1 cut(s) 763
BsiSI CCGG 1 cut(s) 124
BsmAI GTCTC 3 cut(s) 47, 72, 657
BspACI CCGC 1 cut(s) 205
BspCNI CTCAG 3 cut(s) 76, 406, 775
BspDI ATCGAT 2 cut(s) 748, 763
BspFNI CGCG 1 cut(s) 216
BspHI TCATGA 1 cut(s) 730
BspLI GGNNCC 2 cut(s) 100, 251
BspQI GCTCTTC 1 cut(s) 69
BsrDI GCAATG 1 cut(s) 767
BsrFI RCCGGY 1 cut(s) 123
BsrI ACTGG 1 cut(s) 755
BssAI RCCGGY 1 cut(s) 123
Bst4CI ACNGT 1 cut(s) 450
Bst6I CTCTTC 2 cut(s) 69, 693
BstDEI CTNAG 5 cut(s) 63, 277, 414, 502, 783
BstFNI CGCG 1 cut(s) 216
BstHHI GCGC 1 cut(s) 589
BstMAI GTCTC 3 cut(s) 47, 72, 657
BstMCI CGRYCG 1 cut(s) 763
BstMWI GCNNNNNNNGC 1 cut(s) 637
BstNSI RCATGY 2 cut(s) 119, 652
BstUI CGCG 1 cut(s) 216
BstV1I GCAGC 1 cut(s) 596
BstV2I GAAGAC 1 cut(s) 447
BstX2I RGATCY 3 cut(s) 11, 622, 741
BstYI RGATCY 3 cut(s) 11, 622, 741
Bsu15I ATCGAT 2 cut(s) 748, 763
Bsu36I CCTNAGG 1 cut(s) 502
BsuI GTATCC 1 cut(s) 783
BsuTUI ATCGAT 2 cut(s) 748, 763
CciI TCATGA 1 cut(s) 730
CfoI GCGC 1 cut(s) 589
Cfr10I RCCGGY 1 cut(s) 123
ClaI ATCGAT 2 cut(s) 748, 763
Csp6I GTAC 2 cut(s) 239, 467
CviAII CATG 4 cut(s) 116, 323, 649, 731
CviJI RGCY 9 cut(s) 79, 106, 127, 157, 252, 296, 356, 631, 782
CviKI_1 RGCY 9 cut(s) 79, 106, 127, 157, 252, 296, 356, 631, 782
CviQI GTAC 2 cut(s) 239, 467
DdeI CTNAG 5 cut(s) 63, 277, 414, 502, 783
DriI GACNNNNNGTC 1 cut(s) 448
Eam1104I CTCTTC 2 cut(s) 69, 693
Eam1105I GACNNNNNGTC 1 cut(s) 448
EarI CTCTTC 2 cut(s) 69, 693
Eco81I CCTNAGG 1 cut(s) 502
FaeI CATG 4 cut(s) 119, 326, 652, 734
FaiI YATR 8 cut(s) 117, 188, 224, 324, 431, 650, 684, 732
FalI AAGNNNNNCTT 2 cut(s) 63, 95
FatI CATG 4 cut(s) 115, 322, 648, 730
Fnu4HI GCNGC 1 cut(s) 585
Fsp4HI GCNGC 1 cut(s) 585
FspBI CTAG 1 cut(s) 297
GlaI GCGC 1 cut(s) 588
GluI GCNGC 1 cut(s) 585
GsuI CTGGAG 1 cut(s) 611
HapII CCGG 1 cut(s) 124
HhaI GCGC 1 cut(s) 589
Hin1II CATG 4 cut(s) 119, 326, 652, 734
Hin6I GCGC 1 cut(s) 587
HinP1I GCGC 1 cut(s) 587
HindIII AAGCTT 1 cut(s) 155
HinfI GANTC 5 cut(s) 40, 443, 483, 604, 677
HpaII CCGG 1 cut(s) 124
HphI GGTGA 5 cut(s) 319, 392, 438, 444, 744
Hpy166II GTNNAC 3 cut(s) 113, 121, 716
Hpy188I TCNGA 4 cut(s) 49, 103, 491, 550
Hpy188III TCNNGA 3 cut(s) 71, 161, 731
Hpy8I GTNNAC 3 cut(s) 113, 121, 716
HpyAV CCTTC 4 cut(s) 127, 212, 471, 704
HpyCH4III ACNGT 1 cut(s) 450
HpyCH4V TGCA 3 cut(s) 319, 336, 404
HpyF10VI GCNNNNNNNGC 1 cut(s) 637
HpyF3I CTNAG 5 cut(s) 63, 277, 414, 502, 783
Hsp92II CATG 4 cut(s) 119, 326, 652, 734
HspAI GCGC 1 cut(s) 587
LguI GCTCTTC 1 cut(s) 69
LmnI GCTCC 2 cut(s) 249, 506
LpnPI CCDG 4 cut(s) 137, 487, 641, 768
Lsp1109I GCAGC 1 cut(s) 596
LweI GCATC 1 cut(s) 518
MaeI CTAG 1 cut(s) 297
MaeIII GTNAC 5 cut(s) 23, 328, 365, 450, 595
MboII GAAGA 5 cut(s) 77, 86, 452, 632, 710
MfeI CAATTG 1 cut(s) 180
MflI RGATCY 3 cut(s) 11, 622, 741
MluCI AATT 8 cut(s) 139, 180, 200, 208, 244, 265, 272, 289
MlyI GAGTC 2 cut(s) 49, 437
MnlI CCTC 6 cut(s) 27, 157, 319, 523, 694, 780
MseI TTAA 2 cut(s) 81, 153
MspI CCGG 1 cut(s) 124
MunI CAATTG 1 cut(s) 180
MvnI CGCG 1 cut(s) 216
MwoI GCNNNNNNNGC 1 cut(s) 637
NlaIII CATG 4 cut(s) 119, 326, 652, 734
NlaIV GGNNCC 2 cut(s) 100, 251
NmeAIII GCCGAG 2 cut(s) 132, 594
NmuCI GTSAC 4 cut(s) 23, 365, 450, 595
NspI RCATGY 2 cut(s) 119, 652
PagI TCATGA 1 cut(s) 730
PciI ACATGT 2 cut(s) 115, 648
PciSI GCTCTTC 1 cut(s) 69
PcsI WCGNNNNNNNCGW 1 cut(s) 769
PfeI GAWTC 3 cut(s) 483, 604, 677
PkrI GCNGC 1 cut(s) 586
Ple19I CGATCG 1 cut(s) 763
PleI GAGTC 2 cut(s) 48, 437
PpsI GAGTC 2 cut(s) 48, 437
PscI ACATGT 2 cut(s) 115, 648
PspN4I GGNNCC 2 cut(s) 100, 251
PsuI RGATCY 3 cut(s) 11, 622, 741
PvuI CGATCG 1 cut(s) 763
RsaI GTAC 2 cut(s) 240, 468
RsaNI GTAC 2 cut(s) 239, 467
SapI GCTCTTC 1 cut(s) 69
SaqAI TTAA 2 cut(s) 81, 153
SatI GCNGC 1 cut(s) 585
SchI GAGTC 2 cut(s) 49, 437
SfaNI GCATC 1 cut(s) 518
SmlI CTYRAG 2 cut(s) 69, 161
SmoI CTYRAG 2 cut(s) 69, 161
Sse9I AATT 8 cut(s) 139, 180, 200, 208, 244, 265, 272, 289
SsiI CCGC 1 cut(s) 205
SspMI CTAG 1 cut(s) 297
TaaI ACNGT 1 cut(s) 450
TaqI TCGA 3 cut(s) 748, 759, 763
TasI AATT 8 cut(s) 139, 180, 200, 208, 244, 265, 272, 289
TatI WGTACW 1 cut(s) 238
TfiI GAWTC 3 cut(s) 483, 604, 677
Tru1I TTAA 2 cut(s) 81, 153
Tru9I TTAA 2 cut(s) 81, 153
TseFI GTSAC 4 cut(s) 23, 365, 450, 595
TseI GCWGC 1 cut(s) 584
Tsp45I GTSAC 4 cut(s) 23, 365, 450, 595
TspDTI ATGAA 3 cut(s) 20, 146, 475
TspGWI ACGGA 2 cut(s) 449, 733
XapI RAATTY 2 cut(s) 139, 208
XceI RCATGY 2 cut(s) 119, 652
XspI CTAG 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.