FvH4_3g30760

Shugoshin C terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0009270
Physical Location & Seq
Reverse (-)
24165162 .. 24169167
4006 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g30760.t2

Sequence Viewer

Length: 1218 bp
ATGAGAGCCGCGAGAATGGCCACAAGATCCTCTGTCAACACCACAATGCGGCCGAAGCTCGCTGACGTCACCAACTTGCCGGCGCCTCCTTCGAGAAATGAAAACCTCTCCGAAACTTCCATGGGTGACAAGGACCGTGTTGAACAGCTCACCAGGGAAAGAATGGCTCTGATTAAACTCGTCACCGAAAGAAATAAAATTATTGAATTGAGTGGAGCCGAGTTACAGAAGTTGCGCGTTGGCGTTCAGAAATTGCAGCTACAGAATTGGAATCTTGCTAGATCGAACAGCCAGATGTTAGCGGAGCTTAATTCAAAAAGAGATACGGTAAAAACACTACAACATGAACTTTTATGCAAGGAGGCCTTACTTAAGGCAAAGAATTCTGAAATGCAGGAAAAATCAGAGATGAAATGTCAAAATGTTCGATCTAAGTTCAAGGAACCAGATGAGGTAACTTTGCATGAAGCAGATAATGATGACAAACCACGTATTGGCAACAAGAGGCGAGCAACAAGAAGTCAATCTCTTGGTGCTTCTGCTAAATGCCAAAAGGTAGAACAGTCTCTCACTCTACAGGGAAAAGAAGAGATAAATTGTCAAACTACTGGCCATCAGCTCATGGAAGGAGAGAAGCCAGCTTTGCTGGAAGCTACTAAGGATGAAAAACCTTGTGTTGGCAACAAAAGGCGTGCTACAAGAAGTCAATCTATGGGCTCTTCTACTACAGGTCAAAACAATGAACAGAAAGATAAGGTTGAAAATAAAAGGCGGAGTTTGAGGAGGCAGTCTTCTAGATTTAAATCTGACGAGGAAGAACAGACAGAAACTTTGTTTGAGATAGAAGAAGCCAAATTTCCAGAAAATCGAATGCTTGAGGTTGATCCCATTCCCTCCATTTCATCTACCAAGCAAGAAGAGAAAGAAGATAGCTGGGCTGCGAAGAGTGAAGGAGTATCACAAAGATCTTCAATTGGTAGAAGACCATTGCGCAAGGCAGTTAAGAAGGATACCGAATTTCCAGAAAAATTAATTCCTAATTGTGATCCCACTCCTTTAATTTTATCTACCAATAAAGAGAAAGCAGAAATCAGTGCCCCGAAGAGTGAAGGAGTATCGCAAAGATGTTCAGTTGGAAGACCCATGCGGAAAGCTGTTGAGAAGGTTCAGTCCTACAAAGAACCTCCACTCCATGTCAAAATGCGTAGAGCAGAGTGA

Protein Analysis

406

Amino Acids

45.95

Weight (kDa)

9.5

Isoelectric Point (pI)

63.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Shugoshin_C PF07557 366 - 390 5.5e-09 Shugoshin C terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 69
Acc16I TGCGCA 1 cut(s) 992
AccB1I GGYRCC 1 cut(s) 82
AccB7I CCANNNNNTGG 1 cut(s) 494
AccII CGCG 2 cut(s) 11, 237
AciI CCGC 5 cut(s) 9, 49, 302, 772, 1147
AclWI GGATC 3 cut(s) 21, 878, 1040
AcoI YGGCCR 3 cut(s) 18, 50, 610
AcsI RAATTY 3 cut(s) 382, 854, 1016
AcyI GRCGYC 2 cut(s) 66, 83
AfiI CCNNNNNNNGG 3 cut(s) 48, 494, 677
AflII CTTAAG 1 cut(s) 371
AgsI TTSAA 6 cut(s) 143, 206, 315, 439, 761, 972
AjnI CCWGG 1 cut(s) 152
AloI GAACNNNNNNTCC 2 cut(s) 1173, 1205
AluBI AGCT 9 cut(s) 58, 148, 259, 307, 619, 641, 653, 933, 1154
AluI AGCT 9 cut(s) 58, 148, 259, 307, 619, 641, 653, 933, 1154
Alw26I GTCTC 1 cut(s) 570
AlwI GGATC 3 cut(s) 21, 878, 1040
AoxI GGCC 4 cut(s) 18, 50, 363, 610
ApeKI GCWGC 2 cut(s) 256, 938
ApoI RAATTY 3 cut(s) 382, 854, 1016
ArsI GACNNNNNNTTYG 2 cut(s) 814, 846
AseI ATTAAT 1 cut(s) 1031
AspLEI GCGC 3 cut(s) 85, 237, 993
AspS9I GGNCC 1 cut(s) 133
AsuHPI GGTGA 4 cut(s) 61, 137, 142, 175
AvaII GGWCC 1 cut(s) 133
BaeGI GKGCMC 1 cut(s) 1099
BalI TGGCCA 2 cut(s) 20, 612
BanI GGYRCC 1 cut(s) 82
BanII GRGCYC 1 cut(s) 719
BbsI GAAGAC 3 cut(s) 783, 988, 1144
BbvI GCAGC 2 cut(s) 268, 925
BccI CCATC 1 cut(s) 621
BciT130I CCWGG 1 cut(s) 154
BciVI GTATCC 1 cut(s) 1003
BcoDI GTCTC 1 cut(s) 570
BfaI CTAG 2 cut(s) 279, 795
BfmI CTRYAG 3 cut(s) 260, 575, 726
BfoI RGCGCY 1 cut(s) 86
BfrI CTTAAG 1 cut(s) 371
BfuI GTATCC 1 cut(s) 1003
BglII AGATCT 1 cut(s) 965
BisI GCNGC 4 cut(s) 9, 50, 257, 939
BlsI GCNGC 4 cut(s) 10, 51, 258, 940
Bme1390I CCNGG 1 cut(s) 154
Bme18I GGWCC 1 cut(s) 133
BmgT120I GGNCC 1 cut(s) 133
BmiI GGNNCC 3 cut(s) 84, 217, 444
BmrFI CCNGG 1 cut(s) 154
BpiI GAAGAC 3 cut(s) 783, 988, 1144
BpuEI CTTGAG 1 cut(s) 896
BsaAI YACGTR 1 cut(s) 491
BsaBI GATNNNNATC 1 cut(s) 802
BsaHI GRCGYC 2 cut(s) 66, 83
BsaJI CCNNGG 2 cut(s) 120, 153
BsaXI ACNNNNNCTCC 2 cut(s) 1173, 1203
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 494, 677
Bse118I RCCGGY 1 cut(s) 79
Bse1I ACTGG 1 cut(s) 613
Bse3DI GCAATG 1 cut(s) 986
Bse8I GATNNNNATC 1 cut(s) 802
BseBI CCWGG 1 cut(s) 154
BseDI CCNNGG 2 cut(s) 120, 153
BseGI GGATG 1 cut(s) 667
BseJI GATNNNNATC 1 cut(s) 802
BseLI CCNNNNNNNGG 3 cut(s) 48, 494, 677
BseMI GCAATG 1 cut(s) 986
BseNI ACTGG 1 cut(s) 613
BseRI GAGGAG 1 cut(s) 796
BseSI GKGCMC 1 cut(s) 1099
BseX3I CGGCCG 1 cut(s) 50
BseXI GCAGC 2 cut(s) 268, 925
BseYI CCCAGC 1 cut(s) 933
Bsh1236I CGCG 2 cut(s) 11, 237
Bsh1285I CGRYCG 1 cut(s) 53
BshFI GGCC 4 cut(s) 20, 52, 365, 612
BshNI GGYRCC 1 cut(s) 82
BsiEI CGRYCG 1 cut(s) 53
BsiSI CCGG 1 cut(s) 80
BslI CCNNNNNNNGG 3 cut(s) 48, 494, 677
BsmAI GTCTC 1 cut(s) 570
BsmI GAATGC 1 cut(s) 876
BsnI GGCC 4 cut(s) 20, 52, 365, 612
Bsp1286I GDGCHC 2 cut(s) 719, 1099
Bsp143I GATC 6 cut(s) 26, 281, 428, 883, 965, 1045
Bsp19I CCATGG 1 cut(s) 120
BspACI CCGC 5 cut(s) 9, 49, 302, 772, 1147
BspANI GGCC 4 cut(s) 20, 52, 365, 612
BspFNI CGCG 2 cut(s) 11, 237
BspLI GGNNCC 3 cut(s) 84, 217, 444
BspPI GGATC 3 cut(s) 21, 878, 1040
BspQI GCTCTTC 1 cut(s) 724
BspT107I GGYRCC 1 cut(s) 82
BspTI CTTAAG 1 cut(s) 371
BsrDI GCAATG 1 cut(s) 986
BsrFI RCCGGY 1 cut(s) 79
BsrI ACTGG 1 cut(s) 613
BssAI RCCGGY 1 cut(s) 79
BssECI CCNNGG 2 cut(s) 120, 153
BssMI GATC 6 cut(s) 26, 281, 428, 883, 965, 1045
BssNI GRCGYC 2 cut(s) 66, 83
BssT1I CCWWGG 1 cut(s) 120
Bst2UI CCWGG 1 cut(s) 154
Bst4CI ACNGT 3 cut(s) 137, 328, 564
Bst6I CTCTTC 5 cut(s) 582, 724, 912, 938, 1097
BstACI GRCGYC 2 cut(s) 66, 83
BstAFI CTTAAG 1 cut(s) 371
BstBAI YACGTR 1 cut(s) 491
BstC8I GCNNGC 5 cut(s) 60, 81, 510, 639, 693
BstDEI CTNAG 2 cut(s) 432, 657
BstDSI CCRYGG 1 cut(s) 120
BstF5I GGATG 1 cut(s) 667
BstFNI CGCG 2 cut(s) 11, 237
BstH2I RGCGCY 1 cut(s) 86
BstHHI GCGC 3 cut(s) 85, 237, 993
BstKTI GATC 6 cut(s) 29, 284, 431, 886, 968, 1048
BstMAI GTCTC 1 cut(s) 570
BstMBI GATC 6 cut(s) 26, 281, 428, 883, 965, 1045
BstMCI CGRYCG 1 cut(s) 53
BstMWI GCNNNNNNNGC 3 cut(s) 17, 55, 643
BstNI CCWGG 1 cut(s) 154
BstSCI CCNGG 1 cut(s) 152
BstSFI CTRYAG 3 cut(s) 260, 575, 726
BstSLI GKGCMC 1 cut(s) 1099
BstUI CGCG 2 cut(s) 11, 237
BstV1I GCAGC 2 cut(s) 268, 925
BstV2I GAAGAC 3 cut(s) 783, 988, 1144
BstX2I RGATCY 2 cut(s) 26, 965
BstYI RGATCY 2 cut(s) 26, 965
BstZI CGGCCG 1 cut(s) 50
BsuI GTATCC 1 cut(s) 1003
BsuRI GGCC 4 cut(s) 20, 52, 365, 612
BtgI CCRYGG 1 cut(s) 120
BtsCI GGATG 1 cut(s) 667
BtsIMutI CAGTG 1 cut(s) 1099
Cac8I GCNNGC 5 cut(s) 60, 81, 510, 639, 693
CfoI GCGC 3 cut(s) 85, 237, 993
Cfr10I RCCGGY 1 cut(s) 79
Cfr13I GGNCC 1 cut(s) 133
CviAII CATG 6 cut(s) 121, 344, 464, 622, 1144, 1193
DdeI CTNAG 2 cut(s) 432, 657
DinI GGCGCC 1 cut(s) 84
DpnI GATC 6 cut(s) 28, 283, 430, 885, 967, 1047
DpnII GATC 6 cut(s) 26, 281, 428, 883, 965, 1045
DraI TTTAAA 1 cut(s) 802
EaeI YGGCCR 3 cut(s) 18, 50, 610
EagI CGGCCG 1 cut(s) 50
Eam1104I CTCTTC 5 cut(s) 582, 724, 912, 938, 1097
EarI CTCTTC 5 cut(s) 582, 724, 912, 938, 1097
EciI GGCGGA 1 cut(s) 787
EclXI CGGCCG 1 cut(s) 50
Eco130I CCWWGG 1 cut(s) 120
Eco147I AGGCCT 1 cut(s) 365
Eco24I GRGCYC 1 cut(s) 719
Eco47I GGWCC 1 cut(s) 133
Eco52I CGGCCG 1 cut(s) 50
EcoRI GAATTC 1 cut(s) 382
EcoRII CCWGG 1 cut(s) 152
EcoT14I CCWWGG 1 cut(s) 120
EcoT38I GRGCYC 1 cut(s) 719
EgeI GGCGCC 1 cut(s) 84
EheI GGCGCC 1 cut(s) 84
ErhI CCWWGG 1 cut(s) 120
FaeI CATG 6 cut(s) 124, 347, 467, 625, 1147, 1196
FaiI YATR 8 cut(s) 122, 345, 355, 465, 623, 713, 1145, 1194
FalI AAGNNNNNCTT 2 cut(s) 350, 382
FatI CATG 6 cut(s) 120, 343, 463, 621, 1143, 1192
Fnu4HI GCNGC 4 cut(s) 9, 50, 257, 939
FokI GGATG 1 cut(s) 674
FriOI GRGCYC 1 cut(s) 719
Fsp4HI GCNGC 4 cut(s) 9, 50, 257, 939
FspBI CTAG 2 cut(s) 279, 795
FspI TGCGCA 1 cut(s) 992
GlaI GCGC 3 cut(s) 84, 236, 992
GluI GCNGC 4 cut(s) 9, 50, 257, 939
GsaI CCCAGC 1 cut(s) 937
HaeII RGCGCY 1 cut(s) 86
HaeIII GGCC 4 cut(s) 20, 52, 365, 612
HapII CCGG 1 cut(s) 80
HhaI GCGC 3 cut(s) 85, 237, 993
Hin1I GRCGYC 2 cut(s) 66, 83
Hin1II CATG 6 cut(s) 124, 347, 467, 625, 1147, 1196
Hin6I GCGC 3 cut(s) 83, 235, 991
HinP1I GCGC 3 cut(s) 83, 235, 991
HincII GTYRAC 1 cut(s) 37
HindII GTYRAC 1 cut(s) 37
HinfI GANTC 1 cut(s) 271
HpaII CCGG 1 cut(s) 80
HphI GGTGA 4 cut(s) 61, 137, 142, 175
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 6 cut(s) 112, 171, 249, 388, 406, 808
Hpy188III TCNNGA 4 cut(s) 93, 795, 860, 1022
Hpy8I GTNNAC 1 cut(s) 37
HpyAV CCTTC 6 cut(s) 99, 620, 944, 1000, 1103, 1156
HpyCH4III ACNGT 3 cut(s) 137, 328, 564
HpyCH4IV ACGT 2 cut(s) 66, 490
HpyCH4V TGCA 4 cut(s) 256, 357, 394, 463
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 55, 643
HpyF3I CTNAG 2 cut(s) 432, 657
HpySE526I ACGT 2 cut(s) 66, 490
Hsp92I GRCGYC 2 cut(s) 66, 83
Hsp92II CATG 6 cut(s) 124, 347, 467, 625, 1147, 1196
HspAI GCGC 3 cut(s) 83, 235, 991
KasI GGCGCC 1 cut(s) 82
KroI GCCGGC 1 cut(s) 79
KroNI GCCGGC 1 cut(s) 81
Kzo9I GATC 6 cut(s) 26, 281, 428, 883, 965, 1045
LguI GCTCTTC 1 cut(s) 724
LmnI GCTCC 2 cut(s) 215, 304
Lsp1109I GCAGC 2 cut(s) 268, 925
MaeI CTAG 2 cut(s) 279, 795
MaeII ACGT 2 cut(s) 66, 490
MaeIII GTNAC 5 cut(s) 67, 125, 181, 222, 454
MalI GATC 6 cut(s) 28, 283, 430, 885, 967, 1047
MboI GATC 6 cut(s) 26, 281, 428, 883, 965, 1045
MfeI CAATTG 1 cut(s) 972
MflI RGATCY 2 cut(s) 26, 965
MhlI GDGCHC 2 cut(s) 719, 1099
MlsI TGGCCA 2 cut(s) 20, 612
MluNI TGGCCA 2 cut(s) 20, 612
Mly113I GGCGCC 1 cut(s) 83
MmeI TCCRAC 1 cut(s) 1114
Mox20I TGGCCA 2 cut(s) 20, 612
MroNI GCCGGC 1 cut(s) 79
MscI TGGCCA 2 cut(s) 20, 612
MseI TTAA 7 cut(s) 174, 309, 372, 801, 1002, 1031, 1058
MslI CAYNNNNRTG 1 cut(s) 44
Msp20I TGGCCA 2 cut(s) 20, 612
MspCI CTTAAG 1 cut(s) 371
MspI CCGG 1 cut(s) 80
MspR9I CCNGG 1 cut(s) 154
MunI CAATTG 1 cut(s) 972
Mva1269I GAATGC 1 cut(s) 876
MvaI CCWGG 1 cut(s) 154
MvnI CGCG 2 cut(s) 11, 237
MwoI GCNNNNNNNGC 3 cut(s) 17, 55, 643
NaeI GCCGGC 1 cut(s) 81
NarI GGCGCC 1 cut(s) 83
NcoI CCATGG 1 cut(s) 120
NdeII GATC 6 cut(s) 26, 281, 428, 883, 965, 1045
NgoMIV GCCGGC 1 cut(s) 79
NlaIII CATG 6 cut(s) 124, 347, 467, 625, 1147, 1196
NlaIV GGNNCC 3 cut(s) 84, 217, 444
NmeAIII GCCGAG 1 cut(s) 244
NmuCI GTSAC 3 cut(s) 67, 125, 181
NsbI TGCGCA 1 cut(s) 992
PceI AGGCCT 1 cut(s) 365
PciSI GCTCTTC 1 cut(s) 724
PctI GAATGC 1 cut(s) 876
PdiI GCCGGC 1 cut(s) 81
PfeI GAWTC 1 cut(s) 271
PflMI CCANNNNNTGG 1 cut(s) 494
PkrI GCNGC 4 cut(s) 10, 51, 258, 940
PluTI GGCGCC 1 cut(s) 86
Ppu21I YACGTR 1 cut(s) 491
PshBI ATTAAT 1 cut(s) 1031
Psp6I CCWGG 1 cut(s) 152
PspFI CCCAGC 1 cut(s) 933
PspGI CCWGG 1 cut(s) 152
PspN4I GGNNCC 3 cut(s) 84, 217, 444
PspPI GGNCC 1 cut(s) 133
PsuI RGATCY 2 cut(s) 26, 965
RseI CAYNNNNRTG 1 cut(s) 44
SapI GCTCTTC 1 cut(s) 724
SaqAI TTAA 7 cut(s) 174, 309, 372, 801, 1002, 1031, 1058
SatI GCNGC 4 cut(s) 9, 50, 257, 939
Sau3AI GATC 6 cut(s) 26, 281, 428, 883, 965, 1045
Sau96I GGNCC 1 cut(s) 133
ScrFI CCNGG 1 cut(s) 154
SduI GDGCHC 2 cut(s) 719, 1099
SfcI CTRYAG 3 cut(s) 260, 575, 726
SfoI GGCGCC 1 cut(s) 84
SinI GGWCC 1 cut(s) 133
SmiI ATTTAAAT 1 cut(s) 802
SmiMI CAYNNNNRTG 1 cut(s) 44
SmlI CTYRAG 2 cut(s) 371, 875
SmoI CTYRAG 2 cut(s) 371, 875
SseBI AGGCCT 1 cut(s) 365
SsiI CCGC 5 cut(s) 9, 49, 302, 772, 1147
SspDI GGCGCC 1 cut(s) 82
SspMI CTAG 2 cut(s) 279, 795
StuI AGGCCT 1 cut(s) 365
StyD4I CCNGG 1 cut(s) 152
StyI CCWWGG 1 cut(s) 120
SwaI ATTTAAAT 1 cut(s) 802
TaaI ACNGT 3 cut(s) 137, 328, 564
TaiI ACGT 2 cut(s) 69, 493
TaqI TCGA 4 cut(s) 92, 284, 427, 868
TauI GCSGC 2 cut(s) 11, 52
TfiI GAWTC 1 cut(s) 271
Tru1I TTAA 7 cut(s) 174, 309, 372, 801, 1002, 1031, 1058
Tru9I TTAA 7 cut(s) 174, 309, 372, 801, 1002, 1031, 1058
TscAI CASTG 1 cut(s) 1099
TseFI GTSAC 3 cut(s) 67, 125, 181
TseI GCWGC 2 cut(s) 256, 938
Tsp45I GTSAC 3 cut(s) 67, 125, 181
TspDTI ATGAA 7 cut(s) 114, 360, 425, 480, 678, 756, 891
TspRI CASTG 1 cut(s) 1099
Van91I CCANNNNNTGG 1 cut(s) 494
Vha464I CTTAAG 1 cut(s) 371
VpaK11BI GGWCC 1 cut(s) 133
VspI ATTAAT 1 cut(s) 1031
XapI RAATTY 3 cut(s) 382, 854, 1016
XbaI TCTAGA 1 cut(s) 794
XcmI CCANNNNNNNNNTGG 1 cut(s) 160
XspI CTAG 2 cut(s) 279, 795
ZraI GACGTC 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.