AT3G50340

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014002
Physical Location & Seq
Reverse (-)
18664838 .. 18666691
1854 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G50340.1

Sequence Viewer

Length: 1212 bp
ATGGTCGACGTTGATCGGAGAATGACCGGTCTCAGACCAGCTCACGCCGCTGGTCTCCGTCGTCTCTCTGCTCGCGCCGCCGCACCAACAACACCAACGGTTAGAAACAGTCTCGTCTCTTTCTCTTCTCTCGCCGATCAAGTAATCTCACACTTACACACTTCAAGGATCCAAGTCCAACCGGGTCTAACCGACTCCGAATTCGCTAGAGCAGAAGCAGAGTTTGCATTCGCTTTTCCACCGGACCTCCGTGCTGTTCTCACCGCCGGTTTACCAGTCGGCGCCGGATTTCCAGACTGGCGTTCTCCCGGAGCTCGTCTTCATCTCAGAGCTATGATCGATCTTCCTATCGCCGCCGTGTCGTTTCAGATCGCGAGAAACACTCTCTGGAGCAAATCTTGGGGTTTAAGACCATCCGACCCGGAAAAAGCTTTACGGGTCGCGAGAAATGCTCTCAAAAGAGCTCCTTTGATGATACCAATCTTTGATCATTGCTATATTCCTTGTAACCCGTCTTTAGCGGGTAACCCGGTTTTTTATATTGACGAGACCCGGATTTTCTGTTGCGGGTCGGATCTTTCTGATTTCTTCGAGCGTGAGTCTGTGTTTAGAGGGTCTGACACGTGTCCGGTTGTTTTAACGAAGCAGCGTTCGGTGAGTGAGAAATCAGCCGGGTCGTCGTCGTCTTCTTCATCAAATTTTTCGAGAATGAGTTTAGATTCGGGTCGGGTTCACGGGTCGAGTACACCGAGATGGGTTGAGTTTTGGAGTGACGCGGCGGTTGATCGTCGGAGGAGAAACTCTGCTTCTTCTATGTCGTCGTCACACTCTTCATCGCCGGAGAGATATTTAGATTTACCAAGATCCGAGACACCGAAATGGGTCGACGATTACGTGAACCGGATCGGGTCGGTTTTAAGAGGAGGCGGGTGGAGCGAATCCGACGTTGATGATATTGTACACGTGTCAGCATCTGGTTTTTTTGAGGGTGAAATGGTAATTTTGGATAATCAAGCCGTTCTTGATGCATTGCTTTTGAAAGCGGGTCGGTTCTCGGAGTCGCTCCGAAAAGCTGGATGGAGCTCCGAAGAAGTATCGGATGCACTTGGTTTTGATTTTCGACCGGAGAAGGAGAAGAAACCGGTGAAGAAGCTATCTCCTGAACTTGTCCAACGAATCGGGAAACTTGCTGAGTCGGTTTCTCGGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

403

Amino Acids

44.3

Weight (kDa)

8.98

Isoelectric Point (pI)

54.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014002)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50340 AT5G67020
fragaria_vesca FvH4_1g16070
malus_domestica MD02G1172200.v1.1 MD15G1284400.v1.1
prunus_persica Prupe.7G133200_v2.0.a1
pyrus_communis pycom02g13880 pycom15g24700 pycom15g24710
rosa_chinensis RchiOBHm_Chr2g0105691
rosa_laevigata RLG00000017449
rosa_rugosa Rorug02G0129200
rosa_samantha Rh2AG180900 Rh2BG190000 Rh2CG185100 Rh2DG186900
rosa_wichuraiana Rw2G014140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 281
AccI GTMKAC 2 cut(s) 6, 885
AccII CGCG 4 cut(s) 75, 374, 443, 776
AclWI GGATC 5 cut(s) 163, 176, 582, 858, 911
AcsI RAATTY 2 cut(s) 200, 697
AcvI CACGTG 2 cut(s) 624, 964
AcyI GRCGYC 1 cut(s) 282
AfaI GTAC 2 cut(s) 745, 960
AflIII ACRYGT 4 cut(s) 621, 623, 961, 963
AgeI ACCGGT 2 cut(s) 26, 1141
AgsI TTSAA 2 cut(s) 165, 1039
AluBI AGCT 8 cut(s) 41, 314, 332, 431, 464, 1073, 1083, 1153
AluI AGCT 8 cut(s) 41, 314, 332, 431, 464, 1073, 1083, 1153
Alw21I GWGCWC 3 cut(s) 316, 466, 1085
Alw26I GTCTC 7 cut(s) 35, 59, 68, 116, 121, 542, 863
AlwI GGATC 5 cut(s) 163, 176, 582, 858, 911
AlwNI CAGNNNCTG 1 cut(s) 974
ApeKI GCWGC 1 cut(s) 646
ApoI RAATTY 2 cut(s) 200, 697
ArsI GACNNNNNNTTYG 2 cut(s) 185, 217
AsiGI ACCGGT 2 cut(s) 26, 1141
AspLEI GCGC 2 cut(s) 77, 284
AspS9I GGNCC 1 cut(s) 244
AsuC2I CCSGG 6 cut(s) 183, 309, 422, 530, 553, 673
AsuHPI GGTGA 4 cut(s) 253, 667, 1001, 1156
AvaII GGWCC 1 cut(s) 244
BamHI GGATCC 1 cut(s) 168
BanI GGYRCC 1 cut(s) 281
BanII GRGCYC 3 cut(s) 316, 466, 1085
BbrPI CACGTG 2 cut(s) 624, 964
BbsI GAAGAC 2 cut(s) 311, 678
Bbv12I GWGCWC 3 cut(s) 316, 466, 1085
BbvI GCAGC 1 cut(s) 658
BccI CCATC 3 cut(s) 421, 747, 1071
BceAI ACGGC 2 cut(s) 341, 1001
BclI TGATCA 1 cut(s) 487
BcnI CCSGG 6 cut(s) 183, 309, 422, 530, 553, 673
BcoDI GTCTC 7 cut(s) 35, 59, 68, 116, 121, 542, 863
BfaI CTAG 1 cut(s) 207
BfoI RGCGCY 1 cut(s) 285
BisI GCNGC 6 cut(s) 48, 78, 81, 354, 647, 777
BlsI GCNGC 6 cut(s) 49, 79, 82, 355, 648, 778
Bme1390I CCNGG 6 cut(s) 183, 309, 422, 530, 553, 673
Bme18I GGWCC 1 cut(s) 244
BmgT120I GGNCC 1 cut(s) 244
BmiI GGNNCC 2 cut(s) 170, 283
BmrFI CCNGG 6 cut(s) 183, 309, 422, 530, 553, 673
BmsI GCATC 3 cut(s) 980, 1015, 1090
BoxI GACNNNNGTC 1 cut(s) 624
BpiI GAAGAC 2 cut(s) 311, 678
BplI GAGNNNNNCTC 4 cut(s) 367, 399, 436, 468
BpmI CTGGAG 1 cut(s) 409
BpuMI CCSGG 6 cut(s) 183, 309, 422, 530, 553, 673
Bsa29I ATCGAT 1 cut(s) 339
BsaAI YACGTR 3 cut(s) 624, 895, 964
BsaBI GATNNNNATC 1 cut(s) 479
BsaHI GRCGYC 1 cut(s) 282
BsaI GGTCTC 3 cut(s) 35, 59, 542
BsaWI WCCGGW 6 cut(s) 26, 241, 628, 900, 1123, 1141
BsaXI ACNNNNNCTCC 2 cut(s) 231, 261
Bse118I RCCGGY 3 cut(s) 26, 266, 1141
Bse1I ACTGG 2 cut(s) 275, 302
Bse3DI GCAATG 2 cut(s) 490, 1028
Bse8I GATNNNNATC 1 cut(s) 479
BseCI ATCGAT 1 cut(s) 339
BseGI GGATG 3 cut(s) 413, 1082, 1105
BseJI GATNNNNATC 1 cut(s) 479
BseMI GCAATG 2 cut(s) 490, 1028
BseMII CTCAG 3 cut(s) 46, 340, 1182
BseNI ACTGG 2 cut(s) 275, 302
BseRI GAGGAG 2 cut(s) 808, 936
BseXI GCAGC 1 cut(s) 658
Bsh1236I CGCG 4 cut(s) 75, 374, 443, 776
Bsh1285I CGRYCG 1 cut(s) 1124
BshNI GGYRCC 1 cut(s) 281
BshTI ACCGGT 2 cut(s) 26, 1141
BshVI ATCGAT 1 cut(s) 339
BsiEI CGRYCG 1 cut(s) 1124
BsiHKAI GWGCWC 3 cut(s) 316, 466, 1085
BsmAI GTCTC 7 cut(s) 35, 59, 68, 116, 121, 542, 863
BsmBI CGTCTC 2 cut(s) 68, 121
BsmI GAATGC 1 cut(s) 227
Bso31I GGTCTC 3 cut(s) 35, 59, 542
Bsp1286I GDGCHC 3 cut(s) 316, 466, 1085
Bsp1407I TGTACA 1 cut(s) 958
Bsp68I TCGCGA 2 cut(s) 374, 443
BspCNI CTCAG 3 cut(s) 45, 339, 1183
BspDI ATCGAT 1 cut(s) 339
BspFNI CGCG 4 cut(s) 75, 374, 443, 776
BspHI TCATGA 1 cut(s) 1208
BspLI GGNNCC 2 cut(s) 170, 283
BspPI GGATC 5 cut(s) 163, 176, 582, 858, 911
BspT107I GGYRCC 1 cut(s) 281
BspTNI GGTCTC 3 cut(s) 35, 59, 542
BsrDI GCAATG 2 cut(s) 490, 1028
BsrFI RCCGGY 3 cut(s) 26, 266, 1141
BsrGI TGTACA 1 cut(s) 958
BsrI ACTGG 2 cut(s) 275, 302
BssAI RCCGGY 3 cut(s) 26, 266, 1141
BssNI GRCGYC 1 cut(s) 282
Bst4CI ACNGT 2 cut(s) 100, 110
Bst6I CTCTTC 2 cut(s) 130, 835
BstACI GRCGYC 1 cut(s) 282
BstAPI GCANNNNNTGC 1 cut(s) 224
BstAUI TGTACA 1 cut(s) 958
BstBAI YACGTR 3 cut(s) 624, 895, 964
BstC8I GCNNGC 1 cut(s) 73
BstDEI CTNAG 3 cut(s) 32, 326, 1191
BstEII GGTNACC 1 cut(s) 524
BstF5I GGATG 3 cut(s) 413, 1082, 1105
BstFNI CGCG 4 cut(s) 75, 374, 443, 776
BstH2I RGCGCY 1 cut(s) 285
BstHHI GCGC 2 cut(s) 77, 284
BstMAI GTCTC 7 cut(s) 35, 59, 68, 116, 121, 542, 863
BstMCI CGRYCG 1 cut(s) 1124
BstMWI GCNNNNNNNGC 5 cut(s) 47, 77, 224, 449, 933
BstPAI GACNNNNGTC 1 cut(s) 624
BstPI GGTNACC 1 cut(s) 524
BstSCI CCNGG 6 cut(s) 181, 307, 420, 528, 551, 671
BstUI CGCG 4 cut(s) 75, 374, 443, 776
BstV1I GCAGC 1 cut(s) 658
BstV2I GAAGAC 2 cut(s) 311, 678
BstX2I RGATCY 3 cut(s) 168, 574, 863
BstYI RGATCY 3 cut(s) 168, 574, 863
Bsu15I ATCGAT 1 cut(s) 339
BsuTUI ATCGAT 1 cut(s) 339
BtgZI GCGATG 1 cut(s) 819
BtsCI GGATG 3 cut(s) 413, 1082, 1105
BtuMI TCGCGA 2 cut(s) 374, 443
Cac8I GCNNGC 1 cut(s) 73
CaiI CAGNNNCTG 1 cut(s) 974
CciI TCATGA 1 cut(s) 1208
CfoI GCGC 2 cut(s) 77, 284
Cfr10I RCCGGY 3 cut(s) 26, 266, 1141
Cfr13I GGNCC 1 cut(s) 244
ClaI ATCGAT 1 cut(s) 339
CseI GACGC 1 cut(s) 782
Csp6I GTAC 2 cut(s) 744, 959
CspAI ACCGGT 2 cut(s) 26, 1141
CviAII CATG 1 cut(s) 1209
CviQI GTAC 2 cut(s) 744, 959
DdeI CTNAG 3 cut(s) 32, 326, 1191
DinI GGCGCC 1 cut(s) 283
Eam1104I CTCTTC 2 cut(s) 130, 835
EarI CTCTTC 2 cut(s) 130, 835
Ecl136II GAGCTC 3 cut(s) 314, 464, 1083
Eco24I GRGCYC 3 cut(s) 316, 466, 1085
Eco31I GGTCTC 3 cut(s) 35, 59, 542
Eco47I GGWCC 1 cut(s) 244
Eco53kI GAGCTC 3 cut(s) 314, 464, 1083
Eco72I CACGTG 2 cut(s) 624, 964
Eco91I GGTNACC 1 cut(s) 524
EcoICRI GAGCTC 3 cut(s) 314, 464, 1083
EcoO65I GGTNACC 1 cut(s) 524
EcoRI GAATTC 1 cut(s) 200
EcoT22I ATGCAT 1 cut(s) 1030
EcoT38I GRGCYC 3 cut(s) 316, 466, 1085
EgeI GGCGCC 1 cut(s) 283
EheI GGCGCC 1 cut(s) 283
Esp3I CGTCTC 2 cut(s) 68, 121
FaeI CATG 1 cut(s) 1212
FaiI YATR 5 cut(s) 335, 498, 540, 815, 1210
FalI AAGNNNNNCTT 4 cut(s) 451, 483, 1005, 1037
FatI CATG 1 cut(s) 1208
FauI CCCGC 4 cut(s) 514, 560, 920, 1036
FbaI TGATCA 1 cut(s) 487
FblI GTMKAC 2 cut(s) 6, 885
Fnu4HI GCNGC 6 cut(s) 48, 78, 81, 354, 647, 777
FokI GGATG 3 cut(s) 400, 1089, 1112
FriOI GRGCYC 3 cut(s) 316, 466, 1085
Fsp4HI GCNGC 6 cut(s) 48, 78, 81, 354, 647, 777
FspBI CTAG 1 cut(s) 207
GlaI GCGC 2 cut(s) 76, 283
GluI GCNGC 6 cut(s) 48, 78, 81, 354, 647, 777
GsuI CTGGAG 1 cut(s) 409
HaeII RGCGCY 1 cut(s) 285
HgaI GACGC 1 cut(s) 782
HhaI GCGC 2 cut(s) 77, 284
Hin1I GRCGYC 1 cut(s) 282
Hin1II CATG 1 cut(s) 1212
Hin6I GCGC 2 cut(s) 75, 282
HinP1I GCGC 2 cut(s) 75, 282
HincII GTYRAC 2 cut(s) 7, 886
HindII GTYRAC 2 cut(s) 7, 886
HindIII AAGCTT 1 cut(s) 429
HinfI GANTC 7 cut(s) 194, 599, 719, 938, 1058, 1176, 1193
HphI GGTGA 4 cut(s) 253, 667, 1001, 1156
Hpy166II GTNNAC 7 cut(s) 7, 272, 733, 746, 886, 898, 961
Hpy188III TCNNGA 9 cut(s) 293, 373, 388, 442, 705, 1022, 1160, 1180, 1209
Hpy8I GTNNAC 7 cut(s) 7, 272, 733, 746, 886, 898, 961
Hpy99I CGWCG 8 cut(s) 11, 63, 682, 685, 792, 823, 890, 947
HpyAV CCTTC 1 cut(s) 1123
HpyCH4III ACNGT 2 cut(s) 100, 110
HpyCH4IV ACGT 5 cut(s) 9, 623, 894, 945, 963
HpyCH4V TGCA 3 cut(s) 227, 1028, 1103
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 77, 224, 449, 933
HpyF3I CTNAG 3 cut(s) 32, 326, 1191
HpySE526I ACGT 5 cut(s) 9, 623, 894, 945, 963
Hsp92I GRCGYC 1 cut(s) 282
Hsp92II CATG 1 cut(s) 1212
HspAI GCGC 2 cut(s) 75, 282
KasI GGCGCC 1 cut(s) 281
Ksp22I TGATCA 1 cut(s) 487
LmnI GCTCC 7 cut(s) 311, 390, 469, 933, 1068, 1080, 1088
Lsp1109I GCAGC 1 cut(s) 658
LweI GCATC 3 cut(s) 980, 1015, 1090
MaeI CTAG 1 cut(s) 207
MaeII ACGT 5 cut(s) 9, 623, 894, 945, 963
MaeIII GTNAC 4 cut(s) 506, 524, 770, 822
MflI RGATCY 3 cut(s) 168, 574, 863
MhlI GDGCHC 3 cut(s) 316, 466, 1085
MluCI AATT 3 cut(s) 200, 697, 999
Mly113I GGCGCC 1 cut(s) 282
MlyI GAGTC 4 cut(s) 188, 608, 1067, 1202
MmeI TCCRAC 6 cut(s) 202, 441, 552, 770, 966, 1195
MnlI CCTC 6 cut(s) 257, 605, 786, 914, 917, 979
Mph1103I ATGCAT 1 cut(s) 1030
MseI TTAA 3 cut(s) 407, 638, 917
MslI CAYNNNNRTG 2 cut(s) 751, 877
MspA1I CMGCKG 1 cut(s) 50
MspR9I CCNGG 6 cut(s) 183, 309, 422, 530, 553, 673
Mva1269I GAATGC 1 cut(s) 227
MvnI CGCG 4 cut(s) 75, 374, 443, 776
MwoI GCNNNNNNNGC 5 cut(s) 47, 77, 224, 449, 933
NarI GGCGCC 1 cut(s) 282
NciI CCSGG 6 cut(s) 183, 309, 422, 530, 553, 673
NlaIII CATG 1 cut(s) 1212
NlaIV GGNNCC 2 cut(s) 170, 283
NmuCI GTSAC 2 cut(s) 770, 822
NruI TCGCGA 2 cut(s) 374, 443
NsiI ATGCAT 1 cut(s) 1030
PagI TCATGA 1 cut(s) 1208
PcsI WCGNNNNNNNCGW 2 cut(s) 746, 891
PctI GAATGC 1 cut(s) 227
PfeI GAWTC 3 cut(s) 719, 938, 1176
PfoI TCCNGGA 1 cut(s) 307
PinAI ACCGGT 2 cut(s) 26, 1141
PkrI GCNGC 6 cut(s) 49, 79, 82, 355, 648, 778
PleI GAGTC 4 cut(s) 188, 607, 1066, 1201
PluTI GGCGCC 1 cut(s) 285
PmaCI CACGTG 2 cut(s) 624, 964
PmlI CACGTG 2 cut(s) 624, 964
PpsI GAGTC 4 cut(s) 188, 607, 1066, 1201
Ppu21I YACGTR 3 cut(s) 624, 895, 964
PshAI GACNNNNGTC 1 cut(s) 624
Psp124BI GAGCTC 3 cut(s) 316, 466, 1085
PspCI CACGTG 2 cut(s) 624, 964
PspEI GGTNACC 1 cut(s) 524
PspN4I GGNNCC 2 cut(s) 170, 283
PspPI GGNCC 1 cut(s) 244
PstNI CAGNNNCTG 1 cut(s) 974
PsuI RGATCY 3 cut(s) 168, 574, 863
RruI TCGCGA 2 cut(s) 374, 443
RsaI GTAC 2 cut(s) 745, 960
RsaNI GTAC 2 cut(s) 744, 959
RseI CAYNNNNRTG 2 cut(s) 751, 877
SacI GAGCTC 3 cut(s) 316, 466, 1085
SalI GTCGAC 2 cut(s) 5, 884
SaqAI TTAA 3 cut(s) 407, 638, 917
SatI GCNGC 6 cut(s) 48, 78, 81, 354, 647, 777
Sau96I GGNCC 1 cut(s) 244
SchI GAGTC 4 cut(s) 188, 608, 1067, 1202
ScrFI CCNGG 6 cut(s) 183, 309, 422, 530, 553, 673
SduI GDGCHC 3 cut(s) 316, 466, 1085
SfaNI GCATC 3 cut(s) 980, 1015, 1090
SfoI GGCGCC 1 cut(s) 283
SinI GGWCC 1 cut(s) 244
SmiMI CAYNNNNRTG 2 cut(s) 751, 877
Sse9I AATT 3 cut(s) 200, 697, 999
SspDI GGCGCC 1 cut(s) 281
SspMI CTAG 1 cut(s) 207
SstI GAGCTC 3 cut(s) 316, 466, 1085
StyD4I CCNGG 6 cut(s) 181, 307, 420, 528, 551, 671
TaaI ACNGT 2 cut(s) 100, 110
TaiI ACGT 5 cut(s) 12, 626, 897, 948, 966
TaqI TCGA 7 cut(s) 6, 339, 591, 704, 740, 885, 1120
TaqII GACCGA 1 cut(s) 1194
TasI AATT 3 cut(s) 200, 697, 999
TatI WGTACW 2 cut(s) 743, 958
TauI GCSGC 5 cut(s) 50, 80, 83, 356, 779
TfiI GAWTC 3 cut(s) 719, 938, 1176
Tru1I TTAA 3 cut(s) 407, 638, 917
Tru9I TTAA 3 cut(s) 407, 638, 917
TseFI GTSAC 2 cut(s) 770, 822
TseI GCWGC 1 cut(s) 646
Tsp45I GTSAC 2 cut(s) 770, 822
TspDTI ATGAA 3 cut(s) 311, 681, 822
TspGWI ACGGA 2 cut(s) 47, 239
VpaK11BI GGWCC 1 cut(s) 244
XapI RAATTY 2 cut(s) 200, 697
XmiI GTMKAC 2 cut(s) 6, 885
XspI CTAG 1 cut(s) 207
Zsp2I ATGCAT 1 cut(s) 1030
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.