MD15G1284400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014002
Physical Location & Seq
Forward (+)
25768569 .. 25769789
1221 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1284400.v1.1.491

Sequence Viewer

Length: 1221 bp
ATGGTCGACGTCGATCGGAGAATGACCGGCCTCAACCCGGGTCACGTAGCCGGCCTTCGCCGTCTCTCCGCCCGAGCCGCCGCCGGCCCACCAACCCCAACAACCGCCGCAAAATCCACTCTTCCCGTACGGAACGGCCTTCTCTCCTTCTCCTCTTTAGCTGACAACGTCATATCCCACCTCAAAAGCTCCGGTTTCCAGGTCCAGCCGGGTCTATCTGAATCCGAGTTCGCCCGGGCTGAAGCGGAGTTCGGCTTCGTATTCCCTCCAGACCTCAGAGCCATTCTCTCAGCCGGGTTGCCTGTTGGCCCCGGTTTTCCCGACTGGAGAGCCGCCGGTGCCCGCCTCGGTCTCAAGGCCTCGCTCGACCTCCCGATCGCTGCGATTTCGTTCCAAATCGCAAGGAACACCCTCTGGTCCAAATCCTGGGGTCTGAGACCGTCCGACCCGGAAAAGGCCTTGCGGGTCGCGAGAACTGCTTTGAAGAGAGCCCCGTTGCTGATACCAATTTTCAACCATTGCTACATTCCGTGCAACCCCTGTTTGGCGGGGAACCCCATCTTCTACGTCGACGAGAATCGGATCTTCTGTTGTGGCTTGGATCTATCCGATTTCTTCGAGCGCGAGTCTCTGTTTCGGAAATCGGAGACCGACCCGTATATCCTTAAGAAGCAAAGGTCAGTGAGCGAGAAATCGGCCGGGTCGTCTTCTAATTTCTCGCGGCGGAGCCTGGACACTGGTTTCGGTTCAGGAACCAAAACCCCTAGATGGGTCGAGTTCTGGAGCGACGCAGCCGTGGATCGTCGCCGGAGGAACTCAAACTCTTCGTCGTCGTCGTGCTCTTCTCCGGAAAGGTTCTTCGATATGCCGTCGAGGTCCGAAATCCCGAAATGGGTGGACGAGTACATTGATCAAATCGGGTCGGCTTTGAGACATGGCGGGTGGAGCGATACGGATATATCGGAGATGGTTTCGGTATCGGCGTCTGGGTTTTTCGAGGGAGAGATGGTTATGTTGGACAACCAAGCGGTACTCGACGCGCTGCTGCTCAAAGCGGATCGGTTCTCGGACTCGCTCCGAAAATCCGGGTGGAGCTCCGAGGAAGTTTCGGAGGCTTTGGGCTTCAATTTTCGACCCGAGAAGGAGAGGAAACCGGCTAAGAAGCTGTCTCCGGAGCTCGTGGAAAAGATAGGGAAACTGGCCGAGTCGGTTTCCCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

407

Amino Acids

44.65

Weight (kDa)

8.97

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014002)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50340 AT5G67020
fragaria_vesca FvH4_1g16070
malus_domestica MD02G1172200.v1.1 MD15G1284400.v1.1
prunus_persica Prupe.7G133200_v2.0.a1
pyrus_communis pycom02g13880 pycom15g24700 pycom15g24710
rosa_chinensis RchiOBHm_Chr2g0105691
rosa_laevigata RLG00000017449
rosa_rugosa Rorug02G0129200
rosa_samantha Rh2AG180900 Rh2BG190000 Rh2CG185100 Rh2DG186900
rosa_wichuraiana Rw2G014140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 12
AccB1I GGYRCC 1 cut(s) 338
AccB7I CCANNNNNTGG 1 cut(s) 426
AccI GTMKAC 2 cut(s) 6, 570
AccII CGCG 4 cut(s) 470, 624, 721, 1040
AccIII TCCGGA 2 cut(s) 847, 1171
AclWI GGATC 4 cut(s) 590, 609, 807, 1065
AcoI YGGCCR 2 cut(s) 696, 1200
AcuI CTGAAG 1 cut(s) 261
AcyI GRCGYC 2 cut(s) 9, 983
AfaI GTAC 3 cut(s) 129, 905, 1032
AfiI CCNNNNNNNGG 7 cut(s) 37, 426, 454, 544, 768, 769, 892
AflII CTTAAG 1 cut(s) 665
AgsI TTSAA 3 cut(s) 484, 514, 1126
AjnI CCWGG 3 cut(s) 198, 425, 729
AluBI AGCT 5 cut(s) 161, 189, 1095, 1165, 1177
AluI AGCT 5 cut(s) 161, 189, 1095, 1165, 1177
Alw21I GWGCWC 3 cut(s) 842, 1097, 1179
Alw26I GTCTC 7 cut(s) 68, 356, 430, 633, 641, 925, 1173
AlwI GGATC 4 cut(s) 590, 609, 807, 1065
Ama87I CYCGRG 4 cut(s) 37, 72, 234, 1136
Aor13HI TCCGGA 2 cut(s) 847, 1171
AoxI GGCC 9 cut(s) 28, 52, 85, 136, 307, 357, 456, 696, 1200
ApeKI GCWGC 4 cut(s) 380, 791, 1042, 1045
ArsI GACNNNNNNTTYG 4 cut(s) 725, 757, 812, 844
AspLEI GCGC 2 cut(s) 624, 1042
AspS9I GGNCC 5 cut(s) 86, 202, 308, 417, 876
AvaI CYCGRG 4 cut(s) 37, 72, 234, 1136
AvaII GGWCC 3 cut(s) 202, 417, 876
BaeGI GKGCMC 1 cut(s) 343
BanI GGYRCC 1 cut(s) 338
BanII GRGCYC 3 cut(s) 493, 1097, 1179
BarI GAAGNNNNNNTAC 2 cut(s) 39, 71
BauI CACGAG 1 cut(s) 1178
BbsI GAAGAC 1 cut(s) 699
Bbv12I GWGCWC 3 cut(s) 842, 1097, 1179
BbvI GCAGC 4 cut(s) 367, 803, 1029, 1032
BccI CCATC 4 cut(s) 566, 762, 961, 1000
BceAI ACGGC 4 cut(s) 45, 151, 779, 853
BciT130I CCWGG 3 cut(s) 200, 427, 731
BclI TGATCA 1 cut(s) 910
BcoDI GTCTC 7 cut(s) 68, 356, 430, 633, 641, 925, 1173
BfaI CTAG 1 cut(s) 765
BfrI CTTAAG 1 cut(s) 665
BisI GCNGC 9 cut(s) 78, 81, 108, 333, 381, 722, 792, 1043, 1046
BlsI GCNGC 9 cut(s) 79, 82, 109, 334, 382, 723, 793, 1044, 1047
Bme18I GGWCC 3 cut(s) 202, 417, 876
BmeT110I CYCGRG 4 cut(s) 37, 72, 234, 1136
BmgT120I GGNCC 5 cut(s) 86, 202, 308, 417, 876
BmiI GGNNCC 5 cut(s) 310, 340, 554, 728, 754
BpiI GAAGAC 1 cut(s) 699
BplI GAGNNNNNCTC 2 cut(s) 270, 302
BpmI CTGGAG 3 cut(s) 252, 346, 802
BpuEI CTTGAG 1 cut(s) 338
BsaAI YACGTR 1 cut(s) 46
BsaHI GRCGYC 2 cut(s) 9, 983
BsaI GGTCTC 3 cut(s) 356, 430, 641
BsaJI CCNNGG 7 cut(s) 37, 234, 310, 346, 426, 795, 1098
BsaWI WCCGGW 3 cut(s) 191, 847, 1171
BsaXI ACNNNNNCTCC 2 cut(s) 993, 1023
Bsc4I CCNNNNNNNGG 7 cut(s) 37, 426, 454, 544, 768, 769, 892
Bse118I RCCGGY 5 cut(s) 26, 50, 83, 335, 1153
Bse1I ACTGG 3 cut(s) 329, 742, 1203
Bse3DI GCAATG 1 cut(s) 517
BseAI TCCGGA 2 cut(s) 847, 1171
BseBI CCWGG 3 cut(s) 200, 427, 731
BseDI CCNNGG 7 cut(s) 37, 234, 310, 346, 426, 795, 1098
BseLI CCNNNNNNNGG 7 cut(s) 37, 426, 454, 544, 768, 769, 892
BseMI GCAATG 1 cut(s) 517
BseMII CTCAG 3 cut(s) 289, 303, 425
BseNI ACTGG 3 cut(s) 329, 742, 1203
BseRI GAGGAG 1 cut(s) 142
BseSI GKGCMC 1 cut(s) 343
BseX3I CGGCCG 1 cut(s) 696
BseXI GCAGC 4 cut(s) 367, 803, 1029, 1032
Bsh1236I CGCG 4 cut(s) 470, 624, 721, 1040
Bsh1285I CGRYCG 3 cut(s) 16, 378, 699
BshFI GGCC 9 cut(s) 30, 54, 87, 138, 309, 359, 458, 698, 1202
BshNI GGYRCC 1 cut(s) 338
BsiEI CGRYCG 3 cut(s) 16, 378, 699
BsiHKAI GWGCWC 3 cut(s) 842, 1097, 1179
BsiHKCI CYCGRG 4 cut(s) 37, 72, 234, 1136
BsiWI CGTACG 1 cut(s) 127
BslI CCNNNNNNNGG 7 cut(s) 37, 426, 454, 544, 768, 769, 892
BsmAI GTCTC 7 cut(s) 68, 356, 430, 633, 641, 925, 1173
BsmBI CGTCTC 1 cut(s) 68
BsnI GGCC 9 cut(s) 30, 54, 87, 138, 309, 359, 458, 698, 1202
Bso31I GGTCTC 3 cut(s) 356, 430, 641
BsoBI CYCGRG 4 cut(s) 37, 72, 234, 1136
Bsp1286I GDGCHC 5 cut(s) 343, 493, 842, 1097, 1179
Bsp13I TCCGGA 2 cut(s) 847, 1171
Bsp143I GATC 7 cut(s) 13, 375, 582, 601, 799, 910, 1057
Bsp68I TCGCGA 1 cut(s) 470
BspANI GGCC 9 cut(s) 30, 54, 87, 138, 309, 359, 458, 698, 1202
BspCNI CTCAG 3 cut(s) 288, 302, 426
BspEI TCCGGA 2 cut(s) 847, 1171
BspFNI CGCG 4 cut(s) 470, 624, 721, 1040
BspLI GGNNCC 5 cut(s) 310, 340, 554, 728, 754
BspPI GGATC 4 cut(s) 590, 609, 807, 1065
BspQI GCTCTTC 1 cut(s) 847
BspT107I GGYRCC 1 cut(s) 338
BspTI CTTAAG 1 cut(s) 665
BspTNI GGTCTC 3 cut(s) 356, 430, 641
BsrDI GCAATG 1 cut(s) 517
BsrFI RCCGGY 5 cut(s) 26, 50, 83, 335, 1153
BsrI ACTGG 3 cut(s) 329, 742, 1203
BssAI RCCGGY 5 cut(s) 26, 50, 83, 335, 1153
BssECI CCNNGG 7 cut(s) 37, 234, 310, 346, 426, 795, 1098
BssMI GATC 7 cut(s) 13, 375, 582, 601, 799, 910, 1057
BssNI GRCGYC 2 cut(s) 9, 983
BssSI CACGAG 1 cut(s) 1178
Bst2BI CACGAG 1 cut(s) 1178
Bst2UI CCWGG 3 cut(s) 200, 427, 731
Bst4CI ACNGT 1 cut(s) 441
Bst6I CTCTTC 4 cut(s) 126, 479, 829, 847
BstACI GRCGYC 2 cut(s) 9, 983
BstAFI CTTAAG 1 cut(s) 665
BstBAI YACGTR 1 cut(s) 46
BstC8I GCNNGC 3 cut(s) 52, 85, 343
BstDEI CTNAG 4 cut(s) 275, 289, 434, 1158
BstDSI CCRYGG 1 cut(s) 795
BstFNI CGCG 4 cut(s) 470, 624, 721, 1040
BstHHI GCGC 2 cut(s) 624, 1042
BstKTI GATC 7 cut(s) 16, 378, 585, 604, 802, 913, 1060
BstMAI GTCTC 7 cut(s) 68, 356, 430, 633, 641, 925, 1173
BstMBI GATC 7 cut(s) 13, 375, 582, 601, 799, 910, 1057
BstMCI CGRYCG 3 cut(s) 16, 378, 699
BstMWI GCNNNNNNNGC 4 cut(s) 77, 338, 476, 945
BstNI CCWGG 3 cut(s) 200, 427, 731
BstSLI GKGCMC 1 cut(s) 343
BstUI CGCG 4 cut(s) 470, 624, 721, 1040
BstV1I GCAGC 4 cut(s) 367, 803, 1029, 1032
BstV2I GAAGAC 1 cut(s) 699
BstX2I RGATCY 2 cut(s) 582, 601
BstYI RGATCY 2 cut(s) 582, 601
BstZI CGGCCG 1 cut(s) 696
BsuRI GGCC 9 cut(s) 30, 54, 87, 138, 309, 359, 458, 698, 1202
BtgI CCRYGG 1 cut(s) 795
BtsIMutI CAGTG 2 cut(s) 687, 735
BtuMI TCGCGA 1 cut(s) 470
Cac8I GCNNGC 3 cut(s) 52, 85, 343
CfoI GCGC 2 cut(s) 624, 1042
Cfr10I RCCGGY 5 cut(s) 26, 50, 83, 335, 1153
Cfr13I GGNCC 5 cut(s) 86, 202, 308, 417, 876
Cfr9I CCCGGG 2 cut(s) 37, 234
CseI GACGC 3 cut(s) 797, 972, 1046
Csp6I GTAC 3 cut(s) 128, 904, 1031
CviAII CATG 1 cut(s) 935
CviQI GTAC 3 cut(s) 128, 904, 1031
DdeI CTNAG 4 cut(s) 275, 289, 434, 1158
DpnI GATC 7 cut(s) 15, 377, 584, 603, 801, 912, 1059
DpnII GATC 7 cut(s) 13, 375, 582, 601, 799, 910, 1057
EaeI YGGCCR 2 cut(s) 696, 1200
EagI CGGCCG 1 cut(s) 696
Eam1104I CTCTTC 4 cut(s) 126, 479, 829, 847
EarI CTCTTC 4 cut(s) 126, 479, 829, 847
EciI GGCGGA 2 cut(s) 58, 739
Ecl136II GAGCTC 2 cut(s) 1095, 1177
EclXI CGGCCG 1 cut(s) 696
Eco147I AGGCCT 2 cut(s) 359, 458
Eco24I GRGCYC 3 cut(s) 493, 1097, 1179
Eco31I GGTCTC 3 cut(s) 356, 430, 641
Eco47I GGWCC 3 cut(s) 202, 417, 876
Eco52I CGGCCG 1 cut(s) 696
Eco53kI GAGCTC 2 cut(s) 1095, 1177
Eco57I CTGAAG 1 cut(s) 261
Eco88I CYCGRG 4 cut(s) 37, 72, 234, 1136
EcoICRI GAGCTC 2 cut(s) 1095, 1177
EcoRII CCWGG 3 cut(s) 198, 425, 729
EcoT38I GRGCYC 3 cut(s) 493, 1097, 1179
Esp3I CGTCTC 1 cut(s) 68
FaeI CATG 1 cut(s) 938
FaiI YATR 6 cut(s) 173, 660, 866, 936, 959, 1013
FatI CATG 1 cut(s) 934
FauI CCCGC 4 cut(s) 350, 456, 541, 932
FbaI TGATCA 1 cut(s) 910
FblI GTMKAC 2 cut(s) 6, 570
Fnu4HI GCNGC 9 cut(s) 78, 81, 108, 333, 381, 722, 792, 1043, 1046
FriOI GRGCYC 3 cut(s) 493, 1097, 1179
Fsp4HI GCNGC 9 cut(s) 78, 81, 108, 333, 381, 722, 792, 1043, 1046
FspBI CTAG 1 cut(s) 765
GlaI GCGC 2 cut(s) 623, 1041
GluI GCNGC 9 cut(s) 78, 81, 108, 333, 381, 722, 792, 1043, 1046
GsuI CTGGAG 3 cut(s) 252, 346, 802
HaeIII GGCC 9 cut(s) 30, 54, 87, 138, 309, 359, 458, 698, 1202
HgaI GACGC 3 cut(s) 797, 972, 1046
HhaI GCGC 2 cut(s) 624, 1042
Hin1I GRCGYC 2 cut(s) 9, 983
Hin1II CATG 1 cut(s) 938
Hin6I GCGC 2 cut(s) 622, 1040
HinP1I GCGC 2 cut(s) 622, 1040
HincII GTYRAC 2 cut(s) 7, 571
HindII GTYRAC 2 cut(s) 7, 571
HinfI GANTC 5 cut(s) 221, 577, 626, 1070, 1205
Hpy166II GTNNAC 3 cut(s) 7, 571, 898
Hpy188III TCNNGA 9 cut(s) 269, 320, 373, 469, 750, 781, 848, 886, 1172
Hpy8I GTNNAC 3 cut(s) 7, 571, 898
HpyAV CCTTC 4 cut(s) 65, 149, 157, 1135
HpyCH4III ACNGT 1 cut(s) 441
HpyCH4IV ACGT 4 cut(s) 9, 45, 168, 567
HpyCH4V TGCA 1 cut(s) 534
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 338, 476, 945
HpyF3I CTNAG 4 cut(s) 275, 289, 434, 1158
HpySE526I ACGT 4 cut(s) 9, 45, 168, 567
Hsp92I GRCGYC 2 cut(s) 9, 983
Hsp92II CATG 1 cut(s) 938
HspAI GCGC 2 cut(s) 622, 1040
Kpn2I TCCGGA 2 cut(s) 847, 1171
KroI GCCGGC 2 cut(s) 50, 83
KroNI GCCGGC 2 cut(s) 52, 85
Ksp22I TGATCA 1 cut(s) 910
Kzo9I GATC 7 cut(s) 13, 375, 582, 601, 799, 910, 1057
LguI GCTCTTC 1 cut(s) 847
LmnI GCTCC 8 cut(s) 194, 726, 783, 945, 1080, 1092, 1100, 1174
Lsp1109I GCAGC 4 cut(s) 367, 803, 1029, 1032
MaeI CTAG 1 cut(s) 765
MaeII ACGT 4 cut(s) 9, 45, 168, 567
MaeIII GTNAC 1 cut(s) 41
MalI GATC 7 cut(s) 15, 377, 584, 603, 801, 912, 1059
MboI GATC 7 cut(s) 13, 375, 582, 601, 799, 910, 1057
MboII GAAGA 9 cut(s) 113, 496, 553, 577, 607, 699, 816, 834, 850
MflI RGATCY 2 cut(s) 582, 601
MhlI GDGCHC 5 cut(s) 343, 493, 842, 1097, 1179
MluCI AATT 3 cut(s) 507, 712, 1126
MlyI GAGTC 3 cut(s) 635, 1064, 1214
MmeI TCCRAC 2 cut(s) 468, 996
MroI TCCGGA 2 cut(s) 847, 1171
MroNI GCCGGC 2 cut(s) 50, 83
MseI TTAA 1 cut(s) 666
MspCI CTTAAG 1 cut(s) 665
MvaI CCWGG 3 cut(s) 200, 427, 731
MvnI CGCG 4 cut(s) 470, 624, 721, 1040
MwoI GCNNNNNNNGC 4 cut(s) 77, 338, 476, 945
NaeI GCCGGC 2 cut(s) 52, 85
NdeII GATC 7 cut(s) 13, 375, 582, 601, 799, 910, 1057
NgoMIV GCCGGC 2 cut(s) 50, 83
NlaIII CATG 1 cut(s) 938
NlaIV GGNNCC 5 cut(s) 310, 340, 554, 728, 754
NmuCI GTSAC 1 cut(s) 41
NruI TCGCGA 1 cut(s) 470
PceI AGGCCT 2 cut(s) 359, 458
PciSI GCTCTTC 1 cut(s) 847
PcsI WCGNNNNNNNCGW 3 cut(s) 701, 833, 980
PdiI GCCGGC 2 cut(s) 52, 85
PfeI GAWTC 2 cut(s) 221, 577
Pfl23II CGTACG 1 cut(s) 127
PflFI GACNNNGTC 1 cut(s) 167
PflMI CCANNNNNTGG 1 cut(s) 426
PkrI GCNGC 9 cut(s) 79, 82, 109, 334, 382, 723, 793, 1044, 1047
Ple19I CGATCG 2 cut(s) 16, 378
PleI GAGTC 3 cut(s) 634, 1064, 1213
PpsI GAGTC 3 cut(s) 634, 1064, 1213
Ppu21I YACGTR 1 cut(s) 46
Psp124BI GAGCTC 2 cut(s) 1097, 1179
Psp6I CCWGG 3 cut(s) 198, 425, 729
PspGI CCWGG 3 cut(s) 198, 425, 729
PspLI CGTACG 1 cut(s) 127
PspN4I GGNNCC 5 cut(s) 310, 340, 554, 728, 754
PspPI GGNCC 5 cut(s) 86, 202, 308, 417, 876
PsuI RGATCY 2 cut(s) 582, 601
PsyI GACNNNGTC 1 cut(s) 167
PvuI CGATCG 2 cut(s) 16, 378
RruI TCGCGA 1 cut(s) 470
RsaI GTAC 3 cut(s) 129, 905, 1032
RsaNI GTAC 3 cut(s) 128, 904, 1031
SacI GAGCTC 2 cut(s) 1097, 1179
SalI GTCGAC 2 cut(s) 5, 569
SapI GCTCTTC 1 cut(s) 847
SaqAI TTAA 1 cut(s) 666
SatI GCNGC 9 cut(s) 78, 81, 108, 333, 381, 722, 792, 1043, 1046
Sau3AI GATC 7 cut(s) 13, 375, 582, 601, 799, 910, 1057
Sau96I GGNCC 5 cut(s) 86, 202, 308, 417, 876
SchI GAGTC 3 cut(s) 635, 1064, 1214
SduI GDGCHC 5 cut(s) 343, 493, 842, 1097, 1179
SgrAI CRCCGGYG 1 cut(s) 335
SgrDI CGTCGACG 1 cut(s) 569
SinI GGWCC 3 cut(s) 202, 417, 876
SmaI CCCGGG 2 cut(s) 39, 236
SmlI CTYRAG 2 cut(s) 353, 665
SmoI CTYRAG 2 cut(s) 353, 665
SrfI GCCCGGGC 1 cut(s) 236
Sse9I AATT 3 cut(s) 507, 712, 1126
SseBI AGGCCT 2 cut(s) 359, 458
SspMI CTAG 1 cut(s) 765
SstI GAGCTC 2 cut(s) 1097, 1179
StuI AGGCCT 2 cut(s) 359, 458
TaaI ACNGT 1 cut(s) 441
TaiI ACGT 4 cut(s) 12, 48, 171, 570
TaqII GACCGA 2 cut(s) 338, 665
TasI AATT 3 cut(s) 507, 712, 1126
TatI WGTACW 1 cut(s) 903
TauI GCSGC 5 cut(s) 80, 83, 110, 335, 724
TfiI GAWTC 2 cut(s) 221, 577
Tru1I TTAA 1 cut(s) 666
Tru9I TTAA 1 cut(s) 666
TscAI CASTG 2 cut(s) 687, 742
TseFI GTSAC 1 cut(s) 41
TseI GCWGC 4 cut(s) 380, 791, 1042, 1045
Tsp45I GTSAC 1 cut(s) 41
TspGWI ACGGA 3 cut(s) 145, 519, 968
TspMI CCCGGG 2 cut(s) 37, 234
TspRI CASTG 2 cut(s) 687, 742
Tth111I GACNNNGTC 1 cut(s) 167
Van91I CCANNNNNTGG 1 cut(s) 426
Vha464I CTTAAG 1 cut(s) 665
VpaK11BI GGWCC 3 cut(s) 202, 417, 876
XmaI CCCGGG 2 cut(s) 37, 234
XmiI GTMKAC 2 cut(s) 6, 570
XspI CTAG 1 cut(s) 765
ZraI GACGTC 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.