AT3G50860

Belongs to the adaptor complexes small subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009137
Physical Location & Seq
Forward (+)
18901997 .. 18904197
2201 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G50860.1

Sequence Viewer

Length: 501 bp
ATGATTAAGGCAGTGATGATGATGAACACACAAGGCAAACCACGTCTAGCTAAATTCTACGATTACTTGCCTGTGGAGAAGCAGCAGGAGCTTATTCGCGGCGTGTTTTCAGTATTGTGCAGTAGACCTGAGAACGTAAGCAATTTTCTGGAGATCGAATCATTGTTTGGACCGGACTCGCGGCTTGTATACAAGCATTATGCTACACTCTATTTTGTTCTTGTATTTGATGGTTCAGAAAATGAGCTTGCTATGCTTGATCTCATTCAAGTTCTTGTTGAAACACTGGACAAATGCTTCAGCAATGTCTGCGAACTCGACATTGTGTTCAACTACAGCAAGATGCACGCGGTGTTAGATGAGATTGTATTTGGAGGACAAGTACTGGAAACTAGTTCTGCTGAAGTCATGAAGGCTGTTGAAGAAATATCAAAATTAGAAGCTGCCTCAAATTCGATTTCACTTGTCCCCAAGTCTGTTTCCGGGTGGCGTGGCCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
Clat_adaptor_s

Protein Analysis

166

Amino Acids

18.73

Weight (kDa)

4.98

Isoelectric Point (pI)

40.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clat_adaptor_s PF01217 1 - 146 5.1e-48 Clathrin adaptor complex small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 124, 189
AccII CGCG 3 cut(s) 99, 181, 350
AciI CCGC 3 cut(s) 99, 181, 350
AcoI YGGCCR 1 cut(s) 493
AcsI RAATTY 2 cut(s) 53, 451
AcuI CTGAAG 2 cut(s) 283, 423
AdeI CACNNNGTG 1 cut(s) 352
AfaI GTAC 1 cut(s) 384
AgsI TTSAA 4 cut(s) 269, 281, 331, 422
AhlI ACTAGT 1 cut(s) 392
AjiI CACGTC 1 cut(s) 44
AjuI GAANNNNNNNTTGG 2 cut(s) 150, 182
AluBI AGCT 4 cut(s) 50, 91, 247, 443
AluI AGCT 4 cut(s) 50, 91, 247, 443
AoxI GGCC 1 cut(s) 493
ApeKI GCWGC 2 cut(s) 82, 443
ApoI RAATTY 2 cut(s) 53, 451
AspS9I GGNCC 1 cut(s) 170
AsuC2I CCSGG 1 cut(s) 484
AvaII GGWCC 1 cut(s) 170
BbvI GCAGC 2 cut(s) 94, 430
BccI CCATC 1 cut(s) 224
BceAI ACGGC 1 cut(s) 480
BcnI CCSGG 1 cut(s) 484
BcuI ACTAGT 1 cut(s) 392
BfaI CTAG 2 cut(s) 47, 393
BfmI CTRYAG 1 cut(s) 334
BisI GCNGC 4 cut(s) 83, 100, 182, 444
BlsI GCNGC 4 cut(s) 84, 101, 183, 445
BmcAI AGTACT 1 cut(s) 384
Bme1390I CCNGG 1 cut(s) 484
Bme18I GGWCC 1 cut(s) 170
BmgBI CACGTC 1 cut(s) 44
BmgT120I GGNCC 1 cut(s) 170
BmrFI CCNGG 1 cut(s) 484
BmsI GCATC 1 cut(s) 333
BpmI CTGGAG 1 cut(s) 170
BpuMI CCSGG 1 cut(s) 484
BsaWI WCCGGW 1 cut(s) 172
BsaXI ACNNNNNCTCC 2 cut(s) 366, 396
Bse1I ACTGG 2 cut(s) 291, 390
Bse3DI GCAATG 1 cut(s) 310
BseMI GCAATG 1 cut(s) 310
BseMII CTCAG 1 cut(s) 120
BseNI ACTGG 2 cut(s) 291, 390
BseXI GCAGC 2 cut(s) 94, 430
BsgI GTGCAG 1 cut(s) 139
Bsh1236I CGCG 3 cut(s) 99, 181, 350
BshFI GGCC 1 cut(s) 495
BsiSI CCGG 2 cut(s) 173, 483
BslFI GGGAC 1 cut(s) 452
BsmFI GGGAC 1 cut(s) 452
BsnI GGCC 1 cut(s) 495
Bsp143I GATC 2 cut(s) 153, 259
BspACI CCGC 3 cut(s) 99, 181, 350
BspANI GGCC 1 cut(s) 495
BspCNI CTCAG 1 cut(s) 121
BspFNI CGCG 3 cut(s) 99, 181, 350
BspHI TCATGA 1 cut(s) 408
BsrDI GCAATG 1 cut(s) 310
BsrI ACTGG 2 cut(s) 291, 390
BssMI GATC 2 cut(s) 153, 259
BssNAI GTATAC 1 cut(s) 190
Bst1107I GTATAC 1 cut(s) 190
BstAPI GCANNNNNTGC 1 cut(s) 309
BstC8I GCNNGC 2 cut(s) 249, 348
BstDEI CTNAG 1 cut(s) 129
BstFNI CGCG 3 cut(s) 99, 181, 350
BstKTI GATC 2 cut(s) 156, 262
BstMBI GATC 2 cut(s) 153, 259
BstMWI GCNNNNNNNGC 3 cut(s) 88, 253, 309
BstSCI CCNGG 1 cut(s) 482
BstSFI CTRYAG 1 cut(s) 334
BstUI CGCG 3 cut(s) 99, 181, 350
BstV1I GCAGC 2 cut(s) 94, 430
BstZ17I GTATAC 1 cut(s) 190
BsuRI GGCC 1 cut(s) 495
BtrI CACGTC 1 cut(s) 44
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 2 cut(s) 18, 284
Cac8I GCNNGC 2 cut(s) 249, 348
CciI TCATGA 1 cut(s) 408
Cfr13I GGNCC 1 cut(s) 170
Csp6I GTAC 1 cut(s) 383
CviAII CATG 1 cut(s) 409
CviJI RGCY 7 cut(s) 50, 91, 184, 247, 416, 443, 495
CviKI_1 RGCY 7 cut(s) 50, 91, 184, 247, 416, 443, 495
CviQI GTAC 1 cut(s) 383
DdeI CTNAG 1 cut(s) 129
DpnI GATC 2 cut(s) 155, 261
DpnII GATC 2 cut(s) 153, 259
DraIII CACNNNGTG 1 cut(s) 352
EaeI YGGCCR 1 cut(s) 493
Eco47I GGWCC 1 cut(s) 170
Eco57I CTGAAG 2 cut(s) 283, 423
FaeI CATG 1 cut(s) 412
FaiI YATR 4 cut(s) 190, 201, 254, 410
FaqI GGGAC 1 cut(s) 452
FatI CATG 1 cut(s) 408
FblI GTMKAC 2 cut(s) 124, 189
Fnu4HI GCNGC 4 cut(s) 83, 100, 182, 444
Fsp4HI GCNGC 4 cut(s) 83, 100, 182, 444
FspBI CTAG 2 cut(s) 47, 393
GluI GCNGC 4 cut(s) 83, 100, 182, 444
GsuI CTGGAG 1 cut(s) 170
HaeIII GGCC 1 cut(s) 495
HapII CCGG 2 cut(s) 173, 483
Hin1II CATG 1 cut(s) 412
HinfI GANTC 2 cut(s) 158, 176
HpaII CCGG 2 cut(s) 173, 483
Hpy166II GTNNAC 2 cut(s) 125, 190
Hpy188I TCNGA 1 cut(s) 238
Hpy188III TCNNGA 2 cut(s) 149, 409
Hpy8I GTNNAC 2 cut(s) 125, 190
HpyAV CCTTC 1 cut(s) 406
HpyCH4IV ACGT 2 cut(s) 43, 135
HpyCH4V TGCA 2 cut(s) 120, 346
HpyF10VI GCNNNNNNNGC 3 cut(s) 88, 253, 309
HpyF3I CTNAG 1 cut(s) 129
HpySE526I ACGT 2 cut(s) 43, 135
Hsp92II CATG 1 cut(s) 412
Kzo9I GATC 2 cut(s) 153, 259
LmnI GCTCC 1 cut(s) 88
LpnPI CCDG 8 cut(s) 71, 84, 134, 141, 186, 272, 371, 496
Lsp1109I GCAGC 2 cut(s) 94, 430
LweI GCATC 1 cut(s) 333
MaeI CTAG 2 cut(s) 47, 393
MaeII ACGT 2 cut(s) 43, 135
MalI GATC 2 cut(s) 155, 261
MboI GATC 2 cut(s) 153, 259
MboII GAAGA 1 cut(s) 434
MluCI AATT 4 cut(s) 53, 142, 434, 451
MlyI GAGTC 1 cut(s) 170
MnlI CCTC 2 cut(s) 368, 457
MseI TTAA 1 cut(s) 6
MspI CCGG 2 cut(s) 173, 483
MspR9I CCNGG 1 cut(s) 484
MvnI CGCG 3 cut(s) 99, 181, 350
MwoI GCNNNNNNNGC 3 cut(s) 88, 253, 309
NciI CCSGG 1 cut(s) 484
NdeII GATC 2 cut(s) 153, 259
NlaIII CATG 1 cut(s) 412
PagI TCATGA 1 cut(s) 408
PfeI GAWTC 1 cut(s) 158
PkrI GCNGC 4 cut(s) 84, 101, 183, 445
PleI GAGTC 1 cut(s) 170
PpsI GAGTC 1 cut(s) 170
PspPI GGNCC 1 cut(s) 170
RsaI GTAC 1 cut(s) 384
RsaNI GTAC 1 cut(s) 383
SaqAI TTAA 1 cut(s) 6
SatI GCNGC 4 cut(s) 83, 100, 182, 444
Sau3AI GATC 2 cut(s) 153, 259
Sau96I GGNCC 1 cut(s) 170
ScaI AGTACT 1 cut(s) 384
SchI GAGTC 1 cut(s) 170
ScrFI CCNGG 1 cut(s) 484
SetI ASST 7 cut(s) 46, 52, 93, 130, 138, 249, 445
SfaNI GCATC 1 cut(s) 333
SfcI CTRYAG 1 cut(s) 334
SinI GGWCC 1 cut(s) 170
SpeI ACTAGT 1 cut(s) 392
Sse9I AATT 4 cut(s) 53, 142, 434, 451
SsiI CCGC 3 cut(s) 99, 181, 350
SspMI CTAG 2 cut(s) 47, 393
StyD4I CCNGG 1 cut(s) 482
TaiI ACGT 2 cut(s) 46, 138
TaqI TCGA 3 cut(s) 156, 318, 455
TasI AATT 4 cut(s) 53, 142, 434, 451
TatI WGTACW 1 cut(s) 382
TauI GCSGC 2 cut(s) 102, 184
TfiI GAWTC 1 cut(s) 158
Tru1I TTAA 1 cut(s) 6
Tru9I TTAA 1 cut(s) 6
TscAI CASTG 2 cut(s) 18, 291
TseI GCWGC 2 cut(s) 82, 443
TspDTI ATGAA 2 cut(s) 38, 425
TspRI CASTG 2 cut(s) 18, 291
VpaK11BI GGWCC 1 cut(s) 170
XapI RAATTY 2 cut(s) 53, 451
XmiI GTMKAC 2 cut(s) 124, 189
XspI CTAG 2 cut(s) 47, 393
ZrmI AGTACT 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.