MD15G1261700.v1.1

Belongs to the adaptor complexes small subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009137
Physical Location & Seq
Reverse (-)
22373328 .. 22375416
2089 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1261700.v1.1.491

Sequence Viewer

Length: 594 bp
ATGGATCTCAAATCAAATCTCATTTTTTGTAGTGTGGAAATTCACTTAAAAAAAAAGAAAGAAACGTTTTTTTGCTTATTATTTCTGGAAAAGATGATACGATCGGTGATGGTGATAAACACGCAGGGGAAGCCTCGCCTTGCCAAGTTCTACGAATTTCAGCCTGTGGAGAAGCAACAGGAGCTTATACGCAGCGTCTATGGAGTGTTATCCAGTAGAGCTGAGAATGTGAGCAATTTCGTGGACGCCGAGTCTATTTTCGGCGCGGATACTCGCCTTGTGTACAAGCACTTTGCAACTTTGTACTTCGTCTTTGTATTCGATAGTTCTGAAAACGAGCTTGCCATGCTTGACCTCATTCAAGTTTTTGTGGAAACATTGGACAAATGCTTCAAAAATGTATGCGAGCTTGATGTGGTCCTCAATTACAGCAAGATGCATACCGTATTGGATGAGATCATTTTTGGAGGTCAAGTGCTGGAAACAAGTTCTACTGAAGTTATGAAGGCCGTTGAAGAAATCTCAAGGTTAGAATCTGCCTCAAATGCCATCTCACTGGTCCCAAAATCTGTTTCCGCCTGGCGGAGTCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
Clat_adaptor_s

Protein Analysis

198

Amino Acids

22.58

Weight (kDa)

5.71

Isoelectric Point (pI)

38.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clat_adaptor_s PF01217 32 - 176 1.4e-46 Clathrin adaptor complex small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 266
AciI CCGC 3 cut(s) 266, 576, 583
AclI AACGTT 1 cut(s) 65
AclWI GGATC 1 cut(s) 12
AcsI RAATTY 2 cut(s) 39, 155
AcuI CTGAAG 1 cut(s) 516
AcyI GRCGYC 1 cut(s) 246
AfaI GTAC 2 cut(s) 284, 305
AfiI CCNNNNNNNGG 1 cut(s) 582
AgsI TTSAA 3 cut(s) 362, 394, 515
AhdI GACNNNNNGTC 1 cut(s) 250
AjnI CCWGG 1 cut(s) 578
AjuI GAANNNNNNNTTGG 2 cut(s) 556, 588
AluBI AGCT 4 cut(s) 184, 221, 340, 409
AluI AGCT 4 cut(s) 184, 221, 340, 409
AlwI GGATC 1 cut(s) 12
AoxI GGCC 1 cut(s) 507
ApeKI GCWGC 1 cut(s) 192
ApoI RAATTY 2 cut(s) 39, 155
AspLEI GCGC 1 cut(s) 266
AspS9I GGNCC 2 cut(s) 418, 559
AsuHPI GGTGA 2 cut(s) 118, 124
AvaII GGWCC 2 cut(s) 418, 559
BbvI GCAGC 1 cut(s) 204
BccI CCATC 2 cut(s) 103, 557
BceAI ACGGC 1 cut(s) 494
BciT130I CCWGG 1 cut(s) 580
BciVI GTATCC 1 cut(s) 262
BfuI GTATCC 1 cut(s) 262
BisI GCNGC 1 cut(s) 193
BlsI GCNGC 1 cut(s) 194
Bme1390I CCNGG 1 cut(s) 580
Bme18I GGWCC 2 cut(s) 418, 559
BmeRI GACNNNNNGTC 1 cut(s) 250
BmgT120I GGNCC 2 cut(s) 418, 559
BmiI GGNNCC 1 cut(s) 561
BmrFI CCNGG 1 cut(s) 580
BmsI GCATC 1 cut(s) 426
BpuEI CTTGAG 1 cut(s) 508
BsaHI GRCGYC 1 cut(s) 246
BsaXI ACNNNNNCTCC 2 cut(s) 459, 489
Bsc4I CCNNNNNNNGG 1 cut(s) 582
Bse1I ACTGG 2 cut(s) 213, 561
BseBI CCWGG 1 cut(s) 580
BseGI GGATG 1 cut(s) 457
BseLI CCNNNNNNNGG 1 cut(s) 582
BseMII CTCAG 1 cut(s) 213
BseNI ACTGG 2 cut(s) 213, 561
BseXI GCAGC 1 cut(s) 204
Bsh1236I CGCG 1 cut(s) 266
Bsh1285I CGRYCG 1 cut(s) 104
BshFI GGCC 1 cut(s) 509
BsiEI CGRYCG 1 cut(s) 104
BslFI GGGAC 1 cut(s) 545
BslI CCNNNNNNNGG 1 cut(s) 582
BsmFI GGGAC 1 cut(s) 545
BsnI GGCC 1 cut(s) 509
Bsp1407I TGTACA 1 cut(s) 282
Bsp143I GATC 3 cut(s) 4, 101, 456
BspACI CCGC 3 cut(s) 266, 576, 583
BspANI GGCC 1 cut(s) 509
BspCNI CTCAG 1 cut(s) 214
BspFNI CGCG 1 cut(s) 266
BspLI GGNNCC 1 cut(s) 561
BspPI GGATC 1 cut(s) 12
BsrGI TGTACA 1 cut(s) 282
BsrI ACTGG 2 cut(s) 213, 561
BssMI GATC 3 cut(s) 4, 101, 456
BssNI GRCGYC 1 cut(s) 246
Bst2UI CCWGG 1 cut(s) 580
Bst4CI ACNGT 1 cut(s) 445
BstACI GRCGYC 1 cut(s) 246
BstAUI TGTACA 1 cut(s) 282
BstC8I GCNNGC 2 cut(s) 342, 407
BstDEI CTNAG 1 cut(s) 222
BstF5I GGATG 1 cut(s) 457
BstFNI CGCG 1 cut(s) 266
BstHHI GCGC 1 cut(s) 266
BstKTI GATC 3 cut(s) 7, 104, 459
BstMBI GATC 3 cut(s) 4, 101, 456
BstMCI CGRYCG 1 cut(s) 104
BstMWI GCNNNNNNNGC 4 cut(s) 130, 181, 346, 545
BstNI CCWGG 1 cut(s) 580
BstSCI CCNGG 1 cut(s) 578
BstUI CGCG 1 cut(s) 266
BstV1I GCAGC 1 cut(s) 204
BstX2I RGATCY 1 cut(s) 4
BstXI CCANNNNNNTGG 1 cut(s) 556
BstYI RGATCY 1 cut(s) 4
BsuI GTATCC 1 cut(s) 262
BsuRI GGCC 1 cut(s) 509
BtsCI GGATG 1 cut(s) 457
BtsIMutI CAGTG 1 cut(s) 554
Cac8I GCNNGC 2 cut(s) 342, 407
CfoI GCGC 1 cut(s) 266
Cfr13I GGNCC 2 cut(s) 418, 559
CseI GACGC 2 cut(s) 184, 254
Csp6I GTAC 2 cut(s) 283, 304
CviAII CATG 1 cut(s) 346
CviJI RGCY 7 cut(s) 133, 163, 184, 221, 340, 409, 509
CviKI_1 RGCY 7 cut(s) 133, 163, 184, 221, 340, 409, 509
CviQI GTAC 2 cut(s) 283, 304
DdeI CTNAG 1 cut(s) 222
DpnI GATC 3 cut(s) 6, 103, 458
DpnII GATC 3 cut(s) 4, 101, 456
DriI GACNNNNNGTC 1 cut(s) 250
Eam1105I GACNNNNNGTC 1 cut(s) 250
EciI GGCGGA 1 cut(s) 565
Eco47I GGWCC 2 cut(s) 418, 559
Eco57I CTGAAG 1 cut(s) 516
EcoRII CCWGG 1 cut(s) 578
EcoT22I ATGCAT 1 cut(s) 441
FaeI CATG 1 cut(s) 349
FaiI YATR 6 cut(s) 188, 201, 347, 403, 441, 503
FaqI GGGAC 1 cut(s) 545
FatI CATG 1 cut(s) 345
Fnu4HI GCNGC 1 cut(s) 193
FokI GGATG 1 cut(s) 464
Fsp4HI GCNGC 1 cut(s) 193
GlaI GCGC 1 cut(s) 265
GluI GCNGC 1 cut(s) 193
HaeIII GGCC 1 cut(s) 509
HgaI GACGC 2 cut(s) 184, 254
HhaI GCGC 1 cut(s) 266
Hin1I GRCGYC 1 cut(s) 246
Hin1II CATG 1 cut(s) 349
Hin6I GCGC 1 cut(s) 264
HinP1I GCGC 1 cut(s) 264
HinfI GANTC 3 cut(s) 251, 533, 586
HphI GGTGA 2 cut(s) 118, 124
Hpy166II GTNNAC 2 cut(s) 244, 283
Hpy188I TCNGA 1 cut(s) 331
Hpy188III TCNNGA 1 cut(s) 86
Hpy8I GTNNAC 2 cut(s) 244, 283
HpyAV CCTTC 1 cut(s) 499
HpyCH4III ACNGT 1 cut(s) 445
HpyCH4IV ACGT 1 cut(s) 65
HpyCH4V TGCA 2 cut(s) 296, 439
HpyF10VI GCNNNNNNNGC 4 cut(s) 130, 181, 346, 545
HpyF3I CTNAG 1 cut(s) 222
HpySE526I ACGT 1 cut(s) 65
Hsp92I GRCGYC 1 cut(s) 246
Hsp92II CATG 1 cut(s) 349
HspAI GCGC 1 cut(s) 264
Kzo9I GATC 3 cut(s) 4, 101, 456
LmnI GCTCC 1 cut(s) 181
LpnPI CCDG 8 cut(s) 71, 110, 164, 177, 226, 464, 542, 565
Lsp1109I GCAGC 1 cut(s) 204
LweI GCATC 1 cut(s) 426
MaeII ACGT 1 cut(s) 65
MalI GATC 3 cut(s) 6, 103, 458
MboI GATC 3 cut(s) 4, 101, 456
MboII GAAGA 1 cut(s) 527
MflI RGATCY 1 cut(s) 4
MluCI AATT 4 cut(s) 39, 155, 235, 424
MlyI GAGTC 1 cut(s) 260
MnlI CCTC 5 cut(s) 144, 365, 431, 461, 550
Mph1103I ATGCAT 1 cut(s) 441
MseI TTAA 1 cut(s) 47
MspR9I CCNGG 1 cut(s) 580
MvaI CCWGG 1 cut(s) 580
MvnI CGCG 1 cut(s) 266
MwoI GCNNNNNNNGC 4 cut(s) 130, 181, 346, 545
NdeII GATC 3 cut(s) 4, 101, 456
NlaIII CATG 1 cut(s) 349
NlaIV GGNNCC 1 cut(s) 561
NmeAIII GCCGAG 1 cut(s) 274
NsiI ATGCAT 1 cut(s) 441
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PfeI GAWTC 1 cut(s) 533
PkrI GCNGC 1 cut(s) 194
Ple19I CGATCG 1 cut(s) 104
PleI GAGTC 1 cut(s) 259
PpsI GAGTC 1 cut(s) 259
Psp1406I AACGTT 1 cut(s) 65
Psp6I CCWGG 1 cut(s) 578
PspGI CCWGG 1 cut(s) 578
PspN4I GGNNCC 1 cut(s) 561
PspPI GGNCC 2 cut(s) 418, 559
PsuI RGATCY 1 cut(s) 4
PvuI CGATCG 1 cut(s) 104
RsaI GTAC 2 cut(s) 284, 305
RsaNI GTAC 2 cut(s) 283, 304
SaqAI TTAA 1 cut(s) 47
SatI GCNGC 1 cut(s) 193
Sau3AI GATC 3 cut(s) 4, 101, 456
Sau96I GGNCC 2 cut(s) 418, 559
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 1 cut(s) 580
SetI ASST 8 cut(s) 68, 186, 223, 342, 357, 411, 472, 530
SfaNI GCATC 1 cut(s) 426
SinI GGWCC 2 cut(s) 418, 559
SmlI CTYRAG 1 cut(s) 523
SmoI CTYRAG 1 cut(s) 523
Sse9I AATT 4 cut(s) 39, 155, 235, 424
SsiI CCGC 3 cut(s) 266, 576, 583
StyD4I CCNGG 1 cut(s) 578
TaaI ACNGT 1 cut(s) 445
TaiI ACGT 1 cut(s) 68
TaqI TCGA 2 cut(s) 321, 589
TasI AATT 4 cut(s) 39, 155, 235, 424
TatI WGTACW 2 cut(s) 282, 303
TfiI GAWTC 1 cut(s) 533
Tru1I TTAA 1 cut(s) 47
Tru9I TTAA 1 cut(s) 47
TscAI CASTG 1 cut(s) 561
TseI GCWGC 1 cut(s) 192
TspDTI ATGAA 1 cut(s) 518
TspRI CASTG 1 cut(s) 561
VpaK11BI GGWCC 2 cut(s) 418, 559
XapI RAATTY 2 cut(s) 39, 155
Zsp2I ATGCAT 1 cut(s) 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.