MD02G1147400.v1.1

Belongs to the adaptor complexes small subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009137
Physical Location & Seq
Reverse (-)
12168322 .. 12170863
2542 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1147400.v1.1.491

Sequence Viewer

Length: 606 bp
ATGTACATAATTACAAGGGACCAGGCCGGCGTGGATCTTAAGCCAGTTCTCATCTGTGCAGTCTGCAAATTCACTGAAAAAAAGATTTTCTTGTTTCTGGAGAAGATGATCCGATCGGTGATGGTGATAAACACGCAGGGGAAGCCTCGCCTTTCCAAGTTCTACGAATTTCAGCCTGTGGAGAAGCAACAGGAACTTATAAGCAGCGTCCATGGAGTGTTGTCCGGTAGAGCTGAGAATGTGAGCAATTTCGTGGATGCTGAGTCTATTTTCGGCCCGGATACTCGCCTTGTGTACAAGCACTTTGCAACTTTGTACATCGTCTTTGTGTTCGATAGCTCTGAAAACGAGCTTGCCATGCTCGACCTCATACAAGTATTTGTGGAAACATTGGACAAATGCTTCAAAAATGTATGCGAGCTTGACGTGGTTATCAATTACAGCAAGATGCATACCGTATTGGATGAGATCATTTTTGGAGGTCAAGTGCTGGAAACAAGTTCAACTGAAGTTATGAAGGCCGTCGAAGAAATCTCAAGGTTAGAAGCAGCCTCAAATGCCATCTCACTGGTCCCAAAATCTGTTTCCGCCTGGCGGAGTCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
Clat_adaptor_s

Protein Analysis

202

Amino Acids

22.77

Weight (kDa)

5.49

Isoelectric Point (pI)

33.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clat_adaptor_s PF01217 36 - 180 2.3e-44 Clathrin adaptor complex small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 200
AciI CCGC 2 cut(s) 588, 595
AclWI GGATC 2 cut(s) 42, 103
AcsI RAATTY 2 cut(s) 68, 167
AcuI CTGAAG 1 cut(s) 528
AfaI GTAC 3 cut(s) 5, 296, 317
AfiI CCNNNNNNNGG 1 cut(s) 594
AflII CTTAAG 1 cut(s) 38
AgsI TTSAA 2 cut(s) 406, 504
AjiI CACGTC 1 cut(s) 427
AjnI CCWGG 2 cut(s) 21, 590
AjuI GAANNNNNNNTTGG 2 cut(s) 568, 600
AluBI AGCT 4 cut(s) 233, 339, 352, 421
AluI AGCT 4 cut(s) 233, 339, 352, 421
AlwI GGATC 2 cut(s) 42, 103
AoxI GGCC 3 cut(s) 24, 274, 519
ApeKI GCWGC 2 cut(s) 204, 548
ApoI RAATTY 2 cut(s) 68, 167
AspS9I GGNCC 3 cut(s) 19, 275, 571
AsuC2I CCSGG 1 cut(s) 278
AsuHPI GGTGA 2 cut(s) 130, 136
AvaII GGWCC 2 cut(s) 19, 571
BbvI GCAGC 2 cut(s) 216, 560
BccI CCATC 2 cut(s) 115, 569
BceAI ACGGC 1 cut(s) 506
BciT130I CCWGG 2 cut(s) 23, 592
BciVI GTATCC 1 cut(s) 274
BcnI CCSGG 1 cut(s) 278
BfrI CTTAAG 1 cut(s) 38
BfuI GTATCC 1 cut(s) 274
BisI GCNGC 2 cut(s) 205, 549
BlsI GCNGC 2 cut(s) 206, 550
Bme1390I CCNGG 3 cut(s) 23, 278, 592
Bme18I GGWCC 2 cut(s) 19, 571
BmgBI CACGTC 1 cut(s) 427
BmgT120I GGNCC 3 cut(s) 19, 275, 571
BmiI GGNNCC 2 cut(s) 20, 573
BmrFI CCNGG 3 cut(s) 23, 278, 592
BmsI GCATC 2 cut(s) 247, 438
BpmI CTGGAG 1 cut(s) 119
BpuEI CTTGAG 1 cut(s) 520
BpuMI CCSGG 1 cut(s) 278
BsaJI CCNNGG 1 cut(s) 211
BsaWI WCCGGW 1 cut(s) 224
BsaXI ACNNNNNCTCC 2 cut(s) 471, 501
Bsc4I CCNNNNNNNGG 1 cut(s) 594
Bse118I RCCGGY 1 cut(s) 26
Bse1I ACTGG 2 cut(s) 44, 573
BseBI CCWGG 2 cut(s) 23, 592
BseDI CCNNGG 1 cut(s) 211
BseGI GGATG 2 cut(s) 262, 469
BseLI CCNNNNNNNGG 1 cut(s) 594
BseMII CTCAG 2 cut(s) 225, 252
BseNI ACTGG 2 cut(s) 44, 573
BseXI GCAGC 2 cut(s) 216, 560
BsgI GTGCAG 1 cut(s) 78
Bsh1285I CGRYCG 1 cut(s) 116
BshFI GGCC 3 cut(s) 26, 276, 521
BsiEI CGRYCG 1 cut(s) 116
BsiSI CCGG 3 cut(s) 27, 225, 278
BslFI GGGAC 2 cut(s) 32, 557
BslI CCNNNNNNNGG 1 cut(s) 594
BsmFI GGGAC 2 cut(s) 32, 557
BsnI GGCC 3 cut(s) 26, 276, 521
Bsp1407I TGTACA 3 cut(s) 3, 294, 315
Bsp143I GATC 4 cut(s) 34, 108, 113, 468
Bsp19I CCATGG 1 cut(s) 211
BspACI CCGC 2 cut(s) 588, 595
BspANI GGCC 3 cut(s) 26, 276, 521
BspCNI CTCAG 2 cut(s) 226, 253
BspLI GGNNCC 2 cut(s) 20, 573
BspPI GGATC 2 cut(s) 42, 103
BspTI CTTAAG 1 cut(s) 38
BsrFI RCCGGY 1 cut(s) 26
BsrGI TGTACA 3 cut(s) 3, 294, 315
BsrI ACTGG 2 cut(s) 44, 573
BssAI RCCGGY 1 cut(s) 26
BssECI CCNNGG 1 cut(s) 211
BssMI GATC 4 cut(s) 34, 108, 113, 468
BssT1I CCWWGG 1 cut(s) 211
Bst2UI CCWGG 2 cut(s) 23, 592
Bst4CI ACNGT 1 cut(s) 457
BstAFI CTTAAG 1 cut(s) 38
BstAUI TGTACA 3 cut(s) 3, 294, 315
BstC8I GCNNGC 3 cut(s) 28, 354, 419
BstDEI CTNAG 2 cut(s) 234, 261
BstDSI CCRYGG 1 cut(s) 211
BstF5I GGATG 2 cut(s) 262, 469
BstKTI GATC 4 cut(s) 37, 111, 116, 471
BstMBI GATC 4 cut(s) 34, 108, 113, 468
BstMCI CGRYCG 1 cut(s) 116
BstMWI GCNNNNNNNGC 3 cut(s) 142, 358, 557
BstNI CCWGG 2 cut(s) 23, 592
BstSCI CCNGG 3 cut(s) 21, 276, 590
BstV1I GCAGC 2 cut(s) 216, 560
BstX2I RGATCY 1 cut(s) 34
BstXI CCANNNNNNTGG 1 cut(s) 568
BstYI RGATCY 1 cut(s) 34
BsuI GTATCC 1 cut(s) 274
BsuRI GGCC 3 cut(s) 26, 276, 521
BtgI CCRYGG 1 cut(s) 211
BtrI CACGTC 1 cut(s) 427
BtsCI GGATG 2 cut(s) 262, 469
BtsIMutI CAGTG 2 cut(s) 72, 566
Cac8I GCNNGC 3 cut(s) 28, 354, 419
Cfr10I RCCGGY 1 cut(s) 26
Cfr13I GGNCC 3 cut(s) 19, 275, 571
CseI GACGC 1 cut(s) 196
Csp6I GTAC 3 cut(s) 4, 295, 316
CviAII CATG 2 cut(s) 212, 358
CviQI GTAC 3 cut(s) 4, 295, 316
DdeI CTNAG 2 cut(s) 234, 261
DpnI GATC 4 cut(s) 36, 110, 115, 470
DpnII GATC 4 cut(s) 34, 108, 113, 468
EciI GGCGGA 1 cut(s) 577
Eco130I CCWWGG 1 cut(s) 211
Eco47I GGWCC 2 cut(s) 19, 571
Eco57I CTGAAG 1 cut(s) 528
EcoRII CCWGG 2 cut(s) 21, 590
EcoT14I CCWWGG 1 cut(s) 211
EcoT22I ATGCAT 1 cut(s) 453
ErhI CCWWGG 1 cut(s) 211
FaeI CATG 2 cut(s) 215, 361
FaiI YATR 8 cut(s) 8, 200, 213, 359, 371, 415, 453, 515
FalI AAGNNNNNCTT 2 cut(s) 74, 106
FaqI GGGAC 2 cut(s) 32, 557
FatI CATG 2 cut(s) 211, 357
Fnu4HI GCNGC 2 cut(s) 205, 549
FokI GGATG 2 cut(s) 269, 476
Fsp4HI GCNGC 2 cut(s) 205, 549
GluI GCNGC 2 cut(s) 205, 549
GsuI CTGGAG 1 cut(s) 119
HaeIII GGCC 3 cut(s) 26, 276, 521
HapII CCGG 3 cut(s) 27, 225, 278
HgaI GACGC 1 cut(s) 196
Hin1II CATG 2 cut(s) 215, 361
HinfI GANTC 2 cut(s) 263, 598
HpaII CCGG 3 cut(s) 27, 225, 278
HphI GGTGA 2 cut(s) 130, 136
Hpy166II GTNNAC 1 cut(s) 295
Hpy188I TCNGA 2 cut(s) 113, 343
Hpy188III TCNNGA 1 cut(s) 98
Hpy8I GTNNAC 1 cut(s) 295
Hpy99I CGWCG 1 cut(s) 527
HpyAV CCTTC 1 cut(s) 511
HpyCH4III ACNGT 1 cut(s) 457
HpyCH4IV ACGT 1 cut(s) 426
HpyCH4V TGCA 4 cut(s) 59, 66, 308, 451
HpyF10VI GCNNNNNNNGC 3 cut(s) 142, 358, 557
HpyF3I CTNAG 2 cut(s) 234, 261
HpySE526I ACGT 1 cut(s) 426
Hsp92II CATG 2 cut(s) 215, 361
KroI GCCGGC 1 cut(s) 26
KroNI GCCGGC 1 cut(s) 28
Kzo9I GATC 4 cut(s) 34, 108, 113, 468
Lsp1109I GCAGC 2 cut(s) 216, 560
LweI GCATC 2 cut(s) 247, 438
MaeII ACGT 1 cut(s) 426
MalI GATC 4 cut(s) 36, 110, 115, 470
MboI GATC 4 cut(s) 34, 108, 113, 468
MboII GAAGA 2 cut(s) 115, 539
MflI RGATCY 1 cut(s) 34
MluCI AATT 5 cut(s) 9, 68, 167, 247, 436
MlyI GAGTC 1 cut(s) 272
MnlI CCTC 4 cut(s) 156, 377, 473, 562
Mph1103I ATGCAT 1 cut(s) 453
MroNI GCCGGC 1 cut(s) 26
MseI TTAA 1 cut(s) 39
MspCI CTTAAG 1 cut(s) 38
MspI CCGG 3 cut(s) 27, 225, 278
MspR9I CCNGG 3 cut(s) 23, 278, 592
MvaI CCWGG 2 cut(s) 23, 592
MwoI GCNNNNNNNGC 3 cut(s) 142, 358, 557
NaeI GCCGGC 1 cut(s) 28
NciI CCSGG 1 cut(s) 278
NcoI CCATGG 1 cut(s) 211
NdeII GATC 4 cut(s) 34, 108, 113, 468
NgoMIV GCCGGC 1 cut(s) 26
NlaIII CATG 2 cut(s) 215, 361
NlaIV GGNNCC 2 cut(s) 20, 573
NsiI ATGCAT 1 cut(s) 453
PdiI GCCGGC 1 cut(s) 28
PkrI GCNGC 2 cut(s) 206, 550
Ple19I CGATCG 1 cut(s) 116
PleI GAGTC 1 cut(s) 271
PpsI GAGTC 1 cut(s) 271
PsiI TTATAA 1 cut(s) 200
Psp6I CCWGG 2 cut(s) 21, 590
PspGI CCWGG 2 cut(s) 21, 590
PspN4I GGNNCC 2 cut(s) 20, 573
PspPI GGNCC 3 cut(s) 19, 275, 571
PsuI RGATCY 1 cut(s) 34
PvuI CGATCG 1 cut(s) 116
RsaI GTAC 3 cut(s) 5, 296, 317
RsaNI GTAC 3 cut(s) 4, 295, 316
SaqAI TTAA 1 cut(s) 39
SatI GCNGC 2 cut(s) 205, 549
Sau3AI GATC 4 cut(s) 34, 108, 113, 468
Sau96I GGNCC 3 cut(s) 19, 275, 571
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 3 cut(s) 23, 278, 592
SetI ASST 8 cut(s) 235, 341, 354, 369, 423, 429, 484, 542
SfaNI GCATC 2 cut(s) 247, 438
SinI GGWCC 2 cut(s) 19, 571
SmlI CTYRAG 2 cut(s) 38, 535
SmoI CTYRAG 2 cut(s) 38, 535
Sse9I AATT 5 cut(s) 9, 68, 167, 247, 436
SsiI CCGC 2 cut(s) 588, 595
StyD4I CCNGG 3 cut(s) 21, 276, 590
StyI CCWWGG 1 cut(s) 211
TaaI ACNGT 1 cut(s) 457
TaiI ACGT 1 cut(s) 429
TaqI TCGA 4 cut(s) 333, 363, 525, 601
TasI AATT 5 cut(s) 9, 68, 167, 247, 436
TatI WGTACW 3 cut(s) 3, 294, 315
Tru1I TTAA 1 cut(s) 39
Tru9I TTAA 1 cut(s) 39
TscAI CASTG 2 cut(s) 79, 573
TseI GCWGC 2 cut(s) 204, 548
TspDTI ATGAA 1 cut(s) 530
TspRI CASTG 2 cut(s) 79, 573
Vha464I CTTAAG 1 cut(s) 38
VpaK11BI GGWCC 2 cut(s) 19, 571
XapI RAATTY 2 cut(s) 68, 167
Zsp2I ATGCAT 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.