AT3G52210

mRNA cap guanine-N7 methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007883
Physical Location & Seq
Forward (+)
19366984 .. 19369494
2511 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G52210.1

Sequence Viewer

Length: 1065 bp
ATGAGCGGCTTCGTGGTTTCAAAACCGGAGCAGAGCCATCATCGTCTCTTCGATTTCGCAAAGACGGCCATAATCAACATCTTCGCACATCCTTATGCCACTGTTTGCGAATTGTACTGTGGCGGAGCTCCCGAAACCGACAAGTGGGAAGCTGCTCCGATTGGTCACTACATCGGAATTGATACATCTTCTGGGATTTCTTCTGTACGAGAAGCTTGGGAGAGTCAGAGGAAGAATTACGATGTGGAATTCTTTGAAGCTGATCCTTCCAAGGATGATTTTGAAATCCAGCTACAGAAGAAATTGGAGCAGGCTGATTTAGTTTCTTGTTGGCGCCATCTCCAGTTATGCTTTGAAACTGAGGAGAGTGCGAGAAGACTCCTAACTAATGTAGCATGTTTACTGAAACCAGGGGGTTATTTTTTCGGTATCACTCCTGACTCGTCTACGATTTGGGCAAAGTACCAGAAAAATGTTGAAGCATACCACAACAGAAGCGGTGCAAAGCCTAATGTATTTCCAAACTACATTCGGTCTGAAAGTTACATGATCACTTTCGAATTGGAGGAAGAAAAATTTCCGCTGTTTGGAAAGAGGTACCAGCTGAAATTTTCTGGTGACAATGCTTCTGAAGACCATTGCTTGGTTCACTTCCCAAGCTTAATCAGGTTAGCGAGGGAAGCTGGCCTTGAATTTGTGGAAATTCAAAGTTTAACAGATTTTTATGATGATAACAGAGCCCAGTTTGCAAGCTTGCTGATGAATGCTGGCCCGAATTTCGTTGACCCTAGGGGAAAACTTCTTCCAAGAGCATTTGATTTGTTAGGTCTGTATGCAACATTCATATTTCAGAAGCCTGACCCAGATATCGAACCTCCTCTAACGACCCCAATACCTTTTGAAAGCTCCAATAATCATGATGAGAGAGAATTGCCAGTTATCACGGTGATCACTGACGCAAGTGCACCAGCAGAAGATCCATCACAAGGACTAGGGAAGATTGTTGAACAAAAAGGAATACTGGGACCAGGCCCTGCTGATTTACGTTTCTCTGAAGCAATTTGA

Protein Analysis

354

Amino Acids

39.89

Weight (kDa)

4.95

Isoelectric Point (pI)

51.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
mRNA_G-N7_MeTrfase PF03291 21 - 285 2.4e-31 mRNA (guanine-N(7))-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 597
AccB1I GGYRCC 2 cut(s) 333, 597
AccB7I CCANNNNNTGG 1 cut(s) 643
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 1 cut(s) 446
AciI CCGC 4 cut(s) 6, 123, 498, 581
AclWI GGATC 2 cut(s) 257, 971
AcoI YGGCCR 1 cut(s) 66
AcsI RAATTY 6 cut(s) 248, 575, 608, 692, 702, 775
AcuI CTGAAG 1 cut(s) 651
AcyI GRCGYC 1 cut(s) 334
AfaI GTAC 4 cut(s) 116, 207, 464, 599
AfiI CCNNNNNNNGG 4 cut(s) 144, 587, 643, 986
AgsI TTSAA 9 cut(s) 21, 257, 284, 356, 479, 692, 707, 902, 1007
AjnI CCWGG 2 cut(s) 409, 1027
Alw21I GWGCWC 2 cut(s) 130, 967
Alw26I GTCTC 1 cut(s) 50
Alw44I GTGCAC 1 cut(s) 963
AlwI GGATC 2 cut(s) 257, 971
AlwNI CAGNNNCTG 1 cut(s) 1034
AoxI GGCC 4 cut(s) 66, 685, 769, 1030
ApaLI GTGCAC 1 cut(s) 963
ApeKI GCWGC 1 cut(s) 152
ApoI RAATTY 6 cut(s) 248, 575, 608, 692, 702, 775
Asp700I GAANNNNTTC 1 cut(s) 576
Asp718I GGTACC 1 cut(s) 597
AspA2I CCTAGG 1 cut(s) 788
AspLEI GCGC 1 cut(s) 336
AspS9I GGNCC 3 cut(s) 770, 1025, 1031
AsuHPI GGTGA 2 cut(s) 629, 958
AsuII TTCGAA 1 cut(s) 558
AvaII GGWCC 1 cut(s) 1025
AvrII CCTAGG 1 cut(s) 788
BaeGI GKGCMC 1 cut(s) 967
BanI GGYRCC 2 cut(s) 333, 597
BanII GRGCYC 2 cut(s) 130, 742
BbsI GAAGAC 2 cut(s) 382, 639
Bbv12I GWGCWC 2 cut(s) 130, 967
BbvI GCAGC 1 cut(s) 139
BccI CCATC 3 cut(s) 45, 345, 988
BceAI ACGGC 1 cut(s) 81
BciT130I CCWGG 2 cut(s) 411, 1029
BclI TGATCA 2 cut(s) 549, 948
BcoDI GTCTC 1 cut(s) 50
BfaI CTAG 2 cut(s) 789, 992
BfmI CTRYAG 1 cut(s) 293
BfoI RGCGCY 1 cut(s) 337
BisI GCNGC 2 cut(s) 7, 153
BlnI CCTAGG 1 cut(s) 788
BlsI GCNGC 2 cut(s) 8, 154
Bme1390I CCNGG 2 cut(s) 411, 1029
Bme18I GGWCC 1 cut(s) 1025
BmgT120I GGNCC 3 cut(s) 770, 1025, 1031
BmiI GGNNCC 3 cut(s) 335, 599, 1026
BmrFI CCNGG 2 cut(s) 411, 1029
BmrI ACTGGG 2 cut(s) 736, 1031
BmuI ACTGGG 2 cut(s) 736, 1031
BpiI GAAGAC 2 cut(s) 382, 639
BpmI CTGGAG 1 cut(s) 326
Bpu14I TTCGAA 1 cut(s) 558
BsaHI GRCGYC 1 cut(s) 334
BsaJI CCNNGG 3 cut(s) 270, 410, 788
BsaWI WCCGGW 1 cut(s) 25
Bsc4I CCNNNNNNNGG 4 cut(s) 144, 587, 643, 986
Bse1I ACTGG 4 cut(s) 343, 742, 935, 1026
Bse3DI GCAATG 1 cut(s) 637
BseBI CCWGG 2 cut(s) 411, 1029
BseDI CCNNGG 3 cut(s) 270, 410, 788
BseGI GGATG 2 cut(s) 88, 280
BseLI CCNNNNNNNGG 4 cut(s) 144, 587, 643, 986
BseMI GCAATG 1 cut(s) 637
BseMII CTCAG 1 cut(s) 351
BseNI ACTGG 4 cut(s) 343, 742, 935, 1026
BseRI GAGGAG 2 cut(s) 377, 867
BseSI GKGCMC 1 cut(s) 967
BseXI GCAGC 1 cut(s) 139
BshFI GGCC 4 cut(s) 68, 687, 771, 1032
BshNI GGYRCC 2 cut(s) 333, 597
BsiHKAI GWGCWC 2 cut(s) 130, 967
BsiSI CCGG 1 cut(s) 26
BslFI GGGAC 1 cut(s) 1038
BslI CCNNNNNNNGG 4 cut(s) 144, 587, 643, 986
BsmAI GTCTC 1 cut(s) 50
BsmBI CGTCTC 1 cut(s) 50
BsmFI GGGAC 1 cut(s) 1038
BsmI GAATGC 1 cut(s) 769
BsnI GGCC 4 cut(s) 68, 687, 771, 1032
Bsp119I TTCGAA 1 cut(s) 558
Bsp1286I GDGCHC 3 cut(s) 130, 742, 967
Bsp143I GATC 4 cut(s) 262, 549, 948, 976
BspACI CCGC 4 cut(s) 6, 123, 498, 581
BspANI GGCC 4 cut(s) 68, 687, 771, 1032
BspCNI CTCAG 1 cut(s) 352
BspHI TCATGA 1 cut(s) 916
BspLI GGNNCC 3 cut(s) 335, 599, 1026
BspPI GGATC 2 cut(s) 257, 971
BspT104I TTCGAA 1 cut(s) 558
BspT107I GGYRCC 2 cut(s) 333, 597
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 1 cut(s) 637
BsrI ACTGG 4 cut(s) 343, 742, 935, 1026
BssECI CCNNGG 3 cut(s) 270, 410, 788
BssMI GATC 4 cut(s) 262, 549, 948, 976
BssNI GRCGYC 1 cut(s) 334
BssT1I CCWWGG 2 cut(s) 270, 788
Bst2UI CCWGG 2 cut(s) 411, 1029
Bst4CI ACNGT 3 cut(s) 103, 119, 946
Bst6I CTCTTC 1 cut(s) 53
BstACI GRCGYC 1 cut(s) 334
BstBI TTCGAA 1 cut(s) 558
BstC8I GCNNGC 5 cut(s) 312, 685, 751, 755, 769
BstDEI CTNAG 1 cut(s) 360
BstF5I GGATG 2 cut(s) 88, 280
BstH2I RGCGCY 1 cut(s) 337
BstHHI GCGC 1 cut(s) 336
BstKTI GATC 4 cut(s) 265, 552, 951, 979
BstMAI GTCTC 1 cut(s) 50
BstMBI GATC 4 cut(s) 262, 549, 948, 976
BstMWI GCNNNNNNNGC 3 cut(s) 65, 680, 746
BstNI CCWGG 2 cut(s) 411, 1029
BstNSI RCATGY 1 cut(s) 399
BstSCI CCNGG 2 cut(s) 409, 1027
BstSFI CTRYAG 1 cut(s) 293
BstSLI GKGCMC 1 cut(s) 967
BstV1I GCAGC 1 cut(s) 139
BstV2I GAAGAC 2 cut(s) 382, 639
BstX2I RGATCY 1 cut(s) 976
BstYI RGATCY 1 cut(s) 976
BsuRI GGCC 4 cut(s) 68, 687, 771, 1032
BtsCI GGATG 2 cut(s) 88, 280
BtsIMutI CAGTG 2 cut(s) 99, 951
Cac8I GCNNGC 5 cut(s) 312, 685, 751, 755, 769
CaiI CAGNNNCTG 1 cut(s) 1034
CciI TCATGA 1 cut(s) 916
CfoI GCGC 1 cut(s) 336
Cfr13I GGNCC 3 cut(s) 770, 1025, 1031
CseI GACGC 1 cut(s) 965
Csp6I GTAC 4 cut(s) 115, 206, 463, 598
CviAII CATG 3 cut(s) 396, 547, 917
CviQI GTAC 4 cut(s) 115, 206, 463, 598
DdeI CTNAG 1 cut(s) 360
DinI GGCGCC 1 cut(s) 335
DpnI GATC 4 cut(s) 264, 551, 950, 978
DpnII GATC 4 cut(s) 262, 549, 948, 976
EaeI YGGCCR 1 cut(s) 66
Eam1104I CTCTTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 53
EciI GGCGGA 1 cut(s) 138
Ecl136II GAGCTC 1 cut(s) 128
Eco130I CCWWGG 2 cut(s) 270, 788
Eco24I GRGCYC 2 cut(s) 130, 742
Eco32I GATATC 1 cut(s) 868
Eco47I GGWCC 1 cut(s) 1025
Eco53kI GAGCTC 1 cut(s) 128
Eco57I CTGAAG 1 cut(s) 651
EcoICRI GAGCTC 1 cut(s) 128
EcoO109I RGGNCCY 1 cut(s) 1031
EcoRI GAATTC 1 cut(s) 248
EcoRII CCWGG 2 cut(s) 409, 1027
EcoRV GATATC 1 cut(s) 868
EcoT14I CCWWGG 2 cut(s) 270, 788
EcoT38I GRGCYC 2 cut(s) 130, 742
EgeI GGCGCC 1 cut(s) 335
EheI GGCGCC 1 cut(s) 335
ErhI CCWWGG 2 cut(s) 270, 788
Esp3I CGTCTC 1 cut(s) 50
FaeI CATG 3 cut(s) 399, 550, 920
FalI AAGNNNNNCTT 2 cut(s) 672, 704
FaqI GGGAC 1 cut(s) 1038
FatI CATG 3 cut(s) 395, 546, 916
FbaI TGATCA 2 cut(s) 549, 948
FblI GTMKAC 1 cut(s) 446
Fnu4HI GCNGC 2 cut(s) 7, 153
FokI GGATG 2 cut(s) 75, 287
FriOI GRGCYC 2 cut(s) 130, 742
Fsp4HI GCNGC 2 cut(s) 7, 153
FspBI CTAG 2 cut(s) 789, 992
GlaI GCGC 1 cut(s) 335
GluI GCNGC 2 cut(s) 7, 153
GsuI CTGGAG 1 cut(s) 326
HaeII RGCGCY 1 cut(s) 337
HaeIII GGCC 4 cut(s) 68, 687, 771, 1032
HapII CCGG 1 cut(s) 26
HgaI GACGC 1 cut(s) 965
HhaI GCGC 1 cut(s) 336
Hin1I GRCGYC 1 cut(s) 334
Hin1II CATG 3 cut(s) 399, 550, 920
Hin6I GCGC 1 cut(s) 334
HinP1I GCGC 1 cut(s) 334
HincII GTYRAC 1 cut(s) 784
HindII GTYRAC 1 cut(s) 784
HindIII AAGCTT 3 cut(s) 213, 658, 751
HinfI GANTC 3 cut(s) 223, 378, 440
HpaII CCGG 1 cut(s) 26
HphI GGTGA 2 cut(s) 629, 958
Hpy166II GTNNAC 5 cut(s) 401, 447, 649, 784, 965
Hpy188I TCNGA 7 cut(s) 159, 176, 228, 538, 631, 852, 1054
Hpy188III TCNNGA 3 cut(s) 131, 437, 917
Hpy8I GTNNAC 5 cut(s) 401, 447, 649, 784, 965
HpyAV CCTTC 1 cut(s) 276
HpyCH4III ACNGT 3 cut(s) 103, 119, 946
HpyCH4IV ACGT 1 cut(s) 1045
HpyCH4V TGCA 4 cut(s) 503, 749, 836, 965
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 680, 746
HpyF3I CTNAG 1 cut(s) 360
HpySE526I ACGT 1 cut(s) 1045
Hsp92I GRCGYC 1 cut(s) 334
Hsp92II CATG 3 cut(s) 399, 550, 920
HspAI GCGC 1 cut(s) 334
KasI GGCGCC 1 cut(s) 333
KpnI GGTACC 1 cut(s) 601
Ksp22I TGATCA 2 cut(s) 549, 948
Kzo9I GATC 4 cut(s) 262, 549, 948, 976
LmnI GCTCC 6 cut(s) 28, 125, 133, 160, 307, 911
Lsp1109I GCAGC 1 cut(s) 139
MaeI CTAG 2 cut(s) 789, 992
MaeII ACGT 1 cut(s) 1045
MaeIII GTNAC 3 cut(s) 164, 542, 617
MalI GATC 4 cut(s) 264, 551, 950, 978
MbiI CCGCTC 1 cut(s) 6
MboI GATC 4 cut(s) 262, 549, 948, 976
MflI RGATCY 1 cut(s) 976
MhlI GDGCHC 3 cut(s) 130, 742, 967
Mly113I GGCGCC 1 cut(s) 334
MlyI GAGTC 3 cut(s) 232, 372, 434
MnlI CCTC 7 cut(s) 222, 355, 559, 588, 669, 885, 888
MroXI GAANNNNTTC 1 cut(s) 576
MseI TTAA 2 cut(s) 662, 713
MslI CAYNNNNRTG 1 cut(s) 93
MspA1I CMGCKG 2 cut(s) 583, 604
MspI CCGG 1 cut(s) 26
MspR9I CCNGG 2 cut(s) 411, 1029
Mva1269I GAATGC 1 cut(s) 769
MvaI CCWGG 2 cut(s) 411, 1029
MwoI GCNNNNNNNGC 3 cut(s) 65, 680, 746
NarI GGCGCC 1 cut(s) 334
NdeII GATC 4 cut(s) 262, 549, 948, 976
NlaIII CATG 3 cut(s) 399, 550, 920
NlaIV GGNNCC 3 cut(s) 335, 599, 1026
NmuCI GTSAC 2 cut(s) 164, 617
NspI RCATGY 1 cut(s) 399
NspV TTCGAA 1 cut(s) 558
PagI TCATGA 1 cut(s) 916
PctI GAATGC 1 cut(s) 769
PdmI GAANNNNTTC 1 cut(s) 576
PflMI CCANNNNNTGG 1 cut(s) 643
PkrI GCNGC 2 cut(s) 8, 154
PleI GAGTC 3 cut(s) 231, 372, 434
PluTI GGCGCC 1 cut(s) 337
PpsI GAGTC 3 cut(s) 231, 372, 434
Psp124BI GAGCTC 1 cut(s) 130
Psp6I CCWGG 2 cut(s) 409, 1027
PspGI CCWGG 2 cut(s) 409, 1027
PspN4I GGNNCC 3 cut(s) 335, 599, 1026
PspPI GGNCC 3 cut(s) 770, 1025, 1031
PstNI CAGNNNCTG 1 cut(s) 1034
PsuI RGATCY 1 cut(s) 976
PvuII CAGCTG 1 cut(s) 604
RsaI GTAC 4 cut(s) 116, 207, 464, 599
RsaNI GTAC 4 cut(s) 115, 206, 463, 598
RseI CAYNNNNRTG 1 cut(s) 93
SacI GAGCTC 1 cut(s) 130
SaqAI TTAA 2 cut(s) 662, 713
SatI GCNGC 2 cut(s) 7, 153
Sau3AI GATC 4 cut(s) 262, 549, 948, 976
Sau96I GGNCC 3 cut(s) 770, 1025, 1031
SchI GAGTC 3 cut(s) 232, 372, 434
ScrFI CCNGG 2 cut(s) 411, 1029
SduI GDGCHC 3 cut(s) 130, 742, 967
SfcI CTRYAG 1 cut(s) 293
SfoI GGCGCC 1 cut(s) 335
SfuI TTCGAA 1 cut(s) 558
SinI GGWCC 1 cut(s) 1025
SmiMI CAYNNNNRTG 1 cut(s) 93
SsiI CCGC 4 cut(s) 6, 123, 498, 581
SspDI GGCGCC 1 cut(s) 333
SspMI CTAG 2 cut(s) 789, 992
SstI GAGCTC 1 cut(s) 130
StyD4I CCNGG 2 cut(s) 409, 1027
StyI CCWWGG 2 cut(s) 270, 788
TaaI ACNGT 3 cut(s) 103, 119, 946
TaiI ACGT 1 cut(s) 1048
TaqI TCGA 3 cut(s) 51, 558, 870
TaqII GACCGA 1 cut(s) 522
TatI WGTACW 1 cut(s) 114
TauI GCSGC 1 cut(s) 9
Tru1I TTAA 2 cut(s) 662, 713
Tru9I TTAA 2 cut(s) 662, 713
TscAI CASTG 2 cut(s) 106, 958
TseFI GTSAC 2 cut(s) 164, 617
TseI GCWGC 1 cut(s) 152
Tsp45I GTSAC 2 cut(s) 164, 617
TspDTI ATGAA 2 cut(s) 776, 832
TspRI CASTG 2 cut(s) 106, 958
Van91I CCANNNNNTGG 1 cut(s) 643
VneI GTGCAC 1 cut(s) 963
VpaK11BI GGWCC 1 cut(s) 1025
XapI RAATTY 6 cut(s) 248, 575, 608, 692, 702, 775
XceI RCATGY 1 cut(s) 399
XmaJI CCTAGG 1 cut(s) 788
XmiI GTMKAC 1 cut(s) 446
XmnI GAANNNNTTC 1 cut(s) 576
XspI CTAG 2 cut(s) 789, 992
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.