AT3G53010

carbohydrate esterase At4g34215

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0011720
Physical Location & Seq
Reverse (-)
19656488 .. 19658124
1637 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G53010.1

Sequence Viewer

Length: 894 bp
ATGACAAAGATCTTCTACTACTTCTTCATCATCATTTTTCTTCAATCTCCACCGCATCTTCAATCCCAAACCATCACAAGGAACATCAGCATTTTCATCTTGGCCGGGCAAAGCAACATGGCGGGTCGAGGCGGTGTTTATAACGACACTGCCACAAACACCACCGTGTGGGACGGTGTAATTCCGCCAGAATGCCGATCAAACCCTTCGATCCTCCGTCTCACGAGTAAACTCGAGTGGAAAGAGGCTAAAGAGCCACTTCACGTTGACATTGACATTAATAAGACTAATGGTGTTGGACCGGGGATGCCCTTTGCTAACCGGGTGGTTAACCGGTTTGGTCAGGTGGGTTTGGTTCCGTGCTCTATCGGTGGGACTAAACTAAGCCAATGGCAAAAAGGTGAGTTTCTTTACGAGGAGACGGTGAAGAGGGCTAAAGCGGCGATGGCTAGTGGTGGTGGTGGCTCGTACCGGGCGGTTTTGTGGTATCAGGGTGAGTCTGATACGGTTGATATGGTCGATGCTTCGGTATATAAGAAGAGACTAGTGAAGTTTTTCAGTGATCTTCGAAATGATTTACAACACCCAAATCTTCCTATTATCCAGGTGGCTCTAGCGACAGGAGCAGGACCATATCTTGACGCGGTGAGAAAAGCTCAACTGAAGACTGATCTTGAGAACGTGTATTGCGTTGACGCCAGAGGCCTACCTCTTGAACCGGACGGTTTGCATCTGACCACTTCCTCTCAGGTCCAGCTCGGTCACATGATAGCCGAGAGTTTCTTGGCTATTCCAAATTCAGCTCGACCATCACTCTACATGCATTTTTTCTCCTTGTTCTGTTCCCTTTATGTTTCTTTAAATATTTTTGGAACTATCTTTCATTTTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

297

Amino Acids

32.96

Weight (kDa)

8.59

Isoelectric Point (pI)

40.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 30 - 262 3.8e-89 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011720)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53010
fragaria_vesca FvH4_2g33800
malus_domestica MD08G1092200.v1.1 MD15G1076700.v1.1
prunus_persica Prupe.1G430100_v2.0.a1
pyrus_communis pycom08g07490 pycom08g07500 pycom15g07150
rosa_chinensis RchiOBHm_Chr6g0308761
rosa_laevigata RLG00000010622
rosa_multiflora Rmu_sc0000871.1_g000025 Rmu_sc0009346.1_g000012
rosa_roxburghii Rroxscaffold_7G00159960
rosa_rugosa Rorug06G0368100
rosa_samantha Rh6AG479200 Rh6BG488800 Rh6CG494000 Rh6DG479600
rosa_wichuraiana Rw6G041770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 141
AccB7I CCANNNNNTGG 1 cut(s) 168
AccII CGCG 1 cut(s) 644
AciI CCGC 7 cut(s) 53, 122, 132, 185, 440, 476, 644
AclWI GGATC 1 cut(s) 205
AcoI YGGCCR 1 cut(s) 102
AcsI RAATTY 1 cut(s) 796
AcuI CTGAAG 1 cut(s) 683
AcyI GRCGYC 1 cut(s) 696
AdeI CACNNNGTG 1 cut(s) 168
AfaI GTAC 1 cut(s) 470
AfiI CCNNNNNNNGG 2 cut(s) 78, 168
AflIII ACRYGT 1 cut(s) 681
AgeI ACCGGT 1 cut(s) 333
AgsI TTSAA 3 cut(s) 44, 62, 716
AhlI ACTAGT 1 cut(s) 544
AjnI CCWGG 1 cut(s) 603
AleI CACNNNNGTG 1 cut(s) 164
AluBI AGCT 3 cut(s) 656, 757, 803
AluI AGCT 3 cut(s) 656, 757, 803
Alw21I GWGCWC 1 cut(s) 365
Alw26I GTCTC 3 cut(s) 224, 413, 535
AlwI GGATC 1 cut(s) 205
Ama87I CYCGRG 1 cut(s) 233
AoxI GGCC 2 cut(s) 102, 703
ApoI RAATTY 1 cut(s) 796
AseI ATTAAT 1 cut(s) 279
AsiGI ACCGGT 1 cut(s) 333
Asp700I GAANNNNTTC 1 cut(s) 554
AspS9I GGNCC 3 cut(s) 299, 629, 751
AsuC2I CCSGG 4 cut(s) 106, 303, 323, 473
AsuHPI GGTGA 4 cut(s) 413, 436, 506, 658
AsuII TTCGAA 1 cut(s) 568
AvaI CYCGRG 1 cut(s) 233
AvaII GGWCC 3 cut(s) 299, 629, 751
BauI CACGAG 1 cut(s) 223
BbsI GAAGAC 1 cut(s) 671
Bbv12I GWGCWC 1 cut(s) 365
BccI CCATC 3 cut(s) 80, 439, 817
BciT130I CCWGG 1 cut(s) 605
BcnI CCSGG 4 cut(s) 106, 303, 323, 473
BcoDI GTCTC 3 cut(s) 224, 413, 535
BcuI ACTAGT 1 cut(s) 544
BfaI CTAG 3 cut(s) 450, 545, 614
BglII AGATCT 1 cut(s) 9
BisI GCNGC 1 cut(s) 441
BlsI GCNGC 1 cut(s) 442
Bme1390I CCNGG 5 cut(s) 106, 303, 323, 473, 605
Bme18I GGWCC 3 cut(s) 299, 629, 751
BmeT110I CYCGRG 1 cut(s) 233
BmgT120I GGNCC 3 cut(s) 299, 629, 751
BmiI GGNNCC 1 cut(s) 357
BmrFI CCNGG 5 cut(s) 106, 303, 323, 473, 605
BmsI GCATC 4 cut(s) 64, 297, 511, 739
BpiI GAAGAC 1 cut(s) 671
BplI GAGNNNNNCTC 2 cut(s) 640, 672
Bpu14I TTCGAA 1 cut(s) 568
BpuEI CTTGAG 1 cut(s) 695
BpuMI CCSGG 4 cut(s) 106, 303, 323, 473
BsaHI GRCGYC 1 cut(s) 696
BsaJI CCNNGG 1 cut(s) 302
BsaWI WCCGGW 2 cut(s) 333, 718
Bsc4I CCNNNNNNNGG 2 cut(s) 78, 168
Bse118I RCCGGY 1 cut(s) 333
BseBI CCWGG 1 cut(s) 605
BseDI CCNNGG 1 cut(s) 302
BseGI GGATG 1 cut(s) 312
BseLI CCNNNNNNNGG 2 cut(s) 78, 168
BseMII CTCAG 1 cut(s) 761
BseRI GAGGAG 1 cut(s) 431
Bsh1236I CGCG 1 cut(s) 644
BshFI GGCC 2 cut(s) 104, 705
BshTI ACCGGT 1 cut(s) 333
BsiHKAI GWGCWC 1 cut(s) 365
BsiHKCI CYCGRG 1 cut(s) 233
BsiSI CCGG 6 cut(s) 105, 302, 322, 334, 472, 719
BslFI GGGAC 2 cut(s) 185, 388
BslI CCNNNNNNNGG 2 cut(s) 78, 168
BsmAI GTCTC 3 cut(s) 224, 413, 535
BsmBI CGTCTC 2 cut(s) 224, 413
BsmFI GGGAC 2 cut(s) 185, 388
BsmI GAATGC 1 cut(s) 197
BsnI GGCC 2 cut(s) 104, 705
BsoBI CYCGRG 1 cut(s) 233
Bsp119I TTCGAA 1 cut(s) 568
Bsp1286I GDGCHC 1 cut(s) 365
Bsp143I GATC 5 cut(s) 9, 197, 210, 562, 670
BspACI CCGC 7 cut(s) 53, 122, 132, 185, 440, 476, 644
BspANI GGCC 2 cut(s) 104, 705
BspCNI CTCAG 1 cut(s) 760
BspFNI CGCG 1 cut(s) 644
BspLI GGNNCC 1 cut(s) 357
BspPI GGATC 1 cut(s) 205
BspT104I TTCGAA 1 cut(s) 568
BsrFI RCCGGY 1 cut(s) 333
BssAI RCCGGY 1 cut(s) 333
BssECI CCNNGG 1 cut(s) 302
BssMI GATC 5 cut(s) 9, 197, 210, 562, 670
BssNI GRCGYC 1 cut(s) 696
BssSI CACGAG 1 cut(s) 223
Bst2BI CACGAG 1 cut(s) 223
Bst2UI CCWGG 1 cut(s) 605
Bst4CI ACNGT 5 cut(s) 166, 176, 424, 508, 725
Bst6I CTCTTC 2 cut(s) 422, 533
BstACI GRCGYC 1 cut(s) 696
BstBI TTCGAA 1 cut(s) 568
BstDEI CTNAG 2 cut(s) 383, 747
BstF5I GGATG 1 cut(s) 312
BstFNI CGCG 1 cut(s) 644
BstKTI GATC 5 cut(s) 12, 200, 213, 565, 673
BstMAI GTCTC 3 cut(s) 224, 413, 535
BstMBI GATC 5 cut(s) 9, 197, 210, 562, 670
BstMWI GCNNNNNNNGC 3 cut(s) 440, 446, 623
BstNI CCWGG 1 cut(s) 605
BstNSI RCATGY 1 cut(s) 823
BstSCI CCNGG 5 cut(s) 104, 301, 321, 471, 603
BstUI CGCG 1 cut(s) 644
BstV2I GAAGAC 1 cut(s) 671
BstX2I RGATCY 1 cut(s) 9
BstYI RGATCY 1 cut(s) 9
BsuRI GGCC 2 cut(s) 104, 705
BtgZI GCGATG 1 cut(s) 458
BtsCI GGATG 1 cut(s) 312
BtsI GCAGTG 1 cut(s) 147
BtsIMutI CAGTG 2 cut(s) 147, 565
Cfr10I RCCGGY 1 cut(s) 333
Cfr13I GGNCC 3 cut(s) 299, 629, 751
CseI GACGC 2 cut(s) 650, 704
Csp6I GTAC 1 cut(s) 469
CspAI ACCGGT 1 cut(s) 333
CviAII CATG 3 cut(s) 118, 766, 820
CviQI GTAC 1 cut(s) 469
DdeI CTNAG 2 cut(s) 383, 747
DpnI GATC 5 cut(s) 11, 199, 212, 564, 672
DpnII GATC 5 cut(s) 9, 197, 210, 562, 670
DraI TTTAAA 1 cut(s) 861
DraIII CACNNNGTG 1 cut(s) 168
EaeI YGGCCR 1 cut(s) 102
Eam1104I CTCTTC 2 cut(s) 422, 533
EarI CTCTTC 2 cut(s) 422, 533
EciI GGCGGA 1 cut(s) 174
Eco147I AGGCCT 1 cut(s) 705
Eco47I GGWCC 3 cut(s) 299, 629, 751
Eco57I CTGAAG 1 cut(s) 683
Eco88I CYCGRG 1 cut(s) 233
EcoRII CCWGG 1 cut(s) 603
EcoT22I ATGCAT 1 cut(s) 825
Esp3I CGTCTC 2 cut(s) 224, 413
FaeI CATG 3 cut(s) 121, 769, 823
FaiI YATR 9 cut(s) 119, 141, 515, 532, 534, 634, 767, 821, 852
FalI AAGNNNNNCTT 2 cut(s) 243, 275
FaqI GGGAC 2 cut(s) 185, 388
FatI CATG 3 cut(s) 117, 765, 819
FauI CCCGC 1 cut(s) 115
Fnu4HI GCNGC 1 cut(s) 441
FokI GGATG 1 cut(s) 319
Fsp4HI GCNGC 1 cut(s) 441
FspBI CTAG 3 cut(s) 450, 545, 614
GluI GCNGC 1 cut(s) 441
HaeIII GGCC 2 cut(s) 104, 705
HapII CCGG 6 cut(s) 105, 302, 322, 334, 472, 719
HgaI GACGC 2 cut(s) 650, 704
Hin1I GRCGYC 1 cut(s) 696
Hin1II CATG 3 cut(s) 121, 769, 823
HincII GTYRAC 3 cut(s) 268, 331, 694
HindII GTYRAC 3 cut(s) 268, 331, 694
HinfI GANTC 1 cut(s) 497
HpaI GTTAAC 1 cut(s) 331
HpaII CCGG 6 cut(s) 105, 302, 322, 334, 472, 719
HphI GGTGA 4 cut(s) 413, 436, 506, 658
Hpy166II GTNNAC 4 cut(s) 230, 268, 331, 694
Hpy188I TCNGA 2 cut(s) 502, 735
Hpy188III TCNNGA 4 cut(s) 223, 638, 674, 713
Hpy8I GTNNAC 4 cut(s) 230, 268, 331, 694
HpyAV CCTTC 1 cut(s) 216
HpyCH4III ACNGT 5 cut(s) 166, 176, 424, 508, 725
HpyCH4IV ACGT 2 cut(s) 264, 681
HpyCH4V TGCA 2 cut(s) 730, 823
HpyF10VI GCNNNNNNNGC 3 cut(s) 440, 446, 623
HpyF3I CTNAG 2 cut(s) 383, 747
HpySE526I ACGT 2 cut(s) 264, 681
Hsp92I GRCGYC 1 cut(s) 696
Hsp92II CATG 3 cut(s) 121, 769, 823
KspAI GTTAAC 1 cut(s) 331
Kzo9I GATC 5 cut(s) 9, 197, 210, 562, 670
LmnI GCTCC 1 cut(s) 623
LweI GCATC 4 cut(s) 64, 297, 511, 739
MaeI CTAG 3 cut(s) 450, 545, 614
MaeII ACGT 2 cut(s) 264, 681
MaeIII GTNAC 1 cut(s) 761
MalI GATC 5 cut(s) 11, 199, 212, 564, 672
MboI GATC 5 cut(s) 9, 197, 210, 562, 670
MboII GAAGA 9 cut(s) 4, 16, 32, 50, 439, 550, 557, 584, 676
MflI RGATCY 1 cut(s) 9
MhlI GDGCHC 1 cut(s) 365
MluCI AATT 2 cut(s) 180, 796
MlyI GAGTC 1 cut(s) 506
MmeI TCCRAC 1 cut(s) 277
MnlI CCTC 8 cut(s) 122, 224, 238, 409, 423, 695, 720, 754
Mph1103I ATGCAT 1 cut(s) 825
MroXI GAANNNNTTC 1 cut(s) 554
MseI TTAA 3 cut(s) 279, 330, 860
MslI CAYNNNNRTG 1 cut(s) 164
MspI CCGG 6 cut(s) 105, 302, 322, 334, 472, 719
MspR9I CCNGG 5 cut(s) 106, 303, 323, 473, 605
Mva1269I GAATGC 1 cut(s) 197
MvaI CCWGG 1 cut(s) 605
MvnI CGCG 1 cut(s) 644
MwoI GCNNNNNNNGC 3 cut(s) 440, 446, 623
NciI CCSGG 4 cut(s) 106, 303, 323, 473
NdeII GATC 5 cut(s) 9, 197, 210, 562, 670
NlaIII CATG 3 cut(s) 121, 769, 823
NlaIV GGNNCC 1 cut(s) 357
NmeAIII GCCGAG 1 cut(s) 799
NmuCI GTSAC 1 cut(s) 761
NsiI ATGCAT 1 cut(s) 825
NspI RCATGY 1 cut(s) 823
NspV TTCGAA 1 cut(s) 568
OliI CACNNNNGTG 1 cut(s) 164
PaeR7I CTCGAG 1 cut(s) 233
PceI AGGCCT 1 cut(s) 705
PcsI WCGNNNNNNNCGW 1 cut(s) 687
PctI GAATGC 1 cut(s) 197
PdmI GAANNNNTTC 1 cut(s) 554
PflMI CCANNNNNTGG 1 cut(s) 168
PinAI ACCGGT 1 cut(s) 333
PkrI GCNGC 1 cut(s) 442
PleI GAGTC 1 cut(s) 505
PpsI GAGTC 1 cut(s) 505
PshBI ATTAAT 1 cut(s) 279
PsiI TTATAA 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 603
PspGI CCWGG 1 cut(s) 603
PspN4I GGNNCC 1 cut(s) 357
PspPI GGNCC 3 cut(s) 299, 629, 751
PspXI VCTCGAGB 1 cut(s) 233
PsuI RGATCY 1 cut(s) 9
RsaI GTAC 1 cut(s) 470
RsaNI GTAC 1 cut(s) 469
RseI CAYNNNNRTG 1 cut(s) 164
SaqAI TTAA 3 cut(s) 279, 330, 860
SatI GCNGC 1 cut(s) 441
Sau3AI GATC 5 cut(s) 9, 197, 210, 562, 670
Sau96I GGNCC 3 cut(s) 299, 629, 751
SchI GAGTC 1 cut(s) 506
ScrFI CCNGG 5 cut(s) 106, 303, 323, 473, 605
SduI GDGCHC 1 cut(s) 365
SfaNI GCATC 4 cut(s) 64, 297, 511, 739
Sfr274I CTCGAG 1 cut(s) 233
SfuI TTCGAA 1 cut(s) 568
SinI GGWCC 3 cut(s) 299, 629, 751
SlaI CTCGAG 1 cut(s) 233
SmiMI CAYNNNNRTG 1 cut(s) 164
SmlI CTYRAG 2 cut(s) 233, 674
SmoI CTYRAG 2 cut(s) 233, 674
SpeI ACTAGT 1 cut(s) 544
Sse9I AATT 2 cut(s) 180, 796
SseBI AGGCCT 1 cut(s) 705
SsiI CCGC 7 cut(s) 53, 122, 132, 185, 440, 476, 644
SspI AATATT 1 cut(s) 865
SspMI CTAG 3 cut(s) 450, 545, 614
StuI AGGCCT 1 cut(s) 705
StyD4I CCNGG 5 cut(s) 104, 301, 321, 471, 603
TaaI ACNGT 5 cut(s) 166, 176, 424, 508, 725
TaiI ACGT 2 cut(s) 267, 684
TaqI TCGA 6 cut(s) 127, 209, 234, 519, 568, 805
TaqII GACCGA 1 cut(s) 749
TasI AATT 2 cut(s) 180, 796
TauI GCSGC 1 cut(s) 443
Tru1I TTAA 3 cut(s) 279, 330, 860
Tru9I TTAA 3 cut(s) 279, 330, 860
TscAI CASTG 2 cut(s) 154, 565
TseFI GTSAC 1 cut(s) 761
Tsp45I GTSAC 1 cut(s) 761
TspDTI ATGAA 3 cut(s) 16, 85, 872
TspGWI ACGGA 2 cut(s) 206, 348
TspRI CASTG 2 cut(s) 154, 565
Van91I CCANNNNNTGG 1 cut(s) 168
VpaK11BI GGWCC 3 cut(s) 299, 629, 751
VspI ATTAAT 1 cut(s) 279
XapI RAATTY 1 cut(s) 796
XceI RCATGY 1 cut(s) 823
XhoI CTCGAG 1 cut(s) 233
XmnI GAANNNNTTC 1 cut(s) 554
XspI CTAG 3 cut(s) 450, 545, 614
Zsp2I ATGCAT 1 cut(s) 825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.