Rh6CG494000

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0011720
Physical Location & Seq
Forward (+)
66003896 .. 66005079
1184 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG494000.1

Sequence Viewer

Length: 894 bp
ATGATGATGCTTTTCCTGTTGTTCTTGTCTCTTGCGTCTCACGCCTCCTCATCAAAAGAGCAACACATCAAAAGCATTTTCATCCTGGCCGGACAGAGCAACATGGCCGGCCGCGGAGGCGTTGTAAACGACACTCTCACCGGAATCACCACCTGGGACGGCGTCGTTCCTCCAGAGTGCCTCCCCAACAAATCCATCTTCCGACTCAGCGCAAACCTCACGTGGGTTGAAGCCCGCGAGCCTCTCCACGCTGATATCGACGTGGAGAAGACGAATGGGATCGGACCCGGGATGCCATTCGCCAGTGCGATCCTTGCCAATCGTGCTAGTAGTATTGGTGCGATTGGTTTAGTCCCGTGCGCAATCGGCGGGACTAACCTAAGCCAATGGGAACGAGGGTCTTTCCTATACAGACAGATGATTGGGAGAGCCAAGGCGGCTTTGTTAAAGGTGAATGATAATGGCGCCTCAATCAGGGGACTACTCTGGTATCAAGGTGAGAGCGATACGACTAGTATAGAAGACGCCCGATCTTACAAAGCCAGATTGGAAACCTTCTTTGGAGACCTCCGGTTGGATCTGCAATTTCCTATGCTTCCAATAATCCAGGTTGCAATTGCATCTGGGTCAGGACCATACATAGAGACCATTAGAGAAGCTCAGATGGGAATTGACCTACTAAACGTGCGAACCGTTGATGCCAAGGGTCTAACATTAGAACCTGATAGGTTGCACCTCACTACCCCGAGTCAGGTTCGACTAGGGGAAATGTTGGCTAACGCATATTTTCAGTTAATTCCCAGCACGATAAGCAATAATGCTCGGAGAAATGGACATCCTTGTTTGATCATCCATTTTGTTATTGGCTTGTTATGGAAAATTTTGACTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

297

Amino Acids

32.38

Weight (kDa)

7.77

Isoelectric Point (pI)

31.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 24 - 264 1.7e-89 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011720)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53010
fragaria_vesca FvH4_2g33800
malus_domestica MD08G1092200.v1.1 MD15G1076700.v1.1
prunus_persica Prupe.1G430100_v2.0.a1
pyrus_communis pycom08g07490 pycom08g07500 pycom15g07150
rosa_chinensis RchiOBHm_Chr6g0308761
rosa_laevigata RLG00000010622
rosa_multiflora Rmu_sc0000871.1_g000025 Rmu_sc0009346.1_g000012
rosa_roxburghii Rroxscaffold_7G00159960
rosa_rugosa Rorug06G0368100
rosa_samantha Rh6AG479200 Rh6BG488800 Rh6CG494000 Rh6DG479600
rosa_wichuraiana Rw6G041770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 361
AccB1I GGYRCC 1 cut(s) 464
AccII CGCG 2 cut(s) 114, 237
AciI CCGC 5 cut(s) 112, 114, 235, 369, 437
AclWI GGATC 3 cut(s) 287, 304, 585
AcoI YGGCCR 3 cut(s) 87, 105, 109
AcsI RAATTY 1 cut(s) 879
AcvI CACGTG 1 cut(s) 222
AcyI GRCGYC 3 cut(s) 162, 465, 525
AfiI CCNNNNNNNGG 4 cut(s) 223, 474, 574, 751
AgsI TTSAA 1 cut(s) 230
AhlI ACTAGT 1 cut(s) 512
AjiI CACGTC 1 cut(s) 262
AjnI CCWGG 3 cut(s) 84, 152, 606
AjuI GAANNNNNNNTTGG 2 cut(s) 543, 575
AluBI AGCT 1 cut(s) 659
AluI AGCT 1 cut(s) 659
Alw26I GTCTC 4 cut(s) 33, 42, 558, 638
AlwI GGATC 3 cut(s) 287, 304, 585
Ama87I CYCGRG 2 cut(s) 287, 745
AoxI GGCC 3 cut(s) 87, 105, 109
ApoI RAATTY 1 cut(s) 879
AspLEI GCGC 3 cut(s) 212, 362, 467
AspS9I GGNCC 2 cut(s) 284, 632
AsuC2I CCSGG 2 cut(s) 288, 289
AsuHPI GGTGA 4 cut(s) 130, 139, 463, 509
AvaI CYCGRG 2 cut(s) 287, 745
AvaII GGWCC 2 cut(s) 284, 632
BaeI ACNNNNGTAYC 2 cut(s) 473, 506
BanI GGYRCC 1 cut(s) 464
BbrPI CACGTG 1 cut(s) 222
BbsI GAAGAC 2 cut(s) 275, 528
BccI CCATC 2 cut(s) 203, 658
BceAI ACGGC 1 cut(s) 175
BciT130I CCWGG 3 cut(s) 86, 154, 608
BclI TGATCA 1 cut(s) 846
BcnI CCSGG 2 cut(s) 288, 289
BcoDI GTCTC 4 cut(s) 33, 42, 558, 638
BcuI ACTAGT 1 cut(s) 512
BfaI CTAG 3 cut(s) 327, 513, 761
BfoI RGCGCY 1 cut(s) 468
BglI GCCNNNNNGGC 2 cut(s) 117, 437
BisI GCNGC 2 cut(s) 112, 438
BlsI GCNGC 2 cut(s) 113, 439
Bme1390I CCNGG 5 cut(s) 86, 154, 288, 289, 608
Bme18I GGWCC 2 cut(s) 284, 632
BmeT110I CYCGRG 2 cut(s) 287, 745
BmgBI CACGTC 1 cut(s) 262
BmgT120I GGNCC 2 cut(s) 284, 632
BmiI GGNNCC 2 cut(s) 286, 466
BmrFI CCNGG 5 cut(s) 86, 154, 288, 289, 608
BmsI GCATC 3 cut(s) 282, 629, 688
BpiI GAAGAC 2 cut(s) 275, 528
BpmI CTGGAG 1 cut(s) 156
Bpu10I CCTNAGC 1 cut(s) 380
BpuMI CCSGG 2 cut(s) 288, 289
BsaAI YACGTR 1 cut(s) 222
BsaHI GRCGYC 3 cut(s) 162, 465, 525
BsaI GGTCTC 2 cut(s) 558, 638
BsaJI CCNNGG 5 cut(s) 112, 153, 287, 432, 702
BsaWI WCCGGW 2 cut(s) 140, 570
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bsc4I CCNNNNNNNGG 4 cut(s) 223, 474, 574, 751
Bse118I RCCGGY 1 cut(s) 107
Bse1I ACTGG 1 cut(s) 303
BseBI CCWGG 3 cut(s) 86, 154, 608
BseDI CCNNGG 5 cut(s) 112, 153, 287, 432, 702
BseGI GGATG 4 cut(s) 81, 297, 835, 849
BseLI CCNNNNNNNGG 4 cut(s) 223, 474, 574, 751
BseMII CTCAG 2 cut(s) 220, 674
BseNI ACTGG 1 cut(s) 303
BseRI GAGGAG 1 cut(s) 37
BseX3I CGGCCG 1 cut(s) 109
BseYI CCCAGC 1 cut(s) 800
Bsh1236I CGCG 2 cut(s) 114, 237
Bsh1285I CGRYCG 1 cut(s) 112
BshFI GGCC 3 cut(s) 89, 107, 111
BshNI GGYRCC 1 cut(s) 464
BsiEI CGRYCG 1 cut(s) 112
BsiHKCI CYCGRG 2 cut(s) 287, 745
BsiSI CCGG 5 cut(s) 90, 108, 141, 288, 571
BslFI GGGAC 4 cut(s) 170, 338, 385, 492
BslI CCNNNNNNNGG 4 cut(s) 223, 474, 574, 751
BsmAI GTCTC 4 cut(s) 33, 42, 558, 638
BsmBI CGTCTC 1 cut(s) 42
BsmFI GGGAC 4 cut(s) 170, 338, 385, 492
BsnI GGCC 3 cut(s) 89, 107, 111
Bso31I GGTCTC 2 cut(s) 558, 638
BsoBI CYCGRG 2 cut(s) 287, 745
Bsp143I GATC 5 cut(s) 279, 309, 530, 577, 846
BspACI CCGC 5 cut(s) 112, 114, 235, 369, 437
BspANI GGCC 3 cut(s) 89, 107, 111
BspCNI CTCAG 2 cut(s) 219, 673
BspFNI CGCG 2 cut(s) 114, 237
BspLI GGNNCC 2 cut(s) 286, 466
BspPI GGATC 3 cut(s) 287, 304, 585
BspT107I GGYRCC 1 cut(s) 464
BspTNI GGTCTC 2 cut(s) 558, 638
BsrFI RCCGGY 1 cut(s) 107
BsrI ACTGG 1 cut(s) 303
BssAI RCCGGY 1 cut(s) 107
BssECI CCNNGG 5 cut(s) 112, 153, 287, 432, 702
BssMI GATC 5 cut(s) 279, 309, 530, 577, 846
BssNI GRCGYC 3 cut(s) 162, 465, 525
BssT1I CCWWGG 2 cut(s) 432, 702
Bst2UI CCWGG 3 cut(s) 86, 154, 608
Bst4CI ACNGT 1 cut(s) 694
BstACI GRCGYC 3 cut(s) 162, 465, 525
BstBAI YACGTR 1 cut(s) 222
BstC8I GCNNGC 3 cut(s) 109, 235, 239
BstDEI CTNAG 3 cut(s) 206, 380, 660
BstDSI CCRYGG 1 cut(s) 112
BstENI CCTNNNNNAGG 1 cut(s) 472
BstF5I GGATG 4 cut(s) 81, 297, 835, 849
BstFNI CGCG 2 cut(s) 114, 237
BstH2I RGCGCY 1 cut(s) 468
BstHHI GCGC 3 cut(s) 212, 362, 467
BstKTI GATC 5 cut(s) 282, 312, 533, 580, 849
BstMAI GTCTC 4 cut(s) 33, 42, 558, 638
BstMBI GATC 5 cut(s) 279, 309, 530, 577, 846
BstMCI CGRYCG 1 cut(s) 112
BstMWI GCNNNNNNNGC 7 cut(s) 41, 117, 314, 323, 366, 437, 810
BstNI CCWGG 3 cut(s) 86, 154, 608
BstSCI CCNGG 5 cut(s) 84, 152, 286, 287, 606
BstUI CGCG 2 cut(s) 114, 237
BstV2I GAAGAC 2 cut(s) 275, 528
BstX2I RGATCY 1 cut(s) 577
BstYI RGATCY 1 cut(s) 577
BstZI CGGCCG 1 cut(s) 109
BsuRI GGCC 3 cut(s) 89, 107, 111
BtgI CCRYGG 1 cut(s) 112
BtrI CACGTC 1 cut(s) 262
BtsCI GGATG 4 cut(s) 81, 297, 835, 849
BtsIMutI CAGTG 1 cut(s) 310
Cac8I GCNNGC 3 cut(s) 109, 235, 239
CfoI GCGC 3 cut(s) 212, 362, 467
Cfr10I RCCGGY 1 cut(s) 107
Cfr13I GGNCC 2 cut(s) 284, 632
Cfr42I CCGCGG 1 cut(s) 115
Cfr9I CCCGGG 1 cut(s) 287
CseI GACGC 3 cut(s) 24, 151, 533
CviAII CATG 1 cut(s) 103
DdeI CTNAG 3 cut(s) 206, 380, 660
DinI GGCGCC 1 cut(s) 466
DpnI GATC 5 cut(s) 281, 311, 532, 579, 848
DpnII GATC 5 cut(s) 279, 309, 530, 577, 846
EaeI YGGCCR 3 cut(s) 87, 105, 109
EagI CGGCCG 1 cut(s) 109
EclXI CGGCCG 1 cut(s) 109
Eco130I CCWWGG 2 cut(s) 432, 702
Eco31I GGTCTC 2 cut(s) 558, 638
Eco32I GATATC 1 cut(s) 256
Eco47I GGWCC 2 cut(s) 284, 632
Eco52I CGGCCG 1 cut(s) 109
Eco72I CACGTG 1 cut(s) 222
Eco88I CYCGRG 2 cut(s) 287, 745
EcoNI CCTNNNNNAGG 1 cut(s) 472
EcoRII CCWGG 3 cut(s) 84, 152, 606
EcoRV GATATC 1 cut(s) 256
EcoT14I CCWWGG 2 cut(s) 432, 702
EgeI GGCGCC 1 cut(s) 466
EheI GGCGCC 1 cut(s) 466
ErhI CCWWGG 2 cut(s) 432, 702
Esp3I CGTCTC 1 cut(s) 42
FaeI CATG 1 cut(s) 106
FaiI YATR 9 cut(s) 104, 409, 518, 593, 637, 641, 784, 874, 892
FaqI GGGAC 4 cut(s) 170, 338, 385, 492
FatI CATG 1 cut(s) 102
FauI CCCGC 2 cut(s) 242, 362
FbaI TGATCA 1 cut(s) 846
Fnu4HI GCNGC 2 cut(s) 112, 438
FokI GGATG 4 cut(s) 68, 304, 822, 836
FseI GGCCGGCC 1 cut(s) 111
Fsp4HI GCNGC 2 cut(s) 112, 438
FspBI CTAG 3 cut(s) 327, 513, 761
FspI TGCGCA 1 cut(s) 361
GlaI GCGC 3 cut(s) 211, 361, 466
GluI GCNGC 2 cut(s) 112, 438
GsaI CCCAGC 1 cut(s) 804
GsuI CTGGAG 1 cut(s) 156
HaeII RGCGCY 1 cut(s) 468
HaeIII GGCC 3 cut(s) 89, 107, 111
HapII CCGG 5 cut(s) 90, 108, 141, 288, 571
HgaI GACGC 3 cut(s) 24, 151, 533
HhaI GCGC 3 cut(s) 212, 362, 467
Hin1I GRCGYC 3 cut(s) 162, 465, 525
Hin1II CATG 1 cut(s) 106
Hin6I GCGC 3 cut(s) 210, 360, 465
HinP1I GCGC 3 cut(s) 210, 360, 465
HinfI GANTC 3 cut(s) 144, 204, 748
HpaII CCGG 5 cut(s) 90, 108, 141, 288, 571
HphI GGTGA 4 cut(s) 130, 139, 463, 509
Hpy166II GTNNAC 1 cut(s) 127
Hpy188I TCNGA 4 cut(s) 203, 284, 663, 825
Hpy188III TCNNGA 2 cut(s) 173, 630
Hpy8I GTNNAC 1 cut(s) 127
Hpy99I CGWCG 2 cut(s) 167, 263
HpyAV CCTTC 1 cut(s) 565
HpyCH4III ACNGT 1 cut(s) 694
HpyCH4IV ACGT 3 cut(s) 221, 261, 684
HpyCH4V TGCA 4 cut(s) 583, 614, 620, 733
HpyF10VI GCNNNNNNNGC 7 cut(s) 41, 117, 314, 323, 366, 437, 810
HpyF3I CTNAG 3 cut(s) 206, 380, 660
HpySE526I ACGT 3 cut(s) 221, 261, 684
Hsp92I GRCGYC 3 cut(s) 162, 465, 525
Hsp92II CATG 1 cut(s) 106
HspAI GCGC 3 cut(s) 210, 360, 465
KasI GGCGCC 1 cut(s) 464
KroI GCCGGC 1 cut(s) 107
KroNI GCCGGC 1 cut(s) 109
Ksp22I TGATCA 1 cut(s) 846
KspI CCGCGG 1 cut(s) 115
Kzo9I GATC 5 cut(s) 279, 309, 530, 577, 846
LweI GCATC 3 cut(s) 282, 629, 688
MaeI CTAG 3 cut(s) 327, 513, 761
MaeII ACGT 3 cut(s) 221, 261, 684
MalI GATC 5 cut(s) 281, 311, 532, 579, 848
MboI GATC 5 cut(s) 279, 309, 530, 577, 846
MboII GAAGA 3 cut(s) 190, 280, 533
MfeI CAATTG 1 cut(s) 615
MflI RGATCY 1 cut(s) 577
MluCI AATT 5 cut(s) 584, 615, 669, 795, 879
Mly113I GGCGCC 1 cut(s) 465
MlyI GAGTC 2 cut(s) 198, 757
MmeI TCCRAC 2 cut(s) 226, 555
MroNI GCCGGC 1 cut(s) 107
MseI TTAA 2 cut(s) 446, 794
MspA1I CMGCKG 1 cut(s) 114
MspI CCGG 5 cut(s) 90, 108, 141, 288, 571
MspR9I CCNGG 5 cut(s) 86, 154, 288, 289, 608
MunI CAATTG 1 cut(s) 615
MvaI CCWGG 3 cut(s) 86, 154, 608
MvnI CGCG 2 cut(s) 114, 237
MwoI GCNNNNNNNGC 7 cut(s) 41, 117, 314, 323, 366, 437, 810
NaeI GCCGGC 1 cut(s) 109
NarI GGCGCC 1 cut(s) 465
NciI CCSGG 2 cut(s) 288, 289
NdeII GATC 5 cut(s) 279, 309, 530, 577, 846
NgoMIV GCCGGC 1 cut(s) 107
NlaIII CATG 1 cut(s) 106
NlaIV GGNNCC 2 cut(s) 286, 466
NsbI TGCGCA 1 cut(s) 361
PcsI WCGNNNNNNNCGW 2 cut(s) 255, 690
PdiI GCCGGC 1 cut(s) 109
PfeI GAWTC 1 cut(s) 144
PflFI GACNNNGTC 1 cut(s) 161
PkrI GCNGC 2 cut(s) 113, 439
PleI GAGTC 2 cut(s) 198, 756
PluTI GGCGCC 1 cut(s) 468
PmaCI CACGTG 1 cut(s) 222
PmlI CACGTG 1 cut(s) 222
PpsI GAGTC 2 cut(s) 198, 756
Ppu21I YACGTR 1 cut(s) 222
Psp6I CCWGG 3 cut(s) 84, 152, 606
PspCI CACGTG 1 cut(s) 222
PspFI CCCAGC 1 cut(s) 800
PspGI CCWGG 3 cut(s) 84, 152, 606
PspN4I GGNNCC 2 cut(s) 286, 466
PspPI GGNCC 2 cut(s) 284, 632
PsuI RGATCY 1 cut(s) 577
PsyI GACNNNGTC 1 cut(s) 161
RigI GGCCGGCC 1 cut(s) 111
SacII CCGCGG 1 cut(s) 115
SaqAI TTAA 2 cut(s) 446, 794
SatI GCNGC 2 cut(s) 112, 438
Sau3AI GATC 5 cut(s) 279, 309, 530, 577, 846
Sau96I GGNCC 2 cut(s) 284, 632
SchI GAGTC 2 cut(s) 198, 757
ScrFI CCNGG 5 cut(s) 86, 154, 288, 289, 608
SfaNI GCATC 3 cut(s) 282, 629, 688
SfoI GGCGCC 1 cut(s) 466
Sfr303I CCGCGG 1 cut(s) 115
SgrBI CCGCGG 1 cut(s) 115
SinI GGWCC 2 cut(s) 284, 632
SmaI CCCGGG 1 cut(s) 289
SpeI ACTAGT 1 cut(s) 512
Sse9I AATT 5 cut(s) 584, 615, 669, 795, 879
SsiI CCGC 5 cut(s) 112, 114, 235, 369, 437
SspDI GGCGCC 1 cut(s) 464
SspMI CTAG 3 cut(s) 327, 513, 761
StyD4I CCNGG 5 cut(s) 84, 152, 286, 287, 606
StyI CCWWGG 2 cut(s) 432, 702
TaaI ACNGT 1 cut(s) 694
TaiI ACGT 3 cut(s) 224, 264, 687
TaqI TCGA 2 cut(s) 258, 757
TasI AATT 5 cut(s) 584, 615, 669, 795, 879
TauI GCSGC 2 cut(s) 114, 440
TfiI GAWTC 1 cut(s) 144
Tru1I TTAA 2 cut(s) 446, 794
Tru9I TTAA 2 cut(s) 446, 794
TscAI CASTG 1 cut(s) 310
TspDTI ATGAA 1 cut(s) 70
TspMI CCCGGG 1 cut(s) 287
TspRI CASTG 1 cut(s) 310
Tth111I GACNNNGTC 1 cut(s) 161
VpaK11BI GGWCC 2 cut(s) 284, 632
XagI CCTNNNNNAGG 1 cut(s) 472
XapI RAATTY 1 cut(s) 879
XcmI CCANNNNNNNNNTGG 1 cut(s) 860
XmaI CCCGGG 1 cut(s) 287
XspI CTAG 3 cut(s) 327, 513, 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.